wp 2 tasks: 2-1 to 2-3 task leader: dimitra milioni

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C R O P S T O I N D U S T R Y C R O P S T O I N D U S T R Y WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni Crops2Industry Crops2Industry “Non-food Crops-to-Industry schemes in EU27”

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WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni. Crops2Industry “Non-food Crops-to-Industry schemes in EU27”. Work Package 2: Plant breeding. - PowerPoint PPT Presentation

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Page 1: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

WP 2Tasks: 2-1 to 2-3

Task leader: Dimitra Milioni

Crops2IndustryCrops2Industry“Non-food Crops-to-Industry

schemes in EU27”

Page 2: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

•Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing•Task 2.2: To identify European Genetic Resource Centres •Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement

Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints

Work Package 2: Plant breeding

Page 3: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Varshney et al., 2010

Spanning the gap between genomics and breeding.

Page 4: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

•Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing•Task 2.2: To identify European Genetic Resource Centres •Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement

Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints

Work Package 2: Plant breeding

Page 5: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

European Genetic Resource Centres

European Cooperative Crop Genetic Resources Program (ECPGR)

EURISCO, a web-based catalogueCentre for Genetic Resources, The Netherlands (CGN) Centre for Plant Genetic Resources Poland, Blonie, Poland Istituto di Genetica Vegetale (Germplasm Research Institute),

Italy The Nordic Gene Bank, Alnarp, Sweden The Research Institute of Crop Production, Czech RepublicUK Plant Genetic Resources Group, UK Germplasm Resources Information Network (GRIN), USA

Germplasm as a starting point

Page 6: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Page 7: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Molecular Genomics Resource CentresNot one gene but all the genes

European Molecular Biology Laboratory – European Bioinformatics Institute (EMBL-EBI)

National Centre for Biotechnology Information (NCBI)

DNA Databank in Japan (DDBJ)

The Institute for Genome Research (TIGR)

PlantGDB

Brassica Genome Gateway

GRAMENE

Page 8: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Page 9: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Page 10: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

AH Paterson et al. Nature 457, 551-556 (2009) doi:10.1038/nature07723

Orthologous gene families between sorghum, Arabidopsis, rice and poplar.

Whole genome shotgun sequencing project

Page 11: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

AH Paterson et al. Nature 457, 551-556 (2009) doi:10.1038/nature07723

Alignment of sorghum, rice and maize.

Page 12: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Molecular markers to study genetic diversity in sogrhum germplasms

Simple Sequence Repeats (SSR)

Inter Simple Sequence Repeats (ISSR)

Random Amplified Polymorphic DNA (RAPD)

Amplified Fragment Length Polymprphism (AFLP)

Diversity Arrays Technology (DArT)

Single Feature Polymorphism (SFP)

Quantitative Trait Locus (QTL)

Page 13: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench

Shiringani et al., TAQ (2010); 121(2):32336.

Page 14: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

An Induced Sorghum Mutant Population

Section of the leaf blade from BTx623, P5A3, and P7H6Gallery of selected mutant phenotypes

Page 15: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Production of transgenic sorghum plantsPlant Cell, Tissue and Organ CulturePlant Cell, Tissue and Organ Culture

doidoiI: 10.1007/s11240-007-9228-1I: 10.1007/s11240-007-9228-1 TRANSGENIC SWEET SORGHUM WITH ALTEREDLIGNIN COMPOSITION AND PROCESS OF PREPARATION THEREOF (United StatesPatent Application 20100058496)

Genetic transformation of Sorghum

Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus).

Page 16: WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni

C R O P S T O I N D U S T R YC R O P S T O I N D U S T R Y

Thank you for your attention

Crops2IndustryCrops2Industry“Non-food Crops-to-Industry

schemes in EU27”