uk cord blood & tissue collection protocol

UK CORD BLOOD ANDTISSUE PROCUREMENT PROTOCOL

These instructions must not be followed unless the procurer is trainedand acting under an HTA licence or in pursuance of a Third Party

Agreement with Cells4Life

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CELLS4LIFE COLLECTION PROTOCOL

Please read and follow these instructions carefully

Cells4Life couriers operate 24 hours a day 365 days a year.

To activate the courier service please call the dedicatedcollection line with your customer number. This number can befound on your kit box and the accompanying dispatch letter.

• In the event of a complicated delivery, failure of collectionor for immediate technical advice during delivery, please callto speak to a medical advisor, available 24 hours a day.

• The collection kit contains materials for the maternal,umbilical cord blood and/or tissue collection. Please ensure,where possible, ALL samples are taken and returned via ourmedical couriers.

• Please note it is the responsibility of the partner or husbandand not the midwife or doctor to arrange for the courier tocollect the sample.

If you have any queries please do not hesitate to contact us.

Wayne M ChannonChairman

Combined Blood & Tissue A5 Booklet:Layout 1 30/3/11 13:04 Page 2

Remove the sealed Cord BloodCollection Kit from thetransportation box. Check theintegrity of the sealed packaging.

Tear open the sealed Cord BloodCollection Kit packagingas shown.

Cord Blood Collection Kit contents:1 & 7. Cord blood collection bag2. Gauze swabs3. Chlorhexidine swabs4. Cord blood packaging5. Maternal sample kit6. Auxilliary blood collection items

Following delivery of theplacenta quickly check for grossabnormalities. If in utero detailwhy on Collection Summary Form.

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CORD BLOOD PROCUREMENT


Using the sterile gauze, removeexcess fluid and debris.

Using the chlorhexidine swabs,liberally disinfect the proposedneedle insertion point.

Insert the needle from onecollection bag into the umbilicalvein close to the cut end. If bloodflow becomes poor or stops, usethe second collection bag insertingthe needle into the umbilical veinclose to its origin at the placentato maximise blood collection.

Allow blood to drain into thecollection bag by gravity flow.Agitate bag occasionally to mixblood and anticoagulant. Milkingthe cord and manipulating theplacenta will help flow.

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Lock off the blood line with theattached clamp and knot for extrasecurity. Cut off needles anddispose in sharps bin.IF TWINS ARE DELIVERED ENSURE THEBAGS ARE CLEARLY IDENTIFIABLE

Take maternal blood sample usingthe needle and vacutainer provided.Only one needed for multiple births.If it is not possible to obtain amaternal sample then a sampleshould be taken within 7 days ofbirth and sent to us.

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Open +Cord Collection Kit ifprovided.

+Cord Collection Kit contents:1. DGP PATHOSEAL 95 (incl. absorbent pad)2. Cord collection pot3. Sterile NormasolTM saline sachets4. Forceps5. Disposable scissors6. Gauze swabs7. Piece of tissue

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CORD TISSUE PROCUREMENTIF NOT REQUIRED REFER TO POINT 20 ONWARDS.


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Select an undamaged length ofumbilical cord aproximately 10-15cmlong and cut using scissors provided.

13 Wash gloved hands thoroughly or replace gloves.

Place cord on clean tissue usingforceps and remove excess bloodand debris using sterile gauze.Suspend the cord over tissue withforceps and using sterile gauze‘squeeze’ out as much excess bloodfrom the cord as possible.

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Obtain sterile pot and remove lid.Place lid upside down on cleansurface and place cord in pot usingforceps.

17 Empty NORMASOL salinesachets into the pot,enough to cover the cord.Carefully replace lid andcheck for leaks.

18 Write client’s customernumber and date on sideof pot.

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Remove security seal and gauzefrom BioPouch. Place collected cordblood and/or tissue with thematernal sample into the BioPouch.Seal as per instructions onBioPouch.

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Complete CollectionSummary Form (overleaf).Place form and BioPouchinto kit box and seal usingsecurity label. This is alegal requirement.

MOTHER OR FATHER:SIGN THE LABEL ON THEPACKAGING

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21 Activate the courier ASAP bycalling the dedicated couriercollection line and provideyour customer number. Thecourier will provide an ETA forthe collection of the blood.

IT IS THE PARENTS’ RESPONSIBILITY TO CALL THE COURIER ANDMEET THE COURIER TO HAND OVER THE SAMPLE.

0800 4584725

PACKAGING


Collection Summary FormTo be completed by person procuring sample(s).

Please detach this section and return to Cells4Life in the collection box.

Customer/kit no.

Name of mother

Name of child (if known) M F

Name of personprocuring sample(s)

Name of person’semployer

Date of procurement

Time of procurement

Name & address whereprocurement occured

Twins Yes / No in utero collection?

if Yes, why?

Samples obtained Maternal

Cord blood

Cord tissue

Any events during procurement?

Where was sample taken

Yes / No

Yes / No

Sluice room Delivery suite Maternity ward

Other – please specify

Yes / No

Yes / No


uk cord blood & tissue collection protocol

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