top-selling elisa kits 2016 - elabscience

Elabscience®

Focus on lab research Service for life science

ELISA KIT

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Elabscience specializes in immunodiagnostic technology for the Life Science community. We have complete R&D and manufacture platform and have good quality control for every kit by every lot. We endeavor to reduce lot to lot variation to keep your experiment results more consistent and precise. Through unremitting efforts and development, Elabscience’s products have gained more market share, customers are now all over the world in more than 100 countries.Our major products are Proteins, Antibodies, ELISA Kits, CLIA Kits, Labeling Kits and related reagents.Elabscience also provides custom services for our customers including Protein Expression and Purification Service, Antibody Preparation Service, ELISA Customized Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.

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Focus on Lab Research, Service for Life Science.

Application areas

Why choosing Elabsience?

Advantages:

High Stability

Full Range of Products

Efficient Delivery

Reliable Supply Chain

Perfect Technical Support and After-sale Service

Autoimmunity

Enzyme & Kinase

EndocrinologyImmunomodulator

Apoptosis

Cardiovascular disease

Cancer

Hormone

GlobulinsHematology

Hypersensitivity

Respiratory system

Cytokines & Growth Factors

Innate immunity

Development

Infectious Diseases

Urinary system

Stem cell

Digestive system

Metabolism

Neuroscience

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Cat.No Product Name Detection Range Sensitivity

E-EL-H0543 Human Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H0010 Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H0483 Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit 12.5--800pg/mL 7.5pg/mL

E-EL-H0622 Human CACYBP (Calcyclin Binding Protein) ELISA Kit 78.125--5000pg/mL 46.875pg/mL

E-EL-H0130 Human COL1 (Collagen Type I) ELISA Kit 0.625--40ng/mL 0.375ng/mL

E-EL-H0851 Human CPP (Copeptin) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H0043 Human CRP (C-Reactive Protein) ELISA Kit 0.781--50ng/mL 0.469ng/mL

E-EL-H0835 Human CTXI (Cross Linked C-telopeptide of Type I Collagen)ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-H0864 Human D2D (D-Dimer)ELISA Kit 78.125--5000ng/mL 64.875ng/mL

E-EL-H0059 Human EGF (Epidermal Growth Factor) ELISA Kit 3.906--250pg/mL 2.344pg/mL

E-EL-H0064 Human ET-1 (Endothelin 1) ELISA Kit 1.25--80pg/mL 0.75pg/mL

E-EL-H2237 Human GC (Glucagon) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H1908 Human GDF11 (Growth Differentiation Factor 11) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H0177 Human GH (Growth Hormone) ELISA Kit 78.125--5000pg/mL 64.875pg/mL

E-EL-H0148 Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-H0081 Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit 7.813--500pg/mL 4.688pg/mL

E-EL-H5497 Human Hepc25 (Hepcidin 25) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-H1554 Human HMGB-1(High Mobility Group Protein B1)ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H2532 Human IFN-α (Interferon Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H0085 Human IFN-β(Interferon Beta)ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H0108 Human IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H2161 Human IgE (Immunoglobulin E) ELISA Kit 3.125--200ng/mL 1.875ng/mL

E-EL-H0086 Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-H0103 Human IL-10 (Interleukin 10) ELISA Kit 7.813--500pg/mL 4.688pg/mL



E-EL-H0149 Human IL-1β (Interleukin 1 Beta) ELISA Kit 7.813--500pg/mL 4.688pg/mL

E-EL-H0099 Human IL-2 (Interleukin 2) ELISA Kit 125--8000pg/mL 75pg/mL





Top-selling Kits

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E-EL-H0113 Human LEP (Leptin) ELISA Kit 78.125--5000pg/mL 46.875pg/mL

E-EL-H0020 Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H0134 Human MMP-13 (Matrix Metalloproteinase 13) ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-H1446 Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-H1451 Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H2016 Human MT (Melatonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H1386 Human NFκB (Nuclear Factor Kappa B) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-H0096 Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-H1893 Human NPY (Neuropeptide Y) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H1047 Human NSE (Neuron-Specific Enolase) ELISA Kit 2.344--150ng/mL 1.406ng/mL

E-EL-H2234 Human PI (Proinsulin) ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-H0183 Human PIIINP (N-Terminal Procollagen III Propeptide)ELISA Kit 23.438--1500pg/mL 14.063pg/mL

E-EL-H1476 Human PTH (Parathyroid Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H1297 Human S100B (S100 Calcium Binding Protein B) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H1487 Human TG (Thyroglobulin) ELISA Kit 125--8000ng/mL 75ng/mL

E-EL-H0110 Human TGF-β1 (Transforming Growth Factor Beta 1)ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H0109 Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 7.813--500pg/mL 4.688pg/mL

E-EL-H0111 Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-R0048 Rat ACTH (Adrencocorticotropic Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0329 Rat ADP/Acrp30 (Adiponectin) ELISA Kit 46.875--3000pg/mL 28.125pg/mL

E-EL-R1430 Rat ANG II (Angiotensin II) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0126 Rat BNP (Brain Natriuretic Peptide) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-R0135 Rat CGRP (Calcitonin Gene Related Peptide) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0269 Rat CORT (Corticosterone) ELISA Kit 78.125--5000pg/mL 46.875pg/mL

E-EL-R0007 Rat EPO (Erythropoietin) ELISA Kit 62.5--4000pg/mL 37.5pg/mL

E-EL-R0167 Rat ET-1 (Endothelin 1) ELISA Kit 7.813--500pg/mL 4.688pg/mL

E-EL-R0391 Rat FSH (Follicle Stimulating Hormone) ELISA Kit 3.125--200ng/mL 1.875ng/mL

E-EL-R0029 Rat GH (Growth Hormone) ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-R0506 Rat hs-CRP (High Sensitivity C-Reactive Protein) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-R0009 Rat IFN-γ (Interferon Gamma) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-R0016 Rat IL-10 (Interleukin 10) ELISA Kit 31.25--2000pg/mL 18.75pg/mL


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E-EL-R0011 Rat IL-1α (Interleukin 1 Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0012 Rat IL-1β (Interleukin 1 Beta) ELISA Kit 31.25--2000pg/mL 18.75pg/mL




E-EL-R2466 Rat INS (Insulin) ELISA Kit 3.125--200ng/mL 1.875ng/mL

E-EL-R0582 Rat LEP (Leptin) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-R0026 Rat LH (Luteinizing Hormone) ELISA Kit 1.563--100mIU/mL 0.938mIU/mL

E-EL-R0025 Rat MAU (Microalbuminuria) ELISA Kit 1.563--100μg/mL 0.938μg/mL

E-EL-R0031 Rat MT (Melatonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0520 Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-R0243 Rat OC/BGP (Osteocalcin) ELISA Kit 0.781--50ng/mL 0.469ng/mL

E-EL-R2400 Rat PCT (Procalcitonin) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-R0046 Rat sICAM-1/CD54 (Intercellular Adhesion Molecule-1)ELISA Kit 0.391--25ng/mL 0.234ng/mL

E-EL-R0084 Rat TGF-β1(Transforming Growth Factor β1) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-R0540 Rat TIMP-1(Tissue Inhibitors of Metalloproteinase 1)ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-R0019 Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 78.125--5000pg/mL 46.875pg/mL

E-EL-R0054 Rat Tn-T (Troponin T) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M1272 Mouse ALT (Alanine Transaminase) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-M0160 Mouse AST (Aspartate Aminotransferase) ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-M0068 Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0339 Mouse C5a (Complement Component 5a) ELISA Kit 62.5--4000pg/mL 37.5pg/mL

E-EL-M0053 Mouse CRP (C-Reactive Protein) ELISA Kit 0.391--25ng/mL 0.234ng/mL

E-EL-M0027 Mouse EPO (Erythropoietin) ELISA Kit 78.125--5000pg/mL 64.875pg/mL

E-EL-M0090 Mouse GLP-1 (Glucagon Like Peptide 1)ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0676 Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0713 Mouse IFN-α (Interferon Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0033 Mouse IFN-β(Interferon Beta)ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0048 Mouse IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0690 Mouse IgA (Immunoglobulin A) ELISA Kit 6.25--400ng/mL 3.75ng/mL

E-EL-M0034 Mouse IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit 78.125--5000pg/mL 64.875pg/mL


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E-EL-M0692 Mouse IgG (Immunoglobulin G) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-M0046 Mouse IL-10 (Interleukin 10) ELISA Kit 15.625--1000pg/mL 9.375pg/mL



E-EL-M0037 Mouse IL-1β (Interleukin 1 Beta) ELISA Kit 7.813--500pg/mL 4.688pg/mL




E-EL-M2614 Mouse INS(Insulin) ELISA Kit 3.125--200ng/mL 1.875ng/mL

E-EL-M0039 Mouse LEP (Leptin) ELISA Kit 125--8000pg/mL 75pg/mL

E-EL-M0006 Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0627 Mouse MMP-9 (Matrix Metalloproteinase-9) ELISA Kit 78.125--5000pg/mL 64.875pg/mL

E-EL-M0864 Mouse OC/BGP (Osteocalcin) ELISA Kit 0.391--25ng/mL 0.234ng/mL

E-EL-M0051 Mouse TGF-β1 (Transforming Growth Factor β1) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-M0641 Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)ELISA Kit 0.156--10ng/mL 0.094ng/mL

E-EL-M0049 Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-Ch0026 Chicken IFN-γ (Interferon Gamma) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-Ch1621 Chicken IL-10 (Interleukin 10) ELISA Kit 15.625--1000pg/mL 9.375pg/mL



E-EL-Ch0228 Chicken IL-6(Interleukin 6) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-P0003 Porcine IFN-γ (Interferon Gamma) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-P0004 Porcine IgG (Immunoglobulin G) ELISA Kit 3.125--200ng/mL 1.875ng/mL

E-EL-P0020 Porcine IL-6 (Interleukin 6) ELISA Kit 125--8000pg/mL 75pg/mL

E-EL-P0010 Porcine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-GP0002 Guinea pig IgG (Immunoglobulin G) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-MK2266 Monkey CIC(Circulating Immune Complex)ELISA Kit 0.313--20ug/mL 0.188ug/mL

E-EL-RB0011 Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-0028 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-0056 cAMP (Cyclic Adenosine Monophosphate) ELISA Kit 1.563--100ng/mL 0.938ng/mL

E-EL-0083 cGMP(Cyclic GMP)ELISA Kit 0.781--50pmol/mL 0.469pmol/mL

E-EL-0030 Cortisol ELISA Kit 12.5--800ng/mL 7.5ng/mL


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E-EL-H0137 Human ACTH (Adrenocorticotropic Hormone) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-H0004 Human ADP/Acrp30 (Adiponectin) ELISA Kit 0.781--50ng/mL 0.469ng/mL

E-EL-H0317 Human AMH (Anti-Mullerian Hormone) ELISA Kit 93.75--6000pg/mL 56.25pg/mL

E-EL-H0008 Human ANG2 (Angiopoietin 2) ELISA Kit 46.875--3000pg/mL 28.125pg/mL

E-EL-H0326 Human Ang-II (Angiotensin II) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-H0542 Human Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit 15.625--1000pg/mL 9.375pg/mL

E-EL-0065 E2 (Estradiol) ELISA Kit 21.875--1400pg/mL 13.125pg/mL

E-EL-0045 EPI (Epinephrine/Adrenaline) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

E-EL-0067 SP (Substance P) ELISA Kit 78.125--5000pg/mL 46.875pg/mL

E-EL-0072 T (Testosterone) ELISA Kit 0.313--20ng/mL 0.188ng/mL

E-EL-0046 DA (Dopamine) ELISA Kit 31.25--2000pg/mL 18.75pg/mL

1.Some tips for ELISA failure

2.Poor Precision in ELISA experiment

ELISA failure can be attributed to many factors. Many unexpected results can be avoided if the manual is

read and fully understood before starting the experiment. On identifying assay failure, you can follow tips below.

1. Check the expiration dates of the kit, ensure that all the reagents have been stored as indicated in the

manual.

2. Check for signs of instability or deterioration in reagent solutions, such as precipitation or discoloration and

so on.

3. Substrate reagent should be colorless.

4. Use clean plastic disposable pipettes, tips, and containers for reagent preparation and storage. Avoid

cross-contamination of reagents by changing pipette tips between addition of each standard, sample and reagent.

5. Ensure that specified incubation times and temperatures have been adhered to.

6. Ensure that no substitution of kit reagents has occurred.

7. It is recommended that samples and standards be run in duplicate to improve accuracy.

Possible causes Solutions

Incomplete washing of wells

Inadequate aspiration of wells

Ensure that wash apparatus is working correctlyEnsure adequate time of soaking

Wells should appear dry after aspiration


Frequently Asked Questions

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3.Poor Standard Curve in ELISA experiment

4.High Background in ELISA experiment

5.Too much signal in ELISA experiment

6.Standard curve achieved but poor discrimination

Possible causes

Possible causes

Possible causes

Possible causes

Solutions

Solutions

Solutions

Solutions

Improper dilution of standard

Insufficient washing

Incomplete washing of wells

Not enough HRP-Conjugate

Unequal mixing of reagents


Unequal mixing of reagents

Improper calculation of standard curve dilutions

Incomplete washing


Plate not incubated long enough

Pipetting error

Unequal volumes added to wells

Pipetting error

Use diluent in the kit as blank value.Ensure accurate dilution of highest standardEnsure accurate completion of 2-fold dilution series

See washing procedures in the manualIncrease soaking time between washes

Ensure that wash apparatus is working correctlyEnsure adequate time of soaking

Ensure correct dilution

Ensure adequate mixing


Ensure adequate mixing

Check calculations, make new standard curve

Ensure that wash apparatus is working correctly


Increase Substrate Solution incubation time

Check calculations, make new standard curve

Check pipette function;Recalibrate the pipetter if necessary

Set duplicate in each assay

7.Poor Duplicates in ELISA experiment

Possible causes Solutions


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9.Very low readings across the plate in ELISA experiment

10.How to wash the ELISA plate?

8.Samples ' OD are too high, but standard curve looks fine

Possible causes

Possible causes

Solutions

Solutions

Incorrect wavelength

Samples contain determinant levels above the kit’s detection range

No plate sealers used

Insufficient incubation time

Incorrect dilutions of detection Ab/HRP-conjugate

Stop solution not added

Buffers contaminated

Incubation temperature vary wildly

The kit has lost its activity

Check filters/reader

Dilute samples and run again

No plate sealers

Increase incubation time

Adhere to the manual

Add stop solution to each well

Make fresh buffers

Check the incubator

Get a new kit

Correct washing of the wells is very crucial for a successful ELISA. Deionized or distilled water should be used when diluting the concentrated Wash Buffer.

Using multi-channel pipetteFirstly, check the strips in the plate so that they are firmly in the plate holder. Numbering the strips may be

useful in the event that strips become loose while decanting. Secondly, empty the liquid in the plate by decanting. Fill each well with the volume of Wash Buffer specified in the kit manual. Set a timer for the recommended time and let the Wash Buffer soak in the wells. Decant the wells by inverting the plate and rapping it against clean paper toweling. Repeat this process as directed in the kit manual. After the last decanting, remove any remaining Wash Buffer by inverting the plate and blotting it dry by rapping it firmly against clean paper toweling on a hard surface. Do not let the wells to sit dry. Proceed immediately to the next step in the kit manual.

Using manifold dispenser or autowasherEquip the dispenser or autowasher with an appropriate vacuum supply (refer to manufacturer’s guidelines).

Ensure that each cannulae is dispensing and aspirating properly. Set the volume of Wash Buffer in each well and soaking time for each washing. Then put the plate in. Ensure that no Wash Buffer is left in the wells after aspiration. Do not over-aspirate the wells by allowing the aspiration apparatus to sit in the wells after the aspiration.


Plate sealer reused Use a fresh plate sealer for each step

Insufficient washing Ensure sufficient washing

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11.Pipetting Tips for researchers

12. Calculation of ELISA results

The key to repeated accuracy in pipetting is a consistent technique. Consistent speed and smoothness is necessary while pipetting. Avoid sudden motions when drawing or dispensing liquid.

1. Check to be sure that the pipette tip is fitted properly on the end of the pipette and seated with adequate pressure

2. Change pipette tips between each sample or reagent addition. 3. Do not pipette a lower volume than the manufacturer’s suggestion. This will decrease accuracy and

reproducibility.4. Some liquids will have a tendency to cling to the inside or outside surfaces of the pipette tips. To avoid

variations in the volumes drawn or dispensed caused by this surface tension, pre-rinse the pipette tip with the sample or reagent. This will improve accuracy.

5. If the reagent to be pipetted is viscous, wait a moment after drawing the liquid to allow the volume to reach equilibrium before removing the pipette tip from the reservoir.

6. After drawing the liquid into the tip, remove the tip from the solution and wipe the outside of the tip with a lint-free tissue.

7. If an air bubble appears in the tip while drawing the liquid, dispense the liquid back into the container and re-pipette the liquid at a slower rate. If the air bubble appears a second time, discard the pipette tip and replace with a new tip.

8. If foaming occurs in the tip while drawing up the liquid, it may be necessary to hold the pipette at a slight angle and draw the liquid slower.

9. Use separate reservoirs for each reagent.

1. ELISA samples are always tested in duplicate or in triplicate, then average the absorbance values for each set of standards and samples. Duplicates should be within 20% of the mean.

2. Subtract the average value of zero standard OD (optical density).3. Standard curve.Create a standard curve by plotting the mean absorbance for each standard on the x-axis against the

concentration on the y-axis and draw a best fit curve through the points on the graph. We suggest the suitable software should be used for this, such as CurveExpert 1.3. The software is recommended that various methods (e.g. linear, semi-log, log/log, 4 parameter logistics) be tried to see which curve best fits the data. One way to determine if the curve is to backfit the standard curve O.D. values. Treat standards as unknowns and interpolate the O.D. values from the standard curve. They should be close to the expected value (+/- 10%). Use the curve that gives the best correlation (r) value and backfit.

Step by step as shown:

Washing the plate too rapidly or too slowly, incomplete washing or aspirating, and letting the wells to sit dry are all factors that will affect the precision of the ELISA.


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Step 1: Input information of the OD value and concentration of the standards

Step 2: Create and select a standard curve.

We recommend generating a standard curve for each set of samples assayed.


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Step 3: Calculate the concentration according to the curve

Input the OD value of the sample and click , then user can get the actual concentration of target protein in the sample.


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Or get the relevant equation

If the OD of the sample surpasses the upper limit of the standard curve, these samples should

be diluted before proceeding with the ELISA to obtain an accurate result. As for these samples, the

concentration obtained from the standard curve must be multiplied by the dilution factor when analyzing

the results.

Step 4: Samples that have an absorbance value falling out of the range of the standard curve


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Part of Published Articles


Cat.No Target Species Publications

E-EL-H0102E-EL-H0109

E-EL-H0091E-EL-H0102E-EL-H5413

E-EL-H2050

E-EL-H0105E-EL-H1064E-EL-H1580E-EL-H5574

Interleukin 6Tumor Necrosis FactorAlpha

Prostate Specific AntigenInterleukin 6Prostate specific membrane antigen

Pepsin

Interleukin 17Cytosolic PhospholipaseA2Prostaglandin E2Cytochrome C Oxidase Subunit II

Human

Human

Human

Human

Liao Y, Liu P, Guo F, et al. Oxidative burst of circulating neutrophils following traumatic brain injury in human.[J]. Plos One, 2013, 8(7):e68963. IF:3.234

Liu J, Lu C Y, Zhou H, et al. Flexible Gold Electrode Array for Multiplexed Immunoelectrochemical Measurement of Three Protein Biomarkers for Prostate Cancer[J]. Acs Applied Materials & Interfaces, 2014, 6(22):20137-20143. IF:6.723

Kang H S, Kim J K, Lee H J, et al. LRP1-dependent pepsin clearance induced by 2′-hydroxycinnamaldehyde attenuates breast cancer cell invasion[J]. International Journal of Biochemistry & Cell Biology, 2014, 53:15–23 IF:4.046

Sun Z, Wenbin L I, Hosipital B J S. Diet induced hyperhomocysteinemia increases intestinal inflammation in an animal model of colitis.[J]. Journal of Crohn S & Colitis, 2015(2):58-60. IF:6.234


Interleukin 4Interleukin 5Interleukin 13

Human

Li H T, Zhang T T, Chen Z G, et al. Intranasal administration of CpG oligodeoxynucleotides reduces lower airway inflammation in a murine model of combined allergic rhinitis and asthma syndrome[J]. International Immunopharmacology, 2015, 28(1):390-398. IF:2.427

E-EL-H0102

E-EL-H0180

E-EL-H0155

Interleukin 6

Interleukin 9

Cluster of Differentiation 42

Human

Human

Human

Tang J N, Shen L, Liu C L, et al. Plasma Levels of C1q/TNF- or Stable Angina Pectoris.[J]. American Journal of the Medical Sciences, 2015, 349(2):130-6. IF:1.389

Tuxun T, Apaer S, Ma H Z, et al. The Potential Role of Th9 Cell Related Cytokine and Transcription Factors in Patients with Hepatic Alveolar Echinococcosis[J]. Journal of Immunology Research, 2015, 2015(2):1-7. IF:2.93

Yuan B, Chen Q, Ding W Q, et al. Copolymer coatings consisting of 2-methacryloyloxyethyl phosphorylcholine and 3-methacryloxypropyl trimethoxysilane via ATRP to improve cellulose biocompatibility.[J]. Acs Applied Materials & Interfaces, 2012, 4(8):4031-9. IF:6.723

E-EL-H1163E-EL-H5475E-EL-H0183E-EL-H0196E-EL-H0873

ElastinC-telopeptide of type Ⅰ collagenN-Terminal Procollagen Ⅲ PropeptideProcollagen I C-terminal PropeptideCross Linked C-telopeptide of Type III Collagen

HumanLiu R, Chen L, Wei W, et al. Extracellular matrix turnover in coronary artery ectasia patients.[J]. Heart & Vessels, 2015:1-9. IF:2.065

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Cat.No Targets Species Publications

E-EL-H0375

E-EL-H1675

E-EL-0060E-EL-H1113E-EL-H5410E-EL-H5436


Fatty Acid Synthase

Proteoglycan 4

MalondialdehydeSuperoxide Dismutase 1,SolubleGlutathione Peroxidase 1Reactive OXyen Species

Interferon AlphaInterferon Beta

Human

Human

Human

Human

Qin W, Raatz S, Zhang K K, et al. Polyunsaturated Fatty Acid content may be increased in the milk of women with pregnancy-associated breast cancer.[J]. Journal of Human Lactation Official Journal of International Lactation Consultant Association, 2014, 30(4):420-4. IF:1.985

Guo H, Fang W, Li Y, et al. Up-regulation of proteoglycan 4 in temporomandibular osteoarthritic synovial cells by hyaluronic acid[J]. Journal of Oral Pathology & Medicine, 2014, 44(8):622–627. IF:1.926

Zhao, Jing, Xu, Shouzhu, Song, Fan. 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine-induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase[J]. Iubmb Life, 2014, 66(10):711-722. IF:3.143

Yang Q, Fu S, Wang J. Hepatitis C virus infection decreases the expression of Toll-like receptors 3 and 7 via upregulation of miR-758[J]. Archives of Virology, 2014, 159(11):2997-3003. IF:2.39

E-EL-H1478Parathyroid Hormone Related Protein

Human

Nordholm A, Rix M, Olgaard K, et al. Parathyroid hormone-related peptide plasma concentrations in patients on hemodialysis.[J]. Scandinavian Journal of Clinical & Laboratory Investigation, 2014, 74(3):206-12. IF:1.629

E-EL-H0190

E-EL-H0837

E-EL-H1382


E-EL-H1779E-EL-H1445E-EL-H1451E-EL-H2569

Complement Component 5a

Cross Linked C-telopeptide of Type II Collagen

Ribonuclease Inhibitor

Glucagon Like Peptide 1C-Peptide

Urokinase-Type Plasminogen ActivatorMatrix Metalloproteinase 2Matrix Metalloproteinase 9Vascular Endothelial Cell Growth Factor

Human

Human

Human

Human

Human

Wu Y, Wang Y, Qin F, et al. CD55 limits sensitivity to complement-dependent cytolysis triggered by heterologous expression of α-gal xenoantigen in colon tumor cells.[J]. Ajp Gastrointestinal & Liver Physiology, 2014, 306(12):G1056-64. IF:3.798

Park Y M, Su J K, Lee K J, et al. Detection of CTX-II in serum and urine to diagnose osteoarthritis by using a fluoro-microbeads guiding chip[J]. Biosensors & Bioelectronics, 2015, 67:192-199 IF:6.409

Spencer J D, Schwaderer A L, Eichler T, et al. An endogenous ribonuclease inhibitor regulates the antimicrobial activity of ribonuclease 7 in the human urinary tract[J]. Kidney International, 2014, 85(5):1179–1191. IF:8.563

Zaidan H K, Mohamed A H. Glucagon Like Peptide- 1 and C-peptide levels and their relationship with some physiological and biochemical variables of non-insulin-dependent diabetes mellitus (type 2) patients[J]. Australian Journal of Basic & Applied Sciences, 2014, 8(17):595-600. IF:0.786

Wang Z, Yin H, Wang T, et al. Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo.[J]. Bone, 2014, 45(6):2355-2364(10). IF:3.973

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16



E-EL-H2340E-EL-H0965E-EL-H2447E-EL-H5200E-EL-H1163E-CL-H1276



4-HydroxynonenalAlpha 1-AntitrypsinPeptidase Inhibitor 3, Skin DerivedLactoferrinElastinThymosin Beta 10

Toll-Like Receptor 4Toll-Like Receptor 2Mammalian Target of Rapamycin

Epinephrine/AdrenalineNoradrenaline/Norepinephrine

Human

Human

Human

Liu R, Chen L, Wu W, et al. Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients.[J]. Anatolian Journal of Cardiology, 2015, 16(1):23-8 IF:0.775

Kang M, Min K, Jang J, et al. Involvement of Immune Responses in the Efficacy of Cord Blood Cell Therapy for Cerebral Palsy.[J]. Journal of Back & Musculoskeletal Rehabilitation, 2015, 24(19): 2259-2268. IF:0.705

Tang C, Huang X, Kang F, et al. Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery.[J]. Mediators of Inflammation, 2015, 2015(6). IF:3.236

E-EL-H0344E-EL-0045E-EL-0047



Cerebral Dopamine Neurotrophic FactorEpinephrine/AdrenalineNoradrenaline/Norepinephrine

Procollagen I N-terminal PropeptideCross Linked C-telopeptide of Type I Collagen

Beta-EndorphinSuperoxide Dismutase, Soluble

Human

Human

Human

Li D, Wang Q, Zhang Y, et al. A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation.[J]. American Journal of Hypertension, 2016, 29(1):63-72. IF:2.852

Zhang K, Wang C, Chen Y, et al. Preservation of high-fat diet-induced femoral trabecular bone loss through genetic target of TNF-α[J]. Endocrine, 2015, 50(1):239-249. IF:3.878

Tang C, Chai X, Fang K, et al. I-gel Laryngeal Mask Airway Combined with Tracheal Intubation Attenuate Systemic Stress Response in Patients Undergoing Posterior Fossa Surgery[J]. Mediators of Inflammation, 2015, 2015(7). IF:3.236

E-EL-H1901 Nephrin Human

Petrica L, Vlad A, Gluhovschi G, et al. Proximal tubule dysfunction is associated with podocyte damage biomarkers nephrin and vascular endothelial growth factor in type 2 diabetes mellitus patients: a cross-sectional study.[J]. Plos One, 2014, 9(11):e112538-e112538. IF:3.234

E-EL-H1343 Osteocalcin Human

Ma W H, Liu Y J, Wang W, et al. Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication.[J]. Brazilian Journal of Medical & Biological Research, 2015, 48(4):299-307. IF:1.139

E-EL-H1274 Semaphorin 3A Human

Wang Z, Chen J, Zhang W, et al . Axon guidance molecule Semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma.[J]. Oncotarget, 2016, 7(5):6084-6062. IF:6.636

E-EL-H0108 Interferon Gamma Human

Rui-Xin Wu, Yang Y, Yuan Y, et al. Platelet lysate supports the in vitro, expansion of human periodontal ligament stem cells for cytotherapeutic use[J]. Journal of Tissue Engineering & Regenerative Medicine, 2016. IF:5.199

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17



E-EL-H2370

E-EL-R0014E-EL-R0016E-EL-R0022

E-EL-R0019E-EL-R0015


precursor of Prostate Specific Antigen

Interleukin 4Interleukin 10C-Reactive Protein

Tumor Necrosis Factor AlphaInterleukin 6

Interleukin 1 AlphaInterleukin 6

Human

Rat

Rat

Rat

Sun WG, Liang CZ, Zheng QC, et al. Influence of age on seven putative prostate tumor markers: a cohort study in Chinese men.[J]. Asian Journal of Andrology, 2016, 18:1-5. IF:2.596

Barquilla P C, Pagano E S, Jiménez-Ortega V, et al. Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats[J]. Journal of Pineal Research, 2014, 57(3):280-90. IF:9.6

Wang J, Yang H, Hu X, et al. Dobutamine-mediated heme oxygenase-1 induction via PI3K and p38 MAPK inhibits high mobility group box 1 protein release and attenuates rat myocardial ischemia/reperfusion injury in vivo[J]. Journal of Surgical Research, 2013, 183(2):509-16. IF:1.936

Chen J Y, Wu H, Li H, et al. Anti- inf lammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis[J]. International Immunopharmacology, 2015, 24(1):102-109. IF:2.472


E-EL-H1554E-EL-R0505

E-EL-R0011E-EL-R0014E-EL-R0016E-EL-R0019


Amyloid Beta Peptide 1-42Presenilin 1

High mobility group protein B1

Interleukin 1 AlphaInterleukin 4Interleukin 10Tumor Necrosis Factor Alpha

Interleukin 4Interleukin 6Interleukin 7Transforming Growth Factor β1

Human

HumanRat

Rat

Rat

Luciano R, Barraco G M, Muraca M, et al. Biomarkers of Alzheimer Disease, Insulin Resistance, and Obesity in Childhood.[J]. Pediatrics, 2015, 135(6):1074-81. IF:5.119

Wang X, Chu G, Yang Z, et al. Ethanol directly induced HMGB1 release through NOX2/NLRP1 inflammasome in neuronal cells[J]. Toxicology, 2015, 334:104-110. IF:3.621

Zúñigamuñoz A M, Guarner L V, Soriacastro E, et al. 17β Estradiol Modulates Perfusion Pressure and Expression of 5-LOX and CYP450 4A in the Isolated Kidney of Metabolic Syndrome Female Rats.[J]. International Journal of Endocrinology, 2015, 2015(6). IF:1.948

Dai M M, Wu H, Li H, et al. Effects and mechanisms of Geniposide on rats with adjuvant arthritis[J]. International Immunopharmacology, 2014, 20(1):46-53. IF:2.472

E-EL-RB0022 Alkaline Phosphatase Rabbit

Zhang W, Qin L, Li D, et al. The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing[J]. Materials Science & Engineering C, 2015, 46:10-15.Jiang J, Wan F, Yang J, et al. Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite.[J]. International Journal of Nanomedicine, 2014, 9(10):4569-80. IF:3.088,4.383

E-EL-Ch0026E-EL-Ch0848

Interferon GammaInterleukin 18

Chicken

Sun H, Wang L, Wang T, et al. Display of Eimeria tenella, EtMic2 protein on the surface of Saccharomyces cerevisiae, as a potential oral vaccine against chicken coccidiosis[J]. Vaccine, 2014, 32(16):1869-1876. IF:3.458

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E-EL-R0019

Tumor Necrosis Factor AlphaVascular Endothelial cell Growth FactorVascular Endothelial Growth Factor Receptor 1

LeptinInsulinAdiponectin

Tumor Necrosis Factor Alpha

Rat

Rat

Rat

Ruan Q, Zhao C, Ye Z, et al. Effect and possible mechanism of monocyte-derived VEGF on monocyte–endothelial cellular adhesion after electrical burns[J]. Burns Journal of the International Society for Burn Injuries, 2015, 41(4):825-832. IF:1.88

Wang Z, Chen J, Zhang W, et al . Axon guidance molecule Semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma.[J]. Oncotarget, 2016. IF:6.636

Ahmed L A, Darwish H A, Abdelsalam R M, et al. Role of Rho Kinase Inhibition in the Protective Effect of Fasudil and Simvastatin Against 3-Nitropropionic Acid-Induced Striatal Neurodegeneration and Mitochondrial Dysfunction in Rats[J]. Molecular Neurobiology, 2015:1-12. IF:5.137


C-Reactive ProteinInterleukin 6Tumor Necrosis Factor Alpha

Rat

Zhang Y, Imam M U, Ismail M, et al. Edible Bird’s Nest attenuates high fat diet-induced oxidative stress and inflammation via regulation of hepatic antioxidant and inflammatory genes[J]. Bmc Complementary & Alternative Medicine, 2014, 15(1):1-7. IF:2.02

E-EL-R2466

E-EL-R0582E-EL-R0023E-EL-0041

E-EL-R0023

E-EL-R2466E-EL-R0329E-EL-R0022E-EL-R0015E-EL-R0019

Insulin

LeptinInsulin8-Epi-Prostaglandin F2 Alpha

Insulin

InsulinAdiponectinC-Reactive ProteinInterleukin 6Tumor Necrosis Factor Alpha

Rat

Rat

Rat

Rat

Li H, Wang Y P, Zhang L N, et al. Perivascular adipose tissue-derived leptin promotes vascular smooth muscle cell phenotypic switching via p38 mitogen-activated protein kinase in metabolic syndrome rats.[J]. Experimental Biology & Medicine, 2014, 239(8):954-965. IF:2.165

Yida Z, Imam MU, Ismail M, et al. Edible Bird's Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats.[J]. Journal of Diabetes Research, 2015, 2015(9921):1-11. IF:2.164

Zhu S, Chen S, Yuan G, et al. Enhanced oral bioavailability of insulin using PLGA nanoparticles co-modified with cell-penetrating peptides and Engrailed secretion peptide (Sec)[J]. Drug Delivery, 2015:1-12. IF:2.558

Zhang Y, Imam M U, Ismail M, et al. High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats[J]. Journal of Biomedical Science, 2015, 22(1):1-10. IF:2.763


E-EL-R0566

Interleukin 10Interleukin 17Transforming Growth Factor β1Interferon Gamma

Interleukin 17

Rat

Rat

Lu X, Mccoy K S, Xu J, et al. Galectin-9 ameliorates respiratory syncytial virus-induced pulmonary immunopathology through regulating the balance between Th17 and regulatory T cells[J]. Virus Research, 2014, 195:162–171. IF:2.324

Alkuraishy H M, Al-Gareeb A I.Interleukin-17: A cardiac biomarker in estimation of cardioprotective effects of tacrolimus in doxorubicin-induced cardiotoxicity: animal model study.[J]. International Journal of Recent Scientific Research, 2015, 6(2):2769-2774. IF:5.971

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E-CL-R0018

Follicle Stimulating HormoneGonadotropin-Releasing HormoneLuteinizing Hormone

Tartrate-Resistant Acid Phosphatase 5bCross-linked C-Telopeptide of Type I Collagen

Bone Morphogenetic Protein 2Free Thyroxine

Epinephrine

Rat

Rat

Rat

Rat

Erkan L G, Altinbas B, Guvenc G, et al. Brain thromboxane A 2, via arachidonic acid cascade induces the hypothalamic–pituitary–gonadal axis activation in rats[J]. Autonomic Neuroscience Basic & Clinical, 2015, 189:50-55. IF:1.562

Kang W B, Deng Y T, Wang D S, et al. Osteoprotective effects of estrogen membrane receptor GPR30 in, ovariectomized rats[J]. Journal of Steroid Biochemistry & Molecular Biology, 2015, 154:237-244. IF:3.628

Ming W, Chai L, Zhao H, et al. Effects of nitrate on metamorphosis, thyroid and iodothyronine deiodinases expression in Bufo gargarizans, larvae[J]. Chemosphere, 2015, 139:402-409. IF:3.34

Aveseh M, Nikooie R, Sheibani V, et al. Endurance training increases brain lactate uptake during hypoglycemia by up regulation of brain lactate transporters[J]. Molecular & Cellular Endocrinology, 2014, 394(1–2):29-36. IF:4.405

E-EL-0065E-EL-R0113E-EL-R0884

EstradiolBone Alkaline PhosphataseSignal Transducer And Activator Of Transcription 5B

Rat

Wei Sun , Yuan-qin Wang, Qi Yan, et al. Effects of Er-Zhi-Wan on microarchitecture and regulation of Wnt/β-catenin signaling pathway in alveolar bone of ovariectomized rats.[J]. Journal of Huazhong University of Science & Technology, 2014, 34(1):114-119. IF:0.834


E-EL-R1235

E-EL-R0772


Amyloid Beta 1-40Amyloid Beta 1-42

Brain Derived Neurotrophic Factor

Procollagen I N-Terminal Peptide

Matrix Metalloproteinase 9Matrix Metalloproteinase 2

Rat

Rat

Rat

Rat

Gao H, Yan P, Zhang S. Chronic alpha-linolenic acid treatment alleviates age-associated neuropathology: roles of PERK/eIF2α signaling pathway.[J]. Brain Behavior & Immunity, 2015.Lin L, Yang S S, Chu J, et al. Region-specific expression of tau, amyloid-β protein precursor, and synaptic proteins at physiological condition or under endoplasmic reticulum stress in rats.[J]. Journal of Alzheimers Disease Jad, 2014, 41(4):1149-1163. IF:5.889,4.151

Chen Y, Zhang J, Deng M. Furin mediates brain-derived neurotrophic factor upregulation in cultured rat astrocytes exposed to oxygen–glucose deprivation[J]. Journal of Neuroscience Research, 2015, 93(1):189–194. IF:2.594

Hong Y J, Liang J G, Jiang N Y, et al. Vitamin K3 increased BMD at 1 and 2 months post-surgery and the maximum stress of the middle femur in the rat[J]. Nutrition Research, 2015, 35(2):155-161. IF:2.472

Liu Y, Zhong G, Zhao Z. Effect of chronic left ventricular unloading on myocardial remodeling: Multimodal assessment of two heterotopic heart transplantation techniques[J]. Journal of Heart & Lung Transplantation the Official Publication of the International Society for Heart Transplantation, 2014, 34(4):594–603. IF:6.65


OsteoprotegerinReceptor Activator of Nuclear Factor Kappa B Ligand

Rat

Zheng X, Wu G, Nie Y, et al. Electroacupuncture at the governor vessel and bladder meridian acupoints improves postmenopausal osteoporosis through osteoprotegerin/RANKL/RANK and Wnt/β-catenin signaling pathways.[J]. Experimental & Therapeutic Medicine, 2015, 10(2):221-224. IF:1.269

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E-EL-R1327


E-EL-R2466E-EL-R0582E-EL-M2416E-EL-M1272E-EL-M0160

E-EL-R0573

Creatine Kinase MB Isoenzyme

Matrix Metalloproteinase 13Collagen Type II Alpha 1

InsulinLeptinFibroblast Growth Factor Receptor 1Alanine TransaminaseAspartate Aminotransferase

Mucin 2

Rat

Rat

RatMouse

Rat

Hao G, Yu Y, Gu B, et al. Protective effects of berberine against doxorubicin-induced cardiotoxicity in rats by inhibiting metabolism of doxorubicin.[J]. Xenobiotica; the fate of foreign compounds in biological systems, 2015, 45(11):1024-1029. IF:2.199

Yang E, Zheng H, Peng H, et al. Lentivirus-induced knockdown of LRP1 induces osteoarthritic-like effects and increases susceptibility to apoptosis in chondrocytes via the nuclear factor-κB pathway[J]. Experimental & Therapeutic Medicine, 2015, 10(1):97-105. IF:1.269

Wu T, Qi X, Liu Y, et al. Dietary supplementation with purified mulberry ,（Morus australis, Poir）anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice[J]. Food Chemistry, 2013, 141(1):482-7. IF:3.391

S-J L, Jung Y H, Oh S Y, et al. Vibrio vulnificus VvpE inhibits mucin 2 expression by hypermethylation via lipid raft-mediated ROS signaling in intestinal epithelial cells[J]. Cell Death & Disease, 2015, 6(6):e1787. IF:5.014

E-EL-0065E-EL-0072E-EL-R0391

EstradiolTestosteroneFollicle Stimulating Hormone

Rat

Yan W J, Mu Y, Yu N, et al. Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet[J]. Journal of Assisted Reproduction & Genetics, 2015, 32(7):1097-1104. IF:1.718

E-EL-R0167


E-EL-R0710E-EL-R0965E-EL-R0966E-EL-R0582E-EL-R1079E-EL-0022

E-EL-R0633E-EL-M0049

Endothelin 1

Orexin AOrexin B

Oxidized Lowdensity LipoproteinThromboxanes B2Thromboxanes B3LeptinVon Willebrand FactorProstacyclin

Monocyte Chemotactic Protein 1Tumor Necrosis Factor Alpha

Rat

Rat

Rat

RatMouse

Kurt A, Tumkaya L, Turut H, et al. Efectos protectores de infliximab sobre el daño pulmonar inducido por metotrexato[J]. Archivos De Bronconeumología, 2015, 51(11):551-557. IF:1.823

Guo S J, Cui Y, Huang Z Z, et al. Orexin A-mediated AKT signaling in the dentate gyrus contributes to the acquisition, expression and reinstatement of morphine-induced conditioned place preference[J]. Addiction Biology, 2015, 139(10):210-215. IF:5.929

Zhang Y, Imam M U, Ismail M, et al. Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats[J]. Drug Design Development & Therapy, 2015, 9(9):3951-9.Yida Z, Imam MU, Ismail M, et al. N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats. Food & Nutrition Research. 2015;59:10.3402 IF:3.028,2.162

Cang D, Guo K, Zhao F. Dendritic cells enhance UHMWPE wear particle-induced osteoclast differentiation of macrophages[J]. Journal of Biomedical Materials Research Part A, 2015, 103(10):3349-3354. IF:3.369

E-EL-R0796 Prostate Specific Antigen RatAfriyie D K, Asare G A, Bugyei K, et al. Treatment of benign prostatic hyperplasia with Croton membranaceus, in an experimental animal model[J]. Journal of Ethnopharmacology, 2014, 157:90-98. IF:2.998

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E-EL-M2608E-EL-M2603

E-EL-M0048E-EL-M0043E-EL-M0047

E-EL-M0595

E-EL-M0676

E-EL-M1073

Free cholesterolTriglyceride

Interferon GammaInterleukin 4Interleukin 17

GremLin 1

High mobility group protein B1

Soluble Endothelial Protein C Receptor

Mouse

Mouse

Mouse

Mouse

Mouse

Wu T, Tang Q, Gao Z, et al. Blueberry and mulberry juice prevent obesity development in C57BL/6 mice.[J]. Plos One, 2013, 8(10):e77585. IF:3.234

Chen Z R, Zhang G B, Wang Y Q, et al. Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model[J]. Annals of Allergy Asthma & Immunology Official Publication of the American College of Allergy Asthma & Immunology, 2013, 111(4):276-281. IF:2.599

Siddique I, Curran S P, Ghayur A, et al. Gremlin promotes peritoneal membrane injury in an experimental mouse model and is associated with increased solute transport in peritoneal dialysis patients.[J]. American Journal of Pathology, 2014, 184(11):2976-84. IF:4.591

Li G, Chen M J, Wang C, et al. Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice[J]. International Immunopharmacology, 2014, 21(2):406-11. IF:2.472

Lagrange J, Li Z, Fassot C, et al. Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice.[J]. Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology, 2014, 28(5):2062-2072. IF:5.043

E-EL-M0048E-EL-M0026E-EL-M0049E-EL-M0043E-EL-M0046

Interferon GammaInterleukin 12Tumor Necrosis Factor AlphaInterleukin 4Interleukin 10

Mouse

A b d e l - H a f e e z E H , A h m a d A K , A b d e l g e l i l N H , e t a l . Immunopathological assessments of human Blastocystis, spp. in experimentally infected immunocompetent and immunosuppresed mice[J]. Parasitology Research, 2016:1-11. IF:2.098

E-EL-M0253


E-EL-M0511E-EL-0072

E-EL-M0372

Caveolin 1

High Density LipoproteinFree cholesterol

follicle-stimulating hormoneTestosterone

Beta Crosslaps

Mouse

Mouse

Mouse

Mouse

Xu W, Nan S, Bai W, et al. Caveolin-1 as a biomarker to predict therapeutic effect of low-frequency ultrasound combined with SonoVue on prostate cancer in nude mice model.[J]. Cancer Biomarkers, 2014, 14(4):279-86. IF:1.721Wu T, Tang Q, Yu Z, et al. Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice.[J]. International Journal of Food Sciences & Nutrition, 2014, 65(3):351-9. IF:1.206

Li H, He Y, Zhang H, et al. Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes[J]. Toxicology Letters, 2014, 225(3):433–444. IF:3.262

Zhang Y, Su J, Zhang W, et al. Discoidin domain receptor 2 facilitates prostate cancer bone metastasis via, regulating parathyroid hormone-related protein[J]. Biochimica Et Biophysica Acta, 2014, 1842(9):1350-1363. IF:3.836

E-EL-M0049E-EL-M0048E-EL-M0037E-EL-M0044E-EL-M0046E-EL-M0047

Tumor Necrosis Factor AlphaInterferon GammaInterleukin 1 BetaInterleukin 6Interleukin 10Interleukin 17

Mouse

Cheng J, Zhang L, Dai W, et al. Ghrelin ameliorates intestinal barrier dysfunction in experimental colitis by inhibiting the activation of nuclear factor-kappa B[J]. Biochemical & Biophysical Research Communications, 2015, 458(1):140-7. IF:2.297

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E-EL-M0349 Corticosterone Mouse

Li K, Liu F F, He C X, et al. Olfactory Deprivation Hastens Alzheimer-Like Pathologies in a Human Tau-Overexpressed Mouse Model via Activation of cdk5[J]. Molecular Neurobiology, 2014, 53(1):1-11. IF:5.137

E-EL-M1036S100 Calcium Binding Protein A12

MouseZhan Y, Zhang F, Shi Y, et al. Potential Effects of Calcium Binding Protein S100A12 on Severity Evaluation and Curative Effect of Severe Acute Pancreatitis[J]. Inflammation, 2014, 38(1):290-7. IF:1.973


E-EL-M0166

E-EL-M0693SAEP041SAEP021

E-EL-M2614

E-EL-M1215


Free Testosterone

Immunoglobulin G1Goat Anti-Mouse IgG FITC-Goat Anti-Mouse IgG

Insulin

Tumor Protein 53

Growth Regulated Oncogene AlphaChemokine C-X3-C-Motif Ligand 1

Mouse

Mouse

Mouse

Mouse

Mouse

Hrincius E R, Liedmann S, Finkelstein D, et al. NS1 mediated inhibition of c-Abl results in acute lung injury and priming for bacterial co-infections; insights into 1918 H1N1 pandemic?[J]. Journal of Infectious Diseases, 2014. IF:5.997

Li Y, Wu Y, Luo C, et al. Rewritable magnetic fluorescence-encoded microspheres: preparation, characterization, and recycling[J]. J.mater.chem.c, 2015, 3. IF:4.696

Song H, Lai J, Tang Q, et al. Mulberry ethanol extract attenuates hepatic steatosis and insulin resistance in high-fat diet-fed mice[J]. Nutrition Research, 2016. IF:2.472

Zhang D Y, Shen X Y, Qin R, et al. Effects of subchronic samarium exposure on the histopathological structure and apoptosis regulation in mouse testis[J]. Environmental Toxicology & Pharmacology, 2014, 37(2):505-512. IF:2.084

Wang H, Hong L J, Huang J Y, et al. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy[J]. Cell Research, 2015, 25(6). IF:12.413

E-EL-M0132 Apolipoprotein B Mouse

Smallwood T L, Gatti D M, Quizon P, et al. High-resolution genetic mapping in the diversity outbred mouse population identifies apobec1 as a candidate gene for atherosclerosis.[J]. G3 (Bethesda, Md.), 2014, 4(12):2353-2363. IF:2.511

E-EL-M0204

E-EL-M0511

E-CL-M0049

E-EL-M2614E-EL-M0002E-EL-M0039

Brain Natriuretic Peptide

follicle-stimulating hormone

Transforming Growth Factor β1

InsulinAdiponectinLeptin

Mouse

Mouse

Mouse

Mouse

Diané A, Nikolic N, Rudecki A P, et al. PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure.[J]. Journal of Endocrinology, 2014, 222(3):327-39. IF:3.718

Fan Y, Liu Y, Xue K, et al. Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier.[J]. Plos One, 2015, 10(4). IF:3.234

Li J, Zhao X, Liu X, et al. Disruption of TIM-4 in dendritic cell ameliorates hepatic warm IR injury through the induction of regulatory T cells[J]. Molecular Immunology, 2015, 66(2):117-125. IF:2.973

Chirag A. Patel, Jalaram H. Thakkar, Priyanka Patel, et al. LIPASE INHIBITORY AND ANTI-OBESITY EFFECT OF THE HYDRO ALCOHOLIC EXTRACT OF PINUS SYLVESTRIS BARK[J]. 2015, 4(4):1213-1229. IF:0.987

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E-EL-P0184 Brain Derived Neurotrophic Factor

Porcine

Yue C, Zheng Z, X i Z , e t a l . Lac to fer r in Promotes Ear ly Neurodevelopment and Cognition in Postnatal Piglets by Upregulating the BDNF Signaling Pathway and Polysialylation[J]. Molecular Neurobiology, 2014, 52(1):256-269. IF:5.137

EBLK0002 Biotin Labeling Mouse

Shao Y, Deng T, Zhang T, et al. FAM19A3, a novel secreted protein, modulates the microglia/macrophage polarization dynamics and ameliorates cerebral ischemia[J]. Febs Letters, 2015, 589(4):467-75.Chen C, Lv Y, Shi Q, et al. Preparation of human tau exon-2- and -10- specific monoclonal antibodies for the recognition of brain tau proteins in various mammals.[J]. International Journal of Molecular Medicine, 2015, 140(11):1889-1904. IF:3.169,2.088


E-EL-P0038Phosphodiesterase 4D, cAMP Specific

Porcine

Guan W, Liu W, Bao J, et al. Analysis and application of a neutralizing linear epitope on liable toxin B of enterotoxin Escherichia coli[J]. Applied Microbiology & Biotechnology, 2015, 99(14):5985-5996. IF:3.337

E-EL-0009 Folic Acid/Vitamin B9 Mitten Crab

Wei J, Yu N, Tian W, et al. Dietary vitamin B 12, requirement and its effect on non-specific immunity and disease resistance in juvenile Chinese mitten crab Eriocheir sinensis[J]. Aquaculture, 2014, 434:179-183.Wei J J, Zhang F, Tian W J, et al. Effects of dietary folic acid on growth, antioxidant capacity, non-specific immune response and disease resistance of juvenile Chinese mitten crab Eriocheir sinensis, (Milne-Edwards, 1853)[J]. Aquaculture Nutrition, 2015. IF:1.878,1.395

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