SummaryThe Summary section should have a short statement of the background, justification for analysis by light scattering or other tech-niques supported by Wyatt, and the key takeaways of the app note or white paper. Two-three paragraphs is usually enough. The primary goal of the summary is to en-tice the readers to read the rest, or to un-derstand that it does not really hold any-thing of interest and to move on to the next app note.In some instances you might want to in-clude a copy of a key data graph in the summary. It can have a caption, but if a du-plicate appears later, do not number it.

IntroductionIntroduce the subject of the application note, the importance to the world, and the challenges such as where other characteri-zation techniques fail or the importance of high-throughput, etc. Break the text up into paragraphs gener-ously, it makes it easier to read. Don’t skimp on graphics, graphs of data and ta-bles of results. Most Figures should fit into a column but as shown below, you can also have them spread across the page in a sin-gle-column section between two-column sections Subsection 1The first instance of each product such as DAWN®, miniDAWN® or µDAWN®, should have the registered trademark or as ap-propriate, and be linked to the web site us-ing the respective URL mnemonic shortcut. The list of shortcuts is in the Support Center

under https://www.wyatt.com/files/litera-ture/sales/Wyatt-web-shortcuts.docx. Tech-niques such as SEC-MALS, DLS, etc should also be referenced to the web site, and where appropriate other pages that have shortcuts such as Protein Applications, webi-nars, the Bibliography or theory pages.

In the introduction you might include one or two figures that do not have numbered cap-tions. Some examples are a decorative graphic, an image of the instrument, a chemical representation or a ribbon repre-sentation of the protein being studied. Link references to figures and tables appro-priately. An example is presented in .

TNXXXX: MALS Instrument Maintenance

Figure 1. Do not use screen shots from the software or copied graphs unless you can make the labels, axis titles and legend items clearly legible and uniform across the application note. It is better to export to Excel and create uniform size and styling.

Another way to achieve quality graphs is to copy and paste into Powerpoint, size appro-priately, retype the axis labels, titles, and legends in text boxes, add any callouts or arrows, and crop the graph to remove the sections that were retyped. Then copy the whole thing and paste as a picture into the Word doc.

Materials and MethodsDescribe in brief the analyte, instrument setup and procedures. This will help the reader understand what it’s about and how easy it is to do. Don’t forget to mention the Wyatt software used to collect and analyze data, with correct capitalization, trademark, and link: ASTRA®, DYNAMICS®, CALYPSO®, SCOUT DPS®, etc.Subsection Phasellus eu ornare nulla, quis scelerisque mi. Quisque consequat tellus leo, eget effici-tur leo iaculis et. Lorem ipsum 1 dolor sit ame.

Figure 2. Graphs should be centered on the column, and where ap-propriate, fill the column width. They can be sized and placed judi-ciously to optimize text flow.

SubsectionIn aliquet nulla neque, a ultricies justo dapibus ac. Nullam vel tempus augue, vel fringilla augue. Mauris lacus arcu, dapibus nec justo hendrerit, laoreet pharetra elit. In egestas imperdiet diam, sed placerat dui dictum et. Aliquam ligula dui, tempus et porta sed, iaculis semper neque. Integer semper, metus in pulvinar venenatis, mau-ris ante posuere mi, eu euismod orci tellus in ante. Fusce porta libero eu blandit ultri-ces. Praesent convallisnibh elit. Etiam euis-mod lobortis augue, non laoreet lorem po-suere nec.

Results and DiscussionInclude key results, with graphs and tables, and a description of the information present in each. Remember that the reader may not be familiar with the subject matter or instru-mentation, but we still want the application note to appear meaningful. Can you make these results relate to other areas of re-search and characterization by wording the text in a broadly applicable manner? Can you cater to both the experts – with specific results – and those who may not be from this field with more general statements?Don’t just say ‘here, we made a measure-ment’. What have you learned from the analysis? How did it benefit science and hu-manity? What did our instruments contrib-ute that would not otherwise have been

possible, whether in quality, quantity, time, effort, etc? What other Wyatt techniques could be applied to flesh out the under-standing?One angle: Ut id arcu ullamcorper, efficitur neque nec, tempor massa. Fusce lacus mau-ris.Another angle: Sed scelerisque quam non mi finibus rutrum sit amet in justo. Proin eleifend est non venenatis rutrum. Quisque metus felis, commodo ac volutpat et, blan-dit sit amet elit. Suspendisse eu neque eget lorem pulvinar ornare at malesuada dolor.Sub-sub-sectionIn aliquet nulla neque, a ultricies justo dapibus ac. Nullam vel tempus augue, vel

fringilla augue. Mauris lacus arcu, dapibus nec justo hendrerit, laoreet pharetra elit. In egestas imperdiet diam, sed placerat dui dictum et.

Laser (nm)

Interference Filters (20 nm)

Sample 1App. MW (kDa)

Sample 2App. MW (kDa)

665 No 89 ± 6 176 ± 6

665 Yes 30 ± 5 34.4 ± 5

786 No 30.7 ± 0.3 24.4 ± 0.1

786 Yes 27.2 ± 0.3 14.2 ± 0.1

Table 1. Some results are best presented this way.

Figure 3. Some figures or tables will not work well in a single column, so insert a section with a single column. Displaying the formatting marks (File->Options->Display->Show all formatting marks) helps understand the sections. This: ====== means a new section. The document contin-ues below.

In aliquet nulla neque, a ultricies justo dapibus ac. Nullam vel tempus augue, vel fringilla augue. Mauris lacus arcu, dapibus nec justo hendrerit, laoreet pharetra elit. In egestas imperdiet diam, sed placerat dui dictum et.

ConclusionsMention both the overall relevance of the analy-sis to science, industry, humanity, etc. and the key takeaways regarding the benefits of light scattering and other Wyatt instruments/tech-niques.

Sometimes the layout looks best if you move a new section to the top of the next column by in-serting a Column break, from Layout->Breaks->Column.

References If no references, delete this section.

1. Mika, M. and N. Marianne, Utilization of Membrane Processes in Treating Various Effluents Generated in Pulp and Paper In-

dustry, in Handbook of Membrane Sepa-rations 2008, CRC Press. p. 981-1006.

2. Albinsson, B., et al., The origin of lignin fluorescence. J. Mol. Struct., 1999. 508(1–3): p. 19- 27.

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