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University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic COST 863 Berryfruit Virus Small Group Josef Špak

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Page 1: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

University of South Bohemia České Budějovice

Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

COST 863 Berryfruit Virus Small Group

Josef Špak

Page 2: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

COST 863 Berryfruit Virus Small Group

• Since the meeting in Antalya we were discussing with Bob Martin (Corvallis USA), Willi Jelkmann, (BBA, Germany) and Stuart MacFarlane (SCRI, UK) a format for cooperation of scientists working on virus and virus-like diseases of small fruits/berries.

• COST 863 Action WG 2, which includes also pathogens might be good forum for this purpose.

• So far I was the only member giving talks about virus-like diseases. Most of pathogen-related talks were given on fungi/bacteria!!!

Page 3: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Vývoj diagnostiky virů v ÚMBR AV ČR• Strawberry vein banding virus (SVBV)• Karanténní pro Evropu• Nejsou protilátky• Neznámý genom

Příznaky virové infekce v jahodníku

Částice viru v elektronovém mikroskopu

Page 4: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Vývoj diagnostiky SVBV

• První kompletní sekvence viru

• Organizace genomu viru

• Diagnostika viru

PETRZIK, K., BENEŠ, V., MRÁZ, I., HONETŠLEGROVÁ-FRÁNOVÁ, J., ANSORGE, W., ŠPAK, J.: Virus Genes 16: 303 - 305, 1998.

Page 5: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Vývoj molekulární diagnostiky SVBVProjekt EU 5th Framework „Virus Detector“ 2000-2003

Soubor izolátů a sekvencí SVBV a příbuzných virů

Page 6: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Vývoj molekulární diagnostiky SVBVProjekt EU „Virus Detector“ 2000-2003

Vašková D, Špak J, Klerks MM, Schoen CD, Thompson JR, Jelkmann W: Real-time NASBA for detection of Strawberry vein banding virus Eur. J. Plant Pathol. 110 213-221 2004

- návrh primerů pro PCR- ověření detekce viru v

polních vzorcích

Page 7: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

COST 863 Berryfruit Virus Small Group

• Idea 1 Establish the COST 863 Berryfruit Virus Small Group within WG 2

• Idea 2 Prepare a Small Group meeting (SG) attached to some conference

• Qualification to attend– Active research on berryfruit virus/phytoplasma– Talk (preferably)/poster

Page 8: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

COST 863 Berryfruit Virus Small Group

Tentative programme of the SG:• Talks of SG members• Talks of 2 invited speakers including non EU

member states (U.S., Israel etc.) • Discussion current research topics

– RBDV resistance breaking in raspberry– RBDV in grapevines– BRV – severe/mild isolates etc.

• Research cooperation• Joint projects in FP 7

Page 9: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic
Page 10: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic
Page 11: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic
Page 12: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic
Page 13: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Berryfruit Virus SG Meeting

• IOBC Workshop on Integrated Soft Fruit production, organized at East Malling on 24-27 September 2007

• Very much complementary with the scope of COST 863, I suppose this could be a good occasion for the SG berryfruit virology meeting.

• East Malling is a traditional place for small fruits

• Preliminary agreement with the local organizer Jerry Cross (room, data projector, fee?)

• SG could start on Thursday afternoon and continue on Friday, September 28th 2007

• The SG participants could be reimbursed from COST 863 travel, accommodation and subsistence for 2 days?

Page 14: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

• Letter to potential SG members – March 2007

• Please, let me know your opinion: • Are you interested to establish and participate in a

berry virus small group within the WG 2 of COST 863?• Are you interested to attend the SG meeting attached

to the IOBC Workshop on Integrated Soft Fruit production, organized at East Malling on 24-27 September 2007, when I will be able to get financial support for travel reimbursement from COST 863 Action?

• Response statistics

COST 863 Berryfruit Virus Small Group

Page 15: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Berryfruit Virus Small Group

COST 863 Beryfruit Small Group Statistics

Name Town Country SG Interest IOBC 2007 Martin R.R. Corvallis USA + + Tzanetzakis I. Corvallis USA + + Jelkmann W. Dossenheim Germany + + Lemmetty A. Jokioinen Finland + No res. funds Lehto K. Turku Finland - - Cieslinśka M. Skierniewice Poland + + Malinowski T. Skierniewice Poland + - MacFarlane S. Dundee UK ? ? Nyergyes K. Velence Hungary + + Paunovic S. Cacak Serbia - - Isac V. Maracineni Romania + + Spak J C. Budejovice Czech Rep. + + Bourrain L. Bellegarde France ? ? Mavrič-Pleško I. Ljubljana Slovenia + - Total 10 7

Page 16: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

• Do not hesitate to inform about this activity anyone in berryfruit virus/phytoplasma research field.

COST 863 Berryfruit Virus Small Group

Page 17: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

• Points to discuss

• SG establishment Yes/No

• SG meeting at East Malling Yes/No

• Programme amendments

• Participants (up to 10 pers.?)

• Budget

COST 863 Berryfruit Virus Small Group

Page 18: University of South Bohemia České Budějovice Institute of Plant Molecular Biology Biology Centre, Academy of Sciences České Budějovice, Czech Republic

Thank your for your attention