Word of the Day:

Enzyme:An enzyme is a substance that starts a chemical reaction in cells

Enzyme Chemical acted upon

Product

lipase Fats and oils Glycerol and fatty acids

Protease Proteins Amino acids

Catalase Hydrogen peroxide

Water and oxygen

What does your spit do to food?

Amylase Lab

Background

• Amylase is an enzyme found in your saliva

• Amylase breaks down starches and turns them into sugars

Remember your Latin and Greek

• Starch -------------> glucose + glucose + glucose +…

Amylase enzyme

Found in saliva

Substrate

Type of starch

Product

Type of sugar which your body uses for energy

Let’s see how it really works…

• Step 1: Preparing the Lab– Get 10 test tubes– Label 1-10– Add 5 drops of

iodine to each test tube 1

2 dropper fulls of iodine

1

Step 2: Prepare the saliva solution

• Collect 4 ml of saliva -– Spit through the straw into a

clean graduated cylinder– Add 17 ml of

water to graduated

cylinder and mix.

Use straw to collect saliva

Step 3: Prepare the Starch Solution

• Using a 2nd graduated cylinder, get 7ml of starch solution and pour into a small beaker

Measure 7ml of starch solution into beaker

Step 4:

• Mix the saliva solution into the starch solution

• Record the exact time of mixing

Saliva solution

Starch solution

Step 5: Add Saliva solution to Test Tube #1

• Add 10 drops of saliva/starch to test tube # 1

• Record color: – Black/Purple: starch present– Yellow/Brown: no starch present

Step 6: Repeat Step 5, Every Three Minutes

• Every three minutes, add 10 drops of saliva-starch solution to a new test tube– Record color

– Repeat until the starch is no longer present

Data Table

Time Starch Present (Yes/No)

0

3

6

9

12

15

18

Claim, Evidence and Explanation

• Write a CEE

• Due Next Class