vce vet laboratory skills · 3 2018 vet lab skills exam section a continued turn over question 5 a...

VCE VET LABORATORY SKILLSWritten examination

Wednesday 21 November 2018 Reading time: 9.00 am to 9.15 am (15 minutes) Writing time: 9.15 am to 10.45 am (1 hour 30 minutes)

QUESTION AND ANSWER BOOK

Structure of bookSection Number of

questionsNumber of questions

to be answeredNumber of

marks

A 20 20 20B 17 17 80

Total 100

• Studentsarepermittedtobringintotheexaminationroom:pens,pencils,highlighters,erasers,sharpeners,rulersandonescientificcalculator.

• StudentsareNOTpermittedtobringintotheexaminationroom:blanksheetsofpaperand/orcorrectionfluid/tape.

Materials supplied• Questionandanswerbookof19pages• Answersheetformultiple-choicequestions

Instructions• Writeyourstudent numberinthespaceprovidedaboveonthispage.• Checkthatyournameandstudent numberasprintedonyouranswersheetformultiple-choice

questionsarecorrect,andsignyournameinthespaceprovidedtoverifythis.• AllwrittenresponsesmustbeinEnglish.

At the end of the examination• Placetheanswersheetformultiple-choicequestionsinsidethefrontcoverofthisbook.

Students are NOT permitted to bring mobile phones and/or any other unauthorised electronic devices into the examination room.

©VICTORIANCURRICULUMANDASSESSMENTAUTHORITY2018

SUPERVISOR TO ATTACH PROCESSING LABEL HEREVictorian Certificate of Education 2018

STUDENT NUMBER

Letter

2018VETLABSKILLSEXAM 2

SECTION A – continued

Question 1Whatinformationshouldbeincludedonthelabelforanagarstreakplatethathasbeenstreaked?A. employeename,dateofbirth,sampleidentificationB. employeename,clientname,dateofsamplereceiptC. dateofsamplereceipt,laboratoryname,roomtemperatureD. employeename,dateofsamplepreparation,sampleidentification

Question 2Whenusingacompoundmicroscopewithitshigh-powerobjectivelens,whichoneofthefollowingshouldnot beadjusted?A. coarsefocusB. diaphragmC. stageclipsD. finefocus

Question 3 Laboratorystaffhavebeeninformedbythelaboratorysupervisorthatanewmodelofsterilisingequipmentwillbeinstalledinthelaboratorythefollowingweek.Basedonthisinformation,whatactionshouldaninexperiencedtechniciantake?A. Waitforthelaboratorysupervisortotellthemwhenthetrainingforthenewequipmentmodelwilltake

place.B. Researchinformationaboutthenewequipmentmodelbeforeitarrives,tohelptraintheircolleagueson

howtouseit.C. Researchinformationaboutthenewequipmentmodel,thenaskthelaboratorysupervisorabouttraining

opportunities.D. Researchinformationaboutthenewequipmentmodelandinformthelaboratorysupervisorthattheydo

notrequireanytraining.

Question 4Thecorrectprocedureisbeingusedtoprepareprimarystandardsolutionsaccurately.Duringthisprocedure,quantitativetransferofasolidisusedinordertoA. transferanexactmassofsoluteduringpreparationofthesolution.B. operatethebalanceaccordingtothemanufacturer’sinstructions.C. findaprobableestimateofthefinalconcentrationofthesolution.D. ensurethecorrectmaterialsarechosenforpreparationofthesolution.

SECTION A – Multiple-choice questions

Instructions for Section AAnswerallquestionsinpencilontheanswersheetprovidedformultiple-choicequestions.Choosetheresponsethatiscorrectorthatbest answersthequestion.Acorrectanswerscores1;anincorrectanswerscores0.Markswillnotbedeductedforincorrectanswers.Nomarkswillbegivenifmorethanoneansweriscompletedforanyquestion.

3 2018VETLABSKILLSEXAM

SECTION A – continuedTURN OVER

Question 5A dilution of potassium chloride, KCl, is to be prepared in the laboratory. 100 mL of a 0.1 M solution of KCl is transferred to a 200 mL volumetric flask and deionised water is added to the mark. The deionised water is best identified as theA. liquid.B. solute.C. solvent.D. solution.

Question 6A laboratory technician receives a call from a client’s partner, requesting the client’s test results.What is the most appropriate action that should be taken by the laboratory technician? A. Retrieve the test results and then pass the details on to the client’s partner efficiently and politely. B. Request further identifying details about the client and their test, and inform the partner that the client’s

results are not yet ready to be released. C. Request further identifying details about the client and tell the partner that they will be informed of the

test results once the results are released. D. Explain that the test results cannot be released to anyone other than the client and request that the client

calls the laboratory for the results.

Question 7Duplicate sample test values were found to be out of the expected range.When this occurs, the sample test values should beA. retested every time. B. ignored as an odd occurrence.C. reported and noted in the workplace recording system. D. treated as a serious breakdown in quality control.

Question 8Samples from a potential new supplier of hazardous chemical reagents for a laboratoryA. should be tested and evaluated before use.B. do not need to be supplied with safety data sheets (SDS).C. should only be reordered if there are significant cost savings.D. should not be used, in order to avoid changes to important standard operating procedures (SOP).

Question 9What is the correct ergonomic posture for a laboratory technician working with a microscope in a seated position?A. straight back and neck, arms level with the bench, knees bent 45° and toes stretched to touch the floorB. straight back and neck, arms level with the bench, knees bent 45° and feet resting level on the floorC. straight back and neck, arms leaning forward onto the bench, knees bent 45° and feet resting level on

the floorD. straight back, arms leaning forward onto the bench, neck stretched towards the microscope eyepieces,

and legs relaxed and hanging from a high stool


SECTION A – continued

Question 10 Whenrotatingtheobjectivelensonamicroscopetoahigher-poweredlens,theA. diaphragmmustbeclosed.B. lightintensitymustbeincreased.C. lightintensitymustbedecreased.D. workingdistancemustbeincreased.

Question 11 Afoodsamplerequiringurgentbacterialcontaminationtestshasarrivedatalaboratory.ThemostappropriateactionforalaboratorytechniciantotakewouldbetoA. informthelaboratorysupervisoraboutthetestrequestandwaitforthesupervisortoassigntheworkto

alaboratorytechnician.B. testthefoodsampleimmediately,butonlyafterreferringtothetestrequestspecifications.C. testthefoodsampleimmediatelywithageneralgrowthmediatoseewhatgrows.D. checkwiththelaboratorysupervisorfirstbeforeanytestingisdone.

Question 12Thebestwaytosteriliseaninoculatingloop,whileminimisingtheformationofaerosols,istoA. soaktheloopinasterilisingsolutionfor24hours.B. placetheloopdirectlyintheblueflameofaBunsenburnerandholditthereforafullminute.C. firstheatthetipoftheloopgraduallyintheflameofaBunsenburner,beginningwiththehandleendof

thewire.D. placetheloopdirectlyintheorangeflameofaBunsenburnerandpassitbackandforthuntiltheloop

glowsred.

Question 13Alaboratorytechnicianpreparessomeagarstreakplatesfromabrothculture.Duringthenextshift,anotherlaboratorytechniciancheckstheagarstreakplatesafterincubationandobservesconfluentgrowthinallsectorsoftheplates.ThebestexplanationforthisobservationwouldbethatthepreviouslaboratorytechnicianA. didnotflametheneckofthebrothbottle.B. forgottoflametheloopbetweensectors.C. wasnotworkingneartheBunsenburnerflame.D. forgottocleanthebenchbeforestartingtheprocedureforpreparingtheagarstreakplates.

Question 14GlasswareandothersolidmaterialsthatrequireautoclavingA. donotrequirelabellingasliquidswould.B. mustonlybesterilisedwiththeautoclavesetonadrycycle.C. mustonlybesterilisedwiththeautoclavesetonawetcycle.D. canbesterilisedinamixedloadwiththeappropriateautoclavesettings.


SECTION A – continuedTURN OVER

Question 15Whichoneofthefollowingwouldcontributethemosttoreducingtheshelflifeofaworkingchemicalsolution?A. returninganydecantedsolutiontothebottleB. makingupasolutionimmediatelybeforeitisrequiredC. keepingthesolutioninarefrigeratorwhileitisnotbeingusedD. wrappingthesolutioninaluminiumfoilwhenitislight-sensitive

Question 16Alaboratorytechnicianwouldidentifythephysicalhazardsignforanoxidisingsubstancebywhichoneofthefollowingpictograms?

A. B. C. D.

Sources:StandardStudio/Shutterstock.com(A.,C.,D.); Migrenart/Shutterstock.com(B.)

Question 17Alaboratorytechnicianhasbeenaskedtocarryoutastarchtestonapotatosample.Whichofthefollowingpairsofstainandcolourstructureswouldshowapositiveresultwhenexaminedunderamicroscope?A. Gramstain,pinkgranulesB. iodinestain,browngranulesC. iodinestain,blue-blackgranulesD. Gramstain,purple-blackgranules

Question 18Thelaboratorysupervisorinanenvironmentaltestinglaboratoryinstructsatechniciantoasepticallytransfersomesoilsamplesformicrobialevaluation.TransferringasepticallyinthisinstancewouldbestbeachievedbyA. sterilisingeachsamplebyheatundersteampressure.B. storingculturesinaculturecollectionforfuturestudyorreference.C. applyingatreatmentthatresultsindeathorremovalofalllivingorganismsinthesamples.D. usingproceduresforhandlingcultures,mediaandequipmentthatensurenocontamination,andthat

onlythedesiredorganismsarepresent.


END OF SECTION A

Question 19ApathologylaboratoryreceivesanoticefromtheNationalAssociationofTestingAuthorities(NATA),Australia,aboutanauditvisit.TheinformationthattheauditorsarelikelytoconcentrateonaretheA. makesandmodelsofthelaboratoryequipment.B. laboratoryresources,proceduresanddocumentation.C. profitabilityandefficiencyofthetestingprocedures.D. speedandefficiencywithwhichthelaboratorytechnicianscompletetheirtasks.

Question 20Whatisthevolumeofa50×stocksolutionthatisrequiredtoprepare200mLofa1.0×workingsolution?A. 4.0mLB. 40.0mLC. 400mLD. 0.04L


SECTION B – continuedTURN OVER

Question 1 (6marks)a. Apathologylaboratorytechnicianreceivesacallfromaveterinarysurgeonrequestingan

urgentresultonasubmittedbloodsample.

Listthreepiecesofinformationthatthetechnicianwillneedtoobtainfromtheveterinarysurgeonwhilestillonthephone. 3marks

1.

2.

3.

b. Theveterinarysurgeonhasrequestedthatthesamplebetestedforbacteria.

Whatlaboratorytestwouldneedtobecarriedouttohelpidentifythepossiblestrainofbacteria? 1mark

c. Thetestcarriedoutinpart b.isexpectedtoprovideoneoftwopossibleresults.

Namethetwopossibleresultsandgiveonecharacteristicthatwouldhelptoidentifyeachresult. 2marks

Result1

Characteristic

Result2

Characteristic

SECTION B – Short-answer questions

Instructions for Section BAnswerall questionsinthespacesprovided.


SECTION B – continued

Question 2 (3marks)Alaboratorytechniciansuspectsthattheelectronicbalancebeingusedinthelaboratoryisinaccurate.

a. Suggestoneactionthatthetechniciancouldtaketosupportthelaboratory’s‘rightfirsttime’objective. 1mark

b. Identifytwoincidentsofwastagethattheinaccurateelectronicbalancecouldcreatewithinthecompany,ifthetechniciandidnotactpromptlytoaddressthesituation. 2marks

1.

2.

Question 3 (3marks)Fromthefollowinglist,identifythemostappropriatecleaningordisposalmethodforeachofthelaboratoryitemsgiveninthetablebelow.

biohazardwastebin sharpscontainer 1.0%hypochloritesolution

Laboratory item Appropriate cleaning or disposal method

glasscell-countingchamberorhaemocytometer

plasticflaskofcellculturemedia

usedscalpelbladesfrombiologicaldissection



Question 4 (5marks)Primarystandardsolutionsareusedextensivelyinanalyticalchemistrylaboratories.

a. Giveonereasonforusingprimarystandardsolutionsinlaboratorywork. 1mark

b. Whattwostepsshouldatechniciantaketomakethemselvesfamiliarwithalaboratory’sprotocolforpreparingprimarystandardsolutions? 2marks

1.

2.

c. Thequantitativetransferofsolidsisarequirementwhenpreparingprimarystandardsolutions.

Explainwhatismeantby‘quantitativetransferofsolids’andgiveonereasonforitsimportance. 2marks



Question 5 (7marks)AlaboratorytechnicianhasbeenaskedtopreparebacterialsmearsandtostainthemusingtheGramstainmethodformicroscopicexamination.

a. Beforebeginningobservationoftheslides,whataretwochecksthatshouldbecarriedouttoensurethatthemicroscopeisfitforpurpose? 2marks

1.

2.

b. Identifytheobjectivelensthatshouldbeusedtoobservethebacterialsmearsonthepreparedslides. 1mark

c. Indicatewhichofthetwodiagramsofslidesbelowshowsthecorrectmicroscopeset-upformagnificationandforresolutionoftheimageonapreparedGramstainslide.Tick()theboxforyourchosenslide,AorB.

DiagramofSlideA

microscope lens

immersion oil

microscopeslide

DiagramofSlideB

microscope lens

immersion oil

microscopeslide

GivetworeasonsforyourchoiceofSlideAorSlideB. 2marks

1.

2.

d. Describetwoadvantagesofusingimmersionoilonaslide. 2marks



Question 6 (5marks)a. IdentifytwolaboratoryworktasksthatwouldbeincludedinaNationalAssociationofTesting

Authorities(NATA),Australia,auditonquality. 2marks

1.

2.

b. Givethreereasonswhyqualityauditsshouldbecarriedoutregularly. 3marks

1.

2.

3.

Question 7 (4marks)Thegeneraldutiesofalaboratorytechnicianincludethepreparationandpouringofnutrientagarstocksforgenerallaboratoryuse.

Whataretwoquality-controlstepsalaboratorytechniciancouldtaketoensurethatnutrientagarstocksaresterile?Giveyourreasons.

Step1

Reason

Step2

Reason


SECTION B – Question 9–continued

Question 8 (6marks)Workinginthemicrobiologysectionofalaboratorytoprepareagarstreakplatesisoneoftheresponsibilitiesofalaboratorytechnician.

a. Givetwowaystoidentifycontaminationonagarplatesthathavebeenstreakedforsinglecolonieswithpureculturesofbacteria. 2marks

1.

2.

b. Otherthantheuseofpersonalprotectiveequipment(PPE),givefourapproachesusedtomaintainhygienewhenworkinginthemicrobiologysectionofalaboratory. 4marks

1.

2.

3.

4.

Question 9 (5marks)Alaboratorytechnicianhasbeenaskedtoprepare250mLofaproteinsolutionof2.0%w/vbovineserumalbumin(BSA).ThetechniciannoticesthattheBSAproteinisaveryfinepowderandeasilybecomesairborne.

a. Givetwoactionsthatthetechnicianmusttakeinthissituationtoavoidpossiblesafetyhazards. 2marks

1.

2.

b. Thestandardoperatingprocedure(SOP)toprepareaBSAproteinsolutionspecifiesthatitsfinalpHmustbe7.6

IdentifyoneprecautionthatshouldbespecifiedwithintheSOPfortheprocessofdissolvingandadjustingthepHofthesolution. 1mark



c. BSAisabiologicalsubstanceisolatedfrombeefblood.Thisbloodmaynothavebeensourcedlocallybutfromcountriesthatmayhavehadoutbreaksofnotifiablediseases.

IdentifyoneadditionalhealthriskthatthissubstancemaypresentandsuggestwhattoavoidwhenhandlingBSA. 2marks

Healthrisk

Whattoavoid

Question 10 (3marks)IdentifyonehazardofworkingwithliquidnitrogenordryiceinalaboratoryandoneitemofPPEthatwouldneedtobeworn.Describetheriskthatmaybepresentintheenvironmentwhenworkingwiththesesubstances.

Hazard

ItemofPPE

Risk

Question 11 (2marks)Thebiosafetycabinetinalaboratoryhasbeendecontaminatedbycontractors.Thedecontaminationprocedureinvolvesusinghazardousformaldehydevapourandisolatingthelaboratorywherethecabinetislocatedforseveraldays.However,nonoticeofthisprocedurewaspostedattheentrancetothelaboratoryinthisinstance.Atechnicianhasbeenassignedtoperformaroutinecellcultureprocedureinthelaboratorythefollowingdayandnoticesthatthecabinethasbeensealedwithtape.

Suggesttwoapproachesthetechniciancouldtaketodealwiththissituation.

1.

2.



Question 12 (5marks)a. Figure1belowshowsabloodsmearfromasample.

Circlethepartofthebloodsmearthatwouldidentifyseparatedbloodcellswhenobservingtheslidewithamicroscope. 1mark

slide

smear

Figure 1

b. OnFigure2below,drawalinetoidentifyandlabeleachofthefollowing:• awhitebloodcell• aredbloodcell• aplatelet 3marks

Figure 2

c. Suggestonemethodofcheckingthatablood-smear-stainedslidewillmeetthequality-controlstandardsrequiredbyalaboratory. 1mark



Question 13 (7marks)Atechnicianhasbeenaskedtorestockalaboratory’ssolutions.Thistaskinvolvesmakingupsomenewsolutionsofcalciumcarbonate,CaCO3,andpotassiumchloride,KCl.

a. Completethetablebelowbycalculatingthemassrequiredtomakeeachsolution.Includetheunitofmeasurementandshowyourworking. 4marks

Data

formulaweightofCaCO3 100g/mol

formulaweightofKCl 74g/mol

CalculationsformassofCaCO3

CalculationsformassofKCl

Chemical Mass Molarity Volume

CaCO3 0.4M 350mL

KCl 0.5M 100mL

b. WhatisthemostappropriatepieceofequipmentthatshouldbeusedtostoretheCaCO3andKClsolutions? 1mark

c. Thetechnicianalsomakesupa70%v/vethanolsolution.Thelabelledcontainerhasthefollowinginformation:70%v/vethanol.

Listtwootherpiecesofinformationthatwillneedtobeincludedonthislabel. 2marks

1.

2.



Question 14 (6marks)Atechnicianusinga200–1000µLvariablepipettesuspectsthatthepipetteisnotalwaysdeliveringthecorrectvolume.Thetechniciandecidestoperformacalibrationprocedure.

a. Givetworeasonswhycalibrationproceduresareimportantinlaboratories. 2marks

1.

2.

b. Asthevariablepipetteismostlyusedfordelivering1000µLvolumes,thetechnicianperformsthecalibrationatthissetting,notingthattheroomtemperatureis20°Candmeasuringtheindividualmassesofdistilledwaterdeliveredbythepipettefor20deliveries.Thetechnicianthenconvertsthemassestovolumesusingtheformula

volume = massdensity

Ifthedensityofpurewaterisassumedtobe1.0000g/mLat20°C,calculatetheactualvolumedeliveredbythepipetteforadeliveredmassof0.900g. 2marks

c. Thetechniciannotesthatmostofthemassesdeliveredbythevariablepipetteareclosetothevalueof0.900gandfallwithintherangeof0.898gto0.906g.

Whatdeliveredmasswouldthetechnicianexpectifthepipettewasoperatingcorrectlyatasettingof1000µL? 2marks



Question 15 (3marks)Alaboratorytechnicianhasbeenaskedtocontributetoupdatingthelaboratory’sSOPforinoculatingsterilenutrientbroth(NB)fromaculturegrowingonanagarplate.Severalstepsarerequired.Thefirstfourstepsaregiveninthetablebelow.

Completethetablebyenteringtheremainingthreestepsoftheprocedure.

Step number Procedure

1 Disinfectthebenchwith70%v/vethanolorsimilar.

2 LighttheBunsenburner.

3 Gathermaterialsandensureallmaterialsarewithinreach.

4 Sterilisetheinoculatingloop.

5

6

7



Question 16 (4marks)Alaboratorytechnicianispreparing250mLofa0.10Msolutionofcoppersulphate,CuSO4,using achemicalbottlewithlabelsasshownbelow.

CAUTION

Keep out of reach of children.

ACME Laboratories

Copper(II) sulphate.5H2O

Formula weight: 249.68

MIN

Assay 98.0%

Front label Side label

a. CalculatethemassofCuSO4thatwouldberequiredtoaccuratelyprepare250mLofa0.10MsolutionofCuSO4.Showyourworking. 2marks

b. ThetechnicianisrequiredtouseabottleofanhydrousCuSO4toprepareasecond250mLsolutionof0.10MCuSO4.

WouldthemassofCuSO4calculatedinpart a.stillbeaccurate?Giveyourreasoning. 2marks


Question 17 (6marks)Apathologylaboratoryisinvestigatingwaystoimproveitsproceduresandoutput.Oneoftheareasbeinginvestigatedistemperatureregulationinthelaboratory’sincubator.Thetemperatureoftheincubatorismonitoredandrecordedthroughoutanormalworkingday.Theresultsareshowninthegraphbelow.

8.00 am 9.00 am 10.00 am 11.00 am 12.00 noontime of day

1.00 pm 2.00 pm 3.00 pm 4.00 pm 5.00 pm

10

15

20

25temperature(°C)

30

35

40

a. Basedonthegraph,whatoperatingtemperaturehasthethermostatintheincubatorbeensettoinordertooptimiseculturegrowthatthebeginningofeachday?Expressyouranswertoonedecimalplace. 1mark

b. Thegrowthrateofculturesisnotaffectedbyatemperaturechangeof±2°C.

Usingtheinformationinthegraph,givethetwotimeperiodswhenthetemperaturedroppedbelowthisoptimumtemperaturerange. 2marks

c. Suggestonereasonforthetemperaturedropatthetwotimeperiodsgiveninpart b. 1mark

d. Usingtheinformationinthegraph,giveonereason,otherthanthetemperaturebeingoutsideofthenormalrange,foranobservedreductioninthegrowthrateoftheculturesandsuggestapossiblesolution. 2marks

END OF QUESTION AND ANSWER BOOK

vce vet laboratory skills · 3 2018 vet lab skills exam section a continued turn over question 5 a...

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