white biotechnology with plant cells · subsequent to the fermentation the downstream processing...

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DRESDEN UNIVERITY OF TECHNOLOGY Institute of Food Science and Bioprocess Engineering Financial support: European Social funds and the Free State of Saxony Project number: 080938406 Project term: 01.10.2009 - 30.09.2012 Contact details: Dipl.-Ing. Felix Lenk fon: +49 351 / 463 36943 fax: +49 351 / 463 37761 e-mail: [email protected] White Biotechnology with Plant cells Development of a process for the production of plant active substances for the food, wood, pharmaceutical and cosmetics industry F. Lenk 1 , Ch. Haas 1 , K. Geipel 1 , S. Schulz 1 , A. Lippert 2 , J. Püschel 2 , J. Endrikat 3 , A.-K. Hüske 3 , H. Delenk 4 , T. Bley 1 , A. Wagenführ 4 , E. Günther 3 , J. Ludwig-Müller 4 & J. Steingroewer 1 1 Institute of Food Technology and Bioprocess Engineering, Dresden University of Technology, 01062 Dresden, Germany 2 Institute of Botany, Dresden University of Technology, 01062 Dresden, Germany 3 Chair of Business Administration, especially Environmental Mgmt. and Accounting, Dresden University of Technology, 01062 Dresden, Germany 4 Institute of Wood and Paper Technology, Dresden University of Technology, 01062 Dresden, Germany Application Food additives (E162 & E307) Fungicides (natural wood preservatives) Final product after downstreaming α-Tocopherol Triterpenes (e.g. oleanolic acid) Up-scale Processing in bioreactor systems Analytics Induction & Cultivation Callus cultures Hairy roots Raw material Sunflower (α-Tocopherol) Salvia (Triterpenes) Rootbeet (red dye Betanin) Modeling and Simulation Ecological & economical assessment, eco-balance, hurdle analysis Selected model organisms produce scientific and economical relevant active agents and additives as secondary metabolites. Generation of undifferentiated cell-tissues (callus) with hormone addition as well as Hairy root organ complexes with the help of Agrobacterium rhizogenes. Subsequent to the fermentation the downstream processing and extraction of the desired target products from the bulk volume takes place. Usage of these ultra-pure, sustainable and with constant quality produced extracts as preservatives, dye and active additive in food (e.g. vitamine E) etc.. Transfer of Callus- and Hairy root cultures in a parallel bioreactor system for experimental determi- nation of optimal cultivation conditions in pilot plant station scale. O OH O H H H CH 3 C H 3 CH 3 C H 3 O OH O H H H CH 3 C H 3 C H 3 CH 3 oleanolic acid (OA) Ursolic acid (UA) HPLC-Chromatogramm of Salvia-callus extracts During the screen- ing of the cell lines the concentration of the active agents is determined with HPLC and GC-MS. (UA) (OA) RAMOS ® Structured growth models for simulation and visualization of growth morphology and distribution of secondary meta- bolites in Hairy root organ complexes. Individual-based Models for the growth of plant cell and tissue cultures. Analytical optimization of process control and design of bioreactors for plant cell and tissue cultures. MULTIFORS bioreactor system Analysis of environ- mental effects of the industrial application of biotechnological processes (Life Cycle Assessment). Innovation-barrier re- search with expert interviews included in a Delphi-study. Analysis of the economical potential of White Biotech- nology in Germany based on a periphery and market analysis. Ecological validation and optimization with respect to flow patterns of materials and component substances. untreated and treated wood sample

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  • DR

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    Insti

    tute

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    Fo

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    Scie

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    Financial support:

    European Social funds and the Free State of Saxony

    Project number: 080938406

    Project term: 01.10.2009 - 30.09.2012

    Contact details:

    Dipl.-Ing. Felix Lenk

    fon: +49 351 / 463 36943

    fax: +49 351 / 463 37761

    e-mail: [email protected]

    White Biotechnology with Plant cells Development of a process for the production of plant active substances for the

    food, wood, pharmaceutical and cosmetics industry

    F. Lenk1, Ch. Haas1, K. Geipel1, S. Schulz1, A. Lippert2, J. Püschel2, J. Endrikat3, A.-K. Hüske3, H. Delenk4,

    T. Bley1, A. Wagenführ4, E. Günther3, J. Ludwig-Müller4 & J. Steingroewer1

    1 Institute of Food Technology and Bioprocess Engineering, Dresden University of Technology, 01062 Dresden, Germany 2 Institute of Botany, Dresden University of Technology, 01062 Dresden, Germany

    3 Chair of Business Administration, especially Environmental Mgmt. and Accounting, Dresden University of Technology, 01062 Dresden, Germany 4 Institute of Wood and Paper Technology, Dresden University of Technology, 01062 Dresden, Germany

    Application

    Food additives

    (E162 & E307)

    Fungicides (natural wood preservatives)

    Final product after downstreaming

    α-Tocopherol Triterpenes (e.g. oleanolic acid)

    Up-scale Processing in bioreactor systems

    Analytics

    Induction & Cultivation

    Callus cultures Hairy roots

    Raw material

    Sunflower

    (α-Tocopherol)

    Salvia (Triterpenes)

    Rootbeet (red dye Betanin)

    M

    od

    elin

    g a

    nd

    Sim

    ula

    tion

    E

    colo

    gic

    al &

    eco

    no

    mic

    al a

    ssessm

    ent,

    eco-b

    ala

    nce

    , hu

    rdle

    analy

    sis

    Selected model organisms produce scientific and economical relevant active agents and additives as secondary metabolites.

    Generation of undifferentiated cell-tissues (callus) with

    hormone addition as well as Hairy root organ complexes

    with the help of Agrobacterium rhizogenes.

    Subsequent to the fermentation the downstream processing and extraction of the desired target products from the bulk volume takes place.

    Usage of these ultra-pure, sustainable and with

    constant quality produced extracts as preservatives,

    dye and active additive in food (e.g. vitamine E) etc..

    Transfer of Callus- and

    Hairy root cultures in a

    parallel bioreactor system

    for experimental determi-

    nation of optimal cultivation

    conditions in pilot plant

    station scale.

    O

    OH

    OH

    H

    H

    CH3CH3

    CH3CH3

    O

    OH

    OH

    H

    H

    CH3CH3

    CH3 CH3

    oleanolic acid (OA)

    Ursolic acid (UA)

    HPLC-Chromatogramm of Salvia-callus extracts

    During the screen-

    ing of the cell lines

    the concentration

    of the active agents

    is determined with

    HPLC and GC-MS.

    (UA)

    (OA)

    RAMOS®

    Structured growth

    models for simulation

    and visualization of

    growth morphology

    and distribution of

    secondary meta-

    bolites in Hairy root

    organ complexes.

    Individual-based

    Models for the

    growth of plant cell

    and tissue cultures.

    Analytical optimization

    of process control and

    design of bioreactors

    for plant cell and

    tissue cultures.

    MULTIFORS bioreactor system

    Analysis of environ-

    mental effects of the

    industrial application

    of biotechnological

    processes (Life Cycle

    Assessment).

    Innovation-barrier re-

    search with expert

    interviews included in

    a Delphi-study.

    Analysis of the

    economical potential

    of White Biotech-

    nology in Germany

    based on a periphery

    and market analysis.

    Ecological validation

    and optimization with

    respect to flow

    patterns of materials

    and component

    substances.

    untreated and treated wood sample