the rapid microbiology company rapid microbiology results for food products challenges and solutions...

The Rapid Microbiology Company

Rapid Microbiology Results

For FOOD Products

Challenges and Solutions

Copyright AES-Chemunex 2008Pascal Yvon, Pharm.D., MBA


Manage Your Quality During

the Whole Production Process

Production Process:Production Process:From Farm to Finished ProductsFrom Farm to Finished Products


From Farms From Farms Factories Factories Finished Finished ProductsProducts

TO TO


Testing & Release of

Finished Products

Testing of

Raw Materials

& Water

In-Process Testing(Blending, Filling,

Pasteurization, etc.)

& Cleaning Validation

Critical Control Points (CCPs) Critical Control Points (CCPs) In the Production ProcessIn the Production Process



Finished productsRaw Materials In-Process Testing

Risk-Based Analysis:Risk-Based Analysis:Impact of Microbial ContaminationImpact of Microbial Contamination

Unloading contaminated trucks

Low quality of raw materials

Contamination of storage tanks

Formation of biofilms

High sanitization costs

Plant downtime

Loss of in-process products

High cost of re-treatment

Contamination of tanks

Plant downtime

High cost of cleaning

Loss of contaminated batches

Back orders

Loss of business

Recalls

Damage to company image

Waste of Time / Loss of Money




QA ChallengesQA Challenges

Real -time control of raw materials before unloading

Real-time control of water

Fast analysis of bulk products before filling

Optimize pasteurization conditions according to microbial content

Fast detection, localization & correction of contamination

Decrease warehouse costs

Increase production flexibility

Decrease product losses

Minimize production disruption

Protection of company image

Improved delivery time

Proactive Actions in QA to Save Time & Money



Traditional microbiology results are based on plate-count methods, which can take 3 to 7+ days until results are available

Slow results

Retrospective to production schedules

Results vary with microbial population, media & conditions

Spores & stressed cells require extended growth periods

Products need to be kept in quarantine until micro results are available

Raw materials can not be used until micro lab delivers results

Need: Faster Results from the Micro Need: Faster Results from the Micro LabLab



Advantage: Rapid Results Within 24 or Advantage: Rapid Results Within 24 or 48 Hours48 Hours



Solution:Solution: Real-Time Microbiology Real-Time Microbiology TestingTesting

Minimize

contamination risks

Control raw materials

before use

Implement just-in-time

production

Remediate problems in

real-time

Minimize holding time

Release products

earlier

Reduce cost for

product storage

Minimize losses

Guarantee product

quality




Benefits of Rapid MicrobiologyBenefits of Rapid Microbiology

Save Time & Money with Total QA

Direct count raw material within 20min

Direct count water within 20min

Save 2 to 4 Days

Total Time Savings over the Entire Production Cycle:

6 TO 12 DAYS

Direct count surface within 20min

Presence/Absence TVC 24h

Save 2 to 4 Days

Presence/Absence TVC 24h

Save 2 to 4 Days



Rapid Microbiology TestingRapid Microbiology Testing

Using Ultra-sensitive Using Ultra-sensitive Flow-cytometersFlow-cytometers

AES-CHEMUNEX: Unique SolutionsAES-CHEMUNEX: Unique Solutions



AES-Chemunex: Labeling TechnologyAES-Chemunex: Labeling Technology

CRITERIA OF VIABILITY Enzyme activity Membrane integrity

Enzyme

Viability substrate

Free fluorochrome

Microorganism

APPLICABLE FOR Total Viable Count Yeast & Molds Enterobacteriaceae (selective enrichment) Salmonella (selective enrichment and immunologic labeling)


DIRECT LABELING


AES Chemunex Detection Technology: Flow Cytometry

Detector 1

Detector 2

Waste

Laser

Sample

Designed for industrial microbiology testing

Sample passes flowcell in a linear fluid stream

Microorganism cells are illuminated by 488 nm laser beam

Emmitted

fluorescence is collected by sensitive detectors

Microorganism

cells are counted directly one by one


DIRECT DETECTION & ENUMERATION


Results Directly In Microbial Cell Results Directly In Microbial Cell CountsCounts

No Calibration

No Interpretation



Direct Count Test & Presence-Absence Direct Count Test & Presence-Absence TestTest

Raw materials Non-sterile products Process water

Sensitivity: 100+ cells per ml*

Time: Result available 15 min

after sampling for a

single sample

* application dependent

DIRECT COUNT METHOD

Final-product testing

Sterile products

PRESENCE/ABSENCE METHOD

Sensitivity: 1 microorganism per pack*

Time: Result available 15 min

after pre-incubation and sampling (single sample)

Pre-incubation: 24 - 72 hours in pack*

* application dependent



- fully automated analyzer

- up to 350 samples per working shift

- throughput 50 samples/h (application dependent)

- capacity 1 - 50 samples per batch

- fully automated analyzer

- up to 150 samples per day - throughput 20 samples/h

(application dependent)

- capacity 1-25 samples per batch

- semi-automatic analyzer

- up to 50 samples per day

- 1 to 10 samples per batch

AES-Chemunex: Rapid Microbiology AES-Chemunex: Rapid Microbiology SystemsSystems

D-Count

BactiFlow ALS

BactiFlow



ApplicationsApplications

Dairy Beverages

Salad, meat, etc.

Water



AES-Chemunex: Combining Speed AES-Chemunex: Combining Speed with Sensitivitywith Sensitivity

Easy-to-use systems, designed for industrial

microbial use

Rapid results

Direct Count results within a few minutes after

sampling

Presence / Absence test for sterile samples with

typically 24-48 hours pre-incubation of

sample

Direct Counting and Presence/Absence Test

Quantitative Direct Count Method: counting cell by

cell

No calibration curve required, no data interpretation

Direct correlation with traditional plate count, easy

to validate

High Sensitivity

Large Linear Counting: up to 500,000

microorganisms/ml labeled solution



Rapid Microbiology: BenefitsRapid Microbiology: Benefits

SENSITIVITY TO ONE CELL, ACCURACY & SPEED

DIRECT COUNTING OF MICROBIAL CELLS

HIGH CAPACITY & THROUGHPUT, MINIMUM OPERATOR TIME,

NO INTERPRETATION

Get results in hours

Test raw materials, in-process & final products in real time

Release final products in hours, not days

Extend product shelf life by days

Reduce warehousing space & costs

Reduce production downtimes & contamination risks

Detect & respond to contamination risks immediately

Increase production flexibility & productivity

Increase profitability

Safeguard product quality, reputation & customer satisfaction



Companies Using Rapid MicrobiologyCompanies Using Rapid Microbiology


Antartic

BINA Bischofszell

Cadbury Schweppes

Coca Cola

Cott

Dannon

Délifruits

Del Monte

Eckes Granini

Emig Gerber

Frutarom

Fruchtquell-

Gerolsteiner

Hassia

Mineralbrunnen

Hochwald Sprudel

Joker

Kerry Aptunion

Pfanner

Rauch Fruchsäfte

Rivella

Dr. Siemer Getränke

Sill

Wesergold

Wild, Wild Flavors

Zuegg


Contact: Pascal Yvon

Tel. 609 497 [email protected]

om

AES – Chemunex, Inc.301 N. Harrison Street,

Suite 109Princeton, NJ 08540Fax 609 497 7307

www.aeschemunex.com

Copyright AES-ChemunexPascal Yvon, Pharm.D., MBA, 2008

Pascal Yvon, PharmD, MBA

CEO of AES-Chemunex, Inc.

Princeton, NJ

the rapid microbiology company rapid microbiology results for food products challenges and solutions...

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