the time has come to analyze dna profile databases forensic bioinformatics () dan e. krane, ph.d.,...
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![Page 1: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/1.jpg)
The Time Has Come to Analyze DNA Profile
Databases
Forensic Bioinformatics (www.bioforensics.com)
Dan E. Krane, Ph.D., Wright State University, Dayton, OH
![Page 2: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/2.jpg)
National DNA Index System (NDIS)
• Established in 1994, controlled by the FBI.
• Explicit expectation that records would be subject to research and quality control.
• No published research derived from NDIS to date.
![Page 3: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/3.jpg)
Benefits of analyses of anonymized records
• Quality assurance/quality control–Potential for improved search strategies
–Potential for refinements of test kits
• Evaluation of population genetic assumptions used for statistical weights
• Use of real-world data in place of simulations
![Page 4: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/4.jpg)
Review of Victoria State Database
Krane/Paoletti analysis: >11,000 profiles each compared to all others across 9 loci:
Shared alleles Observed occurrences 14 40115 2716 117 1618 0
Aussie Bump
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# Matching
Alleles
14 15 16 17
# Observed 401 27 1 16
300
100
20
1
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![Page 7: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/7.jpg)
Benefits of analyses of anonymized records
• Quality assurance/quality control–Potential for improved search strategies
–Potential for refinements of test kits
• Evaluation of population genetic assumptions used for statistical weights
• Use of real-world data in place of simulations
![Page 8: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/8.jpg)
Benefits of analyses of anonymized records
• Quality assurance/quality control• Evaluation of population genetic assumptions used for statistical weights–Are populations uniform at city/state/region/nation levels?
–Are similar close relatives common?
• Use of real-world data in place of simulations
![Page 9: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/9.jpg)
Popular vote in 2012 by Congressional district. Romney won red districts, Obama won blue districts.
How would you determine the frequency of Obama supporters in Seattle?
Obama
WA 61.2%
?
![Page 10: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/10.jpg)
Popular vote in 2012 by Congressional district. Romney won red districts, Obama won blue districts.
How would you determine the frequency of Obama supporters in Seattle?
Obama
WA 61.2%
Region 60.6%?
![Page 11: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/11.jpg)
Popular vote in 2012 by Congressional district. Romney won red districts, Obama won blue districts.
How would you determine the frequency of Obama supporters in Seattle?
Obama
WA 61.2%
Region 60.6%
U.S. 52.9%
?
![Page 12: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/12.jpg)
Popular vote in 2012 by Congressional district. Romney won red districts, Obama won blue districts.
How would you determine the frequency of Obama supporters in Seattle?
Obama
WA 61.2%
Region 60.6%
U.S. 52.9%
Utah? 24.7%
??
![Page 13: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/13.jpg)
Benefits of analyses of anonymized records
• Quality assurance/quality control• Evaluation of population genetic assumptions used for statistical weights–Are populations uniform at city/state/region/nation levels?
–Are similar close relatives common?
• Use of real-world data in place of simulations
![Page 14: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/14.jpg)
Benefits of analyses of anonymized records
• Quality assurance/quality control• Evaluation of population genetic assumptions used for statistical weights
• Use of real-world data in place of simulations– What fraction of 3-person mixtures look like 2-person mixtures?
– How evenly distributed is DNA profile space?
![Page 15: The Time Has Come to Analyze DNA Profile Databases Forensic Bioinformatics () Dan E. Krane, Ph.D., Wright State University, Dayton,](https://reader035.vdocuments.us/reader035/viewer/2022062805/5697c01c1a28abf838ccff39/html5/thumbnails/15.jpg)
It is time for disclosure of the NDIS database
• Open access to data is a fundamental tenet of science.
• Anonymized records would be easily copied and pose little (if any) privacy risk.
• Time for DNA disclosure– Science, 2009; 326:1631-1632.