tips for elemental analysis of biological samples
TRANSCRIPT
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TIPS FOR ELEMENTAL ANALYSIS OF BIOLOGICAL SAMPLES
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Lead in blood and urine Pica in children Urban poisoning OSHA regulations
Hg, As, and Cd Insecticides Certain fish Water and air Toys
Forensic applications Intentional poisoning Weapon ID
Essential trace nutrients Se as antioxidant Cr for glucose metabolism Co for Vitamin B12
CLINICAL APPLICATIONS OF ICP
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CHALLENGES OF CLINICAL SAMPLES
Challenges:
Homogeneity Complex matrix Limited volume Speciation
Solutions:
Digestion; sonication; sample sizeNeb, torch, injectorMicro nebs, increased
transportHPLC-ICP-MS
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CHOOSING THE RIGHT NEBULIZER
ApplicationHigh dissolved solids
Particulates and high TDS
Limited volume
Very small sample
Nebulizer SeaSpray
VeeSpray
Low uptake SeaSpray (0.4ml/min)MicroMist
(0.05ml/min)
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TORCH SELECTION
Injector shape Aerosol dilution Outer tube
material
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PATENTED D-TORCH
Demountable outer tube
Demountable injector
Optional ceramic outer tube
Economical price
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QUARTZ VS. CERAMIC
6 HOURS OF RUNNING 10% NaCl
Quartz outer tube Ceramic outer tube
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WHY SPECIATION?
Bad form AsIII, AsV CrVI (CH3)Hg BrO3-
Not so badMMA, DMA CrIIIHgII Br-
Increasing requirement of speciation methods for toxicity limits (As, S, Cr).
Need for reliable HPLC and IC interface to your ICP.
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HPLC & IC INTERFACE KIT
• Resists blockage• Fast washout
• Zero dead volume• Minimizes peak broadening
The Answer: Glass Expansion LC Fittings Kit Quick and easy connection of your HPLC & IC to your ICP.
Compatible with any GE U-Series nebulizer.
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U-SERIES NEBULIZER(SeaSpray)
Made from constant bore thick walled tubing
Machined exterior provides proper aerodynamics
Heated and drawn glass capillary
Variation in wall thickness
Variation in bore ID
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ANALYSIS OF LIMITED SAMPLE VOLUMES
Biological applications Intracellular fluids Neonatal samples
Forensic applicationsWaste reduction applications Radioactive samples Toxic samples
Requires micro-flow nebulization Sensitivity and DL’s usually sacrificed
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EFFECT OF TEMPERATURE ON SENSITIVITY
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
Al 394.4
01
Cd 214.44
0
Cd 226.50
2
Co 238.89
2
Cr 267
.716
Cr 205
.560
Cu 327.39
3
Cu 324.75
2
Fe 238
.204
Ni 231
.604
Ni 221
.648
Pb 220
.353
Se 196
.026
V 292.46
4
Zn 206
.200
Zn 213
.857
Zn 202
.548
Rela
tive
Sens
itivi
ty
40C60C21C
PE Optima 2100DV
(20μL/min uptake)
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EFFECT OF TEMPERATURE ON DL
PE Optima 2100DV
0
2
4
6
8
10
12
Al 396
.153
Al 394
.401
Be 313
.107
Cd 214
.440
Cd 226
.502
Cr 267
.716
Cr 205
.560
Cu 327
.393
Cu 324
.752
Fe 238
.204
Mn 257
.610
Ni 231
.604
Ni 221
.648
Pb 220
.353
Se 196
.026
V 292.4
64
Zn 206
.200
Zn 213
.857
Zn 202
.548
Rel.
DL
2ml/min 21C.02ml/min 21C.02ml/min 60C
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FEATURES OF ISOMIST
Programmable from -10 to 60oC in 1oC increments
Maintains temperature to within 0.1oC
Compact design (7.5x4x4 inches)
100% self-contained (no external lines)
Incorporates Bluetooth® technology for clean wireless control (USB available)
Compatible with all ICP-OES and ICP-MS models
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CONSTANT TEMPERATURE BENEFITS
Higher accuracy Higher productivity Greater reproducibility Better experiments
(1mL/min sample uptake)