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Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals http:// pestsurvey.wi.gov / Photo credit: Anette Phibbs, DATCP Bleeding Heart, Dicentra sp. Tobacco rattle virus (TRV) infections were determined by nucleic assay, reverse transcription polymerase chain reaction (RT-PCR) at DATCP’s Plant Industry Laboratory. References: D.J. Robinson, Journal or Virological Methods. 1992, 40, 57-66.” Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction.”

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Page 1: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Wisconsin Department of Agriculture, Trade and Consumer Protection

Plant Industry Laboratory ReportPlant Industry Laboratory Report2010 Tobacco Rattle Virus Infected Ornamentals

http://pestsurvey.wi.gov/

Photo credit: Anette Phibbs, DATCP

Bleeding Heart, Dicentra sp.

Tobacco rattle virus (TRV) infections were determined by nucleic assay, reverse transcription polymerase chain reaction (RT-PCR) at DATCP’s Plant Industry Laboratory.

References: D.J. Robinson, Journal or Virological Methods. 1992, 40, 57-66.” Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction.”

Page 2: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Astilbe infected with TRV and CMV

Astilbe arendsii “Fanal”

Page 3: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Larkspur infected with TRV

Delphinium elatum “Lilac Pink” and

“Magic Fountain” dark blue/white beeDelphinium elatum “Magic Fountain” pure white

Page 4: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Larkspur infected with TRV

Page 5: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Oriental Lily infected with TRV

Lilium oriental “Angelique”

Page 6: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Hostas infected with TRV

Hosta Funkia “Magic Fire”

Hosta Funkia “Krossa Regal”

Page 7: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Grapeleaf Anemone infected with TRV

Anemone tomentosa “Robustissima”

Page 8: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Barrenwort infected with TRV

1) Epimedium pubigerum “Orange Queen”

2) Epimedium pinnata elegans

3) Epimedium X rubrum

1

2

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Page 9: Wisconsin Department of Agriculture, Trade and Consumer Protection Plant Industry Laboratory Report 2010 Tobacco Rattle Virus Infected Ornamentals

Virginia Bluebells with TRV

Mertensia virginica