urinary tract system - ju medicine · lactose fermenter o swarming motility ( flagellated ) o...
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Urinary Tract System
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• Early morning midstream urine sample.
• Cultured on Macconkey and blood agar .
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• Small loop 1 µl count colonies* 1000(0.01 ml)• Large loop 10µl count colonies* 100(0.1ml)• 10 ^5 > significant infection.• 10^3-10^5 intermediate infection.• 10^3 < Not significant.• Colony count unite : CFU (colony forming unite).
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(E.coli) 1. Escherichia coli
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2. Klebsiella
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o Gram negative rods , nonlactose fermenter
o Swarming motility ( flagellated )
o Prevent swarming by culturingit on CLED or Macconkey media
Proteus
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Biochemical tests for E.coli
1) kligar sugar iron:
• For E.coli :
Acid/acid (A/A)(Y/Y)
Gas +
No H2S
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Kligler test
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Biochemical tests for E.coli
2) Citrate test :
Negative for E.coli
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Biochemical tests for E.coli
3) Urease test :
Negative for E.coli
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Biochemical tests for E.coli
4) SIM (SULFIDE, INDOLE, MOTILITY) MEDIUM
Indole positiveSulfide (H2S) = negativeMotility =motile
Neg Pos
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identified by :
•Gram negative rods
•odor : grape-like smell.
•Oxidase positive.
•Produce different pigments colors:• Pyocyanine (blue-green)
• Pyoverdin (fluorescent yellow –greenish pigment).
• Pyorubrin (red).
• Pyomelanin (brown).
Pseudomonas aeruginosa
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Pseudomonas aeruginosa on mueller hintion agar
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Blood agar
A- staphylococcus .albus
B- staphylococcus.Aureus
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CLED media Lactose fermentation ( +ve )
staphylococcus spp.
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Test for differentiation of Staphylococcus species
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Catalase test
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MSAMannitol salt agar media
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S.aureus
S.albus
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Bile Esculin Test
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Neiserria
Intra cellular
Extra cellular
Gram
Negative
diplococci
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Neisseria
IntracellularExtracellular
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Haemophilius ducreyi (cancroid)
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Schistosoma haematobium worms
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Schistosoma haematobium egg
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Trichomonas vaginalis parasite
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Treponema pallidum
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Candida Species
Candida
Spp.
C.albicans
C.tropicalis
C.glabrata
C.Krusei
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Candida Spp
• Larger than Bacteria
• Budding
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sabouraud dextrose agar
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To Differentiate between C.albican and other Species
Germ tube[ Serum + candida ]
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2. Chrom agar
C.glabrata : violet (dark pink)
glistering C.albicans : Green
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C.TropicalisBlue
C.Krusei : rough dry pale pink
Chrom agar
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