Vol. 257, No. 13 The Journal of July 10, 1982

Biological Chemistry Copyright 0 1982 by the American Society of Biological Chemists, Inc., 428 East Preston St., Baltimore, Md. 21202 U.S.A.

CONTENTS*

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7306

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7318

COMMUNICATIONS 7348 Inhibition of milk xanthine oxidase by fluorodinitro-

Takeshi Nishino, Keizo Tsushima, Russ Hille, and Vin-

7354 The isolated y subunit of Escherichia coli FI ATPase

Coordinate accumulation of the mRNAs for the a, /3, and y chains of rat fibrinogen following defibrination.

DNA primase activity from human lymphocytes. Syn- thesis of oligoribonucleotides that prime DNA synthe-

benzene.

cent Massey

binds the subunit. Stanley D. Dunn

Gerald R. Crabtree and Jeffrey A. Kant

SIS. Ben Y. Tseng and Clarence N . Ahlem

Analysis of regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in a somatic cell mutant auxo- trophic for mevalonate.

lokoff, and Peter A . Edurards Michael Sinensky, Robert Torget, Robin Schnitzer-Po-

Maitotoxin, a CaZ+ channel activator candidate. Masami Takahashi, Yasushi Ohizumi, and Takeshi Yas-

umoto Cytochalasin B. A natural photoaffinity ligand for la- beling the human erythrocyte glucose transporter.

Dopamine-derived tetrahydroisoquinolines. Novel in- hibitors of dihydropteridine reductase.

Abram Brubaker, and Creed W. Abell Rong-sen Shen. Robert V. Smith, Patrick J . Daris,

Phosphotyrosyl-protein phosphatase of TCRC-2 cells. Ghanshyam Sularup, K. Vincent Specg, Jr., Stanley

Cohen, and Dartid L. Garbers Enhancement of 5-lipoxygenase activity in mastocy- toma P-815 cells by n-butyrate treatment.

Yasuko Koshihara, Mariko Mirumura, and Sei-itsu Mu- rota

tic smooth muscle. Purification of a myosin phosphatase from bovine aor-

Diane K. Werth, Joe R. Haeberle, and Dauid R. Hatha- way Differential anticoagulant activity of heparin frag- ments prepared using microbial heparinase.

Robert J. Linhardt, Arthur Grant, Charles L. Cooney, and Robert Langer

inositol cycle. Appearance of phosphatidic acid is as- Platelet-activating factor stimulates the phosphatidyl-

sociated with the release of serotonin in horse plate- lets.

Solubilization and localization of cholesteryl oleate in egg phosphatidylcholine vesicles. A carbon 13 NMR study.

Michael F. Shanahan

Eduardo G. Laprtina

James A. Hamilton and Vonald M. Small

7322 Nucleotide sequences of switch regions of immuno- globulin C, and C., genes and their comparison.

suku Honjo Toshio Nikaido, Yuriko Yamaulaki-Kataoka, and T a -

7330 Genetic variation in glycosylation of the fourth com- ponent of murine complement. Association with he- molytic activity.

fler Daoid R. Karp, John P. Atkinson, and Donald C. Shref-

7336 Histone acetylation increases the solubility of chro- matin and occurs sequentially over most of the chro- matin. A novel model for the biological role of histone acetylation.

Michael Perry and Roger Chalkley

7360 Kinetics of the heparin-enhanced antithrombin III/ thrombin reaction. Evidence for a template model for the mechanism of action of heparin.

Michael J . Griffith 7366 Direct fluorescence measurements of Mg2+ binding to

Florent Guillain. Marcel P. Gingold, and Phzlppe Cham-

7372 Study of the structure of troponin-I by measuring the

sarcoplasmic reticulum ATPase.

peil

relative reactivities of lysines yith acetic anhydride. Sarah E. Nitchcock.De Gregw-1

7381 Arrangement of attachment-promoting, self-associa- tion, and heparin-binding sites in horse serum fibro- - nectin.

and Dac>id C. Turner Ruth Ehrismann, Donncr I?'. Koth, Hans M. b,'ppen herger,

7388 A thermostable tRNA (guanosine-2'-)-methyltransfer-

ribose methylation on the conformational stability of ase from Thermus thermophilus HB27 and the effect of

tRNA. Izumi Kumagai, Kimitsuna Watanabe, and Tairo Osh-

ima 7396 Enzymatic synthesis, characterization, and metabo-

lism of the coenzyme A ester of o-succinylbenzoic acid,

thesis. an intermediate in menaquinone (vitamin Kz) biosyn-

Lutz Heidk, Sibylle Arendt, and Eckhard Leistner 7401 Structure of the w-aminocarboxylic acid-binding sites

of human plasminogen. Arginine 70 and aspartic acid 56 are essential for binding of ligand by Kringle 4.

Maria Trexler, Zsofia Vcili, and Laiszlb Patthy 7407 Study of a proline-rich polypeptide bound to the pros-

Walter Heyns, Denise Bossyns, Ben Peeters, and Wilfried

7414 Vanadate-induced movements of Ca'+ and K+ in hu-

tatic binding protein of rat ventral prostate.

Rombauts

man red blood cells. Ludouit Varecka and Ernesto Carafoli

7422 Isolation and characterization of rat liver microsomal UDP-glucuronosyltransferase activity toward cheno- deoxycholic acid and testosterone as a single form of enzyme.

Heidrun Matern, Siegfried Matern, and Wolfgang Gerok 7430 Effects of angiotensin I1 on phosphatidylinositol and

polyphosphoinositide turnover in rat kidney. Mecha- nism of prostaglandin release.

Julio E. Benahe, Leslie A . Spry, and Aubrey R. Morrison 7435 Evidence for the organization of the transmembrane

embedded and surface domains of the a-subunit. segments of (Na,K)-ATPase based on labeling lipid-

Peter L. Jvrgensen, Stellen J . D. Karlish, and Carlos Gitler

7443 Identification of an insulin-sensitive calcium-stimu- lated phosphoprotein in rat adipocyte plasma mem- branes.

Kuwk-Ming Chan and Jay M. McDonald

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in THE JOUKNAL, 257, 1 (1982), and reprints may be obtained from the editorial office.

2

3

7449 Kinetic investigation of rat liver microsomal electron transport from NADH to cytochrome P-450.

Gary J . Fisher and James L. Guylor 7456 Binding of calcium ions to the isolated asialo-glycopro-

tein receptor. Implications for receptor function in suspended hepatocytes.

Rune Blomhoff, Helge Tolleshaug, and Trond Berg 7460 Purification and characterization of an a-ketoisocap-

roate oxygenase of rat liver. Patrick J . Sabourin and Loran L. Bieber

7468 The mechanism of a-ketoisocaproate oxygenase. For- mation of P-hydroxyisovalerate from a-ketoisocapro- ate.

Patrick J . Sabourin and Loran L. Bieber 7472 Defective in vitro lipolysis of type IV hyperlipidemic

human plasma by purified milk lipoprotein lipase. Studies by single vertical spin centrifugation.

Byung Hong Chung, John T. Cone, and Jere P. Segrest 7481 1,25-dihydroxyvitamin D receptors in an established

bone cell line. Correlation with biochemical responses.

man, und Richard A. Luben Marian R. Walters, David M. Rosen, Anthony W. Nor-

7485 Regulation of adenylate cyclase of human platelet membranes by forskolin.

,Jucyue.s Hanoune Paul A. Insel, Dominique Stengel, Nicolas Ferry, and

7491 Evidence for a monoglucuronide of 1,25-dihydroxyvi- tamin DS in rat bile.

Rajiu Kumar Robert D. Lituiller, Vernon R. Mattox, Ian Jardine, and

7495 Entry of the toxic proteins abrin, modeccin, ricin, and diphtheria toxin into cells. I. Requirement for calcium.

7504 Entry of the toxic proteins abrin, modeccin, ricin, and diphtheria toxin into cells. 11. Effect of pH, metabolic inhibitors, and ionophores and evidence for toxin pen- etration from endocytotic vesicles.

Kirsten Sandrig and Sjur Olsnes

Kirsten Sandvig and SJur Olsnes

7514 Energetics of y-aminobutyrate transport in rat brain

rlnna Pastuszko, David F. Wilson, and Marla Erecinska 7520 Lectin from embryos and oocytes of Xenopus [aeuis.

synaptosomes.

Purification and properties. Marie M. Roberson and Samuel H. Barondes

7525 Effect of cross-linking between 82pI and 82p2 lysyl residues on the kinetics of polymerization of deoxy- hemoglobin S.

chi Klyomi Kikuguwa. Toshio Asakura, and Kazuhiko Ada-

7531 Arylamine N-methyltransferase. Methylation of the in- dole ring.

Ellen Sue Lyon and William B. Jakoby 7536 Structure of mouse submaxillary gland renin. Identi-

fication of two disulfide-linked polypeptide chains and the complete amino acid sequence of the light chain.

Kunio S. Misono and Tadashi Znagami 7541 Regulation of the phosphorylation and activity of 6-

phosphofructo 1-kinase in isolated hepatocytes by a- glycerolphosphate and fructose 2,6-bisphosphate.

Thomas H. Claus, John R. Schlumpf. M. Raafut E l - Maghrabi, and Simon J . Pilhis

7549 Effect of androgens on turnover of ornithine decarbox- ylase in mouse kidney. Studies using labeling of the enzyme by reaction with [14C]a-difluoromethylorni- thine.

cJames E. Serly. Hannu POso, and Anthony E . Pegg 7554 Reductive methylation of the two 4,4‘-diisothiocyano-

dihydrostilbene-2,2’-disulfonate-binding lysine resi- dues of band 3, the human erythrocyte anion trans- port protein.

Michael L. Jennings 7560 The exchange of Fe:” between acetohydroxamic acid

and transferrin. Spectrophotometric evidence for a mixed ligand complex.

Richard E. Cowart, Nakao Kojima, and George W. Bates 7566 Thyrotropin-releasing hormone rapidly and tran-

siently stimulates cytosolic calcium-dependent protein phosphorylation in GH, pituitary cells.

Debra S . Drust and Thomas F. J . Martin

7574 Purification and macromolecular properties of a sialic acid-specific lectin from the slug L i m a flauus.

Fish Ronald L. Miller, James F. Colluwn, Jr., and Wayne W.

7581 Isolation and purification of collagen al(1) receptor from human platelet membrane.

Thomas M. Chiang and Andreuj H. Kang 7587 Metabolic compartments in Dictyostelium discoideum.

Barbara E. Wright, David A. Thomas, and Debra J . Ingalls

7595 Hepatic pyruvate kinase. Quantitative measurements of phosphorylation in vitro and in the isolated rat hepatocyte.

James B. Blair, Michele A. Cimbala. and Milinda E . James

7603 Regulation of rat liver fructose 2,6-bisphosphatase. M. Raafat El-Maghmbi, Thomas H. Claus, Jo Pzlkis.

7608 Heterogeneity of rabbit intestine brush border plasma Eric Fox, and Simon <J. Pilkis

membrane cholesterol. Bernabe BloJ and Donald B. Ziluersmit

7615 Subunit interaction in catalysis. Some experimental and theoretical approaches with glyceraldehyde-3- phosphate dehydrogenase.

Jeffrey W. Cardon and Paul D. Boyer 7623 Biosynthesis, intracellular transport, and release of

the Golgi enzyme galactosyltransferase (lactose syn- thetase A protein) in HeLa cells.

Ger J . A. M. Strous and Erlc G. Bergrr 7629 Rapid changes in the concentration of phosphoenol-

pyruvate carboxykinase mRNA in rat liver and kidney. Effects of insulin and cyclic AMP.

son, John E . Monahan, Heeja Yoo- Warren, and Richard Michele A . Clmbala, Wouter H. Lamers, Kathleen Nel-

W. Hanson 7637 Protein glycosylation in Trypanosoma cruzi. 1. Char-

oligosaccharides synthesized “in vivo.” acterization of dolichol-bound monosaccharides and

Armando J . Parodi and Luis A . Quesada-Allue

764 1 Protein glycosylation in Trypanosoma cruzi. 11. Partial characterization of protein-bound oligosaccharides la- beled “in vivo.”

Armando J . Parodi and Juan J . Carrulo 7646 Reactivity and metal-dependent stereospecificity of

the phosphorothioate analogs of ATP in the arginine kinase reaction. Structure of the metal-nucleoside tri- phosphate substrate.

Mildred Cohn, Nancy Shih, and Jerelyn Nick 7650 Reactivity and metal-dependent stereospecificity of

the phosphorothioate analogs of ADP and ATP and reactivity of Cr(II1)ATP in the 3-phosphoglycerate ki- nase reaction. Structure of the metal nucleotide sub- strates.

Eileen K. Juffe, Jerelyn Nick, and Mildred Cohn

7657 Phosphoenzyme formation from ATP in the ATPase of sarcoplasmic reticulum. Effect of KC1 or ATP and slow dissociation of ATP from precursor enzyme-ATP com- plex.

Munekazu Shigekaula and Tohru Kanazaua 7666 Role of exogenous cholesterol in regulation of adrenal

Atie H. Verschoor-KlootuJyk, Louis Verschoor, Salman

7672 Iron binding by horse spleen apoferritin. A vanadyl

steroidogenesis in the rat.

Azhar, and Gerald M. Reauen

(IV) EPR spin probe study. N. Dennis Chasteen and Elizabeth C. Theil

7678 A structural role for the Ca2+-M&+ sites on troponin C in the regulation of muscle contraction. Preparation and properties of troponin C depleted myofibrils.

7684 A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxy- adenosine triphosphate.

Henry G. Zot and James D. Potter

Paul J. Romaniuk and Fritz Eckstein

7689 Template-primer-dependent turnover of (S,)-dATPaS by T4 DNA polymerase. The stereochemistry of the associated 3‘ - 5’-exonuclease.

Amur Gupta, Charles DeBrosse, and Stephen J . Benkouic

4

7693

7698

7704

7712

7720

7730

7737

7746

7756

7762

7768

7770

7778

7786

7794

7803

7808

Effect of retinol status on retinol-binding protein bio- synthesis rate and translatable messenger RNA level in rat liver.

Dianne Robert Soprano. John Edgar Smith. and De Witt S . Goodman Isolation and characterization of a new fucoganglio-

hepatoma induced by N-2-acetylaminofluorene. side accumulated in precancerous rat liver and in rat

Permeability of canine cardiac sarcoplasmic reticulum vesicles to K', Na+, H+, and CI-.

Eukaryotic ribosomal subunit anti-association activity of calf liver is contained in a single polypeptide chain protein of M , = 25,500 (eukaryotic initiation factor 6).

Mactra DaLid M. Valenzuela, Asok Chaudhuri, and Umadas

Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes.

Robert Brackenbury, Reinhard Mailhammer, Urs Hutis- Stanley Hoffman, Barbara C. Sorkin, Perrin C. White,

hauser, Bruce A . Cunningham, and Gerald M. Edelman

Eric H. Holmes and Sen-itiroh Hakomori

Gerhard Meissner and Dana McKmlev

Chromatin structure of the chicken /3-globin gene re- gion. Sensitivity to DNase I, micrococcal nuclease, and DNase 11.

William I. Wood and Gary Feisenfeld Thymus myosin. Isolation and characterization of myosin from calf thymus and thymic lymphocytes, and studies on the effect of phosphorylation of its M , = 20,000 light chain.

hahn, Ute Groschel-Steulart. and John Kendrick-Jones Jonathan M. Scholey, Robin C. Smith, Detlec Drenck-

Characterization of Nicotiana tabacum chloroplast and cytoplasmic ribosomal proteins.

The molecular characterization of three transcrip- tional mutations in the yeast iso-2-cytochrome c gene.

Donna L. Montgomer-v, Jeremy M. Boss, Stephen J . McAndreu), Linda Marr, Deborah A. Walthall, and Rich- ard S. Zitomer Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley.

Guy Bellemare, Sue G. Bartlett, and Nam-Hai Chua

peroxidase. Crystallization and preliminary x-ray data for chloro-

Byron Rubin, Jody VanMiddlesworth, Kenneth Thomas, and Lowell Hager Structure of hepatitis B surface antigen. Characteri- zation of the lipid components and their association

Malcolm S. Cupel and Don P. Bourque

with the viral proteins. Francisco Gavilanes, Jose M. Gonzalez-Ros, and Darrell

L. Peterson

ygenase. Amino acid composition and sites of action of Purification and properties of bovine spleen heme ox-

inhibitors of heme oxidation. Takeo Yoshinaga, Shigeru Sassa, and Attallah Kappas

The occurrence of molecular interactions among NADPH-cytochrome c reductase, heme oxygenase, and biliverdin reductase in heme degradation.

Takeo Yoshinaga, Shigeru Sassa, and Attallah Kappas A comparative study of heme degradation by NADPH- cytochrome c reductase alone and by the complete heme oxygenase system. Distinctive aspects of heme degradation by NADPH-cytochrome c reductase.

Takeo Yoshinaga, Shigeru Sassa, and Attallah Kappas The oxidative degradation of heme c by the micro- somal heme oxygenase system.

Takeo Yoshinaga, Shigeru Sassa, and Attallah Kappas Poly(ADP-ribosylation) in uitro. Reaction parameters and enzyme mechanism.

Ari M. Ferro and Baldomero M. OliL'era

and back copies to: THE JOURNAL OF BIOLOGICAL CHEMISTRY, 428 Address all correspondence and orders relative to subscriptions

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7814

7820

7826

7834

7839

7847

7852

7857

7864

7872

7879

7887

7898

7903

7910

7910

Normalization of intracellular lysosomal hydrolases in I-cell disease fibroblasts with sucrose loading.

Inui, Tohru Yutaka, and Hyakuji Yabuuchi Tomochikn Kato, Shintaro Okada, Toshio Ohshima, KoJi

Action of mung bean nuclease on supercoiled PM2 DNA.

David Kouralski and Janet P. Sanford Structure of the Syrian hamster ribosomal DNA repeat and identification of homologous and nonhomologous regions shared by human and hamster ribosomal DNAs.

Steven J . Triezenberg, Caroline Rushford, Ronald P. Hart, Kathleen L. Berkner, and William R. Folk Pretranslational regulation of an,-globulin in rat liver by growth hormone.

Arun K. Roy, Bandana Chatterjee, William F. Demyan, T . Surend Nath, and Nalini M. Motwant

of H-9-encoded proteins on target cell plasma mem- Physical separation and biochemical characterization

branes and endoplasmic reticulum.

Direct activation of calcium-activated, phospholipid- dependent protein kinase by tumor-promoting phorbol

Penny J. Gilmer

esters.

mihiko Sano, Ushio Kikkaua, and Yasutomi Nishizuka Monique Castagna, Yoshimi Takai, Kozo Kaibuchi, Ki-

Human cartilage collagens. Comparison of cartilage collagens with human type V collagen.

Morris, and Dacid W. Hollister Robert E. Burgeson, Patricia A . Hebda, Nicholas P.

Transcription and precursor processing of normal and mutant human tRNAi"' genes in a homologous cell- free system.

Rosenberg Michael Zasloff, Tomas Santos, Paul Romeo, and Martin

Maturation of the tail spike endorhamnosidase of Sal- monella phage P22.

King David P. Goldenberg, Peter B. Berget, and Jonathan

Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantification.

Robert K. Ockner, Joan A. Manning, and John P. Kane Purification and polypeptide characterization of com- plex I11 from yeast mitochondria.

Structure of amplified normal and variant dihydrofo- late reductase genes in mouse sarcoma Sl80 cells.

Eman, and Robert T. Schimke Gray F. Crouse, Christian C. Simonsen, Robert N. Mc-

The isolation of homogeneous leader peptidase from a

zyme. strain of Escherichia coli which overproduces the en-

Paul B. Wolfe, Pamela Silver, and William Wickner In vitro translation and processing of a precursor form of favin, a lectin from Vicia faba.

ningham John J . Hemperly, Keith E. Mostov, and Bruce A. Cun-

Anita Sidhu and Diana S. Beattie

ADDITIONS AND CORRECTIONS

Nucleotide sequence of Escherichia coli purF and de- duced amino acid sequence of glutamine phosphori- bosylpyrophosphate amidotransferase. Vol. 257 (1982) 3525-3531.

J. Yun Tso, Howard Zalkin, Magda van Cleemput, Charles Yanofsky, and John M. Smith

in 3T3-Ll adipocytes. Altered rate of receptor inacti- Insulin-induced down-regulation of insulin receptors

vation. Vol. 257 (1982) 4285-4291.

Lane Gabriele V. Ronnett, Victoria P. Knutson, and M. Daniel

Chemists, Inc. (Annual volume: United States, $285; other countries, Published semimonthly by the American Society of Biological

$325. Single copy: United States, $12.50 other countries, $14.50.) Subscriptions are entered on a calendar year basis only. Special rates are available to members, graduate students, and postdoctoral fellows for personal use only. Qualifying forms are available from the Bethesda Office. Second class postage paid at Baltimore, MD 21202, U.S.A., and at additional mailing offices.

Vol. 257, No. 13 The Journal of Biological Chemistry July 10, 1982

CONTENTS Arranged by Subject Categories

CARBOHYDRATES, LIP1I)S. ANI) OTHER NATURAL PRODUCTS

7430 Effects of angiotensin I1 on phosphatidylinositol and polyphosphoinositide turnover in rat kidney. Mecha-

07302

-7310

7396

7472

7491

7637

764 I

7666

i692

7698

”?

I i I O

7872

-7284

-7287

-7914

7407

Enhancement of 5-lipoxygenase activity in mastocy- toma P-815 cells by n-butyrate treatment.

Yasuko Koshihara, Mariko Mizumura, and Sei-itsu Mu- rota Differential anticoagulant activity of heparin frag- ments prepared using microbial heparinase.

and Robert Langer Robert J. Linhardt, Arthur Grant, Charles L. Cooney,

Enzymatic synthesis, characterization, and metabo- lism of the coenzyme A ester of o-succinylbenzoic acid, an intermediate in menaquinone (vitamin Kz) biosynthesis.

Defective in u i tm lipolysis of type IV hyperlipidemic human plasma by purified milk lipoprotein lipase. Studies by single vertical spin centrifugation.

Byung Hong Chung, John T. Cone, and Jere P. Segrest Evidence for a monoglucuronide of 1,25-dihydroxy- vitamin D:, in rat bile.

Kohert D. Lituiller, Vernon H. Matto.u, Ian Jccrdine, nnd Rajil* Kconcrr Protein glycosylation in Trypanosoma cruri. I. Char- acterization of dolichol-bound monosaccharides and oligosaccharides synthesized “in vivo.”

Protein glycosylation in Trypanosoma cruzi. 11. Par- tial characterization of protein-bound oligosaccha- rides labeled “in uiuo.”

Role of exogenous cholesterol in regulation of adrenal steroidogenesis in the rat.

Atie H. ~7erschoor-Klvot~~~vk, Lours C‘erschoor, Srclman Arhur, and Gerald M. Kenclen Effect of retinol status on retinol-binding protein bio- synthesis rate and translatable messenger RNA level in rat liver.

S. C;oodmun Dinnne Rohert Soprano, John Rdgar Smith, and DeU’itt

Isolation and characterization of a new fucoganglio-

hepatoma induced by N-2-acetylaminofluorene. side accumulated in precancerous rat liver and in rat

Structure of hepatitis B surface antigen. Characteri-

with the viral proteins. zation of the lipid components and their association

Frnncww Gacilanes. ,Jo,\c M . Gonznlez-Kos, nnrl Dar- rell L. Peterson Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantifica- tion.

Rohert K. Ockner, Jonn A . Munning, and cJohn P. Knne

Lutz Heide, Sibylle Arendt, and Eckhard Leistner

Amtundo J . Pnrodi a n d Luis A . Quesudn-Ailue

Armundo J. Parodi rmd Juun J . Cnzrulo

Eric H. Holmes crnd Sen-itiroh Hakonlori

CELL BIOLOGY ANI) METABOLISM Analysis of regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in a somatic cell mutant aux- otrophic for mevalonate.

Mlchnrl Sinmsiw, Robert Torget. Kohin Schnitzer-Po- lohoff. rend Peter A . Edrc1arcl.s

Maitotoxin, a Ca2+ channel activator candidate.

)’cl.sfmloto Masfln2l Taknhnshl. Ynsushi Ohizumi. nnri Tnkeshl

Platelet-activating factor stimulates the phosphati- dylinositol cycle. Appearance of phosphatidic acid is associated with the release of serotonin in horse platelets.

tatic binding protein of rat ventral prostate. Study of a proline-rich polypeptide bound to the pros-

Walter Heyns, Denise Bossyns, Ben Peeters, and Wil- fried Rombauts

Edunrdo G . Lrrpetinrc

nism- of prostaglandin release. Julio E. Benahe, Leslie A. Spry, and Aubrey R. Morrison

7443 Identification of an insulin-sensitive cakium-stimu- lated phosphoprotein in rat adipocyte plasma mem- . . branei.

K u d - M i n g Chan and Jay M. McDonald 7456 Binding of calcium ions to the isolated asialo-glyco-

protein receptor. Implications for receptor function in suspended hepatocytes.

Rune Blomhoff, Helge Tolleshaug, and Trond Berg 7481 1,25-dihydroxyvitamin D receptors in an established

bone cell line. Correlation with biochemical re- sponses.

Marian R. Walters, Dacid M. Kosm, Anthony W. Nor- man, and Richard A. Luben

7495 Entry of the toxic proteins abrin, modeccin, ricin, and diphtheria toxin into cells. I. Requirement for calcium.

7504 Entry of the toxic proteins abrin, modeccin, ricin, and diphtheria toxin into cells. 11. Effect of pH, metabolic inhibitors, and ionophores and evidence for toxin pen- etration from endocytotic vesicles.

Kirsten Sandvig and Sjur Olanes

Kirsten Sandtig and Sjur Olsnes

7514 Energetics of y-aminobutyrate transport in rat brain

Anna Pastuszko, Dacid F. Wilson, and Maria Erecin-

7520 Lectin from embryos and oocytes of Xenopus laevis.

Marie M. Noherson and Samuel H. Barondes 7541 Regulation of the phosphorylation and activity of 6-

phosphofructo 1-kinase in isolated hepatocytes by a- glycerolphosphate and fructose 2,6-bisphosphate.

Thornus H. Clnus, John R. Schlumpf. M. Rnafot E l - Maghmhl, nnd Simon J . Pilkis

synaptosomes.

siru

Purification and properties.

7566 Thyrotropin-releasing hormone rapidly and tran- siently stimulates cytosolic calcium-dependent pro- tein phosphorylation in GH:t pituitary cells.

Dehrn S. Drust and Thornus F. .J. Martin 7587 Metabolic compartments in Dictyostelium discoideum.

Borhnru E. Wright, D a c d A . Thomas. and Dehrn .J. fngnlis

7603 Regulation of rat liver fructose 2,6-bisphosphatase.

Eric. Fox-. and Simon J . Pilkls

the Golgi enzyme galactosyltransferase (lactose syn- thetase A protein) in HeLa cells.

M . Knafnt El-Maghrahi, Thornus H. Claus, JO Pilkrs,

7 162.3 .‘ . Biosynthesis, intracellular transport, and release of

Cer J. A . M. Strous and Eric C . Berger 7720 Chemical characterization of a neural cell adhesion

molecule purified from embryonic brain membranes. Stunley Hoffnlan, Barhnrn C. Sorkin, Perrin C. White,

Robert Brnckenhury, Kemhnrd Muilharnmer, lirs Hutis- hauser, Bruce A . Cunningham. tend Gerald M. Is’delmnn

7814 Normalization of intracellular lysosomal hydrolases in I-cell disease fibroblasts with sucrose loading.

KoJi Inur. Tohru Yutaka, and HynkuJi Yubuccchi Tomochika Kato, Shintnro Okadu. Toshw Ohshlma.

ENZYMOLOGY -7294 Dopamine-derived tetrahydroisoquinolines. Novel in-

Rong-sen Shen, Robert V. Smith, Putrick J , Lkcc~~ . s ,

-7298 Phosphotyrosyl-protein phosphatase of TCRC-2 cells. Ghunshyccm Stcvsrup. K. L’rncent Speeg, Jr.. Strcnltjy

hibitors of dihydropteridine reductase.

Ahrnm Rruhnhw. and Creed Ahell

Cohen, crnd Ducwf L. Gnrhers -7306 Purification of a myosin phosphatase from bovine

D i m e K. Werth, cJoe K. Hueherlc~. nncl D a c ~ t i K. Huth- aortic smooth muscle.

ntcYly

Indicates a Communication

6

7348

7360

7422

5460

7468

7485

7531

7549

7595

7615

7646

7650

7684

Inhibition of milk xanthine oxidase by fluorodinitro- benzene.

Takeshi Nishino. Keizo Tsushima, Kuss Hille, and Vin- cent Massey

Kinetics of the heparin-enhanced antithrombin III/

the mechanism of action of heparin. thrombin reaction. Evidence for a template model for

Michael J . Griffith

Isolation and characterization of rat liver microsomal UDP-glucuronosyltransferase activity toward che- nodeoxycholic acid and testosterone as a single form of enzyme.

Gerok Heidrun Matern, Siegfried Matern, and Wolfgang

Purification and characterization of an a-ketoisocap- roate oxygenase of rat liver.

The mechanism of a-ketoisocaproate oxygenase. For- mation of B-hydroxyisovalerate from a-ketoisocap-

Patrick J. Sabourin and Loran L. Bieber.

. . roate.

Patrick J . Sabourin and Loran L. Bieber

Regulation of adenylate cyclase of human platelet membranes by forskolin.

Pnul A. Insel, Dommique Stengel. Nicolus Ferry, and Jacques Hanoune

Arylamine N-methyltransferase. Methylation of the indole ring.

boxylase in mouse kidney. Studies using labeling of Effect of androgens on turnover of ornithine decar-

the enzyme by reaction with [14C]a-difluoromethyl- ornithine.

James E . Secly, Hunnu Ptiso, and Anthon.)' E. Pegg

Hepatic pyruvate kinase. Quantitative measurements of phosphorylation in vitro and in the isolated rat

Ellen Sue Lyon a n d Willmm B. -Jakohy

hepatocyte. James B. Blair, Michele A. C'imhalu, and Mdinda E .

James

Subunit interaction in catalysis. Some experimental and theoretical approaches with glyceraldehyde-3- phosphate dehydrogenase.

Reactivity and metal-dependent stereospecificity of the phosphorothioate analogs of ATP in the arginine kinase reaction. Structure of the metal-nucleoside tri- phosphate substrate.

Reactivity and metal-dependent stereospecificity of the phosphorothioate analogs of ADP and ATP and reactivity of Cr(II1)ATP in the 3-phosphoglycerate kinase reaction. Structure of the metal nucleotide substrates.

A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deox- yadenosine triphosphate.

Jeffre-v W.. Cardon and Paul D. Boyer

Mildred Cohn. Nancy Shih. and Jerelyn Nlck

Etleen K. Jaffe, Jerelyn Nick, and Mddred Cohn

Paul J . Romcrniuh nnd Fritz fi,'clz.stein

7794

7803

7808

7820

784 7

5898

-7290

-73 18

7354

7366

7414

7435

7449

7554

758 1

A comparative study of heme degradation by NADPH-cytochrome c reductase alone and by the complete heme oxygenase system. Distinctive aspects of heme degradation by NADPH-cytochrome c reduc- tase.

Takeo Yoshinaga, Shigeru Sassa, and Attallah Kappas

The oxidative degradation of heme c by the micro- somal heme oxygenase system.

Takeo Yoshinaga, Shigeru Sassa. and Attallah Kappas Poly(ADP-ribosylation) in vitro. Reaction parameters and enzyme mechanism.

Action of mung bean nuclease on supercoiled PM2 DNA.

Direct activation of calcium-activated, phospholipid- dependent protein kinase by tumor-promoting phor-

Ari M. Ferro and Baldomero M. Olioera

Dmid Koulalski nnd Janet P. Sanford

bo1 esters. ~

mihiko Sano, i?shio Kikkaua, and Ynsutomi Nishizuka Monrque Castagna, Yoshimi Takai, Kozo Knibuchi, Ki-

The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

Paul B. Wolfe, Pamela SilL'er, and William Wickner

MEMBRANES AND BIOENERGETICS Cytochalasin B. A natural photoaffinity ligand for labeling the human erythrocyte glucose transporter.

Solubilization and localization of cholesteryl oleate in egg phosphatidylcholine vesicles. A carbon 13 NMR study.

The isolated y subunit of Escherichia coli FI ATPase binds the E subunit.

Stanley D. Dunn Direct fluorescence measurements of M$+ binding to sarcoplasmic reticulum ATPase.

Florent Guillnin, Murcel P. Gingold, a n d Philippe Champeil

man red blood cells. Vanadate-induced movements of Ca2+ and K + in hu-

Evidence for the organization of the transmembrane

embedded and surface domains of the a-subunit. segments of (Na,K)-ATPase based on labeling lipid-

Peter L. Jw-gensen, Steven J . D. Karlish, and Carlos Gitler

transport from NADH to cytochrome P-450. Kinetic investigation of rat liver microsomal electron

Reductive methylation of the two 4,4'-diisothiocyano- dihydrostilbene-2,2'-disulfonate-binding lysine resi- dues of band 3, the human erythrocyte anion trans- port protein.

Isolation and purification of collagen ul(1) receptor from human platelet membrane.

Michael F. Shnnahan

James A. Hamilton cmd Donald M. Small

Ludolit Vurec1:n and Ernesto Curafoli

Gary J . Fisher and James L. Gavlor

Michael L. Jennings

5689 Template-primer-dependent turnover of (S,)-dATPaS Thomas M. -Chiang and Andreu, H. Kang by T4 DNA polymerase. The stereochemistry of the 7608 Heterogeneity of rabbit intestine brush border associated 3' + 5'-exonuclease.

Anfar Gupta. Chnrles DeBrosse. and Stephen J . Ben- plasma membrane cholesterol.

BernahP Bloi and Donald B. Zilc~ersm~t

7737

".. ( 1 1 8

5786

/:otic Thymus myosin. Isolation and characterization of myosin from calf thymus and thymic lymphocytes, and studies on the effect of phosphorylation of its M . = 20,000 light chain.

Jonathan M. Scholey, Robin C. Smith, DetleLr Drenck- hahn, Ute Groschel-Stewart, and John Kendrick-Jones Purification and properties of bovine spleen heme oxygenase. Amino acid composition and sites of ac- tion of inhibitors of heme oxidation.

Taheo Yoshinaga, Shigeru Snssa. and Attnllah Kuppus The occurrence of molecular interactions among NADPH-cytochrome c reductase, heme oxygenase, and biliverdin reductase in heme degradation.

Takeo Yoshinuga, Shigeru Sassu, and Attnllah Knppas

7655 Phosphoenzyme formation from ATP in the ATPase of sarcoplasmic reticulum. Effect of KC1 or ATP and slow dissociation of ATP from precursor enzyme-ATP complex.

Munekazu Shigekntca und Tohru Kannzcru>ct 7704 Permeability of canine cardiac sarcoplasmic reticu-

lum vesicles to K+, N', H+, and C1- Gerhard Meissner and Dona McKinley

7762 Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley.

Guy Bellamare, Sue G . Bartlett, and Nam-Hai Chua 7879 Purification and polypeptide characterization of com.

plex 111 from yeast mitochondria. Anita Sidhu and Diana D. Beattie

Indicates a Communication

7

PROTEIN CHEMISTRY AND STRUCTURE

Genetic variation in glycosylation of the fourth com- ponent of murine complement. Association with he- molytic activity.

Daoid R. Karp, John P. Atkinson, and Donald C. Shref-

Study of the structure of troponin-I by measuring the relative reactivities of lysines with acetic anhydride.

fler

Sarah E . Hitchcock-De Gregori Arrangement of attachment-promoting, self-associa- tion, and heparin-binding sites in horse serum fibro- nectin.

Ruth Ehrismann, Donna E . Roth, Hans M. Eppenher- ger, and Dauid C. Turner Structure of the w-aminocarboxylic acid-binding sites of human plasminogen. Arginine 70 and aspartic acid 56 are essential for binding of ligand by Kringle 4.

residues on the kinetics of polymerization of deoxy- Effect of cross-linking between 82Pt and 82p2 lysyl

Maria Trexler, Zsofia Vuli, and Laszlo Patthy

-7277

-7280

732’2

7336

7388

7629

7712

7730

7746

7756

7826

7834

7857

7887

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

Coordinate accumulation of the mRNAs for the a, P , and chains of rat fibrinogen following defibrination.

DNA primase activity from human lymphocytes. Syn- thesis of oligoribonucleotides that prime DNA synthe-

Gerald R. Crabtree and Jeffrey A . Kant

SIS. Ben Y. Tseng and Clarence N. Ahlem

Nucleotide sequences of switch regions of immuno- globulin C, and C, genes and their comparison.

Toshio Nikaido, Yuriko Yamawakl-Kataoka, and Ta- suku Honjo Histone acetylation increases the solubility of chro- matin and occurs sequentially over most of the chro- matin. A novel model for the biological role of histone acetylation.

A thermostable tRNA (guanosine-2’-)-methyltrans- ferase from Thermus thermophilus HB27 and the ef- fect of ribose methylation on the conformational sta- bility of tRNA.

ima Zzumi Kumagai, Kirnitsuna Watanabe, and Tairo Osh-

Rapid changes in the concentration of phosphoenol-

ney. Effects of insulin and cyclic AMP. pyruvate carboxykinase mRNA in rat liver and kid-

Michele A . Cimbala, Wouter H. Larners, Kathleen Nel- son, John E. Monahan, Heeja Yoo- Warren, and Richard W. Hanson Eukaryotic ribosomal subunit anti-association activ- ity of calf liver is contained in a single polypeptide chain protein of M , = 25,500 (eukaryotic initiation factor 6).

Maitra D a c d M. Valenzuela, Asok Chaudhuri, and Umadas

Chromatin structure of the chicken &globin gene re-

and DNase II. gion. Sensitivity to DNase I, micrococcal nuclease,

Characterization of Nicotiana tabucum chloroplast and cytoplasmic ribosomal proteins.

Malcolm S. Cupel and Don P. Boryue The molecular characterization of three transcrip- tional mutations in the yeast iso-2-cytochrome c gene.

Donna L. Montgomery, Jeremy M. Boss, Stephen J. McAndrew, Linda Marr, Deborah A. Walthall, and Rich- ard S. Zitomer Structure of the Syrian hamster ribosomal DNA re- peat and identification of homologous and nonhomol- ogous regions shared by human and hamster ribo-

Michael Perry and Roger Chalkley

Willtam I. Wood and Gary Felsenfeld

sGmaI DNAS.

Hart, Kathleen L. Berkner, and William R. Folk Steven J . Triezenberg, Caroline Rushford, Ronald P.

Pretranslational regulation of a2,-globulin in rat liver by growth hormone.

T. Surend Nath, and Nalini M. Motuani Arun K. Roy, Bandana Chatterjee, William F. Demyan,

Transcription and precursor processing of normal and mutant human tRNAi“‘ genes in a homologous cell-free system.

Michael Zaasloff, Tomas Santos, Paul Romeo, and Mar- tin Rosenberg Structure of amplified normal and variant dihydro- folate reductase genes in mouse sarcoma S180 cells.

Gray F. Crouse, Christian C. Sirnonsen, Robert N . McEuwz, and Hobert T. Schzrnke

Indicates a Communication GENERAL

Submit all manuscripts in triplicate to Editor, The Journal of Biological Chemistry

Bethesda, MD 20814, USA. 9650 Rockville Pike

standing that the author(s) will pay a charge per page (miniprint Accepted manuscripts will be published with the implicit under-

supplement excluded). Current page charges may be obtained by contacting the JBC Office. LJnder exceptional circumstances, when no source of grant or other support exists, the author(s) may apply, a t the time of submission, for a grant-in-aid to Chairman, Publi- cations Committee. American Societv of Biological Chemists, Inc.,

7330

7372

7381

7401

7525

7536

7560

7574

7672

7678

7768

7839

7852

7864

7903

hemoglobin S.

chi

~~

Kiyomi Kikugaura, Toshio Asakura, and Karuhiko Ada-

Structure of mouse submaxillary gland renin. Identi- fication of two disulfide-linked polypeptide chains and the complete amino acid sequence of the light chain.

The exchange of Fe3’ between acetohydroxamic acid and transferrin. Spectrophotometric evidence for a mixed ligand complex.

Bates Richard R. Cowart, Nakao Kojima, and George W.

Purification and macromolecular properties of a sialic acid specific lectin from the slug L i m a fi’uuus.

Fish Ronald L. Miller, James F. Pollawn, Jr., and Wayne W.

Iron binding by horse spleen apoferritin. A vanadyl (IV) EPR spin probe study.

A structural role for the Ca2+-M&+ sites on troponin C in the regulation of muscle contraction. Preparation and properties of troponin C depleted myofibrils.

roperoxidase. Crystallization and preliminary x-ray data for chlo-

Byron Rubin, Jody VanMiddlesworth, Kenneth Thomas, and Lowell Huger

of H-9-encoded proteins on target cell plasma mem- Physical separation and biochemical characterization

branes and endoplasmic reticulum.

collagens with human type V collagen. Human cartilage collagens. Comparison of cartilage

Robert E. Burgeson, Patricia A . Hebda, Nicholas P. Morris, and Darid W. Hollister Maturation of the tail spike endorhamnosidase of Sal- monella phage P22.

David P. Goldenberg, Peter B. Berget, and Jonathan King In vitro translation and processing of a precursor form of favin, a lectin from Vicia fubu.

John J. Hemperly, Keith E. Mostor: and Bruce A. Cun- ningham

Kunio S. Misono and Tadash1 Inagami

N. Dennis Chasteen and Elizabeth C. Theil

Henry G. Zot and James D. Potter

Penny J . Gilmer

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The Journal ofBiological Chemistry publishes papers on a broad range of topics of interest to biochemists. The views expressed are

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