why we need and use lcms
DESCRIPTION
Inam's Dummy - Use of LCMSTRANSCRIPT
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LC/MS
Why is it the fastest growinganalytical technique ?
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Discussion topics
◗ Evolution of LC/MS
◗ Advantages of API
◗ Why should I use LC/MS ?
◗ LC/MS markets
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1970s to Present
Moving belt interface (EI and CI, library searchable)
Dynamic FAB (low flow rates, very fiddly)
TSP ionisation (first widely used LC/MS interface)
Atmospheric Pressure Ionisation (ESI and APCI)
Evolution of LC/MS interfaces
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Soft ionisation (gives the molecular weight)
Sensitive (low pg amounts routinely)
Robust, simple, run routinely 24 hr/day
Wide range of flow rates (nanospray to analytical)
Wide range of applications (drugs, proteins)
Wide range of industries
Advantages of API
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API Publications
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1991 1992 1993 1994 1995 1996 1997
Halket JM and Down S, LC/MS Update, HD Science, Nottingham
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LC/MS for shorter analysis times
Example :
MS vs photo diode array detector for the
analysis of steroids
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Minutes
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
-100
0
100
200
300
400
500
600
hydrocortisone
1.9
2.7
progesterone4.6
deoxycorticosterone
methyltestosterone
1.0 mL/Min60/40 MeOH/Water/1% HOAC4.6 mm X 3.3 C18 column
0.8
Steroid standards, 25ng injected
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hydrocortisonedeoxycorticosterone
methyltestosterone progesterone
PDA spectra of standard steroids
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Rela
tive
Abu
ndan
ce331
deoxycorticosterone
Rela
tive
Abu
ndan
ce
363hydrocortisone
Rela
tive
Abu
ndan
ce
303methyltestosterone
Rela
tive
Abu
ndan
ce
315progesterone
MS Spectra of steroids
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What happens if we speed up the chromatography ?
(from 60/40 to 90/10 MeOH/Water)
LC/MS for shorter analysis times
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Detection by UV
Steroids coelute
No distinguishing UV spectra
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hydrocortisone
300 320 340 360 380 400m/z
Rel
ativ
e A
bund
ance
303
315
363
331
progesterone
methyltestosterone
deoxycorticosterone
Detection by MS
363
331
315
303
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LC/MS shorter run times
◗ Changing from 6 to 2 min / sample means :
– 10 samp / hr 30 samp / hr– 80 samp / day 240 samp / day– 20,000 samp / yr 60,000 samp /yr
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Did This Horse Win The RaceDid This Horse Win The Race
LegallyLegally ??
A wide variety of drugs are dosed to horses to enhance performance during racing.
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Thermo Separation ProductsFrequently used analgesics
CH3
OH3C
OH
O
Naproxen
NN
O
O
H3CPhenylbutazone
HN
HO O
Cl
C l
CH3
Meclofenamic Acid
NHN
CH3
CF3
COOH
Flunixin
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It has the correct retention time by UV
Is this peak Flunixin ?
Minutes
mAU Flunixin ?
3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 5.2 5.4 5.6 5.8 6.0 6.2 6.4
80
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140
160
180
254 nm254 nm
NHN
CH3
CF3
COOH
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LC/MS confirms it to be Flunixin
Flunixin Full Scan MS
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0Time (min)
100
0102030405060708090
Rel
ativ
e Ab
unda
nce
100 120 140 160 180 200 220 240 260 280 300 320 340
m/z
0
20
40
60
80
100
297
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Why should I use LC/MS ?
◗ Higher sample throughput– $
◗ Shorter method development– $
◗ Better sensitivity– environmental, legislation
◗ Unequivocal ID– safety
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LC/MS markets
Who uses LC/MS ?
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LC/MS in the pharmaceutical market
◗ Drug discovery– molecular weight, structural– open access– combinatorial chemistry
◗ Metabolism– structural identification and quantification of
metabolites
◗ Toxicology– quantitation
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LC/MS in the pharmaceutical market
◗ Pharmacokinetics– quantitation, sensitivity,precision and accuracy– pre-clinical and clinical studies
◗ Formulation– structural, degradation products
◗ QC & Production– quantitation – ID of impurities & unexpected peaks
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LC/MS in pharmaceutical related markets
◗ Contract research organisations– quantitation, pre-clinical & clinical trials– structural studies
◗ Generic drug companies– QC and production
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LC/MS in the biotechnology market
◗ Protein characterisation– molecular weight, (3D structure)
◗ Proteomics– rapid peptide sequencing – post translational modifications
◗ QC – confirm sequence & impurities
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LC/MS in the biotechnology market
◗ Nucleotides– molecular weight, sequence
◗ Carbohydrates– molecular weight, sequence
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LC/MS in the agrochemical market
◗ Compound discovery
◗ Metabolism
◗ Toxicology
◗ Pharmacokinetics
◗ QC and production
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LC/MS in industrial markets
◗ Organometallics– structure
◗ Detergents– QC, competitors products
◗ Polymers– molecular weight, structure
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LC/MS in the environmental market
◗ Water– ID and quantitation of pollutants
◗ Food – chemical contaminants– natural toxins
◗ Animal feeds– contaminants, illegal substances
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LC/MS in the forensic market
◗ Scene of crime– illegal substances, toxic agents
◗ Horse race doping– illegal substances
◗ Explosives
◗ Drugs of abuse– urine, hair, banknotes
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LC/MS in clinical markets
◗ Replace immuno assays
◗ Drugs of abuse
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LC/MS in academia
◗ Related to all of the above
◗ Fundamental research
◗ Teaching
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Predicted market for LC/MS systems
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1985 1990 1995 2000 2005 2010 2015 2020
Inst
rum
ent s
ales
($ m
illio
n)
LC/MS
1997- 2007
Willoughby R, Sheenan E and Mitrovich S, “A Global View of LC/MS”, Global View Publishing (1998)
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Conclusion
◗ LC/MS is an established technique
◗ Market is growing rapidly
◗ LC/MS moving out of the specialised labs into every department
◗ Wide variety of LC/MS analyser types
◗ Non-specialist users