Advanced Genetics Lecture 5: Prokaryotic and Viral Genomes and Recombinant DNA Technology (Part 1)

A. Prokaryotic Genomesa. General Concepts

b. Restriction Enzymes

i. “Blunt End” vs. “Sticky End”

http://bio305lab.wdfiles.com/local--files/2012project:enzymes/rest_enz.jpeg

c. Plasmids as Vectors

B. Viral Genomesa. General Concepts

C. Viral Replication: General Concepts

https://www.immunology.org/sites/default/files/Virus-replication-figure-1.jpg

D. Viruses as Vectorsa. Adeno-associated Virus Replication

b. Adenoviruses, Lentiviruses and Adeno-associated Viruses

E. Studying Gene Activitya. Microarrays

b. RNA Seq and Metatranscriptomics

https://en.wikipedia.org/wiki/File:Summary_of_RNA-Seq.svg

F. Interfering with Gene Activitya. Gene Knockouts

b. RNA interference Video: http://www.nature.com/nrg/multimedia/rnai/animation/index.html?foxtrotcallback=true