Working with real-time sensing for oil & gas operations: emphasis on biosensing

MTS subsea leak detection conference, Houston November 18-19, 2014

thierry.baussant@iris.no

(in Paul et al., Oceanography, 2007)

Image courtesy of the Center for Environmental

Visualization, Univ. Washington

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• 220 employees including 185 researchers, • teaming up with scientific personnel at UiS to a

combined team of 500 scientists

• Head office in Stavanger, department in Bergen, Oslo, subsidiaries in Moscow and Rio de Janeiro

• Unique test and laboratory facilities

• HSE philosophy of zero accidents and ISO standards

• 94 % project financed

• Focus areas

– Automated drilling

– Multiphase reservoir flow – IOR/EOR

– Environmental management - effects - risk and monitoring

– Safety and risk

› Growing areas

› Innovation

– Biotechnology

– Carbon Capture and Storage

40 years of Research and Development

Energy

Environment

Social Science

UK iMON 10-12 September 2013 2

› Being there - acquisition and processing of data without requiring a human presence

› Legislative compliance with environmental policy (e.g. ”No harmful discharges”, low environmental footprint)

› Operational needs : time to detection time to response

› Overcome difficulties related to challenging operationsin Arctic or Deep-sea

› Tools for data visualization and

decision support

Why ”sensing”

UK iMON 10-12 September 2013 3

Large realm of cutting edge technologies for O&G

› RAMAN

› MASS SPECTROMETRY

› CYTOMETRY

› BIOSENSOR

› SENSOR PLATFORM

• AUV/AUV glider

• ROV

• BIOTAGUARD

• LANDERS

MBARI’s «gulper»

BiotaguardSediment Profile Imagery (SPI) CEFAS

MBARI’s Raman DORISS

MBARI’s Benthic rover

HUGIN 1000 Kongsberg

CYTOBUOY

ON 10-12 September 2013 4

General statement for marine sensors

5

Physical sensors >> Chemical sensors >> Biological sensors

TRL+ -

Acoustic

O2

cameras

Sal, Temp & Pres

•Mass Spectrometry

•Raman Spectroscopy

•Surface Plasmon Resonance

sniffers

microbes

algaeDNA

musselUK iMON 10-12 September 2013

Constructed Bacterial Biosensors in collaboration with Lausanne Univ.

8. desember 2014 6

operon

promoter

plasmid

luciferase `reporter´gene

E. coli cell

(biosensor)

isolation of bacteria

able to degrade

’target compound’

Gene R Gene B Gene CGene A

DNA

target

Constructed biosensors:DNA chip for micropolutants detectionIn collaboration with Magellium & Institut de Pharmacologie et Biologie Structurale (CNRS-IPBS) (France)

Project development

Natural living organisms as sensorsBehaviour, response, presence and shift

• Effective integrators and indicators of environmental conditions

• Directly relevant for environmental risk and mitigation actions

• Behaviour, responses, modifications can provide quick and directevidence of water quality change

• By nature long-standing capacity

• Taking the pulse of the ocean

The BiotaGuardplatform

Early warning leakdetection Superior sensitivity

compared to field proven conventional sensors

Quick response to reduce the consequences of an environmental incident

Pinpointing the source by eliminating noise

Long term environmental effect monitoring Unique ability to respond to

a mixture or “cocktail” of contaminants

Continuous monitoring in real-time enables detection of chronic effects

Factual based response to strict regulation

Creating baselines for the environment

Versatile deployment Bottom mounted

Cable-based

Fixed mooring

Buoys: surface, water column

Biology online Direct access to response of

marine organism

Realtime information for decision-making

Cyprines

valve gape

response to

oil exposure

UK iMON 10-12 September 2013 11

Cyprines

valve gape

response to

CO2 leakage

exposure

Baseline

CO2

Deep-sea sponge: pumping activity sensing

Deep-sea corals –Polyp activity sensing

8. desember 2014

On Off On OffPre-E

20 ppm

drill cuttings

exposure

#1

#2

#1 #2

Cora

l poly

pactivity

communication

Expert analytical unit

Operational centre

Coral silhouettecalculated

Time-series of polypactivity with time

ALARM LEVEL WARNING SYSTEM

Lightening

Lightening

HD low lightsensitive camera

Amovible coralRIG

BenthicRIG

structure

Coral RIGw/biosensor

SEAFLOOR

VICINITY ofCORAL REEF

PAMS conceptual units

Picture processingunit – time lapse

ContextualSensor e.g. turbidity, CTD

Batt-eries

Coral 1

Coral x

PAMS

Coral 1

Coral x

SE 1450988-9, Biosensor device, system and method for monitoring a deep-water sea-floor Swedish priority patent application 26.08.2014

Environmental Sample Processor – ESP developed at MBARIReal-time bioindicator species detection

8. desember 2014 15

Image: Holly Bowers © 2014 MBARI

«Lab in a can»

Harmful Algae Invertebrate Larvae

The ESP can detect a wide range of targets

Balanus glandula

(Acorn barnacle)

Pseudo-nitzschia sp.

(toxic & nontoxic)

Heterosigma akashiwo

(& other raphidophytes)

Microbes

Carcinus maenus sp.

(Green crab)

Osedax

Karenia sp.

Mytilus sp.

(Shore mussels)

Polychaete

Alexandrium tamarense/

catenella

Haywood et al. 2007 Journal of Phycology

Jones et al. 2008 Molecular Ecology Notes

Mikulski et al. 2008 Harmful Algae

Preston et al. 2009 Environmental Microbiology

Toxins

Multi-species detection on a single probe arrayfrom a seawater sample

8. desember 2014 17

Molecular probing analysis in situ

(qPCR & SHA)

SHA vizualization

The primary objective of this project is to adapt an Environmental

Sampling Processor (ESP) developed at MBARI to the real-time

detection of targeted oil-degrading bacteria to track hydrocarbon leak

and monitor operational sites.

MOAB

Leak detection

Operational aspects in Northern areas

INTSOK Murmansk 17 September

18

2012-2015

Succession of Hydrocarbon-Degrading Bacteria following hydrocarbons release

“The deep ocean microbial community is highly sensitive to the presence

of released hydrocarbons and is an effective biosensor for

contamination in this environment.” (Dubinsky et al., 2013; Environmental

Science & Technology)

Deepwater Horizon Oil Spill

Focus on limited # of targetswith broad taxonomic affiliation

First application of qPCR belowthe ocean surface (to 1600m+)

Ecogenomic sensor conceptvalidation

Existing 2G ESP

ESP Development Trajectory

3G ESP under development now

Enable mobile operations

Expand repertoire ofanalytical modules

Focus on larger # of targets

3G ESP/Long-Range AUV

28” Extension

119” Vehicle

ARCTool - Develop an AUV front modulefor hydrocarbons monitoring under ice

Sensor payload with underwater MS and

autosampler for seawater collection.

Collaboration with Spyglass (San Francisco, US)

Test in relevant cold

Arctic water conditions

at the IRIS facility

Project pending

(Bluefin BF-12)

O&G Lab

Automation

Spyglass Mass Spec

Spyglass Sampler

AUV with automated

positioning

High Content

Chem/Bio Info

1.Sampling

2. Sensing

3a. Sampler And MS

3b. Prep Into MS

Integrated SPR Chip

GUI with 12-channel readout, each trace is an average of 3 channels9”x7”x4’ Portable SPR System

Work initiated

2G-ESP

targeting oil-

degrading

microbes in cold

Arctic regions

Future LR-AUV 3G-ESP

real time oil detection and mapping

with MS, fluorometer and autosampler

Future modular LR-AUV based

platform with contextual and

genosensor payload

• Monitoring, understanding ocean & surveillance

• Accidental event - oil leakage/subsea spill

• Oil and gas seep search/characterization (production)

• Subsea Infrastructure management (operational)

• Microbially influenced corrosion (MIC)

• Characterization of well properties

Identifying oil signatures

in deep-sea microbial

communities

O&G subsea

challenges

Conclusions

› Numerous conceptual and off-the-shelf technologyopportunities exist for biosensing including for leak detection

› Access to real-time data on target species/substance abundance and presence is possible

- space + time

› Understanding the impacts/consequences of ephemeral events can additionally be done

› Integration of physical, chemical and biological sensing in situ is the thing ! link to modelling and risk assessment

› Thank you

• NDP O&G companies for their support in the «State of the Art Study Deep Water Remote Sensing and Monitoring»

• ConocoPhillips & Lundin Norge and Research Council of NorwayMOAB «Leakage detection» ESP

• Total d-MOAB pre-project

› Thank you to MTS Houston organizers

http://spyglasswater.com/http://www.seattlesensors.com/index.html

thierry.baussant@iris.no