VirusesViruses

What is a virus?https://www.youtube.com/watch?v=67ays2ZYr48

You've probably had the flu-influenza-at some time during your life. Nonliving particles called viruses cause influenza. Viruses are composed of nucleic acids enclosed in a protein coat and are smaller than the smallest bacterium. Viruses are considered to be nonliving because they don’t exhibit all the criteria for life. They don’t carry out reparation, grow or develop. All viruses can do is replicate-make copies of themselves-and they can’t even do that without the help of living cells. A cell in which a virus replicates is called the host cell.

Because viruses are non living, viruses were not named in the same way as organisms. Viruses, such as rabies viruses and polioviruses, were named after the disease they cause. Other viruses were named for the organ or tissue they infect. For example, scientists fires fount eh adenovirus which is one cause of the common cold, in adenoid tissue between the back of the throat and the nasal cavity. https://www.youtube.com/watch?v=oqGuJhOeMekhttps://www.youtube.com/watch?v=oqGuJhOeMekhttps://www.youtube.com/watch?v=oqGuJhOeMek

Today most viruses are given a genus name ending in the word “virus” and a species name. however, sometimes scientists use code numbers for distinguish among similar viruses that infect the common intestinal bacteria, Escherichia coli, have the code number T1 through T7 (T stands for “Type”). A virus that infects a bacterium is called a bacteriophage or phage for short.

Viral StructureA virus has an inner core of nucleic acid, either RNA or DNA , and an outer protein coat called a capsid. Some relatively large viruses, such as human flu viruses, many have an additional layer, called an envelope, surrounding their capsids. Envelopes are composed primarily of the same materials found in the plasma of membranes of all cells. https://www.youtube.com/watch?v=f-ApyHtAONU

The core of nucleic acid contains a virus’s genetic material. Viral nucleic acid is either DNA or RNA and contains instructions for making copies of the virus. Some viruses have only four genes, while others have hundreds. The arrangement of proteins in the capsid of a virus determines the virus’s shape. https://www.youtube.com/watch?v=0Elo-zX1k8M

There are four different viral shapes. The protein arrangement also plays a role in determining what cell can be infected and how the virus infects the cell.

https://www.youtube.com/watch?v=05bwTTWlIGA

Virus Shapes

Attachment to a hostBefore a virus can replicate, it must enter a host cell.

Before it can enter, it must first recognize and attach to a receptor site on the plasma membrane of the host cell. https://www.youtube.com/watch?v=Rpj0emEGShQ

A virus recognizes and attaches to a host cell when one of its proteins interlocks with a molecular shape that is the receptor site on the host cell’s plasma membrane.

A protein in the tail fibers of the bacteriophage, T4 recognizes and attaches the T4 to its bacterial host cell. In other viruses, the attachment protein is in the capsid or in the envelope. The recognition and attachment process is like two pieces of a jigsaw puzzle fitting together. The process might also remind you of two spaceships docking.

https://www.youtube.com/watch?v=RO8MP3wMvqg

Attachment is a specific processEach virus has a specifically shaped attachment protein. Therefore, each virus

can usually attach to only a few kinds of cells. For example, the T4 phage can infect only certain types of E.Coli because the

T4’s attachment protein matches a surface molecules of only these E.Coli. A T4 cannot infect a human, animal, or plant cell, or even another bacterium. In general, viruses are species specific and some also are cell-type specific. For example polio viruses normally infect only intestinal and nerve cells.

The species specific characteristic of viruses is significant for controlling the spread of viral diseases. For example, by 1980, the World Health Organization had announced that smallpox, which is a deadly human viral disease, had been eradicated. The eradication was possible partly because the small pox virus infects only humans. A virus such as the one that causes the flu is not species specific and infects animals as well as humans; therefore, it is difficult to eradicate. A virus such as West Nile virus infects mainly birds, horses and humans.

Viral Replication CyclesViral Replication Cycles

Once attached to the plasma membrane of the host cell, the virus enters the cell and takes over its metabolism. Only then can the virus replicate. Viruses have two ways of getting into the host cells. The virus may inject its nucleic acid into the host cell like a syringe injects a vaccine into your arm. The capsid of the virus stays attached to the outside of the host cell.

An enveloped virus enters a host cell in a different way. After attachment, the plasma membrane of the host cell surrounds the virus and produces a virus-filled vacuole inside the host cell’s cytoplasm. Then, the virus bursts out of the vacuole and release its nucleic acid into the cell.

Lytic CycleOnce inside the host cell, a virus’s genes are

expressed and the substances that are produced take over the host cell’s genetic material. The viral genes alter the host cell to make new viruses. The host cell uses its own enzymes, raw materials, and energy to make copies of viral genes that along with viral proteins are assembled into new viruses, which burst from the host cell, killing it. The new viruses can then infect and kill other host cells. This process is called a lytic cycle.

Lysogenic CycleNot all viruses kill the cells they infect. Some viruses go through a

lysogenic cycle, a replication cycle in which the virus's nucleic acid is integrated into the host cell’s chromosome.

A lysogenic cycle begins in the same way as a lytic cycle. The virus attaches to the host cell’s plasma membrane and its nucleic acid enters the cell. However, in a lysogenic cycle, instead of immediately taking over the host’s genetic material, the viral DNA is integrated into the host cell’s chromosome.

Viral DNA that is integrated into the host cell’s chromosome is called a provirus. A provirus may not affect the functioning of its host cell, which continues to carry out its own metabolic activity. However, every time the host cell reproduces, the provirus is replicated along with the host cell’s chromosomes.

Therefore, every cell that originates from an infected host cell has a copy of the provirus. The lysogentic phase can continue for many years. However, at anytime, the provirus can be activated and enter a lytic cycle. Then the virus replicates and kills the host cell.

Disease Symptoms of ProvirusesThe lysogenic process explains the reoccurrence of

cold sores, which are caused by the Herpes simplex I virus. Even though a cold sore heals, the herpes simplex I virus remains in your cells as a provirus.

When the provirus enters a lytic cycle, another cold sore erupts. No one knows what causes a provirus to be activated, but some scientists suspect that physical stress, such as sunburn, and emotional stress, such as anxiety, play a role.

Many disease-causing viruses have lysogenic cycles. Three examples of these viruses are herpes simplex I, herpes simplex II that causes genital herpes, and the hepatitis B virus that causes hepatitis B. Another lysogenic virus is the one that causes chicken pox. Having chicken pox, which usually occurs before age ten, gives lifelong protection from another infection by the virus. However, some chicken pox viruses may remain as provirus's in some of your body's nerve cells. Later in your life, these provirus's may enter a lytic cycle and cause a disease called shingles.

Release of Viruses Release of Viruses Either Either lytic, the bursting of a celllytic, the bursting of a cell, or , or exocytosisexocytosis the the

active transport process active transport process by which by which materials are materials are expelled from a cell, releases new viruses from the expelled from a cell, releases new viruses from the host cellhost cell..

In In exocytosisexocytosis, a , a newly produced virus approachesnewly produced virus approaches the the inner surface of the host cell’s inner surface of the host cell’s plasma membraneplasma membrane. . The plasma The plasma membranemembrane surroundssurrounds the virus, the virus, enclosingenclosing it in a vacuole that then it in a vacuole that then fuses with the fuses with the host cell’s host cell’s plasma membrane. Then, the plasma membrane. Then, the viruses are viruses are released to the outsidereleased to the outside. .

RetrovirusA virus that is composed not of DNA

but of RNA. Retroviruses have an enzyme, called reverse transcriptase, that gives them the unique property of transcribing their RNA into DNA after entering a cell. The retroviral DNA can then integrate into the chromosomal DNA of the host cell, to be expressed there. HIV is a retrovirus.

Once inside a host cellOnce inside a host cell, the retrovirus makes DNA , the retrovirus makes DNA from its RNA. To do this, it uses reverse from its RNA. To do this, it uses reverse transcriptase, an enzyme it carries inside its transcriptase, an enzyme it carries inside its capsid. This enzyme helps produce double-capsid. This enzyme helps produce double-stranded DNA from the viral RNA. Then the stranded DNA from the viral RNA. Then the double-stranded viral DNA is integrated into the double-stranded viral DNA is integrated into the host cell’s chromosome and host cell’s chromosome and becomes abecomes a provirusprovirus. . If reverse transcriptase is found in a person, it is If reverse transcriptase is found in a person, it is evidence for infection by a retrovirus. evidence for infection by a retrovirus.

HIV: An Infection of White Blood CellsHIV: An Infection of White Blood CellsOnce inside a human host, Once inside a human host, HIV infects white blood HIV infects white blood

cellscells. . Newly made viruses are released Newly made viruses are released into the into the blood stream blood stream by exocsytosis by exocsytosis and infect other white and infect other white blood cells. Infected host cells still function blood cells. Infected host cells still function normally because the viral genetic material is a normally because the viral genetic material is a provirus that produces only a small number of new provirus that produces only a small number of new viruses at a time. viruses at a time. Because the infected cells are still Because the infected cells are still able to function normally, an infected person may able to function normally, an infected person may not appear sicknot appear sick, but they can still transmit the virus , but they can still transmit the virus in their body fluid. in their body fluid.

An HIV-infected person can experience no AIDS symptoms for a long time. However, most people with an HIV infection eventually get AIDS because, over time, more white blood cells are infected and produce new viruses.

People gradually lose white blood cells because provirus's enter a lytic cycle and kill their host cells. Because white blood cells are part of the body’s disease-fighting system, their destruction interferes with the body’s ability to protect itself from organisms that cause disease, a symptom of AIDS.

Cancer and VirusesSome viruses have been linked

to certain cancers in humans and animals. For example, the hepatitis B virus has been shown to play a role in causing liver cancer. These viruses disrupt the normal growth and division of cells in a host, causing abnormal growth and creating tumors.

Prions and VirodsPrions and VirodsResearchers have recently discovered some Researchers have recently discovered some

particles that behave some what like viruses and particles that behave some what like viruses and causes infectious disease. causes infectious disease. Prions are composed of Prions are composed of proteins but have no nucleic acid to carry genetic proteins but have no nucleic acid to carry genetic informationinformation. Prions are thought to act by . Prions are thought to act by causingcausing other other proteinsproteins to to fold themselves incorrectlyfold themselves incorrectly, , resulting in improper functioning. Prions are resulting in improper functioning. Prions are responsibleresponsible for many animals diseasefor many animals disease, such as , such as mad cow disease and its human equivalent, mad cow disease and its human equivalent, Creutzfeldt Jakob Disease. Creutzfeldt Jakob Disease.

Viroids are composed of a single circular strand Viroids are composed of a single circular strand of RNA with no protein coatof RNA with no protein coat. Viroids have . Viroids have been shown to been shown to cause infectious disease cause infectious disease in in several several plantsplants. The amount of viroid RNA is . The amount of viroid RNA is much less than the amount found in viruses. much less than the amount found in viruses.

Plant VirusesThe first virus to be identified was a plant

virus, called tobacco mosaic virus, that caused disease in tobacco plants. There are more than 400 viruses that infect a variety of plants. These viruses cause as many as 1000 plant disease and are named according to their host plants. Plant viruses require wounds or insect bites to enter and infect a host, and do not use surface recognition. They do not undergo lytic or lysogennic phases.

Not all viral plant disease are fatal or even harmful. Some mosaic viruses cause striking patterns of color in the flowers of plants. The infected flowers, have streaks of vibrant, contrasting colors in their petals. These viruses are easily spread among plants when you cut an infected stem and then cut healthy stems with the same tool.

Origin of Viruses

You might assume that viruses represent an ancestral form of life because of their relatively uncomplicated structure. This is probably not so. For replication, viruses need host cells; therefore, scientists suggest that viruses might have originated from their host cells. Some scientists suggest that viruses are nucleic acids that break free from their host cells while maintaining an ability to replicate parasitically within the host cell.