Upload File

the tryptophan synthase multienzyme complex: exploring structure-function relationships with x-ray...

  • Home
  • Documents
  • The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis
6
© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

Upload: edith-wilson

Post on 28-Jul-2016

214 views

Category:

Documents


2 download

Report

TRANSCRIPT

Page 1: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

Page 2: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

anu
IMAGE UNAVAILABLE FOR COPYRIGHT REASONS
Page 3: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

Page 4: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

anu
IMAGE UNAVAILABLE FOR COPYRIGHT REASONS
Page 5: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology

Page 6: The Tryptophan Synthase Multienzyme Complex: Exploring Structure-Function Relationships with X-Ray Crystallography and Mutagenesis

© 1990 Nature Publishing Group http://www.nature.com/naturebiotechnology


Mutagenesis Techniques (PDF)

Study and engineering of gene function: mutagenesis I. Why mutagenize? II. Random mutagenesis, mutant selection schemes III. Site-directed mutagenesis,

Prokaryotic Gene RegulationTrp Operon •Tryptophan Absent •Repressor Inactive •Operon ON. Trp Operon •Tryptophan Present •Repressor Active •Tryptophan = Corepressor •Operon

Round table discussion: Genetic Screens and Mutagenesis … · 2013. 8. 8. · Round table discussion: Genetic Screens and Mutagenesis Methods. 1) Mutagenesis • Chemical • Radiation

TRYPTOPHAN Disorders Final

SoxAX Binding Protein, a Novel Component of the Thiosulfate-Oxidizing Multienzyme System in

TRYPTOPHAN METABOLISM Lecture 5... · 2020. 1. 27. · Tryptophan metabolism Tryptophan ron deficiency leads to block this reaction Vit Liver enzyme Tryptophan dioxygenase Other tissue

Transposons Mediated Mutagenesis

Nondegenerate Saturation Mutagenesis · 2020. 3. 9. · SATURATION MUTAGENESIS METHODS 3.1 Core techniques in saturation mutagenesis Whether saturation mutagenesis is degenerate,

Tryptophan and madness

Tryptophan - MITwurtmanlab.mit.edu/static/pdf/928.pdf · tryptophan, especially when combined with an insulin-secreting carbohydrate. (Large tryptophan doses lower brain levels of

INFLUENCED BY DIETARY TRYPTOPHAN …

Swaati tryrosine & tryptophan

Oxidative Modification of Tryptophan-Containing Peptides · 10 tryptophan-containing peptides. Exposing a tryptophan-11 containing peptide to 2,3-dichloro-5,6-dicyano-1,4-benzoqui-12

Tryptophan Scans (280nm)biochemistry

Cell-freeSynthesisandFunctionalCharacterizationof ... · Site-specific Mutagenesis—Mutagenesis was performed using the PCR-based QuikChange Multi Site-directed Mutagenesis kit (Stratagene)

Site-Directed Mutagenesis Gene Staphylococcus ofHistidines ...Site-directed mutagenesis and transformations. By using oligonucleotide-directed mutagenesis, the codon for each of the

Tryptophan-independent pathways of IAA biosynthesis: Orange pericarp (orp) mutant of maize inactive tryptophan synthase a true tryptophan auxotroph IAA

Recombinant engineering of gene function: mutagenesis I. Why mutagenize? II. Random mutagenesis, mutant selection schemes III. Site-directed mutagenesis,

transposon mediated mutagenesis

Tryptophan Side Effects

Tryptophan catabolism reflects disease activity in human ... · increases in tryptophan catabolism. Together, these data indicate IDO-1–mediated tryptophan catabolism is highly

THE OPTIMAL TRYPTOPHAN - LuckyVitamin · containing pure Tryptophan alone, Life Extension® also offers L-Tryptophan. Each capsule provides 500 milligrams of L-Tryptophan. L-Tryptophan

Brain tryptophan concentrations and serotonin synthesis ... · raise brain tryptophan and serotonin 2 h later. Brain tryptophan concentrations and serotonin synthesis are thus responsive

Mutagenesis Poster

TR-071 Bioassay of L-Tryptophan for Possible ... · L-TRYPTOPHAN FOR POSSIBLE CARCINOGENICITY CAS No. 73-22-3 ... L-TRYPTOPHAN FOR POSSIBLE CARCINOGENICITY. ... 3-hydroxyanthranili

Tryptophan Emission Data

Gene Rearrangements in the Evolution ofthe Tryptophan ... · shared by all bacterial tryptophan synthases studied. Oneunusual feature ofthe tryptophan path-wayis that it containsbothphophhorylated

Mouse mutagenesis

Membrane-enclosed multienzyme (MEME) synthesis of 2,7 ... › portal › services › download... · Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives

Site Directed Mutagenesis

Insertional Mutagenesis Strategies

Genomes and mutagenesis

Multiplex Conditional Mutagenesis Using … Conditional Mutagenesis Using Transgenic Expression of ... Heteroduplex mobility assay ... Multiplex Conditional Mutagenesis Using Transgenic

Supporting Information mimic a multienzyme-cascade ... · mimic a multienzyme-cascade antioxidative system Ruizhen Tian, XiaoTong Fan, Shengda liu, Feihu yang, Quan luo, Chunxi Hou,