threshold of compound action potential - mahidol · character of compound action potential and...
TRANSCRIPT
1
thresholdpia.sfwdat : Page 1
durat:0.1ms A mpl:200mV
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 2
A mpl:220mV
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 3
A mpl:240
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 4
A mpl:260
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
thresholdpia.sfwdat : Page 5
A mpl:280
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 6
A mpl:300
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 7
A mpl:320
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 8
A mpl:340
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
2
thresholdpia.sfwdat : Page 5
A mpl:280
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 6
A mpl:300
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 7
A mpl:320
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 8
A mpl:340
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
Experiment: compound action potential
1 2 3 4 5 6 7 8
-+
PowerLab outputfor stimulation
To computer Dual BioAmp
1
3
Akk Threshold.sfwdat : Page 18
190 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 19
200 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 20
210 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 21
220 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potentialเริ่มเห็น action potential
Thresholdstimulus
Stimulusmarker
Stimulusartifact
Noisy background
2
Akk Threshold.sfwdat : Page 22
230 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 23
240 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 24
250 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 25
260 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential3
4
Akk Threshold.sfwdat : Page 26
270 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 27
280 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 28
290 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 29
300 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential4
Akk Threshold.sfwdat : Page 30
310 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 31
320 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 32
330 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 33
340 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential5
5
Akk Threshold.sfwdat : Page 34
350 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 35
360 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 36
370 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 37
380 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential6
Akk Threshold.sfwdat : Page 38
390 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 39
400 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 40
410 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 41
420 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential7
Maximal response
Maximalstimulus
6
Akk Threshold.sfwdat : Page 41
430 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Character of compound action potentialand conduction velocity (1)
stimulus
stimulusartifact
Late
ncy
(A)
Act
ion
pote
ntia
l dur
atio
n
Late
ncy
(a)
8
ข้ัวบันทึกคูใกล (4-5)
ข้ัวบันทึกคูไกล (6-7)
Conduction velocity =Latency ไกล - Latency ใกล
ขั้วบันทึกแรกของคูไกล - ขั้วบันทึกแรกของคู ใกล
Conduction velocity =a - A
6 cm – 4 cm
Zoom of Akk Threshold.sfwdat : Page 41
430 mV
-4
-2
0
2
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
amplitude (m
V) (mV)
0 2 4 6 8 10 12ms (ms)
Peak action potential ที่ขั้วบันทึก 4
Peak action potential ที่ขั้วบันทึก 6
เวลาที่ action potential ใชในการเดนิทางจากขั้ว 4 ถึง 6 (t)
Conduction velocity =t
6 cm – 4 cm
Conduction velocity (2)9
7
Biphasic action potential10
+- 0
+ -
+- 0
+ -
+ -
+- 0
Biphasic action potential11
+ -
+- 0
+ -
+- 0
8
refracpia.sfwdat : Page 1
inter 7
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
refracpia.sfwdat : Page 2
inter6.5
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
refracpia.sfwdat : Page 13
inter1
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Refractory period
Stimulus interval 7 ms
Amplitude are the same
Stimulus interval 6.5 ms
Smaller 2nd action potential No 2nd action potential
Stimulus interval 1 ms
เร่ิมเขาสู Relative RP เร่ิมเขาสู Absolute RP
Total RP = 7 msAbsolute RP = 1 ms
12
Refractory period
Total RP
When stimulus interval is 1 ms
When stimulus interval is 7 ms
Absolute RP
Relative RP = Total RP-Absolute RP
Relative RP
13
9
Anodal blockเมื่อกลับขั้วกระตุนโดยใชความแรงของตัวกระตุนเทาเดมิ
กอนกลับขั้วกระตุน หลังกลับขั้วกระตุน
• Amplitude ลดลง• Latency ยาวข้ึน• Stimulus artifact กลับหัว
กอน
หลัง
สังเกตุ: action potential ไมกลับหัว
14
Monophasic action potentialหลัง crush nerve ระหวางขั้วบันทึกที่ 4 และ 5
Biphasic action potential Monophasic action potential
15
10
Zoom of block pia.sfwdat : Page 1
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Zoom of block pia.sfwdat : Page 2
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Zoom of block pia.sfwdat : Page 6
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Xylocaineหยดระหวางขั้วกระตุนและขั้วบันทึกที่ 4
กอนหยด xylocaine หลังหยด xylocaine
16
Exp3_Napaporn
.85 0.9 0.95 1 1.05 1.1 1.15 1.2 1.25 1.3 1.35 1.4 1.45 1.5 1.55 1.
7/6/2549 11:39:27.765
Character of skeletal muscle contraction
stimulus
Compound muscle action potential
Muscle contraction
17
11
Threshold of muscle contraction(sciatic nerve stimulation)
400 mV 500 mVThresholdstimulus
600 mV 700 mV 800 mV 900 mVMaximalstimulus
1000 mV 1100 mV
Muscle contraction
Compound muscle action potential
Stimulus
18
L-T relationship -supornpim
Mus
cle
cont
ract
ion
(Ten
sion
) ...
0
20
40
60
1 2 3 4 5 6 7 8
EMG
(m
V)
-0.5
0.0
0.5
1.0
Mar
ker
(V)
-4
-2
0
2
4
5 0 0 0 0 0 0 5 03 4 5 6 7 8 9 10
6/6/2549 10:51:39.968 6/6/2549 10:52:09.359 6/6/2549 10:52:35.453 6/6/2549 10:53:50.562 6/6/2549 10:54:18.531 6/6/2549 10:54:34.671 6/6/2549 10:57:59.515 6/6/2549 10:58:23.906
Length-tension relationship
length +1 unit +2 unit +3 unit +4 unit +5 unit +6 unit +7 unit +8 unit
19
12
Chart WindowM
uscl
e co
ntra
ctio
n (T
ensi
on)
(...
0
10
20
30
40
50
60
EMG
(µV
)
-1000
-500
0
500
Mar
ker
(V)
-4
-2
0
2
4
1 2 0 1 0 1 0 1 0 1 0 1 0
7/6/2549 11:10:47.546
Summation of muscle contraction
Stimulusinterval 600 ms 400 ms 200 ms 100 ms 80 ms 60 ms 40 ms
20
total lab
Mus
cle
cont
ract
ion
(Ten
sion
) ...
0
20
40
60
80
5 H
z
10 H
z
20 H
z
30 H
z
40 H
z
EMG
(m
V)
-4
-2
0
2
4
Mar
ker
(V)
-4
-2
0
2
4
2 4 0 2 4 0 2 4 0 2 4 0 2 446 47 48 49 50
8/6/2549 15:52:16.015 8/6/2549 15:52:52.984 8/6/2549 15:53:56.671 8/6/2549 15:54:49.421 8/6/2549 15:55:35.546
Tetanic contractionIncomplete tetanic contraction Complete tetanic contraction
21
13
total lab
Mus
cle
cont
ract
ion
(Ten
sio.
..
0
20
40
60
80EM
G (
mV)
-4
-2
0
2
4
Mar
ker
(V)
-4
-2
0
2
4
1 1.5 2 2.5 3 3.5 4 4.5
8/6/2549 15:59:14.500
Muscle fatigue
Contraction = 1/3 of peak contraction
22
Exp4_Smooth Muscle_Akkradate
Chan
nel 1
(m
V)
-2
0
2
4
6
Ach,
3
7
ACh,
+3
35:00 36:40 38:20 40:00 41:40 43:20 45:00 46:40 48:20
16/6/2549 13:05:19.484
Smooth muscle contractionบทบาทของ Acetylcholine (ACh)
control ACh 3 drops Add Ach 3 drops
23
14
E4-R301-Example
Chan
nel 2
(m
V)
-8
-6
-4
-2
0
2
40:00 40:50 41:40 42:30 43:20 44:10 45:50 46:40 47:30
15/6/2549 10:23:24.578 15/6/2549 11:08:37.078
Smooth muscle contractionบทบาทของ Adrenaline (E)
control E 3 drops Wash
24
E4-R301-Example
Chan
nel 1
(m
V)
-5
0
5
1:10:00 1:10:50 1:11:40 1:12:30 1:13:20 1:14:10 1:15:00 1:15:50 1:16:40 1:17:30 1:18:20 1:19:10
15/6/2549 11:08:37.078
control Cold ringer
Smooth muscle contractionบทบาทของอุณหภมูิ
25
15
Exp.4 Smooth muscle 16-6-06
Chan
nel 1
(m
V)
-4
-2
0
2
4
16:40 17:30 18:20 19:10 20:50 21:40 22:30 23:20 24:10 25:00 25:50 26:40 27:30
16/6/2549 14:23:34.656 16/6/2549 14:43:44.656
control Ca2+ free Add Ca2+
Smooth muscle contractionบทบาทของ extracellular Ca2+
26
E4-R301-Example
Chan
nel 2
(m
V)
-8
-6
-4
-2
0
20
0.5%
KCl 3
dr
46:40 47:30 48:20 49:10 50:00 50:50 51:40 52:30
15/6/2549 11:08:37.078
control Add KCl
Smooth muscle contractionบทบาทของ extracellular K+
27
16
E4-R301-Example
Chan
nel 2
(m
V)
-5
0
5
24
0.5%
BaCl
2 2
dr
55:50 56:40 57:30 58:20 59:10 1:00:00 1:00:50
15/6/2549 11:08:37.078
control Add BaCl2
Smooth muscle contractionบทบาทของ extracellular Ba2+
28