Whole Effluent Toxicity Whole Effluent Toxicity and How It May Help Youand How It May Help You

&&One City’s Experience Evaluating One City’s Experience Evaluating

Unfamiliar WastestreamsUnfamiliar Wastestreams

Presented ByPresented ByLyle MilbyLyle Milby

City of NormanCity of NormanEnvironmental ServicesEnvironmental Services

August 5, 2009August 5, 2009Region VI Pretreatment WorkshopRegion VI Pretreatment Workshop

Whole Effluent Toxicity and Whole Effluent Toxicity and How It May Help YouHow It May Help You

BiomonitoringBiomonitoring Indicator of Unregulated Industrial ActivityIndicator of Unregulated Industrial Activity

Regulatory ToolRegulatory Tool Evaluate New Wastestreams & PollutantsEvaluate New Wastestreams & Pollutants

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 22

One City’s Experience One City’s Experience Evaluating Unfamiliar Evaluating Unfamiliar

WastestreamsWastestreams Tools for Evaluating Unfamiliar Tools for Evaluating Unfamiliar

Wastestreams & PollutantsWastestreams & Pollutants Norman’s Experience with WET & Norman’s Experience with WET &

Respirometric TestingRespirometric Testing Writing WET into a Control MechanismWriting WET into a Control Mechanism

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 33

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 44

City of Norman, OklahomaCity of Norman, Oklahoma

Medium Size City – 107,000 PopulationMedium Size City – 107,000 Population 7 SIUs & 2 CIUs7 SIUs & 2 CIUs Technology CorridorTechnology Corridor

University of OklahomaUniversity of Oklahoma Astellas Pharma TechnologiesAstellas Pharma Technologies SouthWest NanoTechnologiesSouthWest NanoTechnologies

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 55

Wastestream Evaluation Wastestream Evaluation ToolboxToolbox

EPA Regulations & Guidance DocumentsEPA Regulations & Guidance Documents

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 66

Wastestream Evaluation Wastestream Evaluation ToolboxToolbox

EPA Regulations & Guidance DocumentsEPA Regulations & Guidance Documents Material Safety Data Sheets - MSDSMaterial Safety Data Sheets - MSDS

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 77

Wastestream Evaluation Wastestream Evaluation ToolboxToolbox

EPA Regulations & Guidance DocumentsEPA Regulations & Guidance Documents Material Safety Data Sheets - MSDSMaterial Safety Data Sheets - MSDS World Wide WebWorld Wide Web

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 88

Wastestream Evaluation Wastestream Evaluation ToolboxToolbox

EPA Regulations & Guidance DocumentsEPA Regulations & Guidance Documents Material Safety Data Sheets - MSDSMaterial Safety Data Sheets - MSDS World Wide WebWorld Wide Web Yahoo Industrial Pretreatment ListserveYahoo Industrial Pretreatment Listserve

http://groups.yahoo.com/group/pretreatment_coordinators/http://groups.yahoo.com/group/pretreatment_coordinators/

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 99

Wastestream Evaluation Wastestream Evaluation ToolboxToolbox

EPA Regulations & Guidance DocumentsEPA Regulations & Guidance Documents Material Safety Data Sheets - MSDSMaterial Safety Data Sheets - MSDS World Wide WebWorld Wide Web Yahoo Industrial Pretreatment ListserveYahoo Industrial Pretreatment Listserve

http://groups.yahoo.com/group/pretreatment_coordinators/http://groups.yahoo.com/group/pretreatment_coordinators/

Toxicity TestingToxicity Testing WETWET Respirometric TestRespirometric Test

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1010

University of OklahomaUniversity of Oklahoma

Research LaboratoriesResearch Laboratories Power PlantPower Plant Printing ServicesPrinting Services Small Quantity Generator of Hazardous Small Quantity Generator of Hazardous

WasteWaste

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1111

Astellas Pharma Astellas Pharma TechnologiesTechnologies

Categorical Industrial User – CIUCategorical Industrial User – CIU 40 CFR Part 439 – Pharmaceutical 40 CFR Part 439 – Pharmaceutical

Manufacturing Point Source CategoryManufacturing Point Source Category• Subpart D – Mixing / Compounding and Subpart D – Mixing / Compounding and

Formulation SubcategoryFormulation Subcategory• Subpart E – Research SubcategorySubpart E – Research Subcategory

Regulating Waste From New Drug Regulating Waste From New Drug DevelopmentDevelopment

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1212

SouthWest SouthWest NanoTechnologies, Inc.NanoTechnologies, Inc.

Significant Industrial UserSignificant Industrial User 40 CFR Part 403 – General Pretreatment 40 CFR Part 403 – General Pretreatment

Regulations for Existing and New Sources of Regulations for Existing and New Sources of PollutionPollution

Local LimitsLocal Limits Manufacturer of Single-Walled Carbon Manufacturer of Single-Walled Carbon

Nanotubes - CNTsNanotubes - CNTs

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1313

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a CompoundToxicity of a Compound New Pharmaceutical CompoundNew Pharmaceutical Compound

Toxicity of a Total DischargeToxicity of a Total Discharge Acid Neutralization System with potential Acid Neutralization System with potential

NanowasteNanowaste

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1414

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a CompoundToxicity of a Compound AstellasAstellas

• Establish Toxicity LevelEstablish Toxicity Level Ceriodaphnia dubia (NOEC 3.125 mg/L)Ceriodaphnia dubia (NOEC 3.125 mg/L) Pimephales promelasPimephales promelas

daylbsday

gal

gal

L

Kg

lbs

g

Kg

mg

g

L

mg2.260

1

000,000,10

1

785.3

1

2.2

000,1

1

000,1

1

1

125.3

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1515

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a CompoundToxicity of a Compound AstellasAstellas

• Establish Toxicity LevelsEstablish Toxicity Levels Ceriodaphnia dubia (NOEC 3.125 mg/L)Ceriodaphnia dubia (NOEC 3.125 mg/L) Pimephales promelasPimephales promelas

Maximum granulation batch size 15KgPercent YM150 used in the product 41.5%Estimated loss to drain, maximum 5%

daylbsday

gal

gal

L

Kg

lbs

g

Kg

mg

g

L

mg2.260

1

000,000,10

1

785.3

1

2.2

000,1

1

000,1

1

1

125.3

daylbskglbsdayKg 68.02.2%5%5.4115

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1616

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a CompoundToxicity of a Compound AstellasAstellas

• Establish Toxicity LevelsEstablish Toxicity Levels Ceriodaphnia dubia (NOEC 3.125 mg/L)Ceriodaphnia dubia (NOEC 3.125 mg/L) Pimephales promelasPimephales promelas

• Evaluate Impact on Biological Treatment Evaluate Impact on Biological Treatment ProcessesProcesses

Respirometric Testing of Activated SludgeRespirometric Testing of Activated Sludge• 3mg/L, 2mg/L, and 1mg/L3mg/L, 2mg/L, and 1mg/L• No inhibition observedNo inhibition observed

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1717

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a Total DischargeToxicity of a Total Discharge SWeNTSWeNT

• Synthesize WastestreamSynthesize Wastestream• Determine Dilution RatioDetermine Dilution Ratio

%015.01000.10

015.0

MGD

MGD

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1818

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a Total DischargeToxicity of a Total Discharge SWeNTSWeNT

• Evaluate Impact on BiomonitoringEvaluate Impact on Biomonitoring WET Test MethodWET Test Method 0.5%, 1.0%, 5.0%, 32%, and 5.0% +CNT Dilutions0.5%, 1.0%, 5.0%, 32%, and 5.0% +CNT Dilutions

• Evaluate Impact on Biological Treatment Evaluate Impact on Biological Treatment ProcessesProcesses

Respirometric Testing of Activated SludgeRespirometric Testing of Activated Sludge 0.001%, 0.005%, and 0.023% Dilutions0.001%, 0.005%, and 0.023% Dilutions

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 1919

Steps in Making the Steps in Making the DeterminationDetermination

Toxicity of a Total DischargeToxicity of a Total Discharge SWeNTSWeNT

• Build Manufacturing Facility and Wastewater Build Manufacturing Facility and Wastewater Treatment SystemTreatment System

• Reevaluate Impact on BiomonitoringReevaluate Impact on Biomonitoring WET Test MethodWET Test Method 0.5%, 1.0%, 5.0%, and 32% Dilutions0.5%, 1.0%, 5.0%, and 32% Dilutions

• Reevaluate Impact on Biological Treatment Reevaluate Impact on Biological Treatment ProcessesProcesses

Respirometric Testing of Activated SludgeRespirometric Testing of Activated Sludge 0.5%, 1.0%, 5.0%, and 32% Dilutions0.5%, 1.0%, 5.0%, and 32% Dilutions

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 2020

City of NormanCity of NormanRoot Control ProgramRoot Control Program

Introduction of Biocides into the POTWIntroduction of Biocides into the POTW OPDES Requires Biomonitoring During OPDES Requires Biomonitoring During

Such EventsSuch Events Develop Control Document With WET Develop Control Document With WET

Testing LanguageTesting Language

August 5, 2009August 5, 2009 25th Annual Region VI Pretreatment Workshop25th Annual Region VI Pretreatment Workshop 2121

Questions?Questions?