thrombophilia testing and management - htrs · tpa=tissue plasminogen activator; pai-1=plasminogen...
TRANSCRIPT
![Page 1: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/1.jpg)
Stephan Moll, MD
Medicine, Heme-Coag
UNC Chapel Hill, NC
HTRS workshop
April 13th 2016
Thrombophilia
Testing and Management
12
3
4 5APLA
ACALA
Anti-ß2-GP I
![Page 2: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/2.jpg)
Disclosures
Financial Conflicts of Interest: NONE
Off-label product use discussion: NONE
![Page 3: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/3.jpg)
Pre-test Question 1
A 32-year-old man is seen for evaluation for a
possible hypercoagulable state. His father was
recently diagnosed with heterozygous factor V
Leiden mutation following an episodes of unprovoked
venous thromboembolic disease. The patient is
concerned with his own risk of thrombosis. Medical
history is negative, with no venous
thromboembolism.
![Page 4: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/4.jpg)
![Page 5: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/5.jpg)
Pre-test Question 2
A 28-year-old woman presents with a 2-day history of right
leg pain and swelling. Medical history is unremarkable. Her
only medication is an estrogen-progestin oral contraceptive
pill. She is diagnosed with a proximal deep venous
thrombosis; there was no preceeding trauma, surgery,
immobility or long-distance travel. Anticoagulant therapy with
low-molecular-weight heparin and warfarin is initiated. A
thrombophilia evaluation is ordered, which includes factor V
Leiden and prothrombin G20210A genetic testing; protein C,
S, and antithrombin activities; lupus anticoagulant; and
anticardiolipin and anti-β2-glycoprotien-I antibody testing.
![Page 6: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/6.jpg)
![Page 7: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/7.jpg)
Pre-test Question 3
A 63 year old otherwise healthy man undergoes left
hip replacement surgery for osteoarthritis. He is
making a good recovery, but 2 weeks after the
surgery he develops left leg swelling and pain and is
diagnosed with a proximal left leg DVT. How long
should he be treated with anticoagulation?
![Page 8: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/8.jpg)
![Page 9: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/9.jpg)
Introduction / The Basics
2 3 41
![Page 10: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/10.jpg)
![Page 11: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/11.jpg)
VTE: How Long To Anticoagulate?
Define the clot
R leg proximal DVT, unprovoked
1
List the VTE risk factors: (a) …, (b) …, (c) ...
(a) OCP, (b) BMI, (c) hetero FVL
2
![Page 12: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/12.jpg)
How Long to Treat With Anticoagulation?
0 10
Warfarin “Hate Factor”
Blood Thinner “Dislike Factor”
3. Patient preference
2. Risk for Bleeding
(a)…., (b)…., (c) …..
1. Risk of recurrent VTE
(a)…., (b)…., (c) …..
Conglomerate decision of:
[MacLean S et al. Chest. 2012;141(2_suppl):e1S-e23S]
![Page 13: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/13.jpg)
Case
• 27 yr old woman
• PE and prox + distal DVTVTE risk factors:
a) OCP (estrogen/progestin)
b) BMI > 30.
Question: How long to treat with anticoagulation?
![Page 14: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/14.jpg)
VTE due to major transient risk factor
Woman with unprovoked VTE
Woman with VTE on hormones
Long-term
3 months
Recurrence Triangle
• PE
• DVT
Man with unprovoked VTE
Non-major transient risk factor
• PE
• DVT
Stro
ng
Th
rom
bo
ph
ilia
D-d
ime
r
+
-
[Choosing Wisely®;
Hicks LK, et al. Hematology Am Soc
Hematol Educ Program. 2014;2014:
599-603]
ACCP, AHA, ISTH, BJH, ACF
![Page 15: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/15.jpg)
VTE due to major transient risk factor
Woman with unprovoked VTE
Woman with VTE on hormones
Long-term
3 months
• PE
• DVT
Man with unprovoked VTE
Non-major transient risk factor
• PE
• DVT
Recurrence Triangle
1 year
1 % 3 %
5 % 15 %
10 % 30 %
5 years
Cumulative VTE
Recurrence Rate
[Kearon C et al. Blood 2014;123:1794-1801]
6 % *
*[Douketis J et al. BMJ 2011;342:d813]
Stro
ng
Th
rom
bo
ph
ilia
D-d
ime
r
+
-
![Page 16: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/16.jpg)
• 3.6 % of recurrent VTE are fatal
• 10 % of major bleeds are fatal
Case Fatality
Thus:
If risk of recurrence ≥ 3x higher than risk of major bleed =
long-term anticoagulation.
[Kearon C, Akl EA. Duration of anticoagulant therapy for DVT and PE Blood. 2014 Mar 20;123(12):1794-801]
![Page 17: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/17.jpg)
Recurrent VTE and Major Bleed
[Kearon C, Akl EA. Duration of anticoagulant therapy for DVT and PE Blood. 2014 Mar 20;123(12):1794-801]
![Page 18: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/18.jpg)
VTE due to major transient risk factor
Woman with unprovoked VTE
Woman with VTE on hormones
Long-term
3 months
• PE
• DVT
Man with unprovoked VTE
Non-major transient risk factor
• PE
• DVT
Fatality
Recurrent
VTE
3 % 1 of 1,250
15 % 1 of 170
30 % 1 of 80
Case
fatality
at 5 years
1 of 600S
tron
g
Th
rom
bo
ph
ilia
D-d
ime
r
+
-6 %
[Kearon C, Akl EA. Duration of anticoagulant therapy for DVT and PE Blood. 2014 Mar 20;123(12):1794-801]
![Page 19: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/19.jpg)
1. APLA syndrome
2. Antithrombin deficiency
3. Protein C deficiency
4. Protein S deficiency
5. Homozygous factor V Leiden
6. Homozygous II20210 mutation
7. Heterozygous FVL plus heterozygous II20210
“Strong” Thrombophilias
Yes
yes / no
unknown
yes / no
[Segal J et al. JAMA 2009; 301:2472-85]
[Lijfering WM et al. Circulation 2010;121:1706-12]
[Garcia D et al. Blood 2013;122:817-824]
![Page 20: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/20.jpg)
Individual Thrombophilias…
1 32 4
…Key Points
![Page 21: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/21.jpg)
[Pomp ER, et al. Br J Haematol. 2007;139:289-296]
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0.0
Od
ds r
ati
o
BMI
Obesity and VTE Risk
![Page 22: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/22.jpg)
• Factor V Leiden
• Prothrombin 20210
• Protein C deficiency
• Protein S deficiency
• Antithrombin deficiency
• Antiphospholipid antibodies
(ACA, LA, anti-β2-GPI)
• ↑ Homocysteine, MTHFR
• ↑ Fibrinogen, factor VIII, IX, XI
• PAI-1, tPA levels and polymorphisms
• CBC, CD55/59, JAK-2
Acquired
Most
common
Classics
Others
Inherited and Acquired Thrombophilias
![Page 23: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/23.jpg)
• Factor V Leiden, heterozygous: 1 : 20
• II20210 mutation, heterozygous: 1 : 50
• Protein C deficiency 1 : 500
• Protein S deficiency 1 : 500
• Antithrombin deficiency 1 : 5,000
• Homozygous protein C deficiency 1 : 250,000
Prevalences
[1. Koster T, et al. Lancet. 1993;342:1503-1506; 2. Poort SR, et al. Blood. 1996;88:3698-3703; 3. Tait RC, et al. Thromb Haemost. 1995;73:87-93; 4. Faioni EM,
et al. Thromb Haemost. 1997;78:1343-1346; 5. Patnaik MM, Moll S. Haemophilia. 2008;14:1229-1239. 6. www.nationalatlas.gov/articles/people/a_gender.html;
7. Report on the Annual Global Survey 2007. World Federation of Hemophilia, 2008]
• Homozygous FVL 1 : 1,600
• Hetero FVL + hetero II20210 1 : 1,000
• Homozygous II20210 1 : 10,000
![Page 24: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/24.jpg)
Risk of 1st VTE with thrombophilias
[Mo
ll S
. J T
hro
mb
Th
rom
boly
s2
01
5;;3
9(3
):3
67-7
8]
Relative risk increase
for first VTE
No thrombophilia Reference group
II20210, hetero 3.8 (95% CI 3.0-4.9)
FVL, heterozygous 4.9 (95% CI 4.1-5.9)
II20210, homozygous Insufficient data
FVL, homozygous 18 (95% CI 4.1-41)
Hetero II20210 PLUS
hetero FVL
20 (95% CI 11.1-36.1)
Protein S deficiency 30.6 (95% CI 26.9-55.3)
Protein C deficiency 24.1 (95% CI 13.7-42.4)
Antithrombin deficiency 28.2 (95% CI 13.5-58.6)
![Page 25: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/25.jpg)
Risk of Recurrent VTE with Thrombophilias
(95% CI 0.96-2.21)II20210, hetero: 1.45
(95% CI 1.14-2.12)FVL, hetero: 1.561
[Segal J et al. JAMA 2009; 301:2472-85]
(95 % CI 1.18-5.97)FVL, homo: 2.65
[Segal J et al. JAMA 2009; 301:2472-85; meta-analysis]
FVL, homo: 1.2 (95 % CI 0.5-2.6)
[Lijfering WM et al. Circulation 2010;121:1706-12]
2
(95 % CI 0.50-46.3)FVL + II2010: 4.81
[Segal J et al. JAMA 2009; 301:2472-85; meta-analysis]
FVL + II2010: 1.0 (95 % CI 0.6-1.9)
[Lijfering WM et al. Circulation 2010;121:1706-12]
3
II20210, homo: insufficient data4
![Page 26: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/26.jpg)
Protein C
Protein S
Antithrombin
2.8 (95 % CI 2.0 – 4.0)
[Lijfering WM et al. Circulation 2010;121:1706-12]
5
[Garcia D et al. Blood 2013;122:817-824]
(95 % CI 0.99-2.00)
(95 % CI 0.76-3.11)
(95 % CI 0.83-9.64)
APLA: 1.41
ACA: 1.53
LA: 2.83
APLA syndrome:[Kearon C et al; Chest 2012;141:e419S-494S]
2.0
6
Risk of Recurrent VTE with Thrombophilias
![Page 27: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/27.jpg)
Acquired deficiency:
• Liver disease (C, S, AT)
• Warfarin therapy (C, S)
• Estrogens, pregnancy (S)
• Inflammatory diseases (S)
• Heparin therapy (AT)
• Acute thrombosis (S, AT)
Practical point:
• Always question the diagnosis!
Protein C, S and Antithrombin Deficiency
How do you test?
• C, S, AT activity
• free S antigen
• >100 mutations
How do you treat?
• Consider AT concentrate
• Severe neonatal C deficiency: protein C
concentrate
![Page 28: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/28.jpg)
APLA
ACALA
Anti-ß2-GP I
I) Antibody test (ELISA)
• anticardiolipin
• anti-ß2-glycoprotein I
• antiphosphatidylserine
• antiphosphatidylcholine
• antiphosphatidylethanolamine
II) Functional test
• lupus anticoagulant (inhibitor)
[Sapporo criteria: Miyakis S. J Thromb Haemost. 2006;4:295-306]
Antiphospholipid Antibody Testing
![Page 29: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/29.jpg)
PL -β2-GP-I
• Up to 10% of pts with APLA syndrome only
positive for anti-β2-GP I
Antiphospholipid Antibodies
[Myers B et al. Blood Coag Fibrinolys 2003;14:1-2]
[Cabral AR et al. Am J Med 1996;101:472-81]
[Miyakis S et al. J Thromb Haemost 2006;4:295-306]
• Β2-GP-I Ab may be more specific for APLA syndrome
• Not yet recommended that anti-β2-GP-I Ab test replaces ACA
[Lakos G et al. Arthritis Rheumatism 2012;64:1-10]
• IgG and IgM recommended (for ACA and anti-β2-GP-I Ab);
• if neg: test for IgA.
[Lakos G et al. Arthritis Rheumatism 2012;64:1-10]
• “Testing for IgA Abs is NOT recommended”[Keeling D et al. Br J Haematol 2012;157:47-58]
![Page 30: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/30.jpg)
An
tib
od
y t
ite
r
Time
Antiphospholipid Antibodies
![Page 31: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/31.jpg)
[Miyakis S, et al. J Thromb Haemost. 2006;4:295-306].
Updated Sapporo Criteria for APS (2006):
Clinical criteria:
– Vascular thrombosis
– Pregnancy morbidity
Laboratory criteria*:
– Anticardiolipin antibodies (IgG or IgM)
(medium or high titer: >40 GPL/MPL or >99th percentile)
– Anti-β2-glycoprotein I antibodies (IgG or IgM) (>99th percentile)
– LA
*2 or more occasions, >12 wks apart.
APLA: Sapporo/Sidney Criteria
![Page 32: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/32.jpg)
• “APLA syndrome”: question the diagnosis
• moderately high titers of ACA (40 U/mL)
• Include anti-β2-GP-I antibody testing
• Test outside the acute event
• Repeat APLA testing (3 months apart)
• Know the LA tests and their interpretation
APLA: Practical Points
• Role of IgA antibodies unclear.
![Page 33: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/33.jpg)
ACA +; anti-β2-GP-I +; LA +
[Galli M, et al. Blood. 2003;101:1827-1832;
Ruffatti A, et al. Thromb Haemost. 2006;96:337-341].
Conclusions:
1. LA stronger and more
definitive risk factor for
thrombosis than ACA.
2. Risk for thrombosis is
highest if positive APLA by
multiple assays.
APLA: Risk for 1st Thrombosis
![Page 34: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/34.jpg)
Are APLA a Risk Factor for Recurrent VTE?
[Garcia D et al. Blood 2013;122:817-824]
• 8 eligible studies
• All had important methodologic limitations.
• “We judged the overall quality of the evidence as very low”.
Systematic Review
• Although it appears that pos. APLA predict ↑’d risk of VTE
recurrence, the strength of this association is uncertain.
Conclusion
![Page 35: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/35.jpg)
Risk for Recurrent VTE
[Garcia D et al. Blood 2013;122:817-824]
1. Are APLA a risk factor for recurrent VTE? “They appear to be!”
![Page 36: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/36.jpg)
Risk for Recurrent Thrombosis
2. Which of the APLA best predicts recurrent VTE?
[Garcia D et al. Blood 2013;122:817-824]
LA
ACA
![Page 37: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/37.jpg)
Practical point:
Don’t Test for MTHFR.
Practical point:
Test for homocysteine?– no good indication.
• MTHFR C677T, homozygous TT, or A1298C
• May have higher homocysteine levels
• Not a risk factor for thrombosis or pregnancy complications
Homocysteine, MTHFR
MTHFR=methylenetetrahydrofolate reductase
[den Heijer MJ. Thromb Haemost. 2005;3:292-299]
[Klerk M. JAMA. 2002;288:2023-2031]
[Rey E. Lancet. 2003;361:901-908
[ACOG Bulletin #124, 2011]
![Page 38: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/38.jpg)
MTHFR
![Page 39: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/39.jpg)
tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.
].
• Elevation of factor VIII
• Elevations of fibrinogen, factors II, IX, XI
• Fibrinolysis abnormalities:
– Plasminogen deficiency
– Decreased tPA levels and polymorphisms
– Elevated PAI-1 level and polymorphisms
– Elevated TAFI levels
• Myeloproliferative disorders (JAK-2 mutation)
• Paroxysmal nocturnal hemoglobinuria (CD55/59)
Other Thrombophilias
![Page 40: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/40.jpg)
Unexplained Arterial Thrombosis
http://professionalsblog.clotconnect.org/2011/01/31/unexplained-arterial-
thrombosis-causes-thrombophilia-testing
1. Arteriosclerosis documented or risk factors present?
2. Cardioembolic source?
3. Other causes (hormones, cocaine, vasculitis, etc?)
4. Thrombophilia?
[Moll S. J Thromb Thrombolys 2015; ;39(3):367-78]
![Page 41: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/41.jpg)
Arterial Thrombosis
FVL: OR 1.21 (95% CI, 0.99-1.49)
II20210: OR 1.32 (95% CI, OR 1.03-1.69)
Protein C and S deficiency
• <55 yrs: 4.7-fold (95 % CI 1.5-4.2)
• >55 yrs: 1.1-fold (95 % CI 1.1-18.3)
Antithrombin deficiency
• “Not a risk factor”
Mahmoodi BK, et al. Circulation. 2008;118:1659-1667]
[Kim RJ et al. Am Heart J 2003;146:948-957]
![Page 42: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/42.jpg)
Unexplained Arterial Thrombosis
How to best treat (secondary prevention?
1. Anti-platelet therapy?
2. Anticoagulant?
3. Both together?
The more information, the better – even though we don’t have all the answers.
![Page 43: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/43.jpg)
1 2 3 4
Whom to Test for Thrombophilia?
![Page 44: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/44.jpg)
Whom to Test for Thrombophilia?
Background:
3 reasons to consider testing:
1. “Does it influence length of anticoagulation decision?
2. “Does it have consequences for patient’s family members?”
3. Wish of patient and MD to “understand” why clot happened
![Page 45: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/45.jpg)
Any pt with
spontan. VTE
Young pt
with FHx
Any pt
with VTE
Everybody Young pt Nobody
Whom to Test for Thrombophilia?
Arch-conservativeUltra-liberal
![Page 46: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/46.jpg)
Am Coll Med
Geneticists, 2001
Europ Genetics Foundation,
Mediteranean League on
Thromboembolism, Internat. Union
of Angiology, etc., 2005
Thrombosis Interest
Group of Canada 2006
CAP, 2002
[van Cott EM et al. Arch Pathol Lab Med 2002;126:1281-1295]
[Nicolaides AN. International Angiology 2005;24:1-26]
[Mant M. www.tigc.org/eguidelines/hypercoagstates.htm]
[Grody WW. Genetics in Medicine 2001;3:139-148]
Whom to Test for Thrombophilia?
[Baglin T. Br J Hematol 2010;Jan 28;Epub ahead of print]
Arch-conservativeUltra-liberal
[EGAPP. Genetics in Medicine 2011;13 (1)67-76]
EGAPP 2011
Br Committee for Standards in
Haematol, 2010
ACF, 2016
[Stevens S et al. J Thromb Thromblys 2016]
![Page 47: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/47.jpg)
Whom to Test
• Controversial
• Recent guideline: ACF 2016
• Guidelines in development: ASH 2016/2017
• Terminology used:
• “testing can be considered”
• “benefit of testing unclear”
• “lack of evidence that testing impacts clinical outcomes”
• Cost, mis-interpretation
Stevens S et al. J Thromb Thrombolys 2016]
![Page 48: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/48.jpg)
Who Should Test? When to Test?
[Cushman M. Clin Chem 2014;50:134-7]
• Patient selection
• Pretest counseling
• Proper lab test interpretation
• Provision of education and advice
1. Only the professional knowledgeable about the 4Ps should test.
2. Do not test while patient is on an anticoagulant.
3. Do not test during acute thrombotic episode.
4. Do not test a hospitalized patient.
![Page 49: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/49.jpg)
1
2
3
4
5
6
DVT/PE, intermediate risk recurrence
VTE in unusual locations, unprovoked
Arterial thrombosis, unexplained
Pregnancy loss(es), unexplained
VTE: Patient requests testing
Family members (if “strong thrombophilia” in index pt)
In Whom to Consider Testing
![Page 50: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/50.jpg)
Choosing Wisely® Campaign
Recommend against thrombophilia testing in pts with VTE
associated with major transient risk factor.
Whom NOT to test
[Hicks LK et al. Blood. Hematology Am Soc Hematol Educ Program. 2013:9-14]
![Page 51: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/51.jpg)
What to Test?
1 2 3 4
![Page 52: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/52.jpg)
• Factor V Leiden
• Prothrombin 20210
• Protein C deficiency
• Protein S deficiency
• Antithrombin deficiency
• Antiphospholipid antibodies
(ACA, LA, anti-β2-GPI)
• ↑ Homocysteine, MTHFR
• ↑ Fibrinogen, factor VIII, IX, XI
• PAI-1, tPA levels and polymorphisms
• CBC, CD55/59, JAK-2
Acquired
Most
common
Classics
Others
[Foy P, Moll S. Curr Treat Options Cardiovasc Med. 2009;11:114-128]
Inherited and Acquired Thrombophilias
![Page 53: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/53.jpg)
Influence of Anticoagulants on Thrombophilia Tests
[Moll S. J Thromb Thrombolys 2015;39:367-378]
![Page 54: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/54.jpg)
Which Family Members to Test for Thrombophilia
1 2 43
![Page 55: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/55.jpg)
Which Family Members to Screen for Thrombophilia?
• Having a 1st degree relative with VTE, increases a family member’s risk
for VTE.
• Increased risk is irrespective of presence of FVL or prothrombin 20210:
• Positive family history but no FVL or prothrombin 20210: OR 2.6
(95% CI, 1.7-3.8)
• Positive family history plus FVL or prothrombin 20210: OR 3.6
(95% CI, 1.2-4.0)
[Noboa S, et al. Thromb Res. 2008;122:624-629]1. [Bezemer ID, et al. Arch Intern Med. 2009;169:610-615]
![Page 56: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/56.jpg)
Which Family Members to Consider for Thrombophilia Screening?
Proband’s
thrombophiliaMale Family Member
Sons Brothers
Hetero FVL or hetero
prothrombin 20210no no
Homo FVL or homo
prothrombin 20210no reasonable
Double heteroreasonable reasonable
C, S, ATreasonable reasonable
Female Family Member
Daughters Sisters
no no
no yes
yes yes
yes yes
“Reasonable” because: consider LMWH with airline travel, cast, non-major surgery;
prolonged after major surgeries.
“reasonable
”
“Yes” because: advise against estrogen contraceptives/hormone therapy; give ante-
and postpartum anticoagulation.
“yes”
![Page 57: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/57.jpg)
Pregnancy Loss / Complications
Anticoagulation in Pregnancy Complications
“Whether anticoagulant therapy prevents recurrent miscarriage in
women with inherited thrombophilia is controversial – inconsistent
results from trials.”
[Middeldorp S. Hematology 2014; ASH Education Program:393-399]
[www.trialregister.nl , NTR3361]
![Page 58: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/58.jpg)
• “Some asymptomatic women of fertile age”:
• may choose ante- and postpartum VTE prophylaxis
• may choose not to take hormonal contraceptives
[Middledorp S. J Thromb Thrombolys 2011;31:275-281]
• Benefit of testing and of LMWH treatment if thrombophilia
found is unclear.
• Enroll patients into trials; or: non-evidence-based decision.
Pregnancy Loss / Complications
![Page 59: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/59.jpg)
Progestin pill (minipill)
Non-hormonal methods
Estrogen combination pill
• 3rd generation
• 2nd generation
1
• Injectable progestins
• Depot preparation
• Rod
Drospirenone & ethinyl estradiol
1. Weiss G. Am J Obstet Gynecol. 1999;180:S295-S301.
2. Rosendaal FR, et al. Thromb Haemost. 2001;6:112-23.
3. Conard J, et al. Contraception. 2004;70:437-441.
4. Bergendal A, et al. Acta Obstet Gynecol Scand. 2009;88:261-266.
5. Barsoum M et al. Thromb Res 2010;126:373-8.
6. Van Hylkama-Vlieg A et al. Arterioscler Thromb Vasc Biol 2010;30:2297-300.
7. Mantha S et al. BMJ 2012;Aug 7;345:34944.
Progestin-releasing IUDs
Norelgestromin & ethinyl estradiol
Etonogestrel & estradiol ring
Contraceptive Options in Thrombosis / Thrombophilia
![Page 60: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/60.jpg)
Summary
1
2
3
Often little/no evidence of benefit of testing
When to test: At 3 months; not acutely, not inpatient, not
while on anticoagulation.
Whom to test? Terminology: “Consider”, not “recommend”
4 Cave: Interpretation of test results while on anticoagulation
![Page 61: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/61.jpg)
Summary: In Whom Do I consider Testing?
1
2
3
4
5
6
DVT/PE, intermediate risk recurrence
VTE in unusual locations, unprovoked
Arterial thrombosis, unexplained
Pregnancy loss, unexplained
VTE: Patient requests testing
Family members (if “strong thrombophilia” in index pt)
![Page 62: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/62.jpg)
Post-test Question 1
A 32-year-old man is seen for evaluation for a
possible hypercoagulable state. His father was
recently diagnosed with heterozygous factor V
Leiden mutation following an episodes of unprovoked
venous thromboembolic disease. The patient is
concerned with his own risk of thrombosis. Medical
history is negative, with no venous
thromboembolism.
![Page 63: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/63.jpg)
![Page 64: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/64.jpg)
Post-test Question 2
A 28-year-old woman presents with a 2-day history of right
leg pain and swelling. Medical history is unremarkable. Her
only medication is an estrogen-progestin oral contraceptive
pill. She is diagnosed with a proximal deep venous
thrombosis; there was no preceeding trauma, surgery,
immobility or long-distance travel. Anticoagulant therapy with
low-molecular-weight heparin and warfarin is initiated. A
thrombophilia evaluation is ordered, which includes factor V
Leiden and prothrombin G20210A genetic testing; protein C,
S, and antithrombin activities; lupus anticoagulant; and
anticardiolipin and anti-β2-glycoprotien-I antibody testing.
![Page 65: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/65.jpg)
![Page 66: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/66.jpg)
Post-test Question 3
A 63 year old otherwise healthy man undergoes left
hip replacement surgery for osteoarthritis. He is
making a good recovery, but 2 weeks after the
surgery he develops left leg swelling and pain and is
diagnosed with a proximal left leg DVT. How long
should he be treated with anticoagulation?
![Page 67: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/67.jpg)
![Page 68: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/68.jpg)
![Page 69: Thrombophilia Testing and Management - HTRS · tPA=tissue plasminogen activator; PAI-1=plasminogen activator inhibitor 1; TAFI=thrombin activatable fibrinolysis inhibitor.]. • Elevation](https://reader031.vdocuments.us/reader031/viewer/2022020105/5ca6ddc188c9935b378b6708/html5/thumbnails/69.jpg)
Questions?
Comments?