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We have viruses!! Tucson High School Biotechnology Course Spring 2010

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Results from Last Class Synechococcus Prochlorococcus

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Results from Last Class: Controls Negative Control Positive Control 10 4 dilution (higher concentration of viruses) Positive Control 10 6 dilution (lower concentration of viruses

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Results from Last Class: Tara Oceans Samples No Plaques: 11 Tara Samples Plaques!! 6 Tara Samples

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Results from Tara Oceans

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Now What?? Pick Plaques! Yes! No! 3 l

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Whats Next? Generate many more viruses We need a lot! Each virus: ~55 ag DNA (0.000000000000000055 g) We need: a few g (0.000001 g) => 1.8 x 10 10 viruses (18000000000 viruses)

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A reminder Overlays are NOT solid! **Do NOT tilt, shake, jostle, or turn over your plates!**