unit 19 - biotechnology
TRANSCRIPT
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Biotechnology
To accompany Georgia Middle School Lessons on Biotechnology
Written by: Christina M. Spears
Georgia Agriculture Education Curriculum Office
July 2003
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Objectives
• Define Biotechnology• Compare and contrast biotechnology and
genetic engineering• Explain how biotechnology helps increase
production• List uses of biotechnology in plant and
animal science• Explain historical uses of biotechnology
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What is DNA?
• Hereditary information stored in chromosomes
• Stored as sequence of nucleotides
• Five carbon base sugar
• Phosphate group
• Nitrogenous base(c)
http://whyfiles.org/075genome/images/dna.gif
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What is Biotechnology?
• Management of biological systems for benefit of humanity
• Applied to general sciences
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Uses of Biotechnology in Plant and Animal Science
• Cloning• Herbicides• Increasing shelf
life
© http://www.amnh.org/exhibitions/genomics/5_reshaping/images/dolly.jpg
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Biotechnology vs Genetic Engineering
Biotechnology• All subjects of science• Bigger picture
Genetic Engineering• Genetics• Gene splicing,
replication, and transfer of genes
• More concentrated
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How does Biotechnology increase Production?
• Develop new forms of plants and animals
• Modify plants and animals to improve their life processes
• Improving the quality of food
• Longer shelf life for perishables
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Historical Uses of Biotechnology
• Earliest techniques were used to make cheese and wine
• Spoiled milk fermented to make cheese
• Grape juice fermented to make wine
• Today…we not only see wine and cheese but also yogurt and bread made from processes of biotechnology
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Activity
• THE OLD RED DOG WAS TOO BIG FOR HIS BED
• Have a deletion that would delete the D in OLD…sentence look like
• THE OLR EDD OGW AST OOB IGF ORH ISB ED
• If mutation caused inversion of word DOG…• THE OLD RED GOD WAS TOO BIG FOR HIS
BED
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Intro to BiotechnologyLab Activity!!
• Making Yogurt
Review handout on it
Form groups of three people and work together to make the yogurt
Two-day activity
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Biotechnology
Biotechnology in Plant Science
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Objectives
• Identify biotechnologies in plant science
• Discuss importance of biotechnology to humans
• Explain the impact of cloning on plant science
• Identify how manipulation of chromosome number affects plant characteristics
(c) http://www.myjanee.com/images/photoalbum/flora/marigolds.jpg
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Assignment
• In groups of three research one plant biotechnology
• Hybrids
• Herbicides
• Genetic engineering
• Number of chromosomes
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Discussion
• What are some biotechnologies used in plant science?
• How will these biotechnologies help plants?• Identify some negative impacts of
biotechnology use in plants• What is the danger of using herbicides?• How can grain be produced more efficiently
with the use of nitrogen?
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Number of Chromosomes• What is polyploidy?
• Contain more than one haploid set of chromosomes (n)
• How does polyploidy affect plants?
• About 30-70% of angiosperms are polyploids
• Peanut is 4n, Banana and apple 2n or 3n, and cotton is 4n
• Deliberate polyploidy in marigolds, snapdragons, and watermelons
(c) http://www.mahyco.com/images/cotton.jpg
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Lab Assignment
• Asexual Propagation:• Cuttings
• Layerings
• For assignment:• Students break up into lab groups
• Each group given a plant for stem tip cutting
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Biotechnology
Biotechnology
In Animal Science
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Objectives
• Identify examples of biotechnology in animal science
• Interpret magazine/newspaper and Internet articles about biotechnology
• Describe how cloning affects animal science
• Explain how the number of chromosomes influences animal characteristics
(c) http://www.advancedcell.com/pg_page1.html
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Assignment
• In groups of three you will research a topic on biotechnology in animal science
• After your research, your group will present your topic to the class
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Discussion
• What are some biotechnologies used in animal science?
• How will these biotechnologies help in the productivity of animals?
• What are the impacts of these biotechnologies on the environment, consumer health, and animals?
• How will these biotechnologies benefit humans?• What is cloning and how will it impact animal
science?
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Epistasis
• Define epistasis.• Having one or more genes
that mask the effect of the dominant gene
• Example: In Labrador Retrievers, BB/Bb gives black, bb gives brown, EE/Ee gives normal coat color, and ee gives yellow.
Cross BBEE with bbEE, what is the F1 generation?
Now cross the F1 generation, what is the F2?
© www.belquest.com/ homelogo02.jpg
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Polyploidy
• What is polyploidy and what kinds of animals are polyploids?
• Polyploidy means having one or more set of haploid chromosomes (n).
• In animals this phenomenon is rare, but one does see it in reptiles, amphibians, and insects
• In the September 1999 issue of Nature, scientists revealed they found a tetraploid (4n) rat in Argentina
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Biotechnology
Genetic Engineering
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Objectives
• Explain genetic engineering• Describe a procedure of
genetic engineering• Identify the process of DNA
fingerprinting• Complete the DNA
fingerprinting lab• Explain how genetic
engineering is applied in agriculture
• Cite examples of genetic engineering uses in agriculture
© http://www.sierraclub.org/biotech/report.asp
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Genetic Engineering
• What is genetic engineering?• Also called recombinant DNA technology or gene
manipulation. Two major techniques: 1) Genes are engineered into organisms such as microbes, bacteria, for mass-production and 2) insertion of select genes into organisms to improve their genotype
• Identify a procedure of genetic engineering.• 1) Isolate the genes, 2) Recombination, 3)
Transformation, 4) Screening, and 5) Induction
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Gel Electrophoresis
• Uses an electric current to separate various fragments of DNA to different lengths on the gel.
• DNA fragments are attracted to the positive side of the gel
• The larger fragments migrate slower than the smaller fragments
(c) http://medlib.med.utah.edu/block2/biochem/Formosa/Figures/Lecture6/6-01%20Agarose%20gel.GIF
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Genetic Engineering in Agriculture
• How is genetic engineering
applied to agriculture?• Crops have been developed that
resist insect damage and spoilage. Some species of plants have the ability to kill off weeds surrounding them. Scientists now insert a gene to prolong the shelf life of fruits and vegetables.
• What are some uses of genetic engineering in agriculture?
• Black walnut has ability to kill off surrounding weeds in its area. Once this gene is isolated, scientists can apply it to other plants. Tomato has a gene to increase its shelf life.
(c) http://www.isu.edu/pics/treewalk/black-walnut1.gif