water structures carrying dna information, application to hiv/aids and autism l.montagnier,...
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Water structures carrying DNA information,
Application to HIV/AIDS and Autism
L.Montagnier, Borovets, October 23, 2013
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The Evolution of Medicine
Toward 4 P Qualities
• P1 Preventive
• P2 Predictive
• P3 Personalized
• P4 Participative
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Emergence or reemergence of new epidemics due to :
Globalization of exchanges and travels Demography: concentration in large cities Nutrition (pesticides, water) Environmental factors Climate changes, electromagnetic radiations Contacts with wild and farm animals Decline of immune defenses
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- Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common:
oxidative stressinfectious agents (?)
- Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common:
oxidative stressinfectious agents (?)
The most important burdenChronic Diseases
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Radiations (, X, UV, visible, hertzian)
Air chemical pollution
Inadequate Food
Excessive physical exercize
tobacco smoke
alcohol
ischemia
Parasitic infections
Bacterial infections
Viral infections
Various environmental factors effects accumulate Various environmental factors effects accumulate
+++++
++
+
= DISEASE+
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Lipid hyperoxydation (plasma, cell membranes)
Protein Oxydation --> destruction, agregates
DNA oxydation --> Mutations, Chromosomal
Breakage
Pathological effects of Oxydative Stress
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Oxidative stressWeakens the immune system
Activates transcription factors(NF-kappa B)
Activates genes involved in cell division, inflammatory cytokines,
lymphocytes activation
Immune dysfunction, apoptosis(TH1 TH2)
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The Human Genome
Genes (+ introns +promoters) 3%Retroelements: retroviruses 8%
retrotransposons 13%LTR 23%Total 47%
Repeats 50%LINE 22%SINE 13%
Unknown ?
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Mucosa
Skin
Mucosa
Skin
Our microbial ecosystem :
Constant exposure to microbial agents and immune protection
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Extreme genetic plasticityof microorganisms:
virus : HIV, Influenza Bacteria (plasmids) Parasites (retrotransposons)
against reaction of the immune system
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- Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes
- Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes
How bacteria have learned how to persist despite the immune system
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Parasite Bacterium Virus
(Worm) intracellular
Filarial nematode Wolbachia
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Persistent cold infections
Antibiotics affect them only when they go out of sanctuaries
•Inability of the immune system to eradicate them (tolerance, oxidative stress)•Non-multiplicative forms of « classical » bacteria•intracellular bacteria •Sanctuaries(bone marrow, joints, intestine, brain, ….)•Vectors (Parasites) •Remote effects (toxin, nanostructures)
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EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASESA physical and molecular approach
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Two technologies for detecting bacteria and viral DNA’s
•A new technology based on the production of electromagnetic waves
•The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability
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oligo
oligo
Taqpolymerase
Classical model of PCR
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Resonance emission of low frequency electromagnetic waves by high water dilutions
of DNA.
A newly discovered property of DNA :
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Molecular recognition
without close contact(by waves and resonance)
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Capture of the signals
Amplifier Computer
X 500 Signal Analysis software
Sensor coil
Sample
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Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.
Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.
FACTS
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2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16
Filtration 0.1
0.02
7-100 Hz
1000 -3000 Hz
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Time (sec)
Am
plitu
de
Noise
(+)
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Spectral Frequency Analysis
Fourier Tranformation
A positive signal is defined by:
amplitude increaseShift to higher frequencies (500-2000 Hertz)
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Noise
(+)
Frequency (1-20000 Hertz)
Tim
e (s
ec)
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Suspensions of pure culture of bacteria with pathogenic potential are producing electromagnetic signals (EMS) in the range of dilution usually 10-8 10-13, sometime (E.coli) up to 10-18
They are: E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.
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Micro-organisms involved in EMS induction
1.DNA from main pathogenic bacteriaStreptococcusStaphylococcusPseudomonasMycoplasma pirumSalmonellaClostridiumProteus mirabilisB. SubtilisBorrelia burgdorferiSutterella
- From viruses HIV1Influenza group AHBVHCV
- Genes involved M.pirum adhesinHIV genes
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Free polymerself maintained
by EMS
Water excitated statecrystal like gel
no EMS
10-2 10-9
DNA
Interpretation
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I – DNA’s emit EMS
II – EMS are produced by water nanostructures (naneons)
III – EMS are producing naneons
IV – Naneons and EMS carry specific DNA information
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EMS are produced by water nanostructures (naneons)
Evidence :
from filtration
Size : between 20 and 100 nM for bacterial sequences
Smaller that 20µM for viral sequences
from biophysical studies
indicating spectrometral changes in the dilutions
producing EMS
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Naneons and EMS carry
specific DNA information
Natural and digital transmission
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oligo
oligo
Taqpolymerase
Classical model of PCR
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PCR on water nanostructuresoligo
Taq
oligo
EMS
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DNA Water
Naneons
EMS EMS
DNA Water
NaneonsPCR
7 Hz
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-6
µmetal
Generator 7Hz
+ + +
-2 -3 -4 -5 -6
18hrs
water-6
Tube 2
water
EMS
Tube 1
DNA
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D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM
Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM
DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM
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DNA Water Naneons
EMS
DNA
Water Naneons
PCR
ComputerDigitized
Receiver
ComputerEMS Analog
Water-mediated photonic transmission of DNA
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Reproduction of DNA transduction
in other laboratories
File EMS of 194 bp DNA from HIV1 LTR
Sent to Benevento University,
Molecular Biology Laboratory
DNA reproduced and sequenced
100 % identical to original
File EMS of 499 bp DNA from Borrelia burgdorferi
Sent to Laboratory of Chronix Biomedicals
University of Gottingen
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Reproduction of DNA transduction in other laboratoriesFile EMS of 194 bp DNA from HIV1 LTRSent to Benevento University,Molecular Biology LaboratoryDNA reproduced and sequenced100 % identical to originalFile EMS of 499 bp DNA from Borrelia burgdorferiSent to Laboratory of Chronix BiomedicalsUniversity of Gottingen
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Water-mediated photonic transmission of DNA
Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)E.Schutz et al. Goettingen, 2011
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How pathogenic information can persist, and escape immune defence and treatment.
DNA
DNA
DNA
DNA
DNA
WATER(plasma)
ELF
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- The viral DNA reservoir- The Higher sensitivity of African people to HIV infection
- The viral DNA reservoir- The Higher sensitivity of African people to HIV infection
Two main problems for AIDS researchin 2013
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The dual origin of EMS in HIV/AIDS
EMS plasma RBC
HIV < 20 nM ++ +/-
Agent X < 100 nM - ++
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Evidence that the agent X is a bacterium
- Presence of a sequence close to 16 S ribosomal DNA of Rickettsiales- Sensitive to antibiotics ( in vitro and in vivo)- Growth in cell line- Electron microscopy- Associated with Red Blood Cells
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Human sequences associated with the agent X
237 bp (human chromosome 1)213 bp (human chromosome 7)
and more…
Human sequences associated with the agent X
237 bp (human chromosome 1)213 bp (human chromosome 7)
and more…
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Strong correlation with HIV infection
- Contains human DNA sequences emitting EMS only in pathogenic HIV infection
-Sequence 213 bp from Chromosome 7
EMS in HIV + patients
no EMS in HIV - patients
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Risk factor 2RBC
Risk factor 1RBC
HIV plasmaRBC
DNAPCR EMS+ +
++
-+
RISK FACTORS OF HIV INFECTION
(all HIV) (20 nM)
(213/237 bp) (100 nM) (HIV+) ( all HIV+, a few HIV-)
( all HIV+, many HIV-) (HIV- & HIV+) (100 nM)
(LTR 195 bp)
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« « A new intracellular bacteriumA new intracellular bacterium has has been found in human red blood been found in human red blood cells. A variant bearing extra DNA cells. A variant bearing extra DNA sequences is present in all HIV sequences is present in all HIV infected patients and also in some infected patients and also in some healthy HIV negative African healthy HIV negative African individuals”individuals”..
« « A new intracellular bacteriumA new intracellular bacterium has has been found in human red blood been found in human red blood cells. A variant bearing extra DNA cells. A variant bearing extra DNA sequences is present in all HIV sequences is present in all HIV infected patients and also in some infected patients and also in some healthy HIV negative African healthy HIV negative African individuals”individuals”..
CONCLUSION 1
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CONCLUSION 2
Origin of EMS :
not necessarily depending on DNA sequence
- depending on transmissible modification of DNA (free radicals of water ?)
- Association with pathogenicity
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AUTISM :
THE GUT, BLOOD and BRAIN MICROBIAL CONNECTION
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GUT BLOOD BRAIN
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20 -
30 -
10 -
40 -
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Correlation between EMS, antibiotic treatmentand clinical signs in an autistic child
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Environmental factorsgenetic
susceptibility
immunosuppression
Oxidative stress
Bacterial agents
Oxidative stress
« prion » effect
Less and less reversible
Reversible
Somatic mutations
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In press
Electromagnetic detection of HIV DNA in the blood of AIDS patients treated by antiretroviral therapy.
Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×, Mireille MBAMY , Joseph VARON⁰ #, and Henri CHENAL⁰
× World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France† Nanectis Biotechnologies, France
⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast# The University of Texas Health Science Center, Houston, USA.
Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.Key words: DNA, Electromagnetic signals, bacteria
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Centre Intégré de Recherche Bioclinique d’Abidjan
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World Foundation of AIDS Research and PreventionR. Olivier, Cl. Lavallee,H.Chenal, M. Mbamy,
Nanectis Biotechnologies SAJ.Aissa, Cl.Lavallee, R.Olivier
Goettingen University and Chronix BiomedicalsE. Schutz, H.Urnovitz
University of Washington, SeattleGerald H. Pollack
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“Absence of evidence is not
evidence of absence”
Carl Sagan