thursday posters bioinformatics iv, 001 - 021 · 2017. 1. 29. · page s122 58th asms conference on...

Page S122 58th ASMS Conference on Mass Spectrometry THURSDAY POSTERS

BIOINFORMATICS IV, 001 - 021 ThP 001 Quantitating Agilent Q-TOF Data Using MSQuant;

Joost W. Gouw1; Peter Mortensen2; Leonard J. Foster1;1University of British Columbia, Vancouver, Canada; 2University of Southern Denmark, Odense, Denmark

ThP 002 Automated Quantitative Analysis of LC-MS/MS Data with Isobaric Tags or Stable Isotope Labeling in MassMatrix Database Search Engine; Pang-Hung Hsu2; Liwen Zhang3; Michael A. Freitas3; Hua Xu 1;1Univ Illinois at Chicago, Chicago, IL; 2The Genomics Research Center, Taipei, Taiwan; 3Ohio State University, Columbus, OH

ThP 003 Statistical Validation of Peak Assignments and Ratio Estimations in Stable-Isotope Labeling Experiments;Alexey Nefedov; Rovshan Sadygov; University of Texas, Galveston, TX

ThP 004 PepPARQuant: An Alternative, Open-Source Approach to Processing 15N Quantitation Data; Phil Charles; Svenja Hester; Kathryn S Lilley; University of Cambridge, Cambridge, UK

ThP 005 ISOQUANT – A Java Based Tool for MySQL-Database Export and Integrated Evaluation of Data Independent (LC-MSE) Label-Free Quantitative Proteomics Data; Stefan Tenzer1; Jörg Kuharev1;Johannes Pc Vissers2, 3; Scott Geromanos2, 3; Hansjörg Schild1; 1UMC of the Johannes-Gutenberg-University Mainz, Mainz, Germany; 2Waters Corporation, Manchester, UK; 3Waters Corporation, Milford, MA

ThP 006 GProX - A Graphical Platform for Integrated Bioinformatics Analysis of Quantitative Proteomics Data; Kristoffer T. G. Rigbolt; Jens T. Vanselow; Blagoy Blagoev; Uni. of Southern Denmark, Odense, Denmark

ThP 007 Quantitative Proteome Analysis of the Regenerating Newt Heart by a Pulsed in vivo SILAC Approach;Mario Looso; Marcus Krueger; Thilo Borchardt; Anne Konzer; Thomas Braun; Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany

ThP 008 PVIEW2: A Comprehensive Open Source Software System for Isotope Labeled and Label-Free Protein Quantification; Zia Khan; Mona Singh; Leonid Kruglyak; Princeton University, Princeton, NJ

ThP 009 Increasing the Number of TMT-Quantifyable Proteins by Missing Value Imputation; Wiebke Timm;Dominic Winter; Marc Kirchner ; Judith A. Steen; Hanno Steen; Children's Hospital Boston / Harvard Medical, Boston, MA

ThP 010 Automatic Relative Quantification for High-Resolution LC/MS 16/18O Labeling Experiments;Anna Kreshuk1; Marc Kirchner 2; Bernhard Y Renard1;Dominic Winter2; Bernhard X Kausler1; Xinghua Lou1;Michael Hanselmann1; Judith A.J. Steen2; Hanno Steen2;Wolf D. Lehmann3; Fred A Hamprecht4;1University of Heidelberg, Heidelberg, Germany; 2Harvard Medical School/Children's Hospital Boston, Boston, MA; 3German Cancer Research Center, Heidelberg, Germany; 4Univ. of Heidelberg, Heidelberg, Germany

ThP 011 Using Archived Proteomics Data to Establish Virtual Immunoprecipitations, Reverse Immunoprecipitations, and Interaction Networks;Chengcheng Zhang; Jason Rogalski ; Daniel Evans; Ronald Beavis; Juergen Kast; University of British Columbia, Vancouver, BC

ThP 012 Quantitative Analysis on the Public Protein Prospector Site; Peter R Baker; Nicholas J Agard; A.L.

Burlingame; Robert Chalkley; University of California, San Francisco, CA

ThP 013 Effect of Normalization on Diagnostic Protein Detection in 2-200 kDa TOF Mass Spectra from Leukemia Serum; Maureen B. Tracy 2; Dariya Malyarenko2; Dennis Weaver1; Karl Kuschner2;Christine Bunai2; Eugene Tracy2; Dennis Manos2;William Cooke2; 1St. Leo University, Hampton, VA; 2College of William and Mary, Williamsburg, VA

ThP 014 Fragment Ion Repositories and Quantitative Protein Identifications; Johannes PC Vissers1; Stefan Tenzer2;Antoine H America3; Georgios Efstathiou4; Konstantinos Thalassinos4; Yishai Levin5; Andreaa Bodnari6; Jim Langridge1; Scott Geromanos1; 1Waters Corporation, Manchester, UK; 2University of Mainz, Mainz, Germany; 3PRI - Plant Research International, Wageningen UR,, Wageningen, Netherlands; 4University of Warwick, Coventry, UK; 5University of Cambridge, Cambridge, UK; 6University of Massachusetts Medical School,Worcester, MA

ThP 015 Variations in Human Serum Albumin Peptide Intensities and Identifications Originating from the Sample and LC-MS/MS Platform; Lisa E. Kilpatrick1;Amy-Joan L. Ham2; Daniel C. Liebler2; Yuri Mirokhin1;Jeri Roth1; Paul Rudnick1; Dmitrii V. Tchekhovskoi1;Corbin Whitwell2; Xinjian Yan1; Stephen Stein1; 1NIST,Gaithersburg, MD; 2Vanderbilt Univ. School of Medicine, Nashville, TN

ThP 016 Systematic Assessment of the Reproducibility of Relative Quantification Based on LC-MS with Replicates; Baozhen Shan1; Clark Chen2; Bin Ma3; 1BSI, Waterloo, Canada; 2Bioinformatics Solutions Inc., Waterloo, ON; 3University of Waterloo, Waterloo, ON

ThP 017 Quantitative Protein Expression Analysis of Glucose-Lactose Diauxie in Escherichia coli; Ekaterina Mostovenko; Hans Dalebout; Rene J. M. van Zeijl; André M. Deelder; Magnus Palmblad; Leiden University Medical Center, Leiden, Netherlands

ThP 018 Improved Label-Free Quantitation Algorithms to Analyze High Complex Biological Samples; KenAoshima 1, 3; Satoshi Tanaka1, 3; Hiroyuki Katayama1;Kentaro Matsuura1; Tatsuji Nakamura1; Hideki Watanabe1; Khin Than Myint1; Keith Wilcoxen2;Yoshiya Oda1,3; 1Eisai Co., Ltd, Tsukuba, Japan; 2Eisai Research Institute, Andover, MA; 3CREST, Japan Science and Technology, Saitama, Japan

ThP 019 New functionality for the Trans-Proteomic Pipeline: Tools for the Analysis of Proteomics Data; Luis Mendoza1; David Shteynberg1; Joseph Slagel1; Natalie Tasman2; Brian S Pratt2; Henry H. Lam 3; Jimmy Eng4;Alexey Nesvizhskii5; Andrew Keller1; Eric Deutsch1;Ruedi Aebersold6; Robert Moritz1; 1Institute for Systems Biology, Seattle, WA; 2Insilicos LLC, Seattle, WA; 3HongKong University of Science and Technology, Clear Water Bay, Hong Kong; 4University of Washington, Seattle, WA; 5University of Michigan, Ann Arbor, MI; 6ETH Zurich, Switzerland

ThP 020 MRMPath: A Web-Based Tool that Identifies Peptide Transitions for LC-MRM-MS Analysis and Its Application to Biological Pathways; Chiquito Crasto; Chandrahas Narne; Landon Wilson; Stephen Barnes; University of Alabama at Birmingham, Birmingham, AL

ThP 021 Evolutionary Architecture for High Performance Proteomics Research; Claudiu Farcas ; To-Ju Huang;Pavel Pevzner; Ingolf Krueger; Vineet Bafna; Nuno

58th ASMS Conference on Mass Spectrometry Page S123 THURSDAY POSTERS

Bandeira; Center for Computational Mass Spectrometry, UCSD, La Jolla, CAPROTEINS: RECOMBINANT, 022 - 043

ThP 022 A Rapid Method to Identify Sites of Low Level Glycation in Recombinant Antibodies by Isotopic Labeling with 13C6- reducing Sugars; Jennifer Zhang1; Taylor Zhang1; Lihua Jiang2; Daniel Hewitt1;YungFu Huang1; Yung-Hsiang Kao1; Viswanatham Katta1; 1Genentech Inc., South San Francisco, CA; 2Thermo Fisher Scientific, San Jose, CA

ThP 023 LC-MS Analysis of a Non-Natural Amino Acid Uptake and Its Incorporation Into a Recombinantly Expressed Protein in Pseudomonas fluorescens; Greg Cantin1; Diane Retallack1; Kat Woodard1; Tsotne Javahishvili2; Shailaja Srinagesh2; Trung Phuong2; Mark Shimazu2; Jeff Allen1; 1Pfenex Inc., San Diego, CA; 2Ambrx, Inc., La Jolla, CA

ThP 024 Investigation of Differential Methionine and Trytophan Oxidation in Antibody Complementarity Determining Region Sequences; Lowell J. Brady;Becky Scott; Capy Thwin; Julia Bach; Alison Wallace; Amy Guo; Lisa Connell-Crowley; Alain Balland; Amgen, Inc., Seattle, WA

ThP 025 Investigating Aspartate Isomerization of Sequence Motifs in Complementarity-Determining Regions of Monoclonal Antibodies Using High-Resolution Mass Spectrometry; Sean Mathewson; Boyan Zhang; Charlene Li; Vikas Sharma; Genentech, Inc., South San Francisco, CA

ThP 026 MS Analysis Reveals Differences in a Humanized mAb Protein Drug (Trastuzamab) and a Biosimilar Version; Martin Gilar1; Hongwei Xie1; Asish Chakraborty1; Deepalakshmi P. Dakshinamoorthy2;Weibin Chen 1; Ying-Qing Yu 1; St John Skilton1; Scott Berger1; 1Waters Corporation, Milford, MA; 2Waters India Pvt Ltd., Bangalore, India

ThP 027 Changes in Media Composition due to Expression of IgG1 in HEK 293 and CHO-S Cells; Peter Slade1;Defne Koch1; Daniela Hutanu1; David Judd2; Ming Lei1;Cheryl Bartel1; Steve Gorfien2; 1Life Technologies (Eugene), Eugene, OR; 2Life Technologies (Grand Island), Grand Island, NY

ThP 028 Determine Monoisotopic and Average Mass for Intact Proteins Using a high resolution Quadrupole Time-of-Flight Mass Spectrometry and Improved Deconvolution Algorithms; Ning Tang1; Xiaoling Wu1;Xiangdong Li1; Joe Roark1; Anthony Ferrige2; Keith Waddell1; 1Agilent Technologies, Santa Clara, CA; 2PPL, Isleham, UK

ThP 029 Quality Control of Intact Recombinant Proteins Using Sensitive High-Resolution Mass Spectrometry;Wolfgang Jabs; Dirk Wunderlich; Karsten Michelmann;Christian Albers; Carsten Baessmann; Bruker Daltonik GmbH, Bremen, Germany

ThP 030 MS as an Orthogonal Method to 2AB NP-HPLC for the Quantitative Determination of Glycans of Recombinant Monoclonal Antibodies; Wolfgang Wieder; Alfred Rupprechter; Werner Brunner; Mark Schiefermeier; Andreas Premstaller; Hansjoerg Toll; Sandoz GmbH, Kundl, Austria

ThP 031 Platform Independent Analysis System for Characterization of Chemical Liability in Biotherapeutics; Steven C. Pomerantz; Dariusz Janecki; Jennifer F. Nemeth; Centocor Research and Development, Radnor, PA

ThP 032 Analysis of N-linked Glycans from Recombinant and Human Plasma Derived Coagulation Factor IX Using HILIC LC/FLR/QTof MS; Ying Qing Yu 1; Weibin Chen1; Martin Gilar1; Cory Sutherland2; Anur Kumar2;Leland Paul2; 1Waters Corporation, Milford, MA; 2CMCICOS Biologics, Bothell, WA

ThP 033 Intact Protein Analysis Combining Micro-High Performance Liquid Chromatography and LTQ Orbitrap Mass Spectrometry; Remco Swart1; Jens Mohr2; Gunther Bohm3; Christian Huber2; 1Dionex Corporation, Amsterdam, Netherlands; 2Paris-LodronUniversity, Salzburg, Austria; 3Thermo-Fisher Scientific, Reinach, Switzerland

ThP 034 PRG 2010 Study: Tackling Unforeseen Problems in Otherwise ‘Straight-Forward’ Proteomics Analyses;David B. Friedman2; Tracy Andacht1; Maureen K. Bunger 3; Allis S. Chien4; David Hawke5; Jeroen Krijgsveld6; Robert Moritz7; Robert Settlage8; Chris Turck9; 1Centers for Disease Control and Prevention, Athens, GA; 2Vanderbilt University School of Medicine, Nashville, TN; 3RTI International, Research Triangle Park, NC; 4Stanford University, Stanford, CA; 5UT- M.D. Anderson Cancer Center, Houston, TX; 6EMBL Heidelberg, Heidelberg, Germany; 7InstituteFor Systems Biology, Seattle, WA; 8Virginia Bioinformatics Institute,Blacksburg, VA; 9Max Planck Institute, Munich, Germany

ThP 035 Mass Spectrometric Analysis of UV-A Light-Induced Cross-linking of the Lens Protein alphaB-crystallin with Antioxidant Supplementation; Kyle A. Floyd;David R. Stella; Ray Moore; Matthew B. Renfrow; Om P. Srivastava; Stephen Barnes; University of Alabama at Birmingham, Birmingham, AL

ThP 036 Characterization of Bacteriophage Derived Anti-staphylococcal Protein(P128) from Production to Purification Using Micro-Fluidic Based LC System Coupled to an Advanced QTOF-MS; Ravindra Gudihal1; Sundaram P.M2; Madhavi H.N2; Jiya Asrani2;1Agilent Technologies India Pvt. Ltd, Bangalore, India; 2GangaGen Biotechnologies Pvt. Ltd, Bangalore, India

ThP 037 ETD Mass Spectrometry for Determination of Disulfide Site and Scrambling Linkages: A Software Tool for Disulfide Mapping; Shiaw-Lin Wu1; Rovshan Sadygov3; Chen Li1; Tonya Second2; Zhiqi Hao2; David Horn2; Andreas F Huhmer2; Barry L. Karger1;1Northeastern University, Boston, MA; 2Thermo Fisher Scientific, San Jose, CA; 3University of Texas, Galveston, TX

ThP 038 Unrevealing Protein Modifications via Top-Down Fragmentation and Ion-mobility Time-of-flight Mass Spectrometry; Asish Chakraborty; Weibin Chen ; Jeff Mazzeo; Waters Corporation, Milford, MA

ThP 039 Fine Characterization of Therapeutic Antibodies for Batch Release Using Mass Spectrometry; Fabrice Cantais; Anicet Catrain; Géry Van Vyncht; Arnaud Delobel; Quality Assistance SA, Donstiennes, Belgium

ThP 040 Comparison of Detector Technologies for LC/QTof MSE Biotherapeutic Protein Peptide Mapping Studies; Jeff Mazzeo; Weibin Chen ; Scott Geromanos; Scott Berger; Waters Corporation, Milford, MA

ThP 041 Simultaneous Characterisation and Quantification of Hemaglutinin Proteins in a Vaccine Candidate with an LC-MS Assay; St John Skilton1; Joseph Rininger2;Weibin Chen1; Hongwei Xie1; Yinqing Yu1; 1Waters, Milford, MA; 2Protein Sciences, Meriden, CT


ThP 042 Quantitative Analysis of Host Cell Proteins in Biologics Derived from Different Cell Lines and Purification Schemes by a LC-MS Assay; Catalin Doneanu1; Manbir Labhan2; Alex Xenopoulos3; Holly Prentice3; Keith Fadgen 1; Weibin Chen 1; Martha Stapels1; St John Skilton1; William Haskins2; Jeff Mazzeo1; 1Waters, Milford, MA; 2University of Texas, San Antonio, TX; 3Millipore, Bedford, MA

ThP 043 Mapping the Surface Charge Pattern of a Recombinant Monoclonal Antibody Using Chemical Labeling and Mass Spectrometry; Liangyi Zhang;Charelene Li; Charles Eigenbrot; Boyan Zhang; Genentech Inc., South San Francisco, CA

PROTEIN CONFORMATION AND CHEMICAL CROSS-LINKING, 044 - 077

ThP 044 Design and Evaluation of a Temperature-Controlled Nano-ESI Source for the Studies of Thermal Denaturation of Proteins and Their Assemblies;Guanbo Wang; Rinat Abzalimov; Igor A. Kaltashov; University of Massachusetts, Amherst, MA

ThP 045 Comparison of Structure Stability between Biosimilar and Innovator Drugs Under Stressed Conditions by LC-MS analysis; Suli Liu; Haitao Jiang; Shiaw-Lin Wu; William Hancock; Barnett Institute,Northeastern University, Boston, MA

ThP 046 Resolving Alternative Structures of Intact Proteins by High-Field Asymmetric Ion Mobility Spectrometry; Julian Saba; Tonya Second; Thermo Fisher Scientific, San Jose, CA

ThP 047 Laser Spectroscopy of Trapped Green Fluorescent Protein Ions; Vladimir Frankevich; Konstantin Chingin; Robert J. Nieckarz; Renato Zenobi; ETH, Zurich, Switzerland

ThP 048 Optimizing Gaseous Protein Conformations for Top-Down Proteomics and Binding Energy Measurements; Sergio Castro1; Xianglei Kong 1; Steve Beu2; Kathrin Breuker3; Fred W. Mclafferty1; 1Cornell University, Ithaca, NY; 2S C Beu Consulting, Austin, TX; 3University of Innsbruck, Innsbruck, Austria

ThP 049 Immunological and Mass Spectrometric Study of Structural Changes in Ara h 2 in Roasted Peanuts;Jinxi Li 1; Kevin J. Shefcheck2; John H. Callahan2;Catherine Fenselau 1; 1University of Maryland, College Park, MD; 2FDA/CFSAN, College Park, MD

ThP 050 Secondary Structures of Soft- and Reactively Landed Multiply Charged Protein Ions; Qichi Hu; Peng Wang; Julia Laskin; Pacific Northwest National Laboratory, Richland, WA

ThP 051 Gaseous Protein Ion Conformations: A New Dimension of Structural Information from H/D Exchange Kinetics; Xianglei Kong 1; Sergio Castro1;Kathrin Breuker2; Fred W. Mclafferty1; 1CornellUniversity, Ithaca, NY; 2University of Innsbruck, Innsbruck, Austria

ThP 052 Ion Mobility and Cross-Linking Approaches to Structure Determination of Protein Complexes by Mass Spectrometry; Antonio Calabrese; Jingjia He; Tara Pukala; University of Adelaide, Adelaide, Australia

ThP 053 A Mass Spectrometric View at the in Transcription Involved Protein Complexes RNA Polymerase and Mediator; Kristina Lorenzen1; Sebastian Geiger2;Tobias Koschubs2; Patrick Cramer2; Albert J.R. Heck1;1University Utrecht, Utrecht, Netherlands; 2Ludwig-Maximilians-Universität München, München, Germany

ThP 054 Development of Ion Mobility-Mass Spectrometry as a High-throughput Approach for Structural Genomics;

Yueyang Zhong; Suk-Joon Hyung; Brandon Ruotolo; University of Michigan, Ann Arbor, MI

ThP 055 Conformational Changes Associated with the Removal of Zinc Ions from Proteins in a Cyanobacterial Zn2+ Homeostatic System; Frances D. L. Kondrat; Charlotte A. Scarff; Oksana I. Leszczyszyn ; Gregory R. Kowald; Claudia A. Blindauer; James H. Scrivens; University of Warwick, Coventry, UK

ThP 056 Probing Transferrin-Metal Interactions by Means of Traveling-Wave Ion Mobility Mass Spectrometry;Charlotte A. Scarff; Frances D. L. Kondrat; Claire Booyjzsen; Arindam Mukherjee; Peter J. Sadler; James H. Scrivens; University of Warwick, Coventry, UK

ThP 057 A Critical Comparison of Positively and Negatively Charged Peptide Ion Structures Using Ion Mobility-Mass Spectrometry; Nkiruka Arinze; Michal Kliman; Larissa S. Fenn; Sevugarajan Sundarapandian; Cody Goodwin; John A. Mclean; Vanderbilt University, Nashville, TN

ThP 058 Oxidative Footprinting and Hydrogen/Deuterium Exchange Mass Spectrometry for Epitope Mapping of Cancer Therapeutic Immunotoxins; Erin D. Hopper1; Masanori Onda2; Johanna Hansen2; Ira Pastan2;Kenneth B. Tomer1; 1NIEHS, Research Triangle Park, NC; 2NCI, Bethesda, MD

ThP 059 Submillisecond Time-Resolved Temperature-Jump Induces Protein Conformational Change as Probed by Fast Photochemical Oxidation of Proteins (FPOP);Jiawei Chen; Don L. Rempel; Michael L. Gross; Washington University, St. Louis, MO

ThP 060 Collision Cross Sections of Monomer, Dimer and Tetramer Gas-phase Ions from Human Hemoglobin;Yang Kang; Peran Terrier ; Donald J. Douglas; University of British Columbia, Vancouver, BC

ThP 061 Development of a New Flow Cell System for Short-Duration Oxidative Protein Footprinting Using Ultraviolet Laser Flash Photolysis; Jason S. Sampson 1; Jinglan Wang1; James G. Smedley, III2; Kenneth B. Tomer1; 1NIEHS, Research Triangle Park, NC; 2KBIBiopharma, Durham, NC

ThP 062 Structural Analysis of Neurodegenerative Disease Protein Aprataxin by Chemical Surface Mapping and Mass Spectrometry; Jinglan Wang; R. Scott Williams; Kenneth B. Tomer; Leesa Deterding; NIEHS, Research Triangle Park, NC

ThP 063 pH Dependence the Chemical Labeling of Thermus thermophilus HB8 Ribosomes; William Running;James P. Reilly; Indiana University, Bloomington, IN

ThP 064 Covalent Labeling with Mass Spectrometry Identifies Subtle Structural Changes Associated with Protein Binding to Different Divalent Metal Ions; Vanessa Leah Mendoza; Jia Dong; Richard Vachet; University of Massachusetts, Amherst, MA

ThP 065 Probing of Functional Regions on Coagulation Factor VIII; Esther Bloem1; Henriet Meems1; Jacqueline Klein Gebbinck1; Koen Mertens1, 2; Alexander B. Meijer1, 2;1Department of Plasma Proteins, Sanquin Research, Amsterdam, The Netherlands; 2Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands

ThP 066 Structural Allostery and Binding Interface of Actin-Human Adenovirus Proteinase Complex; Rhijuta D'Mello1; Sayan Gupta1; Vito Graziano2; William McGrath2; Mark Chance1; Walter Mangel2; 1Case Western Reserve University, Cleveland, OH; 2Biology Department- Brookhaven National Laboratory, Upton, NY


ThP 067 Conformational Mapping of Proteins Using an IR-Chromogenic Crosslinker and Infrared Multiphoton Dissociation; Lisa A Vasicek; Scott Robotham; Jennifer Brodbelt; The University of Texas, Austin, TX

ThP 068 Characterization of Protein Structure Using Electron Transfer Dissociative Chemical Cross-Linker and Tandem Mass Spectrometry; Myles Gardner; Lisa Vasicek; Colin Kubarych; Eric Anslyn; Jennifer Brodbelt; The University of Texas, Austin, TX

ThP 069 How to Efficiently Find a Needle in a Haystack: Method Optimization for Shotgun Chemical Cross-Linking Mass Spectrometry; Adam Klein1, 2; Young-Jin Lee1, 2; 1Iowa State University, Ames, IA; 2US-DOEAmes Laboratory, Ames, Iowa

ThP 070 Human Regulatory Protein 14-3-3 and Its Interactome by MS3D and H/D Exchange Techniques; Katerina Pavlaskova1, 2; Hynek Mrazek1, 2;Petr Man1; Vladimir Havlicek1; Tomas Obsil3; Petr Novak1; Miroslav Sulc1, 2; 1Institute of Microbiology,v.v.i., Prague, Czech Republic; 2Faculty of Science, Charles University, Prague, Czech Republic; 3Institute of Physiology, v.v.i., Prague, Czech Republic

ThP 071 Mapping the Spatial Assembly of Enzymatic Complex Using MS3D Technique; Petr Pompach1, 2;Petr Man1, 2; Karel Bezouska1, 2; Vladimir Havlicek1, 3;Petr Novak1, 2; 1Institute of Microbiology, Prague, Czech Republic; 2Charles University,Prague, Prague, Czech Republic; 3Palacky University, Olomouc, Czech Republic

ThP 072 Chemical Cross-linking and H/D Exchange Combined with Mass Spectrometry: A Tool to Validate and Refine Protein Crystal Structure;Daniel Rozbesky1, 2; Petr Pompach1, 2; Petr Man1, 2;Vladimir Havlicek1, 3; Karel Bezouska1, 2; Petr Novak1, 2;1Institute of Microbiology, Prague, Czech Republic; 2Charles University, Prague, Czech Republic; 3Palacky University, Olomouc, Czech Republic

ThP 073 Tandem Affinity Selective Enrichment of Inter-Peptide Crosslinks for Complex Structural Proteomics Crosslinking Applications; Evgeniy V. Petrotchenko; Christoph H. Borchers; UVic-GBCProteomics Centre, Victoria, Canada

ThP 074 Novel Mass Defect-Labeled Mass-Spectrometry Identifiable Cross-linker Applied to the 34kDa-Actin Protein System; Lisabeth Hoffman1; Paul Griffin1;Marcus Fechheimer1; Evgeniy Petrotchenko2; Christoph Borchers2; Jon Amster1; 1University of Georgia, Athens, GA; 2UVic-GBC Proteomics Centre, Victoria, BC

ThP 075 Characterization of Structure and Interactions of Transthyretin Using Chemical Cross-Linking and Top-Down Mass Spectrometry; Xiaobin Xu1;Giuseppe Infusini 2; Nancy Leymarie 2; David H. Perlman2; Mark E. Mccomb2; Catherine E. Costello2;1Boston University, Boston, MA; 2Boston University School of Medicine, Boston, MA

ThP 076 An Isotopically-coded CID-Cleavable Biotinylated Crosslinker CBDPS; Evgeniy V. Petrotchenko; Jason J. Serpa; Christoph H. Borchers; UVic-GBC Proteomics Centre, Victoria, Canada

ThP 077 A New Category of Protein Crosslinkers for Protein Complexes; Billy Clifford-Nunn; Janine R. Maddock; H.D. Hollis Showalter; Philip C. Andrews; University of Michigan, Ann Arbor, MI

PROTEOMICS: PTM DETERMINATION, 078 - 100 ThP 078 Spot the Difference: Proteomic Epigenetic Reference

Maps for Discovery of Differential Histone

Modifications among Disparate Cellular States; Jacob D. Jaffe; Namrata Udeshi; Sally Peach; D. R. Mani; Steven A. Carr; The Broad Institute, Cambridge, MA

ThP 079 Probing the Global PTM Profile of Histones H4 and H3: Label Dependent Shot-Gun Proteomics; Elisabeth Hersman; Christine Jelinek; Junbiao Dai; Dwella Moton Nelson; Rocio Montes de Oca; Katherine Wilson; Jef Boeke; Robert Cotter; Johns Hopkins School of Medicine, Baltimore, MD

ThP 080 Quantitative Cross-Species Comparison of Global Histone H3 Modification States between Drosophilamelanogaster and Homo sapiens Using SILAC; Sally E. Peach1; Yuri B. Schwartz2; Yincenzo Pirrotta2; Steven A. Carr1; Jacob D. Jaffe1; 1The Broad Institute, Cambridge, MA; 2Rutgers Univ. Dept. of Mol. Biol. & Bioch., Piscataway, NJ

ThP 081 Improved Methods for Comprehensive Assignment of Epigenetic Modification Configurations of Core Histone Proteins by High-Resolution ETD and HCD Mass Spectrometry; Namrata Udeshi; Sally Peach; Steven A. Carr; Jacob D. Jaffe; The Broad Institute, Cambridge, MA

ThP 082 Mapping of Lysine Methylation and Acetylation in Core Histones in Neurospora crassa; Lei Xiong; Fan Zhang; Yinsheng Wang; Chemsitry Department, UCRiverside, Riverside, CA

ThP 083 Insight into the Mechanism of Polycomb Mediated Gene Silencing by Use of Quantitative Mass Spectrometry Analyses of Histone Modifications;Shannon M Eliuk1; Feixia Chu2; Barbara Panning1; Al Burlingame1; 1University of California San Francisco, San Francisco, CA; 2University of New Hampshire, Durham, NH

ThP 084 Top-Down Characterization of Core Histones Using an Online Two-Dimensional Nanocapillary Liquid Chromatography System; Zhixin Tian; Rui Zhao; Nikola Toli ; Ronald J. Moore; David L. Stenoien; Errol W. Robinson; Richard D. Smith; Ljiljana Paša-Toli ;Pacific Northwest National Laboratory, Richland, WA

ThP 085 Combining Post-Translational Modification Specific Antibodies and Mass Spectrometry to Study PTMs on Histone H4; Katherine L. Stamper 1; Robert J. Cotter2; 1Johns Hopkins University, Baltimore, MD; 2Middle Atlantic MS Laboratory, Baltimore, MD

ThP 086 An Informatics Pipeline for the Detection of Protein Modifications from Proteomics Mass Spectrometry Data; James Wright; Jyoti Choudhary; Wellcome Trust Sanger Institute, Hinxton, UK

ThP 087 On-Line, Salt-Free, Three-Dimensional Nanoflow-LCMS for Comprehensive Epitope-Mining; Ad de Jong; Geert Mommen; Netherlands Vaccin Institute, Bilthoven, The Netherlands

ThP 088 Effect of Mitochondrial Superoxide Dismutase (SOD2) RNA Interference Knockdown on Mitochondrial Protein Carbonylation in Drosophilamelanogaster; David Simpson; Ian Martin; Mike Grotewiel; Scott Gronert; Virginia Commonwealth University, Richmond, VA

ThP 089 The First Global Screening of Protein Substrates Bearing Protein-bound 3,4-dihydroxyphenylalanine in E. coli and Human Mitochondria; Sang Kyu Lee;Yue Chen; Hao Luo; Yingming Zhao; Ben May Department for Cancer Research, The Univsersity of Chicago, Chicago, IL

ThP 090 Modification and Inactivation of Mitochondrial Aconitase by 4-Hydroxy-2-Nonenal; Qingyuan Liu 1;


Scott Gronert2; 1Virginia Commonwealth University, Richmond, VA; 2Virginia Commonwealth Uni, Richmond, VA

ThP 091 Site-Specific Characterisation of Hydroxyproline Residues in Collagen by MALDI-QIT-TOF MSn Analysis; Helen Montgomery1; Nitin Rustogi2; Koichi Tanaka 3; Chris Sutton2; 1Shimadzu, Koichi Tanaka MS Research laboratory, Manchester, UK; 2Institute of Cancer Therapeutics, Univ.of Bradford, Bradford, UK; 3Shimadzu Corporation, Kyoto, Japan

ThP 092 Identification and Assignment of Protein Citrullination Sites after Chemical Derivatization Using Alternating CID and ETD on a Spherical Ion Trap; Maria Stensland2; Anders Holm2; Burkhard Fleckenstein2; Andrea Kiehne1; Arnd Ingendoh1; Andrea Schneider1; 1Bruker Daltonics, Bremen, Germany; 2Centre for Immune Regulation, University of Oslo, Oslo, Norway

ThP 093 Integration of Top-Down and Bottom-Up Proteomic Approaches: Applications of Protein/Peptide Identifications by On-Line Post-Column Digestions, Enzymatic Reactions, and High-Resolution MS;Matthew A. Rosenow; Tony Tegeler; Jian Liu; Linda Nagore; Ashoka Polpitiya; Konstantinos Petritis; Translational Genomics Research Institute, Phoenix, AZ

ThP 094 Comprehensive Identification of Nitroxyl (HNO)-reactive Cysteines in Human Platelet Proteins by Quantitative Mass Spectrometry; Liwen Lin1, 2;Juergen Kast 1, 2; 1Dept. of Chemistry, University of British Columbia, Vancouver, Canada; 2Biomedical Research Centre, Vancouver, Canada

ThP 095 Identification of Post Translational Modification Sites in Dynamin-Related Protein 1; Weitao Jia; Laura L. Lackner; Marjin G. Ford; Jodi Nunnari; Julie A. Leary; Dept. of Molecular and Cellular Biology, U.C.Davis, Davis, CA

ThP 096 Characterization of Oxidative Stress-Induced Protein Nitration in the Mitochondrial -ketoglutarateDehydrogenase Complex; Haiqiang Yu1; Qingli Shi2;Hui Xu2; Haiteng Deng1; Gary Gibson2; 1The Rockefeller University, New York, NY; 2Weill Med College of Cornell University, White Plains, New York

ThP 097 Identification of Animal Species by the MALDI-MS of Collagen in Animal Glues Used in Archaeological Materials; Takashi Nakazawa1; Atsuko Miyaji1; Yoshiki Matsuo1; Yuzo Yamazaki2; 1Nara Women's University, Nara, Japan; 2Shimadzu Corporation, Kyoto, Japan

ThP 098 Comparing the Efficiency of Hydrazide Labels in the Study of Protein Carbonylation: Human Serum Albumin as a Test System; Zafer Ugur; Scott Gronert; Virginia Commonwealth Uni., Richmond, VA

ThP 099 Identification of Novel Interaction Partners for Chromo, Tudor and MBT Domains by Heavy-Methyl SILAC-Based Proteomics; Sina Pleiner; Gerhard Mittler; Max-Planck-Institute of Immunobiology, Freiburg, Germany

ThP 100 Comparative Redox Proteomics to Target Oxidatively Modified Cysteines in Fusariumgraminearum Using Liquid Chromatography/Mass Spectrometry; Manisha Joshi1; Christof Rampitsch2; R Subramaniam3; 1Department of Biochemistry and Medical Genetics, Winnipeg, Canada; 2Agriculture and Agri-Food Canada, CRC, Winnipeg, Canada; 3Agriculture and Agri-Food Canada, Ottawa, Canada

PROTEOMICS: NEW APPROACHES III, 101 - 130 ThP 101 Advances in Capillary Liquid Chromatography for

High-Throughput Top Down Proteomics; Adam D. Catherman1; John C. Tran1; Lee Sawdey2; Kenneth R. Durbin1; Adaikkalam Vellaichamy1; Gary Valaskovic2;Neil L. Kelleher1; 1University of Illinois, Urbana, IL; 2New Objective, Inc., Woburn, MA

ThP 102 Proteomic Profiling of Complex Samples Using Multidimensional Protein and Peptide Separation Strategy and Accurate Mass Mass Spectrometry;Vadiraja B. Bhat; Haiying Chen; James Martosella; Agilent Technologies, Wilmington, DE

ThP 103 Mass Spectrometric Analysis of the Efficiency of Displacement Chromatography for the Chromatographic Separation of Complex Protein Mixtures; Marcel Kwiatkowski 1; Moritz Wagner 3;Robert Ahrends 1; Björn Lichtner1; Andreas Bertsch 2;Oliver Kohlbacher 2; Maria Trusch 1; Hartmut Schlüter1;1University Medical Center Hamburg-Eppendorf, Hamburg, Germany; 2University of Tübingen, Wilhelm Schickard Institut, Tübingen, Germany; 3Agilent Technologies, Waldbronn, Germany

ThP 104 Segmented 1D-nanoLC Combined with CID/ETD for Increased Sequence Coverage in Biomarker Discovery; Thomas Hagedorn; Andrea Kiehne; Markus Lubeck; Carsten Baessmann; Arnd Ingendoh; Bruker Daltonik GmbH, Bremen, Germany

ThP 105 Online RP-RP Two Dimensional Liquid Chromatography for Shotgun Proteomics Using Peak-Parking and Partial-Loop Injection; Maggie P. Y. Lam1; S. O. Siu1; Edward Lau1; J. Zhang1; Simon M. Y. Lee2; Ivan K. Chu1; 1The University of Hong Kong, Hong Kong, China; 2University of Macau, Macau, China

ThP 106 Integrated Microfluidic nanoESI-MS Platform for Sample Preparation and Analysis of Single Cells;Nitin Agrawal; Ryan Kelly; Xuefei Sun; Richard D. Smith; Pacific Northwest National Laboratory, Richland, WA

ThP 107 Investigating Chromatographic and Mass Spectrometric Resolution and Accuracy on Relative and Absolute Quantification of Proteins Associated with Cellular Hypoxia; Joanne B. Connolly 1; Chris Hughes 1; Thérèse Mckenna1; Jim Langridge1; Susan Critchlow2; Rachel Rowlinson2; Lorna Tate2; Tom Dunkley2; 1Waters Manchester UK, Manchester, UK; 2AstraZeneca, Alderley Park, UK

ThP 108 Preparation of a New Type of Packed Capillary Column with a Monolithic Emitter and Its Application in Proteomic Studies; Yangjun Zhang1;Jingxin Xie2; Weijie Qin1; Xiaohong Qian1; 1BeijingInstitute of Radiation Medicine, Beijing, China; 2Shenyang Pharmaceutical University, Shenyang, China

ThP 109 Peptide Identities Can Inform Peak Detection Parameters for 2D MS/MS Data; Devin Lee Drew1;Roumiana Alexandrova2; Nadim Jessani1; Jacqueline Mason2; Xunyi Luo2; James G. Pan2; Faith Au Yeung2;Theo Goh2; 1Genedata, Inc., San Francisco, CA; 2Campbell Family Inst. for Breast Cancer Research, Toronto, Canada

ThP 110 Optimal and Robust Analysis of High-Mass-Accuracy Orbitrap Datasets Using MaxQuant and ProteinPilot Search Algorithms; Rongxiao Sa1;Leeann Higgins 1; Sean L. Seymour2; Sricharan Bandhakavi1; Edgar A. Arriaga1; Tim Griffin1; Pratik Jagtap3; 1University of Minnesota, Minneapolis, MN;


2AB SCIEX, Foster City, CA; 3MinnesotaSupercomputing Institute, UMN, Minneapolis, MN

ThP 111 Rapid Approaches for Data-independent Tandem Mass Spectrometry; Scott A. Shaffer; Alexandre Panchaud ; Shouhua Chen; David R. Goodlett; University of Washington, Seattle, WA

ThP 112 Data-Independent Acquisition for Effective Proteome Profiling on Three Different Linear Ion Trap Configurations; Jesse D. Canterbury; Scott A. Shaffer; Jarrett Egertson; David R. Goodlett ; Michael J. Maccoss; University of Washington, Seattle, WA

ThP 113 Comparative HPLC and Ultra High Performance nanoLC-MS/MS analysis of iTRAQ Labeled Cancer Tissue with Exclusion List Generation; X. Simon Wang1; Udo. H Verkerk1; Leroi. V DeSouza1; Remco. V Soest2; K. W. Michael Siu1; 1York University, Toronto, Canada; 2Eksigent Technologies, Inc, Dublin, California

ThP 114 Separation Free Proteomics: Coupling of a microfluidic Proteolytic Reactor to Electrospray Ionization Mass Spectrometry to Analyze Protein Mixtures; Karan Hingorani; Derek Wilson; York University, Toronto, Canada

ThP 115 Rapid High-Resolution Reversed-Phase LC-MS Analyses of Peptides and Tryptic Digests Using New Fused-Core Particle Columns; Barry Boyes1, 2; Darryl Johnson2; Stephanie Schuster1; Jack Kirkland1; Ron Orlando2; 1Advanced Materials Technology Inc, Wilmington, DE; 2University of Georgia, Athens, GA

ThP 116 Protein Prefractionation Ahead of 2DLC-MSMS Enhanced Neuroproteome Coverage and Provides Biologically Relevant Spatial Information; Miranda K. Landis; Brian F. Fuller; Andrew K. Ottens; Virginia Commonwealth University, Richmond, VA

ThP 117 Hydrophilic Interaction Chromatography Combined with Reversed Phase LC-MS Benefits Depth and Quality of Proteome Analysis Compared With Other Orthogonal Techniques; Lynn Spruce 1; Jessica Lee1;Hua Ding1; Jason Wojcechowskyj2; Steven H. Seeholzer1; 1Children's Hospital of Philadelphia Research Insti, Philadelphia, PA; 2University of Pennsylvania, Philadelphia, PA

ThP 118 Microfluidic Distributor/Reversed Phase Monolithic Column Array Devices for Fraction Collection and Analysis of Peptide Mixtures; Jian Liu; Daniel Higbee; Daniel R. Knapp; Medical University of SC, Charleston, SC

ThP 119 1D Quantification with 2D Identification of Proteins in Label-Free Proteomics: The Best of Both Worlds;Yishai Levin1; Cindy Chepanoske2; Tim Bonnert2;Sabine Bahn1; 1University of Cambridge, Cambridge, UK; 2Ceiba Solutions, Cambridge, MA

ThP 120 First Light: Three Dimensional Separations Drive a Realization of High-Throughput Top Down Proteomics; John C. Tran; Leonid Zamdborg; Kenneth R. Durbin; Ji Eun Lee ; Mingxi Li; John F. Kellie ; Adaikkalam Vellaichamy; Adam D. Catherman; Cong Wu; Dorothy Ahlf; Paul Thomas; Neil L. Kelleher; University of Illinois at Urbana-Champaign, Urbana, IL

ThP 121 Ultrafast Capillary Electrophoresis MS/MS Analysis of Peptide Mixtures and Protein Digests in about a Minute; Mehdi Moini; Ben Martinez; Texas State University, San Marcos, TX

ThP 122 Development of a Proteomics-on-a-chip Device with Fast MALDI Readout; Jeonghoon Lee; Jon M. Beusse; Steven A. Soper; Kermit K. Murray; Louisiana State University, Baton Rouge, LA

ThP 123 Comprehensive Identification of Human Kinases by Enhanced Capture Compound™ Mass Spectrometry Using SCX-RP-2D-LC-MS/MS; Olivia Graebner; Mirko Glinski; Jenny J. Fischer; Jan Ungewiß; Simon Michaelis; Christian Dalhoff; Friedrich Kroll; Michael Sefkow; Mathias Dreger; Hubert Köster; caprotecbioanalytics GmbH, Berlin, Germany

ThP 124 Investigating the Neurospora crassa Proteome Using a Dual Stage pI-Based Fractionation Scheme and LC-MS/MS Analysis; Stephanie M. Cologna; William K. Russell; Ana Victoria Suescun; Gyula Vigh; Rodolfo Aramayo; David H. Russell; Texas A&M University, College Station, TX

ThP 125 One Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils Entire Proteome Expressed in Escherichia coli cells; Mio Iwasaki 1; Shohei Miwa3;Oleg V. Krokhin2; Masaru Tomita1; Nobuo Tanaka3;Yasushi Ishihama1; 1Keio University, Yamagata, Japan; 2University of Manitoba, Winnipeg, MB; 3Kyoto Institute of Technology, Kyoto, Japan

ThP 126 Characterisation of a Bacterial Proteome by Means of Ion Mobility Mass Spectrometry-Based Proteomics; Konstantinos Thalassinos1; Nisha Patel1;Susan E. Slade 1; Jim Langridge2; Chris Hughes 2; Scott Geromanos2; Marc V. Gorenstein2; Dan Golick3;Andrew Crombie1; J. Colin Murrell1; James Scrivens1;1University of Warwick, Coventry, UK; 2Waters Corporation, Manchester, UK; 3DanGo Designs Inc, Newton, MA

ThP 127 Comparing Gel-Eluted Liquid Fraction Entrapment Electrophoresis to Gel-LC for the Analysis of Complex Proteomics Samples; Issa Isaac; Cindy Brown; Chris Dill; Charles E. Witkowski, II; Jeremy L. Norris; Protein Discovery, Inc., Knoxville, TN

ThP 128 A High Resolution, Highly Reproducible and Sensitive nano_LC Configuration that is Particularly Suitable for Large-Scale Label-Free Expression Profiling; Frank Engler1; Xiaotao Duan 1; Jun Li1; Hao Wang2; Jun Qu1; 1University at Buffalo, Amherst, NY; 2UB_Pharmaceutical Science, Buffalo, NY

ThP 129 Time-Course Reproducibility of Clinical Samples Using Retentate Chromatography MALDI-TOF Mass Spectrometry; Sabine Jourdain1; Enrique Dalmasso1; Amanda Bulman1; Fiona Plows1; Martin Schuerenberg2; Akos Czibere3; Sonja Hartwig4; Stefan Lehr4; 1Bio-Rad Laboratories, Inc., Hercules, CA; 2Bruker Daltonics, Bremen, Germany; 3Harvard Stem Cell Institute, Boston, MA; 4German Diabetes Center, Duesseldorf, Germany

ThP 130 Comparison and Combination of Electrospray and MALDI Analyses of Complex Protein Mixtures; Sean L. Seymour1; Ignat Shilov1; Aaron Booy2; Doug Simmons2; Stephen A Tate 2; Alpesh Patel1; Christie L Hunter1; 1AB SCIEX, Foster City, CA; 2AB SCIEX., Concord, ON, Canada

PROTEOMICS: CLINICAL APPLICATIONS, 131 - 165 ThP 131 Comparative Proteome Analysis of Mycobacterium

Tuberculosis Using Label-Free Quantitative Method: the Response of Drug-Resistant and Drug-Sensitive Strains; Moo-Jin Suh 1; Alifiya S. Motiwala2; Hamid Alami1; Shih-Ting Huang1; Robert D. Fleischmann1;Scott N. Peterson1; David Alland2; Rembert Pieper1; 1J. Craig Venter Institute, Rockville, MD; 2University of Medicine and Dentistry of New Jersey, Newark, NJ


ThP 132 Multiplexed MRM Analysis and Absolute Quantitation of 25 Proteins Implicated in Coronary Artery Disease; Juncong Yang2; Michael Kuzyk2;Gabriela Cohen Freue3, 4; John Hill3; Bruce McManus3, 4;Christoph H. Borchers1; 1University of Victoria, Victoria, BC, Canada; 2UVic Genome BC Proteomics Centre, Victoria, BC, Canada; 3University of British Columbia, Vancouver, BC, Canada; 4PROOF Centre of Excellence, Vancouver, BC, Canada

ThP 133 Quantitative Proteomic Analysis of Human Monocyte-Derived Dendritic Cells after Exposure to Live Brugia malayi microfilariae; Zhaojing Meng1;Roshanak T. Semnani2; Thomas B. Nutman2; Timothy D. Veenstra1; 1SAIC-Frederick, Inc., Frederick, MD; 2NIAID, NIH, Bethesda, MD

ThP 134 Proteomics Study of the Interaction of Dimebon with Different Brain Regions in the Treatment of Alzheimer’s Disease; Milica Tesic Mark; Amy Frances Friss; Rong Wang; Mount Sinai School of Medicine, New York, NY

ThP 135 Quantitative Tracking of Binding and Cellular Uptake of Human Serum Transferrin in HeLa Cells;Shunhai Wang; Cedric Bobst; Igor A. Kaltashov; University of Massachusetts, Amherst, MA

ThP 136 Quantitation of EGFR and phosphoEGFR in FFPE Tissue; Sheeno Thyparambil1; Jenny Heidbrink Thompson 1; Jon Burrows1; David Krizman1; Marlene M. Darfler1; Paul Taylor2; Jiefei Tong2; Warren Shih3;Ming Tsao3; Michael Moran2; Todd Hembrough1;

1Expression Pathology Incorporated, Rockville, MD; 2Hospital for Sick Children, Toronto, ON; 3PrincessMargaret Hospital, Toronto, Canada

ThP 137 The Temporal Proteome Response to Severe Traumatic Injury in Human Leukocytes Revealed by Large-Scale Quantitative Proteomics Employing an 18O-Labeled Reference; Weijun Qian1; Carrie D Nicora1; Brianne O Petritis1; Athena Schepmoes1; Ron Moore 1; Matthew Monroe 1; Lyle Moldawer2; Ronald Maier3; Ronald Tompkins4; David Camp1; Richard D. Smith1; 1Pacific Northwest National Lab, Richland, WA; 2University of Florida, Gainesville, FL; 3University of Washington, Seatttle, WA; 4Massachusetts General Hospital, Boston, MA

ThP 138 Development of a Multiplexed Immuno MALDI (iMALDI) Mass Spectrometry Assay for Diagnosis of Hypertension Related Diseases; Jennifer D. Reid1, 3; D. Randal Mason3; Daniel T. Holmes2; Christoph H. Borchers1, 3; 1UVic Genome BC Proteomics Centre, Victoria, Canada; 2St. Paul's Hospital, Vancouver, BC, Canada; 3University of Victoria, Victoria, BC, Canada

ThP 139 A Targeted MS diagnostic for the multiplexed detection of liver enzymes in plasma for the detection of liver disease; Julie A Weisz; Suzanne Y Ngo; Christine C Wu; University of Colorado School of Medicine, Aurora, CO

ThP 140 Using Tandem MS to Elucidate the Metabolism of CNS-derived Apolipoprotein E3 and Apolipoprotein E4 in Young Normal Control Participants; Kristin Wildsmith; Bruce Patterson; Randall Bateman; Washington University, St. Louis, MO

ThP 141 Quantitative Analysis of Oxidative Modifications in HDL Using MRM; Hussein N Yassine2, 2; Mike Kimzey 1; Michael A Galligan1; Craig S Stump2, 3; George Tsaprailis1; Serrine S Lau1; 1Southwest Environmental Health Sciences Center, Tucson, AZ; 2Department of

Medicine, University of Arizona, Tucson, AZ; 3Southern Arizona VA Healthcare System, Tucson, AZ, Tucson, AZ

ThP 142 NCI’s Clinical Proteomic Technologies for Cancer - Building a Robust and Reliable Cancer Protein Biomarker Development Pipeline; Amir Rahbar1;Emily S. Boja1; Tara Hiltke1; Chris Kinsinger1; Mehdi Mesri1; Robert Rivers1; Henry Rodriguez1, 2; 1NCI/NIH, Bethesda, MD; 2Clinical Proteomic Technologies for Cancer Network, Bethesda, MD

ThP 143 Towards Early Detection of Breast Cancer Using Serum Peptide Profiling; Yuri E.M. Van Der Burgt;Berit Velstra; Wilma E. Mesker; Magnus Palmblad; Marco R. Bladergroen; Bart Mertens; Rob A.E.M. Tollenaar; André M. Deelder; Leiden University Medical Center, Leiden, Netherlands

ThP 144 Proteomic and Transcriptomic Analysis of Urinary Exosomes for Biomarker Discovery; David L. Hachey1; Zhen Wang1; Salisha Hill1; J. Matt Luther1;Peter E. Clark1; Shawn E. Levy2; Kevin L. Schey 1;1Vanderbilt University, Nashville, TN; 2HudsonAlphaInstitute for Biotechnology, Huntsville, AL

ThP 145 Application of Laser Capture Microdissection Proteomics for Biomarker Discovery in Chronic Wound Keratinocytes; Michael A. Freitas; John P. Shapiro; Sabyasachi Biswas; Sashwati Roy; Chandan K. Sen; Ohio State University, Columbus, OH

ThP 146 Sensitive, Reproducible Protein Identification from Laser Capture Microdissected Tissue; John Shapiro;Sabyasachi Biswas; Gerard Lozanski; Metin N. Gurcan; Sashwati Roy; Chandan K. Sen; Michael A. Freitas; Ohio State University, Columbus, OH

ThP 147 Novel Biomarkers of Platinum Resistance Identified by Proteomic-Based Analysis of Platinum Sensitive and Resistant Ovarian Cancer Cell Lines; Bunja Rungruang; Brian L. Hood; Mai Sun; Pang-Ning Teng; Nicholas Bateman; Melanie Flint; Thomas C. Krivak; Thomas P. Conrads; University of Pittsburgh Cancer Institute, Pittsburgh, PA

ThP 148 Comprehensive Analysis of Gold Nanoparticle-Serum Interactions; Nawei Zhang2; Yong Liang3; Joe Fernandez1; Chun Chen1; Haiteng Deng1; 1TheRockefeller University, New York, NY; 2BeijingChaoyang Hospital affiliated CMSU, Beijing, China; 3Taizhou Medical College, Taizhou, China

ThP 149 Proteomic Studies of Tracheal Aspirates Derived from Neonates with Respiratory Distress Syndrome Using GELFrEE Fractionation Coupled to nanoLC Mass Spectrometry; Michael Bereman 1; Daniela Tomazela1; Hillary Heins2; Sessions Cole2; Aaron Hamvas2; Michael J. Maccoss1; 1University of Washington, Seattle, WA; 2Washinton University, St. Louis, MO

ThP 150 Functional Characterization of Ubiquitination Profile Changes Induced by Oncogenic Protein NPM-ALK in ALK+ Anaplastic Large Cell Lymphoma Cells;Fang Wu 1; Peng Wang2; Raymond Lai2; Liang Li1;1Department of Chemistry, Edmonton, Canada; 2Department of Laboratory Medicine and Pathology, Edmonton, Canada

ThP 151 Proteomic Cell Surface Phenotyping of Differentiating Acute Myeloid Leukemia Cells;Andreas Hofmann1; Bertran Gerrits2; Alexander Schmidt1; Thomas Bock1; Damaris Bausch-Fluck1;Ruedi Aebersold1; Bernd Wollscheid1; 1Institute of Molecular Systems Biology, ETH Zurich, Zurich,


Switzerland; 2Functional Genomics Center Zurich, UZH/ ETH Zurich, Zurich, Switzerland

ThP 152 Progress In Standardizing Mass Spectrometry-based Platforms via Inter-laboratory Studies From Discovery to Verification (NCI-CPTAC Network Studies); Emily S. Boja1; Amir Rahbar1; Christopher Kinsinger1; Mehdi Mesri1; Tara Hiltke1; Robert Rivers1;Henry Rodriguez1, 2; 1NCI/NIH, Bethesda, MD; 2Clinical Proteomic Technologies for Cancer Network, Bethesda, MD

ThP 153 Searching Multiple Tyrosine Kinase Signaling Pathways Aberrantly Activated in Malignant Mesothelioma by Using Mass Spectrometry; YibaiChen; Craig Menges; Anthony Yeung; Joseph Testa; Fox Chase Cancer Center, Philadelphia, PA

ThP 154 Correlation of Cyclopamine with Proteome Response in Glioma Mouse Xenografts by MALDI Imaging Mass Spectrometry; Sara L. Frappier1; Anuraag Sarangi1; Michael Cooper1; Richard M. Caprioli2;1Vanderbilt University, Nashville, TN; 2Vanderbilt Univ Sch of Med, Nashville, TN

ThP 155 Characterization of Differentially Expressed Plasma Membrane Proteins Induced by NPM-ALK: Investigate Aberrant Signaling Pathways in ALK+ Anaplastic Large Cell Lymphoma; Difei Sun; Fang Wu ; Peng Wang; Raymond Lai; Liang Li; University of Alberta, Edmonton, AB

ThP 156 The Fat Depot-Specific Secretome of Human Fat Cell Progenitors; Yi Zhu; Tamara Tchkonia; Nino Giorgadze; Kuo-ching Liang; Peter W. Li; Carrie J. Holtz Heppelmann; H. Robert Bergen, III; James L. Kirkland; Mayo Clinic, Rochester, MN

ThP 157 Analysis of Membrane Proteins from the Prefrontal Cortex in Schizophrenia and Control Subjects; Bruno Manadas1; Jane English2; David R Cotter3; Mike J. Dunn2; 1Center for Neuroscience and Cell Biology, Coimbra, Portugal; 2University College Dublin, Dublin, Ireland; 3Royal College of Surgeons, Dublin, Ireland

ThP 158 Detection of Alpha-Enolase and Triosephosphate Isomerase in Sera from Patients with Pancreatic Cancer by Autoantibodiomics Analysis; Toyofumi Nakanishi1; Yoshiharu Miyamoto1; Nobuhiko Tanigawa1; Takayuki Takubo1; Kyoichi Takaori2;1Osaka Medical College, Osaka, Japan; 2Kyoto University Graduate School of Medicine, Kyoto, Japan

ThP 159 Effect of mTOR-Inhibitor Rapamycin on U87MG Glioblastoma Cell Line Proteome; Marcela Gimenez1;Anelisa Ramao1; Helen Julie Laure1; Clarice Izumi1;Sueli Mieko Oba Shinjo2; Suely Kazue Nagahashi Marie2; Jose Cesar Rosa1; 1University of Sao Paulo, Ribeirao Preto, Sao Paulo - Brazil; 2Medical School of Sao Paulo, Sao Paulo, Sao Paulo - Brazil

ThP 160 Shotgun Proteomics Analysis of the Osteoblasts Differentiation of Rat Bone Marrow Stem Cells;Leonardo Barcelos de Paula1; Fabíola Singaretti de Oliveira2; Marcela Gimenez1; Lucas Oliveira Sousa1;Helen Julie Laure1; Adalberto Luiz Rosa2; Jose Cesar Rosa1; 1University of Sao Paulo - USP, Ribeirao Preto, Sao Paulo - Brazil; 2Dentistry School of Ribeirao Preto-USP, Ribeirao Preto, Sao Paulo - Brazil

ThP 161 Analysis of Posttranslational Modifications of Human Free Light Chains by Means of Mass Spectrometry;Petra Kapková; University of Würzburg, Würzburg, Germany

ThP 162 Effect of Glyco-oxidative Modifications on Plasma Fibrinogen Function in Diabetes; Hussein N. Yassine1;

Michael Kimzey 2; Michael A Galligan2; Craig S. Stump1, 3; George Tsaprailis2; Serrine S Lau2;1Department of Medicine, University of Arizona, Tucson, AZ; 2Southwest Environmental Health Sciences Center, University of Arizona, Tucson, AZ; 3Southern Arizona VA Healthcare System, Tucson, AZ

ThP 163 Profiling of Oxidative Stress Proteomic Signature in the Plasma of Type II Diabetic Zucker Rats; Ashraf G. Madian 1; Angela Myracle2; Naomi Diaz-Maldonado1; Elsa M. Janle2; Fred Regnier1; 1Departmentof Chemistry, Purdue University, West Lafayette, IN; 2Department of Foods and Nutrition, Purdue University,West Lafayette, IN

ThP 164 Identification of Novel P53-Interacting Candidates;Steve Nguyen; Sofia Macieira; Matthias Mann; Max Planck Institute, Martinsried, Germany

ThP 165 Clinical Use of Intact Globin Chain Mass Spectrometry in the Identification of Hemoglobin Variants; Patricia Wendt; Frank W. Crow; Ryan Morse; Jennifer Oliveira; James Hoyer; Mayo Clinic, Rochester, MN

QUANTITATIVE PROTEOMICS IV, 166 - 189 ThP 166 Comparison of Protein Quantification by Selective

Reaction Monitoring MS and ELISA/LUMINEX Techniques; Wenbo Zhi; Jin-Xiong She; Meiyao Wang; Medical College of Ga, Augusta, GA

ThP 167 A Targeted Proteomics Approach to Quantitate the Levels of Delta 6-desaturase in Developing Safflower Seeds; Rudy Alvarado1; Isabel Dicely2; Ken Mai2; John Goodstal2; Daniel Facciotti2; Brett Phinney1; 1UC Davis Proteomics Core Facility, Davis, CA; 2Arcadia Biosciences, Davis, California

ThP 168 Monitoring Post-Translational Modifications in Human Gamma S Crystallin by Multiple Reaction Monitoring (MRM) Mass Spectrometry; Debra McMillen; Phillip Wilmarth; Larry David; BMB, OHSU, Portland, OR

ThP 169 An nLC/MS/MRM Based Standard Addition Method for Absolute Quantitation of FcRn Protein Copy Numbers in Cell Lysates; Amy Lu; Fei Hua; Betty Tang; Yan Zhang; Albert B Seymour; Bruce Gomes; Ru Wei; Pfizer RTC, Cambridge, MA

ThP 170 Quantification of Poly(ADP-ribose)-Binding Proteins Using Multiple Reaction Monitoring (MRM); Michel Boutin1; Sylvie Bourassa1; Jean-Philippe Gagné2;Maxim Isabelle2; Guy G. Poirier1, 2; 1Proteomics Platform, Laval University, CHUQ, Québec, Canada; 2Laval University, Medical Research Center, CHUQ, Québec, Canada

ThP 171 MRM-Based Quantitation of SNAP-25 Protein Isoforms in Cells and Human Brain Samples; Vilte E Barakauskas1; Annie Moradian 4; Grace SW Cheng4;Andrew J Dwork3; William G Honer1; Gregg Morin2, 4;1University of British Columbia Dept. of Psychiatry, Vancouver, Canada; 2University of British Columbia Dept. Med. Genetics, Vancouver, Canada; 3New York State Psychiatric Institute, New York, New York; 4Genome Sciences Center, BC Cancer Agency, Vancouver, Canada

ThP 172 Predicting Response to Chemotherapy in Colorectal Cancer Using Selected Reaction Monitoring; Matthew McKay1, 2; Sarah Randall1; Dana Pascovici2; Wei Chua3;Stephen Clark3; Mark Molloy1, 2; 1Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; 2APAF, Macquarie


University, Sydney, Australia; 3Dept. of Medicine, Concord Repatriation Hospital, Sydney, Australia

ThP 173 Quantifying the Cellulosome Protein Machine in Whole-Cell Lysate from Clostridium thermocellum as a Function of Cellulosic Substrate; Andrew B. Dykstra 1, 2; Lois A. St. Brice3; Joel Kreps4; Kelsey D. Cook 2, 5;Lee R. Lynd3; Robert L. Hettich1; 1Oak Ridge National Laboratory, Oak Ridge, TN; 2University of Tennessee, Knoxville, TN; 3Dartmouth College, Hanover, NH; 4Verenium Corporation, San Diego, CA; 5National Science Foundation, Arlington,VA

ThP 174 Quantitative Analysis of STAT3 in Cardiac Tissue Using Multiple Reaction Monitoring; Angel Aponte1;Darci Phillips2; Guanghui Wang1; Robert Balaban2;Marjan Gucek1; 1Proteomics Core, NHLBI/NIH, Bethesda, MD; 2Laboratory of Cardiac Energetics, NHLBI/NIH, Bethesda, MD

ThP 175 Sensitive, Reproducible, Robust and High Speed MRM Assay for Absolute Quantitation of 75 Proteins in Human Plasma; Derek S. Smith1; Michael A. Kuzyk1; Tyra J. Cross1; Jennifer L. Proc1; Juncong Yang1; Dominik Domanski1; Christie L. Hunter2;Matthew M. Champion2; Christoph H. Borchers1,3; 1UVicGenome BC Proteomics Centre, Victoria, BC, Canada; 2AB Sciex, Foster City, CA; 3University of Victoria, Victoria, BC, Canada

ThP 176 Targeted Approach to the Identification of Theoretical Proteins from Mycoplasma Hyopneumoniae Strain 232; Sarah Kruger1; Matthew Padula2; Steven Djordjevic2; 1AB SCIEX, Keperra, Australia; 2UTS, Sydney, Australia

ThP 177 Rapid Assay Development and Refinement for Targeted Protein Quantitation Using an Intelligent SRM (iSRM) Workflow; Reiko Kiyonami; Amol Prakash; Vlad Zabrouskov; ThermoFisher Scientific, San Jose, CA

ThP 178 SRM-Based Quantitative Analysis of IGF-1R and IRS1 Directly in FFPE Tissues for Objective Pathological Analysis in Cancer Related Signaling Pathway; Amol Prakash1; Bryan Krastins1; David Sarracino 1; Michael Athanas2; Taha Rezai1; Todd Hembrough3; Jenny Hiedbrink-Thompson3; Sheeno Thyparambil3; Jon Burrows3; David Krizman3; Mary F. Lopez1; 1Thermo Fisher Scientific, Cambridge, MA; 2VAST Scientific, Cambridge, MA; 3ExpressionPathology Inc., Rockville, MD

ThP 179 Reducing Artifacts and Defining the Linear Quantitative Range of pSILAC; Isabelle Kelly;Leonard J Foster; Center for High-Throughput Biology, UBC, Vancouver, BC, Canada

ThP 180 SILAC and Label-Free Approaches for Quantitatively Identifying Caveolae Proteome; Yu Zi (Emma) Zheng1; Michelle M. Hill4; Tom Hennessy2;Robert G. Parton3; Leonard J. Foster1; 1Univeristy of British Columbia, Vancouver, Canada; 2Agilent Technologies, Singapore, Singapore; 3IMB, University of Queensland, Brisbane, Australia; 4Diamantina Institute, University of Queensland, Brisbane, Australia

ThP 181 iTRAQ Application & Pragmatism: Dependency on Time of MS/MS Triggering, Label Distribution, and Mass Resolution with Sample Complexity; Saw Yen Ow; Malinda Salim; Josselin Noirel; Caroline Evans; Phillip C Wright; University of Sheffield, Sheffield, UK

ThP 182 Differential Proteome Analysis of AKT1 and Mutant AKT1(E17K) in Human Mammary Epithelial Cells by Mass Spectrometry; Tony Tegeler; Bodour Salhia;

Matthew Rosenow; Jian Liu; Linda Nagore; Ashoka Polpitiya; John Carpten; Konstantinos Petritis; Translational Genomics Research Institute, Phoenix, AZ

ThP 183 Expression Protein Profile of Exosomes of Hepatic Cell Lines; Eva Rodriguez-Suarez1; Javier Conde-Vancells3; E. Gonzalez2; S.C. Lu4; Rune Matthiesen2;Felix Elortza1; Jose María Mato3; Juan M Falcón Pérez3;1Proteomic Platform, CIC bioGUNE, ProteoRed, Derio, Spain; 2Institute of Molecular Pathology and Immunology, O Porto, Portugal; 3Metabolomic Unit, CIC bioGUNE, CIBERehd, Derio, Spain; 4Keck School of Medicine, Unv Southern California, Los Angeles, CA

ThP 184 Label-Free Proteomic Quantification Using an Ultra High Resolution (UHR) TOF and an Integrated Bioinformatics Platform; Adam Dowle1; Diana Saggese 1; Ed Bergström 1; David Ashford 1; Jerry Thomas1; Jane Thomas-Oates 1; Markus Lubeck2;Wolfgang Jabs2; Carsten Baessmann 2; 1Centre of Excellence in Mass Spectrometry, University of York, York, UK; 2Bruker Daltonik GmbH, Bremen, Germany

ThP 185 Mass Spectrometrical Analysis of Senescence-Associated Protein Changes in Cultured Human Fibroblasts Using a Label-Free Proteomics Approach; Daniel Waldera1; Ana-Maria Florea2;Sebastian Link1; Birgit Korte1; Barbara Sitek1; Helmut E. Meyer1; Guido Reifenberger2; Kai Stühler1;1Medizinisches Proteom-Center, Bochum, Germany; 2Institut für Neuropathologie, Duesseldorf, Germany

ThP 186 Comparison of Label-Free Protein Quantification Approaches for Chemical Proteomics; Zhixiang Wu;Kurt Fellenberg ; Simone Lemeer; Bernhard Kuster; Technical Universitiy Munich, Freising, Germany

ThP 187 Implementation of 18O Labelling for Urine Proteome Quantification Using Accurate Mass Tag Retention Time data Base; Eugene Nikolaev1; Ilya A Agron 2;Dmitry M. Avtonomov2; Igor Popov4; Alexey Kononikhin2; Maria I. Indeykina5; Oxana P. Trifonova7;Sergey Moshkovsky6; 1The Institute for Energy Problems of Chemical Phys, Moscow, Russian Federation;2INEPCP RAS, Moscow, Russian Federation; 4Russian Academy of Sci, Moscow, Russian Federation; 5Moscow State University, Moscow, Russian Federation; 6The Institute for Biomedical Chemistry Russian Aca, Moscow, Russia; 7Inst. Biomedical Problems, Moscow, Russian Federation

ThP 188 Rapid and Quantitative PAcIFIC Method for Data-Independent Proteome Profiling; Alexandre Panchaud; Sunhee Jung; Shawna M. Hengel; Jimmy K. Eng; Scott A. Shaffer; David R. Goodlett; University of Washington, Seattle, WA

ThP 189 Development of a Reference Measurement Procedure to Quantify Urinary Albumin; Ashley S. Beasley;David Bunk; Karen Phinney; National Institute of Standards and Technology, Gaithersburg, Maryland

PEPTIDES FRAGMENTATION AND SEQUENCING, 190 - 215

ThP 190 Effect of Acidic Amino Acid on b-type Sequence Scrambling; Talat Yalcin; Ahmet Emin Atik; IYTE, Urla-Izmir, Turkey

ThP 191 On the Existence of Structurally Different Isobaric Bn Fragment Ions; Touradj Solouki; Behrooz Zekavat; Mahsan Miladi; University of Maine, Orono, ME

ThP 192 Femtosecond Laser-induced Ionization/Dissociation (fs-LID) Tandem Mass Spectrometry for the Enhanced Characterization of Low Charge State Protonated Phosphopeptide Ions; Scott A. Smith1;


Christine L. Kalcic1; Kyle A. Safran1; Paul M. Stemmer2; Marcos Dantus1; Gavin E. Reid 1; 1MichiganState University, East Lansing, MI; 2Wayne State University, Detroit, MI

ThP 193 Small Neutral Losses in the Electron Capture Dissociation and Electron Transfer Dissociation of Nitrotyrosine-Containing Peptides; Andrew Jones;Helen Cooper; University of Birmingham, Birmingham, UK

ThP 194 A Sweet Tooth that Bites: Site Selective Radical Induced Fragmentation at O-Glycosylated Residues;Jolene K. Diedrich; Ryan R. Julian; University of California, Riverside, Riverside, CA

ThP 195 Contribution of ECD and ETD Activation Modes in the Top-Down Identification of Peptidic Toxins Containing Disulfide Bonds; Chafia Bennaceur1, 2;Carlos Afonso1; Sandra Alves1; Anne Bossee2; Jean-Claude Tabet1; 1Université Paris 6/UMR7201, Paris, France; 2DGA CBRN Defence, Vert-Le-Petit, France

ThP 196 Effect of Cysteic Acid Position on the Negative Ion Fragmentation of Proteolytic Derived Peptides; Brad J. Williams; Kevin L. Kmiec; William K. Russell; David H. Russell; Texas A&M University, College Station, TX

ThP 197 Radical-Induced Dissociation of N-Nitrosopeptides Through Collisional Activation; Eric R. Knudsen;Ryan R. Julian; University of California, Riverside, Riverside, CA

ThP 198 Exploring the Potential of Electron Transfer Dissociation Mass Spectrometry for the Analysis of PNA-containing Branched Peptides; Luc Tessier1;Jenny Phipps2; Wei He2; Russ Blacher2; Raphael Terreux2; John Kelly1; 1National Research Council of Canada - Institute of, Ottawa, Canada; 2PharmaGapInc., Ottawa, Canada

ThP 199 A Study on the Fragmentation Characteristics of Large-size Bioactive Peptides under Electron-transfer Dissociation Conditions; Chhabil Dass; The University of Memphis, Memphis, TN

ThP 200 Exploring Cyclic Peptide Fragmentation by Electron Transfer Dissociation Coupled to Orbitrap Mass Spectrometry; Hao Wang1, 2; Xiaotao Duan 1, 2; Jun Qu1,

2; 1University at Buffalo, Amherst, NY; 2CoE in Bioinformatics & Life Sciences, Buffalo, NY

ThP 201 Controlling the Fragmentation Behavior of Tryptic and Lys-N Peptides upon Electron Transfer Induced Dissociation by Chemical Modification; Marco Hennrich; Paul J. Boersema; Albert J.R. Heck ; Shabaz Mohammed; Utrecht University, Utrecht, Netherlands

ThP 202 Probing the Fragmentation Behavior of Transition Metal-Peptide Complexes; Heather Malone Watson;Carolyn J. Cassady; University of Alabama, Tuscaloosa, AL

ThP 203 Metal Ion Interaction with Cyclodepsipeptides: Tandem Mass Spectrometry and NMR Study of Isariins and Isaridins; Raja Banerjee1, 2; S Sudarslal2;R. S. Ranganayaki2; S. Raghothama3; 1West Bengal University of Technology, Kolkata, India; 2Molecular Biophysics Unit, IISC, Bangalore, India; 3NMRResearch Centre, Indian Institute of Science, Bangalore, India; 4NMR Research Centre, Indian Institute of Science,, Bangalore, India

ThP 204 Comparing the Formation and Gas Phase Fragmentation, CID, IRMPD, ECD, of Peptide Radical Cations from [Cu(II)(tpy)(M+H)]3+• and [M+Cu]2+ Complexes; Anastasia Kalli; Sonja Hess; CalTech, Pasadena, CA

ThP 205 Structural Parameters for Suppression and Re-Activation of the N-C Cleavages and H. Loss from [M+nH] (n-1)+ ; Pui Shuen Wong; Tak-Wah Dominic Chan; The Chinese Univ. of Hong Kong, Hong Kong Sar, China

ThP 206 Investigation of Fragmentation Mechanism of Peptides Containing Histidine Residue; Talat Yalcin; Cagdas Tasoglu; IYTE, Urla-Izmir, Turkey

ThP 207 Enhanced Data-Independent Acquisition Based LC-MS/MS Through Spectrum Deconvolution and Instrument Method Optimization; Jarrett Egertson1;Jesse D. Canterbury1; Gennifer Merrihew1; Gregory Finney2; Marshall W. Bern3; Michael J. Maccoss1;1University of Washington, Seattle, WA; 2Univ of Washington, Genome S, Seattle, WA; 3Palo Alto Research Center, Palo Alto, CA

ThP 208 Optimization of Data-Dependent LC-MS/MS Using parallel CID/ETD Ion Trap Systems; Hans Dalebout;Ekaterina Mostovenko; Yuri E.M. Van Der Burgt; André M Deelder; Magnus Palmblad; Leiden University Medical Center, Leiden, Netherlands

ThP 209 Characteristics of MS/MS Fragmentation Pattern in Endogenous Non-Tryptic Peptides; Hernando Escobar2; David K. Crockett2; Eduardo Reyes-Vargas1;Peter Jensen1, 2; Julio C Delgado1, 2; 1University of Utah. Department of Pathology, Salt Lake City, Utah; 2ARUPLaboratories, Salt Lake City, Utah

ThP 210 Optimizing Linear Ion Trap Data Dependent Parameters for Maximum Coverage in Proteomics Survey Experiments; Julie Horner1; Roger G. Biringer1;August Specht 2; Andreas F Huhmer1; 1Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher, San Jose, CA

ThP 211 Sequence Confirmation of Cyclic Thioether Bridged Peptides by Nanospray CID MS/MS; Denise Keen;Roger Moore; Gabriel Gugiu; City of Hope, Duarte, CA

ThP 212 Peptide Fragmentation at Atmospheric Pressure;Vadym Berkout; Vladimir M. Doroshenko; MassTech, Inc., Columbia, MD

ThP 213 Using a Fragmentation Database to Derive VUV Photodissociation Selection rules and Interpret Peptide Spectra; Xiaohui Liu; James P. Reilly; Indiana University, Bloomington, IN

ThP 214 Comparison of IR-MPD Spectra of Peptide Fragments to Computations: Conformational Searching and Diagnostic Vibrations; Long Yu1, 1;Nicolas Polfer1, 2; 1, Gainesville, FL; 2University of Florida, Gainesville, FL

ThP 215 The Effect of Peptide Size on the Occurrence of Hydrogen Scrambling upon Collisional Activation in Hydrogen/Deuterium Exchange Mass Spectrometry;Sabine Amon; Ole N. Jensen; Thomas J.D. Jorgensen; University of Southern Denmark, Odense, DenmarkBIOMARKER DISCOVERY III, 216 - 242

ThP 216 Electron Transfer Dissociation Investigations of the Urinary Proteome and Peptidome in Pregnancy;Sarah R Hart; Keele University, Newcastle-Under-Lyme, UK

ThP 217 Metabolic Profiling in Urine Samples from Patients with Invasive Histoplasma Infection; Mark M. Kushnir1; David K. Crockett1; Alan L. Rockwood1, 2;Joann L. Cloud1; Edward R. Ashwood1, 2; 1ARUP Institute, Salt Lake City, UT; 2Department of Pathology, University of Utah, Salt Lake City, UT

ThP 218 Proteomic Biomarker Discovery for Diabetic Complications: Urinary Protein Profiles in CACTI Study; Daniela M Schlatzer 1; Jean-Eudes Dazard1; Rob


Ewing1; Giridharan Gokulrangan 1; Janet Snell-Bergeon2; David Maahs2; Marian Rewers2; Mark R. Chance1; 1Case Western Reserve University, Cleveland,OH; 2Barbara Davis Center for Childhood Diabetes, UCD, Denver, Colorado

ThP 219 Comprehensive and Quantitative N-Glycoproteome Analysis Method Reveals Novel Accessible Biomarkers in human Breast Cancer; Andrei Turtoi1;Yannick Greffe1; Edwin De Pauw2; Vincent Castronovo1; 1University of Liege, Liege, Belgium; 2Liege University, Liege, Belgium

ThP 220 Discovery of Urinary Glycoprotein Biomarkers in Bladder Cancer; Na Yang 1; Shun Feng 1; Kerby Shedden1; Xiaolei Xie 1; Yashu Liu 1; Xiang Fan2;Steven Goodison3; Charles Rosser3; David M. Lubman 1;1University of Michigan, Ann Arbor, MI; 2Shimazdu Biotech, Pleasanton, CA; 3M. D. Anderson Cancer Center, Orlando, FL

ThP 221 A Comparative Glycoproteomics Approach to the Discovery of Biomarkers in Prion Diseases; Lingjun Li1; Xin Wei1; Allen Herbst2; Di Ma1; Judd Aiken2;1University of Wisconsin, Madison, WI; 2University of Alberta, Edmonton, Canada

ThP 222 A Lectin Affinity-Based Biomarker Discovery Strategy Targeting Cancer-specific Glycoproteins in Human Plasma; Birgit Schilling1; Penelope M. Drake2;Richard K. Niles2; Miles Braten2; Dylan J. Sorensen1;Eric Johansen2; Jason M. Held1; Demetris Iacovides3;Steven C. Hall2; H. Ewa Witkowska2; Joe W. Gray3;Bradford W. Gibson1; Susan J. Fisher2; 1Buck Institute for Age Research, Novato, CA; 2University of California San Francisco, San Francisco, CA; 3Lawrence Berkeley National Laboratory, Berkeley, CA

ThP 223 Quantitative Glycoproteomics of Ovarian Cancer Serum Using Lectin Enrichment Methodology; Punit Shah; Julie Hafner; Zacharie Nickens; Vivekananda Shetty; Ramila Philip; Immunotope, Inc., Doylestown, PA

ThP 224 Study of N-Linked Glycopeptide Serum Biomarkers in Patients with Thyroid Related Disorders and Breast Cancer by LC-ESI-MS/MS; Alejandro Cohen;Rita Kostyleva; Ken Chisholm; Dev Pinto; IMB National Research Council of Canada, Halifax, Canada

ThP 225 Identification and Confirmation of Biomarkers for Hepatocellular Carcinoma Using Quantitative Analysis of Glycoproteins and Their Glycosylations;David M. Lubman; Yashu Liu ; Chen Li; Jintang He ; Jorge Marrero; University of Michigan, Ann Arbor, MI

ThP 226 Identification of Cell Surface Glycoprotein Markers for Glioblastoma-Derived Stem-Like Cells; Jintang He 1; Yashu Liu 1; Xiaolei Xie 1; Mary Soules1; Xing Fan1;Fan Xiang2; David M. Lubman 1; 1University of Michigan, Ann Arbor, MI; 2Shimadzu Biotech, Pleasanton, CA

ThP 227 A Two-Pass, Informatics-Driven, Label-Free Discovery Workflow for Discovery of Neurovascular Mediators in PFO Related Stroke; Michael Athanas1;David Sarracino 2; Taha Rezai2; Amol Prakash2; Jennifer Sutton2; Bryan Krastins2; Mingming Ning3; Mary F Lopez2; 1VAST Scientific, Cambridge, MA; 2Thermo Fisher Scientific, Cambridge, MA; 3Mass General Hospital/Harvard Medical School, Boston, MA

ThP 228 Comparative LC-MS Analysis of Thin Fresh Frozen Myocardial Tissue Sections from Transgenic Mice with H-Ras v12 Induced Cardiomyopathy; Xiaoying Ye1; Bih-Rong Wei2; Heather R. Shive2; Donald

Johann3; Mia R. Kumar2; R. Mark Simpson2; Timothy D. Veenstra1; Josip Blonder 1; 1SAIC-Frederick, Inc., Frederick, MD; 2National Cancer Institute, Bethesda, Maryland; 3NIH, Bethesda, MD

ThP 229 Label-Free MS Identification of Biomarkers of Metabolic Disorder Associated Cardiovascular Disease in a Mouse Model; Mark E. Mccomb1; David H. Perlman1; Deborah A. Siwik1; Vivek N. Bhatia1;Wilson Colucci2; Richard A. Cohen1; Catherine E. Costello1; 1Boston University School of Medicine, Boston, MA; 2Boston Medical Center, Boston, MA

ThP 230 iTRAQ Analysis Demonstrates that the Protein Signature of Aged Rat Ventricle is Unique from That of a Pressure Overload Model; Jennifer Grant1; Amy Bradshaw2; Catalin Baicu2; Susana Comte-Walters3;John Schwacke4; Michael Zile2; Kevin Schey5; 1BiologyDept., University of Wisconsin-Stout, Menomonie, WI; 2Cardiac Division, Dept. of Medicine, MUSC, Charleston, SC; 3Dept. of Cell and Mol. Pharmacology, MUSC, Charleston, SC; 4Biochemistry and Molecular Biology, MUSC, Chareston, SC; 5Mass Spectrometry Research Center, Vanderbilt Univ, Nashville, TN

ThP 231 Extensive Oxidative Protein Modifications Observed in Human Plasma and Candidate Biomarkers of Systemic Chronic Inflammatory and Oxidative Stress; Xu Zhang; Marina Gritsenko; Matthew Monroe ; Ron Moore ; David Camp; Diana Bigelow; Weijun Qian; Jon Jacobs; Joel Pounds; Richard D. Smith; Pacific Northwest National Lab, Richland, WA

ThP 232 Endogenous Peptide and Protein Release with Early Phase Ischemia / Reperfusion Injury; Melanie White;Lia Moshkanbaryans; Alistair Edwards; Benjamin Parker; Brett Hambly; Stuart Cordwell; The University of Sydney, Sydney, Australia

ThP 233 A study on the Development of Myocarditis Biomarker Discovery Technology Using Proteome/Metabolome Research of Animal Rat Models; Jong Bok Seo; Joo Hee Chung; Soo Young Kim; Eunjung Bang; Sang Goo Kim; Kwan Soo Hong; Korea Basic Science Institute, Seoul, South Korea

ThP 234 Quantitative Comparative Proteomic Analysis of Cytoplasmic Lipid Droplets Associated With Alcoholic Liver Disease; Brittany D.M. Hodges 1; Hide Tsukamoto2; Christine C. Wu 1; 1University of Colorado School of Medicine, Aurora, CO; 2University of Southern California, Los Angeles, CA

ThP 235 Identification of Protein Interaction Networks in Alzheimer’s Disease Using Quantitative Proteomics;Cristina Osorio; Robert DeKroon; Jennifer Robinette; Sergio Mejia; Carol Parker; Eric Hamlett; Oscar Alzate;University of North Carolina, Chapel Hill, NC

ThP 236 Quantitation of Isomerized Forms of Native Alzheimer’s Amyloid-Beta Peptide Using MS/MS Fragmentation; Maria Indeykina3; Igor Popov3; Alexey Kononikhin1; Nina Khristenko4; Eugene Nikolaev1;Sergey Kozin2; 1The Institute for Energy Problems of Chemical Phys, Moscow, Russian Federation; 2Institute of Biomedical Chemistry RAMS, Moscow, Russian Federation; 3Institute of Biochemical Physics RAS, Moscow, Russian Federation;4Moscow Institute of Physics and Technology, Moscow, Russian Federation

ThP 237 An Improved 2D-DIGE Analysis to Simultaneously Detect Changes in Protein Expression and Oxidative Modification; Robert Dekroon1, 2; Sun Yong Jeong 1, 2;Eric Hamlett2; Cristina Osorio1; Jennifer Robinette1;Mihaela Mocanu1; Oscar Alzate1, 2; 1UNC-Systems


Proteomics Center, Chapel Hill, NC; 2Department of Cell and Developmental Biology, Chapel Hill, NC

ThP 238 Biomarker Discovery in Alzheimer’s Disease by Proteomic Approaches; Ming-Hui Yang2; Hung Su1;Hsin-Chieh Wu1; Li-Jhen Chen1; Jentaie Shiea 2; Yu-Chang Tyan1; 1Kaohsiung Medical University, Kaohsiung, Taiwan; 2National Sun Yat-Sen Univ., Kaohsiung, Taiwan

ThP 239 Vitamin D Binding Protein (Gc) as a Urinary Biomarker of Subclinical Kidney Damage; Rhonda L. Pitsch; Pavel Shiyanov; Mitchell Meade; Frey Jeanette; Camilla Mauzy; John Schlager; 711th Human Performance Wing, WPAFB, OH

ThP 240 Label-free Proteomic Profiling of D-Serine-induced Toxicity Biomarkers in Rat Urine; Lining Qi; Pavel Shiyanov; John Schlager; US Air Force Research Lab, Wright-Patterson Air Force Base, OH

ThP 241 Identification of Human Tear Fluid Biomarkers in Vernal Keratoconjunctivitis Syndrome Using iTRAQ Quantitative Proteomics; Angelo Palmigiano1; Elisa Mazzola1; Angela Messina1; Serena Milazzo1; Andrea Leonardi2; Domenico Garozzo1; 1CNR-ICTMP, Catania, ITALY; 2University of Padova, Neuroscience, Padova, Italy

ThP 242 Leveraging Parallel Mass Spectrometry Platforms to Identify Biomarker Signatures of Oropharyngeal Candidiasis in Patient Saliva; Sarah Schwartz1; James Carlson1; Gary Ross2; Sandra Altarawneh3; Steven Offenbacher3; Sompop Bencharit3; 1DHMRI, Kannapolis, NC; 2Bio-Rad Laboratories, Hercules, CA; 3UNC-Chapel Hill, Chapel Hill, NC

PEPTIDES PTM: PHOSPHORYLATION & GLYCOSYLATION, 243 - 263

ThP 243 Eeukaryotic-Like Protein Kinase (Stk)-Mediated Protein Phosphorylation in Escherichia coli; Hye Kyong Kweon; David Friedman; Philip Andrews; The University of Michigan, Ann Arbor, MI

ThP 244 Enrichment and Analysis of Phosphorylation on a Planar MALDI Target; Petri Kouvonen; Eeva Rainio; Veronika Suni; Paivi Koskinen; Garry Corthals; Turku Centre for Biotechnology, Turku, Finland

ThP 245 MS Characterization of Phosphorylations of the Homer2 Scaffolding Protein in Brain; Rob Helton1;Santiago E. Farias1; Matthias Klugmann2; Karen K. Szumlinski3; Christine C. Wu 1; 1University of Colorado School of Medicine, Aurora, CO; 2University of Mainz, Mainz, Germany; 3University of Santa Barbara, Santa Barbara, CA

ThP 246 The FLEXI-Kinase in vitro Assay for the Identification of Kinase-Specificities in a Phosphorylation Signaling Cascade; Sasha Singh1, 2;Parizad Bilimoria2; Dominic Winter1, 2; Judith Steen1, 2;Azad Bonni2; Hanno Steen1, 2; 1Children's Hospital Boston, Boston, MA; 2Harvard Medical School, Boston, MA

ThP 247 Automated, High Confidence Phosphorylation Site Localization Using a Combination of CID and ETD MS/MS Spectra; Thomas A Hansen; Marc Sylvester; Ole N. Jensen; Univ. of Southern Denmark, Odense, Denmark

ThP 248 Highly Selective Enrichment of Phosphopeptides by Fe3+-IMAC Nanodiamonds for Direct MALDI MS analysis; Chia Wei Hung; Shi-Zhe Huang; Chih-Che Wu; National ChiNan University, Puli, Taiwan

ThP 249 Label-free Quantification of Co-eluting Isobaric Phosphopeptides of Insulin Receptor Substrate-1 by

HPLC-ESI-MS/MS; Paul Langlais; Lawrence J. Mandarino; Zhengping Yi; Arizona state university, Tempe, AZ

ThP 250 Proteomic Analysis OF Protein Phosphorylation in the Renal Response to Metabolic Acidosis; Dana Gammelgaard2; David Goldstrohm 1; Norman Curthoys2;Jessica Prenni1; 1Colorado State University, Fort Collins, CO; 2Colorado State Univ, Fort Collins, CO

ThP 251 Targeted Phosphorylation Site Analysis by MRM;Erik Haaf1; Ulrike Lanner1; Stephanie Lamer1; Anja Mast1; Sabrina Wendt2; Tilman Brummer1; Achim Kramer2; Andreas Schlosser1; 1Center for Biological Systems Analysis (ZBSA), Freiburg, Germany; 2Charité - Universitätsmedizin Berlin, Berlin, Germany

ThP 252 High Mass Accuracy Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Analysis of Human Phosphopeptides; Yuan Mao 1;Leonid Zamdborg2; Neil L. Kelleher2; Chris Hendrickson1; Alan G. Marshall1; 1National High Magnetic Field Laboratory, Tallahassee, FL; 2University of Illinois at Urbana-Champaign, Urbana, IL

ThP 253 Enrichment of Phosphorylated Peptides Using Arginine-Coated Oxide Compounds by MALDI-MS;Jinhee Kim; Hyo-Jik Yang; Seongjae Shin; Jeongkwon Kim; Chungnam National University, Daejeon, South Korea

ThP 254 Fixed Charge Derivatization for Enhanced Quantitative Collision Induced Dissociation (CID) and ‘Targeted’ Data-Dependant Electron Transfer Dissociation (ETD) Characterization of Phosphopeptides; Yali Lu1; Xiao Zhou1; Paul Stemmer2; Gavin E. Reid 1; 1Michigan State University, East Lansing, MI; 2Wayne State University, Detroit, MI

ThP 255 2 Dimensional Diagonal Capillary Electrophoresis - Mass Spectrometry for Phosphopeptide Analysis;Roza Wojcik; Oluvatosin Dada; Martin Sadilek; Norman Dovichi; University of Washington, Seattle, WA

ThP 256 Investigation of Gas-phase Phosphate Transfer of Phosphopeptides in Tandem Mass Spectrometry Using Collision-induced Dissociation and Electron Transfer Dissociation; Yuan Gao; Li-Rong Yu; Centerfor Proteomics, NCTR/FDA, Jefferson, AR

ThP 257 Comparative Assessment of Site Assignments in CID and ETD Spectra of Phosphopeptides Discloses Limited Relocation of Phosphate Groups; Nikolai Mischerikow; A.F. Maarten Altelaar; Shabaz Mohammed; Albert J.R. Heck; Utrecht University, Utrecht, Netherlands

ThP 258 Mapping Phosphorylated Histidines in Peptides by Mass Spectrometry Using Iodination Labeling;Qingyu Sun; Ryan Julian; UC, Riverside, Riverside, CA

ThP 259 Mapping ADP-Ribosylation Sites: Relative Abundance of ADP-Ribose Marker Ions and b and y Ions in CID Tandem Mass Spectra; Shawna Hengel;Laura Icenogle; Carleen Collins; David R. Goodlett; University of Washington, Seattle, WA

ThP 260 Method Development for Characterizing N-Linked Glycans and Glycopeptides by LC ESI FT-ICR Mass Spectrometry; Xu Wang1, 2; Mark R. Emmett2; Alan G. Marshall1, 2; 1Florida State University, Tallahassee, FL; 2Nat'l High Magnetic Field Lab, Tallahassee, FL

ThP 261 Structural Characterization of Glycopeptides: Integration of Complementary CID and ETD Based Information; Katalin F. Medzihradszky1, 2; Aenoch Lynn2; Robert Chalkley2; Peter R Baker2; Zsuzsanna


Darula1; A.L. Burlingame2; 1Biological Research Center, Szeged, Hungary; 2UCSF, San Francisco, CA

ThP 262 Rigorous Determination of Site-specific Glycosylation Heterogeneity by the Negative Ion Mode Analysis of Glycopeptides; Charles Nwosu1; John S. Strum1; Hyun Joo An1; Bruce German2; Carlito Lebrilla1; 1University of California Davis, Chemistry Departme, Davis, CA; 2Univ. of California Davis, Food Science & Tech., Davis, CA

ThP 263 Site Identification of O-Linked Glycans on Large Peptides by Electron Transfer Dissociation Mass Spectrometery; Danielle L Pace; John Valliere-Douglass; Theresa martinez; Gerd Kleemann; Amgen, Seattle, WA

IMMUNOLOGY, 264 - 281 ThP 264 The Promiscuous Role of -Defensins is by Mass

Spectrometry - Interrogation of Defense; Martin De Cecco1; Kirsty Tyrell3; Sam Capewell1; Hayden Eastwood2; Derek Macmillan4; Julia Dorin3; Dominic Campopinano1; Perdita Barran1; 1The University of Edinburgh, Edinburgh, UK; 2The Centre of Excellence in Modelling and Analysis, Stellenbosch, South Africa; 3MRC-Human Genetics Unit, Edinburgh, UK; 4UCL, London, UK

ThP 265 Epitope Structure of the Carbohydrate Recognition Domain of Asialoglycoprotein Receptor to a Monoclonal Antibody Revealed by Proteolytic Excision Mass Spectrometry; Michael Przybylski1;Raluca Stefanescu1; Rita Born2; Adrian Moise1; Beat Ernst2; 1University of Konstanz, Department of Chemistry, 78457 Konstanz, Germany; 2University of Basel, Pharmacenter, 4056 Basel, Switzerland

ThP 266 Understanding Genetic Factors of Vaccine Response and their Effect on Innate and Adaptive Immunity: Typhoid and Cholera a Case Study; James Stephenson1; Michael Gardner1; Megan Rowland1;Diane Wagener1; Paul Mosquin1; Herman Staats2;Shiying Wu1; Nikhil Garge 1; Dana Talley1; Carol Whisnant1; Jonathan L. Bundy4; Partha Majumder3;1Research Triangle Institute, Durham, NC; 2DukeUniversity Medical School, Durham, NC; 3Indian Statistical Institute, Calcutta, India; 4Centers For Disease Control, Atlanta, GA

ThP 267 Invariant Chain Regulates the Major Histocompatibility Complex Class II Peptide Repertoire of Cell-Based Cancer Vaccines; Olesya Chornoguz; Lydia Grmai; Alexei Gapeev; Suzanne Ostrand-Rosenberg; UMBC, Baltimore, MD

ThP 268 Identification of MHC Class I Phospho-Peptide Antigens from Breast Cancer Utilizing sHLA Technology and Complementary Enrichment Strategies; Andrew Norris1; A. Michelle English1;Oriana Hawkins2; William Hildebrand2; Jeffrey Shabanowitz1; Donald F. Hunt1; 1University of Virginia, Charlottesville, VA; 2University of Oklahoma Health Sciences Center, Oklahoma, OK

ThP 269 Susceptibility and Innate Resistance: Mass Spectrometry Derived Study of Immune Response to Coccidioidal Infection; Forrest S.E. Helfrich; Lisa F. Shubitz; John N. Galgiani; Vicki H. Wysocki; University of Arizona, Tucson, AZ

ThP 270 A Method to Purify, to Detect and to Identify Specific CDR Peptides by Mass Spectrometry; Lennard Dekker; Lona Zeneyedpour; Martijn van Duijn; Peter Sillevis-smitt; Theo Marten Luider; Erasmus Medical Center, Rotterdam, The Netherlands

ThP 271 Elucidation of Complex Epitopes in Early Discovery Programs Utilizing Epitope Excision, Covalent Modification and Hydrogen-Deuterium Exchange Mass Spectrometry; Shaun Mcloughlin; Maria Argiriadi; Chengbin Wu; Jieyi Wang; Martin Schmidt; Volker Nimmrich; Laura Miesbauer; David Egan; Robert Johnson; Abbott Laboratories, Abbott Park, IL

ThP 272 Exhaustive Identification of Granule Specific Molecules in a Mast Cell Model RBL-2H3 by the live Single-Cell MS; Yuki Yamamoto; Hajime Mizuno; Naohiro Tsuyama ; Takanori Harada; Tsutomu Masujima; Hiroshima Univ. BioMed., Hiroshima, Japan

ThP 273 Expanding the Repertoire of MHC Class II Peptides: Identification of Citrullinated Residues by LC-MS/MS; Manolo D. Plasencia1; Jamie Ireland Rimer1;Shirley J. Petzold1; Henry W. Rohrs2; Michael L. Gross2;Emil R. Unanue1; 1Washington University School of Medicine, St. Louis, MO; 2Washington University, St Louis, MO

ThP 274 Defensin/Cytokine Associations and Response to Vaccination for Typhoid and Cholera: Effects on the Innate Immune System; James Stephenson1; Michael Gardner1; Megan Rowland1; Jonathan Bundy2; Diane Wagener1; Herman Staats3; 1Research Triangle Institute, Durham, NC; 2Centers fro Disease Control, Atlanta, GA; 3Duke University Medical Center, Durham, NC

ThP 275 Identification of Differentially Expressed Host Proteins in Immature and Mature Mycobacterium Tuberculosis Mouse Phagosomes Using O18 Labeling Approach; Janna Kiselar; Ying Wang; Daniela Schlatzer; Lakshmi Ramachandra; Case Western Reserve Univ, Cleveland, OH

ThP 276 Surfaceome Analysis of a T cell Population Using Cell Surface Specific Protein Labeling and Global ICPL-Based Differential Proteomic; Baptiste Leroy1;Nathalie Mari2; Sébastien Denanglaire2; Oberdan Leo2;Ruddy Wattiez1; Fabienne Andris2; 1Dept of Proteomic and Microbiology, UMONS, Mons, Belgium; 2Laboratoire de Physiologie Animale, ULB, Gosselies, Belgium

ThP 277 Characterisation of Protein-Ligand Interaction by SAW-ESI-MS and dPLIMSTEX; Mihaela Dr gu anu1; Brîndu a-Alina Petre1; Tingting Tu2; Don Rempel2; Michael L. Gross2; Michael Przybylski1;1University of Konstanz, Germany, Baden Wuerttemberg; 2Washington University, St. Louis, MO

ThP 278 Subcellular Proteome Profiling of Influenza A Virus Infected Human Macrophages; Niina Lietzén1; Tiina Öhman1; Johanna Rintahaka2; Tero Aittokallio3; Sampsa Matikainen2; Tuula A. Nyman1; 1University of Helsinki, Helsinki, Finland; 2Finnish Institute of Occupational Health, Helsinki, Finland; 3University of Turku, Turku, Finland

ThP 279 A Proteomic Method to Understand Protein Ubiquitination in Mammalian cells; Yi Shi; Jun Qin; Baylor College OF Medicine, Houston, TX

ThP 280 Direct Detection of Antibody/Antigen Complexes by MALDI-TOF Mass Spectrometry: Application to the NCI Clinical Proteomic Technologies for Cancer (CPTC) Initiative; Jack Simpson; Simona Colantonio; Robert Fisher; Gordon Whiteley; Advanced Technology Program, SAIC/NCI-Frederick, Frederick, MD

ThP 281 Therapeutic Metabolomics Identifies Fatty Acid Metabolism as a Therapeutic Target for Treating Multiple Sclerosis; Leah Shriver1; Gary J Patti 2; Bridgit Crews 3; Oscar Yanes4; Gary Siuzdak2; Marianne


Manchester1; 1University of California, San Diego, La Jolla, CA; 2The Scripps Research Institute, La Jolla, CA; 3Millenium Laboratories, San Diego, CA; 4Scripps Research Institute, La Jolla, CA

NUCLEIC ACIDS, 283 - 308 ThP 282 Formation and Recognition of G-Quadruplex

Structure in Proximal Promoter of Vascular Endothelial Growth Factor Gene by ESI Mass Spectrometry; Gu Yuan1; Hui Hui Li2; 1Peking University, Beijing, China; 2Nanjing Normal University, Nanjing, China

ThP 283 Collisionally Activated Dissociation (CAD) and Electron Detachment Dissociation (EDD) for Top-Down Mass Spectrometry of Ribonucleic Acids up to 61 nt; Monika Taucher; Kathrin Breuker; University of Innsbruck, Innsbruck, Austria

ThP 284 Characterization of Interstrand Oligonucleotide Crosslinking Sites of Substituted-Aziridinylbenzoquinones by Infrared Multiphoton Dissociation; Catherine J. Silvestri; Sarah E. Pierce; Frank Guziec Jr.; Lynn Guziec; Jennifer Brodbelt; The University of Texas, Austin, TX

ThP 285 An In-Depth Parallel Study on Using Various MALDI Matrices for MicroRNA Analysis; Chue H. Yang; Dickson M. Wambua; Norman H. L. Chiu; University of North Carolina at Greensboro, Greensboro, NC

ThP 286 Analysis of Dicer-substrate Oligonucleotides in Mouse Liver by Liquid Chromatography/Mass Spectrometry; Kaisheng Jiao; Lou Lamphere; Roberto Guerciolini; Dicerna Pharmaceuticals, Watertown, MA

ThP 287 An LC-Orbitrap Mass Spectrometry Assay for Quantitative Bioanalysis of Therapeutic Small Interfering RNA (siRNA); Randall C. Armour; David J. Borts; Rudy L. Juliano; Michael H. Fisher; Leaf Huang; William C. Zamboni; University of North Carolina, Chapel Hill, North Carolina

ThP 288 Enhanced In-Source Fragmentation in MALDI-MS of Oligonucleotides; Nathan Hagan; Christine Smith; Miquel Antoine; Jeffrey Lin; Andrew Feldman; Plamen A. Demirev; JHU Applied Physics Lab, Laurel, MD

ThP 289 Strategies Towards the Indirect Detection of Therapeutic siRNAs Using Cleavable Small Molecule Mass Tags; Jo-Anne Riley1; Tom Brown1; Graeme T. Clark2; G. John Langley1; 1University of Southampton, School of Chemistry, Southampton, UK; 2Pfizer Global Research and Development, Sandwich, UK

ThP 290 Evaluation of DNA Intercalators Using Electrospray Ionization Tandem Mass Spectrometry; Sarah Pierce;Julia Aponte; Jennifer Brodbelt; The University of Texas, Austin, TX

ThP 291 Probe-Dependent Capture and Analysis of microRNA by MALDI-TOF Mass Spectrometry;Dickson M. Wambua; Chue H. Yang; Norman H. L. Chiu; University OF North Carolina at Greensboro, Greensboro, NC

ThP 292 Mass Spectrometric Analysis of Modified DNA Bases in Telomers; Ilan Vidavsky1; Michael L. Gross1; Phillip Smiraldo2; Woodring E. Wright2; 1Washington University, St Louis, MO; 2University of Texas Southwestern Medical Center, Dallas, TX

ThP 293 Fragmentation of Nucleotides and Nucleosides in a Helium Metastable-Beam Open-Air-Ion-Source Mass Spectrometer; Liang Xue; Matthew Curtis; Mikael A. Minier; Priyanka Chitranshi; O. David Sparkman; Patrick R. Jones; University of the Pacific, Stockton, CA

ThP 294 Synthesis and MS Characterization of Oligodeoxyribonucleotides Containing N6-carboxymethyl-2’-deoxyadenosine and N4-carboxymethyl-2’-deoxycytosine; Jianshuang Wang1;Yinsheng Wang2; 1UC Riverside, Riverside, CA; 2University of California, Riverside, CA

ThP 295 Comparison of Liquid/Liquid and Solid-Phase Extraction for the Quantitative Analysis OF Phosphorothioate Oligonucleotides IN Human Plasma Using LC-MS/MS; Weiwei Yuan; Laixin Wang; Yue Zhao; Yao Shi; Ryan Adler; Min Meng; Scott Reuschel; Patrick Bennett; Tandem Labs, Salt Lake City, UT

ThP 296 LC-MS Analysis of siRNA for the Validation of a Novel Target for Prostate Cancer Therapeutics; A. Cary McGinnis; Buyun Chen; Brian S. Cummings; Michael G. Bartlett; University of Georgia, Athens, GA

ThP 297 Fully Automated Analysis of PCR Products by High Performance ESI-TOF Mass Spectrometry: A Novel Platform for Clinical Research; Jose Gutierrez; Jared Drader; Steven Hofstadler; Ibis Biosciences, Inc., Carlsbad, CA

ThP 298 Differentiation and Sequencing of Three mer-18 DNA Isobaric Oligomers Using Low-Energy CID-MS/MS and the Oligo Mongo Mass Calculator Software; Farid Jahouh; Joseph H. Banoub; MemorialUniversity of Newfoundland, Saint John's, Canada

ThP 299 Analysis and Quantitation of Oligonucleotides Using MALDI TOFTOF; Shixin Sun; Christie L Hunter; Fadi Abdi; Applied Biosystems, Framingham, MA

ThP 300 New Urinary Biomarkers of DNA Damage and RNA Metabolism Using a Non-Target LC-MS/MS Approach; Daniel Beach; Alina Lyagusha; Wojciech Gabryelski; University of Guelph, Guelph, Canada

ThP 301 Evaluating Strong Anion Exchange (SAX) Chromatography for On-Line LC-MS Analysis of Nucleic Acids; Joshua Wilhide; Kevin B. Turner ; Timothy Garrett Jr; Alberto Berton; Daniele Fabris; University of Maryland Baltimore County, Baltimore, MD

ThP 302 Monitoring Changes in the Expression Levels of Transfer RNAs by LC-MS; Siwei Li; Colette Castleberry; Patrick A. Limbach; University of Cincinnati, Cincinnati, OH

ThP 303 ESI-FTICR Evaluation of Recurrent Structural Motifs as Possible Building Modules for Homology Modeling of Ribonucleoprotein Assemblies; Kevin B.Turner; Arie Hawkins; Daniele Fabris; U. of Maryland, Baltimore County, Baltimore, MD

ThP 304 Novel CID and IRMPD Fragmentation of a Hydrated C-glycoside of 5-fluorouridine Formed by the Action of Pseudouridine Synthase RluA; Bogdan Bogdanov1;Edward J. Miracco1, 2; Eugene G. Mueller1; 1University of Louisville, Louisville, KY; 2University of Delaware, Newark, DE

ThP 305 An Easy-to-Use Program for Mass Calculation of Chemically-Modified Oligonucleotides; Fanyu Meng1;Peter Leopold2; Amran Gowani1; Roy Helmy1; Huimin Yuan1; Bing Mao1; 1Merck & Co., Inc, Rahway, NJ; 2BioAnalyte Inc., Portland, ME

ThP 306 Development of Mixed Metal Intercalators and Multifunctional Crosslinkers for in vivo Investigation of Ribonucleoprotein Assemblies; Timothy Garrett Jr;Seungkyung Lee; Kevin B. Turner ; Daniele Fabris; University of Maryland Baltimore County, Baltimore, MD


ThP 307 Novel Isolation and Analysis Method for Oligonucleotide Therapeutics and their Metabolites from Biological Matrices by LC/MS; Michael Mcginley1; Greg Scott1; Mark E. Hail2; 1Phenomenex, Torrance, CA; 2Novatia LLC, Monmouth Junction, NJ

ThP 308 Determination of a Phosphorothioate Oligonucleotide and its 3’n-1 Metabolite by LC-MS from Rat Plasma;Buyun Chen; Michael Bartlett; University of Georgia, Athens, GA

CARBOHYDRATES II, 309 - 326 ThP 309 Development of N-Glycan Serum Library for Rapid

Identification and Quantitation in Disease Biomarker Discovery Using nanoLC/MS; Danielle Aldredge1;Hyun Joo An1; Ning Tang2; Keith Waddell2; Carlito Lebrilla1; 1University of California, Davis, CA; 2Agilent Technologies, Santa Clara, CA

ThP 310 Software-Assisted Peak Annotation and Isomer Detection for Oligosaccharide Mass Spectra: A Case Study; David Ashline1; Jake Fournier1; Tonya Second2;1Glycome Technologies Inc., Lee, NH; 2Thermo Fisher Scientific, San Jose, CA

ThP 311 Permethylated Oligosaccharide Isomers Elucidation by Polymeric Porous layer Open-Tubular Nanoliquid Chromatography/Tandem Mass Spectrometry; Ming-Yi Ho; Chung-Lin Liao; Genomics Research Center, Academia Sinica, Taipei, Taiwan

ThP 312 RP-nano-LC-ESI-MS/MSMS of Permethylated Glycans and Data Analysis Thereof; Ilja Ritamo1;Hannu Peltoniemi2; Suvi Natunen1; Jarkko Räbinä1;Leena Valmu1; 1Finnish Red Cross Blood Service, Helsinki, Finland; 2Applied Numerics Ltd, Helsinki, Finland

ThP 313 Structure Analysis of Derivatized Chondroitin Sulfate Oligosaccharides Using Reverse Phase LC/MS; Rongrong Huang; Joshua S. Sharp; University of Georgia, CCRC, Athens, GA

ThP 314 Fragmentation Behavior of Oligosaccharides Using the MALDI Orbitrap and 3-Aminoquinoline as Matrix and Derivatizing Agent; Marion Rohmer1;Bernd Stahl2; Ute Bahr1; Michael Karas1; 1GoetheUniversity, Frankfurt Am Main, Germany; 2DanoneResearch Centre for Specialised Nutrition, Friedrichsdorf, Germany

ThP 315 Investigations of Structures and Fragmentation Pathways by Electron Transfer Dissociation of Permethylated Milk Sugars; Liang Han1; Catherine E. Costello2; 1Boston University, Boston, MA; 2Boston University School of Medicine, Boston, MA

ThP 316 Multivariate Analysis Of Electron Detachment Dissociation (EDD) Mass Spectra For Heparan Sulfate (HS) Tetrasaccharides Differing Only In Hexuronic Acid Stereochemisty; Han Bin Oh1;Franklin E. Leach Iii2; Sailaja Arungundram2; Kanar Al-Mafraji2; Andre Venot2; Geert-Jan Boons2; Jon Amster2;1Sogang University, Seoul, South Korea; 2University of Georgia, Athens, GA

ThP 317 Electron Detachment Dissociation (EDD) of Fluorescently Labeled Oligosaccharides; Wen Zhou;Kristina Hakansson; University of Michigan, Ann Arbor, MI

ThP 318 Electron Induced Dissociation (EID) of Singly Protonated Glycans; Di Gao; Wen Zhou ; Kristina Hakansson; University of Michigan, Ann Arbor, MI

ThP 319 Electron Detachment Of Chondroitin Glycosaminoglycan Carbohydrates; Mellisa Ly1;Franklin E. Leach III2; Tatiana Laremore1; Jeremy

Wolff3; Robert J. Linhardt1; Jon Amster2; 1Renesselaer Polytechnic Institute, Troy, NY; 2University of Georgia, Athens, GA; 3Bruker Daltonics, Billerica, MA

ThP 320 Profiling Fetuin Glycans Composition by HILIC-LC/MSE; Vera Ivleva 1; Ying-Qing Yu 1; Jonathan Bones2; Martin Gilar1; 1Waters Corporation, Milford, MA; 2Conway Institute, University College Dublin, Dublin, Ireland

ThP 321 Metal Adduction Has a Remote Effect in the Electron Capture Dissociation of Permethylated Glycans;Xiang Yu; Yiqun Huang; Cheng Lin; Catherine Costello; Boston University, Boston, MA

ThP 322 Alternative to Chemical Denaturation for Efficient Deglycosylation of glycoproteins – Applications for Characterization of Vaccines Glycoproteins by Mass Spectrometry; Jean Dubayle; Sandrine Vialle; Philippe Talaga; Biochemistry Research, Campus Mérieux, Marcy L'etoile, France

ThP 323 Distinguishing HMOS Pure Isomers and Mixtures by IRMPD MALDI-FTMS; Carlito Lebrilla2; Andrés Guerrero1; Juan Davalos1; 1Rocasolano Physical Chemistry Institute, Madrid, Spain; 2University of California, Davis, CA

ThP 324 Lactose Determination in Low Lactose and Lactose Free Dairy Products by ZIC-HILIC-LC-MS; Petrus Hemstrom; Patrik Appelblad; MerckSeQuant AB, Umea, Sweden

ThP 325 A Study on Structural Analysis of Neutral Oligosaccharides by Using MALDI-FT ICR Mass Spectrometry; Shuying Liu; Hongmei Yang; Changchun Inst Appl Chem, Changchun, China

ThP 326 Differentiation of Stereochemistry and Anomeric Configuration of Structurally Diagnostic Carbohydrate Anions, m/z 221, by Infrared Photodissociation at 10.6 Microns; Brad Bendiak;Tammy Fang; University of Colorado Health Sciences Center, Aurora, CO

LIPIDS III, 327 - 346 ThP 327 Analysis of Urinary Isoprostanes by Chiral Liquid

Chromatgraphy-Electron Capture Atmospheric Pressure Chemical Ionization/Mass Spectrometry;Clementina Mesaros1; Seon Hwa Lee 2; Ian A. Blair3;1UPenn, Philadelphia, PA; 2Tohoku University, Sendai, Japan; 3Univ. of Penn/SOM/Pharmacol, Philadelphia, PA

ThP 328 Tandem Mass Spectrometric Analysis of Oxidized Glycerophospholipids by MALDI Using a Linear Ion Trap; Whitney L. Stutts1; Kerolyn S. Valente2; Timothy J. Garrett3; Richard A. Yost1; 1Department of Chemistry, University of Florida, Gainesville, FL; 2Department of Biochemistry,University of Sãn Paulo, Sãn Paulo, Brazil; 3College of Medicine, University of Florida, Gainesville, FL

ThP 329 Imaging and Structural Analysis of Phospholipids and Sulfatides in Brain Lipid; Alice Delvolvé; Benoit Colsch; Amina S Woods; NIH/NIDA/IRP, Baltimore, MD

ThP 330 An Orthogonal Approach to Lipidomic Analysis by Fraction Collection Followed by Ion Mobility Mass Spectrometry; Jose Castro-Perez; David G. McLaren; Paul Miller; Doug Johns; Sheng-ping Wang; Brian K. Hubbard; Thomas P. Roddy; Merck Co & Inc, Rahway, NJ

ThP 331 Label free Differential Lipid Analysis For Rat Brain Samples Affected By Stroke Using a Linear Ion Trap Orbitrap Mass Spectrometer; Sucharita Dutta1; Alice


Delvolve2; Shelley N Jackson2; Mikko Airavaara2;Brandon K. Harvey2; Yun Wang2; Amina S. Woods 2;1Thermo Fisher, San Jose, CA; 2NIDA-IRP, NIH, Baltimore, MD

ThP 332 Complementary Acquisition Strategies for Comprehensive Lipid Profiling of Microbes for Biofuel Applications; Sahana Mollah1; Eva Duchoslav 2; Brigitte Simons2; 1AB Sciex, Foster City, CA; 2AB/Sciex, Concord, ON

ThP 333 A Strategy For The Quantitative Analysis of All Lipids With A Single Acquisition Method; Brigitte Simons; Stephen Tate; AB Sciex, Concord, Ontario

ThP 334 Quantitation of Ceramides in Plasma and Tissues Using HPLC On-Line Electrospray Ionization Tandem Mass Spectrometry; Renliang Zhang; Yoon-Mi Chung; Hazel Huang; Takhar Kasumov; John Kirwan; Cleveland Clinic, Cleveland, OH

ThP 335 Quantification of Triacylglycerols Regioisomers Present in Lipid Extracts by CID and CID/OzID Techniques; Huong Pham Thu1; Stephen Blanksby1;Todd Mitchell2; 1School of Chemistry, University of Wollongong, Australia; 2School of Health Sciences, University of Wollongong, Australia

ThP 336 Quantitative Analysis of 1-Deoxydihydroceramides Using Normal Phase Liquid Chromatography Coupled to a Quadrupole Linear-ion Trap Mass Spectrometer; Sibali Bandyopadhyay1; Hyejung Park2;Rebecca Shaner1; Christopher A. Haynes1; Mark Cameron Sullards1; Alfred H. Merrill1; 1GeorgiaInstitute of Technology, Atlanta, GA; 2Boston University School of Medicine, Boston, MA

ThP 337 Quantitative Analysis of Phospholipids Using NALDI Target Plates; Simona Colantonio1; Jack Simpson1;Robert J. Fisher1; Anu Puri2; Amichai Yavlovich2;Robert Blumenthal2; Julie M. Belanger2; 1SAIC/NCI-Frederick, Frederick, MD; 2National Cancer Institute, Frederick, MD

ThP 338 Development of Global and High Sensitive 2D-Phospholopidomics; Yoshiaki Sato1; Tatsuji Nakamura1; Ken Aoshima 1; Keith Wilcoxen2; Yoshiya Oda2; 1Eisai Co., Ltd, Tsukuba, Japan; 2Eisai Inc., Andover, MA

ThP 339 Targeted Metabolomics - Validated Analysis of Free and Bonded Fatty Acids in Biofluids without Fractionation Using GC-MS After Methylation; HaiPham Tuan; Stephanie Angeben; Therese Koal; Biocrates Life Sciences AG, Innsbruck, Austria

ThP 340 Targeted Lipidomics – High-Throughput Analysis of Lipid Metabolites in Biological Samples via Flow Injection ESI-MS/MS; Diane Schmiederer; Hai Pham Tuan; Doreen Kirchberg; Therese Koal; Biocrates Life Sciences AG, Innsbruck, Austria

ThP 341 Application of Supercritical Fluid Chromatography/Mass spectrometry to Lipid Profiling of Soybean; Takeshi Bamba 1; Jae Won Lee1;Takato Uchikata1; Atsuki Matsubara1; Takuji Nakamura2; Eiichiro Fukusaki1; 1Dept.Biotech., Grad. Sch.Eng., Osaka Univ., Suita, Japan; 2National Institute of Crop Science, Naro, Tsukuba, Japan

ThP 342 Development of LC-MRM Method for a Rapid Screening of Rhamnolipids Produced by Pseudomonas Strains; Leonardo Sastoque-Cala1, 3;Cecilia Silva-Sanchez2; Refugio Rodriguez-Vazquez2;Alba Marina Cotes-Prado3; Aura Marina Pedroza-Rodriguez1; 1Pontificia Universidad Javeriana, Bogota,

Colombia; 2CINVESTAV-IPN, Mexico, D.F., Mexico; 3corpoica, Bogota, Colombia

ThP 343 Integrated MS2/MS3 Spectra Database Facilities Data-Dependent Mouse Heart Cardiolipin Analyses with the LipidQA Software Platform; Haowei Song;Jack Ladenson; John Turk; Washington University school of medicine, St. Louis, MO

ThP 344 Lipid Identification Using a MS/MS Database of 120,000 Tandem Mass Spectra; Tobias Kind; Kwang-Hyeon Liu; Do Yup Lee; Oliver Fiehn; UC Davis - Metabolomics, Davis, CA

ThP 345 Spectral Processing of QQQ Scans for Lipidomics Preserving All Features in a Spectrum; Todd Williams1; Lawrence Seib1; Mary Michaelis1; Asma Zaidi2; 1University of Kansas, Lawrence, KS; 2Kansas City University of Medicine and Biosciences, Kansas City, Missouri

ThP 346 Benchmarking of Shotgun Lipidomics Software tools;Ronny Herzog1, 3; Dominik Schwudke 2; Andrej Shevchenko1, 3; 1Max Planck Institute CBG, Dresden, Germany; 2National Centre for Biological Sciences, Tata Inst, Bangalore / Bengaluru, India; 3Max Planck Institute CBG, Dresden, Germany

SMALL MOLECULE QUANTITATION III, 347 - 375 ThP 347 Determination of Metformin in Rat Plasma by

HILIC-MS/MS Combined with Tecan Automation and Direct Injection; Wei Zhang1; Futian Han 1; Harry Zhao1; Zhongping John Lin1; Qingtao (Mike) Huang2;Naidong Weng2; 1Frontage Laboratories, Inc., Malvern, PA; 2Johnson & Johnson Pharmaceutical Research & Dev, Raritan, NJ

ThP 348 Determination of Metformin in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry; Ang Liu; Stuart Coleman; Matrix BioAnalytical Laboratories, New Haven, CT

ThP 349 Simultaneous Determination of Bezafibrate and Diflunisal in Human Plasma Using Liquid Chromatography-Tandem Mass Spectrometry; Ang Liu; Stuart Coleman; Matrix BioAnalytical Laboratories, New Haven, CT

ThP 350 Validation of an LC/MS/MS Method for the Determination of Ibuprofen in Miniature Swine Plasma; Lawrence Andrade; Adam Grenier; Teresa Pekol; Synomics Pharma, Wareham, MA

ThP 351 New Method for Rapid detection of Low Levels of Beta-Lactam Antibiotics in Recombinant Protein Therapeutics; Melissa Zolodz; James Carroll; Ned Mozier; Pfizer, Chesterfield, MO

ThP 352 Quantification of a Very Polar Small Molecule, Guanfacine, in Human Plasma Using an Acquity UPLC and a 50 pg/mL LLOQ; Troy Voelker; Lin Tan; Min Meng; Spencer Carter; Scott Reuschel; TandemLabs, Salt Lake City, UT

ThP 353 Simultaneous Determination of Naloxone, 6 -naloxol,and Naloxone-3 -D-glucuronide in Mouse Plasma Using Liquid Chromatography-Tandem Mass Spectrometry; Hongliang Jiang1; Manjunath Shet2;Yurong Wang1; 1Covance Laboratories Inc., Madison, WI; 2Purdue Pharma L.P., Stamford, CT

ThP 354 Full Validation of LC-MS/MS Method for Determination of Phloroglucinol in Human Plasma;Jin young Kim1; Hyeon Jin Bae1; Sun Koung Joung1;Kyung Hee Cho1; Sookie La1; Hee Joo Lee1, 2; 1BioCoreCo.,Ltd, Seoul, Korea; 2Seoul Clinical Laboroatories, Seoul, Korea


ThP 355 Quantification of Bambuterol in Human Plasma by LC-MS/MS Using Electrospray Ionization Sources;Ju-Hong Lee; Eunmi Ban; Jong-Oh Lee; Bio-Medieng,Seongnam, South Korea

ThP 356 Sensitive HPLC/MS/MS Assay for Bioequivalence Test of Anastrozole and Letrozole of Anti-Estrogen in Human Plasma; Bohee Lim1; Jooyeon Park1; Haesook Bok1; Soo-Youn Lee2, 3; Wooseong Huh1, 5; Hojoong Kim1; Sung Hwa Hong1; Jaewook Ko1, 4; 1Clinical Trial Center,Samsung Medical Center, Seoul, South Korea; 2Department of Laboratory Medicine and Genetics, Samsung Medical Center,Seoul, South Korea; 3Sungkyunkwan University School of Medicine, Seoul, South Korea; 4Division of Clinical Pharmacology, Seoul, South Korea; 5Department of Medicine,Samsung Medical Center, Seoul, South Korea

ThP 357 Sensitive Assay for Determination of Entecavir in Human Plasma Using LC/MS-MS; Yifei Liu; Erika Hess; Tandem Labs, a division of Labcorp, West Trenton, NJ

ThP 358 Quantitative Analysis of Sirolimus in Mouse Whole Blood Using UPLC-MS/MS; Linh Tran; Hongyan Li; Mark Rose; Christopher A. James; Amgen, Inc., Thousand Oaks, CA

ThP 359 Quantification of Tacrolimus in Human Whole Blood by Dry Blood Spot (DBS)-LC-MS/MS; Haiqing Ding;Kathryn Piening; Tian-Sheng Lu; Guangchun Zhou; Yan Xu; John-Paul Gutierrez; Kristin Miller; Yong-Xi Li; Medpace Bioanalytical Laboratories, Cincinnati, OH

ThP 360 A Simple and Sensitive Method for the Determination of Cinacalcet in Human Dried Blood By Reverse Phase LC-MS/MS; Philip S. Wong; Bernd Bruenner; Christopher James; Amgen, Thousand Oaks, CA

ThP 361 Determination of p-Phenylenediamine in Hair dyes by Using Microwave-Assisted Derivatization and Gas Chromatography-Mass Spectrometry; Tzu-Ying Lin; Jing-Yi Huang; Maw-Rong Lee; National Chung-Hsing University, Taichung, Taiwan

ThP 362 In vivo Determination of Polyamines in Human Serum by Using Liquid Chromatography-Tandem Mass Spectrometry; Ling-Chun Liao1; Soo-Ray Wang2;Maw-Rong Lee1; 1National Chung-Hsing University, Taichung, Taiwan; 2Chung Shan Medical University, Taichung, Taiwan, ROC

ThP 363 Determination of Leukotriene B4 in Human Plasma by UFLC-MS/MS – A Novel Approach to Evaluate LTB4 as a Pharmacodynamic Biomarker; Qingtao (Mike) Huang1; Naidong Weng1; Futain Han2; Weisheng Lin2; Zhongping (John) Lin2; Hsiaoju Lin2; Anne Fourie1; Xiaohua Xue1; Kirk Bertelsen1; Jan de Jong1;Ann Welton1; 1Johnson & Johnson, Princeton, NJ; 2Frontage Lab, Marven, PA

ThP 364 Determination of Serum Ribavirin Content Using Tandem Mass Spectrometry (LC-MS/MS): An Accurate, Precise, Selective and Sensitive Method;Claudia Meek 1; Mamta K. Jain2; Tawanda Gumbo2;Richard Leff1, 2; 1Texas Tech University Health Sciences Center, Dallas, TX; 2University of Texas Southwestern Medical Center, Dallas, TX

ThP 365 Differential Isotope Labeling for Quantitation of Acylglycines in Human Urine; Avalyn Lewis; Liang Li; University of Alberta, Edmonton, Canada

ThP 366 A Rapid and Sensitive Chiral LC-MS/MS Method for the Determination of Warfarin Enationmers in Human Plasma; Chen-Yu Wang; Yan Xu; John Paul

Gutierrez; Kristin Miller; Yong-Xi Li; Medpace Bioanalytical Laboratories, Cincinnati, OH

ThP 367 A Validated Method by LC-MS/MS with Electrospray Ionization for Chiral Determination of R-Lipoic Acid and S-Lipoic Acid in Human Plasma;Hwa Suk Kim; Seo Hyun Yoon; Joo-Youn Cho; Kyung Sang Yu; In-Jin Jang; Seoul National University, Seoul, South Korea

ThP 368 GLP Validation for a Quick and Sensitive Analysis of Lidocaine in Rat and Minipig Plasma; Matthew Pollard; Derek Laine; Chad Christianson; Shane Needham; Alturas Analytics, Inc., Moscow, ID

ThP 369 Quantification of Caffeine Metabolites in Human Urine via a Simple and Rapid Liquid Chromatography-Tandem Mass Spectrometry Method; Yao Shi; Laixin Wang; Jian Chen; Lisa Rohde; Spencer Carter; Min Meng; Scott Reuschel; Patrick Bennett; Tandem Labs, Salt Lake City, UT

ThP 370 Validation of a Liquid Chromatography Tandem Mass Spectrometry Assay Method for the Determination of R-Modafinil in Human Plasma;Gina de Boer; Xiufeng Ji; Winnie Lui; Rong Yi; Grace van der Gugten; Xuejun Peng; Can Test Ltd, Burnaby, BC, Canada

ThP 371 Comprehensive Typing of Unknown Microorganisms Based on Quantitative Analysis of Targeted Long Chain Fatty Acids by Mass Spectrometry; Hongying Zhong; Central China Normal University, Wuhan, China

ThP 372 Quantitative Analysis of HM30181A in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS); Bora Kim; Seul Oh ; Seo Hyun Yoon; Joo-Youn Cho; Kyung-Sang Yu; In-Jin Jang; Seoul National university, Seoul, Republic of Korea

ThP 373 Matrix Effect Correlation for Multiple Analytes Using a Single Internal Standard; Masuyuki Sugiyama; Shuhei Hashiba; Kazuki Tanaka; Hideki Hasegawa; Yuichiro Hashimoto; Hitachi, Ltd, Central Research Lab, Kokubunji, Tokyo, Japan

ThP 374 Relative Noise: A Novel Method for Determining Signal to Noise Ratios for Peaks in Quantitative LC-MS Applications; Gordana Ivosev; Ron Bonner; Lyle Burton; John Gibbons; AB SCIEX, Concord, Canada

ThP 375 Simultaneous Quantitative Determination of Terbutaline and Its Prodrug Bambuterol in Human Plasma; Jong-Dae Kim1; Sun Koung Joung1; Kyung Hee Cho1; Sookie La1; Hee Joo Lee1, 2; 1BioCoreCo.,Ltd, Seoul, Korea; 2Seoul Clinical Laboratories, Seoul, Korea

SMALL MOLECULE ANALYSIS, 376 - 408 ThP 376 Separation and Analysis of Fungicides in Orange Peel

by GC/API-LC/MS; Sheher Bano Mohsin1; Michael Woodman1; Harry Prest2; Patrick D. Perkins1; 1Agilent Technologies, Schaumburg, IL; 2Agilent Technologies, Inc, Santa Clara, CA

ThP 377 Enhanced Reproducibility of Small Molecule Analysis by MALDI-TOF Mass Spectrometry;Hyesun Maeng1, 2; Miyoung Ha1; Yangsun Kim1, 2;1Hudson surface Technology, Inc., Newark, DE; 2Applied Surface Technologly, Inc., Suwon, South Korea

ThP 378 3D Imaging Mass Spectrometry of the Heart; Lara Fornai1, 2; Ivo Klinkert1; Annalisa Angelini2; Frans Giskes1; Lennaert A. Klerk1; Marny Fedrigo2; Gaetano Thiene2; Ron M.A. Heeren1; 1Amolf, Amsterdam, Netherlands; 2University of Padua Medical School- Cardiovascular, Padua, Italy


ThP 379 A Single Solution to Walk-Up Mass Spectrometry Access and Data Presentation; Julie Herniman; G. John Langley; University of Southampton, Southampton, UK

ThP 380 Identification of Clozapine Oxidation and Hydrolysis Degradation Products by Using UPLC/UV/ESI-MS;Xia Zhang; Xiaoping Zhang; Wai Tsui; Xiaotang Huang; Teva Pharmaceuticals, Montvale, NJ

ThP 381 Structure Elucidation with an Ultra-High Resolving TOF Instrument by Alternating MS and Broad-Band CID Analyses; Peter Sander; Ilmari Krebs; Sebastian Goetz ; Birgit Schneider; Bruker Daltonik, Bremen, Germany

ThP 382 Detection of Small Biological Molecules by LDI-TOF-MS Using Self-Assembled Molecules on Silicon Surfaces; Ömür Çelikbçak1; Gökhan Demirel2; Erhan Pi kin1; Bekir Salih1; 1Hacettepe University, Ankara, Turkey; 2Gazi University, Ankara, Turkey

ThP 383 GC/MS Analysis of Bisphenol A and Other Potential Leachables from Container Closure Systems Used for Biopharmaceutical Therapeutics; Sherry Castle; May Joy Miller; Katherine Taylor; Vinh Nguyen; Shire Human Genetic Therapies, Cambridge, MA

ThP 384 Development of a Universal Method for High-Throughput Screening of Antihypertensive Drugs by MALDI MS; Eduardo C. Dias; Joey C. Latham; Dan M. Roden; Daniel R. Masys; Nancy J. Brown; Richard M. Caprioli; Vanderbilt University, Nashville, TN

ThP 385 Chemically Modified Porous Silicon for Laser Desorption/Ionization Mass Spectrometry; Irina Shmigol1; Sergei Alekseev2; Vladimir Zaitsev2; Valeriy Pokrovsky1; 1O.O. Chuiko Institute of Surface Chemistry NASU, Kiev, Ukraine; 2National Taras Shevchenko University, Kiev, Ukraine

ThP 386 Simultaneous Quantitation of Free and Liposomal Drug Forms in Human Serum by Evaporation-Free Extraction; François Viel; Nuno Santos; Aimin Tan; Lise Laforest; Nadine Boudreau; Ann Lévesque; Adrien Musuku; Robert Massé; Anapharm, Québec, Canada

ThP 387 Electron Induced Dissociation (EID) of Small, Singly Charged Ions of Pharmaceutical Importance; Jackie Mosely1; Michael Smith1; Zied Kaabia1; Aruna Prakash1; Glenn Hurst1; Martin Sims2; Anthony W.T. Bristow2; 1Durham University, Durham, UK; 2AstraZeneca, Macclesfield, UK

ThP 388 Relative Instability of Deuterated Internal Standard under Different pH Conditions and According to Deuterium Atoms Location; Caroline Savard; Nathalie Pelletier; Nadine Boudreau; Sylvain Lachance ; Ann Lévesque; Robert Massé; Anapharm, Québec, Canada

ThP 389 Quantitation of Testosterone and Other Keto Steroids in Human Serum Using Derivatization Chemistry and LC/MS/MS; Michal Weinstock1; Brian Williamson2; Babu Purkayastha3; 1AB SCIEX, Framingham, MA; 2AB, Framingham, MA; 3AppliedBiosystems, Framingham, MA

ThP 390 Sample Collection Stability Issue When Dealing with Highly Bounded to Erythrocytes Molecules; Nadia Savard; Marie-Josée Marcoux; Sylvain Lachance ; Ann Lévesque; Robert Massé; Anapharm, Québec, Canada

ThP 391 ESI HRMSn Fragmentation Pathways of Some N-Heterocyclic Drug Compounds; Claudio Medana; University of Turin, Torino, Italy

ThP 392 Mass Spectra from Dried Fullerene C60 Solutions Correlate with Aggregation in Initial Samples; Sergei Snegir1; Valeriy Pokrovsky1; Michael Avdeev2; Alena

Kyzyma2; 1O.O. Chuiko Institute of Surfase Chemistry, NASU, Kiev, Ukraine; 2Joint Institute for Nuclear Research, Dubna, Russia

ThP 393 Rapidly Monitoring Chemical Reactions and Identifying Products with Thermal Desorption/MS to Improve Synthesis Lab Workflow and Productivity;Peter J. Lee; Michael P. Balogh; Jennifer Burgess; Waters Corporation, Milford, MA

ThP 394 A Robust and Ultra Sensitive Low Picograms Hormonal Contraceptive Determination by LCMSMS; Guy Havard; Sylvain Lachance ; Ann Lévesque; Robert Massé; Anapharm, Québec, Canada

ThP 395 Infrared Laser Desorption Electron Ionization for Ambient Mass Spectrometry; Juaneka Hayes; Sung Gun Park; Kermit K. Murray; Louisiana State University, Baton Rouge, LA

ThP 396 Analysis of Urinary 8-Oxo-7,8-dihydro-2`-deoxyguanosine by Liquid Chromatography/Mass Spectrometry; Ashley Wohler1; Dipti Mangal1;Clementina Mesaros2; Laura Phillips3; Ian A. Blair4;1University of Pennsylvania, Philadelphia, PA; 2UPENN, Philadelphia, PA; 3Agilent Technologies, Wilmington, DE; 4Univ. of Penn/SOM/Pharmacol, Philadelphia, PA

ThP 397 WithdrawnThP 398 Structure Elucidation Methodologies for Oxygen-

and Methyl-Containing Aromatic Analytes via Positive-Mode APCI/MSn Coupled with HPLC;Lucas Amundson1; Vanessa Gallardo1; Steven Habicht1,

2; Nelson Vinueza1; Ryan Shea3; Allen Mossman3;Hilkka Kenttamaa1; 1Purdue University, West Lafayette, IN; 2CNA, Alexandria, Virginia; 3BP Chemicals, Naperville, IL

ThP 399 Inter-Laboratory Validation of a Modified QuEChERS and LC-MS/MS Method for Analysis of >200 Pesticide Residues in Six Fatty Food Products;Yi Lin1; Narong Chamkasem2; Kai Chang3; Jon Wong3;1FDA PRLSW, Irvine, CA; 2FDA SRL, Atlanta, GA; 3FDA CFSAN, College Park, MD

ThP 400 Investigation of Luciferin Analogs Using LC-UV-MS/MS; Pegah Jalili; Ettigounder Ponnusamy; Kevin Ray; Gordon Nicol; Sigma-Aldrich, St. Louis, MO

ThP 401 Exctraction of Putative Endocrine Disruptors from Maternal Serum and Fetal Meconium and Detection by ESI Tandem MS; Stephanie Curtice; Jeffrey May; Michael J. Van Stipdonk; Wichita State University, Wichita, KS

ThP 402 Identification of Degradants of Sufetanil Under Oxidative and Thermal Stress Using LC/MS/MS and UV Detection; Renee Huang; Loren Olson; AB Sciex, Foster City, CA

ThP 403 In-Depth Fragmentation Analysis Utilizing Accurate Mass Q-ToF MS/MS for Identification of Degradation Products of Fluphenazine Decanoate in an Oil-based Matrix; Todd Schwier; Paul Bigwarfe; Esther Hwang; Samantha Leidner; Hospira, Inc., Lake Forest, IL

ThP 404 Application of Desorption Electrospray Ionization to the Analysis of Liquid Crystal Displays; Sung-Chan Jo; LCD R&D Center, Samsung Electronics, Co., Ltd., Yongin, South Korea

ThP 405 LD-FTICR for Exact Molecular Determination of Endohedral Metallofullerenes; Michael Easterling1;Steven Stevenson2; Christopher Thompson 3; 1Bruker Daltonics, Inc., Billerica, MA; 2University of Southern


Mississippi, Hattiesburg, MS; 3Bruker Daltonics Inc., Billerica, MA

ThP 406 High-throughput Analysis of Keto-eicosanoids in a COX-2 Inhibition Assay via MALDI MS; Joey C. Latham; Joseph Manna; Philip Kingsley; Lawrence Marnett; Richard M. Caprioli; Vanderbilt Univ Sch of Med, Nashville, TN

ThP 407 Multi-Residue Analysis of Veterinary Drugs in Milk by Turbulent Flow Chromatography Tandem Mass spectrometry; Marie-Hélène Le Breton; NestléResearch Center, Lausanne, Switzerland

ThP 408 Accurate Mass Measurements: Identifying Known Unknowns Using Publicly Accessible Databases;James L. Little1; Curtis D. Cleven1; Stacy C. Brown2;1Eastman Chemical Company, Kingsport, TN; 2East Tennessee State Univ., Johnson City, TN

LC/MS: 409 - 436 ThP 409 Development of a Method for the Quantification of

Fluorouracil and Flucytosine in Human Plasma; Lee Winchester; John Rollag; Kayan Harris; Bridget Prenosil; MDS Pharma Services, Lincoln, NE

ThP 410 Novel Comprehensive On-line Two-dimensional Liquid Chromatography of Peptides by HILIC-RP-ESI/MS for Detailed Protein Characterization;Krishnamoorthy Kuppannan; Scott Young; The Dow Chemical Company, Midland, MI

ThP 411 Dried Blood Spots Sampling in Combination with LC-MS/MS for Quantitative Analysis of Metoprolol Enantiomers and its Active Metabolite O-Desmethyl Metoprolol; Xiaorong Liang; Hongliang Jiang; Xi Chen; Tom Addison; Kevin Jones; Douglas Fast; Covance Laboratories Inc., Madison, WI

ThP 412 Factorial Design for Optimizing the Q-TOF Setting. Reproducibility of Nanospray Measurements and the Development of MS/MS Libraries; Yamil Simón-Manso; Pedatsur Neta; Xiaoyu Yang; Stephen E. Stein; NIST, Gaithersburg, MD

ThP 413 Calibration of User-Defined LC/MS Systems for Complementary Filtering and Validation of MS Based Identifications by “predictive” Chromatography of Biomacromolecules; Anton Goloborodko1; Tatyana Perlova1; Marina L. Pridatchenko1; Alexander V. Gorshkov2; Irina A. Tarasova1; Eugene Moskovets4; Mikhail V. Gorshkov1;Alexander R. Ivanov 3; 1Institute for Energy Problems of Chemical Physics, Moscow, Russian Federation; 2Institute of Chemical Physics, Moscow, Russian Federation; 3Harvard University HSPH, Boston, MA; 4MassTech Inc., Columbia, MD

ThP 414 LC-MS with Integrated UV Detection for Mass Balance Studies; Carmai Seto1; Elizabeth Kwong2;Takeo Sakuma1; 1AB SCIEX, Concord, CANADA; 2Merck and Co, Rahway, New Jersey

ThP 415 Quantitative Determination of Bisphenol A from Human Saliva by Bulk Derivatization and Trap-and-Elute HPLC-ESI-MS; Samuel H. Yang1; Aaron A. Morgan1; Hien P. Nguyen1; Benjamin J. Figard2; Kevin Schug1; 1University of Texas at Arlington, Arlington, TX; 2Shimadzu North America (Regional Office - Houston), Houston, TX

ThP 416 Determination of Vancomycin in Human Plasma Using LC/MS-MS; Yifei Liu; Erika Hess; TandemLabs, a division of Labcorp, West Trenton, NJ

ThP 417 A New LC-MS Method for Investigating the Gamma-Aminobutyric Acid (GABA) Shunt Pathway in Plants; Stephen J. Ambrose1; Natalia Rudnitskaya2;

Mark Pickard2; Randall Purves1; 1National Research Council, Saskatoon, Canada; 2InfraReady Products Ltd., Saskatoon, Canada

ThP 418 Matrix Effect and Correction in the Analysis of Bile Acids Composition for Pouchitis by LC-ESI-MS;Xiaohan Cai1; Xiang Zhou1; Bo Shen2; Baochuan Guo1;1Cleveland State University, Cleveland, OH; 2Cleveland Clinic, Cleveland, OH

ThP 419 Femtogram Quantitation of Naltrexone and 6- -Naltrexone in Human Plasma by LC-MS/MS; Roger D.D. Demers; Angela Lynn McGrath; Tandem Labs, West Trenton, New Jersey

ThP 420 Next-Generation Autosampler for LC-MS Equipped with Straight Injection Technology TM; Yoshikazu Sugito; Tsuneaki Kaneko; Osamu Shirota; Shiseido Co. Ltd, Tokyo, Japan

ThP 421 Quantitation of Tetracycline and 3 Derivatives in Human Urine by LC-MS/MS; Roger Demers; Daria Wentzel; Denny Yifei Liu; Laura Cojocaru; Erika Hess; Heike Mongiovi; Tandem Labs, West Trenton, NJ

ThP 422 Matrix Blind Online LC Sample Extraction Coupled with High Resolution Benchtop Mass Spectrometry Detection for Food Matrices; Yang Shi; Catherine Lafontaine; Francois A. Espourteille; Thermo Fisher Scientific, Franklin, MA

ThP 423 Comparison of Full Scan High Resolution MS and Triple Quadrupole SRM in Quantitative Bioanalysis;Yuan-Qing Xia1; Jim Lau2; Bob Walker2; Timothy Olah1; Mohammed Jemal1; 1Bristol-Myers Squibb Company, Princeton, NJ; 2Agilent Technologies, Inc, Wilmington, Delaware

ThP 424 Spacio-temporal Molecular Distribution in a Developing Frog (Xenopus) Egg by Micro LC-MS;Siyu Zhang1; Atsushi Kurisu1; Naohiro Tsuyama 1;Hajime Mizuno1; Atsushi Suzuki2; Kimiko Takebayashi-Suzuki2; Tsutomu Masujima1; 1Hiroshima Univ. BioMed., Hiroshima, Japan; 2Hiroshima Univ. Sci., Higashihiroshima, Japan

ThP 425 Use of Orthogonal Sub-2 m Diphenyl LC Columns for Improved Sensitivity in Bioanalysis; Mike Chang;David Jones; Norwin von Doehren; Wilroy Bennen; Ritu Arora; Varian Inc., Lake Forest, CA

ThP 426 LC-ESI-MS/MS Analysis of Conjugated Steroid Estrogens for Understanding the Drug-Induced Modulation of Sulfotransferase Activity; Sergiu P.Palii; Margaret O. James; Sriram Ambadapadi; Laura Rowland-Faux; University of Florida, Gainesville, FL

ThP 427 An LC-MS/MS Assay for the Analysis of Hexamethylene Bisacetamide in a Human Breast Cancer Mouse Model; Kerri M. Smith1; Wannarasmi Kechart2; Monica Montano2; Yan Xu1, 3; 1ClevelandState University, Cleveland, OH; 2Case Western Reserve University, Cleveland, OH; 3Case Cancer Pharmacology Core Facility, CWRU, Cleveland, OH

ThP 428 Determination of Relative Affinity and Inhibitory Activity of Pyrazolone Analogs Using Mycobacterium Tuberculosis Shikimate Kinase LC-MS Based Screening Assays; Vanisree Mulabagal; Angela Calderon; Auburn University, Auburn, AL

ThP 429 Rapid Through-put Methods for Accurate Quantification of Oligosaccharides in Human Milk;John S. Strum1; Jaehan Kim2; Shuai Wu1; Mariana Barboza1; Maria Lorna A. de Leoz1; Rudolf Grimm3;Carlito B. Lebrilla1, 4; 1Deparment of Chemistry, UC Davis, Davis, CA; 2Department of Viticulture and Enology, UC Davis, Davis, CA; 3Agilent Technologies,


Inc., Santa Clara, CA; 4Dept. Biochemistry and Molecular Medicine UC Davis, Davis

ThP 430 High-resolution LC-MS for the Characterization of Intact Proteins Using Poly(styrene-co-divinylbenzene) Monolithic Capillary Columns;Sebastiaan Eeltink1; Bert Wouters1; Gert Desmet1; Mario Ursem2; Achim Treumann3; 1Vrije Universiteit Brussel, Brussels, Belgium; 2Dionex Benelux, Amsterdam, The Netherlands; 3NEPAF, North East Proteome Analysis Facility, Newcastle upon Tyne, UK

ThP 431 Development of a Method for Determining Veterinary Drugs used in All Food Producing Animals Using Liquid Chromatography-Mass Spectrometry; Vallerie Muckoya; Kwenga Sichilongo; University of Botswana, Gaborone, Botswana

ThP 432 A Top – Down FT-ICR Precursor Acquisition Independent from Ion Count (PACiFIC) Strategy on an LC Timescale; C. Logan Mackay1; Yihsuan Tsai3;Adam A. Stokes 1; Muhammed Karim1; Matthias Witt 2;David R. Goodlett 3; Pat Langridge Smith1; 1SIRCAMS, Edinburgh, UK; 2Bruker Daltonik GmbH, Bremen, Germany; 3University of Washington, Seattle, WA

ThP 433 Retention Time Shifts Associated with the Variation of Gradient Slope in Peptide RP HPLC: Critical Corrections for Scheduled MRM Analysis; Oleg V. Krokhin; Hoangkim Vu; Alexander Gotfrid; Marine Grigoryan; Vic Spicer; University of Manitoba, Winnipeg, Canada

ThP 434 High Resolution MS for Quantitation of Drugs in Plasma: Evaluation of Resolution and Mass Extraction Window to Achieve Required Selectivity;Yuan-Qing Xia1; Jim Lau2; Bob Walker2; Nga Kit Eliza Fung1; Timothy Olah1; Mohammed Jemal1; 1Bristol-Myers Squibb Company, Princeton, NJ; 2Agilent Technologies, Inc, Wimington, DE

ThP 435 Real Time 2D Separation; Increasing the LC peak Capacity Using Gas Phase Separation Prior to MS Analysis; J.C. Yves Le Blanc1; Emmanuel Varesio2;Gérard Hopfgartner2; 1AB-Sciex, Toronto, Canada; 2School of Pharmaceutical Sciences, EPGL, LSMV, Geneva, Switzerland

ThP 436 A Substructure Approach to Assigning Product Ions from ca 1600 Da Active Pharmaceutical Ingredient;Wei Ding; Yande Huang; Zhenrong Guo; Xujin Lu; Venkatapuram Palaniswamy; Mark S. Bolgar; Bristol-Myers Squibb, New Brunswick, NJ

LC-MS SAMPLE PREPARATION II, 437 - 464 ThP 437 Considerations in Developing a Robust

Ultrafiltration-LC-MS/MS Method for Quantitative Analysis of Unbound Analyte in Human Plasma;Wenkui Li; Hui Lin; Harold T Smith; Francis LS Tse; Novartis Institutes for Biomedical Research, East Hanover, NJ

ThP 438 Plasma Pharmacokinetics of NSC 742410, a Novel Dinucleotide Multidrug, in Mice; Lawrence R. Phillips2; John P. Carter1; Eva Majerova1; Melinda G. Hollingshead2, 2; 1SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD; 2National Cancer Institute-Frederick, Frederick, MD

ThP 439 Coupling One Step Peptide Extraction Using Dihydroxybenzoic Acid to LC/MS for Peptide Sequencing; Elena Romanova; Ji Eun Lee ; Neil L. Kelleher; Jonathan Sweedler; University of Illinois, Urbana, IL

ThP 440 Withdrawn

ThP 441 The Detection of Veterinary Residues in Meat Using on Line SPE in Combination with LC/MS/MS Analysis; Stephen J. Lock1; Liliana Bonetto2; Francisco Mocholí2; 1ABSCIEX, Warrington, UK; 2SAILab, Barcelona, Spain

ThP 442 The First Protein Map of the Spiruline, Arthrospira Platensis Using a Combination of Gel-Based, 2D and 3D-LC MS/MS Approaches; Sabine Matallana-Surget; Baptiste Leroy; Ruddy Wattiez; Dept of Proteomic and Microbiology, UMONS, Mons, Belgium

ThP 443 Determination of Perfluorochemicals in Milk Using Liquid Chromatography - Tandem Mass Spectrometry; Wendy Heiserman; Gregory O. Noonan; Paul South; Timothy Begley; Gregory Diachenko; Food and Drug Administration, College Park, MD

ThP 444 Fully Automated Multiple Reaction Monitoring Quantification of Eicosanoids in Biological Samples by Solid-Phase Extraction–Liquid Chromatography –Tandem Mass Spectrometry; Carlos Ferreiro-Vera2;Jose Maria Mata Granados1; María Dolores Luque de Castro2; Jose Manuel Quesada Gómez3; 1Department of I+D+i, Sanyres Group, Córdoba, Spain; 2University of Cordoba, Cordoba, Spain; 3Reina Sofía Hospital, Córdoba, Spain

ThP 445 Vitamin D and Metabolites: Evaluation of Supported Liquid Extraction (SLE) prior to LC-MS/MS Analysis; Lee Williams; Rhys Jones; Helen Lodder; Geoff Davies; Steve Jordan; Richard Calverley; Claire Desbrow; Gary Dowthwaite; Biotage GB Limited, Cardiff, UK

ThP 446 Identification of Growth Factors in Mouse Plasma by Immunoaffinity Depletion, Low Molecular Weight Protein Enrichment and LC-MS/MS Analysis;Jianying Zhou1; Janani I Shutthanandan1; Brianne O Petritis1; Karl K Weitz1; Ronald J Moore1; David G Camp II1; Rohit N Kulkarni2; Richard D. Smith1; Weijun Qian1; 1Pacific Northwest National Lab, Richland, WA; 2Harvard Medical School, Boston, Massachusetts

ThP 447 Carbamidomethyl-Dithiothreitol Modifications of Cysteine-Containing Peptides: A Potentially Significant Byproduct of Reduction-Alkylation Reactions in Proteomic Studies; Tracy Ystesund1;Wallace Muhonen1; William Old2; Stephane Houel 3;John Shabb1; 1University of North Dakota, Grand Forks, ND; 2University of Colorado, Boulder, CO; 3HowardHughes Medical Instit, Boulder, CO

ThP 448 Construction of Proteomic and Glycomic Enzyme Reactors in Microwave Initiated Monolithic Supports; Yehia Mechref; Yazen Jmeian; Indiana University, Bloomington, IN

ThP 449 New Sample Preparation Method Improves Multi-Residue Screening of Veterinary Drugs in Meat and Milk and Ensures Food Safety; David Jones; Kazuyuki Yamashita; Eugene Chang; Paul Boguzewski; Ritu Arora; Varian Inc., Lake Forest, CA

ThP 450 Strategies for Efficient Sample Preparation of Various Tissues for LC/MS/MS Analysis; Valerie Kempf1; Carly Correll1; Robert Friley1; Larry Goll1;David Singletary1; Thorsten Degenhardt2; 1Enthalpy Analytical, Inc., Durham, NC; 2CempraPharmaceuticals, Inc., Chapel Hill, NC

ThP 451 A Simultaneous Analysis of Multiple Classes of Veterinary Medicine Residues in Whole Blood and Blood Plasma by SPE-LC-MS/MS; Richard Schriner;Jason Clague; Bruce Morris; Hill Laboratories, Hamilton, New Zealand


ThP 452 Development of a Synthetic Protein Quality Control (QC) Standard for the Assessment of Sample Proteolysis Reproducibility; Bryan Krastins1; Amol Prakash2; Scott Peterman3; David Sarracino 1; Michael Athanas4; Taha Rezai1; Mary F Lopez5; 1Thermo Fisher Scientific, Cambridge, MA; 2ThermoFisher Scientific, Cambridge, MA; 3Thermo Electron, Grimes, IA; 4VAST Scientific, Cambridge, MA; 5ThermoFisher, Cambridge, MA

ThP 453 Extension of Species-level Proteome Coverage in a Natural Microbial Community with the GelFree Fractionation System; Brian Dill 1; Nicholas Justice2;Vincent Denef2; Manesh Shah1; Brian Thomas2; Michael Thelen3; Robert Hettich1; Jillian Banfield2; Nathan Verberkmoes 1; 1Oak Ridge National Laboratory, Oak Ridge, TN; 2University of California, Berkeley, Berkeley, CA; 3Lawrence Livermore National Laboratory, Livermore, CA

ThP 454 Characterization of Intact Antibodies by Pre-Fractionation Using Gel Electrophoresis and ESI-MS; James B. Harkins, III; Cindy Brown; Chris Dill; Nghia Chiem; Charles E. Witkowski, II; Jeremy L. Norris; Protein Discovery, Inc., Knoxville, TN

ThP 455 Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric (UPLC-MS/MS) Technique for Quantitation of Protein Free Efavirenz in Human Seminal Plasma; Lindsay B. Avery; Teresa L. Parsons; Meyers David J.; Walter C. Hubbard; Johns Hopkins Hospital, Baltimore, MD

ThP 456 Addressing the Issues of Matrix Resolution and Measurement in Bioanalytical Assays; Geneen Baynham1; Paul Rainville2; Marian Twohig1; Rob Plumb2; Ian Wilson3; 1Waters Corporation, Milford, MA; 2Waters, Milford, MA; 3AstraZeneca, Manchester, UK

ThP 457 Solid Phase Extraction Combined with Liquid Chromatography–Tandem Mass Spectrometry for Determination of Cyromazine and Metabolites in Animal Tissue; Pei-Cheng Wang; Ren-Jye Lee; Pin-RenYu; Maw-Rong Lee; National Chung-Hsing University, Taichung, Taiwan

ThP 458 Improving Internal Standard Reproducibility In LC-MS/MS Analysis of Half-Life Metabolic Stability Samples; Shu Li; Wilson Shou; Cheryl Ferraro; Tatyana Zvyaga; Harold Weller; Bristol-Myers Squibb Company, Wallingford, CT

ThP 459 Rapid and Sensitive LC-MS-MS Method for Dutasteride in Human Plasma; Minjung Chae; Seungwoo Kang; Na Ry Ha; ISS, Seoul, South Korea

ThP 460 Improved Assay Ruggedness Using Hybrid Sample Preparation; Wei Zhou; Yilin Feng; Harold T Smith; Francis Tse; Novartis Institutes for Biomedical Research, East Hanover, NJ

ThP 461 Simulataneous Quantitation of Metformin and Glimepiride in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry; Haejong Jang; International Scitntific Standard, Chuncheon, South Korea

ThP 462 Stability Implications for Quantitative Determination of Bortezomib in Human Plasma Using LC/MS/MS;Linge Li; William Mylott; Bruce Hidy; Rand Jenkins; PPD, Richmond, VA

ThP 463 Investigation of Extraction Recovery for a Novel Anti-cancer Agent in Rat and Beagle Whole Blood;Yifei Liu1; Carl Bates Ii2; Martin Paton3; 1Tandem Labs, a division of Labcorp, West Trenton, NJ; 2Tandem Labs,

West Trenton, NJ; 3Millennium Pharmaceuticals, Inc., Cambridge, MA

ThP 464 Interfacing the Droplet Compartmentalization of High-Efficiency Separations with Mass Spectrometry; J. Scott Edgar; Gloria S. Yen; Robert M. Lorenz; Thomas Schneider; Daniel T. Chiu; University of Washington, Seattle, WA

DRUG METABOLISM: TOXICOLOGY, 465 - 472 ThP 465 Use of HepaRG Cells for Cytotoxicity and

Cytochrome P450 Induction Studies; Yang Song 1, 2;Richard B. Van Breemen1; Scott G Franzblau2;1University of Illinois, Chicago, IL; 2Institute of Tuberculosis Research, Chicago, Illinois

ThP 466 Direct nanoMS Analysis of a Drug-Induced Phospholipidotic Droplet in a Single HepG2 Cell;Kiyoshi Takeshima; Sachiko Date; Hajime Mizuno; Naohiro Tsuyama ; Takanori Harada; Tsutomu Masujima; Hiroshima Univ. BioMed., Hiroshima, Japan

ThP 467 Determination of 13 Quinolones in Bovine Milk by Liquid Chromatography with Tandem Mass Spectrometry; Chae-mi Lim Lim; Hyun-Jeong Kwon; Byung-hoon Cho; Su-Jeong Park; Myeong Ae Kim;Seong-Wan Son; National Veterinary Research & Quarantine Service, Anyang, South Korea

ThP 468 Using Zebrafish to Study the Impact of the Pharmaceuticals Caffeine, Erythromycin, and Fluoxetine Present as Environmental Pollutants to Mammals; Samantha Weber; Rebeca Pinhancos; Molly Gill; Dil Ramanathan; Kean University, Union, NJ

ThP 469 LC-MS/MS Method Development Using Host-Guest Chemistry to Find the Lowest Detectable Limits for Melamine and Cyanuric Acid; Regina Nardi; Karina Nogueir; Dil Ramanathan; Kean University, Union, NJ

ThP 470 Using a Knowledge-Based Software Program to Identify Potential Toxic Intermediates in Drug Compounds; Sian Ives; Ernest Murray; Kristina Fielding; Lhasa Limited, Leeds, UK

ThP 471 LC/MS/MS Method to Identify and Quantify Two Metabolites of Davalintide (AC2307), a Novel Amylin-Mimetic Peptide, in Rat and Dog Plasma;Ying Qu; Chris Bellows; John Ahn; Lala Mamedova; Steven Taylor; Jennifer Burkey; Yan Wang; Amylin Pharmaceuticals, Inc., San Diego, CA

ThP 472 Monitoring Tissue Distributions of Antibody Drug Conjugate Biotherapeutics by LC-MS; Keyang Xu;Luna Liu; Helen Davis; Montserrat Carrasco-Triguero; Sophia Yap; Willy Solis; Michelle McDowell; Rebecca Erickson; Ola Saad; Kelly Flagella; Surinder Kaur; Genentech, Inc., South San Francisco, CA

ENVIRONMENTAL ANALYSIS: HYDROCARBONS & DOM, 473 - 492

ThP 473 Stir Bar Sorptive Extraction (SBSE or Twister™) for Aqueous Environmental Samples; Lorraine Iverson1;St Germain Margie1; Laura Webb1; Ed Pfanncoch2;1USEPA, Kansas City, KS; 2Gerstel, Inc., Linthicum, MD

ThP 474 Analysis of Semi-Volatile Organic Compounds (EPA Method 8270D) in Environmental Water Samples Utilizing Solid Phase Extraction Disks and Carbon Cartridge; David Gallagher; Horizon Technology, Inc, Salem, NH

ThP 475 Recent Developments and in situ Measurements of Underwater Mass Spectrometers; Tim Short; Ryan J. Bell; Strawn K. Toler; Friso H. W. van Amerom; SRI International, St Petersburg, FL

ThP 476 Organic Material Released by Coastal Microbial Consortia upon Incubation with Glucose:


Preliminary Identification of Novel Biomarkers for Bacterial Physiology; Elizabeth Kujawinski; Melissa Soule; Woods Hole Oceanographic Ins, Woods Hole, MA

ThP 477 Optimization of Atmospheric Pressure Photoionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Characterization of Dissolved Organic Matter; David C. Podgorski1;Amy M. Mckenna1, 2; Ryan P. Rodgers1, 2; Alan G. Marshall1, 2; William T. Cooper1; 1Florida State University, Tallahassee, FL; 2National High Magnetic Field Laboratory, Tallahassee, Florida

ThP 478 Detection and Quantification of Low Temperature Air Oxidation of Fluoranthene Reaction Products on Mineral Matrices: Contribution of on-Line GPC-(APCI/APPI)-Q-TOF; Thierry Ghislain; Coralie Biache; Raymond Michles; Pierre Faure; Nancy Université, Vandoeuvre Lès Nancy, France

ThP 479 Evaluation of pH Effects on the Adsorption of N-Nitrosamines by Powdered Activated Carbon Using LC-MS/MS; Yinfa Ma1; Xiaoliang Cheng 1; Honglan Shi2; Craig Adams3; Terry Timmons4; 1Missouri S&T, Rolla, MO; 2Missouri S&T/ERC, Rolla, MO; 3University of Kansas, Lawrence, KS; 4Missouri Department of Natural Resources, Jefferson City, MO

ThP 480 Use of a Model Acid System to Interpret Results from GC/MS Analysis of Environmental Naphthenic Acids; C. Dustin Clark; Nicole Glines; Henry Allred; Charley C. Langley; Utah State University- Uintah Basin, Vernal, UT

ThP 481 Development of Hydrophilic Interaction Chromatographic-Mass Spectrometry Method for the Analysis of the Acid Fraction of Oilsands Processing Water; Brian Fahlman1; Kerry M. Peru 1;John Headley1; Stephen Mcdonald2; 1EnvironmentCanada, Saskatoon, SK; 2Waters Corporation, Beverly, MA

ThP 482 Determination of PAHs in the Oilfield Produced Water at the Nasser Oilfield, Libya by Using Gas Chromatography Mass Spectrometry [GC-MS];Salem Omar; Tom Smith; Malcolm Clench; SheffieldHallam University, Sheffield, UK

ThP 483 Chemical Fingerprinting of Diesel Fuels by Gas Chromatography- and Direct Liquid Introduction-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Vincent Y. Taguchi1; Robert Nieckarz2;Stefan Krolik3; Robert Williams4; 1Ministry of the Environment, Toronto, Canada; 2ETH Zurich, Zurich, Switzerland; 3Consultant to Varian, Montreal, Canada; 4Varian Inc, Walnut Creek, CA

ThP 484 Determination of Levels of Volatile Methyl Silicones in Air Samples from across Canada; Mehran Alaee;Helena Steer; Environment Canada, Burlington, Canada

ThP 485 Selection of an Optimal Reagent Ion for the Chemical Ionization of Atmospherically Relevant Carboxylic Acids; Marc Fiddler1, 2; Solomon Bililign1, 2; 1North Carolina A&T State U., Greensboro, NC; 2NOAA-ISETCSC, Greensboro, NC

ThP 486 Characterization of Airborne Allergens of Crab and Determination of Their Levels in Air Samples of Processing Plants Using Mass Spectrometry; Anas Abdel Rahman1; Andreas Lopata2; Robert Helleur 1;1Memorial university of NL, St. John's, Canada; 2RMITUniversity, Bundoora Campus Melbourne, Australia

ThP 487 Pretreatment Set for the Biological Agnet Analysis;Yong G. Byun; Agency for Defense Development, Daejeon, South Korea

ThP 488 Seasonal, Spatial and Temporal Variations of Air Toxics in the Seattle-Tacoma Airshed Measured by Membrane Introduction Tandem Mass Spectrometry (MIMS-MS/MS); Nicholas G. Davey5, 6; Jacob M. Etzkorn5, 6; Morten Martinsen4, 5; Ji H. Park3; Robert S. Crampton1; Cole T. E. Fitzpatrick1; Timothy V. Larson1,

3; Christopher D. Simpson1; Michael G. Yost1, 2; Erik T. Krogh5, 6; Christopher G. Gill 5, 6; 1DEOHS, University of Washington, Seattle, WA; 2PNASH Center, Seattle, WA; 3Environmental Engineering, Univ. of Washington, Seattle, WA; 4Institutt for kjemi, NTNU, Trondheim, Norway; 5Applied Environmental Research Labs. (AERL), Nanaimo, Canada; 6Vancouver Island University, Nanaimo, Canada

ThP 489 Membrane Inlet- Transportable FT-ICR Mass Spectrometry of Trace Halogenated Compounds in Water; Essyllt Louarn1; Christophe Dehon1; Thibaud Navarro2; Joel Lemaire3; Michel Heninger2; Hélène Mestdagh1; 1Université Paris Sud XI, Orsay, France; 2AlyXan, Orsay, France; 3LCP CNRS - Université Paris Sud 11, Orsay, France

ThP 490 Fast Determination of Synthetic Polycyclic Musks in Solid Samples by Microwave-Assisted Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry; Wang-Hsien Ding; National Central University, Chung-Li, Taiwan

ThP 491 Exploring the Link Between Urban Aerosols and DNA Adducts Using nanoLC-ESI-MS/MS; Joshua J. Klaene1, 2; James Glick1, 2; Euripides G. Stephanou3;Paul Vouros1, 2; 1Department of Chemistry, Northeastern University, Boston, MA; 2Barnett Institute, Northeastern University, Boston, MA; 3Department of Chemistry, University of Crete, Heraklion, Greece

ThP 492 A Mass Spectrometry-Based Approach for Source Identification and Odours Production Remediation;Enrico Davoli; Giancarlo Bianchi; Giorgio Celeste; Marinella Palmiotto; Mario Negri Institute, Milano, Italy

HYDROCARBON & PETRO: BIOFUELS & GEOCHEMISTRY, 493 - 505

ThP 493 A 'Green' Technique for a 'Green' Future: Degraded Biodiesel Sample Analysis Using SFC-UV-MS;Christianne Wicking1; G. John Langley1; Tom Lynch2;1University of Southampton, Southampton, UK; 2BPGlobal Lubricants, Pangbourne, UK

ThP 494 Petroleomic Analysis of Pyrolysis Biomasses Using APPI FT ICR and APPI LTQ-Orbitrap; Erica Smith2;David Perdian2; Christopher Thompson 3; Robert Brown1; Young Jin Lee2; 1Iowa State University, Ames, IA; 2Iowa State University Ames Laboratory US DOE, Ames, IA; 3Bruker Daltonics Inc., Billerica, MA

ThP 495 LC/MS/MS Process Monitoring of Bioethanol Production; Robert Ellis 1; Hubert Piatkowski2; Tom Moy1; Takeo Sakuma1; 1AB SCIEX, Concord, Canada; 2GreenField Ethanol Inc, Chatham, Canada

ThP 496 Pyrolysis and Reactive Pyrolysis GCMS of Biomass, Biofuel Synthesis Perspective; Michael T. Cheng; Chevron Research, Richmond, CA

ThP 497 Hydrocarbon Phenotyping of Algal Species Using Pyrolysis Gas Chromatography Mass Spectrometry;Dinesh Kumar; Tobias Kind; Oliver Fiehn; University of California, Davis, CA

ThP 498 Mass Spectral Characterization of the Pesticide Properties of Bio-oils from Pyrolysis; Luis Caceres1;


Mohammad Hossain1; Christina J. Booker1; Franco Berruti2; Cedric Briens2; Ian M. Scott3; Brian D. McGarvey3; Ken K.-C. Yeung2; Ken Conn3; 1Student, London, Canada; 2Professor/supervisor, London, Ontario; 3Associated Professor/Scientist, London, Ontario

ThP 499 Characterization of Algae Oil: APPI and MS Imaging Approaches Using LTQ-Orbitrap; Erica Smith1, 2; Adam Klein1, 2; Brian Trewyn1, 2; Victor Lin1,

2; Young-Jin Lee1, 2; 1Department of Chemistry, Iowa State University, Ames, IA; 2USDOE- Ames Laboratory, Ames, IA

ThP 500 Characterization of Generation II Biofuels by Ultrahigh-Resolution FT-ICR Mass Spectrometry;Jacqueline M. Jarvis1; Amy M. McKenna2; Rodger Hilten3; K. C. Das3; Ryan P. Rodgers1, 2; Alan G. Marshall1, 2; 1Florida State University, Tallahassee, FL; 2Nat'l High Magnetic Field Lab, Tallahassee, FL; 3University of Georgia, Athens, GA

ThP 501 Beyond Petroleomics-Petroleum Geochemistry for the 21st Century; Thomas Oldenburg; Melisa Brown; Ian Gates; Steve Larter; U of Calgary, Calgary, Canada

ThP 502 Evaluation of APGC TOF MS for Biomarker Analysis in Petroleum Geochemistry; Hilary J. Major1;Keith Hall2; Andrew Gize3; Gareth Harriman4; Tony Newton1; 1Waters Corporation, Manchester, UK; 2HallAnalytical, Manchester, UK; 3The University of Manchester, Manchester, UK; 4GHGeochemicalServices, Bebbington, UK

ThP 503 Molar Weight Distribution of Elements in Crude Oil and Vacuum Residue by Microgel Permeation Chromatography Coupled to High Resolution ICPMS; Justyna Dural1; Pawel Pohl1; Hervé Carrier4;Isabelle Merdrignac3; Charles Philippe Lienemann3;Bruno Grassl2; Brice Bouyssiere1; Ryszard Lobinski1;1LCABIE - CNRS UMR 5254, Pau, France; 2EPCP - IPREM - CNRS UMR 5254, Pau, France; 3IFP, Cedi « René Navarre », Vernaison, France; 4LFC, UMR 5150, UPPA, Pau, France

ThP 504 FT-ICR/MS Molecular Analysis of Vanadium and Nickel Containing Compounds in Heavy Crude Oil;Jeremie Ponthus; Isabelle Merdrignac; Frederique Perbost-Prigent; Institut Francais du Petrole, Solaize, France

ThP 505 Detailed Characterization of Metalloporphyrins in Petroleum by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Kathleen Edwards;Kuangnan Qian; Anthony Mennito; Clifford C. Walters; ExxonMobil Research & Engineering, Annandale, NJ

FORENSICS, 506 - 528 ThP 506 Development of Forensic Applications of Planar

Differential Ion Mobility Spectrometry (DMS) Interfaced to an Ion Trap Mass Spectrometer; Adam B Hall1, 2; Paul Vouros1; James Glick1; Samantha Mosley1; Erkinjon Nazarov3; Stephen L Coy3;1Northeastern University, Boston, MA; 2Boston University: Biomedical Forensic Sciences, Boston, MA; 3Sionex Corp., Bedford, MA

ThP 507 Evaluation of Performance and Benefit of Ultrahigh-Resolution ESI-TOFMS Coupled to Fast Chromatography in the Application of Forensic Screening; Katherine Kellersberger1; Anna Pelander2;Petra Decker3; Carsten Baessmann 3; 1Bruker Daltonics, Billerica, MA; 2University of Helsinki, Helsinki, Finland; 3Bruker Daltonik GmbH, Bremen, Germany

ThP 508 Forensic Science Applications with Nanomanipulation-Nanospray Ionization: Analysis of Paint, Drugs, and Ink Samples; Nicole Wallace1, 2;Ubisha Joshi1, 2; Guido F. Verbeck1, 2; 1, Denton, TX; 2University of North Texas, Denton, Texas

ThP 509 Quantitative Analysis of Organic Gunshot Residues by LC/MS/MS; Yanan Yang1; Na Pi1; Elisabeth Reeves2; Maryann Shen1; Faye Springer2; 1Agilent Technologies, Inc, Santa Clara, CA; 2SacramentoCounty District Attorney Crime Lab, Davis, CA

ThP 510 A Proteomic Approach to Body Fluid Identification for Forensic Applications; Heyi Yang; Bo Zhou; Donald Siegel; Yingying Tang; Mechthild Prinz; Office of Chief Medical Examiner, New York, NY

ThP 511 LSD and 9,10-Dihydro-LSD Analysis in Street Drug Blotter Samples (Rio de Janeiro, Brazil) via EASI-MS; Wanderson Romão1; Bruno D. Sabino2; Amadeu C. Júnior2; Boniek G. Vaz1; Deleon N. Correa3; Marcos N. Eberlin1; 1Universidade Estadual de Campinas (Unicamp), Campinas, Brazil; 2Instituto de Criminalística Carlos Éboli, Rio de Janeiro, Brazil; 3Technical-Scientific Police Superintendence, São Paulo, Brazil

ThP 512 Fast SFC/MS/MS Analysis of Metabolism of Chiral Drugs of Abuse; Maggie Lee1; D.J. Tognarelli2; Carmai Seto3; Tanya Gamble3; Robert Ellis 3; Tom Moy3; Takeo Sakuma3; 1Centre for Addiction and Mental Health, Toronto, ON; 2JASCO, Easton, MD; 3AB SCIEX, Concord, Canada

ThP 513 Rapid Analysis of Phenethylamines in Biological Fluids by Ambient Sampling Dielectric Barrier Discharge Mass Spectrometry (DBD/MS); Hiroko Furuya1; Lee Chuin Chen2; Yutaka Hashimoto2; Kenichi Takekawa1; Kenzo Hiraoka2; 1Forensic Science Laboratory, Fuefuki, Japan; 2University of Yamanashi, Kofu, Japan

ThP 514 Measurement of Total Drug Consumption in a Small Population by Tandem Mass Spectrometry; M. Paul Chiarelli1; Deepika Panawennage1; Sara Castiglioni2;Ettore Zuccato2; Enrico Davoli2; Qian Wang1; Timothy O'Brien1; 1Loyola University, Chicago, IL; 2Mario Negri Institute, Milano, Italy; 3Mario Negri Institute, Milano, Italy

ThP 515 Quantitation of Salvinorin A and Metabolite in Human Blood, Plasma and Urine by LC/MS/MS;Allan Xu; Barry Logan ; Matthew McMullin; NMS, Willow Grove, PA

ThP 516 New Strategy for Quantification of THC Carboxylic Acid Direct from Hair; Detlef Thieme2; Hans Sachs3;Axel Besa1; Birgit Schlutt1; Detlev Schleuder1; 1AB SCIEX, Darmstadt, Germany; 2Department of Sports Medicine and Doping Analysis, Kreischa, Germany; 3FTC Munich, Munich, Germany

ThP 517 A Validated Method for Determination of THC-COOH in Hair Using Large-Volume Injection and GC/NCI-MS with High Mass Enhancement; Yuan-Jhe Chang; Yan Zin Chang, Taichung, Taiwan

ThP 518 Simultaneous Determination Drugs of Abuse in Hair Using LC-MS/MS with API-Active Derivatization;Pin-Duo Lee; Taichung, Taiwan

ThP 519 Comprehensive Screening of Aconitum Alkaloids in Kampo Herbal Medicine, in Human Serum and Urine by High Resolution LC/TOF Mass Spectrometry; Masahiko Takino1; Makiko Hayashida2;Youkichi Ohno2; 1Agilent Technologies, Hachioji-Shi, Japan; 2Nippon Medical School, Tokyo, Japan


ThP 520 Determination of Clenbuterol in Dietary Supplements; John C Travis; David L. Weller; Kurtis R Kneen; Kerri L LeVanseler; Scott K Clipper; NSF International, Ann Arbor, MI

ThP 521 Simultaneous Separation and Confirmation of 10 Opiates in Equine Plasma by Capillary-Electrophoresis-Tandem Mass Spectrometry;Xiaoqing Li1; Cornelius Uboh 2; Lawrence Soma1; Fuyu Guan1; Youwen You1; Mark Kahler2; Jeffrey Rudy2;Ying Liu1; Jiwen Chen1; 1UPENN, West Chester, PA; 2West Chester University, West Chester, PA

ThP 522 LC-MS/MS Analysis of Corticosteroids in Equine Urine; Yan Chang; Don Catlin; Anti-Doping Research, Inc., Los Angeles, CA

ThP 523 High-Throughput Analysis of 60 Anabolic and Androgenic Steroids in Equine Plasma by Liquid Chromatography-Tandem Mass Spectrometry with Library Searching; Ying Liu1; Cornelius Uboh 2;Lawrence Soma1; Xiaoqing Li1; Fuyu Guan1; Youwen You1; Jeffrey Rudy2; Jinwen Chen1; 1UPENN, West Chester, PA; 2PA Equine Toxicology and Research Center, West Chester, PA

ThP 524 Anti-Doping Control Using Comprehensive Multidimensional Gas Chromatography Time-of-Flight Mass Spectrometry (GCxGC-TOFMS) for Enhanced Detection of Anabolic Steroids in Urine;John R. Heim; Doug Staples; Joe Binkley; LECO Corporation, St. Joseph, MI

ThP 525 Fast Screening and Identification of WADA Prohibited Anabolic Steroids in Urine by UHPLC/MS/MS; Jerry Zweigenbaum; Agilent Technologies, Wilmington, DE

ThP 526 Rapid Detection and Confirmation of -Agonist Drug Residues in Meat Samples by Liquid Chromatography-SRM and Mass Spectral Library Searching; Anna M. Przyborowska; Dorentina Bexheti; John M. Halket; King's College London, London, UK

ThP 527 Confirmatory Analysis of Recombinant Human Erythropoietin and Analog in Equine Plasma by LC-MS; Fuyu Guan1; Cornelius Uboh2; Lawrence Soma1;Jinwen Chen1; 1University of Pennsylvania, Kennett Square, PA; 2PA Equine Toxicology and Research Center, West Chester, PA

ThP 528 Characterization of Phospholipids-Based Drugs and Their Effect on Phospholipids Profiles in Biological Fluids Assessed by HPLC-ESI-MS/MS: Diagnostic and Forensic Implications; Simone Esposito2; Xavier de la Torre2; Monica Mazzarino2; Francesco Botre1, 2;1Sapienza University of Rome, Rome, Italy; 2LaboratorioAntidoping FMSI, Rome, ItalyCOMPUTER APPLICATIONS, 529 - 546

ThP 529 Molecular Dynamics Simulations of Matrix Assisted Laser Desorption Ionization: Analysis of Intermolecular Matrix-Analyte Interactions; Shivangi Nangia1; Barbara Garrison2; 1Pennsylvania State University, University Park, PA; 2Penn State University, University Park, PA

ThP 530 Numerical Simulation of Ion Dynamics in Collision Multipole Ion Guides; Victor Laiko1; Craig M. Whitehouse2; 1Perkin Elmer, Inc., Branford, CT; 2PerkinElmer, Branford, CT

ThP 531 New Possibilities of Capacity Method Improvement for Ion Clouds Dynamics Simulations in Traps with Arbitrary Geometry Electrodes; Pavel Ryumin; Ivan Boldin ; Eugene Nikolaev; The Institute for Energy

Problems of Chemical Phys, Moscow, Russian Federation

ThP 532 Accelerated Trajectory Simulations for SIMION with a Beowulf Cluster; Peter Williams; Agilent Laboratories, Santa Clara, CA

ThP 533 Internet Telepresence for Cyber Mass Spectrometry;Kermit K. Murray1; Damien A. Narcisse2; 1LouisianaState Univ., Baton Rouge, LA; 2Louisiana State Universit, Baton Rouge, LA

ThP 534 Achieving Regulatory Compliant Biotherapeutic LC/QTof-MS analyses within a Prototype Data Acquisition, Processing, and Bioinformatics Environment; Scott Berger; Steven Bird; Virginia Corbin; Waters Corporation, Milford, MA

ThP 535 Aquaphile: Database for Managing Labeled Peptides and MRM / SRM Assays; Simon Letarte; Andrew Keller; Leroy Hood; David Galas; Institute for Systems Biology, Seattle, WA

ThP 536 A Unified Algorithm for Deconvoluting Electrospray Ionization Mass Spectral Data; John Skilling1; Keith G Richardson2; Jeff Brown 2; Iain D G Campuzano2; Brian N Green2; Jason L Wildgoose2; 1Maximum Entropy Data Consultants Ltd., Kenmare, Ireland; 2Waters Corporation, Manchester, UK

ThP 537 A Software Package to Facilitate Analysis of Intrinsic Fluorescence and Tandem Mass Spectrometry Data Sets for Quantitative Proteomics; Mark A. Tervo;Jason D. Russell; Craig D. Wenger; Joshua J. Coon; University of Wisconsin, Madison, WI

ThP 538 Enhanced Sequencing Accuracy Using a Method Combining de novo Sequencing and tag Search Results for Tandem Mass Spectral Data; Jingwen Yao1; Ikuo Konishi2; 1Shimadzu Research Laboratory (Europe) Ltd, Manchester, UK; 2Shimadzu Corporation, Kyoto, Japan

ThP 539 Advantages of Proteomic Analyses Using a Hybrid Q-TOF Instrument with MS^E Capability; Adrian R Woolfitt; Hercules Moura; Yulanda M. Williamson; Rebecca R Terilli; Maria I Solano; John R. Barr; Centers for Disease Control and Prevention, Atlanta, GA

ThP 540 Y-Branch Algorithm for Filtering MS/MS Datasets Obtained by Higher-Energy C-Trap Dissociation Dramatically Increases Protein Identification Efficiency; Yaroslav Lyutvinskiy; Roman Zubarev; Karolinska Institute, Stockholm, Sweden

ThP 541 Cross Re-Integration over Sample Set for Better Accuracy in Metabolite Analysis; Hongping Dai;Corey D. DeHaven; Anne Evans; Metabolon, Inc., Durham, NC

ThP 542 Standardizing Metabolomic MS Databases: An Open-Source Tool to Cross-Reference Chemicals by Structure Codes, Compound Names and Database Identifiers; Gert Wohlgemuth; Pradeep Haldiya; Oliver Fiehn; UC Davis, Davis, CA

ThP 543 Mass Spectrometry and Component Data Analysis (CODA) Processing in Pharmaceutical HPLC Method Development; Guilong (Charles) Cheng; Pfizer, Inc., Groton, CT

ThP 544 “PetroMassSpec: Simplifying Petroleomic Data Analysis via Software Processing and Graphics”;Yuri E. Corilo1; Boniek G. Vaz1; Rosineide C. Simas1;Clécio F. Klitzke1; Heliara Lopes Nascimento1; Rosana C. L. Pereira2; Wagner L. Bastos2; Marcos N. Eberlin1;

1ThoMSon Mass Spectrometry Laboratory - UNICAMP, Campinas, Brazil; 2PETROBRAS, Rio De Janeiro, Brazil


ThP 545 Creation and Utility of a Large Mass Spectral Library in Hardware Independent Format; Graham A. McGibbon1; Andrey Paramonov2; Vitaly Lashin2;Graeme Whitley4; Bernd U. Berger; 1ACD/Labs,Toronto, Canada; 2ACD/Labs (Ltd), Moscow, Russia; 3John Wiley & Sons, Inc., Weinheim, Germany; 4Wiley-Blackwell, Hoboken, NJ

ThP 546 Parameterless Peak Detection (PPD): Software for Fully Automatic Interpretation of Chromatographic Profiles; David Wright; Rob Grothe; Thermo Fisher Scientific, San Jose, CA

HIGH THROUGHPUT ANALYSIS/ROBOTICS, 547 - 576 ThP 547 Open Access UPLC/MS for the Analysis of Synthetic

Peptides and Recombinant Proteins; Bethanne Warrack1; Brian Redding2; Guodong Chen1; David Wang-Iverson1; 1Bristol-Myers Squibb, Princeton, NJ; 2Spectrix Analytical Services, Princeton, NJ

ThP 548 A Fully Automated Workflow for High-Throughput Serum Peptide- and Protein Profiling; Marco R. Bladergroen; Yuri E.M. Van Der Burgt; Hans Dalebout; Magnus Palmblad; André M. Deelder; Leiden University Medical Center, Leiden, Netherlands

ThP 549 High Throughput Mass Spectrometry Based Screening for Inhibitors of Amyloid Beta Protein Oligomerization; Kevin Krock; Richard B. Van Breemen; University of Illinois at Chicago, Chicago, IL

ThP 550 Development of High Throughput Screening Pulsed Ultrafiltration Mass Spectrometry (HTS-PUF MS);Jerry White1; Jeff Dahl1; Richard B. Van Breemen2;1University of Illinois at Chicago, Chicago, IL; 2University of Illinois, Chicago, IL

ThP 551 Analyzing Peptide Arrays by SAMDI MS/MS;Stephanie Boussert2; Zachary A. Gurard-Levin1; Milan Mrksich2; 1University of Chicago, Chicago, IL; 2University of Chicago, HHMI, Chicago, IL

ThP 552 The Next Generation SAMDI-TOF MS Platform for High-Throughput Label-free Detection of Intact Proteins; Jaekuk Kim; Steven Patrie; UT Southwestern Medical Center, Dallas, TX

ThP 553 A High-Throughput MALDI MS Assay for Multiplexed Read-Out of Histone Demethylase Inhibition; Hamzah N Freeman1; Melanie Leveridge1;Sue Hutchinson1; Andy West1; Anja Resemann2; Detlev Suckau2; Klaus Schneider1; 1GlaxoSmithKline, Essex, UK; 2Bruker Daltonics, Bremen, Germany

ThP 554 In-depth Analysis of Membrane Proteome by Combined Approaches of GELFrEE, FASP, and Microwave Digestion; Yanbao Yu; Ling Xie; Xian Chen; University of North Carolina, Chapel Hill, NC

ThP 555 Fully Automated Preparation, Extraction, and Subsequent LC/MS/MS Detection of a Panel of Ceramides as potential Mechanistic Biomarkers;Steve Gernhardt; Justin Walton; Zhenhua Gu; Parya Nouri; Joseph Tweed; Rick Steenwyk; Pfizer, Clinton, CT

ThP 556 High Throughput Native Glycan Profiling by MALDI-MS and Chip-based LC ESI-MS; Heidi Zhang1; Dayin Lin2; Christian Graf1; Lukas Trojer2;1Novartis Biologics, Basel, Switzerland; 2Agilent Technologies, Waldbronn, Germany

ThP 557 High-Throughput Quantitative Analysis of Immunosuppressants Using an Integrated Multiplex 2D-LC MS-MS System; Min J. Yang; Peter Kovarik; Adrian Taylor; John Gibbons; Tom Covey; AB SCIEX, Concord, Canada

ThP 558 Evaluation of a New Peak Integration Algorithm for High Throughput LC/MS/MS Data Processing; John Gibbons1; Rick King2; Lyle Burton1; Gordana Ivosev1;Adam Lau1; Feng Zhong1; Hesham Ghobarah1; 1AB Sciex, Concord, Ontario, Canada; 2PharmacadenceAnalytical Services, Hatfield, Pennsylvania

ThP 559 Flow Injection Electrospray Ionization Mass Spectrometry (FI-ESI-MS) Gradient Ratio Standard Addition (GR-SA) for Non-Chromatographic Quantification of Pharmaceutical Active Ingredients;Dana Hostetler; Facundo Fernandez; Georgia Institute of Technology, Atlanta, GA

ThP 560 Advantages of Multiple HPLC Systems and a Single Mass Spectrometer Data File for High Throughput Screening; Matthew Berube; Keeley Murphy ; Francois A. Espourteille; Thermo Fisher Scientific, Franklin, MA

ThP 561 Laser Diode Thermal Desorption (LDTD) -MS/MS Analysis of Chloroquine, Proguanil and Mefloquine in Human Urine; Sherry Liu1; Scott Reuschel1; Min Meng 1; Patrick Bennett1; Serge Auger2; Pierre Picard2;Patrice Tremblay2; 1Tandem Labs, Salt Lake City, UT; 2Phytronix Technologies, Inc., Quebec, QC

ThP 562 Challenges and Opportunities in Adapting LC/MS/MS to High-Throughput Screening; John M. Peltier; Nicole White; David Farley; Novartis Institutes for BioMedical Research, Cambridge, MA

ThP 563 A Novel Algorithm for Automated High-Throughput Quantitative MS/MS Optimization Incorporating Saturation Control; Anthony Romanelli1; Kevin Shirey2; John Janiszewski3; 1AB SCIEX, Framingham, MA; 2Sound Analytics, Niantic, CT; 3Pfizer, Groton, CT

ThP 564 Enhanced Workflow for Rapid Identification of Ultrafiltration Screening Ligands; Jeff Dahl1; Shunyan Mo1; W. Jeffrey Hurst2; Richard B. Van Breemen1;1University of Illinois, Chicago, IL; 2The Hershey Company, Hershey, PA

ThP 565 Application of Robotic Sample Preparation Program (RSPP) to Sample Dilutions for the Determination of BMS-562086 in Human Plasma by LC-MS/MS; Hao Jiang; Zheng Ouyang; Naiyu Zheng; Jianing Zeng; Bristol-Myers Squibb, Princeton, NJ

ThP 566 Integration and Streamlining of Robotics, Automated Tuning LC/MS/MS, And LIMS Workflows For High Throughput In Vitro ADME Bioanalysis; John Laycock; Amgen, Inc, Thousand Oaks, CA

ThP 567 LC/MS of Compound DMSO Solutions Acoustically Dispensed in Nanoliter Volumes; Mark J. Hayward;Paul M. McCoy; Kristine M. Maurer; Bodil Ibsen Marstrand; Emily J Reinhard; Qing Ping Han; LundbeckResearch USA, Stockton, NJ

ThP 568 Rapid Screening of Degradation Products from Lignocellulosic Biomass Processing Using LC/MS/MS on a Hybrid Triple Quadrupole Linear Ion Trap;Ramin Vismeh; Shishir Chundawat ; James Humpula ; Balan Venkatesh; Bruce E. Dale; A. Daniel Jones; Michigan State University, East Lansing, MI

ThP 569 A Fully Automated Online Extraction Method for Determination of Raltegravir in Plasma by LC/MS/MS; Yifan Shi; Min Meng ; Lisa Rohde; Troy Voelker; Lin Tan; Scott Reuschel; Patrick Bennett; Kc Van Horne; Tandem Labs, Salt Lake City, UT

ThP 570 Towards Automated Evaluation of Result Accuracy for LC/MS/UV/ELSD/CLND Substance Screening – Supporting Library Management and Medicinal Chemistry; Mark Bayliss; Joseph Simpkins; Virscidian Inc., Raleigh, NC


ThP 571 Quantitation of Plasma Endocannabinoids by UFLC-MS/MS; Biochemical Fate of 2-Arachidonylglycerol;Matthew Blatnik; Jason Barricklow; Yizhong Zhang; Pfizer Inc., Groton, CT

ThP 572 Development of a High-Throughput MS-Based Bioanalytical Method for Assessment of P-Glycoprotein Inhibition; Andrew Wagner1, 2; Janet Kolb1, 2; John Herbst1, 2; Tatyana Zvyaga1, 2; Conway Charlie1, 2; Harold Weller1, 2; Wilson Shou 1, 2; Can "Jon" Ozbal3; 1Bristol-Myers Squibb Company, Wallingford, CT; 2Bristol-Myers Squibb Company, Princeton, NJ; 3BIOCIUS Life Sciences, inc, Woburn, MA

ThP 573 The Approach to Improve Recovery in Caco-2 Permeability Suite Supported by LC-MS/MS;Xianmei Cai; Aaron Walker; Charles Cheng; Anthony Paiva; Ying Li; Janet Kolb; John Herbst; Juan Cadavid; Wilson Shou; Bristol-Myers Squibb Company, Wallingford, CT

ThP 574 Two-Dimensional Preparative SFC/MS System for Combined Achiral/Chiral Separations; Yinong Zhang;John Eve; Rongda Xu; Lu Zeng; Daniel B. Kassel; Takeda San Diego, Inc., San Diego, CA

ThP 575 Detection of Active Ingredients in Pharmaceutical and Quasi Drugs with DART Ion Source and TOF-MS; Haruo Hosoda1; Jouji Seta1; Jun Watanabe1;Takashi Nirasawa1; Noriyuki Iwasaki1; Yoshiyuki Takahashi2; Teruhisa Shiota2; 1bruker Daltonics KK, Yokohama, Japan; 2AMR, Inc., Tokyo, Japan

ThP 576 Solvent/Sample Interaction in the GC/MS Analysis of Amines; O. David Sparkman; Matthew Curtis; Patrick R. Jones; University of the Pacific, Antioch, CA

IMAGING MS: SMALL MOLECULES, 577 - 599 ThP 577 Quantitative Imaging of Cocaine and Its Metabolites

in Brain Tissue Using MALDI-MS/MS with a Multi-Notch SWIFT Waveform; Richard F. Reich; Richard A. Yost; Department of Chemistry, University of Florida, Gainesville, Florida

ThP 578 Quantitative MALDI Imaging: Glucosinolates on Leaf Surfaces; Ales Svatos1; Rohit Shroff1, 2; 1Max Planck Institute for Chemical Ecology, Jena, Germany; 2ETH Institute of Molecular Systems Biology, Zurich, Switzerland

ThP 579 Fine mapping the Spatial Distribution and Concentration of Unlabeled Drugs within Tissue Micro-Compartments Using Imaging Mass Spectrometry; Anna Nilsson1; Thomas Fehniger2; Lena Gustavsson2; György Marko-Varga2; Per E. Andren1;1Uppsala University, Uppsala, Sweden; 2AstraZeneca R&D, Lund, Sweden

ThP 580 Microscopic Imaging Mass Spectrometry on Hepatic Micrometastasis of Human Colon Cancer Xenografts in Superimmunodeficient NOG Mice; Akiko Kubo1;Mitsuyo Ohmura1; Masatoshi Wakui1; Kenji Kawai2;Chiyoko Nishime2; Takako Hishiki1; Mitsuru Murata1;Kiyoshi Ogawa3; Mitsutoshi Setou4; Makoto Suematsu1;1Keio University, Tokyo, Japan; 2Central Institute for Experimental Animals, Kanagawa, Japan; 3Shimadzu Corporation, Kyoto, Japan; 4Hamamatsu University School of Medicine, Okazaki, Japan

ThP 581 MALDI Imaging of Retinoids in RPE-Choroid Tissue; Kevin L. Schey 1; Rosalie Crouch2; Anne Hanneken3; Patrice Goletz2; Lorie Blakeley2; Yiannis Koutalos2; Angus Grey1; Zsolt Ablonczy2; 1Vanderbilt University, Nashville, TN; 2Medical University of South Carolina, Charleston, SC; 3The Scripps Research Institute, La Jolla, CA

ThP 582 Capturing Bacterial Metabolic Exchange Using DESI-Imaging Mass Spectrometry; Jeramie Watrous1;Pieter Dorrestein 2; 1University of California, San Diego, San Diego, CA; 2University of California, San Diego, Skaggs School, La Jolla, CA

ThP 583 Mapping the Effect of Chemotherapy on Positron Emission Tomography (PET) Tracer Uptake in Tumour Micro-Environments Using Imaging Mass Spectrometry; Emrys A Jones; Nicholas Lockyer; Adam McMahon; Kaye Williams; University of Manchester, Manchester, UK

ThP 584 Utilizing Complimentary in-situ Imaging Platforms to Understand Tumor Penetration for Oncology Drug Discovery; Stacey R. Oppenheimer; David C. Gale; Dean Wilkie; Leslie A. Obert; Pfizer Inc., Groton, CT

ThP 585 The Application of Mass Spectrometry Imaging to Determine Biodistribution of a Small Molecular Agent, RTA 769, in Rat Brain Tissue; Jihai Pang;Rhea Robyn; Edd Filex; Quanyuan Xu; Mary Johansen; Tim Madden; UT, M.D. Anderson Cancer Center, Houston, TX

ThP 586 Exploration of the Metabolome from Medicago Truncatula roots and Nodules by MALDI Mass Spectral Imaging; Hui Ye1; Ruibing Chen1; Maegen Howes-Podoll2; Jean-Michel Ané2; Lingjun Li1; 1Schoolof Pharmacy, UW-Madison, Madison, WI; 2Departmentof Agronomy, UW-Madison, Madison, WI

ThP 587 Profiling of Polyamine Distribution in the Mouse Embryo Using MALDI-Q-IM-TOF MS; Han-Jia Lin2;Chia-Wei Wen1, 2; Yet-Ran Chen1; 1Agricultural Biotechnology Research Ceneter, Taipei, Taiwan; 2Taiwan Ocean University, Keelung, Taiwan

ThP 588 Successful Application for Distribution Image of Chloroquine in Ocular Tissue of Pigmented Rat Using MALDI-Imaging Quadrupole Time-of-Flight Mass Spectrometry; Yasuhiro Yamada3; Hidefumi Kaji3; Henry Y. Shion 1; Motoji Oshikata2; Yukari haramaki2; Alan L Millar1; John P. Shockcor1; 1Waters Corp., Milford, MA; 2Nihon Waters, Tokyo, Japan; 3Mitsubishi Tanabe Pharmaceuticals Corporation, Saitama, Japan

ThP 589 SIMS and MALDI Mass Spectrometry Imaging of Drugs and Metabolites in Whole-Body Mouse Sections; Erika Amstalden Van Hove1; Donald Smith1;Lieve Dillen2; Filip Cuyckens2; Katelijne Anciaux2; Ron M.A. Heeren 1; 1FOM Institute AMOLF, Amsterdam, Netherlands; 2Johnson & Johnson PRD, Beerse, Belgium

ThP 590 Visualization of Spatiotemporal Metabolic Behaviors by in situ Metabolomic Imaging with MALDI-MS;Daisuke Miura; Yoshinori Fujimura; Mayumi Yamato; Fuminori Hyodo; Hirofumi Tachibana; Hiroyuki Wariishi; Kyushu University, Fukuoka, Japan

ThP 591 Direct Observation of Drug Delivery Using MALDI FT-ICR MS Imaging Method; Kyu Hwan Park1; Ji-Yeon Suh1, 2; Hyo-Jik Yang1; Jeong Kon Kim2;Gyunggoo Cho1; Hyun Sik Kim1; 1Korea Basic Science Institute, Ochang-Myun Cheongwon-Gun, South Korea; 2Asan Medical Center, University of Ulsan, Seoul, South Korea

ThP 592 Fast Determination of Drugs of Abuse in Human Tissue Sections by MALDI Mass Spectrometric Imaging; Tiffany Porta1; Thomas Kraemer2; Emmanuel Varesio1; Gérard Hopfgartner1; 1School of Pharmaceutical Sciences, EPGL, LSMS, Geneva,


Switzerland; 2Institute of Legal Medicine, University of Zurich, Zurich, Switzerland

ThP 593 Imaging of Toxic Small Molecules in Potato Using Matrix-Assisted Laser Desorption/ionization Mass Spectrometric Imaging; Miyoung Ha1, 2; Hyesun Maeng1, 2; Jong Hwan Kwak3; Yangsun Kim1, 2; OkPyo Zee3; 1Hudson Surface Technology, Inc., Newark, DE; 2Applied Surface Technologly, Inc.,, Suwon, South Korea; 3Sungkyunkwan University, Suwon, South Korea

ThP 594 Affects of Anesthesia on Rat Tail Cross-Sections Imaged by TOF-SIMS; Itsuko Ishizaki1; Yoshiharu Ohasi1; John Hammond3; Scott Bryan3; Akiharu Kubo2;1ULVAC-PHI, Chigasaki, Japan; 2Keio University, Tokyo, Japan; 3Physical Electronics, Chanhassen, MN

ThP 595 LDI-MS Imaging of Flavonoid Metabolites in Genetically Mutated Arabidopsis; Andrew Korte1, 2;Young-Jin Lee1, 2; Zhihong Song1, 2; Edward Yeung2;Basil Nikolau1; 1Iowa State University, Ames, IA; 2AmesLaboratory, US Department of Energy, Ames, Iowa

ThP 596 Identification and Distribution of a Reserpine Metabolite in Dosed Whole-Body Rat Tissue Using MS and MS/MS Mass Spectrometric Imaging; John McNamara1; Patrick Pribil1; Sheerin Shahidi-Latham2;1AB SCIEX, Concord, ON; 2Genentech Inc, San Francisco, CA

ThP 597 Integrating Quantitation and Multiple Imaging Modalities for Mass Spectrometry Imaging Applications in Drug Discovery; Stormy Koeniger1;Nari Talaty1; Yanping Luo1; Jane Fagerland2; Damien Ready1; Margery Stark Altman2; Xiaoming Hu1; Martin Voorbach1; Terese Seifert1; Steven Cepa1; Jennifer Bouska3; Robert W Johnson1; Stephen Spanton1;1Advanced Technology, GPRD, Abbott Laboratories, Abbott Park, IL; 2Preclinical Safety, GPRD, Abbott Laboratories, Abbott Park, IL; 3Cancer Reasearch, GPRD, Abbott Laboratories, Abbott Park, IL

ThP 598 MALDI-MS Imaging of Small Molecule Drugs in Animal Eye, Brain, Lung, Liver and Tumor;Fangbiao Li; Yunsheng Hsieh; Walter Korfmacher; Merck Research Laboratories, Kenilworth, NJ

ThP 599 MALDI MS Imaging and LDI MS Imaging of Analyte Distribution in Mammalian Peripheral Sensory-Motor Circuit; Stanislav Rubakhin1; Jonathan Sweedler2; 1Beckman Institute, UIUC, Urbana, IL; 2University of Illinois, Urbana, ILION MOLECULE REACTIONS, 600 - 622

ThP 600 Adsorption of Aldehydes on Trapped Biomolecular Ions; Xiangguo Shi1; Jianhua Ren 2; Ryan M. Danell3;Joel H. Parks1; 1Harvard University, Cambridge, MA; 2University of the Pacific, Stockton, CA; 3DanellConsulting, Greenville, NC

ThP 601 Ion-Molecule Reactions for Detecting the Low Molecular Weight Toxic Industrial Compound Ammonia Using Miniature Cylindrical Ion Trap Mass Spectrometers; Jonell Smith; Adam Keil; Robert J. Noll; R. Graham Cooks; Purdue University, West Lafayette, IN

ThP 602 Effects of Inherent Receptor Chirality on the Recognition of Aminosugars; Matthias C. Letzel;Caterina Fraschetti; Marlene Paletta; Jochen Mattay; University of Bielefeld, Bielefeld, Germany

ThP 603 Production, Isolation and Reactivity Studies of Metal(IV)-Oxo Species with Biomimetic Ligands: A Tandem Mass Spectroscopic and Density Functional Theory Study; Ivan F Taylor1; Stephen J Blanksby2;Stephen B Colbran1; Gary D Willett1; 1University of New

South Wales, Sydney, Australia; 2University of Wollongong, Wollongong, Australia

ThP 604 Gas-phase Reactivity of the 4,5-Didehydroquinolium and 4,5-Didehydroisoquinolinium Biradicals Toward Amino Acids and Dipeptides; Enada F Archibold1;Mingkun Fu 2; Nelson Vinueza1; George Pates1; John Nash1; Hilkka Kenttamaa1; 1Purdue University, West Lafayette, IN; 2The Takeda Oncology Company, Cambridge, MA

ThP 605 A Study on the Generation of a Pyridine-Based Tetraradical by SORI-CAD; Jennifer Reece1;Bart omiej Jankiewicz2; Vanessa Gallardo1; John Nash1;Hilkka Kenttamaa1; 1Purdue University, West Lafayette, IN; 2Military University of Technology, Warsaw, Poland

ThP 606 Kinetic Reactivity of an Aromatic , , -Triradical: the 2,3,5-Tridehydropyridinium Cation; Mohammad Sabir Aqueel1; Nelson Vinueza1; Bart omiejJankiewicz2; John Nash1; Hilkka Kenttamaa1; 1Purdue University, West Lafayette, IN; 2Military University of Technology, Warsaw, Poland

ThP 607 A Gas-Phase Study on Two para-Benzynes in an FT-ICR Mass Spectrometer; Lindsey M. Kirkpatrick1;Bart omiej J. Jankiewicz2; Nelson R. Vinueza1; John J. Nash1; Hilkka Kenttamaa1; 1Purdue University, Department of Chemistry, West Lafayette, IN; 2Military University of Technology, Warsaw, Poland

ThP 608 Substituent Effects on the Reactivity of 3-X-2,4,6-Tridehydropyridinium and 3-X-2,4-Didehydropyridinium Ions (X = Substituent); Jinshan Gao; Anyin Li; John Nash; Hilkka Kenttamaa; Chemistry Department, West Lafayette, IN

ThP 609 Measurement of the Proton Affinities of Dehydro- and Didehydropyridines by Using Gas-Phase Ion-Molecule Reactions; Anyin Li1; Jinshan Gao1; Mingkun Fu2; Bart omiej Jankiewicz3; John Nash1; Hilkka Kenttamaa1; 1Chemistry Department, West Lafayette, IN; 2Millennium, Cambridge, MA; 3Military University of Technology, Warsaw, Poland

ThP 610 Comparison of the Reactivity of Isomeric Charged Phenyl Radicals toward Tetrahydrofuran in Solution and in the Gas Phase; Fanny Widjaja1; Anthony Adeuya2; Hilkka Kenttamaa3; 1Purdue University, West Lafayette, IN; 2FDA, Little Rock, AR; 3Chemistry Department, West Lafayette, IN

ThP 611 Guided Ion Beam Mass Spectrometry Determination of Thorium Carbide and Thorium Oxide Cation Bond Energies; Richard Cox; Peter B. Armentrout; University of Utah, Salt Lake City, UT

ThP 612 Generation of Distonic Dehydrophenoxide Radical Anions From In-Source Dissociation; Kiran Kumar Morishetti1; Prabhakar Sripadi2; Vairamani Mariappanadar2; Jianhua Ren1; 1University of The Pacific, Stockton, CA; 2Indian Institute of Chemical Technology, Hyderabad, India

ThP 613 Gas Phase Alpha-Effect in SN2 and E2 Mechanisms;John Garver1; Veronica M. Bierbaum 2; 1University of Colorado, Boulder, Boulder, CO; 2University of Colorado, Boulder, CO

ThP 614 A Radical Approach to Ion Conformation Determination: Electron Transfer Dissociation, ion Mobility and Molecular Modelling; Iain D G Campuzano1; Frantisek Turecek2; Jeff Brown 1; Keith Richardson1; 1Waters Corporation, Manchester, UK; 2University of Washington, Seattle, WA

ThP 615 Mass Spectrometric Studies of Complex Organocatalyzed Reactions in Solution and Gas


Phase Using Triple Quadrupole MS and FT-ICR MS;Mhd Wasim Alachraf; Wolfgang Schrader; Max-Planck Inst Coal Res., Mülheim / Ruhr, Germany

ThP 616 How the Shape of an H-Bonded Network Controls Proton-Coupled Water Activation in HONO Formation; Michael Kamrath1; Rachael Relph1;Timothy Guasco1; Ben Elliot1; Anne McCoy2; Ryan Steele1; Daniel Schofield3; Kenneth Jordan3; Albert Viggiano4; Eldon Ferguson5; Mark Johnson1; 1Yale University, New Haven, CT; 2The Ohio State University, Columbus, Ohio; 3University of Pittsburgh, Pittsburgh, Pennsylvania; 4AFRL, Hanscom Afb, MA; 5National Oceanic and Atmospheric Administration, Boulder, CO

ThP 617 Revealing Evidences for a Non-concerted Mechanism in a Aza-Diels-Alder Reaction: an Electrospray Ionization Mass spectrometry Investigation; Adão Sabino; Ângelo de Fátima; Rodinei Augusti; Maria Diniz; Daniel da Silva; Federal University of Minas Gerais, Belo Horizonte, Brazil

ThP 618 Charged-Reagents for Mechanism Studies of Organic Reactions by ESI-MS/MS – Insights into the Hantzsch Reaction; Vanessa Gonçalves Dos Santos1;Boniek G Vaz1; Rodrigo O M A de Souza2; Simon J Garden2; Marcos N Eberlin1; 1ThoMSon Mass Spectrometry Laboratory - UNICAMP, Campinas, Brazil; 2Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

ThP 619 Gas-Phase and Computational Studies of SN2 and E2 Reactions of Alkyl Halides; Keyanna Conner; Andrew Alexander; Scott Gronert; Virginia Commonwealth, Richmond, VA

ThP 620 FT-ICR-MS studies of Metal Cluster Ions of Insoluble Metal Sulfides formed Externally by Electrospray and Ion Chemistry; Kaitlin Papson 1;Jeffrey Spraggins2; Douglas P. Ridge1; 1University of Delaware, Elkton, MD; 2Vanderbilt University, Nashville, TN

ThP 621 Solvent-Nickel Complexes Coordination Reaction in Collision-Induced Dissociation Process; Xiaoji Cao; Zhejiang University of Technology, Hangzhou, China

ThP 622 The Iron Cation Catalyzed Dissociation of the EDTA-Fe(III) Complex in Gas Phase: A Combined CID-MS Experimental and Theoretical Investigation; Ke Zhi Jiang2; Leren Wan 3; Yuki Hashi3; Yuanjiang Pan1;Guoqiao Lai2; 1Department of Chemistry, Zhejiang University, Hang Zhou, China; 2Hangzhou Normal University, Hang Zhou, China; 3Shimadzu Shanghai Office, Shang Hai, China

INSTRUMENTATION: NEW CONCEPTS II, 623 - 641 ThP 623 Ruggedized Miniature Cylindrical Ion Trap Mass

Spectrometer for Field Deployable MS and MS/MS Analysis; James Fox; Guido F. Verbeck; University of North Texas, Denton, TX

ThP 624 Soft-Landing of Metal-Carbide Clusters by Rectilinear Ion Trap Mass Spectrometry; William Hoffmann; Stephen Davila; Guido F. Verbeck; University of North Texas, Denton, TX

ThP 625 Electrospray Ionization Using a Miniature Mass Spectrometer to Model in situ Analysis of Bio-organic Materials in Planetary Ices; Ewa Sokol1; Robert J. Noll1; R. Graham Cooks 1; Luther W. Beegle2; Hugh Kim2; Isik Kanik2; 1Purdue University, West Lafayette, IN; 2Jet Propulsion Laboratory, Pasadena, CA

ThP 626 Fast, High Resolution Imaging Mass Spectrometry Using a Medipix Pixilated Detector; Julia Jungmann;Luke Macaleese; Ronald Buijs; Frans Giskes; Ad de

Snaijer; Ron M.A. Heeren; FOM-Institute Amolf, Amsterdam, Netherlands

ThP 627 Charge Detection Mass Spectrometry of Protein Complexes; John Smith; Craig Hollars; Joshua Maze; Martin Jarrold; Indiana University, Bloomington, Indiana

ThP 628 High Pressure Ion Detection for Miniaturized Mass Spectrometers; Travis M. Falconer1; Derek Wolfe1;Collin McKinney1; M. Bonner Denton2; J. Michael Ramsey1; 1University of North Carolina, Chapel Hill, NC; 2University of Arizona, Tucson, AZ

ThP 629 Focal Plane Array Detectors on a Multi-collector ICPMS – Preliminary Results; Charles J. Barinaga1;David W. Koppenaal1; George Hager1; Anthony Carado1; M. Bonner Denton2; Roger Sperline2; Gary M. Hieftje3; Steven Ray3; Jeremy Felton3; Gregory Schilling4; Gene Atlas5; 1Pacific Northwest Nat'l Laboratory, Richland, WA; 2University of Arizona, Tucson, AZ; 3Indiana University, Bloomington, IN; 4Leco Corporation, St. Joseph, MI; 5ImagerLabs,Monrovia, CA

ThP 630 Development of Tripole-Multipole Rotational Ion Mobility Mass Spectrometer by Ion Simulations;Takanori Harada; Tsutomu Masujima; Hiroshima Univ. BioMed., Hiroshima, Japan

ThP 631 Immobilization of Organometallic Complexes on Self Assembled Monolayers through Ligand Stripping and Reactive Landing of Mass Selected Ions; Grant Johnson1; Julia Laskin2; 1Pacific Northwest National Laboratory, Richland, WA; 2Pacific NW National Laboratory, Richland, WA

ThP 632 Biomolecular Ion Accelerator; Yun-Fei Hsu1, 2; Jung-Lee Lin2; Yi-Sheng Wang2; Chung-Hsuan Chen1, 2;1Department of Chemistry,National Taiwan University, Taipei, Taiwan; 2Genomics Research Center, Academia Sinica, Taipei, Taiwan

ThP 633 Surface Studies Using Soft- and Reactively Landed Ions on Plasma Pretreated Silver Targets; Karl E. Jackson; Christopher Moss; Frantisek Turecek; University of Washington, Seattle, WA

ThP 634 A New Conjoined RF Ion Guide for Enhanced Ion Transmission; Kevin Giles; David Gordon; Waters Corporation, Manchester, UK

ThP 635 Engineered Nanofilms for improved MCP performance in Mass Spectrometry applications;David Beaulieu; Philippe de Rouffignac; Dmitry Gorelikov; Kourosh Saadatmand; Ken Stenton; Neal Sullivan; Anton Tremsin; Arradiance Inc, Sudbury, MA

ThP 636 VUV Synchrotron Radiation: a new activation technique for Quadrupole Ion Trap Tandem Mass Spectrometry; Aleksandar Milosavljevic1, 3; Alexandre Giuliani 1, 2; Christophe Nicolas1; Jean-Francois Gil1;Matthieu Refregiers1; Laurent Nahon1; 1Synchrotron SOLEIL, Gif-Sur-Yvette Cedex, France; 2Cepia, INRA, Nantes, France; 3Institute of Physics, Belgrade, Serbia

ThP 637 Charge Retention and Neutralization Kinetics during Ion Soft Landing; Guangtao Li; Liang Gao ; R. Graham Cooks; Purdue University, West Lafayette, IN

ThP 638 Pulsed Multiple Reaction Monitoring Mode: A Novel Sensitive Approach to Biomolecule Quantitation;Mikhail Belov; Satendra Prasad; David Prior; William F. Danielson ; Karl Weitz; Vladislav A Petyuk; Yehia Ibrahim ; Richard D. Smith; Pacific Northwest National Laboratory, Richland, WA

ThP 639 Analysis of Mammalian Cell Suspensions Using Aerosol Time-of-Flight Mass Spectrometry; Matt


Spencer1; Thomas Darlington1; John Holecek1; Oanh Nguyen1; Andy Smith1; Steve Oldenburg1; Kimberly Prather2; 1Nanocomposix Inc., San Diego, CA; 2University of California San Diego, San Diego, CA

ThP 640 One-Dimensional Detector Array (IonCCDTM) as Position-Sensitive Anode for a Multi-Channel Plate (MCP); Omar Hadjar; Gottfried Kibelka; Chad Cameron; Scott Kassan; Scott Shill; O.I. Analytical, Pelham, AL

ThP 641 LC-MRM3 and LC-MS3: Alternative Strategies for Highly Selective Quantitation and for Sensitive Additional Structural Elucidation; Roland Geyer1;Axel Besa2; Christof E. Lenz2; 1Tecan Group Ltd., Männedorf, Germany; 2AB Sciex, Darmstadt, Germany

INSTRUMENTATION QUADRUPOLES AND TRAPS, 642 - 672

ThP 642 Hybrid ESI-Q-Ion Trap-TOF for Infrared Photodissociation Experiments; Kerim Gulyuz; Nathan P. Roehr; Da Wang; Nick C. Polfer; University of Florida, Gainesville, FL

ThP 643 Tutorial: Prototyping Architectures for Ion Trap Mass Spectrometers; Matthew A Kirleis; Wei Xu; R. Graham Cooks ; Zheng Ouyang; Purdue University, West Lafayette, IN

ThP 644 A Tightly Curved Collision Cell with High MSMS Efficiency; Felician Muntean; Varian Inc., Walnut Creek, CA

ThP 645 Tandem Linear Ion Traps – A Novel Technique to Analyze Large Populations of Ions; Mircea-Michael Guna; Frank A. Londry; AB SCIEX, Concord, Canada

ThP 646 A New Linear Quadrupole Ion Trap with Axial Ejection; David Langridge; Jason L Wildgoose; Waters Corporation, Manchester, UK

ThP 647 Exploring the Limits of High Resolution Excitation in a Low Pressure Linear Ion Trap; Bruce Collings; AB SCIEX, Concord, Canada

ThP 648 Linear Ion Trap Mass Spectrometry for Large Biomolecular Ions by MALDI; I-Chung Lu; Jung-Lee Lin; Chung-Hsuan Chen; Genomics Research Center, Academia Sinica, Taipei, Taiwan

ThP 649 Mass Spectrometric Capabilities of a Novel Instrument for the Spectroscopic Characterization of Biomolecular Ions; James Redwine; Scott A. Mcluckey; Purdue University, West Lafayette, IN

ThP 650 Rapid Detection of Positive and Negative Electrospray Ions Using Millisecond Polarity Switching and a Static Unifield Detector; Lisa M. Cousins; Heather Gamble; Charles Jolliffe; Victor Titov; Dmitry Valyaev; IONICS Mass Spec Group, Inc., Bolton, ON

ThP 651 ScanWave Enabled MS3 Analysis on a Tandem Quadrupole Mass Spectrometer; Daniel J Kenny; Waters Corporation, Manchester, UK

ThP 652 Implementation of ETD Reactions in a Linear Quadrupole Collision Cell Utilizing a Static DC Gradient for Dual Ion Trapping; Dirk Nolting; Jens Griep-Raming; Robert Malek; Alexander Makarov; Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany

ThP 653 Rectilinear Quadrupole Ion Guides: Effect of Rod Size and RF Frequency on Transmission as a Function of Mass; Christopher Taormina; Randy Pedder; Ardara Technologies L.P., Ardara, PA

ThP 654 A Linear-type Ion Trap Realized with Two Lithographically Patterned Substrates; Brett Hansen;

Hannah Quist; Brandon Barney; Aaron Hawkins; Daniel Austin; Brigham Young University, Provo, Utah

ThP 655 A Digital Linear Ion Trap based on Ceramic Printed Circuit Boards; Mu Hui; GongYu Jiang; Tao Lin; XiaoHui Yang; JunSheng Zhang; Li Ding; Shimadzu Research Laboratory (Shanghai), Shanghai, China

ThP 656 Modeling the Ion Funnel Trap for Ion Mobility Spectrometry; Aleksey V. Tolmachev1; Yehia Ibrahim 2; Richard D. Smith3; Mikhail Belov4; 1Pacific Northwest National Lab, Richland, WA; 2Pacific Northwest National Laboratory, Richland, WA; 3PNNL,Richland, WA; 4Pacific Northwest Nat Lab, Richland, WA

ThP 657 Short-Pulse Ion Extraction from a Quadruple Ion Trap into a Time-of-Flight Mass Spectrometer for Trapped Ion Cloud Diagnostics; Beni B. Dangi;Nicholas A. Sassin; Kent M. Ervin; University of Nevada, Reno, Reno, NV

ThP 658 Simultaneous Injection and Ejection with a Linear Ion Trap for High Detection Efficiency; Yuichiro Hashimoto; Hiroyuki Satake ; Masuyuki Sugiyama; Hideki Hasegawa; Hitachi, Ltd, Central Research Lab, Kokubunji, Tokyo, Japan

ThP 659 Profiling Two-Dimensional Ion Cloud Distribution in a Quadrupole Ion Trap Mass Spectrometer with Infrared Multiphoton Photodissociation; G. AsherNewsome; Alfred Z. Zhong; Gary L. Glish; University of North Carolina, Chapel Hill, NC

ThP 660 Investigations to Improve the Trapping and Ejection Characteristics of a Toroidal RF Ion Trap Using Simulations and Modified Electrode Geometries;Steve Lammert; Joe Oliphant; Edgar Lee; TorionTechnologies, Inc, American Fork, UT

ThP 661 Kinetic Energies of Ions Ejected Radially from LIT at High q; Viatcheslav V. Kovtoun; Thermo Fisher Scientific, San Jose, CA

ThP 662 Optimization of Multipole Components in a Planar Paul Trap; Zhiping Zhang; Ying Peng; Hannah Quist; Junting Wang; Brett J. Hansen; Aaron R. Hawkins; Daniel E. Austin; Brigham Young University, Provo, UT

ThP 663 Miniaturization of an Axially Focusing Linear Ion Trap for Mass Spectrometry; Gareth Dobson1;Christie Enke2; 1SRI International, Menlo Park, CA; 2University of New Mexico, Albuquerque, New Mexico

ThP 664 A Full Featured, Flexible, and Inexpensive 2D and 3D Ion Trap Control Architecture and Software Package; Ryan M. Danell1; Friso H.W. Van Amerom2;Veronica Pinnick3; Timothy J. Cornish4; Joel H. Parks5;Luann Becker6; 1Danell Consulting, Greenville, NC; 2SRI International, St Petersburg, FL; 3Johns Hopkins School of Medicine, Baltimore, MD; 4C&E Research, Inc., Columbia, MD; 5Harvard University, Cambridge, MA; 6Johns Hopkins University, Baltimore,MD

ThP 665 Development of an Atmospheric Mass Spectrometer for Universal Real-Time Monitoring Applications;Karl Weitz1; Ronald Moore1; Kim K. Hixson1; Daniel Lopez-Ferrer1; C. Mark Taylor2; Smith Richard1;1Pacific Northwest National Laboratory, Richland, WA; 2Shimadzu Scientific, Inc.,, Columbia, Maryland

ThP 666 Development and Improvement of Miniature Quadrupole Array Mass Spectrometers; Kohei Sasai 1, 3; Jun Aoki1; Hideyuki Iwata2; Michisato Toyoda1;1Osaka University, Toyonaka, Japan; 2Implant Sciences Corp., San Diego, CA; 3HORIBA Stec, Kyoto, Japan

ThP 667 Reduced Scale Rectilinear Ion Trap Arrays; Paul Hendricks1; Jeff Maas2; William Chappell2; Zheng


Ouyang1; R. Graham Cooks 1; 1Dept. Chemistry Purdue University, West Lafayette, IN; 2Dept. EE Purdue University, West Lafayette, IN

ThP 668 In situ Optimization of Microscale Cylindrical Ion Trap Geometry; Derek W. Wolfe1; Travis M. Falconer1; Joe Matteo2; J. Michael Ramsey1; 1University of North Carolina, Chapel Hill, NC; 2MicroTypes, Walland, TN

ThP 669 Development of a Backpack Mass Spectrometer with an Extended Probe; Keyong Hou1; Wei Xu2; Jian Xu2;Tsung-Chi Chen2; R. Graham Cooks1; Zheng Ouyang2;1Department of Chemistry,Purdue University, West Lafayette,, IN; 2Weldon School of BME, Purdue University, West Lafayette, IN

ThP 670 Planar Technologies for Optimized Realizations of Quadrupole Ion Guides and Quadrupole ion Wave Guides; Albrecht Glasmachers1; Alexander Laue1;Albrecht Brockhaus1; Ralph Pulwey2; Michel Aliman2;1University of Wuppertal, Wuppertal, Germany; 2CarlZeiss NTS GmbH, Oberkochen, Germany

ThP 671 Multiplexed MS/MS in a Miniature Rectilinear Ion Trap; Adam M Graichen; Richard Vachet; University of Massachusetts, Amherst, MA

ThP 672 Compact and High Efficient Secondary Ion Mass Spectrometer with Quadrupole Traps for Electron Microscopes to Combine Structural and Analytical Imaging; Alexander Laue1; Albrecht Glasmachers1;Albrecht Brockhaus1; Michel Aliman2; Hubert Mantz2;1University of Wuppertal, Wuppertal, Germany; 2CarlZeiss NTS GmbH, Oberkochen, Germany

thursday posters bioinformatics iv, 001 - 021 · 2017. 1. 29. · page s122 58th asms conference on...

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