what do these have in common? hiv infection transplanted organs communication between neurons drug...
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What do these have in common?
• HIV infection
• Transplanted organs
• Communication between neurons
• Drug addiction
• Cystic fibrosis
• hypercholesteremia
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• selectively permeable
• hold teams of enzymes
Membranes organize the chemical activities of cells
Cytoplasm
Figure 5.10
Plasma membrane
• Contact between cell and environment
• Keeps useful materials inside and harmful stuff outside
• Allows transport, communication in both directions
• Anchors energy-converting enzymes
Plasma membrane components
Phospholipid bilayer
Cholesterol
Proteins
Glycocalyx
polarhead
nonpolartails
P –
Phospholipid bilayer
hydrophobic molecules hydrophilic molecules cytosol
THE PLASMA MEMBRANE
phospholipids cholesterol
cytoskeletonperipheralprotein
integralprotein
Cholesterol blocks some small molecules, adds fluidity
• Membrane Proteins– span entire membrane or lie on
either side– Purposes
• Structural Support• Recognition• Communication• Transport
• Glycocalyx
– Composed of sugars protruding from lipids and proteins
– Functions
• Binding sites for proteins
• Lubricate cells.
• Stick cells down.
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Many membrane proteins are enzymes
Figure 5.13
• Some proteins function as receptors for chemical messages from other cells
– The binding of a messenger to a receptor may trigger signal transduction
Enzyme activity Signal transduction
Messenger molecule
Receptor
Activated molecule
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• The plasma membrane of an animal cell
Fibers of the extracellular matrix
Figure 5.12
Glycoprotein Carbohydrate (of glycoprotein)
Microfilaments of the cytoskeleton
Phospholipid
Cholesterol
Proteins
CYTOPLASM
Glycolipid
• Diffusion and Gradients
– Diffusion = movement of molecules from region of higher to lower concentration.
– Osmosis = diffusion of water across a membrane
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• In passive transport, substances diffuse through membranes without work by the cell
EQUILIBRIUMMolecule of dye
Figure 5.14A & B
Membrane
EQUILIBRIUM
free water molecule: can fit through pore
bound water moleculesclustered around sugar:cannot fit through pore
pore
sugar
H2O
bagbursts
selectively permeable membrane
water molecule
pure water
sugar molecule
(a)
selectively permeable membrane
(b)
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• water travels from an area of higher concentration to an area of lower water concentration
Osmosis = diffusion of water across a membraneHypotonicsolution
Figure 5.15
Solutemolecule
HYPOTONIC SOLUTION
Hypertonic solution
Selectivelypermeablemembrane
HYPERTONIC SOLUTION
Selectivelypermeablemembrane
NET FLOW OF WATER
Solute molecule with cluster of water molecules
Water molecule
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Osmosis causes cells to shrink in a hypertonic solution and swell in a hypotonic solution
Water balance between cells and their surroundings is crucial to organisms
osmoregulation = control of water balance
isotonic solution hypertonic solution hypotonic solution
10 microns
equal movement of waterinto and out of cells
net water movement out of cells
net water movement into cells
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Small nonpolar molecules - simple diffusion
• Many molecules pass through protein pores by facilitated diffusion
Passive transport = diffusion across membranes
Figure 5.17
Solutemolecule
Transportprotein
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• transport proteins needed
• against a concentration gradient
• requires energy (ATP)
Active transport
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Active transport in two solutes across a membrane
• Na+/K+ pump
• Protein shape changeFigure 5.18
Transportprotein
1
FLUIDOUTSIDECELL
Firstsolute
First solute, inside cell, binds to protein
Phosphorylated transport protein
2 ATP transfers phosphate to protein
3 Protein releases solute outside cell
4 Second solute binds to protein
Second solute
5 Phosphate detaches from protein
6 Protein releases second solute into cell
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
exocytosis = vesicle fuses with the membrane and expels its contents
Exocytosis and endocytosis transport large molecules
Figure 5.19A
FLUID OUTSIDE CELL
CYTOPLASM
b
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
– or the membrane may fold inward, trapping material from the outside (endocytosis)
Figure 5.19B
food particle
particle enclosed in vesicle
phagocytosis
1 32
Phagocytosis, “cell eating”
—How the human immune system ingests whole bacteria or one-celled creatures eat.
(cytoplasm)
vesicle containing extracellular fluid
pinocytosis
2
extracellular fluid
plasma membrane
vesiclecytosol
receptors captured molecules
coatedpit
vesicle
bacterium pseudopodium
vesicle
Receptor-mediated endocytosis
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Cholesterol can accumulate in the blood if membranes lack cholesterol receptors
Figure 5.20
LDL PARTICLEPhospholipid outer layer
Protein
Cholesterol
Plasma membraneCYTOPLASM
Receptor protein
Vesicle
What do these have in common?
• HIV infection
• Transplanted organs
• Communication between neurons
• Drug addiction
• Cystic fibrosis
• hypercholesteremia