use of dialysis to separate small and large molecules
DESCRIPTION
Use of dialysis to separate small and large molecules. Gel filtration chromatography. Molecular mass determination by gel filtration chromatography. Some commonly used gel filtration materials. Ion-exchange chromatography with stepwise elution. - PowerPoint PPT PresentationTRANSCRIPT
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Use of dialysis to separate small and large molecules
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Gel filtration chromatography
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Molecular mass determination by gel filtration chromatography
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Some commonly used gel filtration materials
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Ion-exchange chromatography with stepwise elution
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Device for generating a linear concentration gradient
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Molecular formulas of cellulose-based ion exchangers
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Some biochemically useful ion exchangers
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Affinity chromatography
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Covalent linking of ligand to agarose
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Derivatization of epoxy-activated agarose
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Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose
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Experimental arrangement for paper chromatography
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Two-dimensional paper chromatography
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Paper electrophoresis
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Apparatus for slab gel electrophoresis
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Diagram of a disc electrophoresis apparatus
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Polymerization of acrylamide and N,N-methylenebisacrylamide to form a cross-linked polyacryl-amide gel
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SDS-PAGE
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Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE
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General formula of the ampholytes used in isoelectric focusing
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Isoelectric points of several common proteins
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Purification of rat liver glucokinase
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END
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An enzyme-linked immunosorbent assay (ELISA)
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Solubilities of several proteins in ammonium sulfate solutions
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Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type
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Solubility of -lactoglobin as a function of pH at several NaCl concentrations
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Protein crystals
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Agarose gel electrophoretogram of a double helical DNA
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Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs
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Detection of proteins by immunoblotting
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Two-dimensional (2D) gel electrophoresis
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Sedimentation coefficients
in Svedbergs (S) for some
biological materials
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A selection of preparative ultracentrifuge rotors
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Zonal ultracentrifugation
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Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm
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Isopycnic ultracentrifugation
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Physical constants of some proteins