what separates you from everyone else€¦ · extract from hoodia gordonii interrogation of...

WHAT SEPARAT ES YOU FROM EV ERYONE ELSE

[ ACQUITY UPLC I-CLASS SYSTEM ]

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The ACQUITY UPLC® I-Class provides

the most powerful solution to the most

critical needs in separation science

today – successfully analyzing

compounds that are limited in amount

or availability amid a complex matrix,

more rapidly than ever before.

The ACQUITY UPLC story began in 2004,

when Waters introduced UPLC® Technology

to the world. Since then, the ACQUITY

UPLC family of systems has moved the

entire analytical industry forward.

The newest member of the ACQUITY

UPLC family, the ACQUITY UPLC I-Class

System, represents the next advancement

in liquid chromatography.

With UPLC, laboratories like yours

have conquered workflow bottlenecks,

improved the quality of information from

assays, and reduced solvent use. UPLC

fundamentally makes laboratories more

efficient and environmentally sound,

while making science-driven

organizations more effective.

Scientists are looking for answers to their

most complex challenges. The ACQUITY

UPLC I-Class System provides the

unmatched resolution and peak capacity

necessary to achieve more meaningful

information from chromatographic

separations – the very information

that drives critical decisions in any

science-based organization.

Simply put, the ACQUITY UPLC I-Class

is the highest performing LC instrument

available today.

Separation of a complex sample: Extract from Hoodia gordonii

Interrogation of information-rich samples requires a UPLC system that provides the highest quality information. The separation was achieved using the ACQUITY UPLC I-Class with a Xevo® G2 QTof and a 1.8-µm ACQUITY UPLC HSS T3 2.1 x 100 mm Column.

Extract of Hoodia gordonii was analyzed in collaboration with Ikhlas A. Khan, Ph.D., and Bharathi Avula, Ph.D., from the National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences at The University of Mississippi.

complex challengesYour most

now solved by the world’s best separation technology.


Never miss a detail or an opportunity. ACQUITY UPLC I-Class System Optimized UHPLC System

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ACQUITY UPLC I-Class System Optimized UHPLC system

UPLC vs. UHPLC: A clear difference

UPLC separations are defined by the use of sub-2-µm particle column packing materials that reduce dispersion during the separation. When total dispersion from the column and the system together is minimized, resolution and sensitivity are maximized. To realize the full benefits of sub-2-µm columns, the LC instrument must be optimized to preserve the high-efficiency separation.

In this example, the impurity analysis of tetracaine was run under the same conditions on the ACQUITY UPLC I-Class System (system dispersion of 5.5 µL) and a UHPLC system (system dispersion of 20 µL). Although the UHPLC system was optimized for lowest possible dispersion and system volume, the impact of system dispersion is clearly evident. Significantly better resolution was achieved with the ACQUITY UPLC I-Class System.


Because the details always matter.

Everyday, each of us relies on the outcome of analytical science in the development of critical medicines or food safety assessment. Now, with

the powerful separation capabilities of the ACQUITY UPLC I-Class System, new opportunities to improve our lives await your next discovery.

■■ Inhaled therapeutics and biotherapeutics

New combinations of

medicines and dosing

levels pose additional

analytical challenges

in terms of data

and sensitivity.

■■ High potency medications

For drugs that target

specific disease states

with reduced systemic

impact; you have new

abilities to analyze

potent therapeutics

designed for efficacy at

extremely low levels.

■■ Food safety

For an industry

with increasingly

stringent regulatory

controls backed by a

knowledgeable consumer

population with higher

standards; detect the

smallest amounts of

contaminants to ensure

products that sustain our

lives are unadulterated,

consistent, and safe.

■■ Purity and quality

Gain improved

capabilities in

profiling, detecting,

and quantifying

active substances

and impurities in raw

materials and finished

products. Ensure our

health and wellness,

whether you work

with food, beverages,

supplements, or drugs.


Confidence in results.Confidence in results that are built on more information

The ACQUITY UPLC I-Class System is designed to produce the

most accurate, reproducible separations. You will get the most

information possible for ultimate laboratory performance.

■■ Decreased dispersion for the highest resolution

■■ Rapid injection cycles and sample throughput

■■ Dramatically reduced carryover

Which in turn delivers: ■ Superior peak capacity ■ Highest sensitivity ■ Faster results ■ Better data


Next-generation column compartments

Column compartments for the ACQUITY UPLC

I-Class System are designed for system-to-system

equivalence as well as to provide versatility,

flexibility, and practicality for the chemistry and

conditions your analysis requires. Our low-volume

Column Heaters and Managers offer best-in-class

dispersion and extended peak capacity.

■■ Take advantage of two-

column flexibility, with

independent thermal zones

for each column. With an

extended temperature

range of 4 to 90 °C, the

system enables you to

change between methods

and columns, and enables

unattended operation when

throughput is critical.

■■ The Column Manager

can be configured with

interchangeable valves for

serial column runs with a

second ACQUITY UPLC

pump to support all the

functionality and flexibility

of 2D technology.

■■ Active solvent

pre-heating with unique

low-volume tubing:

Combines the lowest

possible dispersion

with consistent thermal

performance for accurate,

repeatable results.

Promotes accurate thermal

fidelity, resulting in uniform

efficiency system to system.

Whenever a method is

transferred between an

ACQUITY UPLC I-Class

or an ACQUITY UPLC

H-Class system, the

relative retention times

(or chromatographic

fidelity of the separation)

are preserved.


next generationDiscover the The scientific discoveries

with the biggest potential to

improve our lives often rely

on samples and compounds

that are the most difficult to

understand. The ACQUITY

UPLC I-Class System was

designed specifically for the

rapid pace of laboratories on

the cutting edge of research.

The ACQUITY UPLC I-Class

System tackles challenging

analyses that require

maximizing resolution of

sub-2-µm particles while

minimizing extra-column

effects. Minimal sample

carryover provides greater

confidence in results.

Reduced dispersion and

small peak volumes extend

the sensitivity of any

mass spectrometer.

With the ability to optimize

high throughput analyses,

you’ll find a more accurate

answer rapidly, without

compromising data quality.

You’ll reduce the risk of not

finding your compound of

interest or its impurities at

the lowest possible levels.

The system enables you to

take on the most demanding

separations with utmost

confidence in your results.

Finally – you have the

flexibility and resolving

power to measure and

analyze extremely

small quantities from

complex matrices.

Liquid chromatography’s theoretical maximum

As the flow rate in a gradient separation is increased, peak capacity achieves a theoretical maximum. The ACQUITY UPLC I-Class System allows you to approach the theoretical peak capacity for your separation by providing the necessary pressure range.

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Peak capacity = 271Flow rate = 1.8 mL/min

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Better results for your science. And your business.

Like the ACQUITY UPLC and

the ACQUITY UPLC H-Class,

the ACQUITY UPLC I-Class

uses dramatically less

solvent than standard LC

instruments – which means

less solvent to buy, and

less solvent to dispose.

So your laboratory is

saving money and helping

the environment.

Go to www.waters.com/green to calculate your solvent and environmental savings with ACQUITY UPLC.

of discovery.

http://www.waters.com/green

Power for ballistic gradients

The low dispersion characteristics of the ACQUITY UPLC I-Class System allow you to push your separations to a new level. This ballistic gradient for a series of anesthetics achieved standard deviations for retention time as low as 12 ms for six replicates. The separation achieved a peak capacity of 46 in 10 s with a 1/2 height peak width as low as 88 ms.

■■ There’s more to UPLC than high pressure

The industry-leading performance of an ACQUITY UPLC I-Class

System is made possible by purposeful engineering of its binary

solvent manager. This maximizes the utility of high pressures under the

stringent conditions required to achieve an optimal chromatographic

separation, while maintaining robustness and reproducibility.

Our holistic system design approach delivers the best resolution

and throughput, and is ideal for ballistic gradients and other fast,

challenging separations.

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System: ACQUITY UPLC I-Class (SM-FL)Column: 2.1 x 30 mm BEH C18 1.7 µmTemperature: 90 °CFlow rate: 2.0 mL/minMobile phase A: 10 mM ammonium bicarbonate pH 10Mobile phase B: AcetonitrileGradient: 35% to 95% B over 10 s (2 s pre-inject delay)Data rate: 80 Hz

Be Assured. Choose Waters Global Services.

■■ Waters Global Services helps

customers optimize laboratory

operations by providing superior

service, support, upgrades,

training, and Waters Quality

Parts.® For more information, go

to www.waters.com/services.

■■ Connections INSIGHT® Remote

Services provide secure,

web-based system monitoring,

instant alert notification, and a

direct link to Waters technical

experts. These services

help you maximize instrument

uptime, increase laboratory

productivity, and anticipate

system support requirements.


http://www.waters.com/services

Precision for reproducible injections

The ACQUITY UPLC I-Class System (SM-FTN) delivers precise, accurate injection volumes from 0.1 µL to 1.0 mL. For this series of anesthetics analyses, the injection linearity was assessed from 0.1 µL to 2.0 µL with six replicates at each level. Excellent linearity, repeatability, and accuracy were achieved across the injection volume range.

■■ Informatics solutions that adapt to your lab’s needs

The ACQUITY UPLC I-Class System is fully

compatible with Empower™ Software, our industry-

leading chromatography data software platform, as

well as MassLynx™ Software and its suite of data

processing Application Managers. Waters’ unique

eCord™ Technology records the usage and history

of your ACQUITY UPLC Columns and is part of the

ACQUITY UPLC I-Class console software.

■■ The most sensitive line of UPLC detectors available

The efficient separations achieved by the ACQUITY

UPLC I-Class are enabled by new ultra-low

dispersion absorbance detector flow cells,

delivering high sensitivity and precisely matching

peak volume to flow cell volume to maintain peak

integrity. Only Waters can offer the widest range

of UPLC-optimized optical and mass detectors on

the market, delivering the highest sensitivity and

selectivity for your applications.

■■ Flexible and precise sampling

Depending on your application needs, choose

either the highest precision and lowest dispersion

fixed-loop Sample Manager (SM-FL), or the

world’s first low-dispersion Sample Manager wtih

flow-through needle (SM-FTN), which delivers

high-precision injections with excellent sample

recovery and wide linear dynamic range. For mass

spectrometry, take advantage of a wide range of

injection volumes from 0.1 to as high as 1000 µL.

Benzocaine R2 = 0.99996 Procaine R2 = 0.99995

Tetracaine R2 = 0.99992

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ACQUITY UPLC CommunityLearn, share, and advance your chromatography

skills. Once you invest in an ACQUITY UPLC System,

you’ll be able to join our exclusive community

and connect with thousands of your peers.

www.waters.com/myuplc

The ACQUITY UPLC

I-Class System is built

to dramatically reduce

chromatographic dispersion

for better resolution than

you’ve ever experienced.

After listening to scientists

around the world, we created

the only system that delivers

the carryover performance

necessary to match the broad

dynamic range demanded

by the most sensitive

MS applications.

Whether paired with a

high performance Waters

mass spectrometer or

with a non-Waters MS, the

ACQUITY UPLC I-Class

System delivers unrivaled

sensitivity – so you’ll never

miss a single detail.

■■ Achieve ultimate

performance, and see

better results faster.

■■ Increased resolution

and reduced dispersion

deliver greater

selectivity and

enhanced sensitivity.

■■ Lower carryover and

higher throughput

combine as the

superior inlet to MS.

■■ Minimal system-to-

system variability.

■■ Get more information

from your mass

spectrometer, faster.

See what others never have.

With the ACQUITY UPLC I-Class System and the Xevo TQ-S,

your laboratories will thrive with the definitive platform for

high throughput, high sensitivity quantitative analyses.


When you combine an ACQUITY UPLC I-Class System with an advanced mass spectrometer,

you have an engine powerful enough to pull an entire laboratory forward and to see what

other LC technologies cannot show you.

Compound name: OmeprazoleCorrelation coefficient: r = 0.999985, r2 = 0.999970Calibration curve: 134101 * x + -12.3789Response type: External Std, AreaCurve type: LinearOrigin: ExcludeWeighting: 1/xAxis trans: None

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MRM of 1 Channel ES+346.083 > 198.068 (Omeprazole)

7.06e7

MRM of 1 Channel ES+346.083 > 198.068 (Omeprazole)

4.42e3

Omeprazole 10 ng 0403

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...Even to greater than four orders of magnitude.

With carryover minimized for the assay of omeprazole, the calibration range on the ACQUITY UPLC I-Class System coupled with the Xevo TQ-S was determined to be 500 ag to 10 pg on-column with a correlation coef-ficient of 0.99997 across greater than four orders of magnitude.

Designed for mass spectrometry...

The ACQUITY UPLC I-Class was paired with a Xevo TQ-S for the analysis of omeprazole, achieving a quantification range of 500 ag to 10 pg on-column. No detectable carryover within 20% of the limit of quantitation was observed after the injection of the highest concentration standard.

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Korea 82 2 6300 4800

Waters, UPLC, ACQUITY UPLC, Connections INSIGHT, Xevo, and Waters Quality Parts are registered trademarks of Waters Corporation. T he Science of What’s Possible, eCord, Empower, and MassLynx are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2011 Waters Corporation. Produced in the U.S.A. May 2011 720003920EN LB-PDF

www.waters.com/iclass

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what separates you from everyone else€¦ · extract from hoodia gordonii interrogation of...

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