wp2-p6-inra leafyveg-12december2007 activity of the p6 in the frame work of the project:...

LeafyVeg-12December2007WP2-P6-INRA

Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions

WP 2 – P6 (INRA-France)

Objectives for year 1: regeneration of 75 L. sativa


Work made at INRA in year 1

Regeneration of lettuce accessions

Control of the harvested seedsQuality : germination

Seed production under insect proof plastic tunnelStorage of seeds in 2 conditions (6° and -18°C)

Purity : homogeneity of progeny by observation of morphological type

Insect-proof tunnel

Control of winter cultivars


Choice of 300 accessions for regeneration

Old public cultivars Genotypes from public lab (USDA, US universities,

…) Cultivars authorized by breeding companies Cultivars received more than 20 years ago without

restriction

Accessions free for seed distribution

300 accessions multiplied in 2006-2010


Regeneration of lettuce in 2007

Culture in 2007: 38 accessions (with 2 plants) Sowing of old seeds 23 February Plantation 10 April Notation of the minimum descriptors for

flowering Harvesting plant by plant from July to

August(10-40 g/plant)

Storage of seed samples at -18°C 7 December (about 2 x 5-6 g/accession)

Insect-proof tunnel used for seed

production


Culture in field in 2007 : accessions harvested in 2006

Control in 2007 of harvested seeds

Sowing 16 March Plantation 12 April Notation of the minimum descriptors for vegetative

characters and pictures May

Culture in tunnel in 2007: winter accessions harvested in 2006 or 2007

Sowing 14 September Plantation 4 October Notation of the minimum descriptors for vegetative

characters and pictures November-December


Winter tunnel in November 2007

Total for the LeafyVeg project

Each accession: 2 plots of 32

plants

Other accessions are recent cultivars

12 batavia 131 butterhead 198 leaf lettuce 1 1 cos


Control of winter Butterhead lettuce

cv GX535

cv Bremex

Old cultivars


Control of winter Butterhead lettuce

cv Octavo (RZ)

cv Pegase (Vilmorin)

Modern cultivars from breeding companies


Control of winter lettuce

Pictures for accession characterization

Batavia Verdie

Butterhead Magdalena

Butterhead PégaseButterhead Méliès

Butterhead Raphaela

Butterhead Tekla


Choice of the 300 accessions for ILDB (sent to Rob)

Regeneration of 42 accessions and storage of the seeds

Control of harvested seedsin open field = 27 open field accessions harvested in 2006 under plastic tunnel = 21 winter accessions harvested in 2006-2007

Notation of minimum descriptors and photos

Translation of the press release for publication in French agricultural journals (in collaboration with Valérie)

Conclusion of the year 1 for P6

Activities made in 2007


Copy in Excel format of the minimum descriptors to include in ILDB (+ some other descriptors and photos?)

Possibility to choose the other free accessions of the lettuce (L. sativa) INRA collection to include in ILDB

Conclusion of the year 1 for P6

Work still to be doneActivities planned in LeafyVeg project

Complete the passport data and send to Rob

Other activities

wp2-p6-inra leafyveg-12december2007 activity of the p6 in the frame work of the project:...

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