ultimate in vivo imaging and automatic dxa analysis for
TRANSCRIPT
Ultimate In Vivo Imaging and Automatic DXA Analysis for Longitudinal Studies
DXA(Dual energy X-ray Absorptiometry) as Solution
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VET DXA New Gold Standard
1. Why Osteosys?
2. What is DXA?
3. iNSiGHT: Special Feature
4. iNSiGHT: Imaging Analysis
Why Osteosys?
COMPANY HISTORY: ENTHUSIASTIC RESEARCHER
An enthusiastic young professor wanted to make a “better product for better life”for the people who were suffered by osteoporosis.To realize his dream, our strong R&D center launches a new product every 2 years.
1998 2000 2001 2004 2005 2008 2011 2013 2015 2018
Studied BMD Technology at small Lab. In KonKukUniversity
Established Osteosys with1st Gen. QUS
Launched 1st Gen. pDXA(Peripheral Cone Beam DXA)
Launched2nd Gen. QUS (Waterless probe)
Launched1st Central DXA(First pencil
DXA)
Launched 2nd Gen. Pencil beam
beam table type DXA(compact design& fold wing bedDown)
Launched 2nd Gen. pDXA
Launched 1st Gen. Whole body DXA(with Body composition & Assessment)
Launched1st GenCentral smart Fan beam DXA
Launched 3rd GenQUS(Mobile accessibility)
2019
Animal DXA
FAST GROWING: BMD Market Share (2018)
China : Approx. 60% (QUS)
Color distribution in Korea
> > > > >> >>
Korea market : 80%
With the good quality and cost-effective BMD devices,Osteosys is dominant in the domestic market, and main player in Chinese market.
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Full Line Up BMD of Osteosys
QUS
PDXA
DXAPencil beam
DXAFan beam
SHARE IT WORLDWIDE
We would like to share our BMD with the people around the world.
more than 100 countries 130 worldwide sales networks
7,000 DXA 11,000 QUS installation reference
Scanning Methods Traditional X-rays cannot measure bone density. A number of different types of BMD tests are available, but the gold standard is DXA.
1) DXA (Dual-energy X-ray Absorptiometry) measures the spine, hip or total body
2) SXA (single-energy X-ray absorptiometry) measures the heel or wrist
3) DPA (dual photon absorptiometry) measures the spine, hip or total body
4) SPA (single photon absorptiometry) measures the wrist
5) QCT (Quantitative Computed Tomography) measures the spine or hip
6) PQCT (peripheral QCT) measures the forearm
7) QUS (Quantitative Ultrasound) uses sound waves to measure the heel or finger
WHO Gold standard
WHO defines DXA
as gold standard BMD technology with the most accuracy
What is DXA ?
DXA(Dual energy X-ray Absorptiometry) is a measurement technology based on the variable absorption of X-ray by the different body components and uses high and low energy X-ray photons.
1. X-ray beam penetrates both bone and soft tissue.
2. Low and High Energy attenuation coefficients and mass absorption coefficients for Bone & Soft
tissues are different.
3. Acquire photon counts before and after X-ray in each tissue. (Io, I).
4. Calculate the system of simultaneous equations by putting the 𝐼ℎ𝑜 and 𝐼𝑙𝑜 values into a common
denominator using the equations for the high energy 𝐼ℎ value and the low energy 𝐼𝑙 value.
5. It is possible to get the areal densities (g/cm^2) of the bone and soft-tissue, and by this value
we can calculate the mass and ratio.
Cone Beam DXA
pDXA(Peripheral Dual-energy
X-ray Absorptiometry)
Fast operation by
Cone Beam X-ray
Mono Tank
(Tube & Generator)
CCD camera detector
QUS
QUS(Quantitative Ultrasound
System)
Safe operation by
Ultrasound wave
SOS: Sound of Speed
BUA: Broadband
Ultrasound
Attenuation
Fan beam DXA
Fan beam DXA(Fan beam Dual-X ray
Absorptiometry)
Most efficient fan
beam technology to
cover large scan area
and high speed scan
(fan beam technology)
X-ray Point source
Detector
0.5 MHz
Ultrasonic transducer
Pencil beam source
Pencil beam DXA
Central DXA(Central Dual-energy
X-ray Absorptiometry)
Best precision bone
Densitometry as Gold
Standard which is
recommended by
WHO
Detector
Fan beam source
Detector array
BMD technology of Osteosys
iNSiGHT : Special Feature
DXA TECHONOLGY
1 NON-INVASIVE ANALYSIS
2 LONGITUDIANL IN-VIVO F/U
3BODY COMPOSITIN ANALYSIS FOR WHOE BODY AND EACH REGION OF INTEREST
4ACCURATE RESULTS FOR LEAN/FAT/BONE (<CV 1%, 𝑅2 > 0.99)
5 FAST SCAN( <25 sec)
6 QUICK&EASY PRE-TREATMENT
7NO RADIATION FOR RESEARCHER& MINUMUM DOSE FOR ANIMAL
DXA System Bone for Laboratory Animals- Mineral Density and Body Composition Analysis
FLAT PANEL DETECTOR
HIGH RESOLUTION (Pixel size of 100µ𝒎)
DR IMAGES FOR BONE/CARTILAGE, FAT, LEAN DISTRIBUTION ANALYSIS
WIDE SCAN AREA(16.5cmX25.5cm)
DXA FEATURES DESCRIPTION
NON-INVASIVE ANALYSISMaximize cost and labor savings through non-invasive analysis, rather than traditional chemical composition analysis, which was expensive and energy intensive.
LONGITUDIANL IN-VIVO F/U
Follow-up of body composition and bone density changes in the In-Vivo situation is possible without sacrifice of experimental animals.
ACCURATE RESULTS FOR LEAN/FAT/BONE
(<CV 1%, 𝑅2 > 0.99)
Measurement error within 1% (Bone; Precision Error & Accuracy Error in Static Condition). Precision: Compared to Chemical component analysis, 𝑅^2=0.99 or higher
FAST SCAN(<25 sec)
It is a Cone Beam type technology that captures a subject at a time; allows the shortest time among Lab DXA equipment unlike Fan Beam technology that scans a subject by “scratching”.
QUICK&EASY PRE-TREATMENT
No need to dissect experimental animals, only simple injection / breathing anesthesia; Maximize the time and labor savings of pretreatment.
NO RADIATION FOR RESEARCHER & MINUMUM
DOSE FOR ANIMAL
Full blockade of the researcher's radiation exposure by a cabinet-type full shield. Minimization of animal exposure dose compared to micro CT.
FLAT PANEL DETECTOR FEATURES
DESCRIPTION
HIGH RESOLUTION (Pixel size of 100µ𝒎)
100µ𝒎 a-Si TFT flat panel detector provides 16lp / mm high resolution image, better than 3.5lp / mm standard DR (4x magnification mode).
DR IMAGES FOR BONE/CARTILAGE, FAT, LEAN DISTRIBUTION
ANALYSIS
Through high resolution image, it is possible to track changes in bone, cartilage, fat distribution, and muscle on a daily, weekly and monthly basis after treatment of medication, exercise therapy, and functional food.
WIDE SCAN AREA(16.5cmX25.5cm) Wide scan area; Small animals up to 500g can be measured.
iNSiGHT : Software
Emergency switch
Shooting plate
Digital DetectorPlate
Radiation shielding window
Cone beam HFG
Power Switch
Scan Time 25sec.
Measurement Window for each ROI
Parameter UnitDescription
(FOR TOTAL BODY & EACH ROI)
BMC gBone Mineral Contents(Bone Mass);
= Density X Area
FAT g FAT Mass
FAT Ratio %Tota l Body Fat Rat io
=FAT/Tota l Mass
LEAN g Soft Tissue – Fat Mass
Total Mass gTota l Mass
=FAT+LEAN+BONE
BMD g/㎠ Bone Mineral Density
Bone Area ㎠ Bone Area of the Image
Tissue Area ㎠ Tissue area of the Image
Color Mapping for Fat/Lean Visualization
X-ray Image by iNSiGHT Bone-Enhanced Image
DR Image
Skull
Femur
History Analysis for Each ROI
Length ROI Setting- Measure only length
Analysis ROI setting function- Compatible with current SW
Color Visualizing Function (1)- Indicate specific parameter separately
Color Visualizing Function (2)- Overlapping with X-ray image (transparency adjustable)
Color Visualizing Function (3)- Adjustable color of each parameter
VET DXA iNSiGHT
iNSiGHT : Clinical Evidence
Objectives
We examined the precision, accuracy, and capability of detecting changes of Dual-Energy X-ray Absorptiometry(DXA) for the measurements of total-body weight(TBW), total-body fat weight(TBFW), and total-body lean weight(TBLW) in in an 8-week follow-up study of rats.
Materials and Methods20 male rats(4-week) were divided into 2 diet groups. For 8 weeks, we measured body composition(TBW, TBFW, TBLW) by DXA and TBW by an
electronic scale once a week. In week 8, we measured body composition 5 times by DXA and TBFW by dissecting experiment(EXP) of euthanized rats(12-week). Total-body fat
ratio(TBFR) was defined as TBFW / (TBFW+TBLW). The precision of DXA was evaluated by measuring the coefficient of variation(CV) and accuracy was evaluated by comparing DXA-derived data with EXP data.
The capability of detecting changes of DXA in follow-up study was verified by analyzing the trend of DXA-derived values over the 8 weeks.
ResultsFor TBW, TBFW, TBLW of DXA, CVs were 0.02±0.01, 0.10±0.05, 0.03±0.02 and errors were -6.996 ±3.429 (r=0.999), +14.729 ±3.663 (r=0.982), -21.725 ±4.223 (r=0.991). Prediction models were [EXP TBW = -31.767 +1.085(DXA
TBW), R2=0.998, root mean square error (RMSE)=1.842] and [EXP TBFR = -0.056 0.056 +1.177(DXA TBFR), R2=0.948, RMSE=0.007]. Over 8 weeks, DXA TBW and and DXA TBLW steadily increased, DXA TBFW steadily increased followed by saturation ordeclination, difference of DXA TBFW between 2 diet groups steadily increased.
Conclusions
Our study verified that DXA(iNSiGHT VET DXA, OsteoSys, Korea) is accurate and and precise enough to measure body composition of rats. Additionally, we confirmed the possibility that DXA could be used for the long-term follow-up studies.
VET DXA iNSiGHT
iNSiGHT : Comparative Devices
Micro CTDXAs for Animal NMR
Function iNSiGHT NMR Micro CT
3D Image N/A N/A YES
Slice Image N/A N/A YES
2D DR Image YES N/A YES
BMD(g/㎠) YES N/A
PARTLY YES(BY GRAY SCALE MAPPING, NOT BY DXA’S MATERIAL ANALAYSIS. LESS ACCUARATE THAN DXA)
BMC(g) YES N/A
YES(BY GRAY SCALE MAPPING, NOT BY DXA’S MATERIAL ANALAYSIS. LESS ACCUARATE THAN DXA)
FAT(g) YES YESOPTIONAL
(DEPENDS ON DEVICE)
LEAN(g) YES YESOPTIONAL
(DEPENDS ON DEVICE)
FAT(%) YES YES N/A
Bone Area(㎠) YES N/A N/A
Free Body Fluid(Water) N/A YES N/A
Heavy Animal(500g) Measurement
YES N/A N/A
Price LOW MIDDLE HIGH
Cost of Ownership LOW MIDDLE HIGH
Item Comment InAlyzer iNSiGHT VET DXA
Specifications
X-Ray System Equivalent DXA(Dual Energy X-ray Absorptiometry) DXA(Dual Energy X-ray Absorptiometry)
Scan Method INSIGHT Better (Faster Scanning) Fan Beam Cone Beam
Scan Site Equivalent Small Animal Total Body Small Animal Total Body
Scan TimeINSIGHT Better (X-ray Exposure
and Total Scan time)
(fast mode)x-ray time = 25s
scan time = 60s under
(common mode)x-ray time = 10s
scan time = 25s under
Measurment Parameter EquivalentBody Composition (Fat/ Lean/ BMC/ BM
D / FAT%)BMD, BMC, Bone/Tissue Area, Fat%, Fat(g), Lean(g), Total Weight @ each ROI
Precision Equivalent 1% (CV)<1% (CV) @phantom
<1% (CV) @total body weight of mouse, rat (short term,fixed position, n = 5)
AccuracyUndetermined (different unit
values provided)1% (CV)
>0.99 (R2) @phantom>0.99 (R2) @total body weight of mous
e, rat (n=20)
Positioning Equivalent Center preffered, in scan area lineCenter preffered, in scan area line of eac
h magnification level
Image areaInAlyzer Better
* Note: 2 different model existed210 mm X 315 mm, 140 mm X 210 mm 165mm x 255mm @1.2X
Pixel size INSIGHT Better 108 x 108 um 100µm @1.2X, 31µm @4X
Operating System Undetermined Windows 7/ 8 (32bit, 64bit) Windows 10 64bit (recommended)
Monitor Resoltution INSIGHT Better 1280 x 768 (1920 x 1080) 1920 x 1080
DimensionInAlyzer Better (Smaller the better)
* Note: Longer SID of iNSiGHTallows less distortion
740(W) x 430(D) x 717(H) 660(W) x 605(D) x 1130(H)
Weight InAlyzer Better (Lighter the better) 95kg 160Kg
Power/ Environment Equivalent 90 ~ 264VAC 110/240VAC, 50/60Hz, 200VA
Operating Temp Equivalent 10 ~ 35 ℃20 ~ 25 ℃ (recommended), 10 ~ 35 ℃ (
maximum)
Features
Size of AnimalInAlyzer Better
* Note: Officially, maximum size is rat (Mainly Mouse)
Small animal such as mouse(10~50g) or Gerbil, Middel size such as Rabbit,
Guinea Pig, Dog, Cat
Small animals less than 500g (Upcoming upgrade)
The number of Body Composition ROI
INSIGHT Better Maximum 5 Manual ROIs Unlimited Manual ROI
Efficiency of Analyze INSIGHT Better
X-ray irradiation time less than 30 seconds in one measurement,
measurement result can be checked within 60 seconds (Optimum mode
180sec, Accuracy mode 300sec)
X-ray irradiation time less than 10 seconds in one measurement, measurement result can be checked within 30 seconds (Excluding ROI setting time)
Precision Equivalent Less than 1% CVLess than 1% CV
(for Phantom and fixed animal)
Radiation Shielding Undetermined (No detailed info) Raditation Shielding (No detailed info)below 10mRem per examination (within
5m)Real-time Measurement
StatusUndetermined (Pros & Cons for
each product)Built-in Camera lead glass window (upcoming upgrade)
Back-up EquivalentData and Image can be saved as Excel
and Tiff formatExtract result data (csv format),
view image (bmp format)
Resoultion of Image
INSIGHT Better* Note: Magnification available,
HQ DR applied
54um linear detector; 5.0lp/mm (Better than 3.5lp/mm DR)
*Note: Error in the specification - sensor resolution [lp/mm] = 1000 / (2 * pixel
size [um])
Provides a high-resolution image of 5.0 lp/mm (@ x1.2 magnification), 16.0 lp/m
m (@ 4X magnification)
Item Comment InAlyzer iNSiGHT VET DXA
VET DXA New Gold Standard
FAST Scan 25sec.
DXA Original
Technology
DR Image
Customized Feedback
Accuracy : R2 >0.9Precision : CV<1%
Simple Operation