virginia igem workshop #4 high school education series

Virginia iGEMWorkshop #4High School Education Series

Workshop Plan


• Lesson: PCR, Gel Electrophoresis, & Transformation

• Activity: Make Your Own Primers


Polymerase Chain Reaction


Polymerase Chain Reaction

3D PCR Animation:

http://www.dnalc.org/view/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation-with-no-audio.html


Primer Design


Gel Electrophoresis


Sequencing

Sanger Sequencing Explanation:

http://www.dnalc.org/resources/3d/29-sanger-sequencing.html


Transformation

Activity: Make Your Own Primers


DON’T FORGET! •18-22 base pairs•50-60% GC Content•Wallace Rule: 4 degrees per C or G, 2 degrees per A or T Melting temperature of the primer. Subtract two degrees for annealing temperature.•Melting temperature 55-60 degrees•3’ Nucleotide = C or G

NCBI Primer Blast: http://www.ncbi.nlm.nih.gov/tools/primer-blast/Primer3: http://frodo.wi.mit.edu/

Any Questions?


virginia igem workshop #4 high school education series

Documents

primers virginia igem

d pcr animation

transformation activity

t melting temperature

annealing temperature

g ncbi primer blast

gc content wallace rule

base pairs