Fumi Tsuno

Tsuno Foods & Rice Fine Chemicals Co., Ltd.

Value added products from rice bran and

its application

Foundation : Feb. 1947

Incorporated : Feb. 1949

President : Fumi Tsuno

Capital : Tsuno Foods=JPY 50 million

Tsuno Rice=JPY 50 million Tsuno Transport.=JPY 15 million

Tsuno Development=JPY 3 million

Employees : about 350

Sales : about JPY 20 billion

Factories : Wakayama, Osaka, Takarazuka, Yashiro, Saitama

R&D : Wakayama, Yashiro

Outline of TSUNO

Hiroshima office

Himeji office

Takarazuka factory

Yashiro factory

Kantou factory(Saitama)

Wakayama factory and head office

Tokyo office

Osaka factory

Factories and Offices in Japan

The Ratio of Nutrient Components

in White Rice to Brown Rice

90% 90%

81%

30% 94%

15%

30%

50%

19%

29%

34%

90%

34%

70%

34%

20%

45%

20%

24%

6.0%

40%

vitamin B1

(0.54 mg)

vitamin B2

(0.060 mg)

energy

(350 Kcal) moisture

(16 g) protein

(6.8 g)

lipid

(2.7 g)

carbohydrate

(74 g)

dietary

fiber

(3.0 g)

ash

(1.2 mg)

calcium

(9.0 mg)

magnesium

(110 mg) phosphorus

(290mg) iron

(2.1 mg)

sodium

(1.0 mg)

potassium

(230 mg)

zinc

(1.8 mg)

manganese

(2.1 mg)

niacin

(6.3 mg)

vitamin B6

(0.45 mg)

vitamin E

(1.4 mg)

folate (27 µg)

White

rice

The figures in parentheses indicate the weight of each component per 100 g of brown rice

rice bran oil

tocopherol

tocotrienol

-oryzanol

ferulic acid

cerebroside

polycosanol

sterol

inositol

phytic acid rice bran protein

Rice Bran

94 % of carbohydrates is

contained in white rice,

and 70 % of lipid in rice

bran.

Valuable components from rice bran

Raw material Performance of antioxidation

About Special Rice Oil

4 Endurance !

5 No bad smell !

6 No bad taste !

non GMO

Allergen-free

Made in Japan

1

2

3

7

8

9

For Long term preservation

For Skin care

For Health

Rice bran oil canola oil Soy bean oil

4. Endurance !

After heating 180℃, 48 hr

Performance of antioxidation

acrolein

propanal Canola oil

Rice bran oil

66ppb

344ppb

1270ppb

287ppb

Analysis of the smell of the oil （Heating at 180℃, 2hr）

Smell Taste

bad

good

CH2 O

O

CH3

Y. Endo et al. (2013) J Am Oil Chem Soc：959 - 964

5. No bad smell ! !

oleic acid linoleic acid linolenic acid Vitamin E γ - Oryzanol

Rice bran oil 45% 35% 1% 1000 ppm 0.2 %

Canola oil 63% 19% 9% 520 ppm 0 %

6. No bad taste !

smell volatile substance

FOOD

Smell and taste mechanism

taste water soluble substances

acrolein propanal

b.P ≒ 60℃ Water soluble

- Oryzanol

Tocotrienol

Vitamin-E

Plant Sterol

Improvement in menopausal symptoms

Improvement in hyperlipemia

Antioxidant

Arteriosclerosis prevention effect

Cholesteremia prevention effect

Antitumor effect

Antioxidant

Cholesterol lowering properties

Cholesterol Lowering effect

Hyperlipemia prevention effect

Active ingredients of Rice Bran Oil

7. For health

Administered

Ingredients

Administered

Dose Pharmacologic Effect Reference

Rice bran oil 60 g ↓TC, LDL-C Suzuki & Oshima ,1970

Rice bran oil 50 g ↓TC, LDL-C, ↑HDL-C Tsuji et al., 1989

Rice bran oil 50 g ↓TC, LDL-C, TG Reghuram et al., 1989

-Oryzanol 300 mg ↓TC, LDL-C, ↑HDL-C Yoshino et al., 1989a,

1989b

-Oryzanol 100 mg ↓TC, LDL-C, Apo B Sasaki et al., 1990

-Oryzanol 300 mg ↓TC, LDL-C, TG, ↑HDL-C Ishihara et al., 1982

Ishihara, 1984

Tocotrienol 200 mg

↓TC, LDL-C, Apo B

↓Lpa, platelet aggregation

Qureshi et al. 1997

Effect of Rice Bran Oil and its Ingredients

on Human Lipid Metabolism

Sugano, Shokuhin Kako Gijutsu, 22(1), 26-30, 2002, in Japanese

Fiscal year 1999 Vegetable oil JAS ranking debrief report, p. 72. Japan Institute of oil inspection Foundation.(2000)

7.9

13.6

12.5

16.5

23.4

21.1

0 5 10 15 20 25

Safflower Oil

Cotton Seed Oil

Soybean Oil

Corn Oil

Rice Bran Oil

Canola Oil

(hrs)

Oxidative Stability of Vegetable Oil (AOM Test)

The longer bar represents the higher the oxidative stability. Rice bran oil showed

the highest oxidative stability among six types of vegetable oils.

The comparison of oxidative stability among six vegetable oils by Active Oxygen Method (AOM)

8. For Long term preservation

Researcher (year) prescription effect

M. Kamimura et al. (1963)1)

30 mg/day

1 week

skin temperature (increase)

blood amount of the skin surface (increase)

M. Kitamura et al. (1977)2)

300 mg/day

1～2 month

Urticaria (improve)

Alopecia (improve)

Pururitus (improve)

R. Hayakawa et al. (1994)3)

100 mg/day

4 month

skin surface lipid (increase)

(1 month later)

1）M. Kamimura et al.：Dermatology and urology , 30, 5, 341-344 (1963) 2）M.kitamura et al.： Journal of New Remedies & Clinics, 26, 11, 2011-2022 (1977) 3）R. Hayakawa et al.： Acta Dermatologica Kyoto, 89, 1, 115-119 (1994)

9. For skin care

・humidity retention improvement ・Roughness improvement

Rice Bran Oil PRO-15 30

Appearance Clear, pleasant to the palate & lovely

flavor

Red brown

semisolid

Color (Lovibond

method)

Yellow 30

Red 3.5

Yellow 40

Red 6.0 N/A

Water & impurities 0.1 0.1 N/A

Acid value 0.1 0.2 3.0

Saponification value 180 - 195 180 - 195 130 - 150

Iodine value 92 - 115 92 - 115 N/A

Unsaponifiables 3.5% 6.0% N/A

Peroxide value 2.0meq/kg 10.0meq/kg 5.0meq/kg

-Oryzanol 0.1 – 0.3%* 1.2 – 1.7% 28.5 – 31.5% *Reference value

The above is the values as of March 2013

RBO, PRO-15, 30

VALUE ADDED PRODUCTS

FROM RICE BRAN

★INOSITOL

★GAMMA-ORYZANOL ★FERULIC ACID

★IP6

★WATER SOLUBLE CONCENTRATES ★RICE MAGNESIUM ★PROTEIN

★OIL SOLUBLE CONCENTRATES

★GLYCOSYL CERAMIDE ★POLYCOSANOL

Antioxidant

Anti-inflammatory effect

Anti-diabetic effect

Anti-allergic effect

Improvement in hyperlipemia

Improvement in menopausal symptoms

Improvement in stress-related disorder Chronic gastritis, irritable colon syndrome, ulcer

Autonomic nerve imbalance

Unique compound found in rice

H3CO

HO

O

O

Gamma Oryzanol

• Antioxidant effect

• Antibacterial effect

• Prevention of discoloration

• Raw material of Vanillin

• Alzheimer disease healing effect

• Prevention of muscular fatigue, hypertention

• Antitumor activity of breast, liver and colon

Polyphenol from rice bran Succeeded in the development of effective

extraction method in cooperation with

Wakayama Prefecture Technology Center

H3CO

HO

OH

O

Ferulic Acid

Phytosterol + Triterpene alcohol: 15%

Tocopherol: 3.0%

Tocotrienol: 3.0%

Oil Soluble Concentrates

• Antioxidant effect

• Hypocholesterolemic effect

• Suppressive effect on hyperlipidemia

• Anticancer effect

OH

OHNH

O

OH

OH

OHO

O

CH2OH

Main component of cerebroside

(composed of 9 to 11 kinds of molecular species)

Glycosyl Ceramide

• Moisture protection to dry skin and improvement

in atopic dermatitis (oral ingestion)

• Whitening ability (suppression of melanin formation )

• Immunostimulating effect

• Antiaging effects

• Anticancer effect

(suppression of induction of colon cancer)

OH

Main component, Octacosanol (C28)

Polycosanol

• Inhibition of cholesterol synthesis

• Reduction in sdLDL

• Antithrombogenic activity

• Improvement effect of instantaneous force and

enduring strength

• Increase in basal metabolism

• Vitamin B group component (Rich in colostrum, Essential vitamin for infant)

• Half sweetness of sucrose

• Prevention of fat accumulation in the liver

• Depression of serum cholesterol

Performance of Vitamin B

Sugar alcohol from rice bran

OH

OH

OH

OH

OH

OH

12 3

4

5

6

Inositol

• pH adjustment effect

• Chelating effect

• Antioxidant action

• Approved as GRAS in the US

Acidic solution from

rice bran O

O

O

P OH

P

PO

HO

O

P OH

O

HO

P OHHO

O

P

O

OHHOOH

HO

OH

OH

O

O

IP6 (Phytic acid / Phytin)

• Defatted rice bran extract

• Rich in physiologically functional ingredients

– dietary fiber, a natural vitamin B group (B1, B2 and B6)

• High proportion of minerals including calcium and

magnesium

• Contains about 30% of a physiological activities substance

IP6 (Phytic acid)

• Antioxidant effect

• Chelating effect

• Free from GMO

Water-soluble concentrates

Equivalent magnesium supplementation quality as that

of other sources

Soluble in acidic solution

Tasteless & Odorless

It mainly exists as magnesium phosphate, and contains

more than 12.0% magnesium.

Rice Magnesium

(Magnesium Phosphate)

The first magnesium extracted

from plant origin

Unique protein from rice bran

carrying health function

• Obtained from defatted rice bran

• Well-balanced amino acid composition

• Different from protein composition of

white rice

Rice Bran Protein

(g/100g edible portion)

Amino acid composition

Amino acid value: 96

(by use of the FAO/WHO/UNU (1985) amino acid

requirement pattern for children of preschool age)

Arg 6.52 Ala 4.08

Lys 3.74 Gly 3.69

His 2.17 Pro 2.83

Phe 3.08 Glu 9.02

Thy 2.19 Ser 3.02

Leu 4.72 Thr 2.47

Ile 2.46 Asp 5.54

Met 1.27 Trp 1.07

Val 3.94 Cys 1.03

Extracted naturally from rice bran

GMO Free, BSE / TSE Free, non allergy

GMP Halal Kosher

High Quality under Strictly

Control Management

ISO 9001 + 14001

Tremendous Possibilities of Products from Rice Bran

Antioxidants

UV Absorbers

Lipid Metabolic Stimulator

Anti-Bacterial Materials

Emulsifier

Liver Function Promoters

Natural Mineral Chelator

Physiologically Functional Oil

Moisturizer

Hair Grower

Emollient

Whitening Agent

Bath Agent

Antiaging