vizilite_vscope 3-08 copy
DESCRIPTION
dentalTRANSCRIPT
-
1What about new waysof detecting potentially
malignant lesionsearlier?
Critical Evaluation of Diagnostic Aids forthe Detection of Oral Cancer*
M Lingen, J Kalmar, T Karrison & P Speight
Oral Oncology 2008 Jan;44(1):10-22University of Chicago, Ohio State University, University of Sheffield
* A position paper of theAmerican Academy of Oral and Maxillofacial Pathology
True Positive and TrueNegative Requires a
GOLD STANDARDGOLD STANDARD for
tissue diagnosis is ascalpel biopsy
Screening vs. Case-FindingCase-finding - a diagnostic test fora patient who has abnormal signs orsymptoms (Class I)
Screening - checking for a disease in aperson who is symptom free (Class II)
Evidence ofchemiluminescenceenhancing intraoral
examinations?Lets look at the scientific
literature
-
2Evidence of chemiluminescenceenhancing intraoral
examinations?Literature was only for
gynecologicalexaminations until 2004
Acetic Acid Wash and Chemiluminescent Illumination asan Adjunct to Conventional Oral Soft Tissue Examination
for the Detection of Dysplasia: A Pilot StudyHuber MA, Bsoul SA, Terezhalmy GT
Quintessence International 2004: 35(5):378-84
150 consecutive patients Examined linea alba, leukoedema, hairy tongue, leukoplakia, traumatic
ulcer, fibroma, amalgam tattoo, tori and frictional keratosis All 32 cases of leukoedema were positive (aceto-white) - False Positives 2 of 14 frictional keratoses were positive; No biopsies performed on
frictional keratoses 3 Leukoplakias
1 biopsied = hyperkeratosis2 brushed, 1 atypical biopsied = ulcer
One lesion aceto-white lesion identified not clinically apparent
Strengths of this study Large sample size Consecutive patients One lesion detected by ViziLite (not stated what diagnosis was)
Weaknesses of this study
Few cases with histologic correlation Cant assess sensitivity, specificity or predictive value High false positives - suggests that sensitivity may behigh but specificity and PPV are low
Chemiluminescence as a diagnostic aid in the detection oforal cancer and potentially malignant epithelial lesions
Ram, S. and Siar, C.H.Int J Oral maxillofac Surg, 2005;34: 521-527
Study was of already discovered lesions and 5 cases of normaloral mucosa
Comparison to 1% tolonium chloride (toluidine blue) mouthrinse
Negatives were not biopsiedHigh sensitivity, very low specificity (14.2% Vizilite and 25%
tolonium chloride)
This was not a study of efficacy for using Visilite as a screeningmethod
Evidence of chemiluminescence enhancingintraoral examinations?
In our study we have examined only overtly obviouscases of oral cancer which do not require specialscreening tests for identification. In this respect, wehave not been able to detect any case of early oralcancer (CIN) in normal-looking oral mucosa with thistool. The other shortcoming is the observed falsepositive rate of 6/31 lesions indicating the Vizilite isnon-specific and likely to result in many unnecessarybiopsies
S. Ram and C.H. SiarFaculty of Dentistry
University of Malaya
The efficacy of oral lumenoscopy (ViziLite) invisualizing oral mucosal lesions.
Epstein JB, Gorsky M, Lonky S, Silverman S Jr,Epstein JD, Bride M.
Special Care Dentist 2006 Jul-Aug;26(4):171-4College of Dentistry and Illinois Cancer Center, University of Illinois
-
3All patients had history of previously-detected oralmucosal lesions - Not screening
2 occult lesions in separate patients with previous hx ofsquamous cell carcinoma - one recurrent Ca, one benign
The chemiluminescent light did not appear to improvevisualization of red lesions, but white lesions and lesionsthat were both red and white showed enhancedbrightness and sharpness.
Conclusion:ViziLite examination did notsignificantly improve lesiondetection compared to COE
Weakness:Lack of standardized correlation ofclinical findings to histopathologic
diagnosis of lesional tissue
Clinical evaluation ofchemiluminescent lighting: an adjunct
for oral mucosal examinationsKerr AR, Sirois DA, Epstein JB.
J Clin Dent2006; 17(3):59-63
The Bluestone Center for Clinical Research, NewYork University College of Dentistry
Aim: Describe the utility of Vizilite as aadjunct to visual examination, especially those
lesions clinically suspicious forpre-cancer or cancer
501 patients with positive tobacco history410 suspicious lesions in 270 patients by visualexamination [suspicious for WHAT?]127 lesions suspicious for oral cancer98 were aceto-white + 6 not seen visually77 (78.5%) were suspicious [what happened to 50suspicious lesions?]
ResultsSuggest that Vizilite, when used as a screeningadjunct provides additional visual informationLeukoplakias may be more readily observedOnly sharpness was significantly improvedStudies are underway to explore the clinicalsignificance and predictive value of oralchemiluminescence lighting
NO BIOPSIES WERE PERFORMEDTHIS IS NO HELP WHATSOEVER -
NO IDEA WHAT THE 6 LESIONS DETECTED BYVIZILITE WERE
A pilot case control study on the efficacy of aceticacid wash and chemiluminescent illumination
(ViziLite) in the visualisation of oral mucosalwhite lesions
Camile S. Farah, and Michael J. McCulloughOral Oncology 2007 Sep;43(8):820-4
Department of Oral Medicine and Pathology, School of Dentistry, TheUniversity of Queensland, Brisbane QLD 4072, Australia
School of Dental Science, The University of Melbourne, Melbourne VIC3000, Australia
Received 12 September 2006; revised 17 October 2006; accepted 18October 2006; on-line pub. Dec. 2006
-
4Strengths:All lesions were biopsied
Weaknesses:2 diagnostic specialists performed all
examinationsCouldnt directly compare clinicaland histological findings for specific
lesions
Aim: Assess efficacy in visualization of oralmucosal white lesions in a tertiary referral center55 pts referred for existent white lesions [not screening]Vizilite subjectively enhanced visualization in 26/55No significant difference in lesion SIZE, EASE OF VISIBILITY, ORBORDER DISTINCTIVENESSCould not distinguish between epithelial hyperplasia, dysplasia, carcinomaor inflammationAll appeared aceto-whiteDid not change provisional diagnosis, nor alter biopsy site
Sensitivity 100%Specificity 0%Accuracy 18.2%
Sensitivity # of true positive results divided by the number of those who have the disease
Specificity # of true negative results divided by the number of
those who are without the disease
Depends on a comparison to a goldstandard
Positive Predictive Value# of true positive results divided by the number of those who tested to have the disease
Negative Predictive Value# of true negative results divided by the number ofthose who tested to be without the disease
Depends on a comparison to a goldstandard Conclusions(Farah and McCullough)
Vizilite does not aid in identification of malignantand potentially malignant oral lesionsDoes not discriminate between keratosis,inflammation, malignant or potentially malignantlesionsLittle if any benefit for lesions already detectedHigh index of suspicion, expert clinical judgementand scalpel biopsy is still essential for proper patientcare
-
5Efficacy of the ViziLite System in theIdentification of Oral Lesions
Oh, ES and Laskin, DM
J Oral Maxillofac Surg 2007 Mar;65(3):424-6School of Dentistry, University of North Carolina, Chapel Hill, NC
100 patients39 males
61 femalesOral exam with incandescent light - 29 clinically
undiagnosable lesions
Oral exam with acetic acid rinse - 3 additionalclinically undiagnosable lesions
Oral exam with ViziLite - no additional clinicallyundiagnosable lesions
32 clinically undiagnosable lesions were examined withOral CDx
2 were atypical and were biopsiedNeither was premalignant or malignant
Conclusions: Although the acid rinseaccentuated some lesions, the overall detection
rate was not significantly improved. Thechemiluminescent light produced reflections
that made visualization more difficult and thuswas not beneficial.
32 clinically undiagnosable lesions were examined withOral CDx
2 were atypical and were biopsiedNeither was premalignant or malignant
Conclusions: Although the acid rinseaccentuated some lesions, the overall detection
rate was not significantly improved. Thechemiluminescent light produced reflections
that made visualization more difficult and thuswas not beneficial.
Vizilite My opinion at this time
Extra time and care using the systemARE VALUABLE
Evidence of aiding detection of premalignantlesions is quite sparse
Unclear what added benefit there is topractitioner
Cannot discriminate indolent lesions frombiologically worrisome
Not worth charging the patient extra money over astandard visual and tactile exam
Vizilite is NOT approved by the ADA Council on Scientific AffairsIf you do the Vizilite exam, how would you justify charging your patientbased on peer reviewed evidence?
Well-controlled clinical trials are needed that specifically investigate theability of ViziLite to detect precancerous lesions that are invisible by COEalone. Studies needed for combined ViziLite and toluidine blue dye.If such discrimination can be confirmed, it would support the use of thistechnology as a true screening device
Vizilite My opinion at this time(2)
-
6VELscopeA Magic Wand for the Community Dental Office?
Observations from theBritish Columbia Oral CancerPrevention Program
Laronde D, Poh C, Williams P, Hislop T, Zhang L, MacAulay C, and RosinM.
J Canadian Dent Assoc Sept 2007 (73)7; 607-609
Loss of fluorescence occurs in many benign mucosal conditions, such asgeographic tongue, aphthous ulcers (sic) and tissue trauma.Training in communication skills directed at reducing patient anxietyrelated to abnormal screening result is also important.
VELscopeThe use of the device encouraged greater attention to oral cancer screeningin their practices, leading to the identification of lesions that may have beenotherwise overlooked.
ConclusionsFV has the potential to be an adjunctive screening tool that facilitates thediscrimination of soft tissue changes requiring follow-up.It will not replace conventional clinical examination.Although evidence supports its use in high-risk clinics, its value in generalpractice remains to be determined.No current evidence that using FV in general dental practice saves lives, butregular use of a high quality screening examination could make a difference.
VELscopeEvidence-Based Decision-Making: Should the General
Dentist Adopt the Use of the VELscope for RoutineScreening for Oral Cancer?
Balevi BJ Canadian Dent Assoc Sept 2007 (73)7; 603-606
4 clinical papers reviewed and critically appraised, 2 case studies, 2observational studiesAll studies included patients seen in referral clinics that are specialized inthe diagnosis and management of oral pathologyRisk of test-referral bias
VELscope2 case studies demonstrated potential benefit during follow-up of alreadydiagnosed (high-risk) patients to check for new lesions2 observational studies may be giving an artificially high true-positive rate(sensitivity) and true-negative rate (specificity) because of spectrum biasNo evidence this device can distinguish between oral cancer and aphthousulcers (sic), lichen planus, pemphigoid, etc.No long-term evidence that this device actually saves livesIt is important to consider the potential risk of harm from a false-positivereadingNo assessment of inter-observer and intra-observer agreement
VELscopeShortcomings
Currently, neither the scientific evidence nor the levelof clinical skill justifies the routine us of the VELscopein a general dental practiceThe device is more likely to detect the more commonabnormal oral tissue lesions than oral cancerNo intra- and inter-operator agreement has beenverified
VELscopeConclusions
No evidence that routine use saves livesConcern about the risk of harm if routinelyusedRoutine use is prematureVELscope may be of value in a clinic that isspecialized in management of oral cancer
-
7VELscopeMy opinion at this time
Best use is in determining margins for excisionsof premalignant lesionsCould be useful in screening if shortcomingscited in the article can be corrected A cell-based sensor to detect oral cancer biomarkers,
such as the epidermal growth factor receptor (EGFR)whose over-expression is associated with early oraltumorigenesis and aggressive cancer phenotypes.
Cell-based sensor for analysis of EGFRbiomarker expression in oral cancer
Shannon E. Weigum, Pierre N. Floriano, NicolaosChristodoulides and John T. McDevitt
Lab Chip, 2007, 7, 995 - 1003
The lab-on-a-chip (LOC) sensor utilizes an embedded track-etched membrane, which functions as a micro-sieve, to captureand enrich cells from complex biological fluids or biopsysuspensions.
Immunofluorescent assays reveal the presence and isotype ofinterrogated cells via automated microscopy and fluorescentimage analysis.
Using the LOC sensor system, with integrated capture andstaining technique, EGFR assays were completed in less than10 minutes with staining intensity, homogeneity, and cellularlocalization patterns comparable to conventional labelingmethods.
These results support the LOC sensor system as a suitableplatform for rapid detection of oral cancer biomarkers andcharacterization of EGFR over-expression in oral malignancies.Application of this technique may be clinically useful in cancerdiagnostics for early detection, prognostic evaluation, andtherapeutic selection
Examples of what you can do with the LOC
* Real-time PCR ;detect bacteria, viruses and cancers. * Immunoassay ; detect bacteria, viruses and cancers based
on antigen-antibody reactions. * Dielectrophoresis detecting cancer cells and bacteria. * Blood sample preparation ; can crack cells to extract
DNA. * Cellular lab-on-a-chip for single-cell analysis. * Ion channel screening
Salivary diagnostics for oral cancer David T. Wong
J Calif Dent Assoc. 2006 Apr;34(4):303-8two salivary proteins, interleukin (IL)-8 and thioredoxin, that
can discriminate between saliva of oral cancer and controlsubjects.
IL-8 is significantly elevated in saliva of oral cancer patientsand is highly discriminatory of detecting oral cancer in saliva
(n = 64) with an receiver operator characteristic (ROC) value of0.95, sensitivity 86% and specificity 97%
Other results suggested that saliva thioredoxin is a validatedbiomarker for oral cancer detection
-
8UCLA Oral Fluid Nanosensor
Biopsy versusother methods of
analyzing oraltissues
The Brush Test is acase-finding test, not
a screening test
Brush Testexfoliative cytology
PROS No anesthesia Patient
acceptance
CONS Negative results are not
biopsied ? trust ? No research on negatives Negative results were not
tested against the goldstandard
Brush Testexfoliative cytology
PROS No anesthesia Patient
acceptance
CONS(continued) No diagnosis Positive or Atypical
results still need to bebiopsied delayed diagnosis extra cost
Brush Testexfoliative cytology
PROS No anesthesia Patient
acceptance
CONS(continued) Extremely high number
of false positives
-
9Brush Testexfoliative cytology
PROS No anesthesia Patient
acceptance
CONS(continued) No ability to detect
dysplasia byarchitecture
No baseline histology iffuture biopsies are done
Brush Testexfoliative cytology
ADA Council on Scientific Affairs
All Oral CDx atypical or positiveresults must be confirmed by incisional
biopsy and histology to completelycharacterize the lesion.
Brush Testexfoliative cytology
ADA Council on Scientific Affairs
Persistent lesions, even with negativeresults, must receive adequate
follow-up evaluations.
Brush Testexfoliative cytology
ADA Council on Scientific Affairs
Persistent lesions, even with negativeresults, must receive adequate
follow-up evaluations.
Brush Testexfoliative cytology
ADA Council on Scientific Affairs
Persistent lesions, even with negativeresults, must receive adequate
follow-up evaluations.????????????
Brush Testexfoliative cytology
Shows promiseStudy needed with a large cohort of Class II subjects with
brush and biopsy of ALL subjectsBrush Test may be beneficial:
1. Multiple lesions, particularly in someone with nohistory of oral cancer2. Non-compliant patient who is unlikely to come back fora follow-up exam or accept an immediate biopsyJudicious use in these scenarios may be clinically useful