things to do today observe unknown gm (-) results start urinalysis start gm (+) id –check for...
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Things to Do Today• Observe Unknown gm (-) results• Start Urinalysis• Start gm (+) ID
– Check for hemolysis
– Catalase test
– Coagulase test
– Direct stain colonies
• Observe Throat cultures– Check for hemolysis
– Catalase test
– Gm stain “isolated colonies”
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Oxidase Test
Negative Positive
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Urease Test
Negative Positive
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Methyl Red Test
Negative Positive
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KIA Slants
uninoculated
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KIA Slants
uninoculated
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Citrate test
Negative positive
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The Enterotube Multitest System
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The API System: Various species of Enteobacteriaceae are shown here with differences in reactions that enable them to be identified
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Things to Do Today• Observe Unknown gm (-) results
– Perform oxidase test with culture from KIA slant
– Observe Urease tubes– Perform MR test by adding 6-8 drops MR
reagent to the culture– Observe KIA slants for the various reactions
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Three species of Candida can be differentiated in mixed culture when grown on CHROMagar Candida plates
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Identification of urinary tract pathogens with
differential media (CHROMagar)
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EMB with E. coli
Used with permission
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EMB
Pseudomonas auginosa
Enterobacter aerogenes
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Fig. 15-22
Relative Frequencies of Nosocomial Infections I
John Wiley
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Fig. 15-19
Relative Frequencies of Nosocomial Infections II
John Wiley
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Things to Do Today• Start Urinalysis
– Do a 4 quadrant streak with the urine sample using a metal loop onto the EMB plate
– Perform the “$” streak with the urine sample using the plastic loop onto the BAP plate
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A tale of two Staphs
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BAP Hemolytic Reactions
Used with permission
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Throat Swab
Used with permission
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Catalase test
Positive negative
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Coagulase test• Place a drop of the red latix suspension on
the card.
• Mix a tiny portion of the colony into the mix.
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Things to Do Today
• Start gm (+) ID1. Direct stain with Crystal Violet
2. Check for hemolysis
3. Catalase test
4. Coagulase test
• Observe Throat cultures (Do in this order)1. Check for hemolysis
2. Catalase test and find cat (-) colony
3. Gm stain “isolated colonies” on slide with throat swab
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BAP Hemolytic Reactions
Alpha hemolysis
Beta hemolysisgamma
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Types of hemolysis:
A: Alpha or partial hemolysis of red blood cells results in a greenish zone
B: B-hemolysins, which produce complete breakdown of hemoglobin, causing clear zones to form around colonies on blood agar
A B