third international conference of chemistryand...

Egypt. J.Chem. & Environ.Health

ThirdInternationalConferenceof

ChemistryAndEnvironmental

Health

10-14thNov.2016

ConferencePresident

Prof .Dr.MomtazShaheen

Director,AnimalHealthResearchInstitute

VicePresidentoftheConference

Prof .Dr.EssamIbraheemProf .Dr. OsamaEltaweel

ConferenceGeneralSecretary

Prof .Dr. KhaledElekhnawyProf .Dr.EmanElsaeed

ConferenceRapporteur

Prof .Dr.HananMohamedSobhy

HeadofBiochem.,Toxicol.&FeedDeff.Dep.

2016

ConferenceScientificCommittee

)AlphabeticalList(

.Prof .DrJobDescription AdelfayekfareedBacter..,AHRI

AhmedAbo-ElelaPhysiology.,BaniSweifuniv

AzzaMohamedKamalBioch.,AHRI

EmadMokhtarRiadBacter.,.AHRI

EmadRizkallahZakiBuffalodisease.AHRI

EmanBastawesyVirology,AHRI

EmanLazBioch.,AHRI

EssamIbraheem Patholo,AHRI

EssamHosnyRizkallahBioch.,AHRI

GehadRamadanChem.,Mansourauniv.

GehanGamilAhmedPathology,AHRI

GihanMohammedElmoghazyFoodSafety,AHRI

HananMohamedSobhyBioch.,.AHRI

HananTagEldin Bioch.,AHRI

HanyHelmyHaleemBioch.,AHRI

Harrell E. HurstPharma.andtoxicol.,

Louisvilleuniv. USA

Jakeen Kamal AbdelhaleemMicrobiology ,Vet. Med

Cairo Univ.

KhaledElekhnawyBioch.,AHRI

KhaledIbrahemFoodhygiene,

KhaledShawkyAlyFoodhygiene,AHRI

MahaElKholy Bioch.,AHRI

MahmoudElsayedParasitology,AHRI

MahmoudMohamedArafaBioch.,AHRI

Prof .Dr.JobDescription

MostafaFayezPharma.,Suezcanal,Uni

MostafaShalabyPharmaco.Vet.Med

,CairoUniv.

MogedaKamelMahmoudBioch.,.AHRI

MohamedGalalAggourBiotechnology,AHRI

MohamedKamalAbuElmagdBioch.,AHRI

MohamedKhaledElmossalamiFoodHygiene,

AssiutUniv.

NashaatAbdelMotaalFishdisease,AHRI

OsamaElTawilToxicol.,Cairouniv.

RamadanMostafaSalemMycology,AHRI

RaoufHelmyYousefBioch.,AHRI

SamiaAbedelazizElzeiniBioch.,AHRI

SoadAbdelAzizNLQP

Steven R. MyersPharma.andtoxicology,

Louisville univ.USA

SabryAhmedEl-khoderyMedicine&infectious diseases, Vet.

Med

Mansoura,Univ.

TawfikAboellailMicrobiol.,immunol.and

pathol.,Cloradouniv.USA

ViolaHassanZakiFishdiseaseDep.Mansoura

univ.

CONTENTS

Pages

6-18SESSION1

SESSION2 20 31-

33-39SESSION3

41-49SESSION4

51-59SESSION5

61-65SESSION6

Egyptian Journal Of Chemistry &

Environmental health

The First Session

للتدریب بالمعھد

Name (Prof. Dr.)

Jonathan

Freedman

Osama El Tawil

Ahmed Abo-Elela Hany Helmy

Mohamed Kamal

1- Study on the use of QuEChERS method for analysis of

organochlorine pesticides residues in cow's milk.

2- Detection of Ciguatoxin in canned fishes

3- Biological Age

Nutritional Status across Healthy Population Residing in

Jeddah between Ages 19 to 65

4- Magnetic Field Effects on Some Physiological Aspects of

Male Albino Rats

5- Hepatic ultrasonography and biochemical alterations in

Barki sheep under negative energy balance.

6- Hepatotoxicity And Nephrotoxicity Evaluation After

Repeated Dose of Acetamiprid In Albino Rats.

7- Removal of Atrazine herbicide from contaminated water

using molecularly imprinted nano

8- Variable chan

some endocrine disrupting compound affecting thyroid

hormones in Nile Catfish (

9-Study the incidence of Diabetes mellitus Type 1among

children in primary school age

10-Histopathological

albino rats injected with diethylnitrosamine and treated

with Camel’s milk and Curcuma longa.

Egyptian Journal Of Third Int. Conference OfChemistry & Chemistry &

health Environmental health

5

ession Is In The Large Training Hall, AHRI

للتدریب بالمعھد الكبرىالجلسة األولى بالقاعة

Number of

researches

Job description

1

Professor Professor Pharma.

and toxicology dep. Louisville

univ

Professor Toxicol. dep. Cairo univ. Professor Pharma. and

toxicology dep. Louisville univ. Physiology dep. Bani Sweif univ Elela

Bioch. dep. AHRI

Bioch. dep. AHRI Mohamed Kamal

Title Study on the use of QuEChERS method for analysis of

organochlorine pesticides residues in cow's milk.

Detection of Ciguatoxin in canned fishes.

Biological Age Associated with socioeconomic &


Jeddah between Ages 19 to 65.

Magnetic Field Effects on Some Physiological Aspects of

Male Albino Rats.

Hepatic ultrasonography and biochemical alterations in

rki sheep under negative energy balance.

Hepatotoxicity And Nephrotoxicity Evaluation After

Repeated Dose of Acetamiprid In Albino Rats.

Removal of Atrazine herbicide from contaminated water

using molecularly imprinted nano-polymer.

Variable change of environmental and climatic factors on


hormones in Nile Catfish (Clarias Gariepinus ).

Study the incidence of Diabetes mellitus Type 1among


Histopathological and biochemical studies in male Wistar


with Camel’s milk and Curcuma longa.

Conference Of &

Environmental health 2016

all, AHRI

Number of

researches 10

Page

6 7 9 11 12 14 15 16 17

18

Study on the use of QuEChERS method for analysis of

Associated with socioeconomic &


Magnetic Field Effects on Some Physiological Aspects of

Hepatic ultrasonography and biochemical alterations in

Hepatotoxicity And Nephrotoxicity Evaluation After

Removal of Atrazine herbicide from contaminated water

ge of environmental and climatic factors on


Study the incidence of Diabetes mellitus Type 1among

and biochemical studies in male Wistar




Study On TheU

Organochlorine

Sanya T. El Ghamry

1- Researcher of Food Hygiene at Mansura Vet. Lab, Animal Health Research Institute (AHRI),

Agricultural Research Center (ARC), Giza, Egypt.

2- Senior Researcher of Biochemistry at Damietta Sea' Port Lab, Animal Health Research

Institute (AHRI), Agricultural Research Center (ARC), Giza, Egypt.

QuEChERS stands for (Quick, Easy, Cheap, Effective, Rugged and Safe)

and pronounced as (catchers) is a method that had been developed for multi

pesticide residue analyses in food.

This study was conducted to investigate the efficacy of QuEChERS method

for analyzing the organochlorine pesticides residues in cow's milk.

Twenty organochlorine pesticide namely (Benzene, 1,3,5

Tetrachlorobenzene, Benzene pentachloro. alfa

Beta Lindane, Gammexane, Quintozene, Heptachlor, Aldrine, Heptachlor

epoxide, Endosulfan-I , p,p'

o,p' DDT , p,p'-DDT and Methoxychlor were examined.

The linearity of the standard curve (r

than (0.99) even with those compounds that showed low consistent recovery

rates (18-60%) such as 1,3,5

pentachloro- and Benzene, hexachloro

(25-1000ppb). The relative standard deviations of all compounds were less than

20% when using both AOAC, 2007.01(2007) and EN 15662 (2008) method.

It could be concluded that when analyzing pesticide residues (specially

organochlorine) in food (specially milk) using QuECHERS technique, spiked

blank samples with the compound or compounds under investigation should

presented in the run sequence in between the real examined samples to make

sure about the recovery rate of each analyte at different concentration to give an

exact interpretation of the results. Is important to develop and optimize more

specific methods when analyzing poorly recovered organochlorine pesticide in

milk.



6

Use Of QuEChERS Method For Analysis

rganochlorine Pesticides Residues In Cow's M

Sanya T. El Ghamry1 and Bazalou M.S.2

Researcher of Food Hygiene at Mansura Vet. Lab, Animal Health Research Institute (AHRI),

Agricultural Research Center (ARC), Giza, Egypt.

Senior Researcher of Biochemistry at Damietta Sea' Port Lab, Animal Health Research


ABSTRACT


and pronounced as (catchers) is a method that had been developed for multi

pesticide residue analyses in food.


for analyzing the organochlorine pesticides residues in cow's milk.

Twenty organochlorine pesticide namely (Benzene, 1,3,5

Tetrachlorobenzene, Benzene pentachloro. alfa-Lindane, Benzene hexachloro,


I , p,p'-DDE, Dieldrin , Endrin , Endosulfan

DDT and Methoxychlor were examined.

The linearity of the standard curve (r2) of all the compounds were not less


60%) such as 1,3,5-trichloro, Tetrachlorobenzene, Benzene,

and Benzene, hexachloro-) along the studied concentration range

1000ppb). The relative standard deviations of all compounds were less than




blank samples with the compound or compounds under investigation should




when analyzing poorly recovered organochlorine pesticide in

Conference Of &


nalysis Of

Milk.

Researcher of Food Hygiene at Mansura Vet. Lab, Animal Health Research Institute (AHRI),

Senior Researcher of Biochemistry at Damietta Sea' Port Lab, Animal Health Research



and pronounced as (catchers) is a method that had been developed for multi-


Twenty organochlorine pesticide namely (Benzene, 1,3,5-trichloro,

dane, Benzene hexachloro,


DDE, Dieldrin , Endrin , Endosulfan-II , DDD ,

DDT and Methoxychlor were examined.

) of all the compounds were not less


trichloro, Tetrachlorobenzene, Benzene,

) along the studied concentration range

1000ppb). The relative standard deviations of all compounds were less than




blank samples with the compound or compounds under investigation should be




when analyzing poorly recovered organochlorine pesticide in



Detection Nashwa A.H.Ahmed

Biochemist,Toxicol.& feed Deff.

The ciguatoxins (CTXs) are a group of marine biotoxins, which are the cause of

a foodborne intoxication known as ciguatera fish poisoning (CFP). CFP is

associated with consumption of coral reef fish from tropical

waters in the Pacific, Indian Oceans and the Caribbean Sea. The objective of

this study is twofold: first, to detect ciguatoxin concentration in some common

canned fishes in Egyptian markets to compare it with food safety acceptable

limits, and second, to evaluate adequacy of commercially available enzyme

linked immunosorbent assay (ELISA) kits and mouse bioassay for ciguatoxin

food safety assessment. Canned fish samples (three brands of

two brands of tunaand only one brand of sardine), five samples of each brand

were purchased from different supermarkets in Giza governorate. Two types of

commercially available fish ciguatoxin

(ELISA) kits were used, GSC

methods (that for general sample & animal tissue as described by Gscience kit

manufacturer) and only tissue sample extraction method as described by Sunred

manufacturer were examined.Mouse Bioassay (MBA) to confirm

results obtained by immunoassay was performed on Mackerel canned fish

(Sunshine brand). Results of ciguatoxin concentrations showed that all tested

canned fishes, whatever method of extraction or the used kit, were exceeding

the acceptable limit of ciguatoxin in fish meal according to the food safety

limits. Obtained data showed insignificant difference between the two

extraction methods those described by Gscience where estimated by the same

kit in most tested samples. Though, the great signif

results obtained by the two commercially available ciguatoxin ELISA kits, is

questionable. This difference may be due to difference in fish tissue extraction

procedures and extractive solvents (organic solvents vs. PBS).MBA was

performed on samples of canned fish (Mackerel; Sunshine brand), negative

control and even positive controls were found negative; none of the mice died

within 24 hours, also none of them suffer from weight loss in 24 hours or any

other toxicity symptoms.

results obtained by using the commercially available ELISA kit in determining

the concentrations of ciguatoxins in canned fishes. Also, the mouse bioassay



7

Detection Of Ciguatoxin In Canned Fishes Nashwa A.H.Ahmed1,Hanan M.Sobhy1, Hanan A.Tageldin

Esam M.Ibrahim2 Biochemist,Toxicol.& feed Deff.Dept.1and Pathology Dept. 2, Animal Health

Institute,Dokki,Giza.

ABSTRACT



associated with consumption of coral reef fish from tropical and subtropical




and second, to evaluate adequacy of commercially available enzyme


food safety assessment. Canned fish samples (three brands of Mackerel fish



commercially available fish ciguatoxin enzyme-linked immunosorbent assay

kits were used, GSCIENCE kit and SunRed kit. Two extraction



manufacturer were examined.Mouse Bioassay (MBA) to confirm




it of ciguatoxin in fish meal according to the food safety



kit in most tested samples. Though, the great significant difference between the







other toxicity symptoms. Finally, the authors lacked of contentment to the



Conference Of &


Hanan A.Tageldin1 and

, Animal Health Research



and subtropical




and second, to evaluate adequacy of commercially available enzyme


Mackerel fish,



linked immunosorbent assay

IENCE kit and SunRed kit. Two extraction



manufacturer were examined.Mouse Bioassay (MBA) to confirm toxicity and




it of ciguatoxin in fish meal according to the food safety



icant difference between the







the authors lacked of contentment to the





was not inadequate to detect ratios permitted by t

Authors recommended not to ignore the examination of ciguatoxin

concentration in canned fishes and the urgent need to find appropriate, sensitive

and accurate method to detect ciguatoxin .



8

was not inadequate to detect ratios permitted by the food safety organizations.



and accurate method to detect ciguatoxin .

Conference Of &


he food safety organizations.





Biological Age Associated

Status Across Healthy Population Residing

Thanaa.A.Elkholy1;

1) AlAzhar University – Egypt

Medical Sciences, KingAbdul

Background: Our understanding of the mechanisms by which aging is

produced is still very limited. Nutrition status focus on the areas such as staying

fit from 19 ≥ 60; Work

of chronic illness; assessing the biol

weight reduction and minimizing risk for chronic illness. The aim of this study

to find the association between socioeconomic &Nutritional Status

measuring the biological age length (telemeter length) for healthy

residents in Jeddah across 19

Methods: A cross- sectional descriptive study was carried out by two parts of

questioner contain several section to assess nutritional status, and to calculate

Biological age for 115 (56.3%) female and 88 (

The mean age for female was (27) and for male was (29). Patients with any

disease diagnosed were excluded.

Results: The majority of our participants (70%) their ages were ranging from

19 to 30 years old followed by (30%) were f

More than half (53.7%) of our participants had high income level (from 6000 to

>10000SR / month). A positive correlation between Bio age and levels of

income as total sample and statistically significant at (P<0.05).But no

significant different among male and female and Bio age.

There was highly significant differences between age groups at (p <0.000***)

in weight, BMI, mid arm circumference, waist circumference, however

significant differences for waist to hip ratio & for

grip (left) was at (P<0.05*). The majority percentage of obesity distribution

between overweight, obesity class 1, 2 and 3 was (83.1%) among (31

group followed by (75.0%) for (51

(53.7%) for (19-30) year age group. Likewise as gender prevalence obesity was

higher in male than female (59 vs. 40%). Lipid profile: High level LDL

&CHOL in male than female with highly significant differences among (male



9

Biological Age Associated With Socioeconomic &Nutritional

cross Healthy Population Residing In Jeddah

Ages 19 to 65

Amani al-rajhi2; Bashayer Khouja2; Doa'a al

and Nada al-amoudi2

Egypt- Cairo, 2) Departments of Clinical Nutrition, Faculty of Applied

Medical Sciences, KingAbdul-Aziz University, Jeddah, Saudi Arabia.

ABSTRACT

Our understanding of the mechanisms by which aging is


≥ 60; Work-Life Balance; Genetic risk factors and risk calculation

of chronic illness; assessing the biological age against the chronological age;


to find the association between socioeconomic &Nutritional Status

measuring the biological age length (telemeter length) for healthy

residents in Jeddah across 19-65 age.

sectional descriptive study was carried out by two parts of


Biological age for 115 (56.3%) female and 88 (43.7 %) male were conducted.


disease diagnosed were excluded.

The majority of our participants (70%) their ages were ranging from

19 to 30 years old followed by (30%) were from 31 to 50 years and above.




gnificant different among male and female and Bio age.



significant differences for waist to hip ratio & for triceps skin fold and hand


between overweight, obesity class 1, 2 and 3 was (83.1%) among (31

group followed by (75.0%) for (51 – 65) age categorized and third position

30) year age group. Likewise as gender prevalence obesity was



Conference Of &


ocioeconomic &Nutritional

n Jeddah Between

; Doa'a al-youbi2

Nutrition, Faculty of Applied

Aziz University, Jeddah, Saudi Arabia.

Our understanding of the mechanisms by which aging is


Life Balance; Genetic risk factors and risk calculation

ogical age against the chronological age;


to find the association between socioeconomic &Nutritional Statuson

measuring the biological age length (telemeter length) for healthy population

sectional descriptive study was carried out by two parts of


43.7 %) male were conducted.


The majority of our participants (70%) their ages were ranging from

rom 31 to 50 years and above.






triceps skin fold and hand


between overweight, obesity class 1, 2 and 3 was (83.1%) among (31- 50) age

65) age categorized and third position

30) year age group. Likewise as gender prevalence obesity was





&female) in LDL at (P<0.003**

than female with statistically sig (p<0.000***).

Blood Constituents: Iron HGB, HCT and RBC blood levels in male participants

were higher than in female with highly statistically significant differences at

(p<0.000***). High mean levels of VB9 & B12 in female than male (

8.43 ± vs. 16.32 5.24± ) & (222.8 vs. 151.31) respectively. Level of iron in the

blood was higher in male than female (13.19

intake: The mean of total energy intake as % from RDA was (62.02 ± 23.3) for

male and (59.2 ± 34.6) for female. The consumption of female was more than

male in all food groups but still less than normal as guidelines of 2010 for

healthy diet intake. A highly differences between male and female and the total

participants in fat intake as percent of RDA (149% vs. 109% vs.127)

statistically highly significant differences at (P<0.000***). The mean of real

age for male population less than bio age

may be that the male exposure to life stress and bad behavior life style practices

more than female which the average of their biological age was less than real

age (Chronological age) (22.7±7.6 & 27.0 ±9.5) respective

Conclusion: A healthy balance diet may protect against endothelial cell

senescence, generating by decrease in intracellular oxidative stress, telomere

shortening and cellular apoptosis. All these mechanisms may be involved in an

increased lifespan and a lower incidence of the diseases associated with aging.

Our Results in this study indicate to the need of rising nutritional awareness

among adult males to consumption healthy diet, practices physical exercise

daily and to reduce fast foods.

Key words: Biological age,

status, socioeconomic status.



10

&female) in LDL at (P<0.003**).Low in HDL level (good cholesterol) in male

than female with statistically sig (p<0.000***).



000***). High mean levels of VB9 & B12 in female than male (

) & (222.8 vs. 151.31) respectively. Level of iron in the

blood was higher in male than female (13.19 ±20.99 & 12.69 ±6.89). Food




ntake. A highly differences between male and female and the total



age for male population less than bio age (29.5 ±10.2 & 31.0±12.0) respectively



age (Chronological age) (22.7±7.6 & 27.0 ±9.5) respectively.

A healthy balance diet may protect against endothelial cell



a lower incidence of the diseases associated with aging.



daily and to reduce fast foods.

: Biological age, telemeter length, Chronological age, Nutritional

status, socioeconomic status.

Conference Of &


).Low in HDL level (good cholesterol) in male



000***). High mean levels of VB9 & B12 in female than male ( 22.4

) & (222.8 vs. 151.31) respectively. Level of iron in the

6.89). Food




ntake. A highly differences between male and female and the total



(29.5 ±10.2 & 31.0±12.0) respectively



A healthy balance diet may protect against endothelial cell



a lower incidence of the diseases associated with aging.



Chronological age, Nutritional



Magnetic Field Effects on Some Physiological Aspects of Male

Safaa, M. Abo El-Soud

1-Chemistry Department, Animal Health Research Institute, Dokki, Giza.

2-Plant protection Research Institute , Dokki, Giza.

3-Faculty of Engineering, Menofiya University.

The clear effects of SMF (static magnetic field) on many organs of the

body have not been known until now and have puzzled scientists. The main

goal of this study was toevaluate the possible effects of the whole

magnetic field exposure on the levels of some reproductive and thyroid

hormones (estradiol,testosterone

immunoglobulins (IgG and IgM) of male rats. Twenty mature male albino

rats with an average weight of 150 to 200g, randomly distributed to two groups

of 10 animals each.The first group was considered as the control group. The

second group was exposed to SMF of 20

days.The results showed a highly

testosterone but, estradiol, FSH, LH, T3 and T4 concentrations

significantly decreased

control group. This paralleled with a significant decrease (P

IgM serum levels. In conclusion, this study highlights some adverse effect of

SMF on some reproductive and thyroid hormones as well as some

immunoglobulins.



11


Albino Rats Soud1; Hussein, A.M2.; Rady,K.E3. and Allam, S.A

Department, Animal Health Research Institute, Dokki, Giza.

Plant protection Research Institute , Dokki, Giza.

Faculty of Engineering, Menofiya University.

ABSTRACT


been known until now and have puzzled scientists. The main

goal of this study was toevaluate the possible effects of the whole


testosterone, FSH, LH, T3 and T4)




xposed to SMF of 20-25 mT (millitesla) 24h/day for 10

days.The results showed a highly significant increase in the concentration of

testosterone but, estradiol, FSH, LH, T3 and T4 concentrations

decreased (P≤0.001) using this MF intensity in comparison to the

control group. This paralleled with a significant decrease (P≤0.01) in IgG and



Conference Of &



. and Allam, S.A2.


been known until now and have puzzled scientists. The main

goal of this study was toevaluate the possible effects of the whole-body


and some




25 mT (millitesla) 24h/day for 10

increase in the concentration of

testosterone but, estradiol, FSH, LH, T3 and T4 concentrations were

in comparison to the

≤0.01) in IgG and





Hepatic Ultrasonography

Sheep

Mohamed A. Youssef

Sayed**;Mohamed E. Awad*Department of Internal Medicine and Infectious

Mansoura University, Mansoura 35516, Egypt.**Department of Animal health and Poultry, Desert Research Center (DRC), Matariya, Cairo,

.***Biochemistry, Nutritional Diseases and Toxicology Department, Animal

As little is known about the ultrasonographic features of pregnancy

toxemia in sheep, the present study was designed to evaluate the significance of

ultrasound as well as markers of negative en

pregnancy toxemia in Egyptian Barki sheep. A total of seven apparently healthy

Barki sheep, in late stage of pregnancy, were subjected to daily clinical

examination. Among all ewes, only six animals completed the study. Th

selected ewes were exposed to

induce a state of negative energy balance. Serum and urine samples were

collected on the day before

five days. The collected serum samples were analyzed for glucose, cholesterol,

triglyceride, beta-hydroxyl butyrate (BHBA), non

(NEFA), insulin and leptin. Urine samples were also tested for the p

ketone bodies using reagent strips. In parallel, 5

transducer were used to examine the localization, visible extent of various

dimensions and appearance of the liver, portal vein, caudal vena cava and gall

bladder at different time points of the study. Our finding demonstrated that

ewes showed no detectable clinical findings throughout the study period.

Additionally, the biochemical analyses showed significant decrease in serum

values of glucose and cholesterol with a si

of triglyceride, BHBA, NEFA and insulin, while values of leptin showed no

significant difference throughout the different time points. Ketone bodies were

also negative in the urine of all examined animals throughout st

Ultrasonographic findings showed a significant increase (P

liver size at the 3rd day of

while the highest value was at the 5

other ultrasonographic liver measurements did not show any significant

variations. The diameter of portal vein and caudal vena cava were significantly

decreased (P˂ 0.05)at the 3



12

ltrasonography And Biochemical Alterations

heep Under Negative Energy Balance

Mohamed A. Youssef*;MagedR. El-Ashker *; Ahmed A. El

Sayed**;Mohamed E. Awad ***; Fathi A. Ibrahim **

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine,

Mansoura University, Mansoura 35516, Egypt.

Department of Animal health and Poultry, Desert Research Center (DRC), Matariya, Cairo,

Egypt

Biochemistry, Nutritional Diseases and Toxicology Department, Animal health Research

Institute (AHRI), Dokki, Giza, Egypt.

ABSTRACT



ultrasound as well as markers of negative energy balance for the diagnosis of



examination. Among all ewes, only six animals completed the study. Th

selected ewes were exposed to feed-restriction for five consecutive days to


collected on the day before feed-restriction (day 0) and then daily for the next


hydroxyl butyrate (BHBA), non-esterified fatty acids

(NEFA), insulin and leptin. Urine samples were also tested for the p

ketone bodies using reagent strips. In parallel, 5-MHz linear and convex



ent time points of the study. Our finding demonstrated that



values of glucose and cholesterol with a significant increase (p˂0.05) in values



also negative in the urine of all examined animals throughout st

Ultrasonographic findings showed a significant increase (P˂ 0.05) in the mean

day of feed-restriction and onward as compared with day 0,

while the highest value was at the 5th day of feed-restriction. However, the

trasonographic liver measurements did not show any significant


˂ 0.05)at the 3rd day of feed-restriction and ongoing as compared

Conference Of &


lterations In Barki

; Ahmed A. El-

Diseases, Faculty of Veterinary Medicine,

Department of Animal health and Poultry, Desert Research Center (DRC), Matariya, Cairo,

health Research



ergy balance for the diagnosis of



examination. Among all ewes, only six animals completed the study. The

for five consecutive days to


(day 0) and then daily for the next


esterified fatty acids

(NEFA), insulin and leptin. Urine samples were also tested for the presence of

MHz linear and convex



ent time points of the study. Our finding demonstrated that all



˂0.05) in values



also negative in the urine of all examined animals throughout study period.

˂ 0.05) in the mean

and onward as compared with day 0,

. However, the

trasonographic liver measurements did not show any significant


and ongoing as compared



with day 0, while the lowest valu

restriction. There were also a focal hyper echogenic areas in the liver

parenchyma observed at the 5

ultrasonography as well as metabolic profile could be used as an ad

for the diagnosis of negative energy balance in Barki sheep.



13

with day 0, while the lowest value observed was at the 5thday of

. There were also a focal hyper echogenic areas in the liver

parenchyma observed at the 5thday of feed-restriction. It can be concluded that

ultrasonography as well as metabolic profile could be used as an adjunctive tool

for the diagnosis of negative energy balance in Barki sheep.

Conference Of &


day of feed-

. There were also a focal hyper echogenic areas in the liver

. It can be concluded that

junctive tool



Hepatotoxicity And Nephrotoxicity Evaluation After Repeated Dose of Acetamiprid In Albino Rats

Mohamed A. Noaishi*, and H. H. Abd alhafez * Mammalian Toxicology Department,

Agricultural Research Center, Ministry of Agriculture, Egypt.

Acetamiprid (ACP) is neonicotinoid insecticide and it is the most highly

effective insecticides worldwide for crop protection and control fleas infesting

in livestock and pet animals. It has potential toxicity to mammals and there is

no clear data regards to hepatotoxicity, nephrotoxicity and oxidative stress at

low doses. The present study aimed to investigate the acute toxicity of ACP as

formulation toward male adult albino rats and evaluate the impact effect of

repeated sub-lethal dose of ACP. The

and the low dose approximately equivalent 1.5X of No Observed Adversed

Effect Level (NOAEL) dose. The study concluded the examination of

hepatotoxicity, nephrotoxicity and some parameters that covered oxidative

stress. The results revealed that, ACP may not have such extensive effects as

inducer to oxidative stress but may be induce synergism of the defense system

to increase the antioxidant self

investigation revealed that the e

and severe effects on the kidney tissue but may be has moderate effects on the

liver tissue. The low dose which equal approximately 1.5X of NOAEL led to

slight effects on the liver tissue while the kidney tiss

by this insecticide. Finally, It seems that the ACP induces the adverse effect at

the high dose level but the low dose level and short term exposure may not

make adverse effect. So,

using the recommended dose

Key Words: Acetamiprid, hepatotoxicity, nephrotoxicity, oxidative stress,

histopathology.



14

Hepatotoxicity And Nephrotoxicity Evaluation After Repeated

Dose of Acetamiprid In Albino Rats

Mohamed A. Noaishi*, and H. H. Abd alhafez Mammalian Toxicology Department, Central Agricultural Pesticides Lab. (CAPL),

Agricultural Research Center, Ministry of Agriculture, Egypt.

ABSTRACT




gards to hepatotoxicity, nephrotoxicity and oxidative stress at



lethal dose of ACP. The high dose equal 1/10 of estimated LD50




. The results revealed that, ACP may not have such extensive effects as


to increase the antioxidant self-system. Moreover, the histopathological

investigation revealed that the exposure to high dose of ACP led to destructive



slight effects on the liver tissue while the kidney tissue was still more affected



make adverse effect. So, it can be recommend to use this insecticide as

using the recommended dose.

Acetamiprid, hepatotoxicity, nephrotoxicity, oxidative stress,

Conference Of &


Hepatotoxicity And Nephrotoxicity Evaluation After Repeated

Central Agricultural Pesticides Lab. (CAPL),




gards to hepatotoxicity, nephrotoxicity and oxidative stress at



high dose equal 1/10 of estimated LD50




. The results revealed that, ACP may not have such extensive effects as


system. Moreover, the histopathological

xposure to high dose of ACP led to destructive



ue was still more affected



it can be recommend to use this insecticide as long as

Acetamiprid, hepatotoxicity, nephrotoxicity, oxidative stress,



Removal Of Atrazine

Using M

Mostafa Mosalam

1 Faculty of Science, Cairo

In the present work, molecularly polymeric nano

synthesized by miniemulsion polymerization procedure using Atrazine (ATZ)

as imprinting molecule. Three different monomers and two different cross

linkers were investigated. The MIPs obtained

in detail with rebinding investigation by HPLC

that the polarity of both monomer and cross

affinity and retention behavior to ATZ. The affinities of the polyme

increased with the decreasing polarity of cross

monomer is much more important than the cross

hydrophobic interaction and

a key role in the release process. This work focuses on synthesis of nano

particles and application of toxic contaminant material in water purification and

using miniemulition polymerization method. Molecularly imprinted

nanospheres (nanoMIPs) adsorbent for micropollutants

non-covalent templating technique. MIP nanoparticles has particle size about

140 nm were synthesized in toluene by using ATZ as a template, Itaconic acid

(ITA) as functional group, and EDMA as the cross

efficiency of the non-covalent molecular imprinting was examined by rebinding

experiments and quantified by HPLC

imprinted polymer, the MIP shows an excellent affinity towards ATZ in the

aqueous solution with imprinted factor (IF) 4.15.

Key words: Molecularly imprinted polymer (MIP), Atrazine (ATZ), Nano

polymer



15

f Atrazine Herbicide FromContaminated

Molecularly Imprinted Nano-polymer

Mostafa Mosalam1* and Johan Billing 2

Faculty of Science, Cairo University, Giza, Egypt, 2 MIP Technologies AB, Lund, Sweden

ABSTRACT

In the present work, molecularly polymeric nanoparticles were


as imprinting molecule. Three different monomers and two different cross

linkers were investigated. The MIPs obtained were subsequently characterized

in detail with rebinding investigation by HPLC-DAD UV. The results indicated

that the polarity of both monomer and cross-linker play an important role for

affinity and retention behavior to ATZ. The affinities of the polyme

increased with the decreasing polarity of cross-linker. It is illustrated that the

monomer is much more important than the cross-linker, and that the

hydrophobic interaction and π -π interaction between analytes and the MIP play

release process. This work focuses on synthesis of nano



nanospheres (nanoMIPs) adsorbent for micropollutants were prepared using

covalent templating technique. MIP nanoparticles has particle size about


(ITA) as functional group, and EDMA as the cross-linker polymer. The

covalent molecular imprinting was examined by rebinding

experiments and quantified by HPLC-UV. When compared with the non


aqueous solution with imprinted factor (IF) 4.15.

: Molecularly imprinted polymer (MIP), Atrazine (ATZ), Nano

Conference Of &


ontaminated Water

MIP Technologies AB, Lund, Sweden

particles were


as imprinting molecule. Three different monomers and two different cross-

were subsequently characterized

DAD UV. The results indicated

linker play an important role for

affinity and retention behavior to ATZ. The affinities of the polymers to ATZ

linker. It is illustrated that the

linker, and that the

interaction between analytes and the MIP play

release process. This work focuses on synthesis of nano-



were prepared using

covalent templating technique. MIP nanoparticles has particle size about


linker polymer. The

covalent molecular imprinting was examined by rebinding

UV. When compared with the non-


: Molecularly imprinted polymer (MIP), Atrazine (ATZ), Nano-



Variable Change

Some Endocrine

Hormones

Ali ,M.A.Hassan1;Hanan ,A. Tag El 1- Faculty of Science ,Chemistry Department ,Al

2- Biochemistry ,Toxicol. & Feed Deff.

Global Climatic change is receiving particular attention because it is

thought to cause habitat destruction and may increase exposure of humans to

extreme temperatures, polluted air and water, extreme weather events and

endocrine disrupters (EDCs)a

give new insights on the effect of some environmental and climatic factors that

may affect the level of some heavy metals in the aquatic system and also the

possible impact effects of these EDCs on thyro

and female catfish (Clarias gariepinus

Nile around Banha city (Kalubia governorate) and Idku Lake (Behera

governorate).Fishing trips to the studied areas were carried out every three

months over a period of one year from (summer 2014

samples (for each sex) were collected each season from every fishing sites. Five

water samples from each locality were collected seasonally. The climatic

changes in both sites were recorde

and Lead ( Pb) ] were determined in water and muscle samples. Quantitative

determination of serum thyroid stimulating hormone (TSH); Triiodothyronine

(T3) and thyroxin (T4) as well as T

changes in both sites were significantly (P < 0.01) influenced by season and

locality. Three heavy metals were significantly (P < 0.01) affected with season

except Pb was not detected in water from the two sits. Muscle heavy metals of

both sexes from the two sites were significantly affected by season and

location. TSH concentrations

fish sexes collected from Banha but only differed at autumn and spring for

those from Idku Lake.

concentrations with location all over the year except at spring for males and at

winter for females. T3

between the two sexes at summer, autumn,

collected from River Nile and Idku Lake, respectively. Male fish from River

Nile recorded lower T

significant lower ratio were present also at winter in fish from Idk

at summer, autumn and spring male fish have higher ratio than females.



16

hangeOf Environmental and Climatic Factors

ndocrine Disrupting Compound Affecting T

ormones In Nile Catfish (Clarias Gariepinus

;Hanan ,A. Tag El-Din2 ;Reda,A.A.Rezk2 ; Esam,H.,RizkallaFaculty of Science ,Chemistry Department ,Al-Azhar university, Cairo ,Egypt.

,Toxicol. & Feed Deff.Department, Animal Health Research Institute, Dokki,

ABSTRACT




endocrine disrupters (EDCs)as heavy metals. The aim of the present study is to



possible impact effects of these EDCs on thyroid hormones function in male

Clarias gariepinus).The study was conducted on the River



over a period of one year from (summer 2014–spring 2015). Ten fish



changes in both sites were recorded and heavy metals[ (Zinc (Zn) ;Copper (Cu)



) as well as T4/T3ratio were also determined. The climatic




oth sexes from the two sites were significantly affected by season and

TSH concentrations were significantly differed in all seasons for both


those from Idku Lake. Both sexes of fish showed significant variations in (


3 levels in the same site indicated significant variations

between the two sexes at summer, autumn, spring and at winter, spring in fish


T4/T3 ratio than females all over the year. The same

significant lower ratio were present also at winter in fish from Idku lake, while


Conference Of &


actors On

Thyroid

)

Rizkalla

esearch Institute, Dokki,




s heavy metals. The aim of the present study is to



id hormones function in male

).The study was conducted on the River



spring 2015). Ten fish



d and heavy metals[ (Zinc (Zn) ;Copper (Cu)



ined. The climatic




oth sexes from the two sites were significantly affected by season and

significantly differed in all seasons for both


sexes of fish showed significant variations in (T4)


levels in the same site indicated significant variations

spring and at winter, spring in fish


ratio than females all over the year. The same

u lake, while




Study TheIncidence

*Amany Mohamed Jasim

*Medical analysis Dept. College of

Feed Deff. Department, Animal Health Research Institute, Dokki, Giza.

The present study included 150 sample collected from children in primary

school age( male and female ) from (5

Karbala Hospital in Iraq .

their blood Glucose level


diabetes was also more frequently among who accidently acquired the disease

and those who had psychological pressure

diabetes and urinary tract infections and other diseases among diabetic children

studied

Key words : primary school



17

ncidence Of Diabetes Mellitus Type 1 A

Children In Primary School Age

Amany Mohamed Jasim and **Safaa ,S.Abd Al-Hamid

Medical analysis Dept. College of Medical &Health Technology&**Biochemistry ,Toxicol. &

Feed Deff. Department, Animal Health Research Institute, Dokki, Giza.

ABSTRACT


school age( male and female ) from (5-13) years attending laboratory of

Karbala Hospital in Iraq .all children blood samples were examined to estimate

level .Results suggested that the incidence of diabetic

children in primary school age not have a relation with age and gender ,


and those who had psychological pressure. There was correlation between


primary school, ketoacidosis, nutrition, Karbala, Glucose

Conference Of &


Among

Hamid

Biochemistry ,Toxicol. &


13) years attending laboratory of

all children blood samples were examined to estimate

diabetic

not have a relation with age and gender ,


was correlation between


Glucose



Histopathological Albino Rats Injected

With Camel’s

Fatimah, A.Al Homaid and Omaima , M.M. Hassan

Department of Biology,College of Science and Arts,Qassim University, Saudi Arabia

The purpose of this study is to evaluate the hepatoprotective effect of

Camel’s milk and the anti oxidant effect of Curcuma longa on

Diethylnitrosamine(DENA)induced hepatotoxicity in male Wistar albino rats.

Thirty rats fed on standard diet and

group received only tap water beside stander diet and consider as control group.

Second group was injected with a single intraperitoneal (i/p)dose of 1ml

DENA(60 mg/gm body weight).Third group was injected(i/p)

mg/gm b.wDENA and recived1ml of camel’s milk / each rat through stomach

tube as protective agent.Fourth group was injected(i/p) with 1ml of DENA

(60mg/gmb.w)and recived 2ml of camel’s milk /each rat.Fifth group was

injected (i/p) with 1ml of DENA 60mg/gm b.w and 1ml of Curcuma longa

extract /each rat and sixth group was injected(i/p) with 1ml of DENA (60

mg/gm b. w and 2ml of Curcuma longa extract / for each rat . This experamint

lasted for 120 day and the injection was daily .All a

and tap water during the experimental period (120 days). Blood and liver

samples were collected at the end of experiment for biochemical analysis and

histopathological investigation.The present finding revealed that(DENA)

increases the level of malondialdehyde (MDA) while it decreases the level of

antioxidants {glutathione(GSH),superoxid dismutase(SOD), catalase(CAT) and

glutathioneperoxidase(GSH

aminotransferase(AST),alanine aminot

transpeptidase (GGT), alkaline phosphatase(ALP) and lactate

ehydrogenase(LDH) }showed significant increase with (DENA)which exhibit

it’s bad effect on target cells (Hepatically). Moreover,Total proteins levels,

albumin,α1,α2 β1,β2,γ1 and γ2 showed significant decrease while A/G ratio

was insignificantly affected. Histopathological examination of the liver showed

that administration of( DEN) caused significant histological damage

represented by degeneration of hepatocytes ,f

hemorrhage. Also, our results revealed that both camel’s milk and Curcuma

longa have good roles in improving treated groups biochemicaly and

histopathologicaly. This may be due to their protective or antioxidant effects.



18

Histopathological And Biochemical Studies In Male Wistar

njected With Diethylnitrosamine And Tith Camel’s Milk And Curcuma longa.



ABSTRACT




Thirty rats fed on standard diet and tap water were divided into six groups. First



DENA(60 mg/gm body weight).Third group was injected(i/p) with 1ml of (60




1ml of DENA 60mg/gm b.w and 1ml of Curcuma longa



lasted for 120 day and the injection was daily .All animals fed on standard diet




ses the level of malondialdehyde (MDA) while it decreases the level of


glutathioneperoxidase(GSH-Px) }. Onthe other hand, all enzymes {aspartate

aminotransferase(AST),alanine aminotransferase(ALT),gamma glutamyl




β1,β2,γ1 and γ2 showed significant decrease while A/G ratio


administration of( DEN) caused significant histological damage

represented by degeneration of hepatocytes ,fibromawith sever congestion and

Also, our results revealed that both camel’s milk and Curcuma



Conference Of &


ale Wistar Treated






tap water were divided into six groups. First



with 1ml of (60




1ml of DENA 60mg/gm b.w and 1ml of Curcuma longa



nimals fed on standard diet




ses the level of malondialdehyde (MDA) while it decreases the level of


Px) }. Onthe other hand, all enzymes {aspartate

ransferase(ALT),gamma glutamyl




β1,β2,γ1 and γ2 showed significant decrease while A/G ratio


administration of( DEN) caused significant histological damage

ibromawith sever congestion and

Also, our results revealed that both camel’s milk and Curcuma





The SecondSession

مركز الدولى للزراعة

Name (Prof. Dr.)

Jill Steinbach

Emad Mokhtar Riad Jakeen El Jakee

Eman Bastawesy

1- Occurrence of plasmid

(PMABLs) resistance genes among

Isolated from bovine diarrhea

2- Immune response against salmonella infection in chicken

using cynarin as immunostimulant

3- Molecular Characterization And Hemato Biochemical

Studies Of Reovirus In Ismailia Farms

4- Some Bacteriological and Molecular Studies on

Escherichia Coli As Caustive Agent of Calves Enteritis at

Domyta Governorate.

5- Bacteriological and clinicopathological studies

listeriosisand detection of some virulence genes of

monocytogenes

6- Prevalence of mycobacterium in cattle milk and some

milk products in El sharkia Governorate

7- Effect of Probiotic on

broiler chickens

8- Evaluation of ELISA and the conventional methods in

diagnosis of JOHNE’ S Disease in cows

9-A STUDY ON BACTERIAL AND FUNGAL CAUSES

OF SUBCLINICAL MASTITIS IN DAIRY COWS

10- A Study On Bacterial And Fungal Causes Of Subclinical Mastitis In Dairy Cows



19

ession Is In TheMain Hall, International C

Agriculture

مركز الدولى للزراعةالبالجلسة الثانیة بالقاعة الكبرى

Number of researches

Job description

1

Professor of . Louisville Dep.Microbiology

univ

Jill Steinbach

Bacteriology dep. AHRI

Professor of Microbiology Fac. Vet. Med. Cairo Univ.

Virology dep. Professor of

AHRI Eman Bastawesy

Title

Occurrence of plasmid-mediated ampC β-lactamases

(PMABLs) resistance genes among E. coli O157:H7

Isolated from bovine diarrhea

Immune response against salmonella infection in chicken

using cynarin as immunostimulant

Characterization And Hemato Biochemical

Studies Of Reovirus In Ismailia Farms

Some Bacteriological and Molecular Studies on

Escherichia Coli As Caustive Agent of Calves Enteritis at

Domyta Governorate.

Bacteriological and clinicopathological studies on rabbit

listeriosisand detection of some virulence genes of L.

monocytogenes by PCR.

Prevalence of mycobacterium in cattle milk and some

milk products in El sharkia Governorate

Effect of Probiotic on SalmonellaEnteritidis infection on

kens

Evaluation of ELISA and the conventional methods in

diagnosis of JOHNE’ S Disease in cows

A STUDY ON BACTERIAL AND FUNGAL CAUSES

OF SUBCLINICAL MASTITIS IN DAIRY COWS

A Study On Bacterial And Fungal Causes Of Subclinical Mastitis In Dairy Cows

Conference Of &


Center for


01

Page

20 21 22 23 24 26 27

29

30

31



Occurrence of

(PMABLs) Resistance

Shaimaa, R.A.1; Gad El

1 Bacteriology Department, Animal Health Research Institute (AHRI);

Department, Faculty of Veterinary Medicine, Cairo University

5 isolates of E. coli

from bovine (100 calf`s fecal samples; 216 calf`s internal organs; 100 cow`s

fecal samples and 200 cow`s internal organs). The samples were submitted for

the isolation and identification of

examined for their susceptibility to 16 antimicrobial agents. Multiplex PCR was

used for the detection of plasmid

isolated strains.

E.coli O157:H7 isolated with rate of (2%) from the feca

and cows. While only o.46% were isolated from internal organs of calves,

meanwhile E.coli O157:H7 not isolated from cows samples . The isolates

showed multidrug resistance ranged from 6%

β lactamas genes were

isolates showed presence CMY

the presence of DHA-

MIR-1T and ACT-1 were detected at a of LAT

fragment 302bp.

PCR remains the gold standard for detection of plasmid AmpC

lactamases. This study may represent the first report of variants of MIR

ACT-1 �-lactamase-producing

not reflect the true number of PMABLs producers. Genotypic methods need to

be employed in national surveillance studies in Egypt.



20

Occurrence of Plasmid-mediated ampC β-lactamases

esistance Genes AmongE. coli O157:H7 Isolated

From Bovine Diarrhea

Gad El-Said, W.A. 2; Soumaya, S.A. El-Shafii1

Farag1

Bacteriology Department, Animal Health Research Institute (AHRI); 2 Microbiology

Department, Faculty of Veterinary Medicine, Cairo University

ABSTRACT

E. coli O157:H7 were isolated from 616 samples collected



the isolation and identification of E.coli O157:H7. The identified isolates were


used for the detection of plasmid-mediated ampC β-lactamases (MAABLs) in

O157:H7 isolated with rate of (2%) from the fecal sample of calves


O157:H7 not isolated from cows samples . The isolates

showed multidrug resistance ranged from 6%- 100%. AmpC plasmid mediated

β lactamas genes were detected in all isolates of E.coli O157:H7, where 3

isolates showed presence CMY-7, BIL-1 at fragment 462bp, one isolate showed

-1, DHA-2 at fragment 405bp and one isolate showed

1 were detected at a of LAT-1 to LAT-4, CM

PCR remains the gold standard for detection of plasmid AmpC

lactamases. This study may represent the first report of variants of MIR

producing E. coli in Egypt. Phenotypic methods alone may

true number of PMABLs producers. Genotypic methods need to

be employed in national surveillance studies in Egypt.

Conference Of &


lactamases

O157:H7 Isolated

1; Azza, N.

Microbiology

O157:H7 were isolated from 616 samples collected



he identified isolates were


lactamases (MAABLs) in

l sample of calves


O157:H7 not isolated from cows samples . The isolates

100%. AmpC plasmid mediated

O157:H7, where 3

1 at fragment 462bp, one isolate showed

2 at fragment 405bp and one isolate showed

4, CMY-2 to

PCR remains the gold standard for detection of plasmid AmpC �-

lactamases. This study may represent the first report of variants of MIR-1Tand

Phenotypic methods alone may

true number of PMABLs producers. Genotypic methods need to



Immune Response

Using

Manal, A. R.*; Hassan, W.H.**; Soumaya, S.A.El

* Animal Health Research Institute, Fayoum, **Department of Bacteriology, Mycology and

Immunology, faculty of veterinary medicine Beni

Resea

A total of 900 samples of internal organs (liver, spleen, intestine from

each bird) was aseptically collected from 300 diseased and apparently healthy

Hubbard and Ross broiler chickens of different ages

farms in El-Fayoum Governorate. The rate of isolation of

speciesfrom broilers of different ages was (5%), where 15 strains were isolated

from 300 broilers. Salmonellae were isolated from diseased and apparently

healthy broilers with an incidence of 9.7% and 0.6% respectively. The highest

rate of salmonella isola

followed by 3.5% in intestine of diseased broilers and only (0.6%) in intestine

of apparently healthy broilers, while salmonella species were not isolated from

spleen of both apparently healthy and dis

most predominant isolate (53.3%), followed by

S.Infantis (13.3%). S.Enteritidis

cefotaxime, lincomycin and sulfo

showed sensitivity to ciprofloxacin with a rate of 50%.

A total of 70 chicks was usedto evaluate the protective value of cynarin

against S.Enteritidis.The mean of optical density (MOD) of serum antibodies

IgG in groups treated with cynarin using indirect

It was concluded that none of the used antibiotics was 100 % effective;

on the other hand, multidrug resistance patterns have been recorded among all

isolates examined. Cynarin exhibits high antimicrobial activities against

S.Enteritidis, and could be used to reduce the usage of antibiotics and its

related side effect and highlights the importance of them as complementary

tools but not substitutes of integral biosecurity programs against the infection

in poultry flocks. Herbs effective

application on large scales.



21

esponse AgainstSalmonella Infection In C

sing Cynarin as Immunostimulant

Manal, A. R.*; Hassan, W.H.**; Soumaya, S.A.El-Shafii***; and

H. Salam** * Animal Health Research Institute, Fayoum, **Department of Bacteriology, Mycology and

Immunology, faculty of veterinary medicine Beni-Suef University, *** Animal Health

Research Institute, Bacteriology Department Dokki

ABSTRACT



Hubbard and Ross broiler chickens of different ages (3-5weeks) from different

Fayoum Governorate. The rate of isolation of




rate of salmonella isolation (6.9%) was recorded in liver of diseased broilers



both apparently healthy and diseased broilers. S.Enteritidis

most predominant isolate (53.3%), followed by S.Typhimurium (33.3%) and

S.Enteritidis isolates showed complete resistance to

cefotaxime, lincomycin and sulfo-methoxazole trimethoprim. S.Enteritidis

showed sensitivity to ciprofloxacin with a rate of 50%.


.The mean of optical density (MOD) of serum antibodies

IgG in groups treated with cynarin using indirect ELISA were observed.




, and could be used to reduce the usage of antibiotics and its



in poultry flocks. Herbs effectiveness on humeral response needs more

application on large scales.

Conference Of &


Chicken

Shafii***; and Hala S.

* Animal Health Research Institute, Fayoum, **Department of Bacteriology, Mycology and

Suef University, *** Animal Health



weeks) from different

Fayoum Governorate. The rate of isolation of Salmonella




tion (6.9%) was recorded in liver of diseased broilers



S.Enteritidis was the

(33.3%) and

isolates showed complete resistance to

S.Enteritidis


.The mean of optical density (MOD) of serum antibodies

ELISA were observed.




, and could be used to reduce the usage of antibiotics and its



ness on humeral response needs more



Molecular Characterization And Hemato Biochemical Studies

Of Reovirus In Ismailia Farms

Neven M. Ramzy

Department of Virology1, Department of Clinical

The avian reoviruse induces various manifestations in chickens. They

are associated with disease conditions including malabsorption syndrome and

tenosynovitis. RT-PCR is a rapid, sensitive and specific diagnostic test for

ARV detection and avoiding econo

Ismailia with reovirus like symptoms were screened for Reoviruses in SPF

embryonated chicken eggs. Three isolates of Reovirus were obtained and

characterized and identified by RT

specific band at 399 bp positive at lane 1, 3 and negative at lane 2.

Results of haematological investigation showed low levels of total erythrocytic

count (TEC), haemoglobin (Hb), and packed cell volume (PCV) but significant

increase in level of total leukocytic count (TLC), absolute heterophils count

(AHC), absolute lymphocyte co

in infected chickens compared to apparently healthy. Biochmical estimation

revealed marked elevation of total serum proteins, AST, ALT. Pathological

changes in internal organs of the infected chicks were also de

alterations were suggested to interfere with the immunity and normal digestive

processes resulting in poor weight gain and stunting of chicks. The

proventriculus revealed marked hyperplasia of lymphoid aggregates and the

small intestine revealed marked loss of villi with replacement by fibrin, necrotic

debris, high numbers of macrophages and lymphocytes .

Key words: Reovirus- PCR

Pathology



22


Of Reovirus In Ismailia Farms

Neven M. Ramzy1, Hala N. Ibrahim2, Seham F. ElHadad

, Department of Clinical pathology2, Department of Pathology

health research Institute, Doki, Egypt.

ABSTRACT



PCR is a rapid, sensitive and specific diagnostic test for

ARV detection and avoiding economic losses. Three broiler poultry farms in



characterized and identified by RT-PCR. Electrophoretic pattern show

specific band at 399 bp positive at lane 1, 3 and negative at lane 2.

of haematological investigation showed low levels of total erythrocytic



(AHC), absolute lymphocyte count (ALC) and absolute monocyte count (AMC)



changes in internal organs of the infected chicks were also described. All these




revealed marked loss of villi with replacement by fibrin, necrotic

debris, high numbers of macrophages and lymphocytes .

PCR- Sequence- chicken- hematobiochemical alteration

Conference Of &



, Seham F. ElHadad3

, Department of Pathology3Animal



PCR is a rapid, sensitive and specific diagnostic test for

mic losses. Three broiler poultry farms in



PCR. Electrophoretic pattern showed a

of haematological investigation showed low levels of total erythrocytic



unt (ALC) and absolute monocyte count (AMC)



scribed. All these




revealed marked loss of villi with replacement by fibrin, necrotic

hematobiochemical alteration-



Some Bacteriological and Molecular Studies

As Caustive Agent of Calves Enteritis at Domyta Governorate.

1Rehab E. Dawod,

1Animal Health Research Institute, Damietta Branch, Egypt2,3Animal Health Research Institute, Port

The present study carried out to evaluate the presence of diarrheagenic

Escherichia as causes of diarrhea in young calves in Damietta governorate

during period from November 2015 to March 2016. Bacteriological

examination was done on a total of

diarrhoeic calves of average age of 2 to 21 days to detect the prevalence of E.

coli . The samples were grouped according to the age of the calves into 3 age

groups as follows: 1st age group (2

group (15-21 dayes). The results revealed isolation of 27 strains of E.

(27%). Serotyping of the isolated strains according to somatic (O) and flagellar

(H) antigens detected the presence of 6 entertoxigenic E.

13 enterohemorrhagic E.

(EPEC; 18.52%) and 3 enterinvasive E.

PCR for the detection of E.

(Stx1; 88.89%), (Stx2; 51.85%), produced from Shiga toxin producing E. coli

(STEC); intimin (eaeA; 29.63%) and hylA (59.26%) genes was done. Each

isolate was found to carry one or more virulence genes. Mixed E.

is strongly associated with the cases of diarrhea

virulence genes in some isolates. Diarrheic calves were found in high

prevalence in the 1st age group (43%) whereas was found in prevalence in the

2nd group (33%) and in 3

Antibiogram study revealed that

sensitive to Ciprofloxacin and Cefotaxime, moderately sensitive to Gentamicin

and resistance to Nalidixic acid and Erythromycine. The present study suggests

the importance of maintain strict hygienic measures to p

pathogens to healthy calves.

Key Words: diarrheagenic E.



23

Some Bacteriological and Molecular Studies on Escherichia Coli


Rehab E. Dawod,2 Gihan M.O. Mohammed &3 Helal, I.M

Animal Health Research Institute, Damietta Branch, Egypt

Animal Health Research Institute, Port-Said Branch, Egypt

ABSTRACT




examination was done on a total of (100) fecal samples collected from young


. The samples were grouped according to the age of the calves into 3 age

age group (2-7 dayes), 2nd age group (8-14 dayes) and 3

21 dayes). The results revealed isolation of 27 strains of E.


(H) antigens detected the presence of 6 entertoxigenic E. coli (ETEC; 22.22%);

13 enterohemorrhagic E. coli (EHEC; 48.15%); 5 enteropathogenic E.

(EPEC; 18.52%) and 3 enterinvasive E. coli (EIEC; 11.11%). Application of

PCR for the detection of E. coli virulence genes including; two Shiga toxins:

x2; 51.85%), produced from Shiga toxin producing E. coli


isolate was found to carry one or more virulence genes. Mixed E. coli

is strongly associated with the cases of diarrhea due to the presence of several



group (33%) and in 3rd group (24%) .

Antibiogram study revealed that the isolated E. coli strains were highly



the importance of maintain strict hygienic measures to prevent spreading any

pathogens to healthy calves.

diarrheagenic E. coli, calves, Virulence genes, Antibiogram.

Conference Of &


on Escherichia Coli


Helal, I.M




(100) fecal samples collected from young


. The samples were grouped according to the age of the calves into 3 age

14 dayes) and 3rd

21 dayes). The results revealed isolation of 27 strains of E. coli


EC; 22.22%);

(EHEC; 48.15%); 5 enteropathogenic E. coli

(EIEC; 11.11%). Application of

virulence genes including; two Shiga toxins:

x2; 51.85%), produced from Shiga toxin producing E. coli


coli infection

due to the presence of several



strains were highly



revent spreading any

, calves, Virulence genes, Antibiogram.



Bacteriological A

ListeriosisAnd

Ghada A. Ibrahim

1 Researcher, Bacteriology department, AHRI, Ismailia branch, Bacteriology.&

clinical pathology department, AHRI, Ismailia branch, pathology.

Rabbit listeriosis is one of the major diseases problems that facing rabbit

breeding and industry in Egypt.

foodborne pathogen for listeriosis in humans, rabbits and many animal species.

The scope of the prese

characterization of some virulence genes (16s r

monocytogenes isolates in rabbits and to show the correlation between the

intensity of the experimental infection and hematological

and their effects on the immune status of the animal. Conventional

bacteriological examination for isolation of

all collected samples including culturing on specific media and biochemical

identification test. Samples were collected from suspected cases of rabbit farms

in Ismailia Governorate. Moreover, an oral experimental trial for induction of

listeriosis in two groups of rabbits (1

control) was also, done.

percentage of 21.8% (12/55) however; experimentally it was reisolated from all

rabbits of the 1st group. Microcytic hypochromic anaemia, leukocytosis

associated with neutrophilla, lymphocytosis and monocyto

experimentally infected rabbits at 7 days p.i. and leukocytosis with neutrophilia

in 15 days p.i. A highly significant elevation in the activities of ALT and AST,

hypocalcemia associated with significant increase in uric acid and inorg

phosphorus were observed in infected group. Meanwhile, highly significant

decrease in total proteins associated with decrease of total globulins especially

α and β-globulin and hypoalbuminemia were recorded. PCR analysis of

16S rRNA gene was applied f

of L. monocytogenes from other

all the recovered isolates (100%).

virulent genes with PCR revealed that

monocytogenes isolates (91.7%) meanwhile,

bp in all isolates (100%) confirming more virulence and pathogencity

oftheseisolates. Recommendation of successful preventive, good sanitation

programs



24

And Clinicopathological Studies On

ndDetection of Some Virulence Genes of

monocytogenesBy PCR.

Ghada A. Ibrahim 1 andHala N. Ibrahim2

Researcher, Bacteriology department, AHRI, Ismailia branch, Bacteriology.&2

clinical pathology department, AHRI, Ismailia branch, pathology.

ABSTRACT


breeding and industry in Egypt. Listeria monocytogenes is the main responsible


The scope of the present study was to discuss the phenotypic and genotypic

characterization of some virulence genes (16s rRNA, hlyA and

isolates in rabbits and to show the correlation between the

intensity of the experimental infection and hematological, biochemical changes


bacteriological examination for isolation of L. monocytogenes was applied for


n test. Samples were collected from suspected cases of rabbit farms


listeriosis in two groups of rabbits (1st was the infected group and 2

control) was also, done. L. monocytogenes was isolated from rabbit farms in a


group. Microcytic hypochromic anaemia, leukocytosis

associated with neutrophilla, lymphocytosis and monocytosis were observed in



hypocalcemia associated with significant increase in uric acid and inorg



globulin and hypoalbuminemia were recorded. PCR analysis of

rRNA gene was applied for the molecular identification and differentiation

monocytogenes from other Listeria species. 16S rRNA gene was found in

all the recovered isolates (100%). Also, the genotypic detection of some

virulent genes with PCR revealed that hly A gene was detected in (11/12) of

isolates (91.7%) meanwhile, iap gene gave clear bands at 193



Conference Of &


n Rabbit

enes of L.

2 Researcher,


is the main responsible


nt study was to discuss the phenotypic and genotypic

and iap) of L.

isolates in rabbits and to show the correlation between the

, biochemical changes


was applied for


n test. Samples were collected from suspected cases of rabbit farms


was the infected group and 2nd was the

was isolated from rabbit farms in a


group. Microcytic hypochromic anaemia, leukocytosis

sis were observed in



hypocalcemia associated with significant increase in uric acid and inorganic



globulin and hypoalbuminemia were recorded. PCR analysis of

or the molecular identification and differentiation

was found in

Also, the genotypic detection of some

tected in (11/12) of L.

gene gave clear bands at 193





and control measures in rabbit farms should be implemented.

vitro and in vivo studies for the role and mechanism of other virulence factors

of L. monocytogenes in rabbit in Egypt should be discussed and explained

prevent the spreading of l

the rabbit industry in Egypt.

Keywords: rabbit, isolation,

immunological, biochemical, haematological, phosphorus, anemia.



25

control measures in rabbit farms should be implemented. Also, further in


in rabbit in Egypt should be discussed and explained

prevent the spreading of listeriosis disease in rabbit and for the improvement of

the rabbit industry in Egypt.

rabbit, isolation, L.monocytogenes, 16S rRNA, gene, iap,

immunological, biochemical, haematological, phosphorus, anemia.

Conference Of &


Also, further in


in rabbit in Egypt should be discussed and explained to

for the improvement of

rRNA, gene, iap,



Prevalence of M

products

Saeid ,I.M., Riad,E.M.and Hassan,M.Gab

Animal Health Research Institute

The use of raw milk in the production of cheese and other dairy

products considered as potential public health risk associated with bovine

tuberculosis , as viable mycobacteria (including

survive in unpasteurized cheeses.

cows was regarded as the principal vehicle of infection for humans before the

advent of compulsory milk pasteurization.

A total of 459 samples were collected including 200 milk samples, 85

ice-cream samples ,97 kareish cheese samples and 77 yoghurt samples. 100

milk samples were collected from (3) private farms and 100 milk samples were

collected from street venders as

collected randomly from dairy farms and markets. All milk and milk products

samples were prepared and examined by conventional methods ( cultural and

microscopic procedures) as well as by using real time PCR

The conventional culture technique showed that, Out of 459 examined

milk and milk products samples, 12 samples were positive for

bovis with a percentage of 2.6% . Regarding to conventional culture technique on

raw milk samples, 5 out of 200 were positive to

percentage of isolation reached (2.5 %).

While (2) out of 97 kareish cheese samples were positive with

of (2%) and (2) samples out of 85 ice cream samples were positive with a

percentage of (2.3%) . While the results of PCR revealed( 8 ) of examined milk

samples were positive and ( 9) milk products samples were positive for

mycobacterium bovis using real time PCR.

Key words: Mycobacterium tuberculosis, Real time PCR, Conventional

method ,Milk and Milk Products.



26

Mycobacterium InCattle Milk and Some

products In El Sharkia Governorate

Saeid ,I.M., Riad,E.M.and Hassan,M.Gab-ALLa

Animal Health Research Institute –Dokki- Giza- Zagazeg branch

ABSTRACT



tuberculosis , as viable mycobacteria (including M. bovis) have been found to

survive in unpasteurized cheeses. Unpasteurized milk derived from infected


advent of compulsory milk pasteurization.


cream samples ,97 kareish cheese samples and 77 yoghurt samples. 100


collected from street venders as well as the milk products samples which



microscopic procedures) as well as by using real time PCR.


milk and milk products samples, 12 samples were positive for

with a percentage of 2.6% . Regarding to conventional culture technique on

raw milk samples, 5 out of 200 were positive to Mycobacterium bovis

percentage of isolation reached (2.5 %).

While (2) out of 97 kareish cheese samples were positive with




bovis using real time PCR.

: Mycobacterium tuberculosis, Real time PCR, Conventional

method ,Milk and Milk Products.

Conference Of &


ome Milk



) have been found to

Unpasteurized milk derived from infected



cream samples ,97 kareish cheese samples and 77 yoghurt samples. 100


well as the milk products samples which



.


milk and milk products samples, 12 samples were positive for Mycobacterium

with a percentage of 2.6% . Regarding to conventional culture technique on

Mycobacterium bovis with a

While (2) out of 97 kareish cheese samples were positive with a percentage




: Mycobacterium tuberculosis, Real time PCR, Conventional



Effect Of Probiotic

Dina, M.W. Shibat El

1 Department of Poultry Diseases, Animal Health Research Institute, Qena Laboratory,

Egypt.2Department of Bacteriology&Mycology, Faculty of Veterinary Medicine, South Valley

Probiotics is live cultures of microorganisms administered orally, acted

beneficially on host health. The addition of probiotics to the diet of poultry has

been found to improve growth performance, feed conversion efficiency,

immune responses and help in c

applied to determine the role of probiotics for preventing

infection and its effect on the performance as well as the immune response of

broiler chickens. The studied

previously,SalmonellaEnteritidis

Provence. One hundred and twenty, one day old Ross broiler chicks were

divided into four equal groups, first group (1) fed on a balanced ration and

considered the negativ

ration and provided with the probiotic

containing Lactobacillus Plantarum 1×

1×108cfu and Saccharomyces Cerevisiae

successive days, third group (3) was challenged with

Cfu / ml after Probiotics treatment and the fourth group (4) was challenged with

SalmonellaEnteritidis

control group. All groups were kept under complete observation for 4 weeks.

Throughout the time of the experiment, both clinical signs and post mortum

lesions were recorded for all groups, body weight (BW), food conversion rate

(FCR), hemogram, serum biochemical parameters and humoral immunity using

ELISA technique were investigated. Results revealed high performance

parameters as an increase in body weight and FCR. Neither clinical signs nor

PM appeared in both non infected group and the probiotics

infected, probiotics treated group showed mild decrease in the performance

parameters and mild degree of clinical sings and PM lesions for


showed significant decrease in body weight,low of the performance parameters

and characteristics sings and pm lesions for

along the experiment. Serum biochemical parameter showed elevated total

proteins and albumin in probiotic treated group

groups.Results of ELISA



27

f Probiotic On SalmonellaEnteritidisInfection

Broiler Chickens

Dina, M.W. Shibat El-hamd1 and Hams, M. Ahmed2

Department of Poultry Diseases, Animal Health Research Institute, Qena Laboratory,

Department of Bacteriology&Mycology, Faculty of Veterinary Medicine, South Valley

University




immune responses and help in combating enteric pathogens. So this study was

applied to determine the role of probiotics for preventing SalmonellaEnteritidis


The studied SalmonellaEnteritidis isolate studied

SalmonellaEnteritidis wasisolated from chickens from Qena



considered the negative control group, second group (2) fed on a balanced

ration and provided with the probiotic Micro- Procell (cheil- Bio.com. LTD)

containing Lactobacillus Plantarum 1×108cfu, Lactobacillus Acidophilus

cfu and Saccharomyces Cerevisiae 1×107cfu, in drinking water for 5

successive days, third group (3) was challenged with SalmonellaEnteritidis


109 Cfu / ml at 7day old and considered the p




um biochemical parameters and humoral immunity using



PM appeared in both non infected group and the probiotics treated groups. The



infections. While the infected non probiotics treated

decrease in body weight,low of the performance parameters

and characteristics sings and pm lesions for SalmonellaEnteritidis


proteins and albumin in probiotic treated group when compared with other

groups.Results of ELISA

Conference Of &


nfection On

2

Department of Poultry Diseases, Animal Health Research Institute, Qena Laboratory,

Department of Bacteriology&Mycology, Faculty of Veterinary Medicine, South Valley




ombating enteric pathogens. So this study was



isolate studied

wasisolated from chickens from Qena



e control group, second group (2) fed on a balanced

Bio.com. LTD)

Lactobacillus Acidophilus

cfu, in drinking water for 5

SalmonellaEnteritidis 109


Cfu / ml at 7day old and considered the positive




um biochemical parameters and humoral immunity using



treated groups. The



infections. While the infected non probiotics treated

decrease in body weight,low of the performance parameters

SalmonellaEnteritidis infections


when compared with other



assay revealed significant elevation in humoral immune response in the

Probiotics and infected treated groups respectively when compared with control

groups. The immune status assessment clarified that both phagocytic percentage

and indices significantly i

compared with their untreated control group. The study concluded that the use

of probiotics improve the performance parameters which including weekly feed

consumption, weekly body weight gain, main weekly

and improve the immune response of birds against

infection.

Key words: Broilers Chickens, Probiotic, performance parameters, Serum

Biochemistry, Immune response.



28




and indices significantly increased (P≤0.05) in the probiotic treated group as



consumption, weekly body weight gain, main weekly body weights and FCR

and improve the immune response of birds against SalmonellaEnteritidis

Broilers Chickens, Probiotic, performance parameters, Serum

Biochemistry, Immune response.

Conference Of &





≤0.05) in the probiotic treated group as



body weights and FCR


Broilers Chickens, Probiotic, performance parameters, Serum



Evaluation of ELISA

Diagnosis Riad, E.M., Amany, N. dapgh., and Dalia M. Mohsen

Animal Health Rsearch Institute

Paratuberculosis

Mycobacterium avium

Johne's disease( JD) is a Chronic debilitating disease that affects a wide range

of animal hosts. So, this study was designed to focus on diagnosis of

paratuberculosis by traditional culture metho

A total of 165 Egyptian cattle (more than 5 years old) were collected

from some private farms in different districts at El Sharkia governorate,. All

diseased (155) cattle were in poor condition with marked reduction in milk

production, chronic or intermittent diarrhea and showed no fever. From which,

fecal , milk and serum samples were collected as well as from other 10 contact

apparently normal animals. The Fecal and milk samples were decontaminated

and were inoculated onto Herrold's eg

supplemented with mycobactin j., and the slants were incubated at 37°c and

observed every 2 weeks for 16 weeks.

The suspected growth represented by grayish white rough colonies were

smeared and stained by Z.N stain then micros

EL1SA was carried out on the serum and milk samples using commercial

ELISA kit as well as the use of modified ELISA.

The obtained results revealed that out of 165 collected samples from. 13

fecal samples were positive for det

diarrheic cows and two samples from 10 apparently healthy contact cows with a

percentage of 7.9 % and 20 % respectively . The results of indirect ELISA by

the using the commercial kit revealed that, 18 serum samp

(10.9%) tested cow samples were positive and were harboring specific

antibodies against MAP ( 16 cases out of 155 diseased animals and two out

of 10 apparently healthy contact cows).

Using ELISA with W. antigen detected 19 positive

examined caws and ELISA with S. antigen detected of (21) positive cases .

The diagnostic sensitivity and specificity for JD, by the S.ELISA where greater

and showed highly detection rate of paratuberculosis infection.

KeyWords:Mycobacterium avium supspecies

Paratuberculosis, Johns disease , ELISA . cattle.



29

Evaluation of ELISA And The Conventional Methods

iagnosis Of JOHNE’ S Disease In Cows Riad, E.M., Amany, N. dapgh., and Dalia M. Mohsen

Animal Health Rsearch Institute - Dokki, Giza.

ABSTRACT

Paratuberculosis. in cattle is an infectious disease caused by

Mycobacterium avium supspecies paratuberculosis (MAP) .Paratuberculosis or



paratuberculosis by traditional culture method and ELISA .




ronic or intermittent diarrhea and showed no fever. From which,



and were inoculated onto Herrold's egg yolk medium (HEYM) slants


observed every 2 weeks for 16 weeks.


smeared and stained by Z.N stain then microscopy examined. The indirect


ELISA kit as well as the use of modified ELISA.


fecal samples were positive for detection of MAP.by culture method from (155)



the using the commercial kit revealed that, 18 serum samples out of 165



of 10 apparently healthy contact cows).

Using ELISA with W. antigen detected 19 positive cases out of 165



and showed highly detection rate of paratuberculosis infection.

cobacterium avium supspecies paratuberculosis,

Paratuberculosis, Johns disease , ELISA . cattle.

Conference Of &


ethods In

Riad, E.M., Amany, N. dapgh., and Dalia M. Mohsen

in cattle is an infectious disease caused by

paratuberculosis (MAP) .Paratuberculosis or






ronic or intermittent diarrhea and showed no fever. From which,



g yolk medium (HEYM) slants



copy examined. The indirect



ection of MAP.by culture method from (155)



les out of 165



cases out of 165



paratuberculosis,



A StudyOn Bacterial

1Mohamed E. A. Nasef &

1Food Hygiene Dep., Animal Health Research 2Bacteriology Dep., Animal Health Research Institute, Damietta Branch, ARC, A.R.E.

A total of 120 random samples of milk were collected from dairy cows in

some examined dairy farms located at different centers of Damietta

governorate. California Mastitis Test (CMT), bacteriological and mycological

examination were carried out for detecti

mastitis. The obtained results revealed that the most bacterial isolates were

Staph.aureus, Escherichiacoli

Streptococcusuberis, Corynebcteriumbovis

prevalence rate of 40%, 30%, 10%, 8.3%, 3.3% and 1.7%, respectively. While,

the most frequently mycotic isolate was

11.7%. Also, the obtained results revealed mixed infection of

C.albicans in 4 samples and

samples of cow's milk having subclinical mastitis. None of

which isolated from mastitic milk samples produce enterotoxins A, B, C, D or

E. In Vitro, antimicrobial suceptiblity test of the

the most effective antibiotics were Ciprofloxacin, Levofloxacin, Cefataxime

and Amoxycillin/Clavulanic acid. While, all bacterial isolates except

were resistant to Cephalexin and Streptomycin.Also, all bacterial is

except Strept.uberis were resistant to Erythromycin. The most effective

antimycotic against Candidaalbicans

conclusion, recommendation and preventive measures of subclinical mastitis

were discussed.

Key Words: Staph.aureus,

Streptococcus uberis, Candidia albicans



30

acterial And Fungal Causes Of Subclinical

Mastitis In Dairy Cows

Mohamed E. A. Nasef &2 Rehab E. DawodFood Hygiene Dep., Animal Health Research Institute, Damietta Branch, ARC, A.R.E.

Bacteriology Dep., Animal Health Research Institute, Damietta Branch, ARC, A.R.E.

ABSTRACT




examination were carried out for detection of positive cases of subclinical


Escherichiacoli, coagulase-negative staphylococci,

Corynebcteriumbovis and Pseudomonasaeruginosa

lence rate of 40%, 30%, 10%, 8.3%, 3.3% and 1.7%, respectively. While,

the most frequently mycotic isolate was Candidiaalbicans with an incidence of

11.7%. Also, the obtained results revealed mixed infection of Staph.aureus

in 4 samples and mixed infection of E.coli and C.albicans

samples of cow's milk having subclinical mastitis. None of Staph.aureus


E. In Vitro, antimicrobial suceptiblity test of the bacterial isolates revealed that


and Amoxycillin/Clavulanic acid. While, all bacterial isolates except

were resistant to Cephalexin and Streptomycin.Also, all bacterial is

were resistant to Erythromycin. The most effective

Candidaalbicans was Fluconazole. The obtained results,


aureus, Escherichiacoli, Pseudomonasaeruginosa

Candidia albicans, Mastitic Milk, Dairy Cow .

Conference Of &


ubclinical

Institute, Damietta Branch, ARC, A.R.E.

Bacteriology Dep., Animal Health Research Institute, Damietta Branch, ARC, A.R.E.




on of positive cases of subclinical


negative staphylococci,

Pseudomonasaeruginosa with

lence rate of 40%, 30%, 10%, 8.3%, 3.3% and 1.7%, respectively. While,

with an incidence of

Staph.aureus and

C.albicans in 2

Staph.aureus strains


bacterial isolates revealed that


and Amoxycillin/Clavulanic acid. While, all bacterial isolates except E. coli

were resistant to Cephalexin and Streptomycin.Also, all bacterial isolates

were resistant to Erythromycin. The most effective

was Fluconazole. The obtained results,


Pseudomonasaeruginosa ,

, Mastitic Milk, Dairy Cow .



Some Studies O

Anguilla O

Fish Disease Dept., Animal Health Research Institute (ARC), Dokki, Giza, Egypt.

The present study deal with 240 Anguilla Anguilla off spring collected

from Lake El Broullus in Egypt and it was conducted to clarify the clinical

pictures. Anal divergence from nor

diagnostic tool of Anguillicoliosis. Also, prevalence, abundance and intensity of

Anguillicola crassuswas recorded. Trials for treatment of 300 wild infected A.

Anguilla was with Artemsa vulgarism

the Artemsa vulgarism

respectively and that treated with levamisol HCl group at 35 and 75 g size were

84 and 92 % respectively was 80%.While, the control group at 35 and 75 g size

were 60 and 80 % respectively. The prevalence of

Artemsavulgarism treated group at 35 and 75 g size were 30 and 23%


30%. While, the control group at 35

Anguillicoliosis can be safely treated using

effects. In this study, Anguillicoliosis can be safely treated using

Artemsavulgarism than levamisol HCl.

Keywords: Anguillicoliosis, Anguilla Anguilla, swim bladder,

Artemsavulgarism, levamisol HCl.



31

On Parasitic AnguillicoliasisInWild Anguilla

Offspring In Lake El Broullus, Egypt

Abeer, E. Mahmoud Disease Dept., Animal Health Research Institute (ARC), Dokki, Giza, Egypt.



pictures. Anal divergence from normal to red colouration could be used as a


was recorded. Trials for treatment of 300 wild infected A.

Artemsa vulgarism and levamisol HCl. The survivability of

Artemsa vulgarism treated group at 35 and 75 g size were 92 and 96%



re 60 and 80 % respectively. The prevalence of A. crassus

treated group at 35 and 75 g size were 30 and 23%


30%. While, the control group at 35 and 75 g size were 70%. In this study,

Anguillicoliosis can be safely treated using Artemsavulgarism without any side


than levamisol HCl.

Anguillicoliosis, Anguilla Anguilla, swim bladder,

, levamisol HCl.

Conference Of &


ild Anguilla

n Lake El Broullus, Egypt

Disease Dept., Animal Health Research Institute (ARC), Dokki, Giza, Egypt.



mal to red colouration could be used as a


was recorded. Trials for treatment of 300 wild infected A.

. The survivability of




infection in



and 75 g size were 70%. In this study,

without any side


Anguillicoliosis, Anguilla Anguilla, swim bladder,



The ThirdSession

Name (Prof. Dr.)

Mostafa Shalaby

George Rogers

Gehan Gamil Eman El saeed

Mohammed Abd El Badee

1- Hepatoprotective Effect of Jojoba Oil and Nigella Sativa

Seeds in Rats Fed Diet

2- Some studies on the effects of kemzyme and/or

salinomycin supplementation on growth performance and

certain heamatological parameters in broiler chicks

3- Toxic Effects of Cypermethrin on Male fertility and

some hepatic biochemi

Rats

4- Ameliorating Effect of White Tea Extract against

Acrylamide Induced DNA Damage and Biochemical

Changes in Male Rats

5- Cytogenetic and pathological studies on the effect of

Gibberellic acid in rabbit

6- Validation of Melamine and Cyanuric acid analysis using

LC-MS/MS.

7-Validation of HPLC Method for determination of

Doxycycline in Turky Serum



32

ession Is In TheSmallTraining Hall, AHRI

بالمعھدبالقاعة الصغر� الثالثةالجلسة


Job description

7

Professor Dep. of harmacology,Cairo Univ

Mostafa Shalaby

Professor and Chair, Dep. of Pharmacology and Toxicology Distinguished University Scholar

Pathology dep. , AHRI Head of Toxicol. & Forensic

medicine Cairo univ. Pathology dep. , AHRI Mohammed Abd El

Title

Hepatoprotective Effect of Jojoba Oil and Nigella Sativa

Seeds in Rats Fed Diet Containing Aflatoxin

Some studies on the effects of kemzyme and/or

salinomycin supplementation on growth performance and

certain heamatological parameters in broiler chicks

Toxic Effects of Cypermethrin on Male fertility and

some hepatic biochemical parameters in male albino

Ameliorating Effect of White Tea Extract against

Acrylamide Induced DNA Damage and Biochemical

Changes in Male Rats

Cytogenetic and pathological studies on the effect of

Gibberellic acid in rabbit

Melamine and Cyanuric acid analysis using

Validation of HPLC Method for determination of

Doxycycline in Turky Serum

Conference Of &


all, AHRI


7

Page

33 34 35 36 37 38

39

Toxic Effects of Cypermethrin on Male fertility and

cal parameters in male albino



Hepatoprotective Effect of Jojo

Rats Fed Diet Containing Aflatoxin

*Hanan, M. Sobhy; **Enas ,K. Abo Elmagd and

*Biochem.Toxicol &Feed Deff. Dep. AHRI and ** microbiology Dep.

Aim: This study aimed to evaluate the

Nigella sativa seeds in rats fed diet contaminated with aflatoxin.

Methods: Forty mature male rats were divided into eight groups and treated

for 30 days as follows: (1) Untreated control; (2) Jojoba oil 1.25% (lo

concentration) ; (3) Jojoba oil 2.5% (high concentration) ; (4) Aflatoxin (AF)

contaminated diet (30ug/kg diet); (5) low dose Jojoba oil plus AF; (6) high dose

Jojoba oil plus AF; (7) Nigella sativa seeds at 2.5% and (8) Nigella sativa seeds

plus AF. Blood samples were collected for biochemical analyses and livers

were taken DNA measurement.

Results: The results indicated that Jojoba oil is rich in protein, phenolic

compounds, phytic acid, and considerable amounts of simmondsin. Animals fed

AF -contaminated diet showed severe biochemical changes and DNA damage.

Feeding on ration mixed with Jojoba oil alone at the two tested

concentrations or Nigella sativa seeds did not induce significant alterations in

all tested parameters. Treatment with Jojoba oi

protected against hepatotoxicity and DNA damage induced by AF.

Conclusion: It can be concluded that Jojoba oil and Nigella sativa seeds can be

incorporated in AF-contaminated feed to protect against hepatotoxicity and

DNA damage induced by AF.



33

Hepatoprotective Effect of Jojoba Oil and Nigella Sativa Seeds

Rats Fed Diet Containing Aflatoxin

Hanan, M. Sobhy; *Mohammed ,K. Abo Elmagd,*Maha, M .El

Enas ,K. Abo Elmagd and *Eman .Sh .Laz

*Biochem.Toxicol &Feed Deff. Dep. AHRI and ** microbiology Dep.- Faculty of medicine for

girls – Al-Azhar university

ABSTRACT

This study aimed to evaluate the hepatoprotective effect of Jojoba oil and

Nigella sativa seeds in rats fed diet contaminated with aflatoxin.

Forty mature male rats were divided into eight groups and treated

for 30 days as follows: (1) Untreated control; (2) Jojoba oil 1.25% (lo

concentration) ; (3) Jojoba oil 2.5% (high concentration) ; (4) Aflatoxin (AF)



od samples were collected for biochemical analyses and livers

were taken DNA measurement.

The results indicated that Jojoba oil is rich in protein, phenolic


ed diet showed severe biochemical changes and DNA damage.

Feeding on ration mixed with Jojoba oil alone at the two tested


all tested parameters. Treatment with Jojoba oil to rats fed AF containing diet


It can be concluded that Jojoba oil and Nigella sativa seeds can be

contaminated feed to protect against hepatotoxicity and

damage induced by AF.

Conference Of &


ba Oil and Nigella Sativa Seeds In

Maha, M .El-kholy,

Faculty of medicine for

hepatoprotective effect of Jojoba oil and

Forty mature male rats were divided into eight groups and treated

for 30 days as follows: (1) Untreated control; (2) Jojoba oil 1.25% (low

concentration) ; (3) Jojoba oil 2.5% (high concentration) ; (4) Aflatoxin (AF)-



od samples were collected for biochemical analyses and livers

The results indicated that Jojoba oil is rich in protein, phenolic


ed diet showed severe biochemical changes and DNA damage.

Feeding on ration mixed with Jojoba oil alone at the two tested doses


l to rats fed AF containing diet


It can be concluded that Jojoba oil and Nigella sativa seeds can be

contaminated feed to protect against hepatotoxicity and



Some Studies On

Supplementation

Heamatological

*Amal,A.,Zaki;Afaf,Saleh,Moussa**Hodaallah,Hatem,Ahmed;

*Animal Health Research Institute Dokki**Physiology Department Cairo University

The present study was designed to investigate the effects of enzyme

(kemzyme; recently used multienzymes) and anticoccidial drugs (salinomycin)

as feed additives on productive performance, metabolic and some heamostatic

parameters in broiler chicks. The study was carried out on 200 male one day

old hubbard chicks. Chicks were assigned into 4 groups (50 chicks / group).1

group was fed on a basal ration for 8 successi

group , 2nd group was fed on a basal ration mixed with kemzyme at a dose of

(100 ppm / kg ration). 3

salinomycin powder at a dose of 100mg / kg ration).

basal ration mixed with kemzyme

/ kg ration).At the end of the experiment, the

increase in body weight, feed efficiency and a significant

conservation ration in groups 2 and

Concerning haemostatic parameters, the levels of PT and APTT were

significantly lowered. On the other hand, there were increase in levels of

fibrinogen. The serum biochemical results showed a significant increase in

levels of glucose, liver glycogen and T

comparison to control one at

of TSH in groups supplemented with kenzyme. There was no significant change

in the level of T4 all su

We concluded that

salinomycin induced more pronounced effect than salinomycin

alone.Supplementation of broilers feed with kemzymes could help poultry

producers adapt to the forthcoming European

and increasing consumer demand for safe affordable food and exploit the

growing demand for natural and organic poultry products.



34

n TheEffects Of kemzyme and/or Salinomycin

upplementation On Growth Performance and Certain

eamatological Parameters in Broiler Chicks

Amal,A.,Zaki;Afaf,Saleh,Moussa**Hodaallah,Hatem,Ahmed;

**Kamal ,A.,Attia *Animal Health Research Institute Dokki**Physiology Department Cairo University



productive performance, metabolic and some heamostatic

parameters in broiler chicks. The study was carried out on 200 male one day

old hubbard chicks. Chicks were assigned into 4 groups (50 chicks / group).1

group was fed on a basal ration for 8 successive weeks and kept as control

group was fed on a basal ration mixed with kemzyme at a dose of

(100 ppm / kg ration). 3rd groupwas fed on a basal ration mixed with

salinomycin powder at a dose of 100mg / kg ration). 4th group was fed on a

ration mixed with kemzyme (100mg / kg ration) and salinomycin (100mg

.At the end of the experiment, the results showed a significant

increase in body weight, feed efficiency and a significant decrease in feed

conservation ration in groups 2 and 4 in comparison to group 1 at p≤ 0.05.



fibrinogen. The serum biochemical results showed a significant increase in

levels of glucose, liver glycogen and T3 in all supplemented groups in

comparison to control one at p≤ 0.05. However, there was a reduction in level


in the level of T4 all supplemented groups.

We concluded that .Kenzyme alone and/or in combination with



producers adapt to the forthcoming European on antibiotic growth promoters


growing demand for natural and organic poultry products.

Conference Of &


alinomycin

ertain

hicks

Amal,A.,Zaki;Afaf,Saleh,Moussa**Hodaallah,Hatem,Ahmed;

*Animal Health Research Institute Dokki**Physiology Department Cairo University



productive performance, metabolic and some heamostatic

parameters in broiler chicks. The study was carried out on 200 male one day-

old hubbard chicks. Chicks were assigned into 4 groups (50 chicks / group).1st

ve weeks and kept as control

group was fed on a basal ration mixed with kemzyme at a dose of

groupwas fed on a basal ration mixed with

group was fed on a

salinomycin (100mg

results showed a significant

decrease in feed

≤ 0.05.



fibrinogen. The serum biochemical results showed a significant increase in the

in all supplemented groups in

However, there was a reduction in level


Kenzyme alone and/or in combination with



on antibiotic growth promoters




Toxic Effects of Cypermethrin

Hepatic Biochemical

Sahar M. El-Sheshtawy*; Lobna S. El

*Toxicology , Animal Health Research Institute, Tanta Lab.,** Bacteriology, Animal Health

Research Institute, Tanta Lab&***Food Hygiene , Anim

This study is aimed to evaluate the effect of Cypermethrin on some serum

biochemical parameters , body weight and fertility in male rats. A total of

thirty albino rats weighing (180g

divided into 3 groups each

mg/kg bw. Second group was given Cypermethrin 25 mg/kg bw. and third

group was left as control group. All doses were orally administered twice

weekly for 65 successive days. At the end of the experime

samples were collected from each rat for biochemical analysis. Rats were

humanely euthanized and testes and epididymis were taken for determination

of weight of sexual organs and epididymal sperm characters in male rats. Oral

administration of Cypermethrin twice weekly for 65 days in a dose of 12.5

mg/kg b.w, and 25 mg/kg.b wt . significantly increased

AST , ALT and ALP, plus increased levels of cholesterol and triglyceride. The

effect on the liver is one of the ma

also a significant decrease in the total protein and albumin synthesized by the

liver. Cypermethrin exposure resulted in a significant decrease in weight of

testis and epididymis, epididymal sperm counts, sp

sperm abnormalities

being less with low Cypermethrin dose and more even extensive with high

Cypermethrin dose .

hepatorenal cell function. In addition , cypermethrin has dangerous effect on

male fertility due to its effect on spermatogenisis (sperm number, motility and

sperm abnormalities)

Key word:Cypermethrin ,serum biochemistry , male fertility



35

Toxic Effects of Cypermethrin On MaleFertility and

iochemical Parameters In Male Albino Rats

Sheshtawy*; Lobna S. El- Gebaly** , Maha R. B. Ebaid** and

Nabila I. El-Sheikh***


Research Institute, Tanta Lab&***Food Hygiene , Animal Health Research Institute, Tanta Lab

ABSTRACT



thirty albino rats weighing (180g -200g) were used for this study. Rats were

divided into 3 groups each of 10 rats. First group was given Cypermethrin 12.5



weekly for 65 successive days. At the end of the experimental period, blood




tion of Cypermethrin twice weekly for 65 days in a dose of 12.5

mg/kg b.w, and 25 mg/kg.b wt . significantly increased the leakage enzymes


effect on the liver is one of the main toxic effects of this product as there was


Cypermethrin exposure resulted in a significant decrease in weight of

testis and epididymis, epididymal sperm counts, sperm motility and increase in

In conclusion All these changes were dose dependent,


Cypermethrin dose . In conclusion Cypermethrinhas negative effects on

al cell function. In addition , cypermethrin has dangerous effect on


Cypermethrin ,serum biochemistry , male fertility

Conference Of &


ertility and Some

lbino Rats

Gebaly** , Maha R. B. Ebaid** and


al Health Research Institute, Tanta Lab



200g) were used for this study. Rats were

of 10 rats. First group was given Cypermethrin 12.5



ntal period, blood




tion of Cypermethrin twice weekly for 65 days in a dose of 12.5

the leakage enzymes


in toxic effects of this product as there was


Cypermethrin exposure resulted in a significant decrease in weight of

erm motility and increase in

All these changes were dose dependent,


has negative effects on

al cell function. In addition , cypermethrin has dangerous effect on




Ameliorating Effect o

Induced DNA Damage and Biochemical Changes in Male Rats

*Hanan, M. Sobhy; *

K.Abo Elmagdand

*Biochemistry, Toxicology and Feed Deficiency

Dep.- Faculty of medicine for girls

In this study, the protective effect of white tea (WT) aqueous extract

against cytogenicity, DNA damage and some biochemical changes induced by

acrylamide (ACR) in male albino rats was investigated. Six groups containing 5

rats each were used in this study. Group 1 served as a control. Groups 2 and 3

were given single daily oral doses of 10 and 20 mg /kg. b. wt. for 15 successive

days. Group 4 permit

drinking fluid. Groups 5 and 6 were given the same doses as group 2 and 3 in

combination with solubilized WT. Rats received acrylamide in both tested

concentrations significantly decreased the amount of

significantly increased in the percentage of MPCEs and PCE/NCE ratio as

compared with the control group. Administration of acrylamide to rats

significantly increased serum transaminases (AST and ALT), alkaline

phosphatase (ALP), urea,

and high), while it significantly decreased reduced glutathione. Administration

of WT to intoxicated rats normalized all the tested parameters.

Conclusion: Dietary intake and occupational exposure to acryl

must be restricted besides raising the public awareness to ACR hazards. The

white tea extract exhibits a protective effect against ACR damage.

Keywords: White tea, Acrylamide



36

Ameliorating Effect of White Tea Extract against Acrylamide


*Maha, M. El-Kholy; *Eman, Sh. Laz;* Mohammed

Abo Elmagdand **Enas ,K. Abo Elmagd**

*Biochemistry, Toxicology and Feed Deficiency Department, AHRI, Dokki,.** microbiology

Faculty of medicine for girls – Al-Azhar university

ABSTRACT



ylamide (ACR) in male albino rats was investigated. Six groups containing 5



days. Group 4 permitted free access to solubilized WT (1.5%) as the sole



concentrations significantly decreased the amount of DNA/gm of spleen and




phosphatase (ALP), urea, creatinine and malondialdehyde in both doses (low


of WT to intoxicated rats normalized all the tested parameters.

Dietary intake and occupational exposure to acrylamide (ACR)


white tea extract exhibits a protective effect against ACR damage.

Acrylamide, DNA damage, Cytogenicity

Conference Of &


f White Tea Extract against Acrylamide


Mohammed,

Department, AHRI, Dokki,.** microbiology



ylamide (ACR) in male albino rats was investigated. Six groups containing 5



ted free access to solubilized WT (1.5%) as the sole



DNA/gm of spleen and




creatinine and malondialdehyde in both doses (low


amide (ACR)




Cytogenetic A

M. Abdou I, Mervat A Ayoub* and Maha M El Aalem*Biochemistry and Pathology* Dept., Animal Health Research Institute, Zagazig Lab.

This study was designed to clarify the effect of the plant growth

gibberellic acid (GA3) on the cytogenetic and pathological profile of adult

male rabbits. Thirty bucks were classified into 3 equal groups; a control and

two other treated groups; the first (indirectly exposed group, IEG) was forced

to feed on a previously sprayed green fodder (alfalfa) with the recommended

dose of GA3, while the second (directly exposed group, DEG) was forced to

drink ad libitum on 75 ppm GA3 in water for 30 successive days.

Both exposed groups evoked a significant increase

cells and total chromosomal aberrations of bone marrow cells, however, the

most predominated chromosomal aberrations were deletions, ring

chromosomes, end to end associations and aneuploidy, in addition to DNA

damage using comet tes

significant increase in the percentage of these aberrations not only comparing

to the control but also with the first treated group (IEG). Pathological changes

in the liver, kidneys, lungs and testes were reported

These changes were severe in the second treated group (DEG). After one

month recovery period, the deviated parameters of the first treated group (IEG)

were nearly returned to the normal values, meanwhile, the second treated group

(DEG) still revealed significant changes compared to either the control or the

first treated group (IEG).

From this study, It could be concluded that GA3 has a genotoxic and cytotoxic

effects. These effects were severe in rabbits directly exposed to the ho

(DEG) compared to those fed on sprayed green fodder with its recommended

dose (IEG).



37

AndPathological Studies On The Effect

Gibberellic Acid In Rabbit

M. Abdou I, Mervat A Ayoub* and Maha M El Aalem*Biochemistry and Pathology* Dept., Animal Health Research Institute, Zagazig Lab.

ABSTRACT

This study was designed to clarify the effect of the plant growth




reviously sprayed green fodder (alfalfa) with the recommended


drink ad libitum on 75 ppm GA3 in water for 30 successive days.

Both exposed groups evoked a significant increase in the total aberrated




damage using comet test. The second treated group (DEG) showed a



in the liver, kidneys, lungs and testes were reported in both treated groups.




DEG) still revealed significant changes compared to either the control or the

first treated group (IEG).


effects. These effects were severe in rabbits directly exposed to the ho


Conference Of &


ffect Of

M. Abdou I, Mervat A Ayoub* and Maha M El Aalem* Biochemistry and Pathology* Dept., Animal Health Research Institute, Zagazig Lab.

This study was designed to clarify the effect of the plant growth hormone,




reviously sprayed green fodder (alfalfa) with the recommended


in the total aberrated




t. The second treated group (DEG) showed a



in both treated groups.




DEG) still revealed significant changes compared to either the control or the


effects. These effects were severe in rabbits directly exposed to the hormone




Validation of Melamine and Cyanuric

Hamza A. H.1, Gihan M. El Moghazy

1 Genetic Engineering and Biotechnology Research Institute

2 Regional Centers for Food and Feed, Agriculture Research Center

A liquid chromatography

validated to be used in the analysis of Melamine a

food/feed substrates. Validation results revealed that, the Limit of detection and

Limit of quantitation values were 5ng/ml and 10ng/ml for Melamine, 1ng/ml

and 5ng/ml for Cyanuric acid, respectively. The Accuracy percentage v

ranged from 94.4% to 104% for Melamine and 99.1% to 104% for Cyanuric

acid and the linearity regression values were 0.9998 for Melamine and 1.0 for

Cyanuric acid. It can be concluded that, LC

specific and accurate techn

quantitative analysis of Melamine and Cyanuric acid in different food/feed

categories.

Key words: Melamine, Cyanuric acid, LC

Quantitation, linearity, accuracy, daughter ions,



38

Validation of Melamine and Cyanuric Acid Analysis U

MS/MS

Gihan M. El Moghazy2, Hoda M. Abass1 and

Hamouda2

and Biotechnology Research Institute, University of Sadat city

2 Regional Centers for Food and Feed, Agriculture Research Center

ABSTRACT

A liquid chromatography-tandem mass spectrometry based method was

validated to be used in the analysis of Melamine and Cyanuric acid in different



and 5ng/ml for Cyanuric acid, respectively. The Accuracy percentage v



Cyanuric acid. It can be concluded that, LC-MS/MS technique is a reliable,

specific and accurate technique which can be used for qualitative and


: Melamine, Cyanuric acid, LC-MS/MS, Limit of Detection, Limit of

Quantitation, linearity, accuracy, daughter ions, Food.

Conference Of &


Using LC-

and M. S.

University of Sadat city

tandem mass spectrometry based method was

nd Cyanuric acid in different



and 5ng/ml for Cyanuric acid, respectively. The Accuracy percentage values



MS/MS technique is a reliable,

ique which can be used for qualitative and


MS/MS, Limit of Detection, Limit of



Validation Of HPLC Method

Nahla S. Elshater*, Mai A. Fadel*, Fatma Hussein*, Marwa Ragab*, Shimaa H. Nassar*, M.A.M Saleh*, Maha Sabry** and Heba Hassan*

*Reference Laboratory for Veterinary Quality

** Biochemist,Toxicol.& feed Deff. Dept

An accurate, sensitive and reproducible high performance liquid

chromatographic (HPLC) method for the quantitation of do

serum has been developed and validated. The drug and the internal standard

were eluted from a C18 column (4.6 mm i.d., 250 mm,5 µm particle size) with a

mobile phase consisting of 5% acetic acid : methanol : acetonitrile (55:20:25,

v/v/v). A UV detector set at 347 nm was used to monitor the effluent. Retention

time of doxycycline was approximately at 4 min and the run time was 10 min.

The column temperature adjusted at 10

injection volume 25 µl. Doxycyclin

Linearity was confirmed in the concentration range 0.1

quantification in serum. The analytical conditions gave a recoveries in a range

of 93.1% to 103.88%. The limit of detection (LOD) was 0

of quantification (LOQ) was 0.008 µg/ml. The relative standard deviation (RSD

%) was less than 3% in all cases. The developed method was accurate, precise

and rapid for estimation of doxycycline in turkey serum samples.

Keywords: Validation, Doxycycline, Serum, HPLC



39

f HPLC Method For Determination Of Doxycycline

In Turkey Serum


*Reference Laboratory for Veterinary Quality Control on Poultry Production, &

Biochemist,Toxicol.& feed Deff. Dept Animal Health Research Institute, Dokki

ABSTRACT


chromatographic (HPLC) method for the quantitation of doxycycline in turkey




/v). A UV detector set at 347 nm was used to monitor the effluent. Retention


The column temperature adjusted at 100C with a flow rate 1ml/min and

injection volume 25 µl. Doxycycline was extracted from turkey serum by ACN.

Linearity was confirmed in the concentration range 0.1-1 µg/ml for doxycycline


of 93.1% to 103.88%. The limit of detection (LOD) was 0.002 µg/ml and limit



and rapid for estimation of doxycycline in turkey serum samples.

ation, Doxycycline, Serum, HPLC

Conference Of &


f Doxycycline


Control on Poultry Production, &

Animal Health Research Institute, Dokki


xycycline in turkey




/v). A UV detector set at 347 nm was used to monitor the effluent. Retention


C with a flow rate 1ml/min and

e was extracted from turkey serum by ACN.

1 µg/ml for doxycycline


.002 µg/ml and limit





The Fourth S

(

Name (Prof. Dr.)

Khaled Shawky

Samia ElZeini Ibrahim El seedy Omaima El tahir

1- Effect of Chito

production and performance of laying hens

2- Haemato-biochemical alterations in cattle suffering from

anaemia and their effect on quality of some meat

3- Experimental study on the effect of different

cooking method of oxytetracycline r

meat

4- Nitrate and nitrite in some meat products5- Detection of Some Antibiotics Residues in Chicken Meat

and Chicken Luncheon

6- Incidence of biogenic amines and its relation to chemical

preservatives effects in canned beef and Tuna.

7- Detection of harmful residues in some fish species

8- The use of Enzyme Linked Immun

(ELISA) for preliminary screening of

medroxyprogesterone acetate (MPA) and 17β

hormones in marketed slaughtered chicken.

9- Correlation between

products in dairy animals fed on aflatoxin B1

contaminated ration.



40

Session Is In Hall 2, International Center

ForAgriculture

)2قاعة ( الجلسة الرابعة بالعالقات الخارجیة


Job description

9 Food hygiene dep. AHRI Khaled Shawky

Bioch. dep. AHRI Bioch. dep. AHRI Ibrahim El seedy

Food hygiene dep. AHRI

Title

Effect of Chito-oligosaccharide as feed additives on egg

production and performance of laying hens

biochemical alterations in cattle suffering from

anaemia and their effect on quality of some meat

Experimental study on the effect of different

cooking method of oxytetracycline residues in chicken

Nitrate and nitrite in some meat products Detection of Some Antibiotics Residues in Chicken Meat

and Chicken Luncheon

Incidence of biogenic amines and its relation to chemical

preservatives effects in canned beef and Tuna.

Detection of harmful residues in some fish species

The use of Enzyme Linked Immun-Sorbant Assay


medroxyprogesterone acetate (MPA) and 17β-estradiol

hormones in marketed slaughtered chicken.

Correlation between aflatoxin M1 in milk and milk


contaminated ration.

Conference Of &


enter


9

Page

41 42

43

44

45

46

47

48

49

egg

biochemical alterations in cattle suffering from

Experimental study on the effect of different

esidues in chicken

Detection of Some Antibiotics Residues in Chicken Meat

Incidence of biogenic amines and its relation to chemical

Sorbant Assay


estradiol

aflatoxin M1 in milk and milk




Effect of Chito-

Production

Ghada, I.A.El-Gobary*; Amal, F.M.El

Biochemistry Unit, **Food Hygiene Unit, Animal Health Research Institute, Tanta Laboratory.

This study was carried out to evaluate the effect of Chitozinc and

Chitonal on performance of laying hens. The parameters

production %, egg weight, egg mass and feed conversion ratio as well as lipid

profile in egg yolk. Sixty Lohmann Brown laying hens (32 weeks age) were

assigned to three groups, each of 20 hens. The 1

basal diet only, the 2nd

drinking water and the 3

All groups were kept under observation for 45 days and egg production (EP) %

, Feed intake FI (g/hen),

production (g/hen/day) and feed conversion ratio FCR (Kg feed/Kg egg) were

calculated along the experiment. Eggs were collected from each group three

days before the end of the experiment for biochemical analysis

egg yolk.

The results showed that addition of 1ml of Chitonal /liter to drinking

water improved egg production (EP) % , Feed intake FI (g/hen), average egg

weight (EWT), egg mass production (g/hen/day) and feed conversion ratio FCR

(Kg feed/Kg egg) , and a significant decrease in total lipid, total cholesterol,

low density lipoprotein (LDL) in egg yolk. A significant decrease in very low

density lipoprotein (VLDL), triglyceride, and atherogenic index (AI) and a

significant increase in hi

and 3rd groups was reported. The results clarified that, the use of Chitozincand

Chitonal improved the performance and lipid profile in egg yolk of laying hens.

Keyword:Chito-oligosaccharide, Chitosan,



41

-oligosaccharide As Feed Additives O

roduction And Performance OfLaying Hens

Gobary*; Amal, F.M.El-Zoghby*; Nabila, I.El-

and Aml, S.Hamdy*


ABSTRACT


Chitonal on performance of laying hens. The parameters were: feed intake, egg



assigned to three groups, each of 20 hens. The 1st group (control) was fed on nd group was fed on basal diet +1ml of Chitonal /liter

drinking water and the 3rd group was fed on basal diet + 200 gm. Chitozinc /ton.


, Feed intake FI (g/hen), average egg weight (EWT) in (g) , egg mass



days before the end of the experiment for biochemical analysis of fat content in




feed/Kg egg) , and a significant decrease in total lipid, total cholesterol,



significant increase in high density lipoprotein (HDL) in egg yolk in both 2

groups was reported. The results clarified that, the use of Chitozincand


oligosaccharide, Chitosan, layers, egg, lipids profile.

Conference Of &


On Egg

ens

-Sheikh**



were: feed intake, egg



group (control) was fed on

group was fed on basal diet +1ml of Chitonal /liter

group was fed on basal diet + 200 gm. Chitozinc /ton.


average egg weight (EWT) in (g) , egg mass



of fat content in




feed/Kg egg) , and a significant decrease in total lipid, total cholesterol,



gh density lipoprotein (HDL) in egg yolk in both 2nd

groups was reported. The results clarified that, the use of Chitozincand


layers, egg, lipids profile.



Haemato-biochemical

Anaemia And Hala A. Abd El

Clinicalpathology, and

Nowadays consumers are highly interested in the quality of the products

they eat, especially when this refers to meat

of the anaemia as a factor affect meat quality in ruminants

of different ages, sexes and breeds from different localities, (farms and

abattoirs) in Ismailia Governorate found forty eight animals were anaemic. All

these animals were examined c

situations of anaemia and parasitic infestation. Animals were classified into

three groups, 1st group were healthy cattle free from blood parasite (n=25) used

as control group, 2nd

group were suffering from

collected from each animal for determination of erythrogram and leukogram

changes and some biochemical parameters in separated sera. The results of the

hematological picture revealed a significant reduction in RBC, HB, PCV, MCV

and MCHin cattle infected with

decreases in RBC, HB and PCV with insignificant changes in MCV, MCH and

MCHC (normocytic normochromic anaemia) while

significant increase in MCV and decrease in MCH and MCHC (macrocytic

hypochromic anaemia). Bovine infected with

revealed leukopenia and neutropenia accompanied with

lymphocytosiscompared to control group.The result of biochemically analysis

showed that cattle infected with

reduction in total protein, albumin, globuli

with significant increase in the activities of liver enzymes AST, ALT, total

bilirubin, urea and creatinine. The muscle samples which were taken from

slaughtered anaemic cattle, examined physico

properties, found that pH and (TVBN) values were significantly high whereas

the moisture, protein and fat percent were decreased accompanied with

reduction in (TBA) and iron percent. The muscle from the healthy control

group had significantly higher scor

those from the other anaemic groups.

showing signs of systemic disturbance and elevated temperature should not be

slaughtered but retained for treatment, preferably outside th

Key words: Biochemical, Hematological examination,

babesiosis, Chemical analysis, meat quality, sensory property.



42

biochemical Alterations In Cattle Suffering

nd Their Effect On Quality OfSome MHala A. Abd El-Hamed, Salwa M. Salem andHala N. Ibrahim

Clinicalpathology, and Food hygiene Units, Ismailia, Provincial laboratories,

Animal Health Research Institute, Egypt

ABSTRACT


they eat, especially when this refers to meat so this current analysis is a review

anaemia as a factor affect meat quality in ruminants. Out of (135) cattle



these animals were examined clinically and laboratory to determine their


group were healthy cattle free from blood parasite (n=25) used

group were suffering from Theileriosis (n=28)and 3

group were suffering from Babesiosis(n=20). Two blood samples were



icture revealed a significant reduction in RBC, HB, PCV, MCV

and MCHin cattle infected with theileriosis. Babesiosis induced significant


MCHC (normocytic normochromic anaemia) while theileriosis


hypochromic anaemia). Bovine infected with theileriosis and



showed that cattle infected with theileriosis and babesiosis revealed significant

reduction in total protein, albumin, globulin, glucose and iron levels associated



slaughtered anaemic cattle, examined physico-chemically and sensory

pH and (TVBN) values were significantly high whereas



group had significantly higher scores for tenderness and overall likeness than

those from the other anaemic groups. So it is recommended that the animal


slaughtered but retained for treatment, preferably outside the meat plant.

Biochemical, Hematological examination, Theileriosis

Chemical analysis, meat quality, sensory property.

Conference Of &


uffering From

Meat andHala N. Ibrahim

Units, Ismailia, Provincial laboratories,


current analysis is a review

. Out of (135) cattle



linically and laboratory to determine their


group were healthy cattle free from blood parasite (n=25) used

(n=28)and 3rd

Two blood samples were



icture revealed a significant reduction in RBC, HB, PCV, MCV

induced significant


eileriosis induced


and babesiosis



revealed significant

n, glucose and iron levels associated



chemically and sensory

pH and (TVBN) values were significantly high whereas



es for tenderness and overall likeness than

So it is recommended that the animal


e meat plant.

Theileriosis and



Experimental

Method Of O

Nashwa M. Zaki *, Arwa H. Nassar *, Saleh Shafik* and Nahla S.L.

*Food Hygiene Dept. Animal Health Research Institute

** Reference Lab for Quality Control on Poultry Production Animal Health Research Insti

Tetracyclines (TCs) are licensed for use in a wide range of livestock

species including chicken, and can be administrated either orally or by

injection, resulted in the presence of TCs residues in their edible tissues,

intended for human consumption, causing a health threat.Hence, the stability of

TCs residues in chicken tissues under cooking conditions is an important

research area. The present study was conducted to determine the effect of

differentcooking process like boilin

and liver of 40 broilerchicken which experimentally injected by oxtetracycline

for 3 successive days and analyzed by HPLC. The obtained results showed a

reduction in concentration of oxytetracycline residues afte

process. Microwave process showed the highest reduction effect on TCs

residues followed by boiling and roasting finally process with average mean

values of 1032±405, 2392±1039 and 3297±1357

with reductionpercentage of 75.4%, 42.3%, and 31.4%for microwave, boiling,

and roasting, respectively. While the average mean values for thigh (2142±899,

4585±1699, and 6592±2335

respectively) moreover , Liver samples recorded 8473±4031, for boiling and

6975±2079 ng /g

(p<0.05).in the antibiotic residues after both boiling ,roasting and microwave

processes for oxytetracycline r

effect on reducing OTC residues. Generally, sufficient cooking temperature and

time can have a significant effect on the reduction of TCs residues and provides

an additional margin of safety for consumers



43

Experimental Study On The Effect Of DifferentC

Oxytetracycline Residues In Chicken M


Elshater** *Food Hygiene Dept. Animal Health Research Institute- Mansoura Cairo, Egypt

** Reference Lab for Quality Control on Poultry Production Animal Health Research Insti

Dokki – Giza

ABSTRACT




d for human consumption, causing a health threat.Hence, the stability of



differentcooking process like boiling, roasting and microwave on muscle tissue



reduction in concentration of oxytetracycline residues after different cooking



values of 1032±405, 2392±1039 and 3297±1357ng /g for breast respectively



4585±1699, and 6592±2335 ng /g for microwave, boiling, and roasting,

pectively) moreover , Liver samples recorded 8473±4031, for boiling and

for roasting. The results show significant reduction


processes for oxytetracycline residues. in this study microwaving had a great



an additional margin of safety for consumers.

Conference Of &


Cooking

Meat


** Reference Lab for Quality Control on Poultry Production Animal Health Research Institute ,




d for human consumption, causing a health threat.Hence, the stability of



g, roasting and microwave on muscle tissue



r different cooking



for breast respectively



for microwave, boiling, and roasting,

pectively) moreover , Liver samples recorded 8473±4031, for boiling and

for roasting. The results show significant reduction


esidues. in this study microwaving had a great





Nitrate

Afaf, M.,Ez

*Food Hygiene; ** Biochemistry Dept.Giza provincial lab.,Animal Health Research

The aim of the present study is to determine the residual nitrates and

nitrites in different meat products (hot dog, Luncheon, oriental sausage and

frankfurter) in Giza markets and its relation to public health.

A total of sixty random samples of the f

(15 of each) were collected from different supermarkets in Giza governorate.

The samples were subjected to chemical determination nitrites as content (ppm)

by spectrophotometer with Griess reagent, and nitrates by the same metho

after reduction to nitrites with cadmium power.

The mean level of nitrite (ppm) in the examined meat products were

(227± 40.3) for hot dog, (74± 8.6) for Luncheon, (354± 88.5) for sausage and

(302±72.8) for frankfurter. The level of nitrate in the same

4.7), (21± 5.6), (42± 13.5) and (31± 5.6) respectively. The minimum and

maximum values of nitrite in meat products were 30

Luncheon, 15-945 for sausage and 30

maximum values of nitrate in meat products were 0

Luncheon,0-150 for sausage and 0

Key words: nitrate, nitrite, Luncheon, hot dog, sausage, frankfurter, meat

product.



44

Nitrate AndNitrite In Some Meat Products Afaf, M.,Ez- El dain* and El-Nemr, S. A.**

Food Hygiene; ** Biochemistry Dept.Giza provincial lab.,Animal Health Research

Institute, Dokki, Egypt.

ABSTRACT



frankfurter) in Giza markets and its relation to public health.

A total of sixty random samples of the four mentioned meat products



by spectrophotometer with Griess reagent, and nitrates by the same metho

after reduction to nitrites with cadmium power.



(302±72.8) for frankfurter. The level of nitrate in the same products were (24±


maximum values of nitrite in meat products were 30-480 for hot dog, 0

945 for sausage and 30-750 for frankfurter. The minimum and

of nitrate in meat products were 0-60 for hot dog,0

150 for sausage and 0-210 for frankfurter.

: nitrate, nitrite, Luncheon, hot dog, sausage, frankfurter, meat

Conference Of &


Food Hygiene; ** Biochemistry Dept.Giza provincial lab.,Animal Health Research



our mentioned meat products



by spectrophotometer with Griess reagent, and nitrates by the same method



products were (24±


480 for hot dog, 0-140 for

750 for frankfurter. The minimum and

60 for hot dog,0-60 for

: nitrate, nitrite, Luncheon, hot dog, sausage, frankfurter, meat



Detection Of Some Antibiotics Residues

Amany M. Ahmed and Maisa M. GareibFood hygiene and Pharmacology Research Unit, Ismailia Provincial laboratories, Animal

A total of 40 random fresh chicken breast and chicken luncheon

samples (20 of each) from different retail markets in Ismailia

governorate, Egypt were collected and examined by high performance

liquid chromatography (HPLC) to evaluate the antibiotic

oxytetracycline and ciprofloxacin. The obtained results indicated that

the mean values of oxytetracycline were

in chicken breast and chicken luncheon respectively, while the mean

values of ciprofloxacin were 0.0

respectively. Oxytetracycline Residues were found to be higher than the

maximum residual limits and Ciprofloxacin residue is lower than

MRL.The obtained results confirmed widespread misuses of antibiotics

especially oxytetracycline in farms and lack of application of

recommended withdrawal times. The public health significance as well

as some recommended measures to improve the quality of such food

articles were discussed.

Key words: chicken meat, chicken luncheon, antibiotics residues,

oxytetracycline, ciprofloxacin, HPLC.



45

f Some Antibiotics Residues In Chicken Meat

Chicken Luncheon

Amany M. Ahmed and Maisa M. Gareib Food hygiene and Pharmacology Research Unit, Ismailia Provincial laboratories, Animal

Health Research Institute, Egypt

ABSTRACT




liquid chromatography (HPLC) to evaluate the antibiotics residues of


the mean values of oxytetracycline were 0.5 ± 0.1 and 1.4 ± 0.2 µg / g


values of ciprofloxacin were 0.02 ± 0.01 and 0.03 ± 0.01 µg/g.

Oxytetracycline Residues were found to be higher than the



ycline in farms and lack of application of



articles were discussed.

chicken meat, chicken luncheon, antibiotics residues,

oxytetracycline, ciprofloxacin, HPLC.

Conference Of &


n Chicken Meat And

Food hygiene and Pharmacology Research Unit, Ismailia Provincial laboratories, Animal




s residues of


0.5 ± 0.1 and 1.4 ± 0.2 µg / g


2 ± 0.01 and 0.03 ± 0.01 µg/g.

Oxytetracycline Residues were found to be higher than the



ycline in farms and lack of application of



chicken meat, chicken luncheon, antibiotics residues,



Incidence OfBiogenic

Preservatives

Abd-Elaaty, E. M

Department of Food Control, Animal Health Research Institute. Shebin El koom

Presence of biogenic amines can lead to several problems for susceptible

consumers. A total of forty six random samples of canned beef and canned tuna

(23 samples of each divided as 20 samples in date of production collected as 10

samples after one month o

expired date and 3 samples expired) were collected from different markets at El

Menofea Governorate for detection of biogenic amines and preservatives in

these samples. The obtained results indicated that

putrescine and tyramine in canned beef samples shortly after production were

1.17± 0.31, 9.31± 2.18 and 3.05± 0.92 mg/100g respectively, the averages in

samples shortly before expirey date are 2.62± 0.43, 12.46± 2.72 and 4.71± 1.

mg/100g for histamine, putrescine and tyramine,respectively. In expired canned

beef samples the averages were 3.93±0.53, 16.41±2.75 and 5.47± 1.33 mg/100g

for histamine, putrescine and tyramine, respectively. In canned tuna samples,

the numbers of unaccepted sampled due to increase the level of histamine in

both examined samples shortly after production and samples before expiring is

10 were 50% of examined samples in the two groups in date of production.

While, all examined samples of expired tuna sampl

increased histamine. Miss handling of meat and bad storage conditions for food

minimize the date of expiring, so care must be taken to keep food in the most

suitable conditions to avoid the spoilage or chemical changes in its com

Keywords: Biogenic amines, CannedBeef,Tuna and preservatives.



46

iogenic Amines And Its Relation To C

reservatives Effects In Canned Beef And Tuna.

Elaaty, E. M1 and Shaimaa , M. Nada2 .

Food Control, Animal Health Research Institute. Shebin El koom

ABSTRACT




samples after one month of production, other 10 samples before two months of



these samples. The obtained results indicated that the average of histamine,



samples shortly before expirey date are 2.62± 0.43, 12.46± 2.72 and 4.71± 1.




epted sampled due to increase the level of histamine in



While, all examined samples of expired tuna samples were unaccepted due to



suitable conditions to avoid the spoilage or chemical changes in its com

Biogenic amines, CannedBeef,Tuna and preservatives.

Conference Of &


Chemical

nd Tuna.

Food Control, Animal Health Research Institute. Shebin El koom




f production, other 10 samples before two months of



the average of histamine,



samples shortly before expirey date are 2.62± 0.43, 12.46± 2.72 and 4.71± 1.15




epted sampled due to increase the level of histamine in



es were unaccepted due to



suitable conditions to avoid the spoilage or chemical changes in its components.



Detection Of

Marzouk, N. M.; Hala M. Shoukry; Hala Ali; Naser, G. A. and Asmaa M.

Food Hygiene Department, Animal Health Research

Heavy metals (Cadmium Cd, Lead Pb and Mercury Hg), organochlorine

pesticides and testesterone residue levels were assessed in some fish species

sold in Cairo Governorate. A total of one hundred samples from five different

fish species Oreochromis niloticus

Lates niloticus (Bagrus Bayad) and,

Thunnus albacares (Tuna) were purchased from vendors in Cairo governorate,

Egypt. Samples were analyzed for Cd, Hg and Pb by Flame Atomic Ab

Spectrometer. The organochlorine pesticides were determined with Agilent gas

chromatograph GC. Methyl testesterone hormone levels were determined wit

Rida screen ELISA kit for tissue. Cd concentrations ranged from

0.0686 ppm for all fish species with highest levels in Tilapia. Total mercury

ranged from 0.075 ppm to

Mackerel. Pb concentrations ranged from

levels in Nile catfish. Organochlorine pesti

BCH, delta- BCH, aldrin, p.p’

DDD, heptachlor, heptachlor epoxide, endosulfan, endrin, gama

methoxychlor) were investigated. Five organochlorine (endrin, p,p’

methoxychlor, gamma-

studied fish samples. Endrin and p,p’

residue in the studied tissues of all fish species except Tuna. The highest values

of Methyl testosterone resid

samples.

Key words: Cadmium Cd

methyl testesterone residue



47

fHarmful Residues In Some Fish Species


Sh. Fayed

Food Hygiene Department, Animal Health Research Istitute,Doki, Giza

ABSTRACT

eavy metals (Cadmium Cd, Lead Pb and Mercury Hg), organochlorine



Oreochromis niloticus (Tilapia), Clarias gariepinus (Nile catfish),

(Bagrus Bayad) and, Scomberomorus cavalla

(Tuna) were purchased from vendors in Cairo governorate,

Egypt. Samples were analyzed for Cd, Hg and Pb by Flame Atomic Ab



Rida screen ELISA kit for tissue. Cd concentrations ranged from 0.0466

fish species with highest levels in Tilapia. Total mercury

ppm to 0.151 ppm for all fish species with highest levels in

Mackerel. Pb concentrations ranged from 0.06ppm to 0 .44 ppm with highest

levels in Nile catfish. Organochlorine pesticides, such as (gamma-BCH, Alpha

BCH, aldrin, p.p’-DDE, endosulfan, dieldrin, p,p’-

DDD, heptachlor, heptachlor epoxide, endosulfan, endrin, gama- chlordan and

methoxychlor) were investigated. Five organochlorine (endrin, p,p’

-BCH and delta- BCH) pesticides were detected in all

studied fish samples. Endrin and p,p’-DDD were the most abundant pesticide


of Methyl testosterone residue were 2 ppb and found in (5) 25% of Tilapia fish

Cadmium Cd- Lead Pb- Mercury Hg- organochlorine pesticides

methyl testesterone residue- fish.

Conference Of &


pecies


Istitute,Doki, Giza

eavy metals (Cadmium Cd, Lead Pb and Mercury Hg), organochlorine



(Nile catfish),

Scomberomorus cavalla (Mackerel),

(Tuna) were purchased from vendors in Cairo governorate,

Egypt. Samples were analyzed for Cd, Hg and Pb by Flame Atomic Absorption



0.0466 ppm to

fish species with highest levels in Tilapia. Total mercury

ppm for all fish species with highest levels in

ppm with highest

BCH, Alpha-

-DDT, p,p’-

chlordan and

methoxychlor) were investigated. Five organochlorine (endrin, p,p’-DDD,

BCH) pesticides were detected in all

DDD were the most abundant pesticide


ue were 2 ppb and found in (5) 25% of Tilapia fish

organochlorine pesticides-



The Use Of Enzyme Linked Immun

Preliminary Screening

And 17β-estradiol

Marzouk, N. M.; Hala A. and Asmaa, M. Sh. FayedFood Hygiene Dep., Animal Health Research Institute Dokki

Administration of hormones to broiler

enhancing purposes may lead to deposit of residues in their carcasses. This

could be a potential human health hazard upon exposure to these residues.

Therefore, this study was carried out on ninety randomly collected fresh broiler

samples to detectmedroxyprogesterone acetate (MPA) and 17β

hormonal residues in muscle and fat of broiler carcasses by usingEnzyme

Linked Immun-Sorbant Assay (ELISA). The mean value of 17β

hormonal residue in muscle and fat of broiler

0.182±0.010 ppb respectively. Sixty eight (75%) of muscle samples and thirty

eight (42%) of fat samples were within the physiological limit (<0.05ppb). 17β

estradiol hormonal residue were within the permissible limit (1ppb) i

examined samples of muscle and fat. Medroxyprogesterone acetate residue

(MPA) could not be detected in all examined samples. The public health

significance and recommendation for the use of hormones were discussed.

Key words: Medroxyprogesterone ace

residue–ELISA- chicken.



48

f Enzyme Linked Immun-Sorbant Assay (ELISA) for

creening Of Medroxyprogesterone Acetate (MPA)

estradiol Hormones In Marketed Slaughtered

Marzouk, N. M.; Hala A. and Asmaa, M. Sh. Fayed Food Hygiene Dep., Animal Health Research Institute Dokki-Giza

ABSTRACT

Administration of hormones to broiler chickens for performance




samples to detectmedroxyprogesterone acetate (MPA) and 17β


Sorbant Assay (ELISA). The mean value of 17β

hormonal residue in muscle and fat of broiler carcasses were 0.090±0.005 and


eight (42%) of fat samples were within the physiological limit (<0.05ppb). 17β

estradiol hormonal residue were within the permissible limit (1ppb) i




: Medroxyprogesterone acetate (MPA)-17β-estradiol hormones

chicken.

Conference Of &


Sorbant Assay (ELISA) for

cetate (MPA)

laughtered Chicken.

Giza

chickens for performance-




samples to detectmedroxyprogesterone acetate (MPA) and 17β- estradiol


Sorbant Assay (ELISA). The mean value of 17β-estradiol

carcasses were 0.090±0.005 and


eight (42%) of fat samples were within the physiological limit (<0.05ppb). 17β-

estradiol hormonal residue were within the permissible limit (1ppb) in all




estradiol hormones



Correlation Between

In Dairy Animals

Seham, N. Homouda*; Mervet, E.El Hasanin*;Tulip,A. Abdelghaffa

Animal Health Research Institute, ARC Tanta Branch.Food Hygien Dept.,*

Chemistry Dept., Animal Health Research Institute, ARC, Dokki. ***

Animal Health Dept.,Fac.Vet.Med., Benha University **

Aflatoxins are cancerogenic compounds produced predominantly by

certain strains of the Aspergillus genus.

hydroxylation of aflatoxin B1( AFB1), which is formed when animals ingest

feed contaminated with aflatoxin B1.

products with aflatoxin M1 is a risk for human health. Afloatoxin M1 is

relatively stable during milk pasteurization and storage as well as during the

preparation of various dairy products. In this study, 80 samples represented by

20 samples of animal feed, 20 of raw milk, 20 of cream and 20 of butter

samples were obtained from suppl

concentration of aflatoxin B1 and M1and were determined by high pressure

liquid chromatography (HPLC).

Presence of AFB1 was detected in animalfeed by a ratio of 55% in

concentrations between 7.16

ppb.While mean value of AFM1 in samples of raw milk, cream and butter (20

of each), were 30%, 25% and 25% with a mean value of 0.429 ± 0.031 , 0.272 ±

0.015 and 0.053 ± 0.003 ppb ,respectively, while the acceptability of posit

samples were 70%,75%, and 90%, respectively according to EOS, EC and

Codex Regulation.

According to the results of this study, it is recommended to implement

the determination of aflatoxin M1 in raw milk, in order to verify the real

situation regarding this toxin and thus exercise control and surveillance

measures which ensure the welfare of the consumer population. It is concluded

that widespread occurrence of AFM1 in milk and its products were considered

to be possible hazards for human health especi



49

etweenAflatoxin M1 In Milk And Milk P

nimals Fed On Aflatoxin B1 Contaminated

Seham, N. Homouda*; Mervet, E.El Hasanin*;Tulip,A. Abdelghaffa

and Khaled, E. ELEkhnawy*** Animal Health Research Institute, ARC Tanta Branch.Food Hygien Dept.,

Chemistry Dept., Animal Health Research Institute, ARC, Dokki.

Animal Health Dept.,Fac.Vet.Med., Benha University

ABSTRACT


certain strains of the Aspergillus genus. Aflatoxin M1(AFM1) is the major


feed contaminated with aflatoxin B1. Contamination of milk and dairy


ely stable during milk pasteurization and storage as well as during the



samples were obtained from suppliers of the dairy company under study. The


liquid chromatography (HPLC).


concentrations between 7.16-69.81ppb with a mean value of 32.06 ± 1.47



0.015 and 0.053 ± 0.003 ppb ,respectively, while the acceptability of posit




g this toxin and thus exercise control and surveillance



to be possible hazards for human health especially children.

Conference Of &


Products

ontaminated Ration

Seham, N. Homouda*; Mervet, E.El Hasanin*;Tulip,A. Abdelghaffar**

Animal Health Research Institute, ARC Tanta Branch.Food Hygien Dept.,

Chemistry Dept., Animal Health Research Institute, ARC, Dokki.


Aflatoxin M1(AFM1) is the major


Contamination of milk and dairy


ely stable during milk pasteurization and storage as well as during the



iers of the dairy company under study. The



a mean value of 32.06 ± 1.47



0.015 and 0.053 ± 0.003 ppb ,respectively, while the acceptability of positive




g this toxin and thus exercise control and surveillance





The Fifth Session

3(

Name (Prof. Dr.)

Mohamed El mosalamy

Emad Rizkallah Azza Kamal Gihan Oaf

1- Incidence of

Molluscus .

2- Effect of vacuum packaging technique and refrigeration

on beef.

3- Detection of Testosterone hormone and 17

Methyltestosterone, residues in some fish products at

retailed Cairo markets.

4- Screening theeffic

plant extracts against the infection of

Salmonella enteritidis

quality.

5- Studies on bacterial infection of cow's milk with special

reference to Mycopasma Bovis

and mastitic milk

6-Bacteriological and clincopathological studies on the effect

of Olive Leaves

multocida.

7-Prevalence of Enterococci and Streptococci in raw milk and some dairy products and thequality



50

ession Is In Hall 3, International Center

ForAgriculture

3قاعة (الجلسة الخامسة بالعالقات الخارجیة


Job description

7

Food hygiene dep.Assuit Univ. mosalamy

Buffalo disease dep. AHRI zkallah Bioch. dep. , AHRI

Food hygiene dep, AHRI

Title

Incidence of Vibrio species in some crustaceans and

Effect of vacuum packaging technique and refrigeration

Detection of Testosterone hormone and 17-

Methyltestosterone, residues in some fish products at

retailed Cairo markets.

Screening theefficacy of some antibiotics and medicinal

plant extracts against the infection of Escherichia coli and

Salmonella enteritidis and their effect on poultry meat

Studies on bacterial infection of cow's milk with special

Mycopasma Bovis recovered from marketing

and mastitic milk

Bacteriological and clincopathological studies on the effect

Olive Leaves in chicken infected with Pasteurella

Prevalence of Enterococci and Streptococci in raw milk and some dairy products and the subsequent alteration on

Conference Of &


enter


7

Page

51

52

53

54

56

58

59



Incidence OfVibrio

Abd-Elaaty, E. M; Dalia, A

Department of Food Control,Bacteriology. Animal Health Research Institute. Shebin El

Crustaceans and Molluscus, as Crab and Oyster are filter

organisms that concentrate microorganisms, especially the human enteric

viruses and pathogenic

identify Vibrio sp. in fresh a

that of public health important as

mimicus and Vibrio vulnificus

methods (16SrRNA and mPCR). The prevalence of

50% and 65% in fresh Crab, marine Crab and Oyster samples

respectivelyexamined by traditional biochemical method appeared in examined

fresh Crab samples as only one

in collected marine Crab s

V. parahaemolyticus (10%) and

was V. alginolyticus

parahaemolyticus (20%) and

Overall, results of the present study indicated that the using of 16SrRNA and

m-PCR allowed rapid and accurate identification of the predominant and

pathogenic Vibrio species which failed to be accurately identified by

biochemical method. Furthermore, presence of

mimicus in examined samples considered a life threatening to human health.

Key words:Clams, V. vulnificus, V. cholerae, V.parahaemolyticus, V.

mimicus, PCR.



51

VibrioSpecies In Some Crustaceans And Molluscus

Elaaty, E. M; Dalia, A. Salim. And Asem, M. Atwa


koomBranch, Egypt.

ABSTRACT

Crustaceans and Molluscus, as Crab and Oyster are filter


viruses and pathogenic Vibrio species. This study aimed to detect and accurate

sp. in fresh and marine Crab and Oyster specimens especially

that of public health important as cholerae, Vibrio parahaemolyticus, Vibrio

Vibrio vulnificususing using biochemical methods and PCR

methods (16SrRNA and mPCR). The prevalence of Vibrio species was 5%,



fresh Crab samples as only one vibrio mimicus species as (5%). Vibrio

in collected marine Crab samples was V.alginolyticus (15%), V. cholera

(10%) and V. vulnificus (20%). Vibrio species in oyster

(5%), V. cholera ( 10%), V. mimicus

(20%) and V.vulnificus (25%).

lts of the present study indicated that the using of 16SrRNA and

PCR allowed rapid and accurate identification of the predominant and

species which failed to be accurately identified by

biochemical method. Furthermore, presence of Vibrio cholerae and Vibrio

in examined samples considered a life threatening to human health.

, V. vulnificus, V. cholerae, V.parahaemolyticus, V.

Conference Of &


nd Molluscus

twa


Crustaceans and Molluscus, as Crab and Oyster are filter-feeding


species. This study aimed to detect and accurate

nd marine Crab and Oyster specimens especially

cholerae, Vibrio parahaemolyticus, Vibrio

using using biochemical methods and PCR

species was 5%,



Vibrio species

V. cholera (5%),

species in oyster

V. mimicus (5%), V.

lts of the present study indicated that the using of 16SrRNA and

PCR allowed rapid and accurate identification of the predominant and

species which failed to be accurately identified by

o cholerae and Vibrio

in examined samples considered a life threatening to human health.

, V. vulnificus, V. cholerae, V.parahaemolyticus, V.



Effect OfVacuum

*Azza A.H, Abeer E.A.** and Arwa H. N.****Biochemistry and nutritional deficiency dept.,**Bacteriology dept. and Food Hygiene dept.& ***Animal

The present study was designed to investigate the influence of

packaging on chemical and microbial parameters of beef during frozen and

refrigeration storage. Quality assessment of vacuum packaged beef up to 10

weeks was done by the monitoring of quality, TVB

thiobarbituric acid (TBA)

Enterobacteriaceae count, Psychrotrophic counts, Mesophilic count and lactic

acid bacteria. A total of 100 fresh beef samples, 20 samples of them were

examined biochemically and bacteriologically on zero

experiment and the rest of 80 samples were divided into two main groups, 40

samples each. First main group divided into two subgroups, 20 samples each.

First subgroup (A) stored in freezer without vacuum packaging for ten weeks at

-18°C. The second subgroup (A) were vacuumed by Vacsy system and kept in

freezer for ten weeks at

subgroup, 20 samples each. First subgroup (B) stored on shelf of refrigerator

without vacuum packaging for 5

vacuumed by Vacsy system and stored in cyst of vacuum then all samples of

the second main group were kept on shelf of refrigerator for 5 days at 4°C. 4

samples were taken from each subgroup of the first main gr

weeks until the end of the experiment (ten weeks), also 4 samples were taken

from the second main group (B) daily for 5 days (4 samples from each

subgroup) and subjected to biochemical and bacteriological examination.

Obtained results showed that TVB

of vacuum packaging samples were significantly lower than those in non

vacuum packaging samples. Results indicated that vacuum packaging was

effective in reduce lipid oxidation and increased shelf li

microbial load of vacuum packaging samples was significantly lower as

compared to non-vacuum packaging samples. Results also indicated that

vacuum packaging had a very good effect on the organoleptic properties such

as color, odor and taste during refrigeration, but their was no clear effect during

freezing period on the organoleptic properties. Thus, the employment of

vacuum packaging alone or in combination with other protective strategies is

recommended.



52

acuum Packaging Technique And Refrigeration

Beef.

A.H, Abeer E.A.** and Arwa H. N.*** *Biochemistry and nutritional deficiency dept.,**Bacteriology dept. and Food Hygiene dept.& ***Animal

Health Research Institute- Mansoura Lab.

ABSTRACT

The present study was designed to investigate the influence of



weeks was done by the monitoring of quality, TVB-N, free fatty acids (FFA),

thiobarbituric acid (TBA), Histamine and pH and some bacterial count such as



examined biochemically and bacteriologically on zero day before starting the




18°C. The second subgroup (A) were vacuumed by Vacsy system and kept in

freezer for ten weeks at -18°C. The second main group was divided into two


without vacuum packaging for 5 days at 4°C. The second subgroup (B) were



samples were taken from each subgroup of the first main group (A) every two




showed that TVB-N, (FFA), (TBA), Histamine and pH values

of vacuum packaging samples were significantly lower than those in non


effective in reduce lipid oxidation and increased shelf life of beef. Similarly the


vacuum packaging samples. Results also indicated that


r and taste during refrigeration, but their was no clear effect during



Conference Of &


efrigeration On

*Biochemistry and nutritional deficiency dept.,**Bacteriology dept. and Food Hygiene dept.& ***Animal

The present study was designed to investigate the influence of vacuum



N, free fatty acids (FFA),

, Histamine and pH and some bacterial count such as



day before starting the




18°C. The second subgroup (A) were vacuumed by Vacsy system and kept in

18°C. The second main group was divided into two


days at 4°C. The second subgroup (B) were



oup (A) every two




N, (FFA), (TBA), Histamine and pH values

of vacuum packaging samples were significantly lower than those in non-


fe of beef. Similarly the


vacuum packaging samples. Results also indicated that


r and taste during refrigeration, but their was no clear effect during





Detection

Methyltestosterone

Tahia A. El Said*, Ragaa A.S.R. Faisal* and Sameh M. Samy Abu

Dept. of Chemistry Research*, Food hygiene Department**, Animal Health Research

This study was done to estimate the testosterone and 17

methyl-testosterone residues in 45 samples of fish burger, fish kofta and fish

fingers. Samples were collected randomly from

Cairo markets and were analyzed using Emzyme

Sorbent Assay (ELISA) method. The mean value of testosterone residues in

fish burger, fish kofta and fish fingers were 0.407±0.46µg/kg, 0.105 ± 0.04

µg/kg and 0.615±0.54µg /kg, respectively. While, the mean value of 17

methyltestosterone were 0.81 ± 0.03 µg/kg, 0.94 ± 0.19 µg/kg and 1.01 ±

0.16 µg/kg, respectively. Such values were found to be within the acceptable

daily intake. So it

market fish products does not constitute a public health hazard but we

are need to routinely monitor this chemicals as a food quality control measure.



53

Detection Of Testosterone Hormone And 17

Methyltestosterone, Residues In Some Fish Products A

Cairo markets.

Tahia A. El Said*, Ragaa A.S.R. Faisal* and Sameh M. Samy Abu

Taleb**.


Institute, Dokki, Giza.

ABSTRACT

This study was done to estimate the testosterone and 17

testosterone residues in 45 samples of fish burger, fish kofta and fish

fingers. Samples were collected randomly from different localities at

Cairo markets and were analyzed using Emzyme – Linked Immuno



µg/kg and 0.615±0.54µg /kg, respectively. While, the mean value of 17



daily intake. So it seems that the present status of this hormones in



Conference Of &


-

At Retailed

Tahia A. El Said*, Ragaa A.S.R. Faisal* and Sameh M. Samy Abu-


This study was done to estimate the testosterone and 17-

testosterone residues in 45 samples of fish burger, fish kofta and fish

different localities at

Linked Immuno



µg/kg and 0.615±0.54µg /kg, respectively. While, the mean value of 17-



seems that the present status of this hormones in





Screening TheE

Plant Extracts A

Salmonella enteritidis

Nagla, F. El Shab, Eman, M. E. El Sherbeney, Nermeen F. El Shopary,

Mona F. El Tallawy and Gamal, Animal health research institute, Tanta lab.

The present study was conducted to evaluate the efficacy of some

antibiotics and methanol extracts of both

and Cymbopogon citrates

coli and Salmonella enteritidis

sensitivity test and minimum inhibitory concentration (MIC)and

modelwith regarding to their interactions in broiler chickens

antioxidant activities of pomegranate and its

According to the sensitivity tests levofloxacin and pomegranate peel extract

(PPE) were found to have the most effective antibacterial effect against

enteritidis. The minimum bactericidal concentration (MBC) of PPE was 50

mg/ml and MIC ranged from 1.4 to 23.4 mg/ml. One hundred and twenty, avian

48 broiler one day old chicks, were divided into 8 equal groups: G1:negative

control group without infection, G2: pos

with S. enteritidis (3x 10

treated with levofloxacin (10 mg/kg) and PPE (125 mg/ml), G4: infected group

treated with levofloxacin (10 mg/kg) and PPE (250 mg/ ml), G5: prophylactic

group treated with PPE (500 mg/ ml) before infection, G6: infected grou

treated with levofloxacin (10 mg/kg), G7: infected group treated with PPE (125

mg/ ml) and G8: infected group treated with PPE (250 mg/ ml) via crop gavage.

Blood and meat quality tests were performed after treatment and at the end of

the study at 42 days. Biochemical parameters were included detection of

endogenous antioxidants (Malondialdehyde

and superoxide dismutase (SOD)), total serum protein, albumin, globulin and

albumin/ globulin ratio. Quality tests were included org

sensory tests (PH), total volatile nitrogen (TVN), tissue TBA and

microbiological tests. The data showed that both doses of PPE exert similar

bactericidal activity as the standard antibiotic (levofloxacin) with antioxidant

effect and significantly (P<0.05) improved both blood parameters and meat

quality. Both groups G3 and G5 still have infection. Our biochemical analysis



54

Efficacy Of Some Antibiotics And Medicinal

Against The Infection OfEscherichia coli

Salmonella enteritidis And Their Effect On Poultry

Quality.


Mona F. El Tallawy and Gamal, R. Hasab El Naby.Animal health research institute, Tanta lab.

ABSTRACT


antibiotics and methanol extracts of both Punica granaturm (pomegranate) peel

Cymbopogon citrates (lemon grass) against the infection of

Salmonella enteritidis and compare between them in vitro

sensitivity test and minimum inhibitory concentration (MIC)and

modelwith regarding to their interactions in broiler chickens, evaluating the

antioxidant activities of pomegranate and its effect on poultry meat quality


(PPE) were found to have the most effective antibacterial effect against

. The minimum bactericidal concentration (MBC) of PPE was 50



control group without infection, G2: positive control group orally challenged

(3x 108) CFU/ml at the 10th day of age, G3: infected group



group treated with PPE (500 mg/ ml) before infection, G6: infected grou




s. Biochemical parameters were included detection of

endogenous antioxidants (Malondialdehyde-Thiobarbituric Acid (MDA


albumin/ globulin ratio. Quality tests were included organolyptic examination,




significantly (P<0.05) improved both blood parameters and meat


Conference Of &


edicinal

Escherichia coliAnd

oultry Meat


R. Hasab El Naby.


(pomegranate) peel

(lemon grass) against the infection of Escherichia

in vitro using

sensitivity test and minimum inhibitory concentration (MIC)and in vivo

evaluating the

effect on poultry meat quality.


(PPE) were found to have the most effective antibacterial effect against S.

. The minimum bactericidal concentration (MBC) of PPE was 50



itive control group orally challenged

day of age, G3: infected group



group treated with PPE (500 mg/ ml) before infection, G6: infected group




s. Biochemical parameters were included detection of

Thiobarbituric Acid (MDA-TBA)


anolyptic examination,




significantly (P<0.05) improved both blood parameters and meat




revealed that no significant change was recorded among groups in total serum

protein, albumin, globulin or A/G ratio after treatment or at the end of the

experiment. After treatment G4 showed a remarkable reduction in blood MDA

levels when compared with n

SOD levels after treatment G2 and G5 showed a remarkable decrease while G3,

G4, G6, G7 and G8 showed a significant increase. There was no significant

difference was recorded at the end of the experiment betwee

overall results of this study suggest beneficial use of PPE as natural alternative

therapeutic agent to synthetic antibiotics for treatment of

in broilers’ but not as a prophylactic.PPE

microbes and improving meat quality safely either bacteriologically or

chemically.

Key words:Pomegranate peels extract (PPE), antibacterial activity, minimum

inhibitory concentration, antioxidant,



55




levels when compared with normal or infected control groups. Regarding to



difference was recorded at the end of the experiment between groups. The


therapeutic agent to synthetic antibiotics for treatment of S. enteritidis

in broilers’ but not as a prophylactic.PPE give us a great chance in comba


Pomegranate peels extract (PPE), antibacterial activity, minimum

inhibitory concentration, antioxidant, S. enteritidis, broilers, meat quality.

Conference Of &





ormal or infected control groups. Regarding to



n groups. The


S. enteritidis infection

give us a great chance in combating


Pomegranate peels extract (PPE), antibacterial activity, minimum

, broilers, meat quality.



Studies OnBacterial

Reference To Mycopasma Bovis

Nagati S. F3., Sahar, E.Ouda1Bacteriology department ,

In this study ,Out of 143 cows milk samples which examined

bacteriologically,131 pathogens

microbes was S. aureus

E.coli 23 (12.6٪), C. pyogenes

3 (1.4٪). as well as 27 isolates were identified from 38 examined mastitic

cow`s milk.

The isolated strains of

48٪ (n=13), followed by

lowest persent was Myco. bovis

In subclinical mastitis,

followed by E.coli 48% (n=15),

(n=7) and lowest persent was

examined bulk milk, S. aureus

followed by E.coli 28% , ,

Myco. bovis were not detected.

aureus showed the highest rate of isolation 44

S.agalacteae 25% (n=8) ,

Myco. bovis were not detected.

Two samples of clinically mastitisic cows were identified

Myco.bovis and one sample of subclinical mastitisic cow and all isolated strains

were confirrmed by PCR.

S.aureus isolates showed multidrug resistance ranged from 60%

where 100% of isolates were resistance to tetracycline, amplicillin, cephalothin,

amikacin, clindamycin and lincomycin.

S.agalactea showed multidrug resistance ranged from 60%

where 100% of isolates were resistance to tetracycline, neomycin,

sulfa/trimethoprim and clindamycin.

E.coli showed multidrug resistance ranged from 40%

100% of isolates were resistance to sulfa/trimethoprim and lincomycin.

C.pyogenes showed multidrug resi

where 100% of isolates showed multidrug resistance, and were resistance to



56

acterial Infection Of Cow's Milk With S

Mycopasma BovisRecovered From Marketing

Mastitic Milk

., Sahar, E.Ouda2, Soumaya, S.A. El-Shafii1 and Esraa G

Bacteriology department , 2. Mycolasma Department, Institute /Dokki, 3Animal Health

Research/ Fyoum

ABSTRACT


bacteriologically,131 pathogens. was detected, the most frequently identified

S. aureus 54 (37.8٪) while follwed by S. agalacteae

C. pyogenes 16 (8.4٪), E. faecalis 10 (5.6٪) and

). as well as 27 isolates were identified from 38 examined mastitic

The isolated strains of S. aureus showed the highest rate of isolation

(n=13), followed by S.agalacteae 26٪ (n=7), C. pyogene19٪

Myco. bovis 7٪ (n=2).

In subclinical mastitis, S. aureus showed the highest rate 38

48% (n=15), S.agalacteae 19٪ (n=10), C. pyogene

(n=7) and lowest persent was Myco. bovis 2٪ (n=1). Inas regards

S. aureus showed the highest rate of isolation 37

28% , , C. pyogene 21٪ (n=4) while S.agalacteae

were not detected. In about the examined marketing milk,

showed the highest rate of isolation 44٪ (n=14), followed by

25% (n=8) , E.faecalis 31٪ (n=10) while S.agalacteae,

were not detected.

Two samples of clinically mastitisic cows were identified

and one sample of subclinical mastitisic cow and all isolated strains

were confirrmed by PCR.

isolates showed multidrug resistance ranged from 60%


amikacin, clindamycin and lincomycin.


f isolates were resistance to tetracycline, neomycin,

sulfa/trimethoprim and clindamycin.

showed multidrug resistance ranged from 40%-100%, where




Conference Of &


Special

arketing And

and Esraa G3. Animal Health


was detected, the most frequently identified

S. agalacteae 25 (٪13.2),

) and Myco. bovis

). as well as 27 isolates were identified from 38 examined mastitic

showed the highest rate of isolation

٪ (n=5) and

showed the highest rate 38٪ (n=13),

C. pyogene 13٪

Inas regards to the

showed the highest rate of isolation 37٪ (n=7),

S.agalacteae and

about the examined marketing milk, S.

(n=14), followed by

(n=10) while S.agalacteae, E.coli and

Two samples of clinically mastitisic cows were identified as

and one sample of subclinical mastitisic cow and all isolated strains

isolates showed multidrug resistance ranged from 60%-100%,


showed multidrug resistance ranged from 60%-100%,

f isolates were resistance to tetracycline, neomycin,

100%, where


stance ranged from 61%-100%,




tetracycline, amplicillin, neomycin, sulfa/trimethoprim, amikacin and

gentamicin.

E.faecalis showed multidrug resistance ranged from 20%

100% of isolates were resistance to gentamicin and lincomycin. The public

health concern of different isolated strains was discussed.



57


showed multidrug resistance ranged from 20% -100%, where


health concern of different isolated strains was discussed.

Conference Of &



100%, where




Bacteriological A

Of Olive LeavesIn

Samia I. Afifi

1Department of microbiology Animal Health Research Institute, Beni Suef.

This experiment was conducted to investigate the effects of olive leaf

(OL) in chicken experimentally infected with

the (MIC) of (OL) water extract against

chicks one-month-old were ran

experimental groups (20 birds / each). Chicks of the experimental groups,

group (II) and (III) were intramuscularly injected with 0.2 ml/ birds of 18

hrs. broth culture of P. multocida

(III) given ration containing olive leaves (5gm/kg of ration). Clinical signs,

mortality rates, organ invasion, and somehaemato

were recorded. Blood samples were taken 3,7 and 14 days post infection for

study of RBCs count ,HB concentration , total and differential leucocytic

count , serum AST,ALT, creatinine , urea, triglyceride and cholesterol,

results obtained showed that feeding with OL reduced signs of illness,

decreased mortality rate and invasion of lung, liver

multocida, inhibited the growth of

water, at the same time showed significant increase in RBCs count, HB

concentration and significant reductions in blood levels of AST, ALT urea,

and cholesterol. In conclusion, dietary supplementation with OL reduced

bacterial invasion and improved the haemato

broiler chickens.



58

And Clincopathological Studies On The

n Chicken Infected WithPasteurella multocida

Samia I. Afifi1 and Samia M. Mohamed2

1Department of microbiology – 2 Department of clinical pathology Animal Health Research Institute, Beni Suef.

ABSTRACT


(OL) in chicken experimentally infected with P. multocida and determine

the (MIC) of (OL) water extract against P. multocida. A total of 60 balady

old were randomly assigned to 3 groups, control and two



P. multocida containing 3x108 CFU / ml. Birds of group


mortality rates, organ invasion, and somehaemato-biochemical parameters


count ,HB concentration , total and differential leucocytic



decreased mortality rate and invasion of lung, liver and spleen with

inhibited the growth of P. multocida at (MIC)250 mg/ml distilled



In conclusion, dietary supplementation with OL reduced

bacterial invasion and improved the haemato-biochemical parameter in

Conference Of &


he Effect

multocida.

2 Department of clinical pathology


and determine

. A total of 60 balady

domly assigned to 3 groups, control and two



Birds of group


biochemical parameters


count ,HB concentration , total and differential leucocytic



and spleen with P.

at (MIC)250 mg/ml distilled



In conclusion, dietary supplementation with OL reduced

biochemical parameter in



Prevalence Of Enterococci

Some Dairy Products

Seham, F.Gorgy*. El Asuoty, M.S**. and Saber, A.S** and Abeer,H.Ali***

*Animal Health Research Institute, Damanhour branch, **Alexandria

*Senior Researcher of Bacteriology, **Researcher of Food Hygiene , *** Researcher of

The purpose of this research was to study the prevalence of

and streptococci in milk and some dairy products. Survey included 50 samples

of raw milk, 25 kareish cheese and 25 yoghurt samples which were collected

from El-Behera governorate markets. The results

chemical composition was

total solid, Moisture content, pH values and titratable acidity, respectively. in

raw milk samples; 36.8±0.87

cheese and 36.8±0.87

samples, Respectively. incidence of positive samples for aerobic plate count,

streptococci and enterococci count were 88 , 26 and 22 % in

respectively . 76 , 36 and 36 % in kareish cheese and 56, 28 and 32 % in yoghurt

samples, respectively. with mean values of 1.22x10

and 6.3x103±1.9x103

4.7x103±0.87x103 and 5.7x10

yoghurt samples were 7.6x10

5.5x103±0.64x103, respectively. All isolated

Enterococcus faecalis, S. agalactiae

genus level using specific primer targeting 16s rDNA gene in

Enter. Faecalis;sod A

agalactiae. Desired amplicon for virulence genes were obtained.



59

f Enterococci And Streptococci In Raw M

roducts And The Subsequent Alteration

Quality



branch AHRI, Egypt.

Senior Researcher of Bacteriology, **Researcher of Food Hygiene , *** Researcher of

Bacteriology .

ABSTRACT

The purpose of this research was to study the prevalence of

in milk and some dairy products. Survey included 50 samples


Behera governorate markets. The results revealed that the mean of

chemical composition was 12.95±0.11, 86.34±0.26, 6.3±0.07 and 0.18±0.002


36.8±0.87, 61.76±0.84, 3.87±0.11 and 2.27±0.037

36.8±0.87, 76.48±0.94, 4.33±0.05 and 1.09±0.036


streptococci and enterococci count were 88 , 26 and 22 % in raw milk samples,


samples, respectively. with mean values of 1.22x105±0.1x105, 4.5x10

cfu/ml in raw milk samples; 8.9x10

and 5.7x103±1.6x103 cfu/gm in kareish cheese samples and in

yoghurt samples were 7.6x104±0.59x104, 3.7x103±0.6x10

, respectively. All isolated Enterococcus faecium,

Enterococcus faecalis, S. agalactiae and S. dysgalatiae were confirmed at a

genus level using specific primer targeting 16s rDNA gene in S. dysgalatiae and

sod A gene in enterococcus faecium and cfb

. Desired amplicon for virulence genes were obtained.

Conference Of &


Milk And

lteration On



Senior Researcher of Bacteriology, **Researcher of Food Hygiene , *** Researcher of

The purpose of this research was to study the prevalence of enterococci

in milk and some dairy products. Survey included 50 samples


revealed that the mean of

6.3±0.07 and 0.18±0.002 for


2.27±0.037 in kareish

1.09±0.036 in yoghurt


raw milk samples,


4.5x103±0.7x103

cfu/ml in raw milk samples; 8.9x104±0.76x104,

cfu/gm in kareish cheese samples and in

±0.6x103 and

Enterococcus faecium,

re confirmed at a

. dysgalatiae and

cfb gene in S.



1- Viral Respiratory Diseases of Chicken in Egypt: a Review

2- Antioxidant potential and toxicity of Garlic (

sativum): a Review

3- Trans fat

4- Impact of Water Pollution with Heavy Metals on Fish

Health: (poster)



60

The Sixth Session

الجلسة السادسة

Title

Viral Respiratory Diseases of Chicken in Egypt: a Review

Antioxidant potential and toxicity of Garlic (Allium

): a Review

Impact of Water Pollution with Heavy Metals on Fish

(poster)

Conference Of &


Page

61

62

63

65



Viral Respiratory Diseases

Department of Virology, Institute of Animal Health Research,

Infectious bronchitis, Newcastle disease, infectious laryngotracheitis, avian

influenza, and pneumovirus

respiratory tract of chickens, because of their tendency to change its antigenic

properties Infectious laryngotracheitis has appeared in the broiler industry as a

serious disease. Newcastle is a highly contagious, disease in which all birds in a

flock usually become infected within three to four days. Avian pox is a

relatively slow-spreading viral disease i

nodules on the skin and diphtheritic necrotic membranes lining the mouth and

upper respiratory system. In spite of regular vaccination of chicken flocks in

Egypt with Infectious Bronchitis virus (IBV) vaccine, respirator

lesions had developed.

several countries .In general, the highly pathogenic forms of these diseases are

easily diagnosed mainly because of the high mortality and typical lesions

observed. Pneumo viruses are now widely distributed in the poultry industry,

causing “swollen head syndromes,” which is a term used todescribe the

condition in chickens. Vaccination programs are constantly adjusted to improve

protection against diseases. Improved v

diseases.

Key words: Fowl pox, infectious bronchitis virus, Newcastle disease virus,

infectious laryngotracheitis virus, avian influenza virus, avian pneumovirus).



61

Viral Respiratory Diseases Of Chicken In Egypt( Review

Neven Ramzy Department of Virology, Institute of Animal Health Research,

Dokki, Giza, Ismailia branch.

ABSTRACT


influenza, and pneumovirus are the viruses that more frequently affect the


laryngotracheitis has appeared in the broiler industry as a

Newcastle is a highly contagious, disease in which all birds in a


spreading viral disease in birds, characterized by wart



Egypt with Infectious Bronchitis virus (IBV) vaccine, respiratory and kidney

lesions had developed. Sporadic outbreaks of avian influenza were reported in



Pneumo viruses are now widely distributed in the poultry industry,


Vaccination programs are constantly adjusted to improve

protection against diseases. Improved vaccines are needed to control the

: Fowl pox, infectious bronchitis virus, Newcastle disease virus,


Conference Of &


Review)


are the viruses that more frequently affect the


laryngotracheitis has appeared in the broiler industry as a

Newcastle is a highly contagious, disease in which all birds in a


n birds, characterized by wart-like



y and kidney

Sporadic outbreaks of avian influenza were reported in



Pneumo viruses are now widely distributed in the poultry industry,


Vaccination programs are constantly adjusted to improve

accines are needed to control the

: Fowl pox, infectious bronchitis virus, Newcastle disease virus,




Antioxidant Potential

Sahar M. El-Sheshtawy

*Toxicology, Animal Health Research Institute, Tanta Lab.

Animal Health Research Institute, Kafr El

Throughout history, many different cultures have recognized the potential

use of garlic for prevention and treatment of different diseases. Recent studies

support the effects of garlic and its extracts in a wide scale of applications.

Antioxidant activity of Garlic (Allium sativum) destroy free radicals particles

that can damage cell membranes and DNA. Various preparations of garlic,

specially aged garlic extract (AGE), have been shown to have promising

antioxidant potential. However, the presence

with apparently opposite biological effects, has added to the complexity of the

subject. Raw garlic homogenate has been reported to exert antioxidant potential

but higher doses have been shown to be toxic to the heart, liv

aim of this review was to clear the difference between Garlic preparations with

special references to their bioactive constituents, antioxidant potential and

toxicity of Garlic .

Key word: Garlic, antioxidant, toxicity



62

otential And Toxicity Of Garlic(Allium

( Review)

Sheshtawy*; El-Keredy M.S. Abeer** and Mona F.

Eltalawy***

*Toxicology, Animal Health Research Institute, Tanta Lab.,**Nutritional deficiency diseases.

Animal Health Research Institute, Kafr El- Sheikh Lab.&***Food Hygiene, Animal Health

Research Institute, Tanta Lab.

ABSTRACT




activity of Garlic (Allium sativum) destroy free radicals particles



antioxidant potential. However, the presence of more than compound in garlic,



but higher doses have been shown to be toxic to the heart, liver and kidney. The



Garlic, antioxidant, toxicity

Conference Of &


Allium sativum)

Keredy M.S. Abeer** and Mona F.

**Nutritional deficiency diseases.

Animal Health




activity of Garlic (Allium sativum) destroy free radicals particles



of more than compound in garlic,



er and kidney. The





Biochemistry, nutritional defiancy diseases and toxicology department.

Animal Health Research Institute. Doqqi. Egypt.

The primary dietary source for

“partially hydrogenated oils." In November 2013, the U.S. Food and

Drug Administration (FDA) made a preliminary determination that

partially hydrogenated oils and it considered as no longer generally

Recognized as Safe (GRAS) in human food. Before 1990, very little

attention was known about how

1990, research began identifying the adverse health effects of

Based on these findings, FDA instituted labeling regulations for

fat and consumption has decreased in the US in recent decades,

however some individuals may consume high levels

on their food choices.

Trans-fats are easy to use, inexpensive to produce and last a

long time action. Trans

Many restaurants and fast

because oils with trans

fryers. Several countries (Denmark, Switzerland, and Canada) and

jurisdictions (California, New York City, Baltimore, and Montgomery

County, MD) have reduced or restricted the use of

service establishments.

Trans- fats raise your bad low density lipoprotein (LDL)

cholesterol levels and lower your

cholesterol levels. Eating

heart disease and stroke

developing type two-diabetes

Trans- fats can be found in many foods, including fried foods

like doughnuts, and baked goods including cakes, pie crusts, biscuits,

frozen pizza, cookies, crackers, and stick margarines and other spreads.

Look for trans fat on the ingredient list on food packages. You

determine the amount of

looking at the Nutrition Facts panel

“0 grams of trans fats” if they contain 0 grams to less than 0.5 grams

of trans- fat per serving. You can also spot

ingredient lists and looking for the ingredients referred to as “partially



63

Trans fat (Review)

Basem G.A Fahmy


Animal Health Research Institute. Doqqi. Egypt.

ABSTRACT

The primary dietary source for trans-fats is processed food from


tion (FDA) made a preliminary determination that



attention was known about how trans- fat can harm your health. In the

90, research began identifying the adverse health effects of

Based on these findings, FDA instituted labeling regulations for


however some individuals may consume high levels of trans

fats are easy to use, inexpensive to produce and last a

Trans-fats give foods a desirable taste and texture.

Many restaurants and fast-food outlets use trans-fats to deep

trans-fats can be used many times in commercial



County, MD) have reduced or restricted the use of trans- fats in

service establishments.

fats raise your bad low density lipoprotein (LDL)

cholesterol levels and lower your good high density lipoprotein (HDL)

. Eating trans-fats increases your risk of developing

isease and stroke. It’s also associated with a higher

diabetes.

an be found in many foods, including fried foods



Look for trans fat on the ingredient list on food packages. You

determine the amount of trans- fats in a particular packaged food by

Nutrition Facts panel. However, products can be listed as

fats” if they contain 0 grams to less than 0.5 grams

fat per serving. You can also spot trans- fats by

and looking for the ingredients referred to as “partially

Conference Of &



processed food from


tion (FDA) made a preliminary determination that



fat can harm your health. In the

90, research began identifying the adverse health effects of trans-fats.

Based on these findings, FDA instituted labeling regulations for trans-


trans-fats based

fats are easy to use, inexpensive to produce and last a

fats give foods a desirable taste and texture.

fats to deep-fry foods

fats can be used many times in commercial



fats in food

fats raise your bad low density lipoprotein (LDL) -

od high density lipoprotein (HDL) -

risk of developing

. It’s also associated with a higher risk of

an be found in many foods, including fried foods



Look for trans fat on the ingredient list on food packages. You can

fats in a particular packaged food by

ever, products can be listed as

fats” if they contain 0 grams to less than 0.5 grams

fats by reading

and looking for the ingredients referred to as “partially

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Processed-Foods-Where-is-all-that-salt-coming-from_UCM_426950_Article.jsp

http://www.heart.org/HEARTORG/Conditions/Cholesterol/AboutCholesterol/Good-vs-Bad-Cholesterol_UCM_305561_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/What-Are-My-Risks-For-Getting-Heart-Disease-Infographic_UCM_443749_SubHomePage.jsp

http://www.heart.org/HEARTORG/Conditions/Diabetes/UnderstandYourRiskforDiabetes/Understand-Your-Risk-for-Diabetes_UCM_002034_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Understanding-Food-Nutrition-Labels_UCM_300132_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Understanding-Ingredients-on-Food-Labels_UCM_433234_Article.jsp



hydrogenated oils. Small amounts of

meat and dairy products, including beef, lamb an

The American Heart Association recommends cutting back on

foods containing partially hydrogenated vegetable oils to reduce

fat in your diet and preparing lean meats and poultry without

added saturated and trans

you buy at the store and, when eating out, ask what kind of oil foods are

cooked in. Replace the

unsaturated or poly-unsaturated

recommends that adults who would benefit from lowering LDL

cholesterol reduce their intake of

of saturated fat to 5 to 6% of total calories.

Here are some ways to achieve that: 1. Eat a dietary pattern that

emphasizes fruits, vegetables, whole grains, low

poultry, fish and nuts. Also limit red m

beverages. 2. Naturally oils are occurring in un

oils like canola, safflower, sunflower and olive oil, must used. 3. Look

for processed foods made with unhydrogenated oil rather than partially

hydrogenated or hydrogenated vegetable oils or saturated fat. 4. Use

soft margarine as a substitute for butter, and choose soft margarines

(liquid or tub varieties) over harder stick forms. Look for “0 g trans

on the Nutrition Facts label and no hydrogenated oils in

list. 5. Doughnuts, cookies, crackers, muffins, pies and cakes are

examples of foods that may contain trans

eat them. 6. Limit commercially fried foods and baked goods made with

shortening or partially hydroge

foods very high in fat, but that fat is also likely to be trans

Key words: trans fat, phospholipids, triglyceride, cholesterol.



64

hydrogenated oils. Small amounts of trans- fats occur naturally in some

meat and dairy products, including beef, lamb and butter fat.


foods containing partially hydrogenated vegetable oils to reduce


and trans-fat. Read the Nutrition Facts panel on foods


cooked in. Replace the trans-fats in your diet with

unsaturated fats. The American Heart Association


cholesterol reduce their intake of trans-fat and limit their consumption

of saturated fat to 5 to 6% of total calories.


emphasizes fruits, vegetables, whole grains, low-fat dairy products,

poultry, fish and nuts. Also limit red meat and sugary foods and

beverages. 2. Naturally oils are occurring in un-hydrogenated vegetable



hydrogenated vegetable oils or saturated fat. 4. Use


(liquid or tub varieties) over harder stick forms. Look for “0 g trans

on the Nutrition Facts label and no hydrogenated oils in the ingredients


examples of foods that may contain trans-fat. Limit how frequently you


shortening or partially hydrogenated vegetable oils. Not only are these

foods very high in fat, but that fat is also likely to be trans-fat.

trans fat, phospholipids, triglyceride, cholesterol.

Conference Of &


fats occur naturally in some


foods containing partially hydrogenated vegetable oils to reduce trans-


fat. Read the Nutrition Facts panel on foods


diet with mono-

fats. The American Heart Association


fat and limit their consumption


fat dairy products,

eat and sugary foods and

hydrogenated vegetable



hydrogenated vegetable oils or saturated fat. 4. Use


(liquid or tub varieties) over harder stick forms. Look for “0 g trans-fat”

the ingredients


fat. Limit how frequently you


nated vegetable oils. Not only are these

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Saturated-Fats_UCM_301110_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Monounsaturated-Fats_UCM_301460_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Monounsaturated-Fats_UCM_301460_Article.jsp

http://www.heart.org/HEARTORG/HealthyLiving/HealthyEating/Nutrition/Polyunsaturated-Fats_UCM_301461_Article.jsp



Impact Of Water Pollution

Samaa T. Mohammed

zamzam H. Abdel wahed

a Animal Health Research Institute, Ismailia, Egypt. b Department of Animal and Poultry Hygiene, Faculty of Veterinary Medicine, Suez Canal

University, Ismailia, Egypt.

Introduction: Egypt is the largest aquaculture producer in Africa and the 8

largest globally (FAO, 2013),

986,820 tonnes in 2011. Water is the home of the fish and its quality is one of

the most over looked aspect of pond management until it affects fish production

(Ehiagbonare and Ogundiran,2010).

to sediments and accumulate in aquatic biota causing anemia, disturbance of

physiological functions and mortalities of fish

Material and Method:

juvenile Nile tilapia (Orechromis nilotic

different heavy metals combination (based on previous survey on 3 fish farms)

on:

1-Fish performance (daily Body Weight gain, Feed conversion ratio),

2-Some hematological parameters (Total RBcs

Total and differential leucocytic count

3-Immune parameters with fish challenged with Aermouns sobria intra

peritoneal with 0.1 ml bacterial suspensions (1X107 CFU).

One hundred and fifty six experimental fish were allocated into 4 groups (with

triplicate aquarium for each group) as following:

1-G1 served as control,

2-The other groups treated with different heavy metal concentrations in water

as following:

I-G2 (3.238 mg/L pb, 0.

II-G3 (0.092mg/L pb, 0.73 mg/L cu and 0.053 mg/L cd) and

III-G4(1.917 mg/L pb,0.686 mg/L Cu and 0.022 mg/L cd).

Conclusion: Heavy metal pollution have many deleterious effects on

aquaculture, where it reduce the fish growth rate , caused marked anemia,

leucopenia, lymphopenia, hence , fish are more susceptible to diseases by

lowering the immune statues as inhibiting the antibo

on the phagocytosis mechanism, that represented by increasing the bacterial

colonization in heavy metal grouped fish in comparison with control. also it

cause several abnormalities on fish appearance, as exophthalmia , black



65

f Water Pollution With Heavy Metals On Fish Health

(poster)

Samaa T. Mohammeda

, Marwa A. Hassanb

, Mona M. Abdel wahab

zamzam H. Abdel wahedb

and A. M. Hassanb

Animal Health Research Institute, Ismailia, Egypt.

Department of Animal and Poultry Hygiene, Faculty of Veterinary Medicine, Suez Canal

University, Ismailia, Egypt.

Egypt is the largest aquaculture producer in Africa and the 8

(FAO, 2013), whereas the aquaculture production was about



(Ehiagbonare and Ogundiran,2010). heavy metals change water quality, bind

ments and accumulate in aquatic biota causing anemia, disturbance of

physiological functions and mortalities of fish (Eichler et al., 2006).

Material and Method: The present study was conducted on apparently health

juvenile Nile tilapia (Orechromis niloticus ) for 45 days to evaluate the effect of


Fish performance (daily Body Weight gain, Feed conversion ratio),

Some hematological parameters (Total RBcs and Hb content , Ht% value,

Total and differential leucocytic count

Immune parameters with fish challenged with Aermouns sobria intra

peritoneal with 0.1 ml bacterial suspensions (1X107 CFU).

six experimental fish were allocated into 4 groups (with

triplicate aquarium for each group) as following:

G1 served as control,

The other groups treated with different heavy metal concentrations in water

G2 (3.238 mg/L pb, 0.686 mg/L cu and 0.0222 mg/L cd),

G3 (0.092mg/L pb, 0.73 mg/L cu and 0.053 mg/L cd) and

G4(1.917 mg/L pb,0.686 mg/L Cu and 0.022 mg/L cd).

Heavy metal pollution have many deleterious effects on



lowering the immune statues as inhibiting the antibodies formations, also affect



cause several abnormalities on fish appearance, as exophthalmia , black

Conference Of &


n Fish Health

, Mona M. Abdel wahaba

Department of Animal and Poultry Hygiene, Faculty of Veterinary Medicine, Suez Canal

Egypt is the largest aquaculture producer in Africa and the 8th

on was about



heavy metals change water quality, bind

ments and accumulate in aquatic biota causing anemia, disturbance of

, 2006).

The present study was conducted on apparently health

us ) for 45 days to evaluate the effect of


Fish performance (daily Body Weight gain, Feed conversion ratio),

and Hb content , Ht% value,

Immune parameters with fish challenged with Aermouns sobria intra

six experimental fish were allocated into 4 groups (with

The other groups treated with different heavy metal concentrations in water

Heavy metal pollution have many deleterious effects on



dies formations, also affect



cause several abnormalities on fish appearance, as exophthalmia , black tail ,



pale fish. Heavy metals pollution lead to sever economic losses in addition to

their bio-accumulation in fish muscles which cause sever harm to the consumer



66


accumulation in fish muscles which cause sever harm to the consumer

Conference Of &



accumulation in fish muscles which cause sever harm to the consumer.

third international conference of chemistryand...

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