Ultracentrifuges

Sales and Support in the US and CA by:

NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | 763.553.1270 | WWW.NUAIRE.COM/HITACHI

These general-purpose ultracentrifuges have the highest performance in their class* — 100,000 rpm (CP100WX) — making them ideal for the wide range of separation tasks used in cell biology, biochemistry and ano-material fields. For reliability and efficiency to match their speed and quiet operation, they incorporate automatic rotor-life management (RLM), a large color LCD display and positive-feedback (click-type) touch pad entry. Other standard features include RCF (x g) computation and display functions and real-time control (RTC) for direct entry of start/stop times and other parameters. A log options list permits connection with a PC (for simulations and logging of rotor and centrifuge operation histories), printer connection, user security functions and more. *Versus other similar ultracentrifuges in this class, as of October 2012.

1

A New Era of Rotor Life Management

Drive unit warranted for 10 years

0° is maintained by electronic coolingAn electronic cooling system, based on a thermo-module maintains samples at 0°, even at maximum acceleration (ambient temperatures to 30°).

Energy savingsPower consumption is reduced by 10% (in-house comparison) through the use of an IGBT inverter.

Exceptionally low noise – 53 dB or less

Based on EU requirements for mechanical and electrical safety and electromagnetic compatibility

Brake control system for reverting motor electric power to the power source sideHeating during deceleration is thus curbed.

Environment friendly productAbout 80% of the centrifuge proper is composed of recyclable materials.

Advanced technology

Operation records of each rotor are stored in the RLM (Rotor Life Management) memory and the RLM element embedded in the rotor, eliminating need for manual rotor logbook entries. (Naturally, non-RLM rotors still require manual logging.)

Rotor life is automatically managed

Automatic rotor life management system records rotor type, serial number, total number of runs and accumulated run time of the rotor. By precise automatic tracking of the rpm profile and operation time to the nearest 0.1 hours and 0.1 times means, the rotor life is extended as much as 10 times longer than as conventional calculation.

Rotor life is automatically extended

Samples need to be balanced within 5 mm by visual check only. Non-contact imbalance sensor always monitors vibration of the rotor and drive shaft. In case of abnormal vibration, the sensor activates and stops operation immediately.

Powerful imbalance protectionThe highly efficient and compact drive unit can reach maximum acceleration of 100,000 rpm in 5 minutes (CP-100WX). The motor spins in a vacuum, with a unique wire-suspension design.

*Versus other similar ultracentrifuges in this class, as of October 2012.

The highest acceleration and RCF100,000 rpm and RCF to 803,000 x g*

Safety and environmental impact reduction

2Ultracentrifuges

The liquid crystal screen simultaneously displays set values and the actual operating conditions.

Microcomputer control functions

Easy operation

These functions can be selected through the interactive screen display.

• Setting of date • Setting of time • Print-out function (optional) • User time reservations • User registration • Screen contrast adjustment • Setting of the zonal rotation speed • Identification number of the

(2,000 – 3,000 rpm) centrifuge unit

Powerful customization functions

The operating height of the work top was lowered to 33½ in (85 cm), making the large-sized rotors easier to load and unload.

Low table height easier rotor handling

Operators input desired start times directly and can input both start time and operating profile in advance for unattended operation.

Real-time control (RTC) simplifies timer settings

When sharing a centrifuge under joint utilization or in RI facilities, the optional lockout system limits utilization to registered personnel only.

User lockout

Keypad input is enhanced by gentle “click” feedback to confirm correct entry. Operation errors or faults are immediately indicated by means of an alarm display.

Color liquid crystal display and touch-sensitive panel

The user name can be shown through ID code input. This function allows interactive verification of users.

RCF max. and RCF avg. at the selected rotational speed are automatically calculated and displayed for a given rotor, reducing bothersome calculations. The rpm and operating profile are calculated simply by setting the desired RCF.

Operating parameters are automatically computed and displayed, based on selected RCF

In order to prevent from exhausting bio-hazardous sample to a room, a HEPA filter can be assembled in a vacuum line at option.

Biosafety option

User name is shown on screen, linked to user ID code

3

Data communicationOptional LAN board is available. You can link CP-WX series to your PC through ethernet or internet. Optional software “himac LogManager Ver. 3.1 (network edition)” can be installed in your PC and operating history and data of CP-WX series can be easily managed by your PC.

himac LogManager Ver. 3.1 for Windows® (Network Edition)himac LogManager Ver. 3.1 for Windows® (Network Edition) is useful and convenient software to manage real time operating log of himac CP-WX series, CS150NX, C150FNX, CS22N and CR-GIII series. Maximum 16 units of the abovementioned centrifuges can be registered to the software. As data communication between the centrifuge and PC is done via LAN or Ethernet, so there is no distance limitation to establish a network. You can easily establish the network configuration and relocation with commercially available LAN devices. (Optional LAN board is required for the centrifuges.) It means you can manage the operating log at the different location from the installation site of the centrifuges.

This software is real-time log management software; recording period is selectable from 10 seconds to 5 minutes interval. Of course, the software supports U.S. FDA 21 CFR Part 11.

The following functions are available:

• Digital Signature• Audit Trail• Encrypted Data Files etc.

Compatible OS:

Windows® XP, Windows Vista®, Windows® 7 Professional

Windows®, Windows® XP, Windows Vista® and Windows® 7 are registered trademarks of Microsoft Corporation in the United States and other countries.

- Optional log management software

himac ASSISThimac ASSIST is simulation and calculation software of centrifugal condition and can be installed in your Windows®-based PC. You can simulate whether the centrifugal condition is appropriate before the centrifugation, also can simulate optimal centrifugal condition of a sample, whose centrifugal condition is unknown, based on your ultracentrifuge and rotor.

himac ASSIST has following functions:

• Calculations of K factor and pelleting time• Calculation of the allowable rpm with high-density liquid• Rate zonal simulation• Isopycnic simulation• Solvent concentration conversion• Mutual conversion of molecular parameters• Rotor database

- Optional centrifugation simulation software

4Ultracentrifuges

himac original “self-locking rotor system” is the most simple system to set the rotor onto the drive shaft. You just simply place the rotor on the drive shaft and that’s all. The rotor is locked by centrifugal force automatically. You do not need to fix the rotor by screw or push the button to lock or unlock the rotor like other models available in the market.

Self-locking rotor system

Rotors

himac original S-cap series offer simple and easy operation of the tube cap. This unique system requires just three components and three steps to assemble the cap to the tube.

• No consumables, such as O-ring • Easy cleaning and no maintenanceThree assembling steps:

1. Fill sample into the tube up to 80% volume and insert AL Stem into the tube by the tool.

2. Assemble Ti Ring on the tube and tighten AL Stem and Ti Ring by the tool.

3. Fill up the sample into the tube by an injector and close Setscrew.

S-cap series

P32ZT: Zonal centrifugation systemIn zonal centrifugation, a density gradient is developed within the zonal rotor running at low speed. Next, the samples to be separated are loaded through the center of the zonal rotor and are separated at a preset high speed. After separation, the gradient is unloaded and collected through the center by injecting high-density fluid from the outside wall of the zonal rotor while the rotor is running. The desired fractions are moved into the fraction collector via a spectrophotometer using a flow cell which monitors and records the optical density of the fractions.

P32ZTZonal rotorPart No. 9023160M

P32CT: Continuous flow centrifugation systemThe Model P32CT Continuous Flow Rotor, designed for use with Hitachi preparative ultracentrifuges, enables you to perform highly efficient continuous flow separation and purification of large volumes under high centrifugal force.

Product made to special specification

P32CTContinuous flow rotorPart No. 9022660M

940 ML core ass'y. Part No. 348333A

For separating samples containing much sediment or performing separation after increasing the density gradient, the 940 ml core ass'y increases the rotor capacity to 940 ml. The flow volume performance is about 35% less than that of standard cores.

Configuration of P32CT System flow diagram

Separation Procedure

5

Rotor list

Type Model Part No.(automatic)Max.

speed(rpm)

Max. RCF(x g)

Nominalcapacity

(ml xnumber ofsamples)

K-factor Main

purposeMain purpose

Fixed angleP100AT2

P100AT2* 9023113M 100,000 803,000 6.5 x 8 18 Separation of microscopic particles and lipoprotein

P90AT* 9023053M 90,000 700,000 12 x 8 25Separation of cell organelles, Plasmid DNA and RNA Rapid separation of intermediate volume sampleP80AT

* 9023093M 80,000 615,000 12 x 8 27

P70AT2* 9022753M 70,000 452,000 12 x 12 36

P70AT* 9022623M 70,000 505,000 40 x 8 44 Separation of Norovirus in food productsRapid separation of intermediate-to-large volume sample

P65A* 9022313M 65,000 370,000 12 x 10 48 Separation of cell organelles, Plasmid DNA and RNAHigh-speed separation of intermediate volume sample

P50AT4 9024111M 50,000 316,000(outside tube cavity)

6.5 x 44 32(outside tube cavity)

Separation of lipoprotein

P50AT2* 9022633M 50,000 303,000 40 x 12 70 Separation of Norovirus in food productsRapid separation of intermediate-to-large volume sample

P50A3 9023140M 50,000 252,000 1.5 x 24 33 Rapid pelletting micro volume samples

P45AT* 9022643M 45,000 235,000 94 x 6 130 Rapid separation of large volume samples

P42AT 9022651M 42,000 223,000 0.23 x 72 12 Rapid separation of lipoprotein for examination(for micro volume polyspecimen)

P32AT* 9023150M 32,000 111,000 12 x 32 186 Simultaneous processing of polyspecimen

P27A 9023121M 27,000 106,000 160 x 6 352 Separation of large volume samples (pretreatment for manufacturing vaccines)

P19A 9022221M 19,000 55,100 230 x 6 754 Separation of large volume samples

NeoangleP90NT

P90NT* 9023073M 90,000 646,000 5 x 8 10Rapid separation of Plasmid DNA and RNA

P65NT* 9024153M 65,000 402,000 12 x 10 23

P65NT2* 9023103M 65,000 431,000 5 x 18 15 Processing of many tubes of plasmid DNA (5 ml x 18 tubes)

VerticalP100VT

P100VT* 9023063M 100,000 700,000 5 x 8 6 Rapid separation of plasmid DNA

P65VT2* 9023023M 65,000 416,000 5 x 16 10 Separation of plasmid DNA etc. by the sedimentation equilibrium centrifugation P65VT3* 9023033M 65,000 402,000 12 x 10 13

P50VT2* 9023083M 50,000 243,000 40 x 8 36 Density gradient centrifugation of large volume samples

Swinging bucketP28S

P65ST 9022391M 65,000 419,000 5 x 3 48 Density gradient centrifugation of protein, DNA and RNA, etc.

P56ST 9022551M 56,000 409,000 4 x 6 54 Density gradient centrifugation of micro volume samplesSucrose density gradient centrifugation of protein, DNAP55ST2 9022771M 55,000 366,000 5 x 6 50

P40ST 9022371M 40,000 284,000 13 x 6 139 Density gradient centrifugation of intermediate volume samplesSucrose density gradient centrifugation of virus particlesP28S 9022881M 28,000 141,000 40 x 6 252

ZonalP32ZT

P32ZT 9023160M 32,000 102,000 1,690 ml 363 Large-scale purification of protein and virus particles by density gradient centrifugation

Continuous flowP32CT

P32CT 9022660M 32,000 102,000 430 ml 42 Continuous concentration of large virus particles

1. Automatic life management rotors, CP-α, CP-MX and CP-WX series-compatible only (automatic RLM system).

2. * They can be purchased from "Rotor Guide" on the website. (http://centrifuges.hitachi-koki.com/rotor/)

3. All the rotors above are also compatible with the CP-α, CP-MX and CP-WX, series.4. Rotors with model names that include the letter T are made of titanium alloy.5. When model names do not include the letter T, rotors are made of aluminum alloy.6. Buckets for swinging bucket rotors are made of titanium alloy.

7. When using the zonal rotor P32ZT, the RPZ-S zonal rotor seal attachment ass'y., Part No. 90130600, is required (order separately). Please refer to p. 5.

8. When using a seal tube, please place a separate order for tube sealer and tube rack.9. ● indicates a made-to-order rotor.10. Capacity in the above table shows nominal capacity of tubes. Actual capacity in actual use

may be smaller than the nominal capacity depending on rotor structure, tube shape, etc.11. As for P32CT, refer to p. 5.12. The P28S rotor can also be used for 16 ml x 6 pcs., with optional 16 Ti bucket ass'y.

(Part No. 347607A, order separately).

Notes:

Unique rotors

P50A3 P32ATThe P50A3 fixed angle rotor can separate samples contains in twenty-four 1.5 ml microtubes at a time. It is suitable for speedy pelleting of minimal volume samples in nano-order. Fluid annulus prevents the sample leakage even if the sample overflows from a tube in the rotor during the rotor is spinning. Applicable microtube is "himac 1.5 ml microtube" (S308892A, 300pcs/box) only.

The P32AT fixed angle rotor can separate samples contained in thirty-two 12 ml tubes at the same RCF at a time. It is suitable for separation of many samples in nano-order at 100,000 x g or higher RCF. Fluid annulus prevents the sample leakage even if the sample overflows from a tube in the rotor during the rotor is spinning. Tube cavity angle 35° in two layers provide the same RCF to all tubes (32 tubes) for efficient pelleting.

6Ultracentrifuges

Specifications

Note: Windows®, Windows® XP, Windows Vista® and Windows® 7 are registered trademark of Microsoft Corporation in the United States and other countries.

• All specifications are subject to change without advanced notice.• Actual color may vary from the color of the photos on this catalogue, due to printing conditions.• Due to safety reasons, installation environments, operating environments and conditions may be restricted.• Unless specially mentioned, products and/or operation panel of the photos are standard specification.• For further information, please contact your nearest Hitachi Koki’s representative.

1-8-12 Shiba-Kouen, MinatoTokyo 105-0011 JAPANTEL : +81-3-5733-0206FAX : +81-3-5733-0207

URL : http://centrifuges.hitachi-koki.com

Model CP100WX CP90WX CP80WX

Max. speed (rpm) 100,000 90,000 80,000

Max. RCF(xg) 803,000 (P100AT2 rotor) 700,000 (P90AT rotor) 615,000 (P80AT rotor)

Speed control accuracy (rpm) ±10 (1,000 to maximum speed)

Set speed (rpm) 1,000 to maximum speed in increments of 100

Displaying and setting RCF Applicable (RCF max. or RCF average)

ω2T run Applicable

Drive unit warranty 10 years

Rotor temperature control accuracy (°C) ±0.5 (set temperature is from 0 to +40)

Cooling method Thermo-module cooling (CFC/HCFC/HFC-free)

Operation panelColor liquid crystal display (256 color) and keyboard

Touch-sensitive panel (run screen only)

Rotor life managementRLM rotor: automatic management

Rotor with optical adaptor: management by registration system

Data communication /Log Management system

LAN board (option), himac Log Manager Ver. 3.1* (optional software)Compatible OS: Windows® XP, Windows Vista®, Windows® 7

Vacuum system Oil-rotary vacuum pump with moisture removal function and oil diffusion pump

HEPA filter Option

Operational noise (dB(A)) 53

Heat radiation into the room 1 kW or less

Dimensions (w x h x d)31 x 27 x 39 in (790 x 690 x 1000 mm);

depth with safety cover: 35 in (890 mm); height to table: 33½ in (850 mm)

Floor area 8¾ ft2 (35½ x 35½ in) [0.81 m2 (900 x 900 mm)]

Weight 882 lbs (400 kg)

Power requirementsSingle phase, 50/60 Hz,

AC 200/208/220 V ±10%, 20 A max. (normally 8A) AC 230/240 V ±10%, 16 A max. (normally 7A)

StandardCE marking qualified

EN 61010-2-020, EN61326-1, EN61000-3-2*, EN 61000-3-3*

*These standards are applicable to the product when AC230/240V is selected.

HLI-7B 2013.07

Learn more at nuaire.com/hitachi

Sales and Support in the US and CA by:

NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447

tf: 800.328.3352 | ph: 763.553.1270 | fx: 763.553.0459