warm-up (11/14 & 11/15) look over the cell respiration test - make sure you understand why you...

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Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything • Pick up a test from the front When you are done, turn everything in to the front and retake the CBA as a group Write your new answers on your warm-up sheet We will go over the answers in 15 min 1

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Page 1: Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything Pick up a test from the front When you

Warm-up (11/14 & 11/15)• Look over the cell respiration test - make sure

you understand why you missed anything• Pick up a test from the front

• When you are done, turn everything in to the front and retake the CBA as a group• Write your new answers on your warm-up sheet• We will go over the answers in 15 min

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Page 2: Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything Pick up a test from the front When you

Objectives

• To understand how to predict the genotypes and phenotypes of offspring from different parents

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Homework

• Study for Quiz 1 – next time-Labeling pics of mitosis-Labeling cell cycle-Labeling a chromosome-Study the mitosis vs. meiosis chart

• Complete Punnett square worksheets• Begin working on Genetics brochure

-Due on 12/13 & 12/14

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Page 4: Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything Pick up a test from the front When you

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Mendelian Mendelian GeneticsGenetics

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Gregor Gregor MendelMendel

(1822-1884)(1822-1884)

Responsible Responsible for the Laws for the Laws governing governing

Inheritance Inheritance of Traitsof Traits

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Gregor Johann MendelGregor Johann MendelAustrian monkAustrian monkStudied the Studied the inheritanceinheritance of of traits in traits in pea plantspea plantsDeveloped the Developed the laws of inheritancelaws of inheritanceMendel's work Mendel's work was not recognized was not recognized until the turn of until the turn of thethe 20th century 20th century

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Gregor Johann MendelGregor Johann MendelBetween Between 1856 1856 and 1863,and 1863, Mendel Mendel cultivated and cultivated and tested some tested some 28,000 pea plants28,000 pea plantsHe found that He found that the plants' the plants' offspring retained offspring retained traits of the traits of the parents (heredity)parents (heredity)Called theCalled the “Father of “Father of Genetics"Genetics"

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Site of Site of Gregor Gregor Mendel’s Mendel’s experimentexperimental garden al garden in the in the Czech Czech RepublicRepublic

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Mendel stated Mendel stated that physical traits that physical traits are inherited as are inherited as “particles”“particles”

Mendel did not Mendel did not know that the know that the “particles” were “particles” were actually actually Chromosomes & Chromosomes & DNADNA

Particulate InheritanceParticulate Inheritance

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Genetic TerminologyGenetic TerminologyDefine these terms:Define these terms:TraitTrait - any characteristic - any characteristic that can be passed from that can be passed from parent to offspring parent to offspring HeredityHeredity - passing of traits - passing of traits from parent to offspring from parent to offspring GeneticsGenetics - study of heredity - study of heredity

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Types of Genetic Types of Genetic CrossesCrossesMonohybrid cross Monohybrid cross - - cross cross

involving a single traitinvolving a single traite.g. flower color e.g. flower color Dihybrid crossDihybrid cross - - cross cross involving two traits involving two traits e.g. flower color & plant e.g. flower color & plant heightheight

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Punnett SquarePunnett Square-Used to help -Used to help solve genetics solve genetics problemsproblems

-Can only show -Can only show the the possiblepossible results of a results of a genetic crossgenetic cross

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Page 14: Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything Pick up a test from the front When you

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Designer “Genes”Designer “Genes”

AllelesAlleles - - two forms of a two forms of a gene gene (dominant & recessive)(dominant & recessive) DominantDominant - - stronger of two stronger of two genes expressed in the hybrid; genes expressed in the hybrid; represented byrepresented by aa capital letter (R)capital letter (R) RecessiveRecessive - - gene that shows up gene that shows up less often in a cross; represented less often in a cross; represented by aby a lowercase letter (r)lowercase letter (r)

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More TerminologyMore Terminology

GenotypeGenotype - - gene gene combination for a traitcombination for a trait (e.g. (e.g. RR, Rr, rr) RR, Rr, rr)

PhenotypePhenotype - - the physical the physical feature resulting from a feature resulting from a genotypegenotype (e.g. red, white) (e.g. red, white)

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Genotype & Phenotype in Genotype & Phenotype in FlowersFlowers

Genotype of alleles:Genotype of alleles:RR = red flower= red flowerrr = yellow flower= yellow flower

All genes occur in pairs, so All genes occur in pairs, so 22 allelesalleles affect a characteristic affect a characteristic

Possible combinations are:Possible combinations are:

GenotypesGenotypes RRRR RRrr rrrr

PhenotypesPhenotypesRED RED RED RED YELLOWYELLOW

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GenotypesGenotypesHomozygousHomozygous genotype - gene genotype - gene

combination involving 2 combination involving 2 dominant or 2 recessive genes dominant or 2 recessive genes (e.g. RR or rr);(e.g. RR or rr); also called also called pure pure  HeterozygousHeterozygous genotype - gene genotype - gene combination of one dominant & combination of one dominant & one recessive allele    (one recessive allele    (e.g. Rr);e.g. Rr); also calledalso called hybridhybrid

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Genes and Environment Genes and Environment Determine CharacteristicsDetermine Characteristics

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Mendel’s Pea Mendel’s Pea Plant Plant

ExperimentsExperiments

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Why peas,Why peas, Pisum Pisum sativumsativum??Can be grown in a Can be grown in a

small areasmall area Produce Produce lots of lots of offspring offspring Produce Produce purepure plants when allowed plants when allowed to to self-pollinateself-pollinate several generations several generations Can be Can be artificially artificially cross-pollinatedcross-pollinated

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Reproduction in Flowering Reproduction in Flowering PlantsPlants

Pollen contains spermPollen contains spermProduced by the stamenProduced by the stamen

Ovary contains eggsOvary contains eggsFound inside the flowerFound inside the flower

Pollen carries sperm to Pollen carries sperm to the eggs for fertilizationthe eggs for fertilization

Self-fertilizationSelf-fertilization can can occur in the same occur in the same flowerflower

Cross-fertilizationCross-fertilization can can occur between occur between flowersflowers

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Mendel’s Mendel’s Experimental Experimental

MethodsMethodsMendel Mendel hand-hand-pollinatedpollinated flowers flowers using a using a paintbrushpaintbrush

He could He could snip the snip the stamensstamens to to prevent self-prevent self-pollinationpollination

He traced traits He traced traits through the through the several several generationsgenerations

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How Mendel BeganHow Mendel BeganMendel Mendel produced produced purepure strains by strains by allowing allowing the plants the plants to to self-self-pollinatepollinate for for several several generatiogenerationsns

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Eight Pea Plant TraitsEight Pea Plant Traits

Seed shapeSeed shape --- Round --- Round (R)(R) or Wrinkled or Wrinkled (r)(r)Seed ColorSeed Color ---- Yellow ---- Yellow (Y)(Y) or  Green or  Green (y)(y)Pod ShapePod Shape --- Smooth --- Smooth (S)(S) or wrinkled or wrinkled (s)(s)Pod ColorPod Color ---  Green ---  Green (G)(G) or Yellow or Yellow (g)(g)Seed Coat ColorSeed Coat Color ---Gray ---Gray (G)(G) or White or White (g)(g)Flower positionFlower position---Axial ---Axial (A)(A) or Terminal or Terminal (a)(a)Plant HeightPlant Height --- Tall --- Tall (T)(T) or Short or Short (t)(t)Flower color Flower color --- --- Purple Purple (P)(P) or white or white (p)(p)

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Page 26: Warm-up (11/14 & 11/15) Look over the cell respiration test - make sure you understand why you missed anything Pick up a test from the front When you

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Mendel’s Experimental Mendel’s Experimental ResultsResults

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Did the observed ratio match the theoretical ratio?Did the observed ratio match the theoretical ratio?

The theoretical or expected ratio of The theoretical or expected ratio of plants producing round or wrinkled plants producing round or wrinkled seeds is seeds is 3 round :1 wrinkled3 round :1 wrinkled

Mendel’s observed ratio was 2.96:1Mendel’s observed ratio was 2.96:1

The discrepancy is due to The discrepancy is due to statistical statistical errorerror

The The larger the samplelarger the sample the more the more nearly the results approximate to nearly the results approximate to the theoretical ratiothe theoretical ratio

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Generation “Gap”Generation “Gap”Parental PParental P11 Generation Generation = the parental = the parental

generation in a breeding experimentgeneration in a breeding experiment..

FF11 generation generation = the first-generation = the first-generation offspring in a breeding experiment. offspring in a breeding experiment. (1st filial generation)(1st filial generation)

From breeding individuals from the PFrom breeding individuals from the P11 generationgeneration

FF22 generation generation = the second-generation = the second-generation offspring in a breeding experiment. offspring in a breeding experiment. (2nd filial generation)(2nd filial generation) From breeding individuals from the From breeding individuals from the

FF11 generation generation

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Following the Following the GenerationsGenerations

Cross 2 Cross 2 Pure Pure

PlantsPlantsTT x ttTT x tt

Results Results in all in all

HybridsHybridsTtTt

Cross 2 Cross 2 HybridsHybrids

getget3 Tall & 1 3 Tall & 1

ShortShortTT, Tt, ttTT, Tt, tt

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Monohybrid Monohybrid CrossesCrosses

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Trait: Seed ShapeTrait: Seed Shape

Alleles: Alleles: RR – Round – Round rr – Wrinkled – Wrinkled

Cross: Cross: RoundRound seedsseeds xx Round Round seedsseeds

RrRr xx Rr Rr

PP11 Monohybrid Cross Monohybrid Cross

R

r

rR

RR

rrRr

Rr

Genotype:Genotype: RR Rr rrRR Rr rr

PhenotypePhenotype: Round and Round and wrinkledwrinkled

GenotypicGenotypicRatio:Ratio: 1:2:1 1:2:1 PhenotypicPhenotypicRatio:Ratio: 3:1 3:1

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PP11 Monohybrid Cross Monohybrid Cross ReviewReviewHomozygous dominant x Homozygous dominant x

Homozygous recessiveHomozygous recessiveOffspringOffspring allall HeterozygousHeterozygous (hybrids)(hybrids)Offspring calledOffspring called FF11 generation generation

Genotypic & Phenotypic ratio Genotypic & Phenotypic ratio isis ALL ALIKEALL ALIKE

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Trait: Seed ShapeTrait: Seed Shape

Alleles: Alleles: RR – Round – Round rr – Wrinkled – Wrinkled

Cross: Cross: RoundRound seeds seeds xx Round Round seedsseeds

RrRr xx Rr Rr

FF11 Monohybrid Cross Monohybrid Cross

R

r

rR

RR

rrRr

Rr

Genotype:Genotype: RR, Rr, RR, Rr, rrrr

PhenotypePhenotype: Round Round && wrinkled wrinkled

G.Ratio:G.Ratio: 1:2:11:2:1

P.Ratio:P.Ratio: 3:1 3:1

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FF11 Monohybrid Cross Monohybrid Cross ReviewReviewHeterozygous x heterozygousHeterozygous x heterozygousOffspring:Offspring:

25% Homozygous dominant25% Homozygous dominant RRRR50% Heterozygous50% Heterozygous RrRr25% Homozygous Recessive25% Homozygous Recessive rrrrOffspring calledOffspring called FF22 generation generationGenotypic ratio isGenotypic ratio is 1:2:11:2:1Phenotypic RatioPhenotypic Ratio is 3:1 is 3:1

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What Do the Peas Look What Do the Peas Look Like?Like?

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……And Now the Test And Now the Test CrossCross

Mendel then crossed a Mendel then crossed a purepure & a & a hybridhybrid from his from his FF2 2 generationgeneration

This is known as an This is known as an FF22 or test cross or test cross

There are There are twotwo possible testcrosses: possible testcrosses:Homozygous dominant x HybridHomozygous dominant x HybridHomozygous recessive x HybridHomozygous recessive x Hybrid

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Trait: Seed ShapeTrait: Seed Shape

Alleles: Alleles: RR – Round – Round rr – Wrinkled – Wrinkled

Cross: Cross: RoundRound seedsseeds xx Round Round seedsseeds

RRRR xx Rr Rr

FF22 Monohybrid Cross (1 Monohybrid Cross (1stst))

R

R

rR

RR

RrRR

Rr

Genotype:Genotype: RR, RR, RrRr

PhenotypePhenotype: RoundRound

GenotypicGenotypicRatio:Ratio: 1:11:1

PhenotypicPhenotypicRatio:Ratio: All alike All alike

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Trait: Seed ShapeTrait: Seed Shape

Alleles: Alleles: RR – Round – Round rr – Wrinkled – Wrinkled

Cross: Cross: WrinkledWrinkled seedsseeds xx Round Round seedsseeds

rrrr xx Rr Rr

FF22 Monohybrid Cross (2nd) Monohybrid Cross (2nd)

r

r

rR

Rr

rrRr

rr

Genotype:Genotype: Rr, rrRr, rr

PhenotypePhenotype: Round & Round & WrinkledWrinkled

G. Ratio:G. Ratio: 1:11:1

P.Ratio:P.Ratio: 1:1 1:1

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FF22 Monohybrid Cross Monohybrid Cross ReviewReviewHomozygous x Homozygous x

heterozygous(hybrid)heterozygous(hybrid)Offspring:Offspring:50% Homozygous 50% Homozygous RR or rrRR or rr50% Heterozygous50% Heterozygous RrRrPhenotypic RatioPhenotypic Ratio is 1:1 is 1:1Called Called Test CrossTest Cross because the because the offspring have offspring have SAMESAME genotype genotype as parentsas parents

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Warm-up (11/16 & 11/26)-Have your Genetics notes out

-If you didn’t pick a genetic disorder before the break, see me to pick one

E = Free ear lobes e = attached ear lobesCross a heterozygous free-eared male with a woman

with attached ear lobes1.Write the genotypes of the parents2.Write what genes each parent could have in

each gamete3.Draw the Punnett square4.List the genotypic and phenotypic percentages5.List the genotypic and phenotypic ratios

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Mendel’s LawsMendel’s Laws

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Results of Monohybrid Results of Monohybrid CrossesCrosses

InheritableInheritable factors or genesfactors or genes are are responsible for all heritable responsible for all heritable characteristics characteristics

PhenotypePhenotype is based on is based on GenotypeGenotype Each traitEach trait is based onis based on two genestwo genes, ,

one from the mother and the one from the mother and the other from the fatherother from the father

True-breeding individuals are True-breeding individuals are homozygous ( both alleles)homozygous ( both alleles) are are the samethe same

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1. Law of Dominance1. Law of Dominance

In a cross of parents that are In a cross of parents that are pure for contrasting traitspure for contrasting traits, , only one form of the trait will only one form of the trait will appear in the next generation.appear in the next generation.

All the offspring will be All the offspring will be heterozygous and express heterozygous and express only the only the dominant trait.dominant trait.

RR x rr RR x rr yieldsyields all Rr (round all Rr (round seeds)seeds)

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Law of DominanceLaw of Dominance

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2. Law of Segregation2. Law of Segregation

During the During the formation of formation of gametesgametes (eggs or sperm), the (eggs or sperm), the two allelestwo alleles responsible for a responsible for a trait trait separateseparate from each other. from each other.

Alleles for a trait are then Alleles for a trait are then "recombined" at fertilization"recombined" at fertilization, , producing the genotype for producing the genotype for the traits of the offspringthe traits of the offspring.

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Applying the Law of Applying the Law of SegregationSegregation

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3. Law of Independent 3. Law of Independent AssortmentAssortment

Alleles for Alleles for differentdifferent traits are traits are distributed to sex cells (& distributed to sex cells (& offspring) independently of offspring) independently of one another.one another.

This law can be illustrated This law can be illustrated using using dihybrid crossesdihybrid crosses..

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Independent Assortment

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Dihybrid CrossDihybrid CrossA breeding experiment that tracks A breeding experiment that tracks

the the inheritance of two traitsinheritance of two traits..

Mendel’s Mendel’s “Law of Independent “Law of Independent Assortment”Assortment”

a. Each pair of alleles segregates a. Each pair of alleles segregates independentlyindependently during gamete during gamete formationformation

b. Formula: 2b. Formula: 2nn (n = # of heterozygotes) (n = # of heterozygotes)

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Question:Question:How many gametes will be How many gametes will be

produced for the following allele produced for the following allele arrangements?arrangements?

Remember:Remember: 22nn (n = # of heterozygotes) (n = # of heterozygotes)

1.1. RrYyRrYy

2.2. AaBbCCDdAaBbCCDd

3.3. MmNnOoPPQQRrssTtQqMmNnOoPPQQRrssTtQq

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Answer:Answer:1. RrYy: 21. RrYy: 2nn = 2 = 222 = 4 gametes = 4 gametes

RY Ry rY ryRY Ry rY ry

2. AaBbCCDd: 22. AaBbCCDd: 2nn = 2 = 233 = 8 gametes = 8 gametes

ABCD ABCd AbCD AbCdABCD ABCd AbCD AbCd

aBCD aBCd abCD abCDaBCD aBCd abCD abCD

3. MmNnOoPPQQRrssTtQq: 23. MmNnOoPPQQRrssTtQq: 2nn = 2 = 266 = = 64 gametes64 gametes

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Dihybrid CrossDihybrid CrossTraits: Seed shape & Seed colorTraits: Seed shape & Seed colorAlleles:Alleles: R round

r wrinkled Y yellow y green

RrYy x RrYy

RY Ry rY ryRY Ry rY ry RY Ry rY ryRY Ry rY ry

All possible gamete combinationsAll possible gamete combinations

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Dihybrid CrossDihybrid Cross

RYRY RyRy rYrY ryry

RYRY

RyRy

rYrY

ryry

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Dihybrid CrossDihybrid Cross

RRYY

RRYy

RrYY

RrYy

RRYy

RRyy

RrYy

Rryy

RrYY

RrYy

rrYY

rrYy

RrYy

Rryy

rrYy

rryy

Round/Yellow: 9

Round/green: 3

wrinkled/Yellow: 3

wrinkled/green: 1

9:3:3:1 phenotypic ratio

RYRY RyRy rYrY ryry

RYRY

RyRy

rYrY

ryry

RrYy x RrYy

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Dihybrid CrossDihybrid Cross

Round/Yellow: 9Round/green: 3wrinkled/Yellow: 3wrinkled/green: 1

9:3:3:1

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Test CrossTest CrossA mating between an individual of A mating between an individual of unknown unknown

genotype genotype and a and a homozygous recessivehomozygous recessive individual.individual.

Example:Example: bbC__ bbC__ x x bbccbbcc

BB = brown eyesBB = brown eyesBb = brown eyesBb = brown eyesbb = blue eyesbb = blue eyes

CC = curly hairCC = curly hairCc = curly hairCc = curly haircc = straight haircc = straight hair

bCbC b___b___

bcbc

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Test CrossTest Cross

Possible results:Possible results:

bCbC b___b___

bcbc bbCc bbCc

C bCbC b___b___

bcbc bbCc bbccor

c

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Summary of Mendel’s Summary of Mendel’s lawslaws

LAWLAW PARENT PARENT CROSSCROSS OFFSPRINGOFFSPRING

DOMINANCEDOMINANCE TT x ttTT x tt tall x shorttall x short

100% Tt 100% Tt talltall

SEGREGATIONSEGREGATION Tt x TtTt x Tt tall x talltall x tall

75% tall 75% tall 25% short25% short

INDEPENDENT INDEPENDENT ASSORTMENTASSORTMENT

RrGg x RrGgRrGg x RrGg round & round & green x green x round & round & greengreen

9/16 round seeds & green 9/16 round seeds & green pods pods

3/16 round seeds & yellow 3/16 round seeds & yellow pods pods

3/16 wrinkled seeds & 3/16 wrinkled seeds & green pods green pods

1/16 wrinkled seeds & 1/16 wrinkled seeds & yellow podsyellow pods

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Quiz 1-Labeling pics of mitosis-Labeling cell cycle-Labeling a chromosome-Study the mitosis vs. meiosis chart

Work on Dihybrid WS after you are done

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