the scientific method: tutorial start from beginning designing the experiment variable practice...

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The Scientific Method: Tutorial Start from beginning Designing the experiment Variable practice Graphing the data Conclusion

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The Scientific Method: Tutorial

Start frombeginning

Designing theexperiment

Variablepractice

Graphing thedata

Conclusion

Introduction: The Scientific MethodThe scientific method is a series

of steps used by scientists to help them solve problems. A valid experiment will identify a problem, research the problem to see if others have collected data for similar problems, create a hypothesis, collect data by performing an experiment, analyze the data collected, and report the findings.

.

Experimental Design

• Setting up the experiment can be the toughest part of the scientific method for young scientists. A poorly designed experiment can make your data hard to understand and even meaningless. Therefore, the purpose of this tutorial is to help you learn basic fundamentals to help you design an experiment and interpret the data collected.

Identifying a ProblemSimons-mycin (fictional) is a prescription

medication designed to relieve arthritis pain.

Jane and Jerry are both young doctors who have heard rumors that Simons-mycin can raise the blood pressure of patients who use the drug. This can be a serious medical concern because if the patients’ blood pressure is too high, they can suffer a heart attack and possibly die. This is the first stage of the scientific method, identify a problem.

What is the problem that Jane and Jerry have identified?

Some patientstake Simons-mycin.

Some patients havehigh blood pressure.

Some patients areat risk of heart attack.

Some patients whotake Simons-mycin may

have higher blood pressures.

But what is the connection? But what is the connection?

This information is not given in the story.

Correct.

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Researching the ProblemJane and Jerry have agreed to meet at the UCLA medical library to research their

problem. They searched countless journals to see if other doctors have

performed work on the effects of

Simons-mycin, but they did not find

the answer to their problem question.

They will need to perform their own

experiment.

Even though Jane and Jerry have discussed the risks with their patients about the use of Simons-mycin, they have found volunteers to test the medication. Together, Jane and Jerry come up with a plan to test Simons-mycin and the possible blood pressure risk.

Now that Jane and Jerry have identified their problem and performed research, it is time for them to create a hypothesis that answers their problem. Remember, the problem they are trying to answer is:

“Does Simons-mycin raise the blood pressure of their patients?”

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Now that Jane and Jerry have identified their problem and performed research, it is time for them to create a hypothesis that answers their problem. Remember, the problem they are trying to answer is:“Does Simons-mycin raise the blood pressure of their patients?”

Creating the HypothesisNow that Jane and Jerry have identified their problem and performed research, it is time for

them to create a hypothesis that answers their problem. Remember, the problem they are trying to answer is: “Does Simons-mycin raise the blood pressure of patients?”

The hypothesis is a possible solution to this problem and should be worded as an “If…then” statement. Click on the box below that would be a possible solution to this problem. Which will be our hypothesis?

If a person usesSimons-mycin, then theymay have higherthan normalblood pressure.

If a person isoverweight, then theymay have higherthan normalblood pressure.

If a personhas high bloodpressure, then theymust use Simons-mycin.

This may be true also but Jane and Jerry are not testing a person’s weight. This could be another experiment to perform at a later date though.

There is no reason to believe this. There are numerous reasons why a person could have high blood pressure.

Correct. Press the NEXT button when ready.

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Designing the ExperimentJane and Jerry are ready to design and perform their experiment to test their hypothesis. But how

do you design an experiment in the first place? Keep in mind our problem question:

“Does Simons-mycin affect a person’s blood pressure?”

Their experiment will need to test the blood pressure of volunteers. Jane and Jerry have found 50 patients who are willing to volunteer for this experiment. Valid experiments usually create two groups of test subjects.

One group is called the CONTROL group. Participants of this group are not given the desired treatment (Simons-mycin). Therefore, the control group is used as a comparison group.

The second group is called the EXPERIMENTAL group. Participants of this group are given the desired treatment (Simons-mycin). The experimental subjects will be compared to the control group to see if the treatment worked or not.

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Designing the ExperimentJane and Jerry decide to separate the 50 people randomly into two groups of 25 patients each.

Group A:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were not given Simons-mycin.

The 25 patients were instructed to eat and exercise according to specific instructions.

After one month, the 25 patients would return to have their blood pressures checked again.

Group B:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were given typical doses of Simons-mycin.

The 25 patients were instructed to eat and exercise according to the same instructions as group A.

After one month, the 25 patients would return to have their blood pressures checked again.

Controlgroup

Experimentalgroup

Is Group A the control group or the experimental group?

Not quite. Remember that the experimental group is given the treatment. In this case, group A is not given Simons-mycin.

Correct. Because no treatment was given to Group A, that makes them the control group.

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Designing the ExperimentJane and Jerry decide to separate the 50 people randomly into two groups of 25 patients each.

Controlgroup

Experimentalgroup

Is group B considered the control group or the experimental group?

Correct. Since group B received the treatment (Simons-mycin) they are the experimental group.

Not quite. The control group is the one that does not receive treatment. Group B does receive treatment.

Group A:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were not given Simons-mycin.

The 25 patients were instructed to eat and exercise according to specific instructions.

After one month, the 25 patients would return to have their blood pressures checked again.

Group B:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were given typical doses of Simons-mycin.

The 25 patients were instructed to eat and exercise according to the same instructions as group A.

After one month, the 25 patients would return to have their blood pressures checked again.

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Identifying Variables

What is different between Group A and Group B?

(What is the independent variable of this experiment?)

Number ofpeople

Diet habitsMedication

takenExercise

habits

The independent variable is the factor that differs between the control group and experimental group. A good experiment should only have 1 difference between the two groups. If there were accidentally more than 1 independent variable and the patients blood pressure changed, we would not know which factor was responsible.

No. Each group had 25 people.

No. Each group followed the same habits.

No. Each group followed the same habits.

Correct.

Group A:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were not given Simons-mycin.

The 25 patients were instructed to eat and exercise according to specific instructions.

After one month, the 25 patients would return to have their blood pressures checked again.

Group B:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were given typical doses of Simons-mycin.

The 25 patients were instructed to eat and exercise according to the same instructions as group A.

After one month, the 25 patients would return to have their blood pressures checked again.

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Identifying Variables

Bodyweight

Bloodpressure

Simons-cyoltaken

Heartrates

The data measured will be the dependent variable. What is the dependent variable?

No. Body weights are never mentioned or collected.

No. This is the independent variable because it is what differs between group A and group B.

No. Heart rates are never mentioned or collected.

Correct. It is called the dependent variable because the patient’s blood pressure may depend on the use of Simons-mycin.

Group A:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were not given Simons-mycin.

The 25 patients were instructed to eat and exercise according to specific instructions.

After one month, the 25 patients would return to have their blood pressures checked again.

Group B:

To start, the 25 patients had their blood pressures recorded.

Then, the 25 patients of this group were given typical doses of Simons-mycin.

The 25 patients were instructed to eat and exercise according to the same instructions as group A.

After one month, the 25 patients would return to have their blood pressures checked again.

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Variable Practice: Farmer BobBefore proceeding, let’s stop for a moment and get some practice identifying variables and parts of an

experiment.

Farmer Bob has always used Grow-Rite Fertilizer on his crops, but wants to try Ever-Grow Fertilizer. He sections off two large areas of his field. In section A, he waters his crops as usual and adds Grow-Rite. In section B, he waters his crops as usual but adds Ever-Grow fertilizer. After 1 growing season, he records the growth of the crops.

What is the independent variable of Farmer Bob’s experiment?

The type offertilizer used

Group A crops Group B cropsThe height of

the crops

Correct.Hmmm. Remember the independent variable is what differs between the two groups. Try again.

Hmmm. Remember the independent variable is what differs between the two groups. Try again.

Hmmm. Remember the independent variable is what differs between the two groups. Try again.

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The height ofthe crops

Correct.

Variable Practice: Farmer BobBefore proceeding, let’s stop for a moment and get some practice identifying variables and parts of an

experiment.

Farmer Bob has always used Grow-Rite Fertilizer on his crops, but wants to try Ever-Grow Fertilizer. He sections off two large areas of his field. In section A, he waters his crops as usual and adds Grow-Rite. In section B, he waters his crops as usual but adds Ever-Grow fertilizer. After 1 growing season, he records the growth of the crops.

What is the dependent variable of Farmer Bob’s experiment?

The type offertilizer used

Group A crops Group B crops

Hmmm. Remember the dependent variable is the data collected. Try again.

Hmmm. Remember the dependent variable is the data collected. Try again.

Hmmm. Remember the dependent variable is the data collected. Try again.

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The United States military wants to see if soldiers with only 4 hours of sleep can operate at the same level as those who normally get 7 hours of sleep. One hundred soldiers are allowed to only sleep for 4 hours a night and another hundred are allowed to sleep for 7 hours a night. During the day, the soldiers are tested for marksmanship on the firing range.

What is the independent variable of the military experiment?

Variable Practice: USA Military

The ammunitionused

Amount ofsleep given

Score on thefiring range

The numberof soldiers

Correct.

Hmmm. Remember the independent variable is what differs between the two groups. Try again.

Hmmm. Remember the independent variable is what differs between the two groups. Try again.

Hmmm. Remember the independent variable is what differs between the two groups. Try again.

..

.

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Variable Practice: USA MilitaryThe United States military wants to see if soldiers with only 4 hours of sleep can operate at the

same level as those who normally get 7 hours of sleep. One hundred solders are allowed to only sleep for 4 hours a night and another hundred are allowed to sleep for 7 hours a night. During the day, the soldiers are tested for marksmanship on the firing range.

What is the control group of the military experiment?

Soldiers with7 hours sleep

Soldiers with4 hours sleep

Score on thefiring range

The numberof soldiers

Correct.

Hmmm. Remember the control group are those who are not given the experimental treatment. Try again.

Hmmm. Remember the control group are those who are not given the experimental treatment. Try again.

Hmmm. Remember the control group are those who are not given the experimental treatment. Try again.

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Variable Practice: USA MilitaryThe United States military wants to see if soldiers with only 4 hours of sleep can operate at the

same level as those who normally get 7 hours of sleep. One hundred solders are allowed to only sleep for 4 hours a night and another hundred are allowed to sleep for 7 hours a night. During the day, the soldiers are tested for marksmanship on the firing range.

What is the dependent variable of the military experiment?

The ammunitionused

Amount ofsleep given

Score on thefiring range

The numberof soldiers

Correct.Hmmm. Remember the dependent variable is the data collected. Try again.

Hmmm. This was the independent variable. Try again.

Hmmm. Remember the dependent variable is the data collected. Try again.

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Back to Our Experiment

At the start of the experiment, remember that everyone had their blood pressures measured. On average, both the control group and the experimental group had a systolic blood pressure of 120. This is a fairly healthy blood pressure. Click the “? Buttons” below to continue.

Let’s look at the blood pressures at

the start of this experiment.

Where’s the data Jerry?

I have it right here Jane.

Control group Experimental group

Start 120 mm Hg 120 mmHg

End

? ?

Did you click both boxes?If so, click here next.

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Sun Mon Tues Wed Thurs Fri Sat

1Take initial blood pressures

2 3 4 5

6 7 8 9 10 11 12

13 14 15 16 17 18 19

20 21 22 23 24 25 26

27 28 29 30Take final blood pressures

Day 2 Day 3 Day 4 Day 5

Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12

Day 13 Day 14 Day 15 Day 16 Day 17 Day 18 Day 19

Day 20 Day 21 Day 22 Day 23 Day 24 Day 25 Day 26

Day 27 Day 28 Day 29

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1 Month Later

After one month all 50 people returned to have their blood pressure examined. Click the “?” boxes below to reveal the results.

Those who were not using Simons-mycin maintained their healthy blood pressure of 120. However, those who used Simons-mycin now had an average blood pressure of 145. Time to make our graph.

I’m so anxious. I hope our

hypothesis was valid.

Finally our experiment is

finished. It’s been 30 days. Let’s see

the data.

Control group Experimental group

Start 120 mm Hg 120 mm Hg

End 120 mm Hg 145 mm Hg? ?

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Affects of Kobe-Cyol on blood pressure

Graphing the DataA title needs to be clear to a reader.

Avoid titles such as “Biology graph” and “Our data” because these titles don’t tell us what the graph really shows. Click on the good title.

Now we need to label the X and Y axis.Graphs makes

our data easier to understand. Pictures are

worth a thousand words after all.

Others will read our results once

we share our findings.

We better be clear about the data. Let’s start

with the title.

Affects of Simons-mycin on blood pressure

The results of a month long test

No. This is too vague.

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Patients who used Kobe-cyol for 1 month

Patients who did not use Kobe-cyol

Blo

od p

ress

ure

valu

es (

mm

Hg)

Affects of Simons-Cyol on blood pressure

Graphing the DataAgain. Be specific. Which label

is specific on the Y axis?

Now for the X axis. Which is specific?

Amount

Blood pressure values(mm Hg)

Amount of what? This is

too vague.

Patients who used Patients who didSimons-mycin for 1 month not use Simons-mycin

Two groups of people

Be specific.

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Blo

od p

ress

ure

valu

es (

mm

H

g)

Affects of Simons-mycin on blood pressure

Graphing the DataKeep in mind our data.

Control group:

Start: 120

End: 120

Experiment group:

Start: 120

End: 145

We must use equal intervals to number the Y axis.

180

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120

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40

30

20

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0 Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

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Affects of Kobe-Cyol on blood pressure

Graphing the DataSince we have before and

after results, we need two different bar patterns.

Striped bars will be the start of data collecting.

Dotted bars will be the end of the data collection.

So now we need to add a key so the readers of our graph know this.

180

170

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0

KEY

stripes = start of the test

dots = end of the test

Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

back

Blo

od p

ress

ure

valu

es (

mm

H

g)

Affects of Simons-Cyol on blood pressure

Graphing the DataNow we are ready to fill in

the graph itself. Let’s graph the people who used Simons-mycin first.

People who used Simons-cyol

(Experiment group):

Start: 120

End: 145

180

170

160

150

140

130

120

110

100

90

80

70

60

50

40

30

20

10

0

KEYstripes = start of the test

dots = end of the test

Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

back

Blo

od p

ress

ure

valu

es (

mm

H

g)

Affects of Kobe-Cyol on blood pressure

Graphing the DataNow let’s graph the people

who did not use Simons-mycin

People who did not use Simons-mycin

(Control group):

Start: 120

End: 120

180

170

160

150

140

130

120

110

100

90

80

70

60

50

40

30

20

10

0

KEYstripes = start of the test

dots = end of the test

Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

back

Blo

od p

ress

ure

valu

es (

mm

H

g)

Affects of Simons-mycin on blood pressure

What is the data telling us?To determine what could have

caused the jump in blood pressure, we need to examine what was different between the two groups of people?

Number of people

Exercise habits

Diet habits

Length of testing time

Type of medication taken

180

170

160

150

140

130

120

110

100

90

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0

KEYstripes = start of the test

dots = end of the test

samedifferent

samedifferent

samedifferent

samedifferent

samedifferent

correct

correct

correct

correct

correct Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

back

Blo

od p

ress

ure

valu

es (

mm

H

g)

Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

Affects of Simons-Cyol on blood pressure

What is the data telling us?Number of people

Exercise habits

Diet habits

Length of testing time

Type of medication taken

If the only change between the two groups was the type of medication taken, then that must be the reason for the increased blood pressure. I hope you see the importance of only 1 independent variable.

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KEYstripes = start of the test

dots = end of the test

same

same

same

same

different

back

Blo

od p

ress

ure

valu

es (

mm

H

g)

Patients who used Simons-mycin for 1 month

Patients who did not use Simons-mycin

Affects of Simons-mycin on blood pressure

What about the Hypothesis?Now that our graph is finished, what does it tell us about our hypothesis? If you forgot, the

hypothesis was: If a person uses Simons-mycin, then they may have higher than normal blood pressure.

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KEYstripes = start of the test

dots = end of the test

The hypothesis was proven correct.

The hypothesis appears valid.

The hypothesis is proven wrong.

The hypothesis appears wrong.

One experiment proves nothing. More tests are required to state this.

Correct.

Are you sure? The data agrees with the prediction.

Are you sure? The data agrees with the prediction.

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Blo

od p

ress

ure

valu

es (

mm

H

g)

Stating a Conclusion

A conclusion should be a clear and understandable summary of the main idea learned from the experiment. Be straight to the point. What would a good summary be?

Wow! I know it is only 1 experiment,

but we need to report our findings

so others can check our work.

I think we should take the 25 patients off Simons-mycin immediately and

continue to monitor their health.

Simons-mycin was the independent variable of this experiment.

Simonsmycinl makes people sick and unhealthy.

Our experiment was valid and well deigned.

Simons-mycin appears to raise the blood pressure of normal individuals.

This may be true, but it is not the main idea learned.

This may be true, but it is too vague.

This may be true, but it is too vague.

Correct

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Reporting your resultsIn order for others to confirm our findings, our results need to be shared with others. There are

many journals that collect research performed by scientists around the world. Even newspapers and magazines will often pick up on a study of great importance. This is how information is spread around the world about various scientific findings.

Experiments are valid when the rules of the scientific method have been followed. This method reduces errors, eliminates bias, and advances our understanding of the world in which we live.

Let’s Review:

#1) What is a tentative solution to a problem called?

hypothesisindependent

variabledependent

variablecontrol group

Correct.

Try again. This is the data collected.

Try again. This is the factor differing in an experiment.

Try again. This is the comparison group.

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Review #2Which variable differs between the control group and the experimental group?

Dependent variable Independent variable

This is the data that is collected because of the change.

Correct

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Review #3Which hypothesis best fits the problem question below?

Problem question: Why do some people develop diabetes?

A diet that is high insugar can lead to diabetes.

Diabetes is caused bya high sugary diet.

If a person has a diet that is high in sugar,then that may lead to diabetes.

That may answer the question, but it is not worded in proper format.

That may answer the question, but it is not worded in proper format.

Correct.

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Review #4Pretend our control group had 35 people exercise according to a different plan than the

25 people of the experimental group who still took Simons-mycin for 1 month. How many variables would be in this experiment?

ONE THREETWO

Correct.1) Different number

of people2) Different exercise

program3) Use of Simons-

mycin

Recount. How many times do the control group and experimental group differ according to the question?

Recount. How many times do the control group and experimental group differ according to the question?

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Review #5Pretend our control group had 35 people exercise according to a different plan than the

25 people of the experimental group who still took Simons-mycin for 1 month. Why is this a poorly designed experiment?

There are toomany differentvariables betweenthe control andexperimentalgroups.

There are noindependentvariablesto thisexperiment.

The dependentvariable isnot differentfrom thecontrol group.

Correct. Valid experiments only differ once.

This statement is confusing actually. How many differences should exist between the control and experimental groups?

That’s not true. There are actually multiple variables.

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The end!You will be expected to use the scientific method throughout the course of the school

year. I hope this tutorial simplified how it can help solve problems. Be sure your name is on your paper before submitting your work.

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