triple quadrupole mass spectrometer - wikipedia, the free encyclopedia
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Waters Quattro II triple quadropole mass
spectrometer (center). This photo was taken in the
old mass spec facility in Whitmore Lab of
Pennsylvania State University.
Triple quadrupole mass spectrometerFrom Wikipedia, the free encyclopedia
A triple quadrupole mass spectrometer is a tandem
mass spectrometer consisting of two quadrupole mass
spectrometers in series, with a (non mass-resolving)
radio frequency (RF)-only quadrupole between them to
act as a cell for collision-induced dissociation. The first
(Q1) and third (Q3) quadrupoles serve as mass filters.
Precursor ions selected in Q1 are dissociated in the
collision cell in the presence of an inert gas such as Ar,
He, or N2 collision-induced dissociation. Resulting
fragments are passed through to Q3 where they may be
filtered or scanned. This configuration is often
abbreviated QqQ, here Q1q2Q3.
Contents
1 Structural mass spectrometry
2 History
3 References
4 See also
Structural mass spectrometry
This alignment of two mass filters with a collision quadrupole between them allows to elucidate the structure of
the ionised sample molecules. Four main modes can be performed as follows.[1]
Product ion scan
The first quadrupole Q1 is set to select an ion of a known mass, which is fragmented in q2. The third
quadrupole Q3 is then set to scan the entire m/zrange, giving information on the sizes ofthe fragments
made. The structure of the original ion can be deduced from the ion fragmentation information. This
method is commonly performed to identify transitions used for quantification by tandem MS.
Precursor ion scan
A certain product ion is selected in Q3, and the precursor masses are scanned in Q1. This method is
selective for ions having a particular functional group (e.g., a phenyl group) released by the fragmentation
in q2.
Neutral loss scan
In this method both Q1 and Q3 are scanned together, but with a constant mass offset. This allows the
selective recognition of all ions which, by fragmentation in q2, lead to the loss of a given neutral fragment
(e.g., H2O, NH3). Similar to the precursor ion scan, this method is useful in the selective identification of
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closely-related compounds in a mixture.
Selected reaction monitoring (SRM) / Multiple reaction monitoring (MRM)
In this method both Q1 and Q3 are set to a selected mass, allowing only a distinct fragment ion from a
certain precursor ion to be detected. This method results in increased sensitivity. If Q 1 and/or Q3 is set to
more than a single mass, this configuration is called multiple reaction monitoring.[2]
History
The arrangement of three quadrupoles was first developed by Morrison of LaTrobe University, Australia for the
purpose of studying the photodissociation of gas-phase ions.[3] The first triple-quadrupole mass spectrometer
was developed at Michigan State University by Enke and Yost in the late 1970s.[4]
References
1. ^ de Hoffmann, E. (1996), "Tandem mass spectrometry: a Primer", Journal of Mass Spectrometry31 (2): 129,doi:10.1002/(SICI)1096-9888(199602)31:23.0.CO;2-T
(http://dx.doi.org/10.1002%2F%28SICI%291096-9888%28199602%2931%3A2%3C129%3A%3AAID-
JMS305%3E3.0.CO%3B2-T)
2. ^ Anderson, L.; Hunter, C. L. (2006), "Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays
for Major Plasma Proteins" (http://www.mcponline.org/content/5/4/573.full.pdf+html), Molecular & Cellular
Proteomics5 (4): 573, doi:10.1074/mcp.M500331-MCP200 (http://dx.doi.org/10.1074%2Fmcp.M500331-
MCP200)
3. ^ Morrison, J. D. (1991), "Personal reminiscences of forty years of mass spectrometry in Australia", Organic
Mass Spectrometry26 (4): 183, doi:10.1002/oms.1210260404 (http://dx.doi.org/10.1002%2Foms.1210260404)
4. ^ Yost, R. A.; Enke, C. G. (1978), "Selected ion fragmentation with a tandem quadrupole mass spectrometer"(http://masspec.scripps.edu/mshistory/timeline/time_pdf/1978_YostRA.pdf), Journal of the American Chemical
Society100 (7): 2274, doi:10.1021/ja00475a072 (http://dx.doi.org/10.1021%2Fja00475a072)
See also
Hybrid mass spectrometer
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