total hair growth solution - cap actuel · 2019. 4. 6. · 1. patented and exclusive peptide...
TRANSCRIPT
Total hair growth solution Your first experience. Your last chance.
Decapeptide-18
Octapeptide-2 Oligopeptide-54
Octapeptide-11
Decapeptide-10 Day.03
Day.06
Day.09
Day.12 Day.15
Oligopeptide-71 Decapeptide-28
Non-Scaring Alopecia Patients. Man & Woman who have a general thinning hair. Hair transplant recipients who want to increase survival rate. Alopecia patients who want to have Synergic effect combining with other application for anti-hair loss / hair re-growth.
Advantage
1. Patented and exclusive peptide complex for hair regrowth and health. 2. Sustained Release technology to maximize the efficacy of 7 hair growth peptides. 3. Applicable and effective to any type of non-scaring Alopecia. 4. Clinically proven.
Direction for use Protocol
1) Apply topical anesthetic cream on the target scalp area 2) After 30min, clean the applied cream then sterilize with alcohol swab 3) Inject DR.CYJ Hair Filler on the target area with 30G Needle ! Injection Technique: Point Injection " How to inject ! Injection point every 0.2~0.3 cm ! 1.0cm per line ! Inject 0.02~0.05 ml per point
02
DR CYJ HAIR FILLER - The 1st Hair Filler in the World. Developed by Innovative peptide complex for Hair re-growth, Scalp re-construction.
Decapeptide-18
Octapeptide-2 Oligopeptide-54
Octapeptide-11 Decapeptide-10
Day.03 Day.06 Day.09 Day.12 Day.15
Decapeptide-28 Oligopeptide-71
DAY_3 DAY_6 DAY_9 DAY_12 DAY_15
DR.CYJ Hair Filler (1 ml)
DR.CYJ Hair Filler Cocktail (1.5 ml)
Application
Standard Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session) Intensive Treatment : 1 session every 2 weeks for 8 weeks (Total: 4 Session) + 1 session every month for 3 month (up to 6 month) Post professional therapy: Apply Pelo Baum Hair revitalizing solution every morning and night on a daily basis.
DR.CYJ Hair Filler Mechanism
Hair Growth Down Regulation of DKK1 Keratinocyte Migration Angiogenesis Anti -Apoptosis
Hair Growth Fibronectin, Shh, Cdk Keratinocyte proliferation Anagen development Hair Stem Cell Proliferation (Hair stem cell differentiation) Anti-hair Loss (DDK-1 Down-regulation)
Hair Growth Hair follicle formation Anti-hair Loss (Follistatin, CTGF/CCN2 as BMP inhibitor) Hair pigmentation
03
Main Active ingredient
Decapeptide-18 (CG-WINT) Decapeptide-28
(CG-Rehairin)
Decapeptide-10 (CG-Keramin2)
Octapeptide-11 (CG-Seperin)
Oligopeptide-71 (CG-Edrin)
Oligopeptide-54 (CG-Nokkin)
Octapeptide-2 (Prohairin-�� �
In-vitro Test Data 1. Expression of Hair Growth Related Genes
Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs / Method: RT-PCR analysis Positive Control: Minoxidil 1ug/ml
HGF
600
500
400
300
200
100
VEGF
0
Control 10ng 100ng 1ug Minoxidil
GAPDH
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
500
400
300
Hair Filler Complex ( /ml)
Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell We observed HGF and VEGF expression are regulated by treatment with Hair Filler Complex. Hair Filler Complex promotes hair growth through stimulatory effect on HGF and VEGF gene expression in HHFDPC.
200
100
0
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
2. Expression of Hair Growth Related Proteins
Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs Method: Western blot analysis Positive Control: Minoxidil 1ug/ml
350
300
250
200
150
IGF-1
FGF-7
Actin
100
50
0
250
200
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
Expression of growth factors related to growth of hair follicle by Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell Hair Filler Complex promotes hair growth through stimulatory effect on IGF-1 and FGF-7 protein expression in HHFDPC.
150
100
50
0
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
3. Inhibition of DHT-induced Hair Loss Related Gene Expression
Cell: HHFDP cell p8 (Human Hair Follicle Dermal Papilla Cells) Culture time: 24 hrs Method: RT-PCR analysis Positive Control: Minoxidil 1ug/ml Stimulator: DHT 5ug/ml
Inhibition of hair loss related gene expression DHT-induced expressions of hair loss-related genes, DKK-1, IL-6 and TGF-"1 were reduced by Hair Filler Complex treatment.
Control 100ng 1ug Minoxidil
4. Inhibition of DHT-induced Cell Apoptosis Cell: HHFDP cell p6 (Human Hair Follicle Dermal Papilla Cells) Culture time: 3 days Method: SRB staining Positive Control: Minoxidil 1ug/ml Stimulator: DHT 5ug/ml
140
120
100
80
60
40
20
0
Cell growth assay after treatment of Hair Filler Complex on Human Hair Follicle Dermal Papilla Cell Hair Filler Complex inhibits DHT-induced hair cell apoptosis
Control DHT DHT+100ng/ml
Hair Filler Complex
Control 10ng 100ng 1ug Minoxidil
Hair Filler Complex ( /ml)
DHT
DHT+1ug/ml Hair Filler Complex
DHT+Minoxidil
04
VEGF 375
290 295
195
100
HGF 497
329
219 185
100
DKK-1
IL-6
TGF-"1
GAPDH
Hair Filler Complex ( /ml)
DHT
FGF-7 304
213 185 186
100
IGF-1 185
137 151
161
100
121
100 105 103
93
75
Rel
ativ
e ra
te ( %
of c
ontro
l)
IDV
( % o
f con
trol)
IDV
( % o
f con
trol)
IDV
( %
of c
ontr
ol)
IDV
( %
of c
ontr
ol)
CASE#06. Sex.M/Age: 28 Before After
2Months 280 31% 40
17% 210 30
140 20
70 10
0 0
188
247
104%
53
108
34%
128
171
48%
121
179
Thic
knes
s (m
m)
Thic
knes
s (m
m)
Thic
knes
s (m
m)
Human Clinical Study Before vs After
* 1session every 2 weeks for 8weeks (4 sessions)
CASE#01. Sex.M/Age: 46 Before After 2Months
200
150
100
50
0
Before After
40
30
20
10
0
CASE#02. Sex.M/Age: 45 Before After 2Months
200 40
150 30
100 20
50 10
0 0
CASE#03. Sex.M/Age: 47 Before After 2Months
200 40
150 30
100 20
50 10
0 0
CASE#04. Sex.F/Age: 38 Before After 2Months
200 40
150 30
100 20
50 10
0 0
37.7
17%
32.2
Hair Thickness [mm]
35%
117
158 24%
23.3
29
45%
14.2
9.8
35%
1
21.5
5.9
42%
21.8
30.9
Total Hair Density [1/cm2]
Num
ber
Num
ber
Num
ber
Num
ber
Num
ber
Thic
knes
s (m
m)
Thic
knes
s (m
m)
[Published in June. 2017] ⓒ 2017 Caregen Co.,Ltd