university of the punjabpu.edu.pk/images/cv/1575363797509.pdf · curriculum vitae of dr. muhammad...

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Name of the Person Concerned: PROF. DR. MUHAMMAD IDREES, Ex-Vice Chancellor Hazara University

CURRICULUM VITAE of

PROF. DR. MUHAMMAD IDREES National Centre of Excellence in Molecular Biology

University of the Punjab

POSTAL ADDRESS: CEMB, 87 West Canal Bank Road, Thokar Niaz Baig, Lahore-53700, Pakistan. Telephone: 042-35293141-45; Fax: 042-35293146; Cell No. 0321-4769212; Email: [email protected]

Personal/Contact Information

1. Name: MUHAMMAD IDREES___________________________

2. Father’s Name: MUHAMMAD UZAIR ___________________________

3. Date of Birth: 16-April-1971

4. Sex/Marital Status: Male/Married

5. Nationality: Pakistani

6. CNIC #: 35202-7391398-3_____________________________________

7. Contact: Land Line: 042-95293141-46 (office)___ Mobile: _03214769212

8. Email: [email protected];[email protected]

9. Present Position: PROFESSOR, National Centre of Excellence in Molecular

Biology, University of the Punjab, Lahore

10. Permanent Address: Village & Post Office Tazagram Tehsil Adenzai, District DIR(L)

Khyber Pakhtunkhwa, Pakistan

11. Mailing address: House No. 158/1, Block-B, Abdalian Cooperative Housing

Society, Lahore.

12. Name & Address of my present employer: Vice Chancellor, University of the

Punjab, Lahore; Ministry of Federal Education and Professional Training.

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a. QUALIFICATIONS:

2008-2015 FRCPath Fellow Royal College of Pathologist (FRCPath) London United Kingdom (UK)-Member No. 20005720.-Division-N/A

2006-2007 Post Doctorate in Molecular Hepatitis, Division of Viral Hepatitis,

Coordinating Centre for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta GA 30333 USA.- Division-N/A

2001 - 2006: Ph. D in Molecular Biology (Molecular Virology), University of the Punjab, Lahore, Pakistan.- Division-N/A

Thesis Title: Genome variability studies in the 5’Non-coding region of Hepatitis C Virus and its clinical implications

1997 - 1999: M. Phil in Molecular Biology (Molecular Bacteriology), University of The Punjab Lahore, Pakistan.-FIRST DIVISION

Thesis Title: Molecular Studies on Multiple Drug Resistant Genes in Mycobacterium tuberculosis.

1993 - 1995: M. Sc. in Zoology (Parasitology/Microbiology), University of Peshawar NWFP, Pakistan.- FIRST DIVISION Thesis Title: The Dilemma of Zoo prophylaxis: Cattle ownership can increase rater tan reduce Malaria in Pakistan.

1995 - 1996: B. Ed (Science), Institute of Education & Research, Peshawar University NWFP, Pakistan. FIRST DIVISION

1991 - 1993: B. Sc. (Zoology, Chemistry, Botany), GPGJC, Peshawar University NWFP,

Pakistan- FIRST DIVISION 1988-1990: HSSC (Pre-medical), Nisar Shaheed College (FBISE-Islamabad) Risalpur-

KPK- 2nd Division 1986-1988: HSC (Science) Government High School Shawa Dir (L) KPK-

FIRST DIVISION

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b. EXPERIENCE IN THE FIELD OF HIGHER EDUCATION at i) Hazara University, Mansehra, ii) Centre of Excellence in Molecular Biology (CEMB) University of the Punjab & iii) Centre for Applied Molecular Biology University of the Punjab at the post graduate level:

POSITIONS HELD:

28 July 2016- 28 July 2019: VICE CHANCELLOR

Hazara University Mansehra, Khyber Pakhtunkhwa

10 June 2020- to Continue: PROFESSOR/Head Division of Infectious Disease National Centre of Excellence in Molecular Biology

(CEMB) University of the Punjab, Lahore.

18 June 2016- to 27 July 2016: PROFESSOR & DIRECTOR Centre for Applied Molecular Biology (CAMB)

University of the Punjab, Lahore.

01 July 2015- 17 June 2016: DIRECTOR Centre for Applied Molecular Biology (CAMB)

University of the Punjab, Lahore.

02 March 2013 – 09 June 2020: ASSOCIATE PROFESSOR/Head Division of Molecular Virology, National Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore.

06 July 2006 – 01 March 2013: ASSISTANT. PROFESSOR/Head Molecular Virology & Molecular Diagnostics Groups, National Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore.

01 December 2005 – 05 July 2006: SENIOR RESEARCH OFFICER/ Head Molecular Diagnostics, Centre for Applied Molecular Biology (CAMB) Ministry of Science & Technology, Lahore.

01 June 2000 – 30 November 2005: RESEARCH OFFICER/Lab Incharge Centre for Applied Molecular Biology (CAMB) Lahore.

January 1999-April 2001: Head, Division of Molecular Diagnostics The Medical Laboratories 34- Lawrence Lahore

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OTHER APPOINTMENTS AND AFFILIATIONS (CURRENT & PAST)

A. Fellow/Memberships of International Societies:

1. Fellow Royal College of Pathologist (FRCPath) London United Kingdom (UK)-Member No. 20005720

2. American Society of Microbiology (ASM)-Member ID: 56817638 3. European Society of Clinical Microbiology and Infectious Diseases

(ESCMID)- Member ID: 122577 4. Zoological Society of Pakistan 5. Hellenic Society of Virology 6. International Society for Antiviral Research 7. The Federation of European Microbiological Societies

B. Memberships of National Bodies: 8. Member Senate, Hazara University 9. Member senate, Women University Mardan 10. Member Senate, University of Baltistan, Gilgit Baldistan 11. Convener/Chairman, Hazara University Syndicate 12. Member Syndicate, University of Malakand 13. Convener/Chairman, Hazara University Selection Board 14. Convener/Chairman, Academic Council Hazara University Mansehra-KP 15. Convener/Chairman, Board of Faculties (BOF), Hazara University

Mansehra-KP 16. Convener/Chairman, Board of Studies (BOS), CAMB, University of the

Punjab Lahore. From 01-07-2015 to 27-07-2016. 17. Member Board of Studies (BOS) CAMB, University of the Punjab Lahore.

From 28-08-2016-continue 18. Member, Board of Faculty (BOF), University of the Punjab Lahore. From 01-

07-2015 to 27-07-2016. 19. Member Board of Studies (BOS) Department of Genetics, Hazara University

Garden Campus Mansehra, KPK-From 2011-2015 20. Member, Academic Council University of the Punjab Lahore. From 22-8-

2015-27-07-2016. 21. Member National Quality Assurance Committee (NQAC), Higher Education

Commission of Pakistan-From December 2016-July 2019.

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22. Member Cabinet Committee on Dengue Control-Health Department Constituted by Chief Minister, Government of the Punjab. From 2012-till date

23. Member (Co-opted) of “Technical Expert Committee of Medicine” recommended by Steering Committee on Prevention & Control of Hepatitis Program. From 2013-till date

24. Member Guidance & Examination Committee of Atta ur Rehman School of Applied Biosciences (previously known as NCVI) National University of Science & Technology (NUST) Islamabad- From 2011- continue

25. Member Board of Studies (BOS) Department of Molecular Biology, Virtual University of Pakistan M. A. Jinnah Campus Lahore. From February 2016-continue

26. Group Leader, Infectious Diseases & Molecular Virology Group CEMB, University of the Punjab- From 2000- continue

27. Incharge Disease Diagnostic Facility, CAMB, Ministry of Science & Technology Government of Pakistan- From 2001- 2009.

28. Member of a team of four Scientist designated by CDC, USA to visit Pakistan (2006-07) to meet with several public health officials from the Ministry of Heath and the National Institute of Health to discuss various collaborative public health programs and activities that will support the Field Epidemiology & Laboratory Training Program.

29. Member of Expert Committee for the preparation of guidelines of MERS CoV constituted by Advisor to Chief Minister on Health-2014.

30. Member of Departmental Tenure Review Committee (DTRC) of CEMB, University of the Punjab Lahore. From 2013-till date

31. Member/Virology Group Leader of Dengue Research Group (DRG) constituted by Vice Chancellor, University of the Punjab for the prevention and control of Dengue. From 2009-till date.

32. Member Biosafety & Bioresource Committee University of the Punjab to review the research proposals of the faculty members and Ph. D scholars before submission to Higher Education Commission (HEC) and Pakistan Science Foundation (PSF). From 22-9-2015-ongoing.

33. Chairman/Convener, Purchase Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015- 2016

34. Chairman/Convener, Technical Evaluation Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015- 2016

35. Chairman/Convener, Departmental Doctoral Program Committee (DDPC), Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015-2016

36. Chairman/Convener, Scholarship Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015-2016

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37. Reviewer of more than 20 International Scientific Journals (few are given below):

The Lancet Infectious Diseases Nature Emerging Infectious Diseases Virus Genes European J. Virological Methods, BMC Virology, BMC Infectious Diseases, Infection, Genetics, Evolution, Journal of Medical Virology

38. Reviewer of grants in the field of Health Biotechnology for Higher Education Commission (HEC) of Pakistan.

39. Focal person Higher Education Commission (HEC) of Pakistan for grants in the field of Health Biotechnology-2016-2017.

40. Visiting Scientist of:

a. CDC, US Department of Health & Human Services, Atlanta, USA. b. Ministry of Health Government of the Punjab. c. Lahore General Hospital Lahore, Pakistan d. Peshawar Medical Collage, Peshawar, NWFP, Pakistan e. Dow University of Health Sciences, Karachi f. Liaquat University of Medical & Health Sciences Jamshoro Sindh.

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c. PUBLICATIONS RECORD: Web of Science ID: K-6032-2012 Web

of Science: https://publons.com/onboard/?onboard_type=rid#rid-intro

GoogleScholar: https://scholar.google.com.pk/citations?user=MZuiNDIAAAAJ&hl=en

Dr. Idrees has a distinguished research and Excellent Publications record and his contribution in Molecular Biology has been recognized at home and abroad. His research work has been published in more than 205 peer reviewed research/review papers in national/international scientific journals with an overall impact factor more than 400 and of citation of more than 4’800. Brief and publication list is given below:

(A) Accumulative Impact Factor: 411.826; (updated on 01/07/2020) (B) Accumulative Citations: 4’808 (Google Scholar)

2’913 (Web of Science) Total Publications: 205 As Corresponding Author: 88 Publications As First Author: 19 Publications As Contributing Author: 98 Publications

LIST OF RESEARCH PAPERS PUBLISHED IN JOURNALS

(With Impact Factor and Citations)

Proforma for Calculation of Impact Factor (Through web of knowledge)

Author's Research Title (Idrees M)

SR. NO. JOURNAL IMPACT FACTOR

CITAT IONS

1. Publications in year 2008 (Impact Factor 6.386) Idrees M*, Development of a New HCV Genotyping Assay and its Suitability for the Detection of Common Genotypes in Hepatitis C Patients in Pakistan. Journal of Virological Methods. 4 March 2008; 150(1): 50-56. IF 1.746

1.786

72

2. Idrees M*, Riazuddin S. Frequency Distribution of Hepatitis C Virus Genotypes in Different Geographical Regions of Pakistan and their Possible Routes of Transmission, BMC Infectious Diseases. 23 May 2008; 8: 69. IF 2.951

2.688 288

3. Idrees M*, Riazuddin S. High Prevalence of Hepatitis C Virus Infection in the Largest Province of Pakistan. Journal of Digestive Diseases. 05 September 2008; 9: 96-104. IF 1.689

1.736 108

4. Publications in year 2009 (Impact Factor 10.288)

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Idrees, M*, Riazuddin S. A study of best positive predictors for sustained virologic response to interferon alpha plus ribavirin therapy in naive chronic hepatitis C patients. BMC Gastroenterology. 20 January 2009, 9:5. doi:10.1186/1471-230X-9-5. IF 2.694.

2.694 121

5. Idrees M*, Butt S, Awan Z, Aftab M, Khubaib B, Rehman IU, Akram M, Mamzoor S, Akbar H, Rafique S, Riazuddin S. Nucleotide identity and variability among different Pakistani hepatitis C virus isolates. Virology Journal. 24 August 2009; 6: 130. doi:10.1186/1743-422X-6-130. IF 2.759

2.759 16

6. Idrees, M*, Rafique S, Rehman I, Butt S, Riazuddin S. Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience. World Journal of Gastroenterology. 15 October 2009; 15(40): 5080-5085. IF 5.130

5.130 93


Afzal S, Idrees M*, Akram M, Awan Z. Mutations in the E2-PePHD region of hepatitis C virus genotype-3a and correlation with response to interferon and ribavirin combination therapy in Pakistani patients. Virology Journal. 31 December 2010; 7: 377. IF 2.759

2.759

16

8. Butt AM, Siddique S, Idrees M*, Tong Y. Avian influenza A (H9N2): computational molecular analysis and phylogenetic characterization of viral surface proteins isolated between 1997 and 2009 from the human population. Virology Journal. 15 November 2010; 7: 319. IF 2.465

2.759 71

9. Ali A, Ahmed H, Ali I, Khan S, Zaidi G and Idrees M, Prevalence of active Hepatitis C Virus infection in District Mansehra Pakistan'. Virology Journal. 22 November 2010; 7: 334. IF 2.465

2.759 45

10. Munir S, Saleem S, Idrees M*. Hepatitis C Treatment: current and future perspectives. 2010. Virology Journal. 1 November 2010; 7: 296. IF 2.465

2.759 145

11. Zaidi G, Idrees M*, Awan Z, Amin I, Shahid M, Younas S, Hussain R. Prevalence of hepatitis delta virus infection among hepatitis B virus surface antigen positive patients circulating in the largest province of Pakistan. Virology Journal. 26 Oct 2010; 7: 283. IF 2.465

2.759 31

12. Ali A, Ahmed H, Idrees M*, Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan. Virology Journal. 26 August 2010; 7: 203. (doi:10.1186/1743-422X-7-203). IF 2.465

2.759 64

13. Butt S, Idrees M*, Akbar H, Awan Z, Rehman IU, Hussain A, Afzal S, Shahid M, Manzoor S, Rafique S. The changing Epidemiology pattern and Frequency Distribution of hepatitis C virus in Pakistan. Infection, Genetics and Evolution. 9 May 2010; 10(4): 595-600. IF 2.591

2.591 51

14. Chaudhary S, Idrees M*, Butt AK, Khan AA, Izhar M, Iqbal 1.595 23

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HA. Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples. Current Microbiology. 26 April 2010; 14(6): 344-347. IF 1.595

15. Awan Z, Idrees M*, Amin I, Butt S, Afzal S, Akbar H, Rehman IU, Younas S, and Shahid M. Pattern and Molecular Epidemiology of Hepatitis B virus genotypes circulating in Pakistan. Infection, Genetics and Evolution. 2010; 10(6): 345-350. IF 2.591

2.591 41

16. Rafique S, Idrees M*, Nasim A, Akbar H, Athar A. Transition metal complexes as potential therapeutic Agents. Biotechnology and Molecular Biology Reviews. 16 April 2010; 5(2): 38-45.

284

17. Rafique S, Nasim A, Idrees M. Riazuddin S. Stable expression of envelope glycoproteins of HCV genotype 3a in Huh-7 cell-lines. Int. J. Appl. Biol. Pharma. Tech. Aug-Sep 2010; 1(2): 199-213. IF 0.4

0.4 01


Idrees M*. Rehman IU, Manzoor S. Akbar H. Butt S. Evaluation of three different hepatitis C virus typing methods for detection of mixed‐genotype infections. Journal of Digestive Diseases. 2011, 12: 199-203. IF 1.887

1.887

16

19. Idrees M*, Lal A, Malik FA. Occult hepatitis C virus infection and associated predictive factors: the Pakistan experience. Infection, Genetics and Evolution. 2011; 11(2): 442-45. IF 3.264

3.264 28

20. Butt, S., Idrees M*, Rehman IR, Hussain A. Mixed genotype infections with hepatitis C Virus, Pakistan. Emerging Infectious Diseases. 2011; 17(8) 1565-1567. IF 7.422

7.422 18

21. Khan AU, Waqar M, M, Zaib M, Wasim M, Idrees M. Bajwa MA. True prevalence of Twin HDV-HBV infection in Pakistan: A Molecular approach. Virology Journal 2011, 8:420. IF 2.465

2.759 32

22. Ali A, Nisar M, Ahmad A, Idrees M*, Saif N, Bajwa MA. Determination of HCV genotypes and viral loads in chronic HCV infected patients of Hazara Pakistan Virology Journal 2011, 8:466. IF 2.465

2.759 51

23. Manzoor S, Idrees M*, Ashraf A, Mehmood A, Butt S, Fatima K, Akbar H, Rehman IU, Qadri Q. Identification of ionotrophic purinergic receptors in Huh-7 cells and their response towards structural proteins of HCV genotype 3a. Virology Journal. 2011, 8: 431 doi:10.1186/1743-422X-8-431. IF 2.465

2.759 16

24. Fatima Z, Idrees M*, Bajwa MA, Tahir Z, Ullah O, Zia MQ, Hussain A, Akram M, Khubaib B, Afzal S, Munir S, Saleem S, Rauff B, Badar S, Naudhani M, Butt S, Aftab M, Ali L, Ali M. Serotype and genotype analysis of Dengue Virus by Sequencing followed by Phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan. BMC Microbiology. 2011, 11: 200. IF 3.322

3.322 86

10


25. Khan F, Akbar H, Idrees M, Khan H, Shahzad K, Kayani MA. The prevalence of HBV infection in the cohort of IDPs of war against terrorism in Malakand Division of Northern Pakistan Journal: BMC Infectious Diseases. 2011, 11:176. IF 2.69

2.69 29

26. Riaz M, Idrees M, Kanwal H, Kabir F. An overview of Triple infection with Hepatitis B, C and D viruses. Virology Journal. 2011, 8: 368. IF 2.465

2.759 02

27. Yousaf MZ, Idrees M, Saleem MZ, Ali M, Expression of core antigen of HCV genotype 3a and its evaluation as screening agent for HCV infection in Pakistan. Virology Journal. 2011, 8: 364. IF 2.465

2.759 13

28. Faridi R, Zahra A, Idrees M*, Khan K. Oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer. Virology Journal. 2011, 8: 269. IF 2.465

2.759 245

29. Ali I, Khan S, Attaullah S, Khan SN, Khan J, Siraj S, Iqbal A, Swati ZA, Idrees M. Response to combination therapy of HCV 3a Infected Pakistani patients and the role of NS5A protein. Virology Journal. 2011, 17: 8(1): 258. IF 2.465

2.759 19

30. Butt AM, Siddique S, Tahir S, Nasrullah I, Hussain M, Idrees M*, Lu J, Tong Y. Comparative sequence, antigenic and phylogenetic analysis of avian influenza (H9N2) surface proteins isolated in Pakistan between 1999 and 2008. J Infect Dev Ctries 2011; 5(6):413-424. IF 1.67

1.67 06

31. Akram M, Idrees M*, Hussain A, Afzal S, Characterization of Hepatitis C Virus genotype 3a Hypervariable region 1 in patients achieved rapid virological response to alpha interferon and Ribavirin Combination therapy. Virology Journal. 2011, 17: 8(1): 253. IF 2.465

2.759 04

32. Akram M, Idrees M*, Zafar S, Hussain A, Butt S. Effects of host and virus related factors on interferon-α+ ribavirin and pegylated-interferon+ ribavirin treatment outcomes in chronic Hepatitis C patients. Virology Journal. 2011, 17: 8(1): 234. IF 2.465

2.759 23

33. Rafique S, Idrees M*, Positional effect of phosphorylation sites 266 and 267 in the cytoplasmic domain of the E2 protein of hepatitis C virus 3a genotype: Interferon Resistance analysis via Sequence. Virology Journal. 2011, 8: 204. IF 2.465

2.759 06

34. Akbar H, Idrees M*, Butt S, Sabar FM. High Baseline Interleukine-8 level is an Independent Risk Factor for the achievement of Sustained Virological Response in Chronic HCV Patients. Infection, Genetics and Evolution. 2011; 11(6): 1301-1305. IF 2.591

2.591 26

35. Ali M, Idrees M*. Hepatitis B virus in Pakistan: a systemic review of prevalence, risk factors, awareness status and genotypes. Virology Journal. 2011, 8; 102. IF 2.465

2.759 161

36. Rauff B, Idrees M*. Hepatitis Associated Aplastic Anemia: A review. Virology Journal 2011, 8: 87. IF 2.465

2.759 126

37. Iqbal T, Idrees M*. Isolation and characterization of two new 2.759 09

11


Hepatitis E Virus Genotype 1 strains from two Mini-outbreaks in Lahore, Pakistan. Virology Journal. 2011, 8: 94. IF 2.465

38. Afzal S, Idrees M*, Ali M, Ilyas M, Akram M, Butt S, Salem S, Hussain A, Rehman IU, Ali L, Shahid M. Envelope 2 protein phosphorylation sites S75 & 277 of hepatitis C virus genotype 1a and interferon resistance: A sequence alignment approach. Virology Journal. 2011, 8:71. IF 2.465

2.759 15

39. Shehzadi A, Rehman SU, Idrees M*. Promiscuous prediction and conservancy analysis of CTL binding epitopes of HCV 3a viral proteome from Punjab Pakistan: an In Silico Approach. Virology Journal. 2011, 8:55. IF 2.465

2.759 19

40. Shah SAR, Idrees M*, Hussain A. Hepatitis G virus associated aplastic anemia: a recent case from Pakistan. Virology Journal. 2011, 8 (1): 30. IF 2.465

2.759 19

41. Ali L, Idrees M*. An overview of treatment response rates to various anti-viral drugs in Pakistani Hepatitis B Virus infected patients. Virology Journal. 2011, 8: 20. IF 2.465

2.759 12

42. Ullah I, Idrees M*, Ahmad H, Ghafoor SU, Ali M, Ali L, Ahmad A Hepatitis C virus genotypes circulating in district Swat of Khyber Pakhtoonkhaw, Pakistan. Virology Journal. 2011; 8:16. IF 2.465

2.759 40

43. Butt S, Idrees M, Amin I, Younas S, Afzal S, Akbar H, Rehman IU. Change in hepatitis C virus clades: A cross-sectional study of chronic HCV patients in Pakistan from 2000–2010. European Journal Clinical Microbiology and Infection. 2011; 30: 669-672 IF 2.857

2.857 04

44. Chaudhry S, Khan AA, Butt AK, Idrees M. Helicobacter pylori in Dental Plaque; Is it Related to Brushing Frequency, Plaque Load and Oral Health Status? JCPCP 2011, 21(10): 589-592. HEC W-category IF 0.407

0.407 15

45. Butt, S., Idrees M*., Rehman IR, Ali L, Hussain A, Ali M, Ahmad N. Establishment of stable Huh-7 cell lines expressing various Hepatitis C Virus Genotype 3a protein: An in-vitro testing system for novel anti-HCV drugs. Genetic Vaccines and Therapy 2011, 9:12. IF 1.71

1.71 07

46. Rehman IU, Idrees M*, Ali M, Ali L, Butt S, Hussain A, Akbar H, Afzal S. Hepatitis C virus genotype 3a with phylogenetically distinct origin is circulating in Pakistan Genetic Vaccines and Therapy 2011, 9:2. IF 1.71

1.71 33

47. Butt AM, Siddique S, Tahir S, Nasrullah I, Hussain M, Idrees M*, Lu J, Tong Y. Comparative sequence, antigenic and phylogenetic analysis of avian influenza (H9N2) surface proteins isolated in Pakistan between 1999 and 2008. Emerging Problems in Infectious Diseases JIDC. 2011; 5(6):413-424. IF 1.353

1.353 06


Idrees M, Hussain W, Rehman HU, Tayyab GN. Dengue Virus

20

12


Serotype 2 (DEN-2): the Causative Agent of 2011-Dengue Epidemic in Pakistan. American Journal of Biomedical Sciences. 2012; 2012(4): 307-315. doi: 10.5099/aj120400307 ICV=5.74

49. Butt AM, Feng D, Nasrullah I, Tahir S, Idrees M, Yigang Tong Y, Lu J. Computational identification of interplay between phosphorylation and O-β-glycosylation of human occludin as potential mechanism to impair hepatitis C virus entry. Infection Genetics Evolution. 2012 Aug; 12(6):1235-1245. IF 2.611

2.611 15

50. Shah AR, Idrees M, Hepatitis E virus-associated aplastic anaemia: the first case of its kind. Journal of Clinical Virology. 2012; 54: 96-97. IF 2.647

2.647 53

51. Butt A, Feng D, Idrees M, Tong Y, Lu J. Computational identification and modeling of crosstalk between Phosphorylation, O-β-glycosylation and Methylation of FoxO3 and implications for cancer therapeutics. International Journal of Molecular Science 2012; 13(3): 2918-2938; doi:10.3390/ijms13032918. IF 4.183

4.183 13

52. Badar S, Khubaib B, Idrees M. et al., Association of Hepatitis C Virus With Insulin Resistance: Evidences From Animal Studies and Clinical Studies. Hepatitis Monthly. 2012; 12(1): 11-15. IF 1.932

1.932 06

53. Ali A, Nisar M, Idrees M*, Ahmad H. Prevalence of HBV infection in suspected population of conflict-affected area of war against terrorism in North Waziristan FATA Pakistan. Infection, Genetics and Evolution. 12(2012); 1865-1869. IF 2.611

2.611 12

54. Butt AM, Tahir S, Nasrullah I, Idrees M*, Lu J, Tong Y. Mycoplasma genitalium: a comparative genomics study of metabolic pathways for the identification of drug and vaccine targets. Infection Genetics and Evolution. 2012 Jan;12(1): 53-62. IF 2.611

2.611 38

55. Butt AM, Khan IB, Hussain M, Idrees M*, Lu J, Tong Y. Role of post translational modifications and novel crosstalk between phosphorylation and O-beta-GlcNAc modifications in human claudin-1, -3 and -4. Molecular Biology Reports. 2012 Feb; 39(2):1359-1369 IF 2.107

2.107 21

56. Publications in year 2013 (Impact Factor 10.048) Hussain A, Idrees M. The first complete genome sequence of HCV-1a from Pakistan and a phylogenetic analysis with complete genomes from the rest of the world. Virology Journal. 2013, 10: 211. IF 2.465

2.759

07

57. Ullah S, Rehman HU, Idrees M. Mutations in the NS5A gene are associated with response to interferon+ ribavirin combination therapy in patients with chronic hepatitis C virus 3a infection. European Journal of Gastroenterology& Hepatology. 2013 Oct; 25(10):1146-1151. IF 2.093

2.093 04

58. Ali A, Rehman HU, Nisar M, Rafique S, Ali S, Hussain A, 3.538 40

13


Nausheen, Idrees M. Seroepidemiology of dengue fever in Khyber Pakhtunkhawa, Pakistan International Journal of Infectious Diseases 2013, 17 (7): e518-e523. doi: 10.1016/j.ijid.2013. 01.007. Epub 2013 Mar 21. IF 3.538

59. Fatima Z, Afzal S, Idrees M. Change in demographic pattern of dengue virus infection: evidence from 2011 dengue outbreak in Punjab, Pakistan. Public Health, 2013; Sep; 127(9): 875-877. IF 1.952

1.952 17


Afzal S, Idrees M, Hussain M. De Novo modeling of Envelope 2 protein of HCV isolated from Pakistani patient and epitopes prediction for vaccine development. Journal of Translational Medicine. 2014; 12:115. doi:10.1186/1479-5876-12-115. IF 4.301

4.301

05

61. Butt A, Idrees M, Tong Y. Identification and expression profiling of microRNAs in hepatitis C patients by using next generation sequencing. Journal of Viral Hepatitis. Vol 21 (Suppl. S2), October 2014, 21–48. IF 4.179

4.179 0

62. Ali M. Rehman IU, Idrees M. Emergence of genetically variant Hepatitis C virus population in response to increased antiviral drug pressure, Pakistan. Virus Genes. June 2014, Volume 48, Issue 3, pp 543-549. IF 1.285

1.285 07

63. Shahid M, Idrees M, Nasir B, Raja AJ, Raza SM, Amin I, Rasul A, Tayyab GU. Correlation of biochemical markers and HCV RNA titers with fibrosis stages and grades in chronic HCV-3a patients. European Journal of Gastroenterology Hepatology. 2014 Jul;26(7):788-94. doi: 10.1097/MEG.0000000000000109. IF 2.198

2.198 17

64. Sabri S, Idrees M. Studies on the role of NS3 and NS5A non-structural genes of hepatitis C virus genotype 3a local isolates in apoptosis. International Journal of Infectious Diseases. 2014, 25(2014): e38-e44. IF 3.538

3.538 07

65. Rafique S, Idrees M, Ali A and Iqbal M. Generation of infectious HCV pseudo typed particles and its utilization for studying the role of CD81 & SRBI receptors in HCV infection. Molecular Biology Reports. June 2014, Volume 41, Issue 6, pp 3813-3819; IF 2.107

2.107 01

66. Rafique S, Idrees M, Ali A and Iqbal M. Studies on the role of neutralizing antibodies against envelope genes in resolving HCV pseudo-particles infection. Molecular Biology Reports. June 2014, Volume 41, Issue 6, pp 3945-3950. DOI 10.1007/s11033-014-3262-y. IF 2.107

2.107 01

67. Waqar M, Khan AU, RehmanHU, Idrees M*, Wasim M, Ali A, Niaz Z, Ismail Z, Rehman MU, Tariq M. Determination of Hepatitis C Virus Genotypes Circulating in Different Districts of Punjab (Pakistan). European Journal Gastroenterology & Hepatology. 2014 Jan; 26(1): 59-64. doi:

2.198 27

14


10.1097/MEG.0b013e328362dc3f, IF 2.198 68. Butt, FA, Amin I, Idrees M, Iqbal M. Hepatitis delta virus

genotype-1 alone cocirculates with hepatitis B virus genotypes A and D in Pakistan. European Journal of Gastroenterology & Hepatology. 2014 Mar; 26(3): 319-324. doi: 10.1097/MEG.0000000000000007. IF 2.198

2.198 09

69. Ur Rehman I, Vaughan G, Purdy MA, Xia GL, Forbi JC, Rossi LM, Butt S, Idrees M, Khudyakov YE. Genetic History of Hepatitis C Virus in Pakistan. Infection, Genetics and Evolution. 2014 Aug 13; 27C: 318-324. IF 2.611

2.611 05


Idrees M, Shah AR. Association of aplastic anaemia with positive viral hepatitis serology, and its outcome after immunosuppressive therapy. Journal of Clinical Virology August 2015, Volume 69, page 241. IF 2.647

2.647

71. Nasrullah I, Butt AM, Tahir S, Idrees M, Tong Y. Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Marburg virus evolution. BMC Evolutionary Biology 2015, 15:174. doi:10.1186/s12862-015-0456-4. IF 3.407

3.407 40

72. Akram M, Fatima Z, Purdy MA, Sue A, Saleem S, Amin I, Shahid M, Nawaz R, Idrees M. Introduction and evolution of dengue virus type 2 in Pakistan: a phylogeographic analysis. Virology Journal (2015) 12: 148; IF 2.465

2.759 14

73. Butt AM, Castellano L, Idrees M. Next generation sequencing reveals differential expression profiles of hepatic microRNAs in chronic hepatitis C patients. Journal of Hepatology. 2015 vol. 62: S585. IF 18.946

18.946 0

74. Butt AM, Castellano L, Idrees M, Tong Y. Deep sequencing shows microRNA expression in hepatitis C patients is associated with the end treatment response. Journal of Clinical Virology. Aug 2015; Volume 69, Page 234. IF 3.020

3.020 0

75. Amat-ur-Rasool H, Saghir A, Idrees M, Computational Prediction and Analysis of Envelop Glycoprotein Epitopes of DENV-2 and DENV-3 Pakistani isolates: A First Step towards Dengue Vaccine Development. PLOS ONE. 2015 Mar 16; 10(3): e0119854. doi: 10.1371/journal.pone.0119854. eCollection 2015. IF 3.534

3.534 17

76. Khubaib B, Idrees M, Afzal S, Wasim M. The genotype CC of IL‐28B SNP rs12979860 is significantly associated with a sustained virological response in chronic HCV‐infected Pakistani patients. Journal of Digestive Diseases. 2015; 16; 293–298. IF 1.887

1.887 18

77. Ali A, Idrees M. Expression of Hepatitis C Virus Core and E2 antigenic recombinant proteins and their use for development of diagnostic assays. International Journal of Infectious Diseases. Mar 2015; 84 (2015): e84-e89. IF 3.538

3.538 07

15


78. Hussain M, Idrees M, Afzal S. Development of Global Consensus of Dengue Virus Envelope Glycoprotein for Epitopes based vaccine design. Current Computer-Aided Drug Design. 2015; 11(1): 84-97. IF 1.155

1.155 06

79. Hussain A, Idrees M, Asif M, Ali L, Rasool M. Phylogenetic and 2D/3D Analysis of HCV 1a NS4A Gene/Protein in, Pakistani Isolates. Hepatitis Monthly.. 2015 June; 15(6): e19936. IF 1.932

1.932 01

80. Afzal S, Maqbool A, Zaman S, Idrees M, Anjum AA, Shah SS, Fatima Z, Amin A and Latif M. Evidence of Transmission of Dengue Virus by Different Developmental Stages of Mosquitoes, Aedes aegypti and Aedes albopictus. Pakistan Journal of Zoology., 2015; vol. 47(3), pp. 887-890. IF 0.79

0.79 02

81. Shah SS, Maqbool A, Zaman S, Idrees M, Anjum AA, Afzal S,, Fatima Z, Amin A and Ahmad S. Role of Aedes and Culex in Dissemination of Dengue Virus. Pakistan Journal of Zoology., 2015; vol. 47(1), pp. 273-276. IF 0.79

0.79 01

82. Shaheen MA, Idrees M. Evidence-based consensus on the diagnosis, prevention and management of hepatitis C virus disease. World Journal of Hepatology. 2015 March 27; 7(3): 616-627. IF 1.206

1.206 29


Butt A, Raja AJ, Siddique S, Khan JS, Shahid M, Tayyab GN, Minhas Z, Umar M, Idrees M, and Tong Y. Parallel expression profiling of hepatic and serum microRNA-122 associated with clinical features and treatment responses in chronic hepatitis C patients. Nature Scientific Reports 6, Article number: 21510 (2016). IF 5.228

5.228

18

84. Shan MA, Hussain M, Shafique M, Shahzad M, Parveen R, Idrees M. Genetic distribution of 15 autosomal STR markers in the Punjabi population of Pakistan. International Journal of Legal Medicine. 2016, Nov; 130(6):1487-1488. IF 2.862

2.862 05

85. Akram M, Idrees M. Complete genome sequencing and comparative analysis of three dengue virus type 2 Pakistani isolates. VirusDisease March 2016, Volume 27, Issue 1, pp 27-33. IF 0.364

0.364 01

86. Khubaib B, Idrees M, Hussain A. Construction of Mammalian Expression Vector of Core Gene of HCV of Pakistani Isolate Genotype 1a. J Hum Virol Retrovirol April, 2016, 3(3): 00089. IF 1.974

1.974 0

87. Wahid B, Ali A, Rafique S, Idrees M. Zika: As an emergent epidemic. Asian Pacific Journal of Tropical Medicine August, 2016; 9(8): 723–729. IF 1.772

1.772 21

88. Yi M, Athar MA, Wu, Y, Xu Y, Wu J, Xu Z, Hayder Z, Saeed Khan, Idrees M, Nasir MI, Liao Y, Li Q. Detection of anti-HBV Drug Resistance Mutations Based on Multi-color Melting Curve Analysis. Journal of Clinical Microbiology. Nov 2016; 54(11):

4.232 02

16


2661-2668. IF 4.232 89. Wahid B, Idrees M, Rafique S. Immunotherapeutic strategies for

sexually transmitted viral infections: HIV, HSV and HPV. Cellular Immunology. Dec 2016; 310:1-13. IF 3.291

3.291 02

90. Mirza MU, Rafique S, Ali A, Munir M, Ikram M, Manan A, Salo-Ahen OMH, Idrees M. Towards peptide vaccines against Zika virus: Immunoinformatics combined with molecular dynamics simulations to predict antigenic epitopes of Zika viral proteins. Nature Scientific Reports Dec 2016; 6, Article number: 37313. IF 5.228

5.228 60

91. Ali A, Ahmad H, Idrees M, Zahir F, Ali I, Circulating serotypes of dengue virus and their incursion into non-endemic areas of Pakistan; a serious threat. Virology Journal. (2016) 13:144. IF 2.465

2.465 16

92. Hussain A, Awan Z, Idrees M. Influence of Hepatitis B virus genetic heterogeneity on treatment response against Lamivudine and Interferon in Pakistani isolates. Anti-Infective Agents, 2016, 14(2): 121-125. IF 1.08

1.08 0

93. Ali M., Idrees M., A systematic review of treatment response rates in Pakistani hepatitis C virus patients; Current prospects and future challenges. Medicine. 2016; 95: 50. IF 2.133

2.133 07

94. Younas S, Amin I, Shahid M, Fatima Z, Idrees M. Detection of Salmonella enterica, Subsp. enterica Serovar Typhi in Dengue Virus Seropositive Samples. Pakistan Journal of Zoology. Vol. 2016; 48(6), pp. 1741-1746, 2016. IF 0.79

0.79 0

95. Amin I, Naz S, Shahid M, Idrees M. Inhibitory Effects of Thiazolide Compounds against Hepatitis C virus: in vitro Study. International Journal of Virology and Molecular Biology. 2016, 5(2): 27-33

0

96. Khalil TA, Ali M, Tanveer F, Ovais M, Idrees M, Shinwari ZK, Hollenbeck JE. Emerging Viral Infections in Pakistan: Issues, Concerns, and Future Prospects. Health Security, Volume 15, Number 3, 2017, DOI: 10.1089/hs.2016.0072. IF 1.91

1.91 11


Khan K, Siddiqi MH, Abbas M, Almas M, Idrees M. Forensic Applications of Y chromosomal properties. Legal Medicine, May 2017; Volume 26, Pages 86–91. IF 1.404

1.404

04

98. Ali M, Khan T, Fatima K, Ali QA, Ovais M, Khalil AT, Ullah I, Raza A, Shinwari ZK, Idrees M. Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions. Phytotherapy Research. Oct 2017; 31(11):1-17. IF 3.092

3.092 25

99. Ullah S, Aslamkhan M, Ali A, Idrees M. Causes of deafness in the Punjab region of Pakistan and the role of consanguinity. Public Health, 2017; 145: 93-95. IF 1.952

1.952 02

100. Iqbal K, Hashmi AS, Idrees M, Shahzad S, Fatima Z. Polymerase Chain Reaction-Based Detection Method Is Suitable

1.432 0

17


for Dengue Infection During Epidemics. Viral Immunology. December 2017, 30(10): 734-736. https://doi.org/10.1089/vim.2017. 0057. IF 1.432

101. Manzoor F, Amin I, Shahid M, Afzal S, Ramzan H, Samiullah TR, Idrees M. Aedes aegypti is the Major Vector for Transmission of Dengue Virus in Lahore, Pakistan. Pakistan Journal of Zoology. vol. 49(2), pp 1-3, 2017. IF 0.79

0.79 01

102. Almas I, Afzal S, Idrees M, Ashraf MU, Amin I, Shahid M. Role of circulatory microRNAs in the pathogenesis of hepatitis C virus. VirusDiseases, Nov 2017; 28(1): DOI. 10.1007/s13337-017-0407-3. IF 0.364

0.364 07

103. Nawaz R, Zahid S, Idrees M, Rafique S, Shahid M, Ahad A, Amin I, Almas I, Afzal S. HCV-induced regulatory alterations of IL-1β, IL-6, TNF-α, and IFN-ϒ operative, leading liver en-route to non-alcoholic steatohepatitis. Inflamm Res. 2017 Jun;66(6): 477-486. doi: 10.1007/s00011-017-1029-3. Epub 2017 Mar 11.. IF 2.557

2.557 08

104. Wahid B, Ali A, Rafique S, Idrees M. Global expansion of Chikungunya Virus: mapping the 64 year history. International Journal of Infectious Diseases. 2017; Volume 58, Pages 69–76. IF 2.229

2.229 123

105. Nazir N, Jan MR, Ali A, Asif M, Idrees M, Nisar M, Zahoor M, El-Salam NM. Prevalence of hepatitis-C virus genotypes and potential transmission risks in Malakand Khyber Pakhtunkhwa, Pakistan. Virology Journal. (2017) 14:160. DOI 10.1186/s12985-017-0829-y. IF 2.465

2.759 04

106. Wahid B, Usman S, Ali A, Idrees M. Therapeutic Strategies of Clustered Regularly Interspaced Palindromic Repeats-Cas Systems for Different Viral Infections. Viral Immunology. August 2017. DOI: 10.1089/vim.2017.0055. IF 1.432

1.432 03

107. Munir S, Mahmood N, Shahid S, Idrees M. Molecular detection of Isoniazid, Rifampin and Ethambutol resistance to M. tuberculosis and M. bovis in multidrug resistant Tuberculosis (MDR-TB) patients in Pakistan. Microb Pathog. 2017 Sep;110:262-274. doi: 10.1016/j.micpath.2017.07.005. IF 2.960

2.960 02

108. Wahid B, Saleem K, Ali A, Idrees M. Rising relapse rate in hepatitis C virus type 3a-infected patients against sofosbuvir and ribavirin combination therapy: A Pakistani experience. European Journal of Gastroenterology and Hepatology. 2017 Aug; 29(8):979-980. doi: 10.1097/MEG.0000000000000895.. IF 1.968

1.968 08

109. Ali M, Khalil TA, Tanveer F, Ovais M, Yousaf MZ, Idrees M, Shinwari ZK. Thirty years of HIV in Pakistan; A systematic review of prevalence and current scenario. Future Virology. 2017; Volume 12 No. 10. https://doi.org/10.2217/fvl-2017-0009. IF 1.121

1.121 04

110. Wahid B, Ali A, Rafique S, Idrees M. Current status of 2.960 25

18


therapeutic and vaccine approaches against Zika virus. · European Journal of Internal Medicine. 2017 Oct;44:12-18. doi: 10.1016/j.ejim.2017.08.001. Epub 2017 Aug 7. IF 2.960

111. Shahid M, Amin I, Afzal S, Idrees M. Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan. Pakistan journal of Zoology 49(3):1119-1122 · May 2017. DOI: 10.17582/journal.pjz/2017.49.3.sc4. IF 0.79

0.79 0

112. Wahid B, Ali A, Rafique S, Idrees M. New Insights into the Epigenetics of Hepatocellular Carcinoma. BioMed Research International; 2017:1609575. doi: 10.1155/2017/1609575. Epub 2017 Mar 19. IF 2.476

2.476 48

113. Javeed S, Almas M…..Idrees M. Nrf2: A master regulator of cellular defense mechanism and a novel therapeutic factor. Pakistan Journal of Biotechnology. Vol. 14 (1) 121-126 (2017)

0

114. Ali A, Wahid, B, Rafique S, Idrees M, Advances in research on Zika Virus. Asian Pacific Journal of Tropical Medicine. 2017; 10(4): 321–331. IF 1.08

1.08 28

115. Khalil TA, Ali M, Tanveer F, Ovais M, Idrees M, Shinwari ZK, Hollenbeck JE. Emerging Viral Infections in Pakistan: Issues, Concerns, and Future Prospects. Health Security, Volume 15, Number 3, 2017, DOI: 10.1089/hs.2016.0072. IF 1.91

1.91 0

116. Khubaib, B, Idrees M, Fatima Z, Akram M, Afzal S, Amin I, Shahid M, Wasim M. Evaluation of three techniques for detection of IL28B SNP: A prognostic tool for HCV treatment outcome. Journal of Digestive Diseases. 2017; 18; 404–409. IF 1.07

1.07 02


Wahid B, Ali A, Waqar M, Idrees M. An increase in expression of SOCS1 gene in with increase in HCV viral load" Journal of Interferon and Cytokine Research. 2018 Mar; 38 (3):122-128. doi: 10.1089/jir.2017.0129. IF 2.377

2.377

0

118. Wahid B, Ali A, Waqar M, Idrees M. An updated systematic review of Zika virus-linked complications. Asian Pacific Journal of Tropical Medicine. Jan 2018; 11(1):1. DOI.10.4103/1995-7645.223527. IF 1.772

1.772 01

119. Wahid B, Ali A, Idrees M. Role of altered immune cells in liver diseases: a review" Gastroenterology Hepatology. 2018; 41(6): 377---388. doi: 10.1016/j.gastrohep.2018.01.014. IF 1.126

1.126 04

120. Wahid B, Saleem K, Waqar M, Wasim M, Sajjad, Idrees M. Successful treatment of HCV/TB co-infected patient with sofosbuvir and daclatasvir: a case report. Future Virology, Apr,2018;13(5): 10.2217/fvl-2017-0141. IF 1.121

1.121 02

121. Munir M, Rafique S, Ali A, Idrees M, Kang SM. Calculation of Wiener Indices of Thiazolides: The Potent Inhibitors of Hepatitis B Virus and Hepatitis C Virus Replication. Hepatitis Monthly.

1.677 0

19


2018 April; 18(4):e67709. IF 1.677 122. Wahid B, Saleem K, Waqar M, Wasim M, Sajjad, Idrees M.

Poor response to direct-acting antiviral therapy in HCV infected elderly population: a real-life-cohort study based on GenExpert Technology. Future Virology, 2018, 13(7):. IF 1.121

1.121 03

123. Basit Jabbar Outi Salo-Ahen, Ali A, Rafique S, Idrees M (2018). Antigenic Peptide Prediction From E6 and E7 Oncoproteins of HPV Types 16 and 18 for Therapeutic Vaccine Design Using Immunoinformatics and MD Simulation Analysis. Frontier in Immunology, 9: 3000. https://doi.org/10.3389/fimmu.2018. 03000.IF 5.511

5.511 04

124. Mirza MU, Vanmeert M, Ali A, Froeyen M, Idrees M (2018). Perspectives towards antiviral drug discovery against Ebola virus. Journal of Medical Virology, 2018 Nov 15. doi: 10.1002/jmv.25357. IF 2.049

2.049 06

125. Wahid B, Waqar M, Ali A, Iman K, Idrees M (2018). Recent trends in molecular epidemiology of Hepatitis C virus in Mardan, KPK Pakistan. Infection Genetics and Evolution, 66 (2018) 66–71. IF 2.611

2.611 08

126. Saleem S, Ali A, Khubaib B, Akram M, Fatima Z, Idrees M. Genetic diversity of Hepatitis C Virus in Pakistan using Next Generation Sequencing. Journal of Clinical Virology 2018 Nov;108: 26-31. doi: 10.1016/j.jcv.2018.09.001; IF 3.020

3.020 0

127. Ashraf MU, Imran K, Idrees M, Qureshi SA (2018). Evolution of efficacious pangenotypic hepatitis C virus therapies. Medicinal Research Review, 2018:1-43. doi: 10.1002/med.21554. IF 9.791

9.791 02

128. Wahid B, Saleem K, Waqar M, Wasim M, Sajjad, Idrees M. Poor glycemic control and abrupt onset of diabetes in HCV patients receiving direct acting antiviral drugs: Case series. Future Virology, 13 July, 2018, 13(8): 525-528. IF 1.121

1.121 02

129. Saleem K, Wahid B, Ali A, Rafiq S, Naz Z, Usman S, Idrees M (2018). Unexpected Response Profiles Seen in Hepatitis C Virus-Infected Patients Treated with Sofosbuvir Plus Ribavirin: Five Case Reports. Viral Immunology 2018 Jul/Aug; 31(6):480-483. doi: 10.1089/vim.2017.0199. IF. 1.432

1.432 01

130. Wahid B, Ali M, Rafique S, Waqar M, Wasim M, Idrees M. An overview of cancer immunotherapeutic Strategies. Immunotherapy. 2018 Aug;10 (11):999-1010. doi: 10.2217/imt-2018-0002. IF 3.461

3.461 04

131. Wahid B, Saleem K, Rasool N, Rafique S, Ali A, Waqar M, Idrees M. Tattooing trend: major cause of HCV transmission among youngsters. Infectious Diseases. 2018 Nov - Dec; 50(11-12):871-873. doi: 10.1080/23744235.2018.1518586. Epub 2018 Oct 14. IF 2.191

2.191 04

132. Wahid B, Ali A, Rafique S, Idrees M (2018). Zika virus: Is Pakistan next? Asian Pacific Journal of Tropical Medicine. 11(7):451-452. IF 1.772

1.772 0

20


133. Aftab M, Amin I, Idrees M, Ali A, Rafique S, Naz S (2018). Molecular Epidemiology of Hepatitis Delta and Hepatitis B Viruses Circulating In Two Major Provinces (East and North-West) of Pakistan. Infection Genetics & Evolution. 64 (2018) 65–69. IF 2.611

2.611 03

134. Naz Z, Wahid B, Usman S, Saleem K, Rafique S, Ali A, Idrees M. (2018). Expression of SOCS1 and SOCS3 genes in interferon treated and direct acting antiviral drugs treated hepatitis c patients. Journal of Interferon & Cytokine Research. 38(6):255-260. IF 2.377

2.377 06

135. Wahid B, Rafique S, Ali A, Waqar M, Nabi G, Wasim M, Idrees M. (2018). Biomarkers for diagnosis of pre-eclampsia and endometriosis. Biomarkers in Medicine, 2018 Oct;12(10):1161-1173. doi: 10.2217/bmm-2018-0058. Epub 2018 Sep 7. IF 2.346

2.346 0

136. Wahid B, Wasim M, Waqar M, Sajjad, Wahid K, Idrees M (2018) Explosive rise in viral infections through meat-sellers. Jundishapur Journal of Microbiology, 2018 June; 11(6): e68803. doi: 10.5812/jjm.68803. IF 1.03

1.03 0

137. Ali A, Ahmad H, Haider MGA, Idrees M, Ali I. Complete genome amplification and sequencing of DENV-2 Pakistani isolates: analysis reveals molecular evaluation of DENV-2. Future Virology, 2018; 13(12): 10.2217/fvl-2018-0149. IF 1.121

1.121 0

138. Maryam M, Idrees M. Study of promoter hypomethylation profiles of RAS oncogenes in hepatocellular carcinoma derived from hepatitis C virus genotype 3a in Pakistani population. Journal of Medical Virology. 2018 Sep;90(9): 1516-1523. doi: 10.1002/jmv.25221. Epub 2018 Jun 11. IF 2.049

2.049


Shams F, Rafique S, Zahid S, Munir M, Idrees M, Ilyas M, Husnain T. Advances in the role of HCV nonstructural protein 5a (NS5A) of 3a genotype in inducing insulin resistance by possible phosphorylation of AKT/PKB. Nature Scientific Reports. | (2019) 9:6150 | https://doi.org/10.1038/s41598-019-42602-2. IF 5.228

5.228

03

140. Mirza MU, Vanmeert M, Froeyen M, Ali A, Rafique S, Idrees M. In silico structural elucidation of RNA-dependent RNA polymerase towards the identification of potential Crimean-Congo Hemorrhagic Fever Virus inhibitors. Nature Scientific Reports. | (2019) 9:6809 | https://doi.org/10.1038/s41598-019-43129-2. IF 5.228

5.228 0

141. Shahid M, Idrees M, Butt AM, Raza SM, Amin I, Rasul A, Afzal S, Zahid S, Nawaz R. Hepatitis C and G virus coinfection in Punjab, Pakistan: incidence and its correlation analysis with clinical data. European Journal of Gastroenterology Hepatology. 2019 Mar;31(3):389-392. doi: 10.1097/MEG.0000000000001302. IF 2.198

2.198 02

21


142. Khan R, Khan A, Ali A, Idrees M (2019). The interplay between viruses and TRIM family proteins. Rev Med Virol. 2019 Mar; 29(2): e2028. IF 5.05

5.05 01

143. Ali A, Fatima Z, Wahid B, Rafique S, Idrees M. Cosmopolitan A1 lineage of dengue virus serotype 2is circulating in Pakistan: A study from 2017 dengue viral outbreak. Journal of Medical Virology. 2019; 91: 1909-1917. IF 2.049

2.049 02

144. Wahid B, Ali A, Idrees M. Prevalence of thyroid stimulating hormone dysfunction among sofosbuvir-treated HCV-infected patients: A real-world clinical experience. Journal of Medical Virology, 2019 Mar; 91(3):514-517. doi: 10.1002/jmv.25319. Oct 17. IF 2.049

2.049 02

145. Wahid B, Altaf S, Naeem N, Ilyas N, Idrees M. Scoping Review of Crimean-Congo Hemorrhagic Fever (CCHF) Literature and Implications of Future Research. Journal of the College of Physicians and Surgeons Pakistan 2019, Vol. 29 (6): 566-573. doi: 10.29271/jcpsp.2019.06.563 IF 0.407

0.407 04

146. Aftab, M, Naz S, Rafique S, Ali, A, Idrees, M (2019). Characterization of Hepatitis Delta Virus among pregnant women of Pakistan. Viral Immunology VOL. 32, NO. 8. https://doi.org/10.1089/vim.2019.0048 IF 1.417

1.417 0

147. Iqbal T, Rashid U, Idrees M, Afroz A, Kamili S, Purdy MA. A novel avian isolate of hepatitis E virus from Pakistan. Virology Journal 21 Nov 2019, volume 16, Article No: 142.

2.759 -

148. Amin I, Vajeeha A, Younas S, Afzal S, Shahid M, Nawaz R, Khan MU, Idrees M. HSV-1 Infection: Role of Viral Proteins and Cellular Receptors. Critical ReviewsTM in Eukaryotic Gene Expression 2019, 29(5): 461–469. IF 1.841

1.841 0

149. Shahid M, Amin I, Afzal S, Fatima Z, Idrees M. Comparative Analysis of Immunological and Genomic Outcomes of Dengue Virus Outbreak in Pakistan. Pakistan Journal of Zoology. vol. 51(5), pp 1971-1974, 2019. IF 0.79

0.79 0

150. Khan MA, Khan SA, Hamayun M, Ali M, Idrees M. Sequence variability of HCV 3a isolates based on core gene in patients from Lahore. Pakistan. Future Virology. (17 Dec 2019) 14(10), 641–653. IF 1.121

1.121 0

151. Naveed M, Ali A, Seikh N, Rafique S, Idrees M. Expression of TRIM22 mRNA in chronic hepatitis C patients treated with direct-acting antiviral drugs. APMIS, Journal of Pathology, Microbiology and Immunology, 2019; DOI 10.1111/apm.13024.

2.406 0

152. Iqbal T, Rashid U, Shahzad N, Afroz A, Malik MF, Idrees M. Molecular Detection of Hepatitis E Virus in Layer Chickens: A Possible Public Health Risk in Pakistan. 2019; Pakistan Journal of Zoology doi.org/10.17582/JOURNAL.PJZ.

0.79 0

153. Amin I, Younas S, Afzal S, Shahid M, Idrees M. HSV-1 and Host antiviral immune responses. Viral Immunology, 2019; 32(10): IF 1.417

1.417 0

22


154. Mirza, AU, Idrees M. Evolution of efficacious pangenotypic hepatitis C virus therapies. Medicinal Research Reviews, 2019; 39(3): 1091-1136. DOI: 10.1002/MED.21554. IF 9.7

9.7

155. Mirza MU, Vanmeert, M, Ali A, Iman K, Froeyen M, Idrees M. Perspectives towards antiviral drug discovery against Ebola virus. Journal of Medical Virology, 2019; 91(12): 2029-2048. IF 2.049

2.049

156. Bajwa MA, Idrees M, Devrajani T, Butt AK. Correlation and significance of dup A, ice A and hom B genes of Helicobacter pylori and other confounding factors in Pakistan Helicobacter pyloriisolates with gastroduodenal diseases. Indo American Journal of Pharmaceutical Research, 2019. 9(3): 2135-2142.


Naveed M, Ali M, Sheikh N, Rafique S, Idrees M. Expression of TRIM22 mRNA in chronic hepatitis C patients treated with direct-acting antiviral drugs. APMIS 2020-2019 Dec 20. doi: 10.1111/apm.13024. [Epub ahead of print] IF 2.67

2.67

0

158. Hassan A, Saeed A, Afzal S, Shahid M, Amin I, Idrees M. Applications and hazards associated with carbon nanotubes in biomedical sciences. Inorganic and Nano-metal Chemistry https://doi.org/10.1080/24701556.2020.1724151 IF 0.685

0.685 0

159. Shahid M, Idrees M, Amin I, Rasool A, Afzal S. Blood based gene expression profile of oxidative stress and antioxidant genes for identifying surrogate markers of liver tissue injury in chronic Hepatitis C patients. Archives of Virology 2020; 165:809–822. IF 2.5

2.5 0

160. Afzal M, Ali, A. Sheikh N, Rafique S, Idrees M, Peripheral Expression of CXCL10 Gene in Chronic Hepatitis C Patients treated with Sofosbuvir, Daclatasvir and Ribavirin. 2020; Journal of Interferon and Cytokine Research.2020; 40(6): IF 2.377

2.377 0

161. Shaheen M, Afzal S, Khubaib B, Amin I, Shahid M, Idrees M, Association of IL28B polymorphism with clearance of Hepatitis C Virus: A mini review. Critical Reviews in Eukaryotic Gene Expression. 2020,

1.84 0

162. Afzal S, Almas I, Imtiaz H, Shaheen MA, Daud M, Saghir A, Amin I, Shahid M, Idrees M. Drug resistance in Hepatitis C Virus: Future prospects and strategies to combat it. Critical Reviews in Eukaryotic Gene Expression. 2020; (Accepted) IF 1.84

1.84 0

163. Ali S, Afzal S, Yousaf MZ, Amin I, Shahid M, Idrees M. Paradoxical Role of Dengue Virus Envelope Protein Domain III Antibodies in Dengue Virus Infection. Critical Reviews in Eukaryotic Gene Expression. 2020; (Accepted) IF 1.84

1.84

164. Zahid K, Sana Shakoor S, Sajid HA, Afzal S, Ali L, Amin I,

Shahid M, Idrees M. Advancements in developing effective and preventive Dengue Vaccine. Journal: Future Virology (Accepted)

0.73

23


IF 0.73 165. Wahid B, Shami K, Idrees M. Comparing the risk of

hypothyroidism in HCV patients treated with different DAA drugs combination (sofosbuvir + interferon + ribavirin and sofosbuvir + daclatasvir + ribavirin). Journal of Medical Virology, 2020; 25931. DOI: 10.1002/jmv.25931. IF 2.373

2.373

166. Ayma Aftab, Muhammad Idrees, Samia Afzal. Identification and expression studies of DNA methylation profiles of promoter of p53 and rb genes during HCC infection. Ciência e Técnica Vitivinícola (Accepted for Publication) IF 0.85

0.85

167. Khan R, Ali A, Sheikh N, Rafique S, Idrees M (2020). Quantitative expression profiling of TRIM14 mRNA in chronic Hepatitis C Patients. Journal of Medical Virology. Accepted. IF 2.02.

2.02

168. Rafiq S, Zahid S, Ali A, Sheikh N, Rafique S, Idrees M (2020). Genome wide methylation profiling of HCV pathogenesis to develop diabetes and diabetic complications. Journal of Viral Hepatitis. Accepted. IF 3.75

3.75

169. Rafiq S, Idrees M (2020). Novel Coronavirus: A review from its origin to therapeutic strategies. APMIS-2020-124/R1 RESUBMISSION - (6346) Accepted article. IF 2.406

170. Bajwa MA, Idrees M, Maheshwari PK. Association of dupA, iceA, homB Genes of Helicobacter pylori with Gastritis, Peptic Ulcer Disease and Gastric Cancer. Journal of Pharmaceutical Research International. 2(25): 1-6, 2020; Article no. JPRI.60935.

Publications in National Scientific Journal with Impact Factor and/or HEC recognized

171. Idrees M*, Bhatti, A. H., Khan, S. U. and Riazuddin, S., 1998, Drug resistance in M. tuberculosis isolated from a group of patients referred to MRC Research Centre Mayo hospital Lahore, Pakistan. Pakistan Journal of Zoology 1998; 30(4): 335-339. IF 0.79

0.79 0

172. Idrees M*, Khan, S and Riazuddin. S. 2004. Common Genotypes of Hepatitis B Virus Present in Pakistan. JCPSP Journal of College of Physicians and Surgeons Pakistan. 14 (6): 344-7). IF 0.407

0.407 58

173. Idrees M*, Bhatti, A. H., Khan, S. U. Riazuddin, S., Majeed, A., Waheed, K and Hussain, G. Primary and acquired drug resistance in male and female TB patients in Lahore, Pakistan. In: Proceedings of 18th Pakistan Congress of Zoology, April 20-22. Pakistan Journal of Zoology 1998; 18: 123-129. IF 0.79

0.79 0

174. Waqar, A. B., Khan, S. and Idrees M*. Novelty in GB Virus C/ Hepatitis G Virus and its Controversy. JAMC 2002; 14 (3): 31-35.

0

175. Idrees M*., 2001. Detection of Six Serotypes of HCV in anti-HCV Positive Patients and rate of ALT/AST abnormalities. P. J. Microbiol, 1(2): 61-65.

0

24


176. Idrees M*, 2001, Common genotypes of hepatitis C virus present in Pakistan. Pak. J. Med. Res. 40(2): 46-49.

13

177. Idrees M*, Jan, A. H and Ahmad, H. 2001, Failure of Zooprophylaxis: cattle ownership increase rather than reduce the prevalence of malaria in district dir, NWFP of Pakistan. The Sciences, 1(2): 52-54.

06

178. Idrees M*, Comparison of two typing systems for genotyping of hepatitis C virus isolates. JCPSP Journal of College of Physicians and Surgeons Pakistan. 2000; 11(11): 22-25. IF 0.407

0.407 09

179. Idrees, M*, Bhatti,A.H.,Khan,S.U.and Riazuddin,S. Evaluation of PCR-SSCP: a rapid method for detection of rifampicin-resistant mutants in M. tuberculosis. JCPSP Journal of College of Physicians and Surgeons Pakistan. 2000; 10(5): 10-13. IF 0.407

0.407 0

180. Idrees, M., Bhatti,A.H., Khan, S.U. and Riazuddin, S. Study of primary initial and acquired drug resistance in M. tuberculosis to anti-TB drugs in Lahore, JCPSP Journal of College of Physicians and Surgeons Pakistan. 1998; 9(6): 257-260. IF 0.407

0.407 0

181. Idrees M*, Bhatti, A. H., Khan, S. U. Riazuddin, S., Majeed, A., Waheed, K and Hussain, G. Mycobacterium tuberculosis susceptibility testing against first line anti-TB drugs. Pakistan Journal of Biological Sciences. 1998; 2(2): (360-362).

05

182. Iqbal, S., Idrees M, Shabbir, I., Khan, M. M., Riazuddin, S. TB case management by doctors: Pakistan Journal of Medical Research. 2001; 40(2): 64-68. ISSN No. 0030-984.

0

183. Wahid B, Naeem N, Idrees M (2019). Current status of research on crimean-congo hemorrhagic fever: a scoping review. JCPSP Journal of College of Physicians and Surgeons Pakistan in press.

0.407 0

184. Sabar, M. F., Akram, M., Awan, F. I., Ghani, M. U., Shahid, M., Iqbal, Z., Kousar, S. Idrees, M. (2018) Awareness of Asthma Genetics in Pakistan: A Review with Some Recommendations. Advancements in Life Sciences. 6(1): 1-10.

0

185. Jahangir GZ, Naz S, Idrees M, Saleem MZ. Rapid RNA Extraction from Eucalyptus tree and its down processing for cloning of dehydrin genes. Adv. Life Sci. 2018; 5(4): 185-191.

0

186. Shahzad M, Shafique M, Hussain M, Shan MA, Perveen R, Ziaurehman, Idrees M. Successful DNA Profiling for Identification of burnt Families from their bones using AmpFℓSTR Identifiler® Plus Kit (2016). Adv. Life Sci. 3(2); 59-62

02

187. Shah AR, Idrees M, Hussain A. Hepatitis G virus associated aplastic anemia: a recent case from Pakistan. Virology Journal. 8 (1), 30.

2.759 21

Publications in International Scientific Journal with No Impact Factor

25


188. Ali L, Idrees M, Ali M, Hussain A, Rehman IU, Ali A, Iqbal AS, Kamel ES. Inhibitory effect of kaolin minerals compound against hepatitis C virus in Huh-7 cell lines. BMC Research Notes. 2014, 7: 247. doi:10.1186/1756-0500-7-247. IF 1.6

03

189. MS Alam, SU Khan, S Ayaz, N ul Akbar, MA Khan, I Ahmad, M Idrees M. Molecular detection of Giardia lamblia and Cryptosporidium parvum in different water sources of district bannu, Khyber Pakhtunkhwa, Province of Pakistan. Microbiology Research Journal International, 2014, 80-88.

07

190. Waqar, M, AU Khan, A Ali, M Wasim, Idrees M, Z Ismail, AA Noor, N Akbar. Prevalence and molecular determination of Hepatitis C infection in Khyber Pakhtunkhwa, Pakistan. 2014, Archives of clinical infectious diseases 9 (3), 1-5.

04

191. Rehman HU, Wasim M, Waqar Khan AU, Idrees M, Ismail Z, Ali A, Faisal, Seroprevalence of HBV infection and viral loads in outpatients attending a district hospital located in Mardan, Pakistan. International Journal of Biosciences. 03/2014; 4(5): 28-34. DOI:10.12692/ijb/4.5.28-34. IF 0.553

0

192. Wasim M., Biland B, Idrees M, et al., Assessment of Risk Factors and Clinical Presentations in a Liver Cirrhotic State-Pakistan. World Applied Sciences Journal. 2014; 32 (7): 1252-1257. IF 0.47

0

193. Waqar M, Rehman HU, Khan SU, Noor AA, Ali A, Wasim M, Idrees M, Ismail Z, Niaz Z, Akbar NU, Tariq M. Frequency Distribution of Hepatitis C Virus Genotypes in District Karachi, Pakistan. Journal of Gastroenterology and Hepatology Research. 2014 April 21 3(4): 1035-1038.

0

194. Amin I, Idrees M, Awan Z, Shahid M, Afzal S, Hussain A. PCR could be a method of choice for identification of both pulmonary and extra-pulmonary tuberculosis. BMC Research Notes. 2011, 4:332. IF 1.6

40

195. Afzal S, Idrees M, Almas I, Shahid M. Study of inhibition of interferon inducible genes by dephosphorylation of E2 envelope gene of HCV genotype 1a. Conference Paper · January 2017. DOI: 10.1109/IBCAST.2017.7868053. Conference: 2017 14th International Bhurban Conference on Applied Sciences and Technology (IBCAST)

0

196. Awan Z, Idrees M*, Rafique S, Rehman IU, Akbar H, Butt S, Manzoor S, Khan LA. Hepatitis B virus YMDD-motif mutations with emergence of lamivudine-resistant mutants: a threat to recovery. Gastroenterol Hepatol FBB, 2010; 3(3):108-114.

09

197. Khan AA, Inam S, Idrees M, Gul K and Akbar H. Effect of automobile workshop on the health status of automechanics in NWFP, Pakistan. A. J. Environ Sci Technol 2010; 4 (4): 192-200.

17

198. Jafri SSI, Ilyas M, Idrees M*. Swine flu: A threat to human health. Biotech Mol Biol. 2010; 5(3): 46-50.

07

199. Akbar H, Khan M, Khan AA, Khan MA, Idrees M, Comparitive 0

26


efficacy of Doxycycline and Flumequine. J Veteran Med Anim Health, 2009; 1(2): 017-022.

200. Akbar H, Idrees, M*, Manzoor S, Rehman IU. Hepatitis C virus infection: A review of the current and future aspects and concerns in Pakistan. J Gen Mol Virol 2009; 1(2): 012-018.

44

201. Jaferi SSI, Ilyas M, Idrees M*. AIDS Crisis in Pakistan. J Gen Mol Virol 2009; 1 (3): 033-034.

0

202. Javeed S, Almas M, Shahid M, Amin I, Idrees M. Review Nrf2: A master regulator of cellular defense mechanism and a novel therapeutic factor. Pak J Biotech 2017; 14(1):121-126.

0

203. Amin I, Naz S, Shahid M, Ashraf U, Rasheed A, Rasul A, Nawaz R, Zahid S, Almas I, Zahid K, Manzoor F, Idrees M. Inhibitory Effects of Thiazolide Compounds against Hepatitis C virus: in vitro Study. International Journal of Virology and Molecular Biology 2016, 5(2): 27-33.

0

204. Malik K*, Sabir K, Shabir N, Nisar N, Basit MH, Idrees M, Ambreen HS, Sumrin A, Sadia H. Controversies: Stem Cell Research. Bull. Env. Pharmacol. Life Sci., Vol 5 [5] April 2016: 77-83. IF 0.982.

0

205. Perveen R1, * Ahmad Ali Shahid, Muhammad Shafique, Idrees M, Tayyab Husnain;Genetic Signatures of Y chromosome STRsin Sindhi Population of Pakistan (proceedings of YHRD-Haploid Markers 2016 update on DNA variation Berlin, Germany, May 20-21, 2016. p-A24).

0

TOTAL Impact Factor & Citations 411.826 4,808

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Citations Indices of Dr. Idrees’s Articles (Through Web of Science)

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Publication metrics

These metrics are calculated from the publications you have imported. It may take up to an hour after importing publications for these metrics to be updated.

Publications in Sum of times Average citations Average citations Web of Science Cited H-index per item per year

195 2,913 26 10.3 150.4

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Citation indices & Citations to Dr. Idrees’s articles (Through Google Scholar) (Updated on 01-07-2020)

Table: Citation indices All Since 2015

Citations 4,808 2,689

h-index 31 26

i10-index 80 67

100

200

300

400

500

600

Figure: Citations to Prof. Dr. Muhammad Idrees’s articles

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Citation Indices (Copied First two pages only)

Prof. Dr. Muhammad Idrees Vice Chancellor, Hazara University Molecular Virology, Hepatitis, HCV, HBV, Molecular epidemiology Verified email at cemb.edu.pk

Articles 1–177

Title Cited by Year

Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission M Idrees, S Riazuddin BMC infectious diseases 8 (1), 69

288 2008

Transition metal complexes as potential therapeutic agents S Rafique, M Idrees, A Nasim, H Akbar, A Athar Biotechnology and Molecular Biology Reviews 5 (2), 38-45

285 2010

Oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer R Faridi, A Zahra, K Khan, M Idrees Virology Journal 8 (1), 269

246 2011

Hepatitis C treatment: current and future perspectives S Munir, S Saleem, M Idrees, A Tariq, S Butt, B Rauff, A Hussain, S Badar, ... Virology journal 7 (1), 296

161 2010

A study of best positive predictors for sustained virologic response to interferon alpha plus ribavirin therapy in naive chronic hepatitis C patients M Idrees, S Riazuddin BMC gastroenterology 9 (1), 5

145 2009

Hepatitis associated aplastic anemia: a review B Rauff, M Idrees, SAR Shah, S Butt, AM Butt, L Ali, A Hussain, M Ali Virology Journal 8 (1), 87

138 2011

Hepatitis B virus in Pakistan: a systematic review of prevalence, risk factors, awareness status and genotypes M Ali, M Idrees, L Ali, A Hussain, IU Rehman, S Saleem, S Afzal, S Butt Virology journal 8 (1), 102

137 2011

High prevalence of hepatitis C virus infection in the largest province of Pakistan M Idrees, A Lal, M Naseem, M Khalid Journal of digestive diseases 9 (2), 95-103

121 2008

Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience M Idrees, S Rafique, I Rehman, H Akbar, MZ Yousaf, S Butt, Z Awan, ... World Journal of Gastroenterology: WJG 15 (40), 5080

112 2009

Common genotypes of hepatitis B virus. M Idrees, S Khan, S Riazuddin

88 2004

Citation indices All Since 2015

Citations 4,808 2,689

h-index 32 26

i10-index 80 67

31


Title Cited by Year

Journal of the College of Physicians and Surgeons--Pakistan: JCPSP 14 (6 …

Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan Z Fatima, M Idrees, MA Bajwa, Z Tahir, O Ullah, MQ Zia, A Hussain, ... BMC microbiology 11 (1), 200

87 2011

Development of an improved genotyping assay for the detection of hepatitis C virus genotypes and subtypes in Pakistan M Idrees Journal of virological methods 150 (1-2), 50-56

76 2008

Avian influenza A (H9N2): computational molecular analysis and phylogenetic characterization of viral surface proteins isolated between 1997 and 2009 from the hu... AM Butt, S Siddique, M Idrees, Y Tong Virology journal 7 (1), 319

69 2010

Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan A Ali, H Ahmed, M Idrees Virology Journal 7 (1), 203

67 2010

Determination of HCV genotypes and viral loads in chronic HCV infected patients of Hazara Pakistan A Ali, M Nisar, H Ahmad, N Saif, M Idrees, MA Bajwa Virology journal 8 (1), 466

60 2011

The changing epidemiology pattern and frequency distribution of hepatitis C virus in Pakistan S Butt, M Idrees, H Akbar, I ur Rehman, Z Awan, S Afzal, A Hussain, ... Infection, Genetics and Evolution 10 (5), 595-600

55 2010

Hepatitis E virus-associated aplastic anaemia: the first case of its kind SAR Shah, A Lal, M Idrees, A Hussain, C Jeet, FA Malik, Z Iqbal, ... Journal of Clinical Virology 54 (1), 96-97

53 2012

H-Factor (H-Index) (Through Google Scholar; Updated on 1-10-2020)

Table: Citation indices All Since 2015 Citations 4049 2678

H-index 32 26 i10-index 80 67

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RESEARCH:- Scientific Works (As a researcher) Dr. Muhammad Idrees, currently serving CEMB, has a distinguished career as a research scientist.

M. Sc. (Zoology) RESEARCH WORK

He started research career in 1995 with M.Sc. (Zoology) thesis work on "The Dilemma of Zoo-Prophylaxes with special reference to prevalence of Malaria in District Dir, NWFP Pakistan" under the supervision of Prof. Dr. Abdul Hameed Jan (Zoology Department Peshawar University). He conducted parasite prevalence survey in order to examine the possibility that domestic cattle kept in house courtyards might protect occupants against malaria through zooprophylaxis. However, his finding showed that Malaria parasite rate was higher significantly (p<0.01) among families which kept cattle than among those which did not keep it.

M. Phil. (Molecular Biology) RESEARCH WORK

Dr. Idrees started to work for M. Phil. thesis in 1998 on Multiple Drug Resistance and its responsible Genes/mutations in Mycobacterium tuberculosis under the direct supervision of Prof. Dr. Fazal Majid Khan (CEMB University of the Punjab). Initially susceptibility testing was done with eight commonly used anti-TB drugs. These studies revealed a growing concern of increasing prevalence of drug resistance in M. tuberculosis. Next, he developed and evaluated a rapid and simple technique `PCR-SSCP` for the detection of Rifampicin resistance in Mycobacterium tuberculosis associated with mutations in the gene that is responsible for the synthesis of RNA polymerase enzyme. The information, that all the mutations in the RNA polymerase gene (rpo B) are clustered within a region of 23 amino acids, was used for the development of Polymerase Chain Reaction-Single Strand Conformation Polymorphism that detected Rifampicin resistant mutations in culture isolates. This was a next step towards the development of a rapid detection method for multidrug- tuberculosis cases.

Ph. D. (Molecular Biology) RESEARCH WORK In year 2000, he started to work for Ph.D. thesis on ‘Sequence Variability studies in Hepatitis C Virus genome and its implication on treatment response and disease outcome’ under the supervision of Prof. Dr. Sheikh Riazuddin. First Objective: For the first time in Pakistan, we were able to sequence the 5’Untranslated Region (5’UTR) region of HCV-RNA isolated from HCV positive patients from different parts of Pakistan. During the course of this study, HCV RNA PCR positive serum samples collected from different parts of the country were used for sequence analysis of 5’UTR of HCV. Four primers were designed based on highly conserved 5’UTR of HCV genome for the amplification and sequencing of 5’NCR. The sequence from nt: -35 to -319 (285 nt) was taken for analysis. Pakistani isolates sequenced in the present study were aligned with the representative number of sequences for each major genotype and subtype selected from the GenBank database. The results of the phylogenetic analysis of the sequenced samples showed for the first time that genotype 3 and type 1 are the predominant genotypes circulating in Pakistan, with an overall prevalence of 62% and 12%, respectively. Then we were able to (second objective) investigate and isolate novel HCV isolates from Pakistan. On the basis of phylogenetic analysis, the Pakistani isolates were classified as follows: 38% (n = 38) were type 3a, 21% (n = 21) were type 3b, 3% (n = 3) were type 3c, 7% (n = 7) were type 1a, 5% (n = 5) were type 1b/1c, 5% (n = 5) were type 2a/2c, 3% (n = 3) were type 2b, 3% (n = 3) were type 4, and 14% (n =

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14) were novel genotypes. One isolate, PP10, still remained nontypeable. The overall nucleotide similarity among different Pakistani isolates was 92.50% + 0.50%. The percent nucleotide identity (PNI) was 98.11% + 0.50% within Pakistani type 1 sequences, 98.10% + 0.60% for type 2 sequences, 97.97% + 0.50% for type 3 sequences, and 99.80% + 0.20% for type 4 sequences. The PNI between different genotypes was 93.90% + 0.20% for type 1 and type 3, 94.80% + 0.12% for type 1 and type 4, and 94.40% + 0.22% for type 3 and type 4. There was a stretch of hypervariable region from nt: 166 to 86 in the 5’NCR that had motifs for different genotypes. Pakistani isolates showed 7.5% + 0.50% nucleotide variability in this region. The comparatively conserved stretch from nt 241 to 165 showed only 3.30% + 1.06% variation. It was not possible to differentiate between type 1b and 1c isolates further into different subtypes. Both types clustered together. Similarly, in the case of 2a and 2c, no differentiation was possible in the tree. In the case of the type 3 isolates, there was a clear clustering of isolates into subtypes 3a, 3b and 3c. This study showed that type 3 and type 1 seem to be the predominant genotypes circulating in different parts of Pakistan. More or less equal proportions of type1 and type3 isolates were seen in Bal. The third objective of his study was to establish a simple, rapid and reliable genotyping system for well-characterized Pakistani HCV isolates and to determine the suitability of this assay for the routine determination of HCV genotypes. He established a new Molecular-based genotyping system for the specific detection of HCV genotypes 1a, 1b, 1c, 2a, 2b, 2c, 3a, 3b, 3c, 4a-h, 5a and 6a during the course of this study. Total 15 primers were designed for this new genotyping system. Both the outer sense and anti-sense primers and inner sense primer were designed on the basis of the conserved nature of these sequences. Genotype-specific primers were designed on the basis of 116 HCV isolates. The antisense primers were designed on the basis of the conserved nature of those sequences within a genotype and their poor homology with the sequences derived from other HCV genotypes. Extensive experiments were performed to optimize the genotyping PCR. Serum samples having known genotypes were used as positive controls to validate the developed assays and to generate PCR band patterns. Band patterns generated from the clinical serum samples from HCV patients were compared to the patterns produced from these control samples. In addition, the type-specific bands were sequenced from the test patients and control clinical samples to further validate the nested type-specific PCR test results. Sensitivity and specificity of the system was determined by analyzing 260 samples simultaneously by this HCV genotyping system, Ohno’s genotyping system and commercially available serotyping kit. The system showed 79.2% concordance with Ohno’s system and 65.38% with Serotyping system. Samples with discordant results were sequenced and their genotypes were determined by molecular evolutionary analysis. In all the discordant samples the assigned genotype of the present system was correct. The fourth objective of his present study was to establish a genotyping system for mixed HCV infections as to date the true prevalence of HCV mixed-genotype infections has not been established mainly because currently available methods are not suitable for the detection of mixed genotypes in a viral population. A new genotyping method, primer-specific PCR followed by polyacrylamide gel electrophoresis (TSPCR-PAGE), which is more reliable than other genotyping assays was developed for the detection of HCV mixed-genotype infections. A genotype present at levels as low as 8.3% in a defined mix of HCV genotypes was detected, showing a 20-fold increase in sensitivity over that of direct DNA sequencing. A total of 50 HCV isolates from patients received multiple blood transfusions were genotyped and analyzed for a comparative study of the accuracy between the present system and two

34


other genotyping methods. The results showed that viruses in approximately 42% of the samples from this group determined to be infected with mixed genotypes by TSPCR-PAGE. Serotyping assay and restriction fragment length polymorphism analysis performed poorly, being able to identify only 18% and 10% of mixed-genotype infections, respectively. The data lead him to conclude that HCV mixed-genotype infections are more common than previously estimated and that TSPCR-PAGE may be the method of choice when detection of genotypes present at low levels in mixed-genotype infections is required due to its higher level of sensitivity. The fifth specific objective of his study was to find out the common genotypes of HCV present in well-characterized Pakistani HCV isolates. Total 3351 serum samples were tested by type-specific genotyping assay. All the tested serum samples were HCV-RNA positive by PCR. Out of the total 3351 tested serum samples, type-specific PCR fragments were observed in 3150 (94.00%) serum samples. The distribution of genotypes of the 3150 typable samples as determined by this assay, was as follows: 280 (8.35%) patients were infected with HCV genotype 1a, 101 (3.01%) patient were infected with genotype 1b, 5 (0.15%) with 1c, 252 (7.52%) patient were infected with subtype 2a, 27 (0.80%) were infected with subtype 2b, 3 (0.09%) were with type 2c, 1664 (49.05%) patients were infected with subtype 3a, 592 (17.66%) were infected with genotype 3b, 25 (0.75%) were with 3c, 50 (2%) patient were infected with genotype 4, 6 (0.18%) were infected with subtype 5a, 4(0.12%) were infected with subtype 6a and 161 (4.80%) patient was infected with mixed infection. Two hundred and one (6.00%) serum samples were found undetermined by the present genotyping system. The aims of the 6th objective of his study were to determine the rate of sustained virological response (SVR) and the factors that are associated with SVR in chronic hepatitis C patients treated with interferon alpha and ribavirin combination therapy. Four hundred consecutive patients were prospectively evaluated and treated with combination of Interferon Alfa 2 (a or b) three million units subcutaneously three injection weekly and ribavirin 800-1200 mg orally daily for 24 weeks or 48 weeks and followed for another 6 months. End of the treatment response, sustained viral response and side effects were noted. Of four hundred patients, 394 completed the treatment. Over 67% responded at the end of treatment and 16% relapsed. Out of treated, 48.55% males and 57.62% females had sustained viral response with a total combined sustained viral response rate of 51.56%. Patients with cirrhosis had no sustained viral response. Thirty percent patients took longer than three months to show HCV RNA negativity. Side effects were usual and tolerable and only 1.5% discontinued the treatment. Non-responders were mostly males above age 50 years. In conclusion, twenty-four weeks combination treatment with Interferon alpha 2a or 2b and ribavirin has given 51.56% sustained viral response in patients and treatment was well tolerated. The seventh objective of his research was to assess the association between chronic hepatitis C

infection with different genotypes and hepatocellular carcinoma (HCC) in a population of patients presenting to a various hospitals. Eighty-three cases of histologically confirmed liver cancer due to HCV presenting to the various hospital of Pakistan were genotyped. HCV genotype 3a was predominant (34 of 83 patients). In the HCC patient population, broader distributions of genotypes were present (genotype 1a: 8; genotype 1b: 2; genotype 3a: 34; genotype 3b: 13; mixed genotypes 3a & 3b: 10; 1a & 3a: 8; and 1a & 3b: 6). Two of the cases were untypable as no type-specific bands were obtained for them. He observed a strong association between chronic HCV infection and hepatocarcinogenesis in the Pakistani population.

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d. ADMINISTRATIVE/POSTDOCTORAL RESEARCH EXPERIENCE IN THE FIELD OF HIGHER EDUCATION:

Dr. Idrees has sufficient administrative and Postdoctoral research experience in the field of Higher Education.

I). Administrative Experience in the Field of Higher Education: Dr. Idrees have a demonstrated track record of leadership in higher education institutions/universities, and career profile with diverse training and experience. He has proven and well-established credential and experiences of about 20 years as Vice Chancellor, Director, Incharge Commercial Diagnostic Lab (Public sector), Group Leader of a Research Group etc. 1. Vice Chancellor, Hazara University Mansehra, Khyber Pakhtunkhwa- From July 28,

2016- July 28, 2019: As a Vice Chancellor of Hazara University for about three years, his experience of academic planning, academic program review & curriculum development; supervision of budget preparation for the academic and academic-related programs of Hazara University, allocating and reallocating funds and resources to and from these programs; provision of leadership in creating and maintaining academic standards and policies; chair the Syndicate, Academic Council of Hazara University, the General Board of the Faculties and the Finance Committee of the Council; Provide leadership, academic and administrative to the whole University; Represent the University externally, both within the country and overseas; Secure a financial base sufficient to allow the delivery of the University’s mission, aims and objectives. Further, having post doctorate from USA and FRCPath from United Kingdom can provide the required academic leadership and administrative experience to be Vice Chancellor of any university of Khyber Pakhtunkhwa. He is/was:

2. Director, Center for Applied Molecular Biology, University of the Punjab Lahore-From July 1, 2015-July 27, 2016 and was:

Chairman/Convener, Purchase Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015-July 27, 2016.

Chairman/Convener, Technical Evaluation Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015- July 27, 2016.

Convener/Chairman, Board of Studies (BOS), CAMB, University of the Punjab Lahore. From 2015- July 27, 2016.

Member, Board of Faculty (BOF), University of the Punjab Lahore. From 2015-ongoing.

Member Board of Studies (BOS) Department of Genetics, Hazara University Garden Campus Mansehra, KPK-From 2011- July 27, 2016.

Member, Academic Council University of the Punjab Lahore. From 22-8-2015- July 27, 2016.

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3. Incharge Disease Diagnostic Facility, CAMB, Ministry of Science & Technology Government of Pakistan- From 2001- 2009. Establishment of Commercial Molecular Diagnostic Facility for the detection of infectious

& genetic diseases at Centre for Applied Molecular Biology (CAMB), MoST and provided general public with reliable, accurate, rapid and more economical diagnostic service for most prevalent infectious and genetic diseases.

Generated a Source of Income for the Center: On daily basis this Lab is receiving more than 250 samples from different tertiary care hospital of the country that is the largest number of samples in the country. Present income of the Lab is about 5.0 million per month (60 million/year). In year 2013, an amount of 350 million was deposited to Government of Pakistan as generated revenue through MoST.

Developed Molecular Diagnostic Kits: As a part of these endeavors, to develop indigenous technologies, Dr. Idrees’s Group have developed Molecular Diagnostic Kits for the qualitative detection of hepatitis B virus, hepatitis C virus, M. tuberculosis and HCV Genotyping at 1/10th the cost of the same kits manufactured by other companies in USA.

Helped Prime Minister Program for Prevention & Control of Hepatitis: Due to vital importance of viral testing, as gesture and the noble consent, his Group had provided Molecular Diagnostic Facilities to the patients booked for hepatitis B&C infection treatment in the 104 sentinel sites in the country under the PM’s Program for Prevention & Control of Hepatitis. and has performed more than 50,000 HCV quantitative/HCV genotyping and HBV quantitative tests free of charges to the poor patients. The market value of these tests is more than 113.00 million.

4. Group Leader, Infectious Diseases & Molecular Virology Group CEMB, University of the Punjab- From 2000- continue (both Basic & Applied research).

5. Member of a team of four Scientist designated by CDC, USA to visit Pakistan (2006-07) to meet with several public health officials from the Ministry of Heath and the National Institute of Health to discuss various collaborative public health programs and activities that will support the Field Epidemiology & Laboratory Training Program (FELTP).






11. Member Biosafety & Bioresource Committee University of the Punjab to review the research proposals of the faculty members and Ph. D scholars before submission to funding agencies. From 22-9-2015-ongoing.

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II). Postdoctoral Research Experience in the Field of Higher Education: After obtaining Ph. D. in Molecular Virology and in keeping with his bright academic record, Dr. Idrees subsequently worked for his post-doctoral research from September 2006 to March 2007 at in the best research laboratories in the world – Division of Viral Hepatitis (DVH), Coordinating Centers for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta GA USA under the supervision of Dr. Yury Khudyakov (Deputy Brach Chief) and Dr. Saleem Kamili (Branch Chief). The DVH, in collaboration with U.S. and global partners, provides the scientific and programmatic foundation and leadership for the prevention and control of viral hepatitis infections and their manifestations. At DVH, he was a part of various domestic and global activities, including exciting outbreak investigations; surveillance programs; analytic, cost- effectiveness, vaccination, and behavioral studies; and the prevention and control of blood-borne, enteric, sexual, and chronic diseases.

After his return to Pakistan, Dr. Idrees prepared and submitted a joint research proposal with Branch Chief, Dr. Yury Khudyakov of CDC to USA State Department for funding. The project proposed to develop a series of streamlined techniques for rapid evaluation of HCV genome heterogeneity and study of HCV heterogeneity in different epidemiological and clinical settings. All the major objectives of these studies were achieved such as:

(1) Developed a new streamlined PCR based approach and serological assay for the identification of HCV genotypes and mixed genotype infections;

(2) Conducted population-based analysis of HCV genotype distribution in Pakistan and rate of viral clearance in patients infected with different HCV genotypes or mixed HCV genotypes;

(3) Developed novel approaches to the identification of HCV transmission between hosts using MS profiles of HCV quasispecies and serological profiles of antibody binding to a set of HCV sequence variants of antigens derived from several different HCV trains;

(4) Conducted analysis of MS quasispecies profiles and serological antibody binding patterns on a large set of HCV chronic cases and patients with HCC;

(5) Conducted sequence analysis of full HCV genome sequences from patients with chronic hepatitis C and HCC in order to identify viral genetic parameters associated with these outcomes of HCV infection;

(6) Built computational models predictive of these outcomes. FRCPath: Fellowship of Royal College of Pathologists by Published Works

As recognition of tremendous contribution in the field of Molecular Biology/Virology, The Royal College of Pathologists London-UK awarded him with Fellowship of the College (FRCPath) on September 18, 2015 (Publications-based). Dr. Idrees’s virology work relates to six main themes which consist of studies on:

A. Establishment of assays/methods to study viruses; B. Virus genome characterization, variability, genotypes/serotypes analysis; C. Viral and host genes/proteins and their role in disease outcome and vaccine development; D. Virus prevalence, molecular epidemiology and outbreak investigation; E. Role of various viral & host factors in treatment response rates; and F. Virus association with other abnormal conditions/diseases.

In addition, Dr. Idrees has participated in other studies which include diseases of tuberculosis, typhoid, malaria, anemia, carcinomas etc. Theme-wise detail of published research work is given:

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A. Establishment and/or evaluation of different assays/methods to study viruses

Pub No.

Publication information

1 Afzal S, Idrees M*, Hussain M. De Novo modeling of Envelope 2 protein of HCV isolated from Pakistani patient and epitopes prediction for vaccine development. Journal of Translational Medicine 2014; 12:115. doi:10.1186/1479-5876-12-115.

2 Rafique S, Idrees M*, Ali A, Sahibzada KI and Iqbal M. Generation and using of HCV infectious pseudo typed particles for studying the role of CD81 & SRB1 receptors in HCV infection. Molecular Biology Reports 2014 Jun 19; 41(6):3813-3819. Epub 2014 Feb 19.

3 Rafique S, Idrees M*, Ali A and Iqbal M. Studies on the role of neutralizing antibodies against envelope genes in resolving HCV pseudo-particles infectivity. Mol Biology Reports 2014 Jun 19; 41(6):3945-3950

4 Idrees M*, Amreek Lal, Fayyaz Ahmed Malik, Abrar Hussain, Irshad ur Rehman,Haji Akbar, Sadia Butt, Muhammad Ali, Liaqat Ali, Fayyaz Ahmed Malik. Occult hepatitis C virus infection and associated predictive factors: the Pakistan experience. Infection, Genetics and Evolution 2011 Mar; 11(2): 442-445.

5 Idrees M*. Rehman IU, Manzoor S. Akbar H. Butt S, Afzal S, Yousaf MZ, Hussain A Evaluation of three different HCV typing methods for detection of mixed-genotype infections. J. Digestive Diseases. 2011 June; 12(3): 199-203.

6 Yousaf MZ, Idrees M*, Saleem MZ, Ali M, Expression of core antigen of HCV genotype 3a and its evaluation as screening agent for HCV infection in Pakistan. Virology Journal. 2011 July 26; 8: 364.

7 Rafique S, Nasim A, Idrees M*. Riazuddin S. Stable expression of envelope glycoproteins of HCV genotype 3a in Huh-7 cell-lines. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Aug-Oct; 1(2): 199-213.

8 Idrees M*, Development of a New HCV Genotyping Assay and its Suitability for the Detection of Common Genotypes in Hepatitis C Patients in Pakistan. J. Virol Methods. 2008 June; 150(1-2): 50-56.

9 Ali A, Nisar M, Idrees M*, Rafique S, Iqbal M Expression of Hepatitis C Virus Core and E2 antigenic recombinant proteins and their use for development of diagnostic assays. International Journal of Infectious Diseases 2015 March 18; e84-e89.

B. Studies on Virus Genome characterization, variability and Genotypes/ Serotypes analysis

Pub No.


10 Ur Rehman I, Vaughan G, Purdy MA, Xia GL, Forbi JC, Rossi LM, Butt S, Idrees M, Khudyakov YE. Genetic History of Hepatitis C Virus in Pakistan. Infection, Genetics and Evolution 2014 Oct;27:318-324.

11 Ali M. Rehman IU, Idrees M*. Emergence of genetically variant Hepatitis C virus population in response to increased antiviral drug pressure, Pakistan. Virus Genes, 2014 Jun; 48(3):543-9.

12 Butt, FA, Amin I, Idrees M*, Iqbal M. Hepatitis delta virus genotype-1 alone co-circulates with hepatitis B virus genotypes A and D in Pakistan. Eur. J. Gastroenterology Hepatology. 2014 Mar; 26(3):319-324.

13 Waqar M, Khan AU, RehmanHU, Idrees M*, Wasim M, Ali A, Niaz Z, Ismail Z, Rehman MU, Tariq M, Shah M, Murtaza BN. Determination of Hepatitis C Virus Genotypes Circulating in Different Districts of Punjab Pakistan. European Journal of Gastroenterology and

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Hepatology. 2014 Jan; 26(1):59-64. 14 Hussain A, Idrees M*. First complete genome of HCV-1a form Pakistani isolate and its

Phylogenetic analysis with complete genome of rest of world. Virology J. 2013, 10:211. doi:10.1186/1743-422X-10-211.

15 Butt S, Idrees M*, Amin I, Younas S, Afzal S, Akbar H, Rehman IU. Change in hepatitis C virus clades: A cross-sectional study of chronic HCV in Pakistan from 2000-2010. Eur J Clinical Microbiology and Infectious Diseases. 2011 May; 30(5): 669-672. doi: 10.1007/s10096-010-1138-1. Epub 2011 Jan 6

16 Fatima Z, Idrees M*, Bajwa MA, Tahir Z, Ullah O, Zia MQ, Hussain A, Akram M, Khubaib B, Afzal S, Munir S, Saleem S, Rauff B, Badar S, Naudhani M, Butt S, Aftab M, Ali L, Ali M. Serotype and genotype analysis of Dengue Virus by Sequencing followed by Phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan. BMC Microbiology 2011 Sep; 11: 200.

17 Ali A, Nisar M, Ahmad A, Saif N, Idrees M*, Bajwa MA. Determination of HCV genotypes and viral loads in chronic HCV infected patients of Hazara Pakistan Virology Journal 2011 Oct; 8:466.

18 Ullah I, Idrees M*, Ahmad H, Ghafoor SU, Ali M, Ali L, Ahmad A. Hepatitis C virus genotypes circulating in district Swat of Khyber Pakhtoonkhaw, Pakistan. Virology Journal. 2011 Jan; 8:16.

19 Butt S, Idrees M*, Akbar H, ur Rehman I, Awan Z, Rehman IU, Hussain A, Afzal S, Shahid M, Manzoor S, Rafique S. The changing Epidemiology pattern and Frequency Distribution of hepatitis C virus in Pakistan. Infection, Genetics and Evolution 2010 Jul; 10(5): 595-600. Epub 2010 May 9.

20 Idrees M*, Butt S, Awan Z, Aftab M, Khubaib B, Rehman IU, Akram M, Mamzoor S, Akbar H, Rafique S, Riazuddin S. Nucleotide identity and variability among different Pakistani hepatitis C virus isolates. Virology Journal. 2009 Aug 24; 6: 130.

21 Idrees M*, Riazuddin S. Frequency Distribution of Hepatitis C Virus Genotypes in Different Geographical Regions of Pakistan and their Possible Routes of Transmission, BMC Infectious Dis 2008 May 23; 8: 69.

22 Idrees M*, Khan, S and Riazuddin. S. 2004. Common Genotypes of Hepatitis B Virus Present in Pakistan. Journal College of Physician and Surgeon Pakistan. 14 (6): 344-7).

C. Studies on viral and/or host genes/proteins and their role in disease outcome and Vaccine

development using Bioinformatics tools

Pub No


23 Amat-Ur-Rasool H, Saghir A, Idrees M*. Computational Prediction and Analysis of Envelop Glycoprotein Epitopes of DENV-2 and DENV-3 Pakistani isolates: A First Step towards Dengue Vaccine Development. PLoS One. 2015, Mar 16; 10(3): e0119854.

24 Butt A, Idrees M, Tong Y. Identification and expression profiling of microRNAs in hepatitis C patients by using next generation sequencing. Journal of Viral Hepatitis 2014 Oct 14; Vol 21 (S2), 33–33.

25 Butt AM, Feng D, Nasrullah I, Tahir S, Idrees M, Yigang Tong Y, Lu J. Computational identification of interplay between phosphory-lation and O-β-glycosylation of human occludin as potential mechanism to impair hepatitis C virus entry. Infection Genetics Evolution. 2012 Aug 10; 12(6): 1235-1245.

26 Butt AM, Khan IB, Hussain M, Idrees M, Lu J, Tong Y. Role of post translational

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modifications and novel crosstalk between phosphorylation and O-beta-GlcNAc modifications in human claudin-1, -3 and -4. Molecular Biology Reports. 2012 Feb; 39(2): 1359-1369.

27 Butt AM, Feng D, Idrees M, Tong Y, Lu J. Computational identification and modeling of crosstalk between phosphorylation, O-beta-glycosylation and methylation of FoxO3 and implications for cancer therapeutics. International Journal of Molecular Sciences 2012; 13(3), 2918-2938.

28 Rafique S, Idrees M*, Ilyas M, Hussain A, Ali M, Ali L, Butt S, Afzal S, Rehman IU, Saleem S. Positional effect of phosphorylation sites 266 and 267 in the cytoplasmic domain of the E2 protein of hepatitis C virus 3a genotype and Interferon: Resistance through approach of Sequence Alignment. Virology Journal 2011 May 5; 8: 204. doi:10.1186/1743-422X-8-204

29 Afzal S, Idrees M, Ali M, Ilyas M, Akram M, Butt S, Salem S, Hussain A, Rehman IU, Ali L, Shahid M. Envelope 2 protein phosphorylation sites S75 & 277 of hepatitis C virus genotype 1a and interferon resistance: A sequence alignment approach. Virology J. 2011, Feb 15; 8:71.

30 Shehzadi A, Rehman SU, Idrees M*. Promiscuous prediction and conservancy analysis of CTL binding epitopes of HCV3a viral proteome from Punjab Pak:an In Silico Approach. Virology J 2011 Feb 8; 8: 55.

31 Butt AM, Siddique S, Idrees M, Tong Y. Avian influenza A (H9N2): computational molecular analysis and phylogenetic characterization of viral surface proteins isolated between 1997 and 2009 from the human population. Virology Journal 2010 Nov 15; 7: 319.

D. Virus Prevalence, Molecular Epidemiology and Outbreak Investigations

Pub No


32 Ali A, Rehman HU, Nisar M, Rafique S, Ali S, Hussain A, Nausheen, Idrees M*, Sabri S, Zada H, Hussain S. Seroepidemiology of dengue fever in Khyber Pakhtunkhawa-Pakistan International Journal of Infectious Diseases 2013 Jul; 17 (7): e518-e523. doi:10.1016/j.ijid.2013.01.007

33 Idrees M*, Hussain W, Rehman HU, Tayyab GN. Afzal S, Fatima Z, Akram M, Raza SM, Ali L, Hussain A, Amin I, Shahid M, Khubaib B, Saleem S, Nasir B, Tariq A, Wasim M, Waqar M. Dengue Virus Serotype 2 (DEN-2): the Causative Agent of 2011-Dengue Epidemic in Pakistan. American J Biomed Sciences. 2012 Aug; 4(4), 307-315. doi: 10.5099/aj120400307

34 Butt, S., Idrees M*, Rehman IR, Akbar H, Shahid M, Afzal S, Younas S, Amin I. High Prevalence of dual HCV infection. Emerging Infectious Diseases. 2011 Aug; 17(8): 1565-1567.

35 Khan AU, Waqar M, Akram M, Zaib M, Wasim M, Ahmad S, Niaz Z, Ali S, Ali H, Idrees M*. Bajwa MA. True prevalence of Twin HDV-HBV infection in Pakistan: A Molecular approach. Virology Journal 2011 Sep 4; 8: 420. doi:10.1186/1743-422X-8-420

36 Khan F, Akbar H, Idrees M, Khan H, Shahzad K, Kayani MA. The prevalence of HBV infection in the cohort of IDPs of war against terrorism in Malakand Division of Northern Pakistan BMC Infectious Diseases 2011, Jun 20; 11:176. doi:10.1186/1471-2334-11-176

37 Iqbal T, Idrees M*. Ali L, Hussan A, Ali M, Butt S, Yousaf MZ, Sabar MF. Isolation and characterization of two new Hepatitis E Virus Genotype 1 strains from two Mini-outbreaks in Lahore, Pakistan. Virology Journal 2011 Mar 4; 8: 94. doi:10.1186/1743-422X-8-94

38 Ali A, Ahmed H*, Ali I, Khan S, Zaidi G, Idrees M*, Prevalence of active Hepatitis C Virus infection in District Mansehra Pakistan'. Virology Journal. 2010 Nov 22; 7: 334.

39 Zaidi G, Idrees M*, Malik FA, Amin I, Shahid M, Younas S, Hussain R. Awan Z, Tariq A,

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Parveen K. Prevalence of Hepatitis Delta Virus in Hepatitis B surface antigen positive patients circulating in the largest province of Pakistan. Virology Journal. 2010 Oct 26; 7: 283.

40 Ali A, Ahmed H, Idrees M*, Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan. Virology J. 2010 Aug 26; 7: 203.

41 Awan Z, Idrees M*, Amin I, Butt S, Afzal S, Akbar H, Rehman IU, Younas S, and Shahid M. Lal A, Saleem S, Rauff B. Pattern and Molecular Epidemiology of Hepatitis B virus genotypes circulating in Pakistan. Infection, Genetics and Evolution. 2010 Aug 17; 10(6): 1242-1246

42 Idrees M*, Lal A, Naseem M, Khalid M. High Prevalence of Hepatitis C Virus Infection in the Largest Province of Pakistan. Journal of Digestive Diseases. 2008 May; 9(2): 95-103.

43 Ali A, Nisar M, Idrees M*, Ahmad H. Hussain A, Rafique S, Sabri S, Rehman HU, Ali L, Wazir S, Khan T. Prevalence of HBV infection in suspected population of conflict-affected area of war against terrorism in North Waziristan FATA Pakistan. Infection, Genetics and Evolution. 2012 Dec; 12(8); 1865-1869.

44 Riaz M Idrees M*, Kanwal H, Kabir F. An overview of Triple infection with Hepatitis B, C and D viruses. Virology Journal 2011, Jul; 8: 368.

45 Ali M, Idrees M*. Ali L, Hussain A, Rehman IU Saleem S, Afzal S, Butt S. Hepatitis B virus in Pakistan: A systemic review of prevalence, risk factors, awareness status and genotypes. Virology Journal 2011, Mar 6; 8; 102. doi:10.1186/1743-422X-8-102

E. Studies on Anti-viral therapy: role of various viral & host factors in treatment response rates Pub No.


46 Ullah S, Rehman HU, Idrees M*. Mutations in the NS5a gene are associated with response to interferon + ribavirin combination therapy in patients with chronic hepatitis C virus 3a infection. European Journal of Gastroenterology & Hepatology 2013 Oct; 25(10):1146-1151.

47 Akram M, Idrees M*, Hussain A, Afzal S, Ilyas M, Zafar S, Aftab M, Badar S, Khubaib B. Characterization of Hepatitis C Virus genotype 3a Hypervariable region 1 in patients achieved rapid virological response to alpha interferon and Ribavirin Combination therapy. Virology Journal 2011 May 23, 8(1): 253. doi: 10.1186/1743-422X-8-253.

48 Akram M, Idrees M*, Zafar S, Hussain A, Butt S. Afzal S, Rehman IU, Liaqat A, Saleem S, Ali M, Butt A. Effects of Host and virus related factors on Interferon-alpha + ribavirin and Pegylated-interferon+ribavirin treatment outcomes in Chronic Hepatitis C patients. Virology Journal 2011, May 17; 8: 234. doi: 10.1186/1743-422X-8-234.

49 Akbar H, Idrees M*, Butt S, Awan Z, Sabar MF, Rehaman Iu, Hussain A, Saleem S. High Baseline Interleukine-8 level is a Independent Risk Factor for the achievement of Sustained Virological Response in Chronic HCV Patients. Infection, Genetics and Evolution 2011 Aug; 11(6): 1301-1305.

50 Ali I, Khan S, Attaullah S, Khan SN, Khan J, Siraj S, Iqbal A, Swati ZA, Idrees M. Response to combination therapy of HCV 3a Infected Pakistani patients and the role of NS5A protein. Virology Journal 2011 May 25; 8: 258.

51 Afzal S, Idrees M*, Akram M, Awan Z. Khubaib B, Aftab M, Fatima Z, Badar S, Hussain A. Mutations in the E2-PePHD region of hepatitis C virus genotype-3a and correlation with response to interferon and ribavirin combination therapy in Pakistani patients Virology Journal. 2010 Dec 31; 7: 377.

52 Idrees, M*, Riazuddin S. A study of best positive predictors for sustained virologic response to interferon alpha plus ribavirin therapy in naive chronic hepatitis C patients. BMC Gastroenterol 2009 Jan 20; 9:5.

53 Khubaib B, Idrees M*, Afzal S, Wasim M. The genotype CC of IL-28B SNP rs12979860 is

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significantly associated with a sustained virological response in chronic HCV Pakistani patients. Journal of Digestive Diseases 2015 Feb 24; (In Press). doi: 10.1111/1751-2980.12238. [Epub ahead of print]

54 Ali L, Idrees M*. Ali M, Rehman IU, Hussain A, Afzal S, Butt S, Saleem S, Munir S, Badar S. An overview of treatment response rates to various anti-viral drugs in Pakistani Hepatitis B Virus infected patients. Virology Journal 2011 Jan 15; 8: 20. doi: 10.1186/1743-422X-8-20.

55 Munir S, Saleem S, Idrees M*. Tariq A, Butt S, Rauff B, Hussain A, Badar S, Fatima z, Ali M, Ali L, Akram M, Aftam M, Khubaib B, Awan Z. Hepatitis C Treatment: current and future perspectives. Virology Journal. 2010 Nov 1; 7: 296. doi:10.1186/1743-422X-7-296

F. Studies on Virus association with other abnormal conditions/Diseases

Pub No.


56 Sabri S, Idrees M*, Rafique S, Ali A, Iqbal M. Studies on the role of NS3 and NS5A non-structural genes of HCV 3a local isolates in apoptosis Intern J. Infectious. Diseases. 2014, Aug; 25: 38-44.

57 Shahid M, Idrees M*, Nasir B, Raja AJ, Raza SM, Amin I, Rasul A, Tayyab GU. Correlation of biochemical markers and HCV RNA titers with fibrosis stages and grades in chronic HCV-3a patients. European Journal of Gastroenterology and Hepatology. 2014 Jul; 26(7):788-794.

58 Shah AR, Lal A, Idrees M*, Hussain A, Jeet C, Malik FA, Iqbal Z, Rehman HU, Hepatitis E Virus Associated Aplastic Anemia: First case of its kind. J. Clinical Virology. 2012 May; 54(1): 96-97.

59 Shah SAR, Idrees M*, Hussain A. Hepatitis G associated aplastic anemia in Pakistan: A recent case from Pakistan. Virology Journal, 2011 Jan 21; 8: 30. doi:10.1186/1743-422X-8-30

60 Manzoor S, Idrees M*, Ashraf A, Mehmood A, Butt S, Fatima K, Akbar H, Rehman IU, Qadri Q. Identification of ionotrophic purinergic receptors in Huh-7 cells and their response towards structural proteins of HCV genotype 3a. Virology Journal 2011 Sep 8; 8: 431.

61 Idrees, M*, Rafique S, Rehman I, Butt S, Riazuddin S. The impact of hepatitis C virus genotype 3a infection on the development of hepatocellular carcinoma: Pakistan experience. World Journal of Gastroenterology 2009 Oct 28; 15(40): 5080-5085.

62 Badar S, Khubaib B, Idrees M*. Hussain A, Awan Z, Butt S, Afzal S, Akram M, Fatima Z, Aftab M, Saleem S, Munir S, Ali L, Rehman IU., Association of Hepatitis C Virus With Insulin Resistance: Evidences From Animal Studies and Clinical Studies. Hepatitis Monthly 2012 Jan; 12(1): 11-15.

63 Faridi R, Zahra A, Idrees M*, Khan K. Oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer. Virology Journal 2011 Jun 3; 8: 269.

64 Rauff B, Idrees M*. Shah AR, Butt S, Butt AM, Ali L, Hussain A, Rehman IU, Ali M. Hepatitis Associated Aplastic Anemia: A review. Virology Journal 2011 Feb 28; 8: 887.

A. First Them-Establishment of assays/methods to study viruses In first theme (Theme A), Dr. Idrees has authored 9 publications where Dr. Idrees is the first and/or senior/corresponding author in 7 (Publication # 1,2,3,4,5,8&9), sole author in one (Publication #8), corresponding author in 4 (Publication # 1, 2, 3 & 9) and contributing author in 2 (Publication # 6&7). These publications describes the establishment of various PCR based and cell culture based assays to study viruses. His publication #1 describes De Novo modeling, isolation & structural analysis of patient

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derived HCV-3a E2 glycoprotein sequences and B-cells and T-cells-specific epitopes prediction for vaccine development. The sequence, structural and epitope analysis has revealed a number of conserved epitopes in both 3a and 1a genotypes. These epitopes may not only help in diagnosing the pathogens but also may help in developing vaccine against HCV 3a and 1a genotypes. Presence of overlapping epitopes generates the hope that a small fragment of peptide in vaccine formulation can elicit broad immune response and may result in efficient clearing of HCV pathogen. Dr. Idrees has played a key role in development of infectious pseudo particles of local HCV3a-isolate and further checked HCVpp infectivity in Huh-7 cell lines. He found that a number of other liver-specific surface proteins along with CD81 and SRBI receptors are concurrently responsible for HCV infections (Publication #2). In addition, he studied the role of neutralizing antibodies against E1 and E2 genes in resolving HCVpp infectivity by HCV-3a Pakistani isolate. This was the first study on the role of neutralizing antibodies against envelope genes in resolving HCV-3a pseudo-particles infectivity is available from this region of the globe (Publication #3). Dr. Idrees’s group has developed a cell-culture based system (Publication #7) for screening of HCV anti envelope neutralizing antibodies in the serum of HCV patients during acute and chronic HCV infections. This system can be used for the screening of antiviral molecules that can block the entry of the virus into host cell. Dr. Idrees was the first in the country who attempted to determine the etiology of the elevated liver-enzyme levels by investigating occult HCV infection in these patients, utilizing a sensitive real-time PCR assays to detect HCV RNA in liver biopsy specimens from a cohort of patients who were negative for anti-HCV antibodies, negative serum HCV RNA and had abnormal LFTs of unknown origin (Publication #4). Dr. Idrees has developed a new highly sensitive, specific, reliable, reproducible and economic HCV genotyping assay for the specific detection of HCV genotypes 1a, 1b, 1c, 2a, 2b, 2c, 3a, 3b, 3c, 4a–h, 5a and 6a (Publication #8). This method is capable of genotyping reliably HCV RNA directly from clinical samples in routine diagnostic laboratory. His publication # 5 describes the evaluation and clinical applicability of three different genotyping (including his newly developed method) and serotyping assays for the true prevalence of HCV mixed-genotype infections. Their newly developed assay was able to detect mixed genotype at very low level and may be the method of choice when detection of genotypes present at low levels in mixed-genotype infections due to its higher level of sensitivity. Dr. Idrees et al. (Publication #6) has devised a screening assay for the first time in Pakistan ever, utilizing core antigen of HCV genotype 3a. Their developed screening assay is more sensitive, specific and reproducible than the commercially available screening assays in Pakistan. More recently, Dr. Idrees and his colleague have also developed highly sensitive, inexpensive and simple serological assays for the screening of HCV infection utilizing mixture of recombinant (Core & E2) antigens to detect the presence of HCV antibodies in serum specimens (Publication #9).

B. Virus genome characterization, variability, genotypes/serotypes analysis In subsequent (Theme-B) virus genome characterization, variability, genotypes/serotypes analysis, Dr. Idrees has co-authored 13 publications. This series of investigations aimed at identifying the evolutionary history, genetic heterogeneity, discoveries of novel mutations, full-length genome characterizations, distribution of different circulating viruses in different parts and ethnic groups of the country. Recently, Dr. Idrees has presented the evolutionary history of HCV genotype 3a in Pakistan for the first time by analyzing the genetic heterogeneity of HCV NS3 and NS5b subgenomic regions (Publication #10). This article resulted from his collaborating work in Dr. Yury’s laboratory at US-CDC, Atlanta GA. Their results show a remarkable genetic diversity among HCV variants in that locale, and

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substantiate the hypothesis that HCV genotype 3a originated in the Indian subcontinent. Phylogenetic analyses showed that Pakistani genotype 3a variants were as genetically diverse as global variants, with extensive intermixing. Bayesian estimates showed that the most recent ancestor for genotype 3a in Pakistan was last extant in 1896–1914 C.E. (range: 1851–1932). This genotype experienced a population expansion starting from 1905 to 1970 after which the effective population leveled. Death/birth models suggest that HCV 3a has reached saturating diversity with decreasing turnover rate and positive extinction. In addition, Dr. Idrees has discovered novel mutations such as D/N244E, K304R, N/K307G, Q/T329V, and A338V that were found associated with the emerging clades of HCV3a resistance to anti-viral therapy (Publication #11). His publication # 13 describes the frequency distribution and pattern of various HCV genotypes circulating in the different districts of Punjab in different age groups and sexes. Dr. Idrees further studied the change in HCV pattern of distinct clades over 11 years by genotyping more than 22’000 chronic HCV infected patients. A changing pattern in HCV clades was seen in this region as a significant decline was observed in the percentages of HCV clades 2, 4, 5 & 6 and increase in genotype 1 (Publication #15). In addition, he was able to discovered six new HCV genotypes such as 1c, 2c, 3c, 4, 5a and 6a for the first time from Pakistan. This changing pattern in HCV genotypes local Pakistani HCV infected patients may influence success in treating HCV infection in this country and may make the present situation difficult (Publication #19). His group was also involved in the genotypes/subtypes analysis of circulating HCV genotypes and their possible routes of transmission in other geographical regions including Hazara Division (Publication #17), Swat district of Khyber Pakhtoonkhawa (Publication #18) and rest of the whole country (Publication #21). Dr. Idrees’s group has reported the first patient derived hepatitis C virus (HCV) complete genome from Pakistan (Publication #14) as was not previously available from this region of the world The evolutionary divergence analysis for nucleotide and amino acid sequences, conducted by equal input model, suggested that evolutionary nucleotide and amino acid distances showed that the HCV Pakistani strain was genetically far from Denmark strain (0.29400 nt, 0.819646 aa) and near to German strain (0.06557 nt, 0.139449 aa), respectively. For the first time in Pakistan he had determined percent nucleotide identity and variability in HCV isolates prevalent in different geographical regions of Pakistan in year 2001 when few peoples were aware about HCV genome (Publication # 20). Regarding studies on HBV, Dr. Idrees has found out the frequency distribution of common genotypes HBV circulating in all the provinces and was the first virologist of the country who done such type of studies (Publication #22). Further, Dr. Idrees has carried out a study on genotyping of both HBV and HDV in chronically co-infected patients and studied specific interaction between different HBV and HDV genotypes (Publication #12). In addition, Dr. Idrees’s group have studied in detail three mini outbreaks of dengue virus infection that occurred in years 2007, 2008 & 2009 (Publication #16).

C. Viral and host genes/proteins and their role in disease outcome and vaccine development In studies on virus and/or host gens/proteins and their role in disease outcome/vaccine development using Bio-informatics tools (Theme-C), Dr. Idrees has co-authored 9 publications. In a more recent study published in high impact factor journal (Publication #23), Dr. Idrees and his coworkers used a number of bioinformatics tools to analyze various features of antigenic epitopes including antigenecity, surface accessibility, flexibility, residue solvent accessibility, spatial distribution and inter-molecular contacts for Dengue virus (DENV) E-protein. Their predicted epitopes are important candidates for developing vaccines. Dr. Idrees has played a key role in the prediction of linear and conformational B-

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cell epitopes of DENV envelope glycoprotein. His proposed continuous and discontinuous antigenic peptides can be valuable candidates for DENV diagnostic and therapeutic. The most important publication of this theme is the genome-wide identification and expression profiling of microRNAs in HCV patients by using next generation sequencing (NGS) techniques (Publication #24). Taken together, his report is the first report of NGS based miRNA profiling in HCV patients at varying levels of disease stages. I believe that the findings of this study will be helpful in understanding and expanding current knowledge on the role of miRNAs in HCV disease progression and therapeutic strategies. His publication #25 describes computational identification of interplay between phosphorylation and O-b-glycosylation of human occludin as potential mechanism to impair HCV entry. To my knowledge, this is the first study to report the O-b-GlcNAc potential of occludin and target sites of ERK (Ser8, Ser310, and Thr345), GSK-3 (Ser8, Ser341) and Cdk5 (Thr376). As targeting viral entry holds great therapeutic promise, therefore, his discovery of Yin Yang sites represents an attractive strategy to impair HCV entry. Similarly they were able to find out novel crosstalk between phosphorylation and O-beta-GlcNAc modifications in human claudin-1, -3 and -4 (Publication #26). In addition, a computational workflow and set of selection parameters modeling of crosstalk between Phosphorylation, O-β-glycosylation and Methylation of FoxO3 have been defined for the identification of target sites and crosstalk between different PTMs (Publication #27). Their findings may facilitate the study of therapeutic strategies targeting posttranslational events. Their findings may facilitate the production of anti-cancer drugs for different cancers including hepatocellular Carcinoma (HCC). His publication #28 describes a hybrid in-silico and wet laboratory approach of motif prediction, evolutionary and structural analysis. Dr Idrees has pointed out nucleotide variations in serine 266 and 267 of the HCV-3a E2 gene as a hopeful claimant for the serine phosphorylation that may assist to propose genotype precise therapy to avoid and resolve HCV infections. Using similar approach he has discovered novel nucleotide variations in serine 75 and 277 of the HCV-1a E2 gene as a promising candidate for the serine phosphorylation that may have a significant role in interferon resistance (Publication #29). Next, Dr. Idrees analyzed the entire HCV proteome of Pakistani origin, aimed to identify the viral epitopes and their conservancy in HCV genotypes 1, 2 and 3 of diverse origin using immunoinformatic tools (Publication #30). All the predicted epitopes have important implications in diagnostics as well as CTL-based rational vaccine design, effective for most population of the world and especially the Pakistani Population. Dr. Idrees also conducted an in silico analysis of H9N2 viruses and identified potential novel reassortments and zoonotic potential (Publication #31). His findings support the continuous evolution of avian H9N2 viruses towards human as host and are in favor of effective surveillance and better characterization studies to address this issue. Findings from the study support the genetic instability of influenza A (H9N2) viruses.

D. Virus prevalence, molecular epidemiology and outbreak investigation In theme-D, Dr. Idrees holds 14 publications on virus prevalence, molecular epidemiology and outbreak investigation. Recently, Dr. Idrees has determined the seroprevalence of dengue (IgM and IgG) antibodies and the disease symptoms in the urban as well as in rural areas population of Khyber Pakhtunkhawa, in different seasons. Dengue infection was most prominent in the post-monsoon season, in urban areas, and in young patients with a history of travel to an endemic locality (Publication #32). Dr. Idrees has also carried out a comprehensive study of the 2011 major outbreaks of dengue virus infection on molecular level in which more than 50,000 people were infected. Based

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on the results of his study, DEN-2 was the responsible genotype for that major dengue epidemic that started from the beginning of year 2011 and is continuing till now. The additional serotype detected was serotype 3 (Publication #33). Regarding work on HCV, Dr. Idrees has surveyed and estimated the prevalence and spectrum of active HCV infection in apparently healthy inhabitants in different age and gender groups of District Mansehra (Publication #38), Khyber Pakhtoonkhaw (Publication #40) and in the entire general population of Pakistan (publication #42). These were the first reports that showed the true picture of high seroprevalence of anti-HCV antibodies in a general and HCV RNA in apparently healthy population of Pakistan. Of highest impact factor (7.3327) is the publication in Emerging Infectious Diseases (2011), where Dr. Idrees is the major contributing and corresponding author (Manuscript #34). This publication describes retrospective analysis of genotyping data for paired serum samples from 22,125 HCV-infected patients to determine the prevalence of HCV mixed genotype infections during the past 11 years for all regions in Pakistan. He found a significantly elevated prevalence of HCV mixed genotype infections in Pakistan. Dr. Idrees also demonstrated the prevalence of HBV infection the various socio-economic, demographic and possible risk factors related to HBV infection among the conflict-affected peoples due to war against terrorism in Federal Administered Areas of Pakistan (Manusript #43). Dr. Idrees has further studied the pattern and molecular epidemiology of HBV genotypes circulating in Pakistan (Publication # 41). This is the first and sole study on the subject and represents all the geographical regions of the country. In addition, Dr. Idrees also demonstrated the prevalence of HBV infection in the cohort of internally displaced people (IDPs) of war against terrorism in of Northern of Pakistan (Publication #36). In addition, Dr. Idrees has found out the true prevalence of twin HBV-HDV in different geographical regions of Pakistan for the first time in the country (Publication #35). According to his research the HDV coinfection is common in Pakistan and its prevalence is higher significantly in the Province of Sindh and male six. Dr. Idrees’ group also determined the prevalence of HDV super-infection among HBsAg positive individuals in the highly populated province of Pakistan (Publication#39). Importantly, Dr. Idrees’ group isolated and characterized two novel hepatitis E virus genotype 1 strains from two mini-outbreaks in Lahore (Publication #37). Their study suggests that both the human HEV strains were closely related to the Sar-55 and is the main endemic HEV strain circulating in various areas of the country. In the last, Dr. Idrees has also published two review articles related to this specific theme. The first one (Publication #44) is a detailed overview on the epidemiology, pathogenesis, transmission, symptoms, diagnosis, replication, disease outcome, treatment and preventive measures of triple hepatitis B, C & delta infections. The second article (#45) is a systematic review on HBV prevalence, risk factors, awareness status and genotypes.

E. Role of various viral & host factors in treatment response rates In Studies on role of various viral and host factors on response rates during antiviral therapy, Dr. Idrees has published 10 articles (Theme-E). Recently, Dr. Idrees has discovered an association between HCV3a-NS5A gene mutations and response to interferon+ribavirin combination therapy in patients with chronic HCV (Publication #46). His findings as well as other studies support the hypothesis that HCV-3a resistance toward combination therapy is due to mutations in the ISDR region of the NS5A gene. His another study describes novel mutations in other parts of the viral genome coupled with mutations in the ISDR responsible for anti-viral resistance (Publication #50). In another study, Dr. Idrees has also characterized Hypervariable region-1 of HCV3a infected patients at baseline and

47


correlated antigenic variability with rapid virological responders (RVR), breakthrough and non-responders (Publication #47). Antigenic variability was more significant in RVR than in breakthrough responders. Before, Dr. Idrees has studied the effects of various host and virus related factors and analyzed rate of early and delayed response to antiviral treatment as well as rate of relapse response in chronic HCV patients following both standard IFN a/ribavirin and pegylated interferon treatment (Publication #48). Dr. Idrees has further investigated the relationship between baseline levels of IL-8 in serum, HCV infection, and response to IFN standard therapy (publication #49). His findings showed that high baseline interleukine-8 level is an independent risk factor for the achievement of sustained virological response in chronic HCV patients and the changes induced in the level of IL-8 by HCV infection has a predictive value for the net outcome of therapy. In addition, Dr. Idrees has correlated different mutations in the E2-PePHD region of HCV-3a with treatment response (Publication #51). Based on his results, he concluded that PePHD domain in local HCV-3a strains carries novel substitutions that tend to decrease the average hydrophilic activity of PePHD domain in RVR and increase the average hydrophilic activity in breakthrough responders. This leads HCV to persist by evading antiviral activity of interferon alpha. Dr. Idrees has determined the rate of sustained virological response (SVR) and various factors associated with response rates in chronic hepatitis C infected patients treated with interferon alpha and ribavirin combination therapy (Publication #52). His study showed that standard interferon therapy may be as useful as PEG-interferon plus ribavirin for SVR in Pakistan. Patients with RVR, low pre-treatment viral load, HCV genotypes 2 & 3, age < 40 years and ethnic group Pashtoon appear to have the highest probability of ETR and SVR. More recently Dr. Idrees has evaluated the association of genetic variation in interleukin 28B (IL28B) and viral influential factors with treatment outcome in chronic HCV patients (Publication #53). The CC genotype of SNP rs12979860 of IL28B was found a strong independent predictive factor for SVR in Chronic HCV infected patients. SNP rs12979860 CC genotype has statistically strong association with successful treatment outcome in chronic HCV infected patients. Publication # 55 is a review article containing recent advancement in current therapy and future perspectives of HCV treatment. Publication #54 contains an overview on treatment response rates to various anti-viral drugs in Pakistani HBV infected patients.

F. Virus association with other abnormal conditions/diseases Dr. Idrees’s accomplishments on virus association with other abnormal conditions/diseases include 9 publications (Theme-F). Dr. Idrees has recently studied the role of NS3 & NS5A non-structural genes of HCV-3a local isolates on apoptosis (Publication #56). Dr. Idrees has successfully established Huh 7 cells lines stably expressing NS3 and NS5A protein of HCV which were used to study the molecular mechanism underlying and studied their relationships with apoptotic protein p53. Dr. Idrees’s manuscript is the first report of its kind in Pakistan and may be useful for the development of new therapeutic strategies. The developed model system has the potential to be used for the screening of antiviral drugs. Dr. Idrees also correlated biochemical markers and HCV RNA titers with fibrosis stages and grades in chronic HCV-3a patients (Publication #57). Dr. Idrees has shown several viral and host markers that have significant correlation with liver injury in CHC patients. Their study suggests that various baseline viral and host factors can be indicative of ongoing liver damage. These noninvasive markers may substitute the necessity for liver biopsy in patients with cirrhosis and in whom the procedure has known limitations and complications. Another research was undertaken by

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Dr. Idrees to identify the presence of various isoforms of P2X receptors in human hepatoma cell (Huh-7) and to evaluate the response of identified isoforms to HCV structural proteins E1E2 (Publication #60). His findings demonstrated that each of these isoforms of P2X receptors respond differently to HCV structural proteins E1E2. This is one of the first studies which may open a new insight into the pathogenic mechanisms leading to HCV induced liver pathogenesis via Purinergic (P2X) receptor signaling. Furthermore, these findings also suggest that the most responsive isoform P2X4 receptors could be represented a novel and functionally important component of the purinergic signaling complex in HCV induced pathogenesis. Dr. Idrees has also assessed the association between chronic HCV infection and hepatocellular carcinoma (HCC) in Pakistan, and the genotype distribution among these HCC patients (Publication #61). He has found a strong association between chronic HCV infection and HCC in Pakistan, and between HCV-3a and HCC. During the last four years, Dr. Idrees has extensively studied hepatitis associated aplastic anemia (HAAA). He used various hematological, biochemical, immunological and virological markers for the detection of the causative agent of HAAA. Several viruses such as HCV, HBV, HEV and HGV were found associated with aplastic anemia. His discovered HEV-associated aplastic anaemia is the first case of its kind in the world (Publication #58). Similarly, Dr. Idrees has established the involvement of HGV in the development of aplastic anemia (Publication #59). Based on his research, Dr. Idrees has suggested that in patients presenting with pancytopenia after an episode of acute hepatitis, the definitive diagnosis should be considered and confirmed by RT-PCR and if possible, by bone marrow biopsy. Dr. Idrees has also published a review article containing a comprehensive literature search to understand the nature and association between HCV infection and insulin resistance (IR) in modulating the course of the insulin-signaling pathways, giving evidences from animal and clinical studies (Publication #62). His another review article (Publication #63) describes oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer. The basic purpose of this review article was to highlight the dire need of awareness of this deadly cancer as increase in risk of HPV infection has a tremendous role in women related sexual problems and the hazardous effect is in the form of cervical cancer which is the major cause of death among all the cancers in developing countries. The most important and last article of this theme is a review on hepatitis associated aplastic anemia (Publication #64) that is a well-documented and diverse variant of clinical syndrome of aplastic anemia, in which an acute attack of hepatitis leads to the marrow failure and pancytopenia that may be acute or chronic. In this review Dr. Idrees has described the reported cases of this disorder in the world, its relation with low socioeconomic status, age, sex, hepatitis viruses manifest the disease symptoms, viruses commonly observed in reported HAAA cases. The review also contains various detection methods for the causative agent of HAAA, clinical features relating to it and various options for its therapy In addition to the above listed projects, Dr. Idrees is also senior author on several manuscripts that submitted to high impact factor journals such Journal of Hepatology (IF 10.401), PlosOne (IF 3.534) etc and are currently under review. These research activities include differential expression profiles of hepatic microRNAs in chronic hepatitis C patients, identification of novel mutations an the entire genome of dengue virus, Physico-chemical Conservancy Analyzer: A Novel tool to Pin-Point Functional Domains in Multiple sequence alignments and studies on various circulating miRNAs as biomarkers for HCV disease and treatment outcome.

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OTHER IMPORTANT CONTRIBUTIONS: APPLIED RESEARCH

(A) Teaching Experience at National Centre of Excellence in Molecular Biology, University of the Punjab:

1. Molecular Virology (4 credit hours) to PhD/M. Phil Research Scholars (2003 – 2015 & 2019-2020).

2. Advance Course on Real-time PCR (4 credit hours) to PhD Research Scholars (2006 – 2015 & 2019-2020).

Demonstrator:

Viral Nucleic acid (DNA/RNA) extraction, cDNA synthesis, PCR, RT-PCR, Real-time PCR, qPCR, SSCP, RFLP, Cloning, sequencing etc to PhD Research Scholars (2001 – 2014).

(B) External Examiner (PhD/M. Phil level): 1. Baqai Medical University Karachi 2. University of Animal & Veterinary Sciences (UVAS) Lahore 3. Liaqat University of Medical & Health Sciences (LUMHS) Jamshoro Sindh. 4. Hazara University Mansehra KPK 5. Atta Ur Rahman School of Applied Biosciences (ASAB) National University of Science &

Technology (NUST) Islamabad. 6. Lahore University Lahore, Punjab Pakistan. 7. Government College for women University Lahore 8. University of the Gujrat

(C) Other Expertise, Clinical Trials My laboratory is also engaged in three clinical trials on HCV and HBV patients with three pharmaceutical sponsors such as MACTER International, GETZ Pharma and Biocare Pharma. (D) Future Research I am particularly interested in basic collaborative research involving virus-host interactions in host pathogenesis, viral transmission, viral persistence, various immune responses and antiviral resistance associated with hepatitis C virus and hepatitis B virus infections with national and international research institutes/hospitals. (E) Industrial Relevance Our research is highly relevant to industries interested in detection of infectious diseases such as HCV, HBV, HGV etc., production of recombinant HBV vaccines, understanding HCV replication, the various functions of HCV proteins and mechanisms of interferon resistance, insulin resistance and type 2 diabetes. With this knowledge, specific detection, prevention and improved therapies may be established for hepatitis viruses.

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e. EXPERIENCE TO SUPERVISE PHD STUDENTS LEADING TO SUCCESSFUL GRANT OF PH. D DEGREES TO STUDENTS.

Dr. Idrees has more than 18 years supervisory experience of PhD students leading to successful grant of Ph. D degrees. Till date he has supervised 22 PhD & 53 M. Phil students and is currently supervising 11 PhD and 2 M. Phil research scholars. i. List of PhD Students Supervised by Dr. Muhammad Idrees and awarded PhD

degrees:

S.NO. NAME OF STUDENT

TITLE OF THESIS NAME OF UNIVERSITY

REGISTRATION

DATE

1 Muhammad Zubair

Development of DNA Vaccine vectors against hepatitis C virus genotype 3a: The most prevalent genotype circulating in Pakistan


2004-2009

2 Shazia Rafique

Generation of virus like particles as potentional therapeutic vaccine for hepatitis C virus using enevelop glycoproteins of Pakistani strains


2004-2009

3 Sobia Manzoor A study of Molecular mechanisms of hepatitis C induced liver fibrosis


2004-2009

4 Shahida AR Shah

Association of Aplastic Anemia with Positive Viral Hepatitis Serology, and its outcome after Immunosuppressive Therapy

SZMC/University of the Punjab

2007-2012

5 Samia Afzal Study of inhibition of interferon inducible genes by dephosphorylation of E2 Envelope Gene of HCV Genotype 1a

University of the Punjab 2007-2013

6 Irshad ur Rehman

Molecular Cloning and Sequencing of Entire HCV Genotype 3a cDNA of Pakistani Isolates and their Quasispecies Analysis


7 Sadia Butt Establishment of stable cell lines expressing proteins of HCV Genotype 3a Pakistani isolate and their Molecular Characterization


8 Abrar Hussain Cloning and Sequencing of full length Pakistani HCV-1a & its Quasi Species Analysis


9 Sabeen Sabri Studies on the role of NS3 and NS5a Non-structural genes of Hepatitis C Virus genotype 3a local isolates in apoptosis


10 Bushra Khubaib

Identification of IL28-B genetic variations associated with virological response of interferon therapy in chronic HCV infected patients


2011-2015

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11 Sana Saleem Interferon Inducible Genes inhibition by Dephosphorylation of E2 Envelope Gene of HCV-1a genotype


12 Madiha Akram

Full length genome sequence analysis of dengue virus serotype-2 isolated in Pakistan and its genome variability studies


13 Zareen Fatima Correlation of sequence heterogeneity of Dengue Virus with disease severity


2011-2016

14 Azeem Mehmood Butt

Identification and expression profiling of microRNAs in hepatitis C patients using next generation sequencing


15 Muhammad Shahid

Identification and correlation analysis of host genes expression in response to Hepatitis C Virus infection and therapy


16 Dr. M. Akram Bajwa

Correlation of dup A, ice A and hom B genes of Helicobacter pylori in Pakistani Helicobacter pylori isolates with gastroduodenal diseases


2014-2019

17 Dr. Nafisa Fatima

Interleukins 1, 2, 4 and 13 as Biomarkers in Beta-thalassemic Major Patients with and without type 1 Diabetes Mellitus.


2014-2019

18 Maria Maryam

Identification of DNA methylation profiles of promoter of Ras genes (H-ras, K-ras, N-ras) during HCV 3a genotype Infection in Pakistani population


2015-2020

ii. PhD Produced as Co-Supervisor:


TITLE OF THESIS NAME OF UNIVERSITY

REGISTR

ATION DATE

19 Dr. Saima Chaudhary

Correlation of Helicobacter Pylori in Dental Plaque and Gastric Mucosa of Dyspeptic Patients


20 Farkhanda Yasmin

Development of stable cell lines of Dengue NS3 gene and anti-viral compound analysis

University of Veterinary and Animal Sciences Lahore

2013-2018

21 Ghulam Zahra Jahangir

Cloning and expression studies on abiotic stress tolerance genes in Solanum Tuberosum.

Lahore College for Women University, Lahore

2014-2018

22 Mahwish Aftab

Molecular Characterization and Genome-wide Variability Studies of Full-length Genome of Hepatitis Delta Virus Circulating in Pakistan.

Lahore College for Women University, Lahore

2014-2018

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iii. List of PhD Students currently being supervised by Dr. Muhammad Idrees


TITLE OF THE SYNOPSIS STATUS SESSION

24 Ayma Aftab Identification of DNA Methylation profiles of promoter & regulatory genes during HCV 3a genotype infection

Thesis sent for external evaluation

2013-2018

25 Sadia Zahid

Study of methylation profile of promoters of IRS1 and IRS2 genes in Hepatitis C Virus 3a genotype induced diabetes patients from Punjab

Thesis sent for external evaluation

2014-2018

26 Rabia Nawaz Development of DNA-based vaccine Vectors against Hepatitis C Virus

Thesis submitted to University for further processing

2013-2018

27 Iram Amin Expression and Molecular Characterization of Antigenic Recombinant Protein encoded by Hepatitis Delta Virus Antigen of Local Isolate

Synopsis approved by ASRB. 2015-2020

28 Rakhtasha Munir

Production and Molecular Characterization of Recombinant Proteins Encoded by Dengue Virus Structural Genes of Local Serotype-2


29 Sameen Ahmed

Expression of Recombinant Conserved Antigenic Region of High Risk Human Papillomavirus E7 Oncogene in 293 Cell Lines.

Synopsis approved by ASRB.

2015-2020

30 Aysha Wajiha

Production of Conserved Antigen Recombinant E6 Oncoprotein from High Risk Human Papillomavirus in 293 Cell Lines


31 Javeria Rafique Rao

Association of HCV Genome Regions Variants with the outcomes of HCV Treatment

Thesis writing stage

2015-2020

32 Muhammad Usman Ashraf

CRISPR/Cas 13-Medicated Targeting of Hepatitis C Virus RNA in Eukarytic Cell

Thesis writing stage 2018-2020

33 Iqra Almas Dynamic of Genetic Variations and Molecular Epidemiology of Hepatitis B Virus infection

Synopsis approved by ASRB

2018-2020

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iv. List of M. Phil Students Supervised by Dr. Muhammad Idrees and awarded M. Phil degrees:

S.No NAME OF STUDENT

TITLE OF THESIS

NAME OF UNIVERISTY

SESSION

1 Attia Muqaddas

Cloning, sequencing and phylogenetic analysis of S-gene of hepatitis B virus


1999-2001

2 Maqsood Anwar

Occult hepatitis B virus infection in hepatitis C positive patients


1999-2001

3 Sajjad Iqbal Genetic basis of Isoniazid and Pyrazinamide drugs resistance in Mycobacterium tuberculosis


2000-2002

4 Sana Ullah Khan

Common genotypes of hepatitis C virus circulating in Pakistan


2001-2003

5 Muhammad Hashim Raza

Detection of hepatitis G virus RNA in patients with hepatitis B virus, hepatitis C virus, Non-A-E and in general population using polymerase chain reaction


2001-2003

6 Dr. Ahmad Bilal Waqar

Detection of GB virus C/HGV replication sites in different human tissues using molecular techniques


2001-2003

7 Dr. Ejaz Khan

Role of mutations in NS5A genes of hepatitis C virus genotype 3a isolates and its correlation with treatment response


2002-2004

8 Abdul Ghafoor Khan

Mutational studies within E2 gene of hepatitis C virus genotype 3a isolates and its correlation with treatment response


2002-2004

9 Samia Afzal Molecular identification, cloning and sequence studies of NS5A gene of hepatitis C virus genotype 3a


2002-2004

10 Fouzia Irshad

Molecular identification, cloning and sequence studies of E2 gene of hepatitis C virus genotype 3a


2004-2006

11 Nighat Yasmin

Molecular identification, cloning and sequence studies of NS3 gene of hepatitis C virus genotype 3a


2004-2006

12 Muhammad Jamil

Detection of Helicobacter pylori in liver tissues of hepatitis C virus infected patients and its possible role in the development of cirrhosis and hepatocellular carcinoma


2004-2006

13 Saifur Rehman

Common genotypes of hepatitis C and hepatitis B virus circulating in Afghanistan and its comparision with that of Pakistan.


2004-2006

14 Aaliya Tariq Development of software based on various HCV genes, chemokines/cytokines in relation to antiviral treatment out come


2005-2007

15 Ashiq Hussain

Insilico study of pathogenecity and human gastric colonization of


2005-2007

16 Muhammad Tahir Iqbal

Cloning, sequencing and phylogenetic analysis of hepatitis E virus Pakistani isolates from two mini outbreaks


17 Sajjad Ullah Association of HCV NS5a mutations with Interferon plus ribavirin standard therapy outcome


2007-2009

18 Zunaira Awan

Detection of ymmd mutations in hepatitis B virus infected patients and its role in lamuvidene


2007-2009

54


resistance

19 Sadia Butt Changing pattern in HCV genotypes circulating in Pakistan in last 10 years


2007-2009

20 Mehwish Aftab

Establishment of cell-lines expressing E2 gene of HCV genotype 3a Pakistani isolate


2008-2010

21 Madiha Akram

Mutational analysis studies of NS5A gene of HCV-3a and their impacts on treatment responses


2008-2010

22 Bushra Khubaib

Development of cell-lines expressing NS5A gene of HCV genotype 3a Pakistani isolate


2008-2010

23 Muhammad Ali

NS5B genome based HCV genotyping and evolutionary analysis using modern Bio-informatics tools


2008-2010

24 Liaqat Ali Toxicological analysis and anti-viral effect of Kaolin derivatives in different cell-lines against HCV


2008-2010

25 Bisma Rauff Cloning and expression of E2 gene of HCV-1a in a mammalian expression vector pCDNA3.1 and its transient expression


2008-2010

26 Zareen Fatima

Molecular characterization of dengue virus Serotypes/genotypes circulating in Dengue outbreak-2011-Punjab Pakistan


2008-2010

27 Mahrukh Naudhani

Cloning and expression of NS5A gene of HCV genotype 3a in pCDNA3.1 expression vector and its transient expression


2008-2010

28 Sadaf Badar Mutational studies within E2 gene of hepatitis C virus genotype 3a isolates and its correlation with treatment response


2008-2010

29 Azeem Butt

Avian Influenza A (H9N2): Computational Molecular Analysis and Phylogenetic Characterization of Viral Surface Genes and Proteins Isolated between 1997 and 2009 from the Human Population


2009-2010

30 Sana Saleem Cloning & Expression of HCV-E2 gene of Genotype 3b local isolate & its comparison with other genotypes


31 Madeha Fayyaz

Expression of HCV-3a envelope protein in mammalian expression vector


2010-2011

32 Ayma Aftab Detection and Bio-informatics studies of mutations in Rifampicin and Isoniazid Resistant Strains of Mycobacterium Tuberculosis in Pakistan


33 Fatima Arshad Butt

Sequence-based genotyping of HDV and its correlation with HBV genotypes circulating in Pakistan


34 Naveed Ahmad

Transient Expression of NS3 protein of HCV-3a in mammalian expression vector


2010-2012

35 Saima Younas

Detection of Salmonella typhi in Dengue Virus Sero-positive samples


2011-2012

36 Muhammad Shahid

Pathway focused intrahepatic gene expression study of human Oxidative Stress and Anti-oxidant molecules in patients with Hepatitis C Virus induced Liver Fibrosis


2011-2012

55


37 Sayed Mohsin Raza

Pathway focused intrahepatic gene expression study of Inflammatory Cytokines & Receptors and Extracellular Matrix and Adhesion Molecules in patients with Hepatitis C Virus induced Liver Fibrosis


2011-2012

38 Rabia Nawaz Toxicological analysis and anti-viral effect of different natural compounds in different cell-lines against HCV


39 Noman Sharif

Expression of envelope genes of HCV-1a in a mammalian expression vector pCDNA3.1


2011-2013

40 Sara Basharat

Studies on gene expression Inflammatory Cytokines in patients with Hepatitis C Virus 1a induced Liver Fibrosis using pathway focused Mass Array


41 Tanzeel Yousaf

Establishment of Recombinant Huh-7 Mammalian Cell Line Expression Vector of NS5A Gene of HCV Genotype3a (Pakistani Isolate)


42 Wajeeha Naz Construction of a Mammalian Expression Vector Expressing NS5B Gene of HCV-3a Pakistani Isolate


43 Anam Saghir Studies on the role of genetic variation in SNP rs28416813 & SNP rs 4803219 in interferon resistance in chronic HCV infected patients


44 Hamna Imtiaz

Identification of blood miRNAs 141, 21, 126, 124 & 320 as a key to hepatitis C disease research


2013-2015

45 Iqra Almas Studies on circulating miRNAs-122, 134,198, 1, Let 7a, 7b & 7e as biomarkers for HCV disease and treatment outcome


46 Faiza Shams Genetic variation in SNP rs28416813 & SNP rs 4803219 in anti-viral resistance in chronic HCV infected patients


47 Iqra Ghaffar Role of different miRNAs on hepatitis C disease outcome


2014-2016

48 Hafiza Idrees Testing of different anti-viral drugs in cell culture University of the Punjab

2014-2016

49 Sana Usman Expression of SOCS1 gene in naïve and direct acting anti-viral drugs treated hepatitis C virus of district Lahore

CAMB, University of the Punjab

2015-2017

50 Beenish Saddique

Assessment of the cytotxic effect of sulfonamide compound on HEPG-2 cell lines


2015-2017

51 Braira Wahid

SOCS-1 mRNA in Study of the expression of interferon resistant, interferon treated and interferon ineligible Sofosbuvir treated HCV patients of District Lahore


2015-2017

52 Maham Saeed

Downregulation of Apoptotic Gene P53 Due to oncogenic E6 protein of High Risk Human Papillomavirus Type 16

CEMB, University of the Punjab

2018-2020

53 Ali Hassan Prediction of B cells and T cells epitopes for designing of tetravalent Dengue Vaccine

CEMB, University of the Punjab

2018-2020

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f. EXECUTED MAJOR RESEARCH PROJECTS:

Dr. Idrees has capabilities for mobilization of financial resources from National and International funding agencies. He has executed major research projects and have been the principal/co-principal investigator of several research projects funded by national and international funding agencies, namely Ministry of Science & Technology, Higher Education Commission, EMRO-WHO, State Department (Pak-USA) and Planning Commission of Pakistan. Further, under his direct supervision at Hazara University, his team prepared and submitted a PC1 for the “Uplifting of Academic and Infrastructure facilities of Hazara University” that was approved at a cost of Rs. 1702 Million by Planning Commission of Pakistan in his second year of 2018.

Dr. Idrees was Principal Investigator/Principal Researcher of the following Research Projects: COMPLETED PROJECTS

1. Establishment of Molecular (PCR-based) Diagnosis of infectious & genetic diseases Laboratory at Centre for Applied Molecular Biology (2001-2004, Ministry of Science & Technology PC1 PKR 39.46 Million). PI

2. Identification and transcriptome profiling of novel microRNAs in hepatitis C virus induced liver diseases by next generation sequencing (NGS) of small RNA cDNA libraries. (2013-2016; NRGPU PKR 3.37 Million). Project No. 20-2098. PI

3. Studies on Streamlined Molecular Approaches to Hepatitis C: Implications for Patient Management and Public Health. -(8/9/2015-2018; NRGPU PKR 4’520’288). PI

4. Development of Dengue Assays based on ELISA and PCR Diagnosis Systems for reproduceable and early detection of Dengue Virus. Lumpus Provision for Research, University of the Punjab, Lahore. 2016-17; PKR 10’00’000. PI

5. Streamlined Analysis of Dengue Virus Genome, Serotypes, Genotypes & Heterogeneity: Application to Molecular Epidemiology and Clinical Studies. University of the Punjab, Lahore. 2015; PKR 2’00’000. PI

6. Studies on the levels of Serum IL-8 and mutational analysis in the E2-PePD and NS5a-PKR-BD in Interferon-α responsive and non-responsive HCV patients (29/9/2003-30/9/2005, Pak-US co-operation on Science & Technology USD 11.72 Million PKR 11’720’000 ). Co-PI & Principal Researcher

7. Role of IL-8 and mutations in E2 & NS5A genes of Hepatitis C Virus in resistance to anti-viral therapy (04/03/2004-11/12/2005, EMRO-COMSTECH, WHO.USD 15,000; PKR ). Co-PI & Principal Researcher.

8. Prevalence of bovine tuberculosis in human, animal population and animal food products (HEC, 2004-2006; PKR 2.573 Million) Co-PI

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9. Modifier Chemical Cocktail’ and Its Role in Skin Tissue Regeneration in Mammals (2010; Higher Education Commission PKR 0.50 Million). Dr. Sherkhli (PI); Dr. Muhammad Idrees- (Co-PI)

10. Isolation, amplification and characterization of the complete Envelope gene sequences of all the four serotypes of Dengue virus, prevalent in Pakistan; a first step towards tetravalent vaccine development against dengue. Higher Education Commission. PKR 0.50 Million; Project Start Date: 04-01-2014. Dr. Amjad Ali (PI); Dr. Muhammad Idrees (Co-PI).

11. Expression and mutational analysis of p53 gene in breat cancer patients of Pakistan. Higher Education Commission. PKR 0.50 Million; Project Start Date: 25-06-2016 Dr. Haleema Sadia (PI); Dr. Muhammad Idrees (Co-PI).

12. Studies on the efficiency of newly developed direct acting antiviral drugs (DAAs)in chronic HCV patients. Higher Education Commission. PKR 0.50 Million; Project Start Date: 30-03-2016 Dr. Sajjad Ullah (PI); Dr. Muhammad Idrees (Co-PI).

13. Identification and characterization of novel fusion transcripts in neuroblastoma cell lines using transcriptome sequencing data. Higher Education Commission. PKR 0.50 Million; Project Start Date: 04-03-2016. Dr. Hafiza Fakhera Ikram (PI); Dr. Muhammad Idrees (Co-PI).

14. Expression of different Immunoreactive forms of the hepatitis C Virus NS5A recombinant protein and their use for development of ELISA assay. Higher Education Commission. PKR 0.50 Million; Project Start Date: 02-02-2016. Dr. Amjad Ali (PI); Dr. Muhammad Idrees (Co-PI).

PROJECTS IN PROGRESS/PROCESS:

15. Studies on Efficient Molecular Approaches to Hepatitis C & their Implications for Patient Management-(2015-2018; NRGPU PKR Million). Project No. 20-3708. PI

16. Development of a single chimeric Dengue Virus DNA Vaccine and study of its immunogenicity and response against local Dengue Serotypes." at total cost of Rs.7561256/- Date: 02-06-2018; Project No. 8458. Co-PI

17. Robust production of recombinant collagen and elastin proteins in serum-free adapted 293T cell line using Ubiquitionous Chromatin Opening Elements containing lintiviral Vectors-(2017-2020; NRGPU, PKR 4195324) Project No. 6741. Co-PI.

18. Application of Streamlined Molecular Approaches to Hepatitis C: Implications for Public Health and Patient Management. (PAK US JOINT COMMITTEE ON SCIENCE &TECHNOLOGY COOPERATION). PKR 53 Million. PI. Awarded

19. Development of HCV Vaccines and Experimental Animal Model for Chronic HCV patients. (2008-2013; PC1; PKR 37.49 Million). PI. Accepted for Award

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DEVELOPMENTAL PROJECTS (Completed/On-going): 20. Uplifting of Academic and Infrastructure facilities of Hazara University that was

approved at a cost of Rs. 1702 Million by Planning Commission of Pakistan. 19-03-2018 to18-03-2021

21. Construction of two Academic Blocks at Hazara University by Provincial Government at a cost of 65.0 Million

22. Completion of Left-over work of eight academic blocks (each one with a covered area of about 49,000 square feet) below the approved cost of 662.45 Million (HEC Grant) within a record time of one and a half year

INTERNAL PROJECTS COMPLETED: 23. The Dilemma of Zoo prophylaxis: Cattle ownership can increase rather than reduce

prevalence of malaria in Pakistan (1994-96, University of Peshawar). PI-Internal 24. Molecular studies of Multiple Drug Resistant Genes: Testing of Mycobacterium

tuberculosis to commonly used anti-TB drugs and Detection of Rifampicin Resistant Mutants by PCR-SSCP (1996-1999, CEMB). PI-Internal

25. Establishment of Molecular Diagnostic Laboratory in Private Sector (1999-2000, The Medical Laboratories Lahore). PI-Internal

26. To find out the common genotypes of HCV present in Pakistan (1999-2000, The Medical Laboratories Lahore). PI-Internal

27. Comparison of various Genotyping & Serotyping assays for the accurate detection of HCV Genotypes (1999-2000, The Medical Laboratories Lahore). PI-Internal

28. Evaluation of various Commercial procedures/kits/systems for the quantification of HCV Virus. (1999-2000, The Medical Laboratories Lahore). PI-Internal

29. Influence of HCV Genotypes on Viral load and Disease outcome. (1999-2000, The Medical Laboratories Lahore). PI-Internal

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g. EXPERIENCE OF WORKING WITH INTERNATIONAL BODIES/ INTERNATIONAL EXPOSURE THROUGH PARTICIPATION IN WORKSHOPS, SEMINARS, CONFERENCES HELD OUTSIDE OF THE COUNTRY:

Dr. Idrees has experience of working with International Bodies and have International exposure through participation in workshops, seminars, and conferences held at home and outside of the country. Participation of Dr. Idrees in Conferences, Seminars, Workshops and Trainings International (abroad) are given below:

1. Participated in 25th ECCMID European Congress of Clinical Microbiology & Infectious Diseases organized by European Society of Clinical Microbiology & Infectious Diseases at Bella Center Copenhagen, Denmark from 25-28 April 2015.

2. European association for the study of Liver Diseases-EASLs-50th International liver Congress. Vienna (Austria), 22-26 April 2015.

3. ARAB HEALTH, 27 – 30 January 2014; Dubai International Convention & Exhibition Centre, United Arab Emirates. My Unique Reference Number was (URN): 196527.

4. Software & hardware training on SaCycler newly developed State-of-the-art Real-Time PCR-April 11-15, 2013-Como-Italy

5. Technical training on Automated Nucleic Acid Extraction System-Magnesia® Real-time PCR System-April 09-10, 2013 Anatolia Geneworks Istanbul-Turkey.

6. September 15, 2006; Certificate of Course Mastery recognized by the OFFICE OF HEALTH & SAFETY, CDC, USA for demonstrating a thorough understanding and ability to apply the principles of “SAFETY SURVIVAL SKILLS PT-1: GENERAL RESPONSIBILITIES”. Atlanta-USA

7. September 18, 2006; Certificate of Course Mastery recognized by the OFFICE OF HEALTH & SAFETY, CDC, USA for demonstrating a thorough understanding and ability to apply the principles of “SAFETY SURVIVAL SKILLS PT-2: LABORATORY SAFETY”. Atlanta-USA

8. November 04, 2006; Certificate of Course Mastery recognized by the Office of Health & Safety, CDC, USA for demonstrating a thorough understanding and ability to apply the principles of “SUPERVISORY SKILLS”. Atlanta-USA

9. November 08, 2006; Certificate of Course Mastery recognized by the Office of Health & Safety, CDC, USA for demonstrating a thorough understanding and ability to apply the principles of “ACCIDENT INVESTIGATION”. Atlanta-USA

10. November 08, 2006; Certificate of Course Mastery recognized by the Office of Health & Safety, CDC, USA for demonstrating a thorough understanding and ability to apply the principles of “OCCUPATIONAL EMERGENCY PROGRAM”. Atlanta-US

11. Training on Biosafety level 1 (BSL1), BSL2, BSL3 and BSL 4, CDC USA, 2006. 12. Training on Real-time PCR Kit/procedure principle-ITALY in the year 2013.

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h. EXPERIENCE OF ORGANIZING EVENTS SUCH AS WORKSHOPS, SEMINARS, CONFERENCES AT INTERNATIONAL LEVEL WITHIN THE COUNTRY IN THE FIELD OF HIGHER EDUCATION

1. Organized a Seminar and hands-on training on Cepheid (USA) next generation PCR “Real-time PCR (GenXpert solution) results for Real-time Patient Managements” Wednesday 28th October at 9:30 am to 1:00 PM at CAMB, University of the Punjab in collaboration to Cepheid Middle East & GMS.

2. Organize/Conduct “Evaluation & Progress in Research of Molecular Virology Lab” –From 2001-continue each year.

3. Participated in “DNA Strategy Symposium” organized by British High Commission in collaboration to Punjab Forensic Science Agency held from 18th – 19th January 2016 in PC-Lahore-Pakistan.

4. Participated in “5th Invention to Innovation Summit-2016” March 02-04, 2016 organized by Office of Research Innovation & Commercialization (ORIC), University of the Punjab Lahore.

5. Workshop on Dengue Management sponsored by the Higher Education Commission & organized by Institute of Agricultural Sciences University of the Punjab Lahore December 21, 2015.

6. Dengue Seminar Dengue Research Group, University of the Punjab Wednesday, 4th November 2015.

7. Dengue Seminar organized by Dengue Research Group, University of the Punjab Wednesday, March 2015.

8. Training workshop on “New Trends in Biomedical Research-Grant writing” at Centre of Excellence in Molecular Biology, University of the Punjab. December 22, 2014.

9. Lahore Biotech Cluster Meeting: August 18, 2014. Department of Biological Sciences Forman Christian College University Lahore.

10. Participated in 37th Annual Conference of Pakistan Association of Pathologist/2nd Joint Conference of Societies of Pathology held at Pearl Continental Hotel, Lahore from 20th-22nd December, 2013.

11. The 37th annual conference of the Pakistan Association of Pathologists/2nd Joint Conference of the Societies of Pathology is being held in Lahore from the 20th to the 22nd of December 2013.

12. 11th Biennial Conference Molecular Biosciences Challenges and Opportunities November 25–28, 2013. University of the Punjab Lahore-Pakistan.

13. International Conference on Dengue & its Control [Post DF/DHF Otbreak] Lahore-Pakistan. 27-29 February 2012 Organized by World Health Organization (WHO) & Government of the Punjab.

14. Participated in 36th Annual Conference of Pakistan Association of Pathologist/1st Joint Conference of Societies of Pathology held at Islamabad from 21st-23rd December, 2012.

15. Participated in OIC Thematic Workshops on “DNA/Emerging Vaccine & Production Challenges” from Sep 03-09, 2007, Islamabad Pakistan Organized by US EMBASSAY & US DEPARTMENT OF HSS.

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16. Participated in “Training workshop on Bio-security and Biosafty”, Islamabad, June 18-19, 2007 Organized by US Department of HHS and US Embassy in collaboration to NARC.

17. Got Special training on Biosafty and Bio-security, CDC, USA 2007. 18. 2nd National Conference on Infectious Diseases from March 25-27, 2005, Lahore. 19. Participated in 7th International Symposium Workshop on the application of Molecular

Biology Research in Agriculture and Health, March 27-31, 2004, held at CEMB, Lahore, Pakistan and presented paper on “Genome variability studies in HCV”.

20. 7th Biennial Conference on “Trends in Biochemistry & Molecular Biology, April 2-5, 2003 Institute of Biochemistry & Biotechnology, University of the Punjab.

21. Nineteen Annual Congress of Pakistan Society of Gastroenterology and GI endoscopies. February 28 to March 02, 2003. Lahore, Pakistan.

22. Fifth International Symposium on Typhoid Fever & Other Salmonellae’s February 6-7 (2002). Department of Pediatrics, The Agha Khan University Hospital Karachi, Pakistan.

23. Workshop on Molecular Methods in the Epidemiology & Diagnosis of Typhoid, February 4-5, 2002, Department of Pediatrics, The Agha Khan University Hospital Karachi, Pakistan.

24. 2nd International Science Conference, 26-28 October 2000, Institute of Chemical Engineering & Technology University of the Punjab, Lahore.

25. 20th Pakistan Congress of Zoology, March 29-31, 2000 University of Sindth, Jamshoro, Pakistan and presented a paper on comparison of serotyping genotyping systems.

26. Third International Biennial Conference of Pakistan Society for Microbiology, March 28-30, 2000, Lahore, Pakistan, and presented a paper on HCV genotyping.

27. 19th Pakistan Congress of Zoology, April 19-21, 1999, held at the premises of NARC Islamabad and presented a paper on “PCR-SSCP:

28. National Conference on Tuberculosis Update November 8, 1998, organized by Pakistan Chest Society Faisalabad Chapter and delivered a lecture on “Detection and Identification of M. tuberculosis by DNA amplification and conventional culture methods in local samples”.

29. 18th Pakistan Congress of Zoology April 20-22, 1998, held at Dept. of Zoology, University of the Punjab and presented a paper on “Testing of M. tuberculosis susceptibility to first-line anti-TB drugs in Lahore, Pakistan.

30. International Symposium Workshop on Genomics and Computational Analysis, October 16-18, 1997 at CAMB, University of the Punjab.

31. 16th International Conference on Tuberculosis and Chest Diseases, March 8-11, 1998, organized by Pakistan anti-TB Association and Institute of Chest Medicine King Edward Medical College, Lahore, Pakistan and delivered a lecture on “A comparative study to understand the usefulness of conventional culture method and PCR based diagnosis of tuberculosis in local samples”.

32. Participated in 5th International Symposium Workshop on the application of Molecular Biology Research in Agriculture, Health and Environment, October 14-15, 1997, held at CEMB, Lahore, Pakistan and presented paper on “The Dilemma of Zoo prophylaxis:”

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OTHER NATIONAL/INTERNATIONAL CONFERENCES (Participated)

1. Imtiaz H, Idrees M. Circulating microRNA expression profiling in HCV infected patients: A novel insight into diagnostic and therapeutic research. In: International Symposium on Advances in Molecular Biology of Plants & Health Sciences. December 29-31, 2015, organized by CEMB, University of the Punjab Lahore-Pakistan. Page-70.

2. Shahid M, Idrees M. Correlation of clinical markers, viral load with liver fibrosis stages and grades in chronic HCV patients with genotype 3a. In: International Symposium on Advances in Molecular Biology of Plants & Health Sciences. December 29-31, 2015, organized by CEMB, University of the Punjab Lahore-Pakistan. Page-73.

3. Amin I, Idrees M. Vertical transmission of dengue by Ades sygepti. In: International Symposium on Advances in Molecular Biology of Plants & Health Sciences. December 29-31, 2015, organized by CEMB, University of the Punjab Lahore-Pakistan. Page-75

4. Afzal S, Idrees M. Global consensus sequence development of dengue virus for peptide-based vaccine design against local dengue virus. In: International Symposium on Advances in Molecular Biology of Plants & Health Sciences. December 29-31, 2015, organized by CEMB, University of the Punjab Lahore-Pakistan.

5. Samia Afzal, Muhammad Idrees, Iqra Almas, Shazia Rafique, Iram Amin, Muhammad Shahid: Study of inhibition of interferon inducible genes by dephosphorylation of E2 envelope gene of HCV genotype 1a. 2017 14th International Bhurban Conference on Applied Sciences and Technology (IBCAST); 01/2017, DOI:10.1109/IBCAST.2017.7868053

6. Iqra Almas, Samia Afzal, Muhammad Usman Ashraf, Khadija Zahid, Muhammad Asif, Muhammad Idrees. Studies on circulating microRNAs: Members of Let-7 family and their correlation with Hepatitis C virus disease pathogenesis and treatment concerns. 2017, 14th International Bhurban Conference on Applied Sciences and Technology (IBCAST); 01/2017, DOI:10.1109/IBCAST.2017.7868051.

7. Khadija Zahid, Zahid Iqbal, Samia Afzal, Iram Amin, Muhammad Shahid, Muhammad Idrees. Development of Global Consensus Sequence & Identification Of Conserved Domains In Hcv Ns5b Protein. CEMB, 2017, 2nd International Symposium on Advances in Molecular Biology of Plants and Health Sciences.21-23 November, 2017, Lahore-Pakistan.

8. Samia Afzal, Muhammad Idrees, Iram Amin, Muhammad Shahid. Global consensus sequence development of dengue virus for peptide based vaccine design against local Dengue Virus. CEMB, 2015, 1st International Symposium on Advances in Molecular Biology of Plants and Health Sciences, 29-31 December, 2015, Lahore-Pakistan.

9. Hamna Imtiaz, Muhammad Idrees. Circulating micro-RNAs expression profiling in HCV infected patients: A novel insight into diagnostic and therapeutic research. CEMB, 2015, 1st International Symposium on Advances in Molecular Biology of Plants and Health Sciences, 29-31 December, 2015, Lahore-Pakistan.

10. Muhammad shahid, Muhammad Idrees, Iram Amin, samia Afzal. Correlation of clinical marker, viral load

11. with liver fibrosis stages and grades in chronic HCV patients with genotype 3a. CEMB, 2015, 1st International Symposium on Advances in Molecular Biology of Plants and Health Sciences, 29-31 December, 2015, Lahore-Pakistan.

12. Iram Amin, Muhammad Idrees, Muhammad shahid, Samia Afzal. Vertical Transmission Of Dengue By Aedes aygepti. CEMB, 2015, 1st International Symposium on Advances in Molecular Biology of Plants and Health Sciences, 29-31 December, 2015, Lahore-Pakistan.

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i. DEMONSTRATED EXPERIENCE IN LEADERSHIP: A distinguished management executive, higher academic leader, eminent researcher, higher education manager like Dr. Idrees with a demonstrated track record of leadership in higher education institutions/universities, and career profile with diverse training and experience. He has proven and well-established credential and experiences of about 20 years as Vice Chancellor, Director, Associate Professor, Incharge Commercial Diagnostic Lab (Public sector), Group Leader of a Research Group etc. As a Vice Chancellor of Hazara University for about three years, his experience of academic planning, academic program review & curriculum development; supervision of budget preparation for the academic and academic-related programs of Hazara University, allocating and reallocating funds and resources to and from these programs; provision of leadership in creating and maintaining academic standards and policies; chair the Syndicate, Academic Council of Hazara University, the General Board of the Faculties and the Finance Committee of the Council; Provide leadership, academic and administrative to the whole University; Represent the University externally, both within the country and overseas; Secure a financial base sufficient to allow the delivery of the University’s mission, aims and objectives. Further, having post doctorate from USA and FRCPath from United Kingdom can provide the required academic leadership and administrative experience to be Vice Chancellor of any university of Khyber Pakhtunkhwa. He is/was:

1. Vice Chancellor, Hazara University Mansehra, Khyber Pakhtunkhwa- From July 28, 2016- to date

2. Director, Center for Applied Molecular Biology, University of the Punjab Lahore-From July 1, 2015-July 27, 2016.

3. Incharge Disease Diagnostic Facility, CAMB, Ministry of Science & Technology Government of Pakistan- From 2001- 2009.

4. Group Leader, Infectious Diseases & Molecular Virology Group CEMB, University of the Punjab- From 2000- continue

5. Member Senate, Hazara University 6. Member senate, Women University Mardan 7. Member Senate, University of Baltistan, Gilgit Baldistan 8. Convener, Hazara University Syndicate 9. Member Syndicate, University of Malakand 10. Convener/Chairman, Hazara University Selection Board 11. Convener/Chairman, Academic Council Hazara University Mansehra-KP 12. Convener/Chairman, Board of Faculties (BOF) of (i) Health Sciences & (ii) Law &

Administrative Sciences, Hazara University Mansehra-KP 13. Member of a team of four Scientist designated by CDC, USA to visit Pakistan

(2006-07) to meet with several public health officials from the Ministry of Heath and the National Institute of Health to discuss various collaborative public health programs

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and activities that will support the Field Epidemiology & Laboratory Training Program (FELTP).






19. Member Biosafety & Bioresource Committee University of the Punjab to review the research proposals of the faculty members and Ph. D scholars before submission to Higher Education Commission (HEC) and Pakistan Science Foundation (PSF). From 22-9-2015-ongoing.

20. Chairman/Convener, Purchase Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015- continue

21. Chairman/Convener, Technical Evaluation Committee, Centre for Applied Molecular Biology, University of the Punjab-Lahore. From 2015- continue

22. Convener/Chairman, Board of Studies (BOS), CAMB, University of the Punjab Lahore. From 2015-ongoing.

23. Member, Board of Faculty (BOF), University of the Punjab Lahore. From 2015-ongoing.

24. Member Board of Studies (BOS) Department of Genetics, Hazara University Garden Campus Mansehra, KPK-From 2011-continue

25. Member, Academic Council University of the Punjab Lahore. From 22-8-2015-ongoing.



28. Memberships of International Societies:

Fellow Royal College of Pathologist (FRCPath) London United Kingdom (UK)-Member No. 20005720 American Society of Microbiology (ASM)-Member ID: 56817638

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European Society of Clinical Microbiology and Infectious Diseases (ESCMID)- Member ID: 122577

Hellenic Society of Virology International Society for Antiviral Research The Federation of European Microbiological Societies Zoological Society of Pakistan

29. Currently Editor of International Scientific journals such as

World Journal of Clinical Infectious Diseases Global Journal of Gastroentrology & Hepatology World Journal of Virology World Journal of Gastroenterology & Hepatology World Journal of Gastroenterology The Science Hepatoma Research Journal of Hepatitis Research

30. Reviewer of more than 20 International Scientific Journals (few are given below):

The Lancet Infectious Diseases Nature Emerging Infectious Diseases Virus Genes European J. Virological Methods, BMC Virology, BMC Infectious Diseases, Infection, Genetics, Evolution, Journal of Medical Virology

31. Reviewer of grants in the field of Health Biotechnology for Higher Education

Commission (HEC) of Pakistan.

32. Visiting Scientist of:

33. CDC, US Department of Health & Human Services, Atlanta, USA. 34. Ministry of Health Government of the Punjab. 35. Lahore General Hospital Lahore, Pakistan 36. Peshawar Medical Collage, Peshawar, NWFP, Pakistan 37. Dow University of Health Sciences, Karachi 38. Liaquat University of Medical & Health Sciences Jamshoro Sindh. 39. Hazara University Mansehra KPK

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j. NEW ORGANIZATIONS AND EXCELLENT TEACHING AND RESEARCH PROGRAMS DEVELOPED:

i. Established Batagram Campus of Hazara University under HEC Umbrella Project and started academic functioning from the Fall Semester 2018.

ii. Developed an excellent Teaching and Research program in Applied Molecular Biology & Forensic Sciences:

In 2015, Dr. Idrees along with his fellow prominent scientists have developed an excellent teaching and research program in Applied Molecular Biology & Forensic Sciences for which he is internationally recognized as an eminent molecular biologist. Details of his developed teaching & Research Programs are given below:

Sr. No. DEGREE DEGREE TITLE DEVELOPED FOR: 1 Graduate BS in Applied Molecular Biology and

Forensic Sciences University & HEC

2 M. Phil M. Phil in Applied Molecular Biology and Forensic Sciences

University & HEC

3 Ph. D Ph. D. in Applied Molecular Biology and Forensic Sciences

University & HEC

iii. New Programs Initiated at Hazara University: Ten modern and market oriented academic disciplines including Biotechnology, Forensic Sciences, Geology, Environmental Sciences, Pharmacy, Agriculture, Elementary Education, Medical Lab Technologies, were got approved from statutory bodies and launched in HU.

iv. Infrastructure Completion of leftover work of the Turkish NGO by constructing 8 Academic Blocks at HU

with the financial sponsorship of HEC, amounting to Rs. 662 Million, (covered area of 4’00’000 Sft), furnished with comfortable furniture and modern equipment for instruction and research.

Constructed two faculty hostels (one each for male & female faculty) with the financial sponsorship, amounting to Rs. 65.924 Million, of the HED/Provincial Government.

Completed Smart University Project (sponsored by HEC) just in 6 months record time and now Wi-Fi carpet coverage across the campus is provided with high speed bandwidth of 424 MB. Researchers are now able to access all digital resources at a very high speed anywhere in the campus. In next 1 year the facility will be extended to SAFE Campus and SMART Classroom.

A mega project for the uplifting of Academic and Infrastructure facilities of Hazara University has recently been approved at a cost of Rs. 1702 Million by Planning Commission of Pakistan.

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k. HONORS AND AWARDS

International

1. Young Scientist of the year award in Biology 2009 by Third World Academy of Science (TWAS) Italy.

National 2. PAS Gold Medal-2016 in Scientific Discipline “Emerging Technologies,

including Biotechnology, nanotechnology, Molecular Biology & Bioinformatics” by Pakistan Academy of Sciences.

3. Research Productivity Award (RPA) 2017 by Pakistan Council for Science & Technology (PCST)-Category C

4. Research Productivity Award (RPA) 2016 by Pakistan Council for Science & Technology (PCST)-Category B

5. Research Productivity Award (RPA) 2015 by Pakistan Council for Science & Technology (PCST)-Category C



8. Research Productivity Award (RPA) 2012 by Pakistan Council for Science & Technology (PCST)- Category C

9. Research Productivity Award (RPA) 2011 by Pakistan Council for Science & Technology (PCST)- Category F

OTHERS: 10. Performance Evaluation Award for the period of from 1-1-2015 to 31-12-2015 to the PhD

teacher by University of the Punjab Lahore-Obtained numbers 70 (100%) 11. Performance Evaluation Award for the period of from 1-1-2014 to 31-12-2014 to the PhD

teacher by University of the Punjab Lahore-Obtained numbers 68.5 (91.3%) 12. Performance Evaluation Award for the period of from 1-1-2013 to 31-12-2013 to the PhD

teacher by University of the Punjab Lahore-Obtained numbers 64 (85.33%) 13. Performance Evaluation Award for the period of from 1-1-2012 to 31-12-2012 to the PhD

teacher by University of the Punjab Lahore-Obtained numbers 68 (90.67%) 14. Gold Medal for Best Research Paper Presentation in 21st Annual congress of Pakistan

Society of Gastroenterology and GI endoscopes, March 25-27, 2005, Peshawar Pakistan.

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l. CONTRIBUTION TO PUBLIC SERVICE

1. Establishment of Molecular Diagnostic Facility for General Public: In May-2000 Ministry of Science & Technology (MoST) Government of Pakistan appointed me as Research Officer/Head of Molecular Diagnostics and given me a task to establish a Molecular Diagnostic Laboratory in public sector. Only in six months time I was able to establish Molecular (PCR-based) Diagnostic Laboratory for the detection of infectious & genetic diseases at Centre for Applied Molecular Biology (CAMB), MoST and provided general public with reliable, accurate, rapid and more economical diagnostic service for most prevalent infectious and genetic diseases. Currently my developed DNA based diagnostic laboratory at CAMB provides PCR-based Detection Methods for the diagnosis of HCV, HBV, TB, CMV, HSV (type 1&2), TB, typhoid, and HCV genotyping, HCV viral load, MDR-TB. On daily basis this Lab is receiving more than 250 samples from different tertiary care hospital of the country that is the largest number of samples in the country. From the last 16 years this laboratory is providing the Molecular Diagnostic Facilities for most of the infectious agents circulating in Pakistan at about 1/10th of the charges offered by other commercial diagnostic laboratories. This endeavor has also generated a source of income for the Centre and MoST. Present income of the Lab is about 5.0 million per month (60 million/year). In year 2013 an amount of 350 million was deposited to Government of Pakistan as generated revenue through MoST.

2. Developed Molecular Diagnostic Kits: As a part of these endeavors, to develop indigenous technologies, I have developed Molecular Diagnostic Kits for the qualitative detection of hepatitis B virus, hepatitis C virus, M. tuberculosis and HCV Genotyping at 1/10th the cost of the same kits manufactured by other companies in USA. This will help to overcome inherent constraints of practicing modern molecular tools for diseases diagnosis and encourage/contribute to the indigenous technologies in the area of Molecular diagnostics. All these kits are ready for marketing.

3. Patent: Idrees M. and Riazuddin S. PCR-based diagnostic kit for the detection of Hepatitis C virus. (2003) Pak#138719.

4. Helped in Prime Minister Program for Prevention & Control of Hepatitis Due to vital importance of viral testing, as gesture and the noble consent, the Centre offered and directed Dr. Idrees to provide Molecular Diagnostic Facilities (qualitative, quantitative and genotyping) to the patients booked for hepatitis B&C infection treatment in the 104 sentinel sites (teaching and district headquarter hospital) in the country under the PM’s Program for Prevention & Control of Hepatitis. From September 2006 to July 2008 he along with his group had performed more than 50,000 HCV quantitative/HCV genotyping and HBV quantitative tests free of charges to the poor patients. The market value of these tests is more than 113.00 million.

5. Services during Dengue Virus Outbreaks 2011-2016: Dr. Idrees was the sole Virologist/scientist of the country who was actively involved in the molecular identification and serotype detection of Dengue outbreak-2011. Till date his group is providing dengue detection & serotyping facility free of charges to all provinces referred by WHO. Dr. Idrees has been awarded Certificate of appreciation for excellent lab support in detection of dengue fever cases during last outbreak in the year 2011 and shield for his expert opinion for “Lab Services in Case Detection for Dengue Fever” on February 29, 2012 by WHO.

6. Services during Coronavirus SARS-CoV-2 Epidemic 2020: Dr. Idrees established a state-of-the-art laboratory for the diagnosis of SARS-CoV-2 causing COVID-19 in the start of the epidemic in Government sector and offered free of charge to general population. In addition he also developed Multiplex real-time PCR kits for the diagnosis of COVID-19.

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m. PROMOTION OF INDUSTRY-ACADEMIA LINKAGES

i. Collaborative Research: Dr. Idrees’s group is the first in the country to conduct studies on viral hepatitis and he is supervising a team of researchers to study the genome variations in hepatitis viruses and Dengue Virus. Dr. Idrees has made significant contributions to the field of Molecular Virology research. Specifically, Dr. Idrees’s virology work relates to eight main themes which consist of Establishment of assays/methods to study viruses, Studies on Virus Genome characterization, variability, Genotyping/ Serotyping, Studies on viral genes/proteins and their role in disease outcome using Bioinformatics tools, Virus Prevalence and Molecular Epidemiology, Studies on Anti-viral therapy: role of various viral & host factors in treatment response rates and Studies on Virus association with other abnormal conditions/Diseases. Dr. Idrees has participated in other studies which include diseases of respiratory system, metabolism, cancer, Stem cells & it’s Role in Skin Tissue Regeneration in Mammals, Zoo prophylaxis, etc. Our research is highly relevant to industries interested in detection of infectious diseases such as HCV, HBV, HGV etc., production of recombinant HBV vaccines, understanding HCV replication, the various functions of HCV proteins and mechanisms of interferon resistance, insulin resistance and type 2 diabetes. With this knowledge, specific detection, prevention and improved therapies may be established for hepatitis viruses.

ii. Clinical Trails

His laboratory had also been engaged in three clinical trials under his direct supervision on HCV and HBV patients with three pharmaceutical sponsors such as MACTER International, GETZ Pharma and Biocare Pharma.

iii. Developed Multiplex Real-time PCR tests kit for the specific diagnosis of COVID-19 Disease The test detects specific nucleic acid sequences from the genome of the nCoV-19/SARS-CoV-2 RNA dependent RNA polymerase RdRp gene and E-gene. We had already applied to Drug Regulatory Authority of Pakistan for Emergency Use Authorization (EUA) and hopefully will be granted in few weeks. Global Marketing (Pvt Ltd) Islamabad had already requested and collaborated for the marketing of these kits.

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n. PATENTS:

Idrees, M and Riazuddin, S. PCR-based diagnostic kit for the detection of Hepatitis C virus. (2003) Pak#138719.

o. BOOKS

1. HCV Therapy and Baseline IL-8 Levels: A Practical Approach-High levels of Baseline IL-8: A Risk Factor for Low Treatment Response in Chronic HCV Patients. By Haji Akbar, Muhammad Idrees, Khurram Shehzad

ISBN 10: 3639336186 ISBN 13: 9783639336184 Publisher: VDM Verlag Dr. Müller, 2011

2. Determination of HCV Genotypes in Khyber Pakhtankhawa: Molecular Epidemiology of Hepatitis C Virus genotypes in Province of Pakistan. By Amjad Ali, Muhammad Idrees, Habib Ahmad-LAMBERT Academic Publishing

ISBN 978-3-8465-8362-3 Publisher: LAMBERT Academic Publishing

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PART ‘B’ DESIRABLE EXPERIENCE

a. EXPERIENCE OF WORKING ON THE STATUTORY AUTHORITIES OF A UNIVERSITY SUCH AS BOARD OF STUDIES, ACADEMIC COUNCIL, SYNDICATE OR MANAGEMENT COUNCIL OR EXECUTIVE COUNCIL OF BOARD OF MANAGEMENT ETC.

Being a Vice Chancellor of Hazara University (the 2nd largest University of Khyber Pakhtunkhwa in term of number of students), he has experience of working on the Statutory Authorities of a university such as Board of Studies, Academic Council, Selection Boards, Syndicate, Senate, Executive Council of Board of Management etc. After assuming charge of permanent VC of Hazara University on July 28, 2016, he focused on the development, modernization and progress of the University. Six major achievements of this short era include framing and approval of Statutes-2016; the unprecedented construction of infrastructure (Eight academic blocks with a covered area of about 392,000 square feet), convening of promotion board for administrative officers & faculty; enhancement of transport facility; launching of ten modern and market oriented academic disciplines and beatification of the Campus. He was:

1. Member Senate, Hazara University Mansehra, Khyber Pakhtunkhwa

2. Member senate, Women University Mardan, Khyber Pakhtunkhwa

3. Member Senate, University of Baltistan, Gilgit Baltistan.

4. Convener Syndicate, Hazara University Mansehra, Khyber Pakhtunkhwa.

5. Member Syndicate, University of Malakand, Khyber Pakhtunkhwa.

6. Convener/Chairman, Finance & Planning Committee (F&PC), Hazara University Mansehra, Khyber Pakhtunkhwa.

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7. Convener/Chairman, Academic Council, Hazara University Mansehra, Khyber Pakhtunkhwa.

8. Convener/Chairman Selection Board, Hazara University Mansehra, Khyber Pakhtunkhwa.

9. Convener/Chairman, Board of Faculties (BOF), Hazara University Mansehra, Khyber Pakhtunkhwa

10. Convener/Chairman, Board of Studies (BOS), CAMB, University of the Punjab Lahore. From 2015-2016.

11. Member, Board of Faculty (BOF), University of the Punjab Lahore. From 2015-2016.

12. Member Board of Studies (BOS) Department of Genetics, Hazara University Garden Campus Mansehra, KPK-From 2011-2016.

13. Member, Academic Council University of the Punjab Lahore. From 22-8-2015-2016.



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b. DEMONSTRABLE EXPERIENCE OF HANDLING QUALITY ISSUES, ASSESSMENT AND ACCREDITATION PROCEDURES, etc.

Dr. Idrees has demonstrable experience of handling Quality issues, assessment and accreditation procedures etc. He was member of National Quality Assurance Committee (NQAC) of Higher Education Commission (HEC). The objectives of NQAC are to:

i. Facilitate HEC, Program & Institution Reviewers and University Administration in assessing

and adopting the Quality criteria ii. Track the effectiveness of the QA programs of HEC and proposing appropriate modifications iii. Update the Quality Assurance indicators, policies and guidelines of HEC in view of global

developments & local stakeholders’ feedback and proposing SoPs for their adoption iv. Identify future capacity building requirements for institutions to improve the services of

institutions towards a growing and more diversified tertiary education sector.

Furthermore, Dr. Idrees has obtained training on “Quality Management Consultants” containing Internal Auditing, Corrective & Preventive Action conducted by QMC Lahore in year 2009.

In addition, Dr. Idrees was Technical Manager Molecular Diagnostic Laboratory (MDL) of Centre for Applied Molecular Biology, Ministry of Science & Technology during its ISO certification & accreditation. I as a Technical Manager of the MDL-CAMB was responsible for but not limited to: 1. Direct the systematic actions necessary to demonstrate that a product or service meets the specified requirements for quality as set forth by ISO 9001-2008 & ISO 15189. 2. Establish, implement, and maintain a quality system appropriate to the scope of the laboratory’s activities. 3. Reviews, documentation, revision of policies, systems, programs, procedures and instructions to the extent necessary to assure the quality of the test and/or calibration results. 4. Establish a program for the maintenance and calibration of laboratory equipment. 5. Establish and maintain an internal/external proficiency testing program to monitor performance and identify areas for improvement. 6. Ensure that quality control measures are performed on critical reagents prior to use. 7. Maintain documentation pertinent to receipt, preparation, and testing of all reagents. 8. Planning and organizing audits as required by the schedule and requested by management. 9. Management of technical operation of Lab and coordination with QMR for QMS 10. Evaluating all methods currently employed by the Lab and for proposing new or modified analytical procedures for potential applications. 11. Solving technical problems related to analytical methods and overseeing the training, quality assurance, safety and competency / proficiency testing practices within the Lab. 12. Being accessible to the unit to provide onsite, telephonic or electronic consultation as needed. 13. Conducting or arranging training for new and existing scientists.14. Conducting performance reviews. 15. Enforcing safety procedures. This duty is shared with laboratory safety officer and safety personnel.

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c. EXPERIENCE AT THE STATE OR NATIONAL OR INTERNATIONAL LEVEL IN HANDLING DEVELOPMENTAL ISSUES OUTSIDE THE UNIVERSITY ENVIRONMENT. INTERNATIONAL EXPERIENCE TO BE PREFERRED

Dr. Idrees has experience in handling developmental issues at the national and international level outside the university environment. Few examples are given below:

1. Dr. Idrees is Visiting Scientist of Centers for Diseases control & Prevention (CDC), US Department of Health & Human Services, Atlanta, USA

2. Dr. Idrees was Member of a team of four Scientist designated by CDC, USA to visit Pakistan (2006-07) to meet with several public health officials from the Ministry of Health and the National Institute of Health to discuss various collaborative public health programs and activities that will support the Field Epidemiology & Laboratory Training Program (FELTP).

3. He is a Member Guidance & Examination Committee of Atta ur Rehman School of Applied Biosciences (previously known as NCVI) National University of Science & Technology (NUST) Islamabad from year 2011 till date. He attended all scientific meeting, Ph. D synopsis/thesis evaluation.

4. His is a Member Board of Studies (BOS) Department of Molecular Biology, Virtual University of Pakistan M. A. Jinnah Campus Lahore and helped in the development of Syllabi and Curriculum for M. Phil in Biotechnology and Microbiology.

5. He was a Member of Expert Committee for the preparation of guidelines of MERS CoV constituted by Advisor to Chief Minister on Health-2014. He actively participated in the preparation of guidelines for this deadly virus for Pakistan.

6. He is Member Cabinet Committee on Dengue Control-Health Department Constituted by Chief Minister, Government of the Punjab and helped in the development of guidelines and SOPs for dengue virus eradication and control.

7. He is also Member (Co-opted) of “Technical Expert Committee of Medicine” recommended by Steering Committee on Prevention & Control of Hepatitis Programme.

8. He is Member/Fellow of eight International Scientific Societies, particularly: Fellow Royal College of Pathologist (FRCPath) London United Kingdom (UK)-Member No. 20005720; American Society of Microbiology (ASM)-Member ID: 56817638; European Society of Clinical Microbiology and Infectious Diseases (ESCMID)- Member ID: 122577; Hellenic Society of Virology; International Society for Antiviral Research ; The Federation of European Microbiological Societies; Zoological Society of Pakistan

9. He is Editor of more than 10 International Scientific journals such as: World Journal of Clinical Infectious Diseases; Global Journal of Gastroentrology & Hepatology; World Journal of Virology; World Journal of Gastroenterology & Hepatology; World Journal of Gastroenterology; The Science; Hepatoma Research; Journal of Hepatitis Research

10. Reviewer of more than 20 International Scientific Journals (few are given below): The Lancet Infectious Diseases; Nature; Emerging Infectious Diseases; Virus Genes; European J. Virological Methods; BMC Virology; BMC Infectious Diseases; Infection, Genetics, Evolution; Journal of Medical Virology

11. Reviewer of grants in the field of Health Biotechnology for HEC of Pakistan. 12. Visiting Scientist of several National & International Institutes/Organizations.

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PART ‘C’ Expected Skills and Competencies

a. Technical Skills ---

i. Openness towards technology and a deep conviction regarding its potential applications in a knowledge-based setting;

Being FRCPath and having vast 19 years experience of working in well-reputed national and international organizations of teaching/research/management at a relatively senior position such as Vice Chancellor, Director CAMB, Group Leader at CEMB, visiting Scientist and designated Member of a team of four Scientist at Center for Diseases Control & Prevention (CDC), US Department of Health & Human Services, Atlanta, USA, Dr. Idress has established various labs, fully equipped with modern and latest equipments at Hazara University, established PCR-based Diagnostic Lab for the detection of infectious diseases at Centre of Excellence in Molecular Biology (CEMB) & Centre for Applied Molecular Biology University of the Punjab wherein and provided general public with reliable, accurate, rapid and more economical diagnostic services for most prevalent infectious and genetic diseases. He has generated a source of income for CAMB and had generated revenue of more than 500 million. As a part of endeavors, to develop indigenous technologies, he has developed Molecular Diagnostic Kits for the qualitative detection of hepatitis B virus, hepatitis C virus, M. tuberculosis, Dengue virus detection & serotyping and HCV Genotyping at 1/10th the cost of the same kits manufactured by other companies in USA. This will help to overcome inherent constraints of practicing modern molecular tools for diseases diagnosis and encourage/contribute to the indigenous technologies in the area of Molecular diagnostics.

ii. Reasonably high level of comfort in the use of technology As Technical Manager, ISO certified Molecular Diagnostic Laboratory (MDL) of CAMB,

member Guidance & Examination Committee at Atta ur Rehman School of Applied Biosciences, National University of Science & Technology (NUST) Islamabad since 2011 till date, as member of Expert Committee for the preparation of guidelines of MERS CoV constituted by Advisor to Chief Minister on Health-2014. Dr. Idrees has actively participated in the preparation of guidelines for deadly virus hepatitis B virus, hepatitis C virus and as a member Cabinet Committee on Dengue Control-Health Department Constituted by Chief Minister, Government of the Punjab helped in the development of guidelines and SOPs for dengue virus eradication and control. He is also Member (Co-opted) of “Technical Expert Committee of Medicine” recommended by Steering Committee on Prevention & Control of Hepatitis Programme.

b. Managerial Skills i. Ability to anticipate issues and problems and prepare advance strategic plans; Being head of the various statutory authorities of a university such as Academic Council, ASRB,

F&P Committee, Selection Board, Syndicate, Senate, Executive Council of Board of Management etc as Vice Chancellor, Director and member of national and international well reputed organizations, Dr. Idrees has strong Managerial Skills and ability to anticipate issues and problems and prepare advance strategic plans with documented experience of restructuring of existing organizations. In year 2015 with the help and cooperation of prominent scientists, he

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has developed an excellent teaching and research programme in Applied Molecular Biology & Forensic Sciences that was approved and launched for the first time in CAMB. In addition, most recently (2018), ten modern and market oriented academic disciplines were approved and launched at Hazara University on just his personal efforts. In addition much awaited construction of the leftover work at Hazara University was started with the financial sponsorship, amounting to Rs. 662 Million, of the Federal Government of Pakistan. Eight magnificent and spacious academic blocks furnished with comfortable furniture and modern equipments for instruction and research are available to the students and faculty.

For this, he had been awarded several awards/honors at national and international level. Last year he has been awarded the most prestigious award “PAS Gold Medal-2017 in Emerging Technologies”, by Pakistan Academy of Sciences. In addition, Dr. Idress also been awarded “TWAS Best Young Scientist of the Year-2009” award, “Research Productivity Awards from 2011 through 2017 for his excellent performance in the field of Molecular Biology. In addition he has also been awarded Certificate of appreciation for excellent lab support in detection of dengue fever cases during last outbreak in the year 2011 and shield for his expert opinion for “Lab Services in Case Detection for Dengue Fever” on February 29, 2012 by WHO.

Dr. Idrees has demonstrable experience of handling Quality issues, assessment and accreditation procedures etc. He is member of National Quality Assurance Committee (NQAC) of Higher Education Commission (HEC). The objectives are to (i) facilitate HEC, Program & Institution Reviewers and University Administration in assessing and adopting the Quality criteria (ii) track the effectiveness of the QA programs of HEC and proposing appropriate modifications (iii) update the Quality Assurance indicators, policies and guidelines of HEC in view of global developments & local stakeholders feedback and proposing Standard Operating Procedures (SoPs) for their adoption (iv) identify future capacity building requirements for institutions to improve the services of institutions towards a growing and more diversified tertiary education sector. In addition he had obtained training on “Quality Management Consultants” containing Internal Auditing, Corrective & Preventive Action conducted by QMC Lahore in year 2009. Further, he was Technical Manager Molecular Diagnostic Laboratory (MDL) of CAMB, Ministry of Science & Technology during its ISO certification & Accreditation.

ii. Ability to generate resources and allocate the same appropriately; Dr. Idrees has all the capabilities of generating resources and its appropriate allocation. He has

already generated a good source of income for CAMB which had generated revenue of more than 500 million; Rs. 662 Million for the much awaited construction of the leftover work at Hazara University with the financial sponsorship of the Federal Government of Pakistan out of which 8 magnificent and spacious academic blocks are furnished with comfortable furniture and modern equipment for instruction and research are made available to the students and faculty.

Prepared and submitted a PC1 for the uplifting of Academic and Infrastructure facilities of Hazara University that was approved at a cost of Rs. 1702 Million by Planning Commission.

iii. Capacity to work effectively under pressure and manage work and resources within tight deadlines;

As head of the University statutory bodies, Dr. Idress has the strength to work effectively without any pressure as he has always keep merit on the top and follow the Act, Statutes, Rules and Regulations in every affair/business of the University.

As a good and friendly team leader, Dr. Idrees has performed every task within the tight deadline with believe in team work.

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iv. Good understanding of financial management including revenue generation, planning and fiscal control

As head of the University Finance & Planning Committee, Dr. Idress has sound knowledge of financial management, revenue generation, planning and fiscal control. He has performed well in the financial management sector, standing Hazara University as most financially secure university of the province. Currently around 13,000 students are enrolled on the campus which is targeted to 30,000 in the next 5 years.

When Dr. Idrees joined Hazara University in July 2016, the budgetary target form own sources for the FY 2016-17 was not achieved and was deficit of 33 Million. Then he took efficient managements by managing different types of funds under various heads and attempted following measures to control Fiscal deficit;

1) Decrease non-plan expenditure 2) Increase revenue: revision of service charges 3) Seek additional budget from the Federal and Provincial Governments 4) Successfully signed more than 20 MoUs including 02 with Army School of Physical

Training and Army Junior Leaders Academy (JLA), Shinkyari Mansehra 12,000 student’s enrollment worth 12 Million per year.

With all these measures and initiatives, the budgetary target form own sources for the FY 2017-18 was successfully achieved with 18 Million above revenue and 15 Million above revenue is expected in the budgetary target form own sources for the FY 2018-19.

c. Alignment with corporate objectives and State as well as National level priorities

i. Ability to identify the needs of the communities in key sectors

We have large population in rural areas which do not get effective higher Education. There is urgent need to increase General Enrolment Rate in higher education by reducing drop outs.

For Education system to produce graduates with desired employment, we need to strategically focus on “Education Modules with Enhanced Employability”. We must design various modules for a subject, which would be adaptable to the students’ aptitude and skills desired. The curricular reforms have to be more need-base, skill-oriented and in alignment with latest developments in technology.

I wish to inculcate innovative culture of ‘Customized Education’ as per needs of an individual (society, market & students) by finding best resources globally and for commercial & industrial development.

As a Vice Chancellor, Dr. Idrees undertook an ambitious project of 20 market oriented academic disciplines which has been approved and established in Hazara University with the above objectives. And for this purpose, he has started and completed the construction of the leftover work at Hazara University with the financial sponsorship, amounting to Rs. 662 Million, of the Federal Government of Pakistan where eight magnificent and spacious academic blocks furnished with comfortable furniture and modern equipments for instruction and research are made available to the students and faculty.

Being well reputed and most citied researcher, Dr. Idrees strongly believe in research to identify needs of the communities. In this regard, he has developed indigenous technologies, Molecular Diagnostic Kits for the qualitative detection of hepatitis B virus, hepatitis C virus, M.

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tuberculosis, Dengue virus detection & serotyping and HCV Genotyping at 1/10th the cost of the same kits manufactured by other companies in USA. This will help to overcome inherent constraints of practicing modern molecular tools for diseases diagnosis and encourage/contribute to the indigenous technologies in the area of Molecular diagnostics.

ii. Deep understanding of the challenges before the nation and how Higher Education can

respond to developmental needs Being a leading Higher Education Institution, first challenge the Hazara University has to face

at national is the shortage of skilled human resources. Thus skill enhancement through Customized education is essential. So, Dr. Idrees has;

a) Tried to improve equality in Higher Education by introducing 10 market oriented academic disciplines in higher education, established well equipped labs and buildings and hired/appointed highly skilled and qualified faculty and staff for that purpose.

b) Introduced new courses and updated the curricula of the entire University through statutory bodies’ regular meetings.

c) Understanding the high education needs of the nation, as a Vice Chancellor, he paid regular visits to various stake holders and hold meetings on variety of topics to get deep understanding of the national challenges and to design successful strategy to overcome.

d) Consequently, Hazara University is now standing in list of leading national higher education institutions as No.3 in the province and No. 17 in the country playing its due role in responding to the nation challenges and developmental needs.

iii. Demonstrable understanding of curriculum development issues, especially those relating

to widening participation and social inclusion As a Vice-Chancellor of Hazara University, Dr. Idrees has taken following initiatives;

a) Introduced competency-based curriculum through the university and its affiliated institutions and hold regular meetings of the statutory bodies for this purpose.

b) Included highly qualified and well know academicians in the list of subject experts. c) Initiated process of establishment after principal approval of statutory bodies of new

market-oriented disciplines like Pharmacy, Public Health, Medical Lab. Technology, Public Administration, Disaster Management, Geology, Physical, Education, Elementary Education, Secondary Education, Environmental Sciences, Biotechnology, Geography (GIS), Molecular Biology, Veterinary (DVM), Forestry, Human Nutrition, Eastern Medicine, Criminology & Forensic Science, of International Relation, History & Civilization.

As a Chairman of Academic Council, he was instrumental in approving all the new market-oriented disciplines with the objectives to respond to the national challenges and developmental needs.

d. Leadership skills i. Exceptional ability to motivate a diverse group of stakeholders

As Vice Chancellor, Dr. Idrees has experienced the need of motivating a diverse group of stakeholders for efficient running of the university. These include students and parents, faculty and administrative staff, support staff, HEC, HED, Provincial and Federal Governments. In this regard, he all the abilities to motivate a diverse group of stakeholders wherein he has initiated;

a) Student centric activities as elaborated elsewhere

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b) Timely communication with parents of students c) Encouraging teachers for conducting quality research, attending

workshops/seminars/conferences by providing incentives d) Frequent interactions/meetings with administrative staff, Government incentives like

additional increments etc. e) Generating funds from Corporate sector / Government f) Getting timely help of stake holders and Social organizations g) Active participation in improvement of Hazara University

All the stakeholders have been motivated to work closely with us for the benefit of the University.

During his tenure as Vice Chancellor, administrative affairs of the University are being run in strict compliance with the provisions of the Khyber Pakhtunkhwa Universities Act 2012 (Amended 2016). The Hazara University Statutes 2016 have been framed by a team of experts; comprised of the faculty and administration. These statutes were approved by the Senate of University well in the stipulated time. The same are notified by the Higher Education Department, Government of Khyber Pakhtunkhwa on April 28, 2016. Hazara University is one of the first two universities of the province who have approved service statutes in the stipulated period. All authorities and statutory bodies of the University are complete and regularly convened to effectively govern the University.

To bring transparency in the system, automation of all presently performed manual processes has been geared and every ongoing activity in the domain of admission and recruitment is shared with the stakeholders through web page of the University. Hazara University has a very pleasant working relationship with the all the stake holders including Higher Education Department (HED) Government of Khyber Pakhtunkhwa, Higher Education Commission (HEC) of Pakistan Islamabad, District and Divisional Managements of District Mansehra and Hazara Division.

ii. Keen desire to further the mission and goals of the organization Because of his keen interest in academics, Dr. Idrees undertook fulltime teaching and

administrative job in various government organizations like, MoST, University of the Punjab and Hazara University. So far he has attained 19 years experience of teaching, research and administration in various capacities. During his career he has seen closely the needs in higher education at national level. Thus he has developed a dream to serve at university level to fulfill these needs for the nation. This keen interest has prompted him to apply for this post of Vice Chancellor.

a) Main purpose of higher education institutes is to offer better quality education to all human kind. They need to be healthy for better development of creative talent and critical thinking.

b) Creation and maintenance higher education is goal to achieve. Educated people create wealth and in turn prosper the nation.

c) Provision of quality education at the door step and its improvement is a dream that needs to come true.

d) Getting quality education has become costly and university needs to exert to see that it comes within reach of poor.

e) Educational goals: Hazara University stands 2nd in the province and 27 in the country. f) Administrative goals: efficient management of fiscal provisions as elaborated elsewhere.

iii. Ability to think strategically and innovatively and maintain a broad perspective

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Since his schooling, Dr. Idrees has inquisitive mind. He always been thinking strategically and innovatively with a broad perspective. After his post graduation degree though being in natural science, he undertook PhD work on innovative topic of Molecular Biology with specialization in Molecular Bacteriology/ Molecular Virology and with innovative methodology and completed it successfully. This spirit reflects in my academic career so far. His research work, innovations and guidance to the student in developing new equipment and patenting are indicative of this spirit.

Right from the first day he took charge as Vice Chancellor, Hazara University, he has been working with strategy of future development. Milestones like establishment of new market oriented discipline/departments, procurement of many types of equipment for them, planning, start and completion of construction of 8 new academic blocks are indicative of the broadened perspective of the strategy he decided.

Administrative reforms throughout the University.

Installation and implementation of Biometric Based Attendance System

iv. Ability to lead by personal example with openness to new ideas and a consultative approach in implementation of the same Dr. Idress has good reputation of established researcher with highly referred and citation

data on Google Scholar. He can carry research on variety of topics in a goal oriented manner. This includes all Institutional research based functions from labs, training activities, student, trainer, parent and stakeholder feedback. Technology based research can be used as a backbone of Institutional activities along with modern educational innovations /strategic interventions.

He can order any higher education professional and researcher to create necessary infrastructure needed for higher education and for benefit of students including and faculty.

He has developed Molecular Diagnostic Kits for the qualitative detection of hepatitis B virus, hepatitis C virus, M. tuberculosis, Dengue virus detection & serotyping and HCV Genotyping at 1/10th the cost of the same kits manufactured by other companies in USA. This will help to overcome inherent constraints of practicing modern molecular tools for diseases diagnosis and encourage/contribute to the indigenous technologies in the area of Molecular diagnostics.

He was member of Expert Committee for the preparation of guidelines of MERS CoV constituted by Advisor to Chief Minister on Health-2014. Dr. Idrees has actively participated in the preparation of guidelines for deadly virus hepatitis B virus, hepatitis C virus and as a member Cabinet Committee on Dengue Control-Health Department Constituted by Chief Minister, Government of the Punjab helped in the development of guidelines and SOPs for dengue virus eradication and control.

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e. Interpersonal communication and collaborative skills

i. Demonstrable success in developing and executing National and International

collaborative arrangements

All with his visionery and futurstic mission, Dr. Idress has developed a projecte namely “Goals for 2030”. Under this project the University is committed & devoted to build a core of idea of exchange of knowledge for services to the community in nourishing students, faculty, staff members and ultimately the whole nation.

i. Under his leadership, the Hazara University is striving to create a collaborative environment open to the free exchange of ideas, where research, creativity, innovation and entrepreneurship can flourish by attracting and retaining diverse world class talent and ensuring that every individual can achieve all his potential. Strategic Plan (Vision 2030) has been approved and university is being steering in the directions set there in. In 2030 University targetted to have 90 % PhD faculty and by 2035 100 % PhD faclty. The University will attract interest of foreign qualified faculty. The University will manage open access interaction between world class foreign researchers and Hazara University researchers/scholars through web conferencing. In 2030, Hazara University will have international research exchange center and the conferences will be planned on monthly basis. Under the 2030 project, the University planned to transfer by 2030 the conventional teaching methodology with latest electronic teaching methodology with visual aids and will have best international standard infrastructure facilities for students, researchers, and all other stakeholders including Academic Departments, Labs, IT Labs, libraries, E-Libraries, Boarding/Lodging, Accommodation, Fitness Clubs, Hospital etc.

ii. In this regard, 07 MoUs with International Organizations and 14 MoUs with national

organizations have been inked for collaboration in academic and research activities and students exchange through Office of Research Innovation and Commercialization (ORIC) which is fully functioning in the University. While under the 2030 vision, the university plans to sign MoUs for collaboration in applied research with 200 Natinal and 100 International Universities/industries/professional organisations.

ii. Ability to interact effectively and persuasively with a strong knowledge-base at senior levels and in large forums as well as on a one-to-one basis

Prof. Dr. Mohammad Idrees has the required abilities to interact effectively and persuasively with a strong knowledge-base at senior levels and in large forums as well as on a one-on-one basis as in the capacities given on pages 56-58.

iii. Evidence of being an active member of Professional Bodies and Associations in pertinent Field

Pages nos. 74-75.

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KEY ACHIEVEMENTS OF DR. IDREES, AS VICE CHANCELLOR OF HAZARA UNIVERSITY DURING THE PERIOD (28-07-2016 to 28-07-2019): After assuming charge of permanent Vice Chancellor, Hazara University on July 28, 2016, he has focused on the development, modernization and progress of the University with following key achievements during his tenure:

A. Administration:

1. Implementation of Khyber Pakhtunkhwa Universities Act 2012 (Amended 2016) in true letter and spirit.

2. Framing and approval of the Hazara University Statutes 2016 in the stipulated period. 3. Completion of all Authorities & Statutory bodies. 4. Strict compliance with the University Calendar (admission, teaching, exams, convocations. 5. Automation of all process of recruitment & Admission 6. Approval of Strategic plan (2030) by Senate 7. Convening of promotion/selection boards for administrative officers & faculty; enhancement

of transport facility; launching of ten modern academic disciplines; and Campus beatification. 8. During 2016-2019, 61 meetings of the statutory bodies including Senate, Syndicate, Academic

Council, Advanced Studies & Research Board, Finance & Planning Committee and Affiliation Committee have been conducted successfully which is unprecedented in the history of HU.

9. During 2016-2019 SEVEN meetings of the Selection Boards have been called for appointment of the Faculty members and promotions/appointments of the Administrative Officers ensuring merit and transparency.

10. Central Biometric Attendance System has been installed and fully functioning. Achieved dramatic increase in Faculty & staff attendance.

B. Academic

1. Ten modern and market oriented academic disciplines were approved and launched in HU. 2. Several degree programs such as Agriculture, IT and architecture engineering were

accredited during my tenure. 3. Students enrollment were increased by 45% (from 7000 to 13000). 4. Scholarships of more than 64 million were granted to more than 1800 students 5. Student : teacher ratio were maintained as per HEC requirements. 6. Besides digital library, 72000 books were purched for library. 7. Established its Batagram Campus under HEC project and started academic functioning

from the Fall Semester 2018 8. According to the recent “Top Universities in Pakistan, 2019-Pakistani University Ranking

report (2019) (https://www.4icu.org/pk/) by uniRank Tuition Range Matrix™, a non-academic League Table of the top Pakistani Universities based on valid, unbiased and non-influenceable web metrics provided by independent web intelligence source, the ranking of

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Hazara University in general universities has elevated in ‘Top University Ranking’ in province, nationally and internationally (from 12th to 3rd top university at Provincial level and from 64th to 17th top university at National level).

C. Research 1. The achievement of research conducted in the university has been unparalleled; the faculty

of the university has registered one patent, introduced a new and more productive variety of brassica seeds,

2. Published more than 1000 research papers in the leading research journals, 3. Produced more than 100 PhD Scholars, graduated more than 1500 M. Phil scholars, 4. Organized a series of national and international conferences, conducted and participated in

more than 100 research seminars & workshops. 5. For ensuring the food security in the country, new plant-type of Rice has been developed

by Prof. Dr. Fida Abbasi. It grows 6 to 8 feet tall with record yield of 15 tons per hectare. Granted patent by USA & is the first crop in the 71-years history of Pakistan granted with US patent.

6. Office of Research Innovation and Commercialization (ORIC) has become fully functional in the University. The office has signed 06 MoUs with International Organizations and 14 MoUs with national organizations for collaboration in academic and research activities and students exchange. In addition last year we have inked MOUs with two Army Schools/Institutions (JLA Shinkyari & ASPT Kakul) for their academic activities (generated income120 million/year).

7. The University recorded resounding success in the area of fellowships and collaborations such as: recorded an increase in the number of Staff winning fellowships; one faculty received Gallino-Denardo Award by International Commission of Optics and Internarial Center for Theoretical Physics, Italy in the field of Laser Technology; Higher Education Department, Khyber Pakhtunkhwa awarded Rs. 3.2 million project to Dr. Jehan Akbar for establishing Pakistan’s 1st and (3rd in the world) Spectroscopy Laboratory at Hazara University Mansehra; Our faculty members were appointed as member Think Tank for Tourism KPK by Tourism Department KP. Received the most prestigious award: PAS Gold Medal in Emerging Technologies”, by Pakistan Academy of Sciences.

8. The QEC completed the tasks required by the HEC with regard to conducting Self Institutional Performance Evaluation S-IPE. Our QEC attained membership of International Network for Quality Assurance Agency in Higher Education (INQAAHE) this year.

9. Training Center for training in different areas 10. ORIC was established and is now fully functional 11. Strengthened QEC and its performance wa quantified by HEC.

D. Financial Management

1. The financial status of the University became very sound. Two budgets out of the last three budgets were surplus and third budget was no deficit budget. Financial Assets have been managed very well. Pension fund and Staff welfare funds have been judiciously invested at very high rates in the current prevailed financial scenarios.

2. This year, University has initiated a process to establish transparent Examination System. Two folded approach is being followed. Under this approach all processes of the

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examination system will be automated and all rules and regulation will be updated to ensure the transparency of the examination systems in next 6 months.

3. An amount of Rs. 512 million were approved for Hazara University Faculty Development Program by Planning Commission of Pakistan under HEC Umbrella PC1. In addition, University has faculty development program amounting to more than Rs.50 Millions in the mega project which is expected to be launched in 2019-20.

E. Security & Law and Order of the University

1. Law and Order situation in Hazara University during last three years has been exemplary. All semesters have been commenced and ended as per academic cylinder of the University. No unrest among students or staff or faculty was recorded. All educational, sports, cultural and recreational activities were held without any fear of inner or outer threat.

2. The University has implemented a huge CCTV network comprised of more than 90 high tech cameras. The CCTV surveillance system is equipped with latest equipment connected via optical fiber having video storage capabilities of 30 days and 24/7(99.9%) up time. The system also has emergency siren and communication device for quick response to any security breach. Law and Order situation in Hazara University during last three years has been satisfactory. All semesters have been commenced and ended as per academic calendar of the University. No unrest among students or staff or faculty was recorded.

3. University is making every effort to uphold slogan of clean and green Pakistan. Over last year’s more than 20,000 plants and flowers have been planted in the University. This has changed the landscape of the University.

F. Funding details:

1. Financial status of the University become very sound 2. All the budgets were surplus 3. Financial assets have been managed very well 4. Pension fund and staff welfare funds have been invested at very high rates.

G. Infrastructure

11. Completion of leftover work of the Turkish NGO by constructing 8 Academic Blocks at HU with the financial sponsorship of HEC, amounting to Rs. 662 Million, (covered area of 4’00’000 Sft), furnished with comfortable furniture and modern equipment for instruction and research.

12. Constructed two faculty hostels (one each for male & female faculty) with the financial sponsorship, amounting to Rs. 65.924 Million, of the HED/Provincial Government.

13. Completed Smart University Project (sponsored by HEC) just in 6 months record time and now Wi-Fi carpet coverage across the campus is provided with high speed bandwidth of 424 MB. Researchers are now able to access all digital resources at a very high speed anywhere in the campus. In next 1 year the facility will be extended to SAFE Campus and SMART Classroom.

14. A mega project for the uplifting of Academic and Infrastructure facilities of Hazara University has recently been approved at a cost of Rs. 1702 Million by Planning Commission of Pakistan.

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