vandana kumari_cv
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Curriculum Vitae
Vandana Kumari
Personal Statement
To show my ability, creativity, dedication and enthusiasm for work and to excel in the field of Biomedical and other related field by being a part of a research-oriented team with a challenging environment
Education Doctoral Studies (Ph.D) Humboldt University, Berlin, Germany (Dept. of Dermatology, Venereology & Allergology, Charite) Mar. 2010 – Apr. 2015 Immunology, Grade: Magna Cum laude (Very good)
Title: "Mechanisms underlying the regulatory function of tumor necrosis factor-α in skin inflammation“
Master of Science (M.Sc) VIT University, Vellore, India Jun. 2005 – Jun. 2007 Biomedical Genetics. Grade: 7.82 Title: “Identification of Gyrase associated proteins in Mycobacterium smegmatis”
Bachelor of Science (B.Sc) Bangalore University, Bangalore, India Apr. 2002 – Apr. 2005 Microbiology. Percentage: 63.29 Experience
Project Assistant Prof. V. Nagaraja, MCBL, Indian Institute of Science (IISc), Bangalore, India Jul. 2007 – Sep. 2009 Microbiology, Molecular biology, Cell biology
Title (1) : Purification and identification of interacting surface of C protein on E.coli RNA polymerase β’ subunit. (2) : Mechanism of transactivation of C at late promoter Plys, PI, PP and Pmom of bacteriopahge Mu.
Masters Dissertation Indian Institute of Science (IISc), Bangalore, India Nov. 2006- Apr.2007 Molecular biology
Title : “Identification of Gyrase associated proteins in Mycobacterium smegmatis”
Summer Training Indian Institute of Science (IISc), Bangalore, India Apr. 2006 - May 2006 Bioinformatics
Title : Isoelectric point (pI) distribution of different proteins and protein complexes from yeast Summer Training Banaras Hindu University (B.H.U), Varanasi India Apr. 2005 - May 2005 Microbial genetics
Title : Importance of Microbial Techniques in Biotechnology Publication
Swapna, G., Chakraborty, A., Kumari, V., Sen, R. and Nagaraja, V. (2011), Mutations in β′ subunit of Escherichia coli RNA polymerase perturb the activator polymerase functional interaction required for promoter clearance. Molecular Microbiology, 80: 1169–1185
19/1, 3rd
floor, Sia Prakasam towers, Sai colony, Belathur, Kadugudi, Bangalore, Karnataka- 560067, India
Mobile : +919035430074 Email : [email protected] Date of Birth : 14th August 1983
V. Kumari, M. Babina, T. Hazzan, M. Worm (2014), TSLP induction by skin irritation is independent of TNF-α,
but supported by IL-1. British Journal of Dermatology , doi: 10.1111/bjd.13465
Ganduri Swapna, Vandana Kumari, Valakunja Nagaraja, Different modes of transactivation of bacteriophage Mu late promoters by transcription factor C, PLOS ONE (in press)
V. Kumari, Kristina. H, Anja. K, M. Worm, Influence of a systemic treatment with alitretinoin on the skin barrier of chronic hand eczema patients. (Under preparation)
Poster and Award Ganduri Swapna, Atanau Chakraborty, Vandana Kumari, Ranjan Sen and Valakunja Nagaraja, Poster titled ‘Mutations in the β' subunit of E.coli RNA polymerase perturb the functional interaction required for C mediated transactivation in phage Mu' at Mechanisms and Regulation of Prokaryotic Transcription, FASEB, June 2009
Vandana Kumari, Magda Babina and Margitta Worm, Poster titled ‘Induction of TSLP in murine skin does not require endogenous TNF-α, ADF, March 2013 Received Scholarship for young researchers in 2011 from Dept. of Dermatology, Venereology & Allergology, Charite Campus mitte, Berlin, Germany
Skills BIOCHEMICAL TECHNIQUES
1) Spectrophotometry for quantitative analysis of proteins and DNA.
2) Chromatographic separation of proteins by paper & thin layer chromatography
3) SDS-PAGE 4) In-gel Digestion 5) Peptide Mass Fingerprinting 6) Surface plasmon resonance 7) Western blot MICROBIOLOGICAL TECHNIQUES 1) Gram staining 2) Cell counting methods MOLECULAR BIOLOGY
1) Isolating supercoiled plasmid DNA by conventional method and kit
2) RNA isolation from skin and cell culture 3) Agarose gel electrophoresis 4) Autoradiography 5) In vitro Transcription 6) Protein purification 7) Genetic screening 8) Gel filtration 9) Glutraldehyde cross linking 10) Tryptic digestion
11) V8 protease assay MOUSE TECHNIQUES 1) Mouse handling 2) Injection – IV, IP, ID, SC 3) Blood taking 4) Removal of different organs CELL CULTURE TECHNIQUES 1) Isolation and activation of splenocytes and lymph node cells from mice. 2) Isolation and activation of keratinocytes and mast cell from mouse and human skin 3) Flow cytometry 4) Cell Proliferation assays HISTOLOGY AND IMMUNOHISTOCHEMISTRY TECHNIQUES 1) Cutting of skin samples on the microtome 2) Staining – IHC, H&E 3) Quantification IMMUNOLOGICAL TECHNIQUES 1) Co-immunoprecipitation 2 ELISA GENETIC ENGINEERING TECHNIQUES 1) Gene Cloning 2) RT PCR, Real time PCR & Conventional PCR 3) Restriction Digestion
Computer Experience Application : Microsoft Office
Bio Tool : BLAST (Basic Local alignment Search Tool), Primer Designing
Analysis Tool : Graph pad Prism
Referee’s Prof. Dr. med. M. Worm Allergy Centre Charite, Dept of Dermatology, Vernereology and Allergology Campus Charite Mitte, Charitéplatz 1, D-10117 Berlin Ph.no : +49 (30) 450 518 105 E-mail : [email protected]
Dr. Maria Nassiri Allergy Centre Charite, Dept of Dermatology, Vernereology and Allergology Campus Charite Mitte, Charitéplatz 1, D-10117 Berlin Ph.no : +49 (30) 450 518 314 E-mail : [email protected]
Dr. Magda Babina Allergy Centre Charite, Dept of Dermatology, Vernereology and Allergology Campus Charite Mitte, Charitéplatz 1, D-10117 Berlin Ph.no : +49 (30) 450 518 023 E-mail : [email protected] Prof. Dr. V. Nagaraja Chairman Dept. of Microbiology and Cell biology Indian Institute of Sciences Bangalore- 560012, India Ph.no. : +91-80-2932598; 2360 0668 Email : [email protected]
13.05.2015, Bangalore (Vandana Kumari)