Curriculum Vitae

V. Anne Westbrook, Ph.D. 500 Bradley Ct., DFrederick, MD 21703240-440-2878westbrookva@gmail.com

EDUCATION

Ph.D. July 1994, Vanderbilt University Medical Center, Nashville, TNAdviser - Gary E. Olson, Ph.D.Department of Cell Biology

B.S. May 1988, Louisiana State University, Baton Rouge, LAMajor in Zoology

RESEARCH EXPERIENCE

Research Associate Department of Biochemistry and Molecular Genetics, Cell Migration Consortium, University of Virginia Health Science Center, Charlottesville, VA, Supervisor, William R. Pearson, April 2003-present

RefSeq Scientist Computercraft Corporation, National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD, Supervisor, Jennifer Hart, Ph.D. June 2002-February 2003

Research Scientist Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA, Supervisor, John C. Herr, Ph.D. 1999-June 2002

Post-doctoral Fellow Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA, Adviser, John C. Herr, Ph.D. 1994-1998

Doctoral Thesis Structural Organization and Biochemical Characterization of the Domain-Restricted Proteins PM35/52 and AM50 in Spermatids and Spermatozoa of the Guinea Pig. Department of Cell Biology, Vanderbilt University Medical Center, Nashville, TN, Adviser, Gary E. Olson, Ph.D. 1988-1994

NSF Summer Research Internship Department of Zoology and Physiology, Louisiana State University, Baton Rouge, LA Advisers: John W. Lynn, Ph.D. and Earl H. Weidner, Ph.D. 1987

Undergraduate Research Department of Zoology and Physiology, Louisiana State University, Baton Rouge, LA Adviser: John W. Lynn, Ph.D. 1985-1988

TEACHING EXPERIENCES

Volunteer Tutor Literacy Council of Frederick County: Assisted both American and foreign-born adults in attaining basic reading, writing and English-speaking skills with one-on-one tutoring – resulting in lives of greater productivity and communities with a higher quality of life. 2011-2012.

Supervision of Student Research University of Virginia: Supervised medical, graduate, and undergraduate student research projects during academic year and summer programs. Served as a mentor in summer research programs for minority students. June 1994 - 2002

Instructor Marine Biological Laboratory, Woods Hole, MA: Frontiers in Reproduction. Co-taught workshop on sperm antigens. June 1998, June 1999

Colloquium Organizer University of Virginia: Co-taught graduate student colloquium. Topic: Gene regulation during spermatogenesis. November 1997 - April 1998

Teaching Assistant Vanderbilt University Medical School: Histology, Introduction to Cell Biology, and Reproductive Biology. Lectured, conducted review sessions, prepared and graded exams. January 1989 – January 1994

Teaching Assistant Louisiana State University: Laboratory courses in Introductory Biology, Zoology, and Developmental Biology. September 1985 - May 1988

PUBLICATIONS

JOURNAL ARTICLES

Kouprina N., Noskov V.N., Pavlicek A., Risinger J.I., Kim J.H., Westbrook V.A., Solomon G., Sounders H., Herr J.C., Jurka J., Lobanenkov V., Schlessinger D., Collins N.K., Schoppee Bortz P.D., Ottolenghi C., Loukinov D., Goldsmith P., Larionov V. (2007) Evolutionary Diversification of SPANX-N Sperm Protein Gene Structure and Expression..PLoS ONE. Apr 4;2:e359.

Westbrook, V.A., Schoppee, P.D., Vanage, G.R., Klotz, K.L., Diekman, A.B., Flickinger, C.J., Coppola, M.A., Herr J.C. (2006) Hominoid-specific SPAN-XA/D genes demonstrate differential expression in individuals and protein localization to a distinct nuclear envelope domain during spermatid morphogenesis. Mol Hum Reprod 12:703-16.

Hao, Z., Jha, K.N., Kim, Y.H., Vemuganti, S., Westbrook, V.A., Chertihin, O., Markgraf, K., Flickinger, C.J., Coppola, M., Herr, J.C., Visconti, P.E. (2004) Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm. Mol Hum Reprod 10:433-44.

Westbrook, V.A., Schoppee, P.D., Diekman, A.B., Klotz, K.L., Allietta, M., Hogan, K.T., Slingluff, C.L., Patterson, J.W., Frierson, H.F., Irvin, W.P. Jr, Flickinger, C.J., Coppola, M.A., Herr, J.C. (2004) Genomic organization, incidence, and localization of the SPAN-x family of cancer-testis antigens in melanoma tumors and cell lines. Clin Cancer Res 10:101-12.

Shetty, J., Wolkowicz, M.J., Digilio, L.C., Klotz, K.L., Jayes, F.L., Diekman, A.B., Westbrook, V.A., Farris, E.M., Hao, Z., Coonrod, S.A., Flickinger, C,J,, Herr, J,C. (2003) SAMP14, a novel, acrosome membrane associated, GPI anchored member of the Ly-6/uPAR receptor superfamily with a role in sperm-egg interaction. J Biol Chem 278:30506-15.

Wolkowicz, M.J., Shetty, J., Westbrook, A., Klotz, K., Jayes, F., Mandal, A., Flickinger, C.F., Herr, J.C. (2003) Equatorial segment protein defines a discrete acrosomal subcompartment persisting throughout acrosomal biogenesis. Biol Reprod 69:735-45.

Ficarro, S., Chertihin, O., Westbrook, A., White, F., Jayes, F., Kalab, P., Shabanowitz, J., Herr, J.C., Hunt, D., Visconti, P.E. (2003) Phosphoproteome analysis of human sperm. Evidence of tyrosine phosphorylation of AKAP 3 and valosin containing protein/P97 during capacitation. J Biol Chem 278:11579-89.

Norton, E.J., Diekman, A.B., Westbrook, V.A., Klotz, K.L., Thomas, T.S., Wright, D.C., Brisker, J., Flickinger, C.J., Herr, J.C. (2002) A male genital tract-specific carbohydrate epitope on human CD52: Implications for immunocontraception. Tissue Antigens 5:354-64.

Hao Z., Stoler M.H., Sen B., Shore A., Westbrook A., Flickinger C.J., Herr J.C., Coonrod S.A. (2002) TACC3 statement and localization in murine egg and ovary. Mol Reprod Dev 63:291-9.

McCauley, T.C., Kurth, B.E., Norton, E.J., Klotz, K.L., Westbrook, V.A., Rao, A.J., Herr, J.C., Diekman, A.B. (2001) Analysis of a human sperm CD52 glycoform in primates: Identification of an animal model for immunocontraceptive vaccine development. Biol Reprod. 66:1681-8.

Hao, Z., Wolkowicz, M.J., Shetty, J., Klotz, K.L., Bolling, L., Sen, B., Westbrook, V.A., Coonrod, S.A., Flickinger, C.J., Herr, J.C. (200) SAMP32, a testis-specific, acrosomal membrane-associated isoantigen, has a role in sperm-egg binding-fusion. Biol Reprod. 66:735-44.

Naaby-Hansen S., Mandal A., Wolkowicz M.J., Sen B., Westbrook V.A., Shetty J., Coonrod S.A., Klotz K.L., Kim Y.H., Bush L.A., Flickinger C.J., Herr J.C. (2002) CABYR, a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation. Dev Biol. 242:236-54.

Visconti, P.E., Westbrook, V.A., Chertihin, O., Demarco, I., Sleight, S., Diekman, A.B. (2001) Novel signaling pathways involved in sperm acquisition of fertilizing capacity. J Reprod Immunol 53:133-150.

Naaby-Hansen, S., Wolkowicz, M.J., Klotz, K., Bush, L., Westbrook, V.A., Shibahara, H., Shetty, J., Coonrod, S.A., Reddi, P.P., Shannon, J., Kinter, M., Sherman, N.E., Fox, J., Flickinger, C.J., Herr, J.C. (2001) Co-localization of the inositol 1,4,5-triphosphate receptor and calreticulin in the equatorial segment and in membrane bounded vesicles in the cytoplasmic droplet of human spermatozoa. Mol Hum Reprod 7:923-933.

Norton, E.J., Diekman, A.B., Westbrook, V.A., Flickinger, C.J., Herr, J.C. (2001) RASA, an anti-sperm ScFv directed against the sperm glycoform of CD52: Implications for novel contraceptives. Hum Reprod 16:1854-1860.

Westbrook, V.A., Diekman, A.B., Naaby-Hansen, S., Coonrod, S.L., Klotz, K.L., Thomas, T.S., Norton, E.J., Flickinger, C.J., Herr, J.C. (2001) Differential nuclear localization of the cancer-testis associated protein SPAN-X/CTp11 in transfected cells and in 50% of human spermatozoa. Biol Reprod 64:345-358.

Westbrook, V.A., Diekman, A.B., Klotz, K.L., Khole, V.V., Von Kap-Herr, C.,Golden, W.L., Eddy, R.L., Shows, T.B., Stoler, M.H., Lee, C.G., Flickinger, C.J., and Herr, J.C. (2000) Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa. Biol Reprod 63:469-481.

Diekman, A.B., Norton, E.J., Klotz, K.L., Westbrook, V.A., Shibahara, H., Naaby-Hansen, S., Flickinger, C.J., Herr, J.C. (1999) N-linked Glycan of a sperm CD52 glycoform associated with human infertility. The FASEB Journal 13:1303-1313.

Diekman, A.B., Norton, E.J., Klotz, K.L., Westbrook, V.A., Herr, J.C. (1999) Evidence for a unique N-linked glycan associated with human infertility on sperm CD52: A candidate contraceptive vaccinogen. Immunol Reviews 171:203-211.

Diekman, A.B., Norton E.J., Westbrook, V.A., Klotz, K.L., Naaby-Hansen, S., Herr, J.C. (2000) Anti-sperm antibodies from infertile patients and their cognate sperm antigens: A review. Identity between SAGA-1, the H6-3C4 antigen, and CD52. American J Reprod Immunol 43:134-143.

Olson, G.E., Winfrey, V.P., Westbrook, V.A., Melner, M.H. (1998) Targeting of the domain-specific integral membrane protein PM52 to the periacrosomal plasma membrane during guinea pig spermiogenesis. Mol Reprod Devel 50: 103-112.

Diekman, A.B., Westbrook-Case, V.A., Klotz, K.L., Naaby-Hansen, S., Flickinger, C.J., Herr, J.C. (1997) Biochemical characterization of Sperm Agglutination Antigen-1, a human sperm surface antigen implicated in gamete interaction. Biol Reprod 57: 1136-1144.

Westbrook-Case, V.A., Winfrey, V.P., Olson, G.E. (1995) Sorting of the domain-specific acrosomal matrix protein AM50 during spermiogenesis in the guinea pig. Devel Biol 167: 338-349.

Westbrook-Case, V.A., Winfrey, V.P., Olson, G.E. (1994) Characterization of two antigenically related integral membrane proteins of the guinea pig sperm periacrosomal plasma membrane. Mol Reprod Devel 39: 309-321.

Westbrook-Case, V.A., Winfrey, V.P. Olson, G.E. (1994) A domain-specific 50 Kilodalton structural protein of the acrosomal matrix is processed and released during the acrosome reaction in the guinea pig. Biol Reprod 51: 1-13.

BOOK CHAPTER

Westbrook, V.A., Diekman, A.B., Visconti, P.E. (2002) Novel signaling pathways involved in sperm acquisition of fertilizing capacity. In: Introduction to Mammalian Reproduction. (Ed. D.R. Tulsiani). Kluwer Academic/Plenum Publishers, New York, NY.

PATENTS

Herr, J.C., Diekman, A.B., Norton, E.J., Westbrook-Case, V.A. (2001) Sperm surface antigen, monoclonal antibody therefor and applications therefor. U.S. Patent #6,258,346, issued July 10, 2001.

Herr, J.C., Diekman, A.B., Norton, E.J., Westbrook-Case, V.A. (1998) Sperm surface antigen, monoclonal antibody therefor and applications therefor. U.S. Patent #5,830,472, issued November 3, 1998.

Westbrook, V.A., Herr, J.C., Diekman, A.B. (1998) X-linked, Spermatid-Specific expression of a nuclear associated protein (NAP-X) present in 50% of human spermatozoa. Provisional U.S. Patent Application #60/105,324, filed October 23, 1998. Converted October 22, 1999.

Westbrook, V.A., Herr, J.C., Diekman, A.B. (1999) Nucleic acids and proteins of the human SPAN-X gene and uses thereof. U.S. Patent Application # US99/24973, filed October 22, 1999. Abandoned April 12, 2001.

Westbrook, V.A., Herr, J.C., Diekman, A.B., Coppola, M.A. (2000) Use of SPAN-X for identification, diagnosis, and treatment of cancer. Provisional U.S. Patent Application #60/218,904, filed July 18, 2000. Expired.

Herr, J.C., Westbrook, V.A., Schoppee, P.D., Klotz, K.L. (2003) Use of SPAN-X for identification, diagnosis, and treatment of cancer. Provisional U.S. Patent Application, #60/496,497, filed August 20, 2003.

PUBLISHED ABSTRACTS

Hao, Z., Wolkowicz, M.J., Shetty, J., Klotz, K., Coonrod, S.A., Bolling, L., Sen, B., Westbrook, V.A., Flickinger, C.J., Herr, J.C. (2001) SAMP32, a novel, testis-specific, membrane-associated protein, is localized to the acrosome of human spermatozoa. Biol Reprod 64 (Supplement): 224a.

Westbrook, V.A., Coppola, M.A., Diekman, A.B., Herr, J.C. (2001) Expression of the cancer-testis antigen SPAN-X in multiple tumor types. Am J Reprod Immunol 46:109.

Khole, V.V., Westbrook, V.A., Mandel, A., Herr, J.C., Visconti, P. (2001) Cloning and characterization of human testicular soluble adenylyl cyclase. Am J Reprod Immunol 46:63.

Diekman, A.B., Norton, E.J., McCauley, T.C., Westbrook, V.A., Anderson, P., Kurth, B.E., Klotz, K.L., Easton, R., Dell, A., Morris, H., Herr, J.C. (2001) Localization of a sperm CD52 carbohydrate epitope during epididymal maturation, capacitation, and the acrosome reaction. J Androl May/June (Supplement):202.

Westbrook, V.A., Diekman, A.B., Coonrod, S.A., Klotz, K.L., Buer, S., Herr, J.C. (2000) Characterization of SPAN-X in the mammalian testis and transfected cells. Biol Reprod 62 (Supplement 1):582a.

McCauley, T.C., Diekman, A.B., Norton, E.J., Kurth, B.E., Westbrook, V.A., Herr, J.C. (2000) Analysis of sperm agglutination antigen-1 (SAGA-1), a glycoprotein implication in human infertility. Biol Reprod 62 (Supplement 1):124a.

Hao, Z., Coonrod, S., Shore, A., Buer, S., Westbrook, A., Flickinger, C.J., Herr, J.C. Cloning of TACC3 from mouse oocyte proteome. Frontiers in Biology of Reproduction, Boston, MA, June 2000.

Norton, E.J., Diekman, A.B., Westbrook, V.A. Klotz, K.L., Herr, J.C. (1999) RASA: A recombinant anti-sperm antibody directed against the sperm glycoform of CD52. Molecular Biology of the Cell 10(Supplement):359a.

Norton, E.J., Diekman, A.B. Westbrook, V.A., Klotz, K.L., Herr, J.C. (1998) Immunohistochemical localization of a carbohydrate epitope in the human reproductive tract: Implications for spermicidal development. The FASEB Journal 12:A1104.

Westbrook, V.A., Diekman, A.B., Klotz, K.L., Naaby-Hansen, S., Lai, W., Herr, J.C. (1998) Characterization and localization of the human sperm protein NAP-X. Molecular Biology of the Cell 9(Supplement):178a.

Diekman, A.B., Klotz, K.L.,Westbrook-Case, V.A., Norton, E.J., Naaby-Hansen, S., Herr, J.C. (1997) Biochemical characterization and purification of sperm agglutination antigen-1; A human sperm surface antigen implicated in gamete interactions. Journal of Reproduction Immunology 34:29-30.

Westbrook-Case, V.A., Diekman, A.B., Khole, V.V., Herr, J.C. (1995) Characterization of the acrosomal matrix protein P71/P72 in human spermatozoa. Molecular Biology of the Cell Supplement:428a.

Westbrook-Case, V.A., Winfrey, V.P., Olson, G.E. (1994) Sorting of the domain-specific acrosomal matrix protein AM50 occurs during guinea pig spermiogenesis. Biology of Reproduction.

PRESENTATIONS

Westbrook, V.A., Coppola, M.A., Diekman, A.B., Herr, J.C. Expression of the cancer-testis antigen SPAN-X in multiple tumor types. Poster presentation at VIII International Congress of Reproductive Immunology, Opatija, Croatia, July 2001. Outstanding Poster Award.

Khole, V.V., Westbrook, V.A., Mandel, A., Herr, J.C., Visconti, P. (2001) Cloning and characterization of human testicular soluble adenylyl cyclase. Selected oral presentation at VIII International Congress of Reproductive Immunology, Opatija, Croatia, July 2001. Presenter, V.A. Westbrook.

Diekman, A.B., Norton, E.J., McCauley, T.C., Westbrook, V.A., Anderson, P., Kurth, B.E., Klotz, K.L., Easton R., Dell, A., Morris, H., Herr, J. C. Localization of a sperm CD52 carbohydrate epitope during epididymal maturation, capacitation, and the acrosome reaction. Poster presentation during the President's Poster Session at the VII International Congress of Immunology, Montreal, Canada, June 2001.

Diekman, A.B., Norton, E .J., McCauley, T.C., Westbrook, V.A., Anderson, P., Kurth, B.E., Klotz, K.L., Easton R., Dell, A., Morris, H., Herr, J.C. The use of carbohydrate epitopes in immunocontraceptive development. Poster presentation at International Congress of Reproductive Immunology, Amsterdam, The Netherlands, November 2000.

Diekman, A.B., Norton, E .J., Klotz, K.L., Westbrook, V.A., Herr, J.C. The use of carbohydrate epitopes in immunocontraceptive development. Poster presented at the 6th European Training Course on Carbohydrates, Debrecen, Hungary, July 2000.

Norton, E.J., Diekman, A.B., Westbrook, V.A., Klotz, K.L., Herr, J.C. RASA: A recombinant anti-sperm antibody directed against the sperm glycoform of CD52. Poster presentation, American Society for Cell Biology annual meeting, Washington, D.C., December 1999.

Diekman, A.B., Norton, E .J., McCauley, T.C., Klotz, K.L., Westbrook-Case, V.A., Shibahara, H., Naaby-Hansen, S., Flickinger, C.J., Herr, J.C. N-linked glycan of a sperm CD52 glycoform associated with human infertility. Poster presentation, Testis Workshop, Louisville, KY, April 1999.

Westbrook, V.A., Diekman, A.B., Klotz, K.L., Naaby-Hansen, S., Lai, W., Herr, J.C. Characterization and localization of the human sperm protein NAP-X. Poster presentation, American Society for Cell Biology annual meeting, San Francisco, December 1998.

Diekman, A.B., Norton, E.J., Klotz, K.L., Westbrook-Case, V.A., Naaby-Hansen, S., Herr, J.C. Characterization of the S19 monoclonal antibody and its cognate antigen Sperm Agglutination Antigen-1: Application for development of a contraceptive vaccine and an antibody-based spermicide. Oral presentation, NIH Joint Contraceptive Centers annual meeting, Davis, CA, November 1997.

Diekman, A.B., Klotz, K.L., Westbrook-Case, V.A., Norton, E.J., Naaby-Hansen, S., Herr, J.C. Biochemical characterization and purification of Sperm Agglutination Antigen-1: A human sperm surface antigen implicated in gamete interactions. Oral and poster presentations, First International Congress on Experimental and Clinical Reproductive Immunobiology, Charlottesville, VA, October 1997.

Westbrook-Case, V.A., Khole, V.V., Diekman, A.B., McCanna, M.E., Stoler, M.H., Eddy, R.L., Shows, T.B., Golden, W.L., von Kap-Herr, C., Herr, J.C. Post-meiotic expression of an X-linked gene encoding a testis-specific human acrosomal matrix protein (hAM20). Poster Presentation, Gordon Conference, Reproductive Tract Biology, Plymouth, N.H., July 1996.

Diekman, A.B., Westbrook-Case, V.A., Herr, J.C. Identification and characterization of sperm agglutination antigen-1 (SAGA-1), a glycoprotein localized over the entire human sperm plasmalemma. Poster Presentation, Gordon Conference, Reproductive Tract Biology, Plymouth, N.H., July 1996.

Westbrook-Case, V.A., Diekman, A.B., Herr, J.C. Post-meiotic expression of an X-linked gene encoding a testis-specific protein, P71/72. Oral presentation, Mid-Atlantic Reproductive Biology meeting, Baltimore, MD, June 1996.

Westbrook-Case, V.A., Diekman, A.B., Khole, V.V., Herr, J.C. Post-meiotic expression of an X-linked gene encoding a testis-specific protein, P71/72. Oral presentation, NIH Joint Contraceptive Centers annual meeting, Bethesda, MD, March 1996.

Diekman, A.B., Westbrook-Case, V.A., Herr, J.C. Identification and characterization of SAGA-1, a glycoprotein localized over the entire human sperm plasmalemma. Oral presentation, NIH Joint Contraceptive Centers annual meeting, Bethesda, MD, March 1996.

Westbrook-Case, V.A., Diekman, A.B., Khole, V.V., Herr, J.C. Characterization of the acrosomal matrix protein P71/72 in human spermatozoa. Poster Presentation, The American Society of Cell Biology annual meeting, Washington, D.C., December 1995.

Diekman, A.B., Westbrook-Case, V.A., Herr, J.C. Identification and characterization of sperm Agglutination Antigen-1 (SAGA-1). Oral presentation, NIH Joint Contraceptive Centers annual meeting, New York, NY, February 1995.

Westbrook-Case, V.A., Winfrey, V.P., Olson, G.E. Sorting of the domain-specific acrosomal matrix protein AM50 occurs during guinea pig spermiogenesis. Poster presentation, Society for the Study of Reproduction annual meeting, Anne Arbor, Michigan, July 1994.

HONORS

Outstanding poster presentation, VIII International Congress of Reproductive Immunology, Opatija, Croatia, July 2001

Andrew W. Mellon Fellow, University of Virginia, 1997 – June 2002

NIH Postdoctoral Trainee in Cell And Molecular Biology of the Urogenital System, University of Virginia, 1994-1997

NIH Predoctoral Trainee in Reproductive Biology, Vanderbilt University, 1990 - 1993

NSF Research Internship, Louisiana State University, June-August 1987

Phi Beta Kappa, Louisiana State University, February 1988