Tumaini (CRT) Inc. Tumaini (CRT) Inc.

Efficacy of a New Efficacy of a New Slow Release Slow Release Mosquito Larvicide Mosquito Larvicide containing containing NovaluronNovaluron

SLOW RELEASE MOSQUITO LARVICIDE DETAILS

Larvicide contains the active ingredient novaluron and utilizes novel formulation technology which has been patented in numerous countries

Formulation controls rate of release of novaluron into water column increases solubility of novaluron in water from 3 ppb to 10-15 ppb Formulation also promotes dispersion of novaluron throughout the

water column products will be available in several different sizes and shapes to

address different market needs all products have same recipe and ai 1.2g/kg novaluron Tradename for larvicide products are:

Mosquiron 0.12P and Mosquiron 0.12CRD

•

Mosquiron 0.12P pastilles (2 sizesMosquiron 0.12P pastilles (2 sizes))

Mosquiron 0.12CRD Mosquiron 0.12CRD controlled release devicecontrolled release device

MOSQUIRON PRODUCT DETAILS

MOSQUIRON PRODUCT DETAILS

Mosquiron 2010 Field TrialsMosquiron 2010 Field TrialsPURPOSE:Assess efficacy and duration of Mosquiron products when used under simulated field conditions and in different regions of the US and Canada

Experimental Design Needed to take into consideration the mode of action of novaluronNovaluron is a chitin synthesis inhibitor and effects can be cumulative; larvae can appear healthy and mortality may not be observed until adults emerge from pupal caseAt high concentrations of novaluron (>5 ppb) effect on larvae can be rapid – 24 hoursMortality can still occur at pupal eclosion caused by incomplete separation from pupal case – adult drowning

Field Trial LocationsCanada – Portage la Prairie MB, Abbotsford BCUS – Marshall WI, Meansville GA, Panama City FL

Mosquiron 2010 Field TrialsMosquiron 2010 Field Trials

Experimental Design

Mosquiron 2010 Field TrialsMosquiron 2010 Field Trials

Mosquiron 2010 Field TrialsMosquiron 2010 Field Trials

Experimental Design Eggs were introduced to treatment tanks approx . every 2

weeks Culex eggrafts were placed directly on water surface at WI,

MB, BC and FL sites Once hatching occurred all trts. received a larval liquid

food slurry Food slurry was a standard formula consisting of liver

powder/brewer’s yeast Food administered 3-4 times/wk. until water was cloudy in

appearance

Mosquiron 2010 Field TrialsMosquiron 2010 Field Trials

Mosquiron 2010 Field TrialsMosquiron 2010 Field TrialsMarshall - WI site Meansville - GA site

Mosquiron 2010 Field TrialsMosquiron 2010 Field TrialsPanama City -FL Site

Mosquiron 2010 Field TrialsMosquiron 2010 Field TrialsPortage la Prairie - MB Site

Mosquiron 2010 Field TrialsMosquiron 2010 Field TrialsAbbotsford - BC Site

Data CollectionTreatments were inspected for the presence of live larvae and/or pupae on an as needed basis. Once 3rd and/or 4th instars were present, dips were taken to harvest live larvae. Florida A&M’s PHEREC Standard Mosquito Sampling Method was used at all trial sites. Using a standard mosquito dipper, eight dips were taken per treatment. The number of larvae and/or pupae per dip were counted and recorded. Larvae were returned to the treatment tanks. Once pupae were found, they were transferred to emergence jars for monitoring. Adults that emerged successfully from pupal cases were counted and recorded as successful hatches. Dead adult mosquitoes on the water surface recorded as unsuccessful emergence.

Mosquiron 2010 Field TrialsMosquiron 2010 Field Trials

Observations Using local sources for harvesting Culex eggrafts delayed

start of trial in Northerly sites to early July Was exacerbated by a cool wet spring in Canada and WI FL location received very high precipitation (18” rain in

July) causing significant dilution of novaluron in cement troughs

Conclusions Study results demonstrate Mosquiron formulations

provided 100% IE for duration of study at all locations 100% IE observed at FL site with 2x CRD