unc biomolecular x-ray crystallography facility
TRANSCRIPT
X-Ray Safety Course:
UNC Biomolecular X-ray Crystallography Facility
Before you enter the X-ray lab, put your body and ring badge on,
EVEN IF THE X-RAYS ARE NOT ON. Badges are kept in a box,
appropriately labeled “BADGES” outside the lab
Do NOT enter the X-ray lab, if the Safety Interlock Keypad is
in Bypass mode, indicated by a RED light in the upper left
corner, as shown below. A flashing red light on the
generator tabletop and an audible alarm also indicate Bypass
mode.
You should also see a sign posted on the door if any of the
safety devices have been disabled, another indication to not
enter the lab until checking with the Facility Manager
If the computer screen for the detector indicates that someone
else is using the instrument, again, DO NOT ENTER the
LAB
It is only appropriate to enter the lab if the Safety Interlock is in On mode,
as indicated by a GREEN light in upper right corner of the keypad. In this
mode, if any of the doors into the lab are opened, BOTH shutters will
close immediately
Door closed Door open
Once you open the door when the keypad is in safety mode, the
shutter will not open until you CLOSE THE DOOR and then punch in
the correct code (you will receive the code when you are trained)
Once you are in the lab, check whether the generator is
running at Full power - on the Rigaku Micromax 007HF this
is Tube Voltage = 40 kW, Tube current = 30 mA. On the
older RU-H3R, it is 50 kW, 100 mA. If it is NOT, ask the
Facility Manager to help you
The X-ray On indicator light is a RED light, displayed outside
the door to show that X-rays are being produced, NOT
necessarily that the shutter is open and X-rays are coming out
onto the sample.
The shutter open indicator lights are yellow, located inside
the lab near the center of the generator tabletop, there is one
for the LEFT port (CCD detector and Actor) and one for the
RIGHT port (R-Axis IV++)
Light off, shutter closed Light on, shutter open, RIGHT port
If you have not entered the lab with the safety interlock in BYPASS,
You should never see the shutter lights ON when you are inside the lab
When you mount your crystal by hand, you should know
where the direct beam is, even though the shutter should
NEVER be open when you are mounting your crystal
NEVER EVER PUT YOUR FINGERS (or any other
part of your body) IN THE PATH OF THE DIRECT
BEAM (RED ARROW) IF THE SHUTTER IS OPEN
Now, HAPPY STRUCTURE SOLVING!!!
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.