using piezoelectric printing to pattern nanoparticle thinfilmsusing piezoelectric printing to...
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Using Piezoelectric Printing to Pattern Nanoparticle Thinfilms
Jan Sumerel, Ph.D.FUJIFILM Dimatix, Inc.Santa Clara, California
USA
Acknowledgements
• Vanderbilt University– David Wright– Leila Deravi– Sarah Sewell– Aren Gerdon
• University of North Carolina, Chapel Hill– Roger Narayan– Andy Doraiswamy
• NASA Ames– Cattien Nguyen
• Santa Clara University– Angel Islas– John Choy
Nanoscale Engineering"Nanotechnology is the understanding and control of matter at dimensions of roughly 1 to 100 nanometers, where unique phenomena enable novel applications."
(U.S. National Nanotechnology Initiative: www.nano.gov)
Therefore nanoscale engineering is the design, analysis, and/or construction of materials containing nanostructures.
Dimatix Materials Printer
Simple Biosensor
A hybrid device with both inorganic and organic materials
Using Ink Jet Printing as Straightforward Technique for Nanomaterial Thinfilm Production
Drop on DemandmwCNTs
Contact angle determines wettability(drop spread) of mwCNTs
13.10
Contact Angle (º)
Bioinks
•Bacterial Cells•Yeast•Proteins•Nucleic Acids•DNA scaffolds
Piezoelectric Ink Jetting Biological MaterialsAre there obstacles?
• Often aqueous solutions– High surface tension – Water = 72.8 dynes/cm
• Low viscosity – 0.89 – 3.00 centipoise
• “Friendly”surfactants?– CMC
http://serve.me.nus.edu.sg/siggi/marangoni_instability_of_a_water.htm
Water on glycerin
BioinksAre they non-Newtonian fluids?
www.wikipedia.com
What happens to a Fluid in the Shear Field Environment?
Relative sizes of Matter and Order of Magnitude
http://micro.magnet.fsu.edu/cells/index.html
Piezoelectric Inkjet Printing of 3.2 kBplasmid DNA
Repeatability of Ink Jetted Genomic DNA and PCR amplification
Streptavidin Printed in Methyl Cellulose Gel Retains Tertiary Structure
Fourier Transform Infrared Spectroscopy
Cy3 IgG Protein Array
No
DH10B Bacterial Cells
Other Sensor Components
• Quantum Dots• Electroinks
– Conductive Silver Precursor Fluids– PEDOT/PSS– Carbon Nanotubes
Ink Jet Printing Quantum Dots Inks
• Quantum Dots from UT Dots
• TEM from UT Dots• 2.6 nm green
emission• 4.0 nm
yellow/orange emission
Contact Angles of Quantum Dot Inks
2.6 nm 6 mg/mL 4.0 nm 3 mg/mL
Fluid Characteristics After Printing 2.6 nm 6 mg/mL
4.0 nm 3 mg/mL
Quantum Dot Inks on Substrate
Contributions to 3D structure dependent on particle concentration and particle size
Ink Jets Print Conductive Patterns for RFID, Electronics, PCBs, and Displays
• Conductive Silver Precursors• PEDOT/PSS• Carbon Nanotubes
Nanoparticle Polydispersity of ANP Conductive Silver Precursor Fluid as Shown by TEM
254 μm Grid Spacing Matrix55% Silver Conductive Ink
10 pL 1 pL
Waveform Employed for ANP Conductive Silver Fluid Precursor
A. B.
Resulting Conductive Silver Thinfilms on Teslin
Before Annealing After Annealing
Atomic Force Microscopy Shows Silver Nanoparticle Film Feature Sizes on Silicon Wafer
Feature width = 40.6 μmFeature height = 1.6 μ m
Feature Sizes Obtained with ANP Conductive Silver Precursor on Kapton®
Surface Measurements of 1 pL drop
Before annealing
After annealing
A. B.
Resistance Measurements for Commercially Available Conductive Silver Precursors
Cabot Conductive Silver Precursor InkANP Conductive Silver Precursor Ink
Gold Nanoparticle InkApplications in Nanobioengineering
Gold binds to proteins via two different mechanisms
•Cysteine residue•Serine, Threonine residues
Braun, Sarikaya and Schulten, Univ. IL
PEDOT/PSS Array on Glass Wafer
Other Sensor Components
PEDOT/PSS as the Fluid Leaves the Nozzles and Time of Flight
In flight(9.26 m/s)
A. B. C.
Contact Angles of PEDOT/PSS and ANP Silver Ink
A. B. C.Glass Wafer Kapton® Polyimide Teslin synthetic film
Electric Luminescence of Polyflourene printed on Silicon Wafer
Bright Field Dark Field + UV
Using Ink Jet Printing as Straightforward Technique for Nanomaterial Thinfilm Production
Drop on DemandmwCNTs
Contact angle determines wettability(drop spread) of mwCNTs
13.10
Contact Angle (º)
Multiwall Carbon Nanotube Scaffold for DNA
Bright Field DAPI
A B
Self-Assembling Biomaterials• Length scale
– Atoms (10-10)– Molecules (10-10-10-9) – Polymers (10-9)– Viruses (10-8)– Cells (10-5)– Multicellular organisms (10-5-101)
• Polymers– DNA – RNA– Proteins– Lipid bilayers self-assemble into membranes– Higher level organization (protein insertion into
membrane)– Trafficking– Extracellular matrices– Support structures (skeleton, teeth, antlers, husks)
• SECRETIONwww.azonano.com
Harnessing Nature’s Methods to Produce 3D Inorganic Materials
• Diatoms• Glass Sponges• Teeth• Bones
Silaffin of the Cylindrotheca fusiformis diatom
Using Ink Jet Printing for Thinfilm PatterningSilica Precipitating Amine Templates
N N
HN O
HN O
NHO
NHO
N
N
N
N
HN
HN
HN
HN
NH
NH
NH
NH
O
O
O O
O
O
OO
H2N
NH2
H2N
NH2
NH2
NH2
NH2
H2N
Polyamidoamino (PAMAM) Dendrimer
Kroger, N., et al. Science, 1999, 286, 1129.Knecht, M. R., Wright, D. W. Langmuir. 2004, 20, 4728.
H3N S S K K S G S Y
HO3PO OPO3H OPO3H
NH2
NH2
N
NH
NH
n = 4 - 9
S G S K G S K COO
33% G4 PAMAM Dendrimer
39.25785.333.1
Horizontal length (µm)
Vert height (nm)
Contact Angle (º)
100 µm
Stroboscopic View of the Dendrimer Ink Droplets.
Patterned Dendrimers
360 µm
360 µm
96 µm spacing, printed 4x with 35 seconds of lag time in between each printing cycle followed by 2 printing cycles s p a c e d a t 6 4 µ m .
1. 64 µm spacing, printed 2x with no lag time. 2. 56 µm spacing, printed 2x with no lag time.
1.
2.
Dendrimer ReactivityDendrimer Reactivity
• Once printed, we propose a “single spot”reaction vessel, wherein printed NH2 -terminated dendrimers will reproducibly yield concentrated areas of SiO2 nanospheres.
++
+ ++
+ +
+ +
+++
++-Si(OH)-Si(OH)
-Si(OH)
-Si(OH)
-Si(OH)
-Si(OH)-Si(OH)
-Si(OH)
Patterned Silica 160 nm Thinfilm Using Ink Jetted Dendrimers as Biomimetic Catalyst
15 20 25 30 35 400
200
400
600
800
1000
1200
1400
1600
nmol
es o
f sili
ca p
rodu
ced
total area of printed material (mm2)
Post-Si condensation
Pre-Si condensation
Conclusions• Nanoparticle Inks
– Conductive Silver Precursor Fluids
– PEDOT/PSS – Carbon Nanotubes
• Bioinks– Proteins – Nucleic Acids– Scaffolding materials
• Templating Organic Materials– Inorganic/organic thinfilms
• Modern Building Materials based on Biomimetics– Surfaces– Structures