why d(^lwfe[perts call sequencher™ the best software...
TRANSCRIPT
Why d(^lWfe[perts call Sequencher™ the best software available for sequencing DNA?
Seqpttf&her, by Gene Codes Corporation, is the state-of-the-art in coS^k^T software for sequencing. Sequencher is more than just another sequence analysis program. It represents revolutionary
advances in user-interface design, speed and accuracy, and integration of analysis functions. With Sequencher, your "Sequencher has provided us with an easy to
use and effective approach to editing our sequences, assembhng the data into easily edited contigs, and archiving the trace data...The other thing I appreciate is the level of technical support... [Gene Codes] set new standards for the term 'customer satisfaction.'"
Audrey Goddard Head, DNA Sequencing Core Genentech, Inc.
"An exceptional sequence assembly program...Their response time with fixes or enhancements is nothing short of miraculous..."
Harry Mangalam Department of Biocomputing The Salk Institute
"/ had been spending 3 to 4 hours a day on analysis. Since the day I received Sequencher, I have spent 1/2 hour on average. Sequencher has freed me from most of the tedious parts of analysis, and allows me to draw conclusions from my data much more quickly."
Steve Ellis Millennium Pharmaceuticals, Inc.
"After demos of all of them...Sequencher is the best. Nothing else came close."
Paul Morrison Dir., Molecular Biology Core Facility Dana-Farher Cancer Institute
"Gene Codes has raised my standards for what to expect from a software company."
Naomi Thomson Molecular Biology Department Bristol-Myers Squibb
"Oh boy!" Ripley Parrot Genome Proponent Gene Codes Corporation
Reader Service No. 460
lab will increase productivity, reduce analysis time and tnaxifnize the integrity and quality of your results.
Contig assembly and editing functions are exceptional. And Sequencher's in depth sequencing support includes vector screening, ORF reports^ protein translations, re
striction maps and a fantastic interface to popular fluorescent sequencers.
Gene Codes' unmatched level of support includes program
'^ updates with the features you need. For example • Trim the
I low-quality ends from your ^ fragments, build your contigs
and then recover the data you masked off. • Choose either 'incremental' or 'exhaustive' comparisons, adding fragments to existing projects without losing edits, or re-comparing all old and new data for optimal assembly order. • Export results in the file formats that work best ior you; If you need a new format, ask us.
iiPfew in '95, Sequencher 3.0 has been accelerated for power Macintosh, and has more new features...
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...try it for yourself: Call for a demo disk includingyree restriction mapping software.
Gene Codes Corporation 2901 Hubbard Road Ann Arbor, MI 48105 phone: 313-769-7249 or 800-497-4939 fax:313-769-7074
X C A A G T
G E N E C O D E S
GEISES
DEVELOFMEIST VOLUME 9 NUMBER 19 PAGES 2325-2444 October 1, 1995
EDITORIAL BOARD
J. Adams (Melbourne, Australia) J. Beckwith (Boston, USA) A. Berns (Amsterdam, The Netherlands) E. Blackburn (San Francisco, USA) T. Cech (Boulder, USA) P. Chambon (Strasbourg, France) N.-H. Chua (New York, USA) E. Coen (Norwich, UK) S. Courtneidge (Heidelberg, FRG) R. Evans (La Jolla, USA) G. Fink (Cambridge, USA) P. Goodfellow (Cambridge, UK) S. Gottesman (Bethesda, USA) T. Graf (Heidelberg, FRG) C. Gross (San Francisco, USA) R. Grosschedl (San Francisco, USA) F. Grosveld (Rotterdam, The Netherlands) M. Groudine (Seattle, USA) L. Guarente (Cambridge, USA) R. Harland (Berkeley, USA) E. Harlow (Charlestown, USA) W. Herr (Cold Sprmg Harbor, USA) I. Hodgkin (Cambridge, UK) R. Horvitz (Cambridge, USA) P. Ingham (London, UK) T. Jessell (New York, USA) N. Jones (London, UK)
J. Kadonaga (La Jolla, USA) R. Lehmann (Cambridge, USA) M. Levine (San Diego, USA) D. Livingston (Boston, USA) R. Losick (Cambridge, USA) J. Manley (New York, USA) W. McGinnis (New Haven, USA) S. McKnight (South San Francisco, USA) R. McKay (Bethesda, USA) A. McMahon (Cambridge, USA) P. Nurse (London, UK) R. Palmiter (Seattle, USA) L. Parada (Dallas, USA) C. Prives (New York, USA) G. Rubin (Berkeley, USA) U. Schibler (Geneva, Switzerland) P. Sharp (Cambridge, USA) C. Sherr (Memphis, USA) D. Sherratt (Oxford, UK) D. Solter (Freiburg, FRG) P. Soriano (Seattle, USA) J. Steitz (New Haven, USA) M. Takeichi (Kyoto, Japan) T. Taniguchi (Tokyo, Japan) S. Tilghman (Princeton, USA) R. Tjian (Berkeley, USA) M. Wigler (Cold Spring Harbor, USA)
Editors T. Grodzicker (Cold Spring Harbor) N. Hastie (Edinburgh)
Managing Editor J. Cuddihy (Cold Spring Harbor)
Editorial/Production N. Dumser, Technical Editor V. Nicolette, Production Editor L. Olsewski, Editorial Secretary
EDITORIAL OFFICES
United States Cold Spring Harbor Laboratory Press Box 100, 1 Bungtown Road Cold Spring Harbor, New York 11724-2203 Phone 516-367-8492 FAX 516-367-8532 e-mail genesdev^? cshl.org Web site http://www.cshl.org
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GENES & DEVELOPMENT (ISSN 0890-9369) is published semimonthly for $485 (institutional), $120 (individual making personal payment), $167 (Genet-ical Society of Great Britain members) by Cold Spring Harbor Laboratory Press, Bungtown Road, Cold Spring Harbor, New York 11724, in association with the Genetical Society of Great Britain. Second-class postage is paid at Cold Spring Harbor and additional mailing offices. POSTMASTER: Send address changes to Cold Spring Harbor Laboratory Press, POB 100, 1 Bungtown Rd., Cold Spring Harbor, New York 11724-2203. Subscription Price Orders may be sent to Cold Spring Harbor Laboratory Press, Fulfillment Department, 10 Skyline Drive, Plain view, NY 11803-9729. Telephone: Continental US except NY State, 1-800-843-4388) all other locations, 516-349-1930/1931/1932. FAX 516-349-1946. Volume 9, 1995, $485, U.S. institutional; $580, R.O.W. institutional. Personal subscription rate: $120, U.S.; $215, R.O.W. Price includes surface postage for U.S. and airlift for R.O.W. Genetical Society members, $167.00. All subscriptions are entered for the calendar year and must be prepaid. Personal subscriptions must be prepaid by personal check, credit card, or money order. All checks must be for US dollars and drawn on a US bank. Genetical Society members may also subscribe by check, payable to the Genetical Society, for $167 (includes airlift). Send to: Dr.
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Change of address Please enclose recent mailing label with address change; allow 4 weeks. Advertising To advertise in Genes &. Development, contact Deborah Duf ton, Advertising Manager, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York 11724-2203; telephone 516-367-8351. Photo Copy Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Cold Spring Harbor Laboratory Press for libraries and other users registered with the Copyright Clearance Center (CCC) Transactional Reporting Service, provided that the base fee of $5.00 per copy is paid directly to CCC, 21 Congress St., Salem, MA 01970 (0890-9369/95 $5.00 + 0.). This consent does not extend to other kinds of copying, such as copying for general distribution for advertising or promotional purposes, for creating new collective works, or for resale. Copyright © 1995 by Cold Spring Harbor Laboratory Press
Contents GENES & DEVELOPMENT October 1, 1995
Research papers Loss of methylation activates Xist in somatic but not in embryonic cells 2325 Caroline Beard, En Li, and Rudolf Jaenisch
Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates 2335 cellular DNA replication in suprabasal differentiated keratinocytes Shinta Cheng, Delf-Christian Schmidt-Grimminger, Thomas Murant, Thomas R. Broker, and Louise T. Chow
Conditional ectopic expression of C/EBPp in NIH-3T3 cells induces PPAR7 and 2350 stimulates adipogenesis Zhidan Wu, Yuhong Xie, Nancy L.R. Bucher, and Stephen R. Farmer
Mice lacking cyclin Dl are small and show defects in eye and mammary gland development 2364 Vera Fantl, Gordon Stamp, Anton Andrews, Ian Rosewell, and Clive Dickson
lethal of scute, a proneural gene, participates in the specification of muscle progenitors during 2373 Drosophila embryogenesis Ana Carmena, Michael Bate, and Fernando Jimenez
scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes 2384 neuronal development Margaret Roark, Mark A. Sturtevant, John Emery, Harald Vaessin, Ellsworth Grell, and Ethan Bier
Disassembly of the Mu transposase tetramer by the ClpX chaperone 2399 Igor Levchenko, Li Luo, and Tania A. Baker
Formation and resolution of double-strand break intermediates in V[D)J rearrangement 2409 Dale A. Ramsden and Martin Gellert
Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation 2421 in yeast Giordano Caponigro and Roy Parker
U14 base-pairs with 188 rRNA: a novel snoRNA interaction required for rRNA processing 2433 Wen-Qing Liang and Maurille J. Fournier
Cover Induction of host chromosomal DNA repUcation in the absence of cell division in terminally differentiated keratinocytes of a juvenile laryngeal papilloma caused by human papillomavirus type 6 infection. Autoradiographic signals from [^H]thymidine incorporation were visualized by darkfield microscopy in the superficial strata as well as in the cycling basal cells. (For details, see Cheng et al., p. 2335.)