workshop in diagnostic lung cancer: pd-l1 testing, nordiqc eqa · 2019-03-19 · lung cancer: pd-l1...
TRANSCRIPT
Lung cancer:PD-L1 testing, NordiQC EQA
Workshop in Diagnostic Immunohistochemistry Oud St. Jan/ Old St. John – Brugge (Bruges), Belgium June 13th –15nd 2018
Rasmus Røge, MD, NordiQC schemeorganizer
NordiQC external QA PD-L1
• Two pilot runs with 10 labs • All NordiQC participant invited for new ”Companion module”• First run in Spring 2017• Three ”official” runs (C1-C3) and 1 supplementary run (C1x)• Participants from more than 25 countries
Development in pass rates
C1 C2 C3
Participants 68 145 146
Pass rate 50 % 84 % 91 %
Optimal 37 % 59 % 74 %
Development in pass rates
C1 C2 C3
Participants 68 145 146
Pass rate 50 % 84 % 91 %
Optimal 37 % 59 % 74 %
Optimal Insufficient
Core 17:TPS: > 50%
Core 19:TPS: > 50%
Core 17:Significantloss of cells
Core 19:Significantloss of cells
pharmDx IHC PD-L1, SK006, mAb 22C3 LDT, mAb 22C3, Benchmark
PD-L1 staining with TSA (tonsil)
Optimal staining without amplification Optimal staining with amplification
PD-L1 staining with TSA
Courtesy of O. Nielsen
LDT protocol:mAb 22C3 concVentana BencmarkOptiviewTyramide amp.
Optimal Insufficient
Core 9:TPS: 1-49%
Core 10:TPS: > 50%
Core 9:Negative
Core 10:Significantloss of cells
pharmDx IHC PD-L1, SK006, mAb 22C3,Recommended protocol
pharmDx IHC PD-L1, SK006, mAb 22C3, Short HIER, Envion
Tonsil
• No staining reaction in the vast majority of lymphocytes including mantle zone and germinal centre B-cells
• A weak to moderate, typically punctuated membranous staining reaction of the majority of germinal centre macrophages
• A moderate to strong staining reaction of the majority of epithelial crypt cells.
• No staining reaction in superficial epithelial cells
Low-level expressor
High-level expressor
PD-L1 Horizon cell lines• 1) Strong staining in all cells
• 2) Weak to moderate staining in the majority of cells
• 3) Weak staining in majority of cells
• 4) Negative staining in all cells
Core 2(majorityweak to moderatestainin)
Optimal Poor
Core 2(significantloss of cells)
Core 3(majority, weakstaining)
Core 3(significantloss of cells)
TPS:100% TPS:100%
TPS:100% TPS:100%
TPS:40% TPS:40%
TPS:40% TPS:30%
TPS:1% TPS:1%
TPS:1% TPS:0%
Optimized protocols 22C3A) Dako Autostainer (pharmDx)B) Dako OmnisC) Ventana BenchmarkD) Leica Bond
Conclusions
• ”Best practice” PD-L1 22C3 protocols identified• Variations in TPS score between the different stainer platforms• Overall, Leica Bond platform produced slides with marginally lower
TPS• However, concardance in TPS categories was excellent