Use of the New LACF

590 Commonwealth Ave.

• The new facility is split into a Barrier and Non Barrier Facility

• Both are considered “clean” of rodent pathogens including noro-virus.

Barrier vs Non-Barrier

• The Barrier is used for the breeding of mouse lines that are not commercially available.

• Once a mouse leaves the Barrier it does not come back.

• The Non-Barrier is used once a mouse is involved in an experiment, surgery, behavioral testing, etc.

• Mice in the Non-Barrier can leave for surgery, behavioral testing, etc. and return.

Non-Barrier

• Besides experimental mice, the Non-Barrier also houses rats, ferrets, gerbils, zebra finch birds, and both mouse and rats for ABSL-2.

• In the Non-Barrier there is also a necropsy room, a surgical suite, cagewash facilities and autoclave facilities.

Necropsy

• Necropsy is to be used only for terminal procedures.

• Neither animals nor people can return to an animal room after being in the Necropsy.

Surgical Suite

• The Surgical Suite consists of 3 rooms.– People and instrument prep room.– Actual surgical room.– Animal prep room, which is also the post op

recovery room. – This animal prep/post op recovery room can

also be used as a procedures room.

Clean and Dirty Caging

• There will be a cart outside the cagewash room for the placement of dirty cages.

• There will be a limited but sufficient amount of clean cages, bottles, tops etc available in each rodent room along with cagecards, colored dots and other materials.

Traffic Flow

• Once again flow is from the cleanest to the dirtiest.

• Start with the Barrier Facility which is of limited access.

• Then the clean side of the Cagewash.• Then the Mouse room and the rat room• Then the bird room & then the ferret room. • The last two rooms to be entered are the ABSL-

2 room, and the gerbil room• And finally the dirty side of the cagewash.

• PLEASE do not go from the dirtier to the cleaner areas.

• Once you have been in the Non-Barrier area you CAN NOT go back into the Barrier.

• Once you have been in the dirty side of the cagewash you should not go back into the animal rooms.

• Once you have been in Necropsy you can not go into another animal room.

• For Entry into hallways– Disposable lab coat– Disposable bonnet– Disposable booties– Disposable gloves– Disposable masks

• For Entry into animal rooms & handling of animals or animal cages.

All of the above, PLUS

Disposable arm covers.

PPE

Gowning

• Leave lab coats, jackets etc in the entry area.

• Put on lab coat, bonnet and mask.

• Put on booties as you cross the imaginary line into the LACF.

• Put on gloves after you put on booties.

THANK YOU!!!!